最新刊期
      卷 11 ， 期 5 ， 2005
      • PENG Li-hua,LIU Shu-zhi,GUO Chun-yan,LI Man-ling,FENG Wei-hong,KANG Chen,JIN Ri-xian
        Vol. 11, Issue 5, Pages: 1-3(2005)
        摘要：Objectives:To compare the releasing rates in vitro of evodiamine and rutaecarpine in two kinds of cataplasma carriers.Methods:The releasing of evodiamine and rutaecarpine from the cataplasma carriers in vitro were measured,imitating the method of percutaneous absorption by using improved Franz'S diffusion cell and HPLC.Results:The releasing rates of Evodiamine and rutaecarpine in the carrier made of acryl ic resin copotymer and natural copolymers are higher than those in the carrier made of acrylic resin coDolymer and compounded copolymers.And both the releasing processes fit to the Higuchi equation.Conclusion:Both the cataplasma carriers have good drug releasing properties.  
        关键词：Percutaneus absorption;Cataplasma;Evodiarrvine;rutaecarpine;HPLC   
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        发布时间：2024-09-23
      • PENG Li-hua,LIU Shu-zhi,GUO Chun-yan,LI Man-ling,FENG Wei-hong,KANG Chen,JIN Ri-xian
        Issue 5, Pages: 1-3(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.001
        摘要：Objectives:To compare the releasing rates in vitro of evodiamine and rutaecarpine in two kinds of cataplasma carriers.Methods:The releasing of evodiamine and rutaecarpine from the cataplasma carriers in vitro were measured,imitating the method of percutaneous absorption by using improved Franz’S diffusion cell and HPLC.Results:The releasing rates of Evodiamine and rutaecarpine in the carrier made of acryl ic resin copotymer and natural copolymers are higher than those in the carrier made of acrylic resin coDolymer and compounded copolymers.And both the releasing processes fit to the Higuchi equation.Conclusion:Both the cataplasma carriers have good drug releasing properties.  
        关键词：Percutaneus absorption;Cataplasma;Evodiarrvine;rutaecarpine;HPLC   
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        发布时间：2024-09-23
      • Issue 5, Pages: 3-5(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.002
        摘要：目的考察超滤膜截留分子量对超滤效果的影响,并与醇沉法进行比较。方法采用超滤法（膜截留分子量2万、7万）和乙醇沉淀法纯化双黄连口服液。以金银花有效成分绿原酸保留率、杂质多糖和鞣质的清除率、干浸膏粉量以及膜通量变化为指标,对两种膜超滤效果进行对比,并与乙醇沉法进行比较。结果绿原酸保留率两种截留分子量的膜相差不大,但明显高于醇沉;去除多糖以2万膜最好,其次为7万膜,醇沉最差;去除鞣质无明显差异;干浸膏粉量7万膜与醇沉相差不大,均高于2万膜;两种膜通量衰减程度相差不大,但7万膜由于孔径大,超滤等量药液所需时间缩短一倍。结论选择适宜的超滤膜截留分子量可以达到纯化中药的效果。  
        关键词：超滤;醇沉;膜截留分子量;双黄连口服液   
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        发布时间：2024-09-23
      • Issue 5, Pages: 6-7(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.003
        摘要：采用正交设计法,以人参皂苷Rg1和人参皂昔Re的总含量为考察指标,对维康胶囊的加醇量、提取次数、提取时间三因素进行优选研究。确定了合理可行的提取工艺条件,即加70%乙醇量为24倍量（8,8,8）,回流3次,每次2h。并确定了维康胶囊的成型工艺。  
        关键词：维康胶囊;正交设计;人参皂苷Rg1;人参皂苷Re;成型工艺   
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        发布时间：2024-09-23
      • Issue 5, Pages: 8-9(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.004
        摘要：目的建立麻杏石甘汤中麻黄碱与伪麻黄碱的HPLC分离及测定方法;方法HPLC条件YMCODSC184μm×150mm,流动相H2OCH3CNSDSH3PO4（650∶350∶5∶1）,柱温50℃,检测波长210nm,流速1.5mL/min;结果在该分离条件下,可以将麻黄碱与伪麻黄碱分离,并可以同时进行测定;结论建立了麻黄碱与伪麻黄碱在麻黄药材以及麻杏石甘汤中的HPLC色谱分离及测定方法。  
        关键词：高效液相色谱法;麻杏石甘汤制剂;麻黄碱、伪麻黄碱   
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        发布时间：2024-09-23
      • Issue 5, Pages: 10-12(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.005
        摘要：目的测定中药复方五虎汤中伪麻黄碱、麻黄碱的溶出率。方法用50%甲醇水超声提取样品,在ODS柱上以40%乙腈水（以H3PO4调pH为2.2,加0.5%的SDS）为流动相,在波长210nm处紫外检测。结果测得麻黄碱、伪麻黄碱的溶出率分别为86.3%、89.4%。结论中药复方五虎汤中伪麻黄碱、麻黄碱有较高的溶出率。  
        关键词：伪麻黄碱;麻黄碱;溶出率   
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        发布时间：2024-09-23
      • ZHONG Zhao-jian,SONG Fen-Yun,LI Shu-yuan,WU Fu-Hai,CHEN Cui-ling
        Vol. 11, Issue 5, Pages: 12-14(2005)
        摘要：Objective:To study the quality standsard of Radix Fici simplicissimatis.Methods:Psoralen in Radix Fici simplicissimatis was identified by TLC.A RP-HPLC method was established to determine the content of psoralen in Radix Fici simplicissimatis.Results:The concentration of psoralen is linearly related to its peak area in the range of 7.44～38.60 μg·mL-1(r=0.99997),and the average recovery was 99.2% with RSD=2.15%.Precision of the method was 2.50%(RSD,n=6).Conclusion:The method is simple,accurate and reliable for quality control of this crude drug.  
        关键词：Radix Fici simplicissimatis;Psoralen;TLC;A RP-HPLC   
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        发布时间：2024-09-23
      • ZHONG Zhao-jian,SONG Fen-Yun,LI Shu-yuan,WU Fu-Hai,CHEN Cui-ling
        Issue 5, Pages: 12-14(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.006
        摘要：Objective:To study the quality standsard of Radix Fici simplicissimatis.Methods:Psoralen in Radix Fici simplicissimatis was identified by TLC.A RP-HPLC method was established to determine the content of psoralen in Radix Fici simplicissimatis.Results:The concentration of psoralen is linearly related to its peak area in the range of 7.44～38.60 μg·mL -1 （r=0.99997）,and the average recovery was 99.2% with RSD=2.15%.Precision of the method was 2.50%（RSD,n=6）.Conclusion:The method is simple,accurate and reliable for quality control of this crude drug.  
        关键词：Radix Fici simplicissimatis;Psoralen;TLC;A RP-HPLC   
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        发布时间：2024-09-23
      • LI Zhi-wan1,MA Qiang2,YUAN Yong-sheng3,ZHOU Yu-xin3
        Vol. 11, Issue 5, Pages: 14-16(2005)
        摘要：Objective:To develop a method for the determination of hyodeoxycholic acid in Porcine Gall Powder.Method:Chromatography was performed on a 5 μm Kromasil C18(4.6 mm×250 mm)at 25℃.The mobile phase was composed of acetonitrile-0.1% acetic acid solution (50∶50)with a flow rate of 1.0mL·min-1.A Sedex 55 evaporative light-scattering detector was used with tube temperature of 40℃ and gas pressure of 2.0 bar.Result:A good relationship between peak area and amount of injection Was noted at 5.4 μg～27.0 μg.Average recovery of hyodeoxycholic acid was 97.0%and the RSD was 2.7%.Conclusion:It was proved that the method was reliable,simple and accurate.  
        关键词：HPLC-ELSD;Porcine Gall Powder;hyodeoxycholic acid   
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        发布时间：2024-09-23
      • LI Zhi-wan1,MA Qiang2,YUAN Yong-sheng3,ZHOU Yu-xin3
        Issue 5, Pages: 14-16(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.007
        摘要：Objective:To develop a method for the determination of hyodeoxycholic acid in Porcine Gall Powder.Method:Chromatography was performed on a 5μm Kromasil C 18 （4.6mm×250 mm）at 25℃.The mobile phase was composed of acetonitrile-0.1% acetic acid solution （50∶50）with a flow rate of 1.0mL·min -1 .A Sedex 55 evaporative light-scattering detector was used with tube temperature of 40℃ and gas pressure of 2.0 bar.Result:A good relationship between peak area and amount of injection Was noted at 5.4μg～27.0μg.Average recovery of hyodeoxycholic acid was 97.0%and the RSD was 2.7%.Conclusion:It was proved that the method was reliable,simple and accurate.  
        关键词：HPLC-ELSD;Porcine Gall Powder;hyodeoxycholic acid   
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        发布时间：2024-09-23
      • Issue 5, Pages: 16-17(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.008
        摘要：目的研究通脉灵胶囊的质量控制方法。方法采用TLC定性鉴别;采用HPLC测定制剂中的酪氨酸含量。结果通脉灵胶囊与穿山甲对照药材在薄层的相同位置处显示出相同斑点;酪氨酸的线性范围是0.16～0.96μg,r=0.9999;平均回收率为95.51%,RSD为1.38%;通脉灵胶囊中酪氨酸的含量是11.36%。结论该方法灵敏、简便、重复性好,可作为该制剂的质量控制方法。  
        关键词：通脉灵胶囊;酪氨酸;TLG;HPLG;质量控制   
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        发布时间：2024-09-23
      • Issue 5, Pages: 18-20(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.009
        摘要：目的建立青羚散中人工牛黄的含量测定方法。方法采用反相高效液相法进行人工牛黄中胆酸的含量测定,采用HypersilBDSC18色谱柱（250×4.6mm,5μm）;以甲醇1%醋酸溶液（77∶23）为流动相,流速1.0mLmin;柱温40℃;采用蒸发光散射检测器,检测温度40℃;灵敏度10;氮气压力1.0bar。结果线性范围0.49～3.92μg,相关系数r=0.9992。平均回收率为100.5%（n=6）,RSD=1.79%。结论该方法为评价和控制青羚散的质量提供了依据。  
        关键词：青羚散;人工牛黄;胆酸;HPLC-ELSD;含量测定   
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        发布时间：2024-09-23
      • Issue 5, Pages: 20-21(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.010
        摘要：目的建立八珍胶囊中芍药苷的含量测定方法。方法HPLC法,流动相甲醇0.05molL磷酸二氢钾溶液（28∶72）;检测波长为230nm;柱温30℃。结果芍药苷线性范围为0.32μg～1.89μg,平均回收率99.1%,RSD为1.57%。结论方法可靠,简单可行,为控制八珍胶囊的内在质量提供了科学依据。  
        关键词：八珍胶囊;芍药苷;高效液相色谱法   
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        发布时间：2024-09-23
      • LI Yuan-hong,CHEN Ji-yan,ZHU Hai-tao,AN Zhi-bin
        Vol. 11, Issue 5, Pages: 22-23(2005)
        摘要：Objective:To establish the HPLC method for determination of icariin in Gushen pills.Method:HPLC was performed on a Diamonsil C18column (4.6×200 mm,5μm) with acetonitrile-water(20∶80) as mobile phase.Detecting wavelength was 270nm and flow rate was 1.0mL/min.Result:A good linear relationship between peak area and concentration was noted at 0.15～2.10 μg with a correlation coefficient of r=0.99998(n=6) for icariin.The recovery rate of icariin was 97.4%(RSD=2.17%).Conclusion:This method is accurate, repeatable and effective for quality control of Gushen pills.  
        关键词：Gushen pills;icariin;HPLC;content   
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        发布时间：2024-09-23
      • LI Yuan-hong,CHEN Ji-yan,ZHU Hai-tao,AN Zhi-bin
        Issue 5, Pages: 22-23(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.011
        摘要：Objective:To establish the HPLC method for determination of icariin in Gushen pills.Method:HPLC was performed on a Diamonsil C 18 column （4.6×200mm,5μm） with acetonitrile-water（20∶80） as mobile phase.Detecting wavelength was 270nm and flow rate was 1.0mL/min.Result:A good linear relationship between peak area and concentration was noted at 0.15～2.10μg with a correlation coefficient of r=0.99998（n=6） for icariin.The recovery rate of icariin was 97.4%（RSD=2.17%）.Conclusion:This method is accurate, repeatable and effective for quality control of Gushen pills.  
        关键词：Gushen pills;icariin;HPLC;content   
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        发布时间：2024-09-23
      • WENG Xiao-gang,NIE Shu-qin,YANG Qing,HUANG Lu-qi
        Issue 5, Pages: 24-27(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.012
        摘要：Objective:To probe the counteractiv emechanism of “aconite counteracts fritillaria”.Methods:We used Solid phase extraction（SPF）to extract and enrich the components contained in the plasma.Chromatographic condition: Methyl alcohol-water-chloroform-triethylamine（70∶30∶2∶0.2）was used as the mobile phase, the flow rate was 1.0mL·min -1 with detection wavelength at 235nm and column temperature at 40℃; detecting the contents of mesaconitine, aconitine and hypaconitine.Result:By using singly or mixed with thunberg fritillary bulb omni-alkaloids, there were obvious differences between these pharmacokinetic parameters of mesaconitine and aconitine: the elimination half-lifes and AUCs of two aconite alkaloids after used with thuberg fritillary bulb omni-alkaloids were longer or higher than those before matching, and the plasma clearances of two aconite alkaloids after used with thunberg fritillary bulb omni-alkaloids were lower than these before matching（P<0.05）.There were unsignificanted differences of hypaconitine used singly and used mixed with thunberg fritillary bulb omni-alkaloids（P>0.05）.Conclusion:Retention time and affection time of aconite alkaloids after used with thunberg fritillary bulb omni-alkaloids were longer than these before matching.The statement of “aconite counteracts fritillaria” is preliminarily validated by our pharmacokinetic studies.  
        关键词：mesaconitine;aconitine;hypaconitine;thunberg fritillary bulb omni-alkaloids;pharmacokinetics;HPLC   
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        发布时间：2024-09-23
      • Issue 5, Pages: 28-30(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.013
        摘要：目的观察清热祛湿胶囊主要药效作用。方法采用体内、外抑菌试验、10%蛋清所致足跖肿胀、二甲苯所致耳廊肿胀、热板法、醋酸所致化学致痛法、2,4二硝基苯酚所致发热模型、收集尿液等方法,观察清热祛湿胶囊的抑菌、抗炎、镇痛、解热和利尿作用。结果清热祛湿胶囊体外能抑制多种革兰阳性菌和多种革兰阴性菌的生长,体内给药能明显降低金黄色葡萄球菌和溶血型链球菌引起的感染模型小鼠的死亡率,能明显抑制大鼠足跖肿胀和小鼠耳廊肿胀,可明显降低2,4二硝基苯酚引起的家兔发热性体温,对正常大鼠有明显的利尿作用,并可延长小鼠痛反应时间,减少小鼠扭体次数。结论清热祛湿胶囊具有抑菌、抗炎、镇痛、解热、利尿作用。  
        关键词：清热祛湿胶囊;抑菌;抗炎;解热;镇痛;利尿   
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        发布时间：2024-09-23
      • Issue 5, Pages: 31-32(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.014
        摘要：目的对数种不同厂家的葛根素注射剂产品防治实验性大鼠缺血性脑损伤的作用进行比较。方法应用三氯化铁诱导大鼠大脑中动脉血栓栓塞模型,观察葛根素对大鼠的脑梗塞范围及脑含水量的影响。结果葛根素可以改善FeCl3诱导的大脑中动脉血栓栓塞模型大鼠的脑缺血症状,缩小其脑梗塞范围。结论葛根素对大鼠的实验性脑损伤具有良好的防治作用,各不同厂家的葛根素注射剂之间疗效未见明显差异。  
        关键词：葛根素;大脑中动脉;脑损伤   
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        发布时间：2024-09-23
      • WANG Dian-lei,LIU Qing-yun,GUO Li,XIA Jun
        Vol. 11, Issue 5, Pages: 33-35(2005)
        摘要：Objective:To study the effects of Shunxuekang(SXK) capsule on anti-thrombus.Method:We used the model rats of electro stimulation or venous thrombus to observe the effects of anti-thrombus of SXK capsule,and studied the probable mechanism of SXK capsule on blood coagulation,hemorrheology,platelet function.Results:SXK capsule could markedly prolong thrombosis time in rats,could markedly decrease the weight of venous thrombin,could markedly prolong clotting time in rats,could markedly decrease the whole blood viscosity,plasma relative viscosity,serene relative viscosity,and could markedly inhibit platelet aggregation induced by ADP.Conclusion:SXK capsule had markedly inhibitory effects on thrombus.  
          
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        发布时间：2024-09-23
      • WANG Dian-lei,LIU Qing-yun,GUO Li,XIA Jun
        Issue 5, Pages: 33-35(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.015
        摘要：Objective:To study the effects of Shunxuekang（SXK） capsule on anti-thrombus.Method:We used the model rats of electro stimulation or venous thrombus to observe the effects of anti-thrombus of SXK capsule,and studied the probable mechanism of SXK capsule on blood coagulation,hemorrheology,platelet function.Results:SXK capsule could markedly prolong thrombosis time in rats,could markedly decrease the weight of venous thrombin,could markedly prolong clotting time in rats,could markedly decrease the whole blood viscosity,plasma relative viscosity,serene relative viscosity,and could markedly inhibit platelet aggregation induced by ADP.Conclusion:SXK capsule had markedly inhibitory effects on thrombus.  
        关键词：Shunxuekang Capsule;Anti-thrombus;coagulation;hemcrrheology;platelet aggregation   
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        发布时间：2024-09-23
      • GAO Jun-wei1,DENG Xiu-ling1,MAO Hai-yan2,YUAN Bing-xiang1,WANG Yan1
        Vol. 11, Issue 5, Pages: 36-39(2005)
        摘要：Objective:To evaluate the efficacy of Divitamins Notonginseng and Cinnarizine Capsules(DNCC) on the global cerebral ischemia reperfuion injury.Methods:Ligating bilateral common carotid arteries and bilateral centrum arteries for ten minutes,and then reperfusing 72 hours induced the global cerebral ischemia reperfusion model.Observing the influence of DNCC on theelectroencephalogram(EEG),learning and memorizing abilities,brain index,water containing,and the number of the neuron alive.Results:DNCC can obviously reduce the decreasing scope of EEG,short recovering time of EEG after reperfusion,reduce learning and memorizing disturbance,brain index and water containing,and improve the living cells at hippocampi after reperfusion for 72 hours.Conclusion:DNCC can strongly protect the brains impaired by global cerebral ischemia reperfusion.  
        关键词：cerebral ischemia;reperfusion;electroencephalogram;learning and memorizing disturbance   
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        发布时间：2024-09-23
      • GAO Jun-wei1,DENG Xiu-ling1,MAO Hai-yan2,YUAN Bing-xiang1,WANG Yan1
        Issue 5, Pages: 36-39(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.016
        摘要：Objective:To evaluate the efficacy of Divitamins Notonginseng and Cinnarizine Capsules（DNCC） on the global cerebral ischemia reperfuion injury.Methods:Ligating bilateral common carotid arteries and bilateral centrum arteries for ten minutes,and then reperfusing 72 hours induced the global cerebral ischemia reperfusion model.Observing the influence of DNCC on theelectroencephalogram（EEG）,learning and memorizing abilities,brain index,water containing,and the number of the neuron alive.Results:DNCC can obviously reduce the decreasing scope of EEG,short recovering time of EEG after reperfusion,reduce learning and memorizing disturbance,brain index and water containing,and improve the living cells at hippocampi after reperfusion for 72 hours.Conclusion:DNCC can strongly protect the brains impaired by global cerebral ischemia reperfusion.  
        关键词：cerebral ischemia;reperfusion;electroencephalogram;learning and memorizing disturbance   
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      • Issue 5, Pages: 39-41(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.017
        摘要：目的探讨红景天注射液活血化瘀作用机理。方法观察红景天注射液对家兔血小板聚集率、大鼠体外血栓形成及血液黏度的影响。结果红景天注射液可明显降低花生四烯酸(AA,和胶原诱导的家兔血小板聚集率（P<0.05）,对二磷酸腺苷（ADP）诱导的家兔血小板聚集率有降低趋势;能显著缩短大鼠血栓长度（P<0.05）,减轻血栓湿重及干重（P<0.05～0.01）;降低中切速30S-1及低切速5S-1下的全血黏度（P<0.05）及血浆黏度。结论红景天注射液具有活血化瘀作用。  
        关键词：红景天注射液;血小板聚集;体外血栓;血液黏度   
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      • Issue 5, Pages: 42-44(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.018
        摘要：目的观察复方内鲁帕尔汤剂（CWPN）对免疫功能、发热、炎症的影响。方法测定血清溶血素生成,MTT法测T、B淋巴细胞的增殖,3HTdR法测定小鼠腹腔巨噬细胞的吞噬功能,用伤寒菌苗造成家兔体温上升研究降温作用,用小鼠腹腔毛细血管通透性法研究对炎症的影响。结果CWPN（4.0,8.0gkg）能够明显增强小鼠血清溶血素生成（P<0.05）,CWPN（0.01～10mgL）能够促进小鼠体外B淋巴细胞增殖,对T淋巴细胞无明显影响,能够增强小鼠腹腔巨噬细胞体外吞噬功能,能降低伤寒菌苗引起的家兔体温上升,CWPN（4.0,8.0gkg）能够抑制小鼠腹腔毛细血管炎性渗出。结论CWPN能够增强体液免疫和非特异性免疫功能,有较好的抗炎、解热功效。  
        关键词：复方内鲁帕尔;血清溶血素;淋巴细胞;免疫;抗炎;解热   
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      • Issue 5, Pages: 44-46(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.019
        摘要：目的研究黄芪丹参复方成分提高胎盘血供的分子机制,为临床有效防治胎盘血供不足所致妊娠并发症提供思路。方法提取分离中药黄芪、丹参复方有效成分,运用NOS（一氧化氮合酶）阻滞剂L精氨酸甲酯（LNAME）制作一氧化氮合成阻滞大鼠模型,免疫组化SABC法检测胎盘滋养细胞凋亡调控基因（bc12/Bax）蛋白表达变化。结果bc12蛋白质表达正常对照组、黄芪丹参复方组明显高于模型组（P<0.01）,Bax蛋白质表达正常对照组、黄芪丹参复方组、硝酸甘油组均明显低于一氧化氮合成阻滞模型组。结论黄芪丹参复方成分可能通过影响凋亡调控基因（bc12/Bax蛋白）表达变化,阻抑滋养细胞异常凋亡,防止胎盘浅表着床,有利于滋养细胞的植入,从而达到提高胎盘血液供应目的。  
        关键词：妊娠;高血压;并发症;凋亡;滋养细胞;蛋白质   
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      • Issue 5, Pages: 47-48(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.020
        摘要：目的研究藏药松石丸对实验性肝纤维化大鼠的影响。方法用40%CCl4皮下注射,每周2次,3～5mLkg,连续6周,引起大鼠肝纤维化模型。藏药松石丸自造模次日起灌胃给药,连续42天,测肝脏羟脯氨酸、肝胶原蛋白和血清中血脂。结果松石丸能降低肝纤维化动物的肝脏羟脯氨酸,肝胶原蛋白含量,并且降低血清中TCH、TG含量（与模型组比P<0.05或P<0.01>）。结论松石丸对大鼠实验性肝纤维化有显著疗效。  
        关键词：藏药;松石丸;四氯化碳;肝纤维化   
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      • Issue 5, Pages: 49-51(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.021
        摘要：目的动态比较益气活血法、化痰通腑法、潜阳熄风法三种中医治法对急性缺血性脑血管疾病的动物模型的疗效,探讨脑缺血急性期的证候属性及其演变规律。方法采用线栓法制作局灶性脑缺血再灌注大鼠模型,动态观察缺血2h再灌注24h、48h和72h,3种中医治法组方灌胃给药对一氧化氮（NO）、一氧化氮合酶（NOS）、超氧化物歧化酶（SOD）、丙二醛（MDA）和神经细胞凋亡率的变化。结果（1）脑缺血2h再灌注24h、48h、72h,模型组指标较假手术组均有显著病理性改变（2）缺血2h再灌注24h,各治疗组与模型组相比均有显著改善。组间比较,增强SOD活性潜阳熄风组最优。（3）缺血2h再灌注48h,各治疗组与模型组相比均有显著改善。组间比较,降低脑匀浆NOS益气活血组最优。（4）缺血2h再灌注72h,各治疗组均能显著改善SOD/MDA和神经细胞凋亡的病理改变。组间比较,降低脑匀浆MDA化痰通腑组最优;降低脑匀浆NO含量和NOS活性,益气活血组最优。结论潜阳熄风法,在缺血2h再灌注24h,提高SOD活性;益气活血法,在缺血2h再灌注48h及72h,降低NO含量和NOS活性;化痰通腑法,在缺血2h再灌注72h,清除过量MDA。  
        关键词：脑缺血—再灌注损伤;益气活血法;化痰通腑法;潜阳熄风法;动态比较;演变规律   
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      • XIAO Wen-Hui1,WANG Xiang-Gui2
        Vol. 11, Issue 5, Pages: 52-54(2005)
        摘要：Objectives:To study the anti-dysmenorrhea effect of Tongjing Plaste.Methods:Four experimental observations were carried out twisting body reaction of rat,the effect on the concentration of PGE2 of rat models of dysmenorrhea,the effect on extracorporeal uterine smooth muscle of rabbits,the effect on extracorporeal uterine smooth muscle of rabbits which sensitized by oxytocin.Results:Tongjing Plaster evidently inhibited twisting body reaction of rat models of dysmenorrhea,reduced the frequency and extent of contraction in normal extracorporeal uterine smooth muscle and in uterine muscle sensitized by oxytocin,and decreased the level of PGE2 Conclusion:Tongjing Plaster can alleviate experimental dysmenorrhea.  
        关键词：Tongjing Plaster;dysmenorrhea;Experiment on animal;Pharmacology medicine   
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      • XIAO Wen-Hui1,WANG Xiang-Gui+2
        Issue 5, Pages: 52-54(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.022
        摘要：Objectives:To study the anti-dysmenorrhea effect of Tongjing Plaste.Methods:Four experimental observations were carried out twisting body reaction of rat,the effect on the concentration of PGE2 of rat models of dysmenorrhea,the effect on extracorporeal uterine smooth muscle of rabbits,the effect on extracorporeal uterine smooth muscle of rabbits which sensitized by oxytocin.Results:Tongjing Plaster evidently inhibited twisting body reaction of rat models of dysmenorrhea,reduced the frequency and extent of contraction in normal extracorporeal uterine smooth muscle and in uterine muscle sensitized by oxytocin,and decreased the level of PGE2 Conclusion:Tongjing Plaster can alleviate experimental dysmenorrhea.  
          
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      • ZHANG Qi-chun1,WANG Qiu-juan1,KOU Jun-ping2,ZHU Dan-ni2,YAN Yong-qing2,YU Bo-yang2
        Vol. 11, Issue 5, Pages: 55-58(2005)
        摘要：Objective:Investigating the protective effect of CSF of rat administrating FBD orally on various injured PC12 cells models to indicate the mechanism of FBD therapying dementia.Methods:Four injured PC12 models induced by hydrogen peroxide,hydrosulfurous sodium,glutamate and potassium chloride accordingly were used to assay the effect of CSF sample on PC12 cells.Results:It was found that the rat CSF sample at different time points from 0.5h to 2.5h after taking FBD repetitiously (1.62 g crude herb·kg-1) could obviously increase the viability of PC12 cells injured by hydrogen peroxide,which peaked at 1.5h.The rat CSF containing FBD of 1.5h could also protect PC12 cells injured by hydrosulfurous sodium、glutamate and potassium chloride,and the protection rate increases with the concentration of CSF containing FBD.Conclusion:CSF of rat administrating FBD orally could protect PC12 cells,possibly by antagnizing the overload of calcium,oxidant injury of free radicals and excitatory nerotoxicity of glutamate.  
        关键词：Optimized Dang Gui Shao Yao San(FBD);cerebrospinal fluids;PC12 cell;overload of calcium   
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      • ZHANG Qi-chun1,WANG Qiu-juan 1,KOU Jun-ping2,ZHU Dan-ni2,YAN Yong-qing2,YU Bo-yang2
        Issue 5, Pages: 55-58(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.023
        摘要：Objective:Investigating the protective effect of CSF of rat administrating FBD orally on various injured PC12 cells models to indicate the mechanism of FBD therapying dementia.Methods:Four injured PC12 models induced by hydrogen peroxide,hydrosulfurous sodium,glutamate and potassium chloride accordingly were used to assay the effect of CSF sample on PC12 cells.Results:It was found that the rat CSF sample at different time points from 0.5h to 2.5h after taking FBD repetitiously （1.62g crude herb·kg -1 ） could obviously increase the viability of PC12 cells injured by hydrogen peroxide,which peaked at 1.5h.The rat CSF containing FBD of 1.5h could also protect PC12 cells injured by hydrosulfurous sodium、glutamate and potassium chloride,and the protection rate increases with the concentration of CSF containing FBD.Conclusion:CSF of rat administrating FBD orally could protect PC12 cells,possibly by antagnizing the overload of calcium,oxidant injury of free radicals and excitatory nerotoxicity of glutamate.  
        关键词：Optimized Dang Gui Shao Yao San（FBD）;cerebrospinal fluids;PC12 cell;overload of calcium   
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      • LIU Min 1,2,TAN Yu-qing1,JIANG Nan1,HUANG Jing-yao2,HUO Hai-ru1,LI Lan-fang1,GUO Shu-ying1
        Vol. 11, Issue 5, Pages: 59-61(2005)
        摘要：Objective:To investigate the effects of different combinations of four components in Guizhi decoction on secretion of prostaglandin E2(PGE2) by interleukin-1β(IL-1β) stimulation in rats cerebral microvascular endothelial cell(rCMEC).These four components are among the extracted fractions of Guizhi Decoction.Methods:The amounts of PGE2 were measured by radioimmunoassay method.Results:Twenty of the combinations significantly decreased the excessive secretion of PGE2 induced by IL-1β in the supernatant of rCMEC.The results had the statistical signification.Conclusion:The above mentioned combinations might be the substance foundation of the Fr.A of the decoction in its dual-directional thermoregulation.  
        关键词：Guizhi decoction;IL-1β;Rats cerebral microvascular endothelial cell(rCMEC);PGE2   
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      • LIU Min 1,2,TAN Yu-qing1,JIANG Nan1,HUANG Jing-yao2,HUO Hai-ru1,LI Lan-fang1
        Issue 5, Pages: 59-61(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.024
        摘要：Objective:To investigate the effects of different combinations of four components in Guizhi decoction on secretion of prostaglandin E2（PGE2） by interleukin-1β（IL-1β） stimulation in rats cerebral microvascular endothelial cell（rCMEC）.These four components are among the extracted fractions of Guizhi Decoction.Methods:The amounts of PGE2 were measured by radioimmunoassay method.Results:Twenty of the combinations significantly decreased the excessive secretion of PGE2 induced by IL-1β in the supernatant of rCMEC.The results had the statistical signification.Conclusion:The above mentioned combinations might be the substance foundation of the Fr.A of the decoction in its dual-directional thermoregulation.  
        关键词：Guizhi decoction;IL-1β;Rats cerebral microvascular endothelial cell（rCMEC）;PGE2   
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      • ZHAO Bao-sheng1,DONG Shu-yun2,HUO Hai-ru1,JIANG Ting-liang1
        Vol. 11, Issue 5, Pages: 62-66(2005)
        摘要：Objective:To introduce Some establishing methods to Non-insulin-dependent diabetes mellitus(NIDDM) animal model.Methods:The established methods and techniques for the NIDDM animal model are summarized.Results:The NIDDM animal model's establishing methods can meanly be divided into four aspects:spontaneous NIDDM model, experimental NIDDM model, gene knock-out NIDDM model and anti-insulin cell model for NIDDM.Conclusion:There are advantages and disadvantages in the four aspects to establish the NIDDM animal model.The experimental NIDDM model becomes the most important and commonly used animal model to research NIDDM due to the low-cost, better maneuverability and the similar pathological process with that of human being.  
        关键词：Non-insulin-dependent diabetes mellitus;animal model;Establishing methods   
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      • ZHAO Bao-sheng1,DONG Shu-yun2,HUO Hai-ru1,JIANG Ting-liang1
        Issue 5, Pages: 62-66(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.025
        摘要：Objective:To introduce Some establishing methods to Non-insulin-dependent diabetes mellitus（NIDDM） animal model.Methods:The established methods and techniques for the NIDDM animal model are summarized.Results:The NIDDM animal model’s establishing methods can meanly be divided into four aspects:spontaneous NIDDM model, experimental NIDDM model, gene knock-out NIDDM model and anti-insulin cell model for NIDDM.Conclusion:There are advantages and disadvantages in the four aspects to establish the NIDDM animal model.The experimental NIDDM model becomes the most important and commonly used animal model to research NIDDM due to the low-cost, better maneuverability and the similar pathological process with that of human being.  
        关键词：Non-insulin-dependent diabetes mellitus;animal model;Establishing methods   
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      • WANG Yong-ze,YANG Hong-zhi,Dai Min,LI Yong-wei,GUAN Wei-bing,YANG Yue-wu
        Vol. 11, Issue 5, Pages: 67-69(2005)
        摘要：To examine the impact of Jianpihuashisanyu Fang and Xuezhikang recipe on the serum lipids and liver function in patients with chronic hepatitis B (CHB) and fatty liver.Method:107 patients with chronic hepatitis B and fatty liver were treated for 8 weeks and randomly divided into two groups:the research group has 62 patients treated by Jianpihuashisanyu Fang and Xuezhikang recipes (bid);the control group has 45 patients treated by Xuezhikang recipes(bid).The serial indices of serum lipids(including TC、TG、HDL-C、LDL-C、ApoA1、ApoB、LP(a)),and the liver function (including ALT、AST、CHE)were tested.Results The level of TC、LDL-C and ApoB were samely decreased in two groups(P>0.05),but the level of TC in research group was significantly lower than that of the control(P<0.05),the level of HCL-C and Apo A1 in research group was significantly higher than that of the control (P<0.05).In research group,the ALT、AST and CHE were significantly decreased to the normal level(P<0.01).Conclusion:Effect of the treatment of Jianpihuashisanyu Fang and Xuezhikang recipe on CHB with fatty liver is significantly better than treated by Xuezhikang recipe only.  
        关键词：Jianpihuashisanyu Fang;CHB with fatty liver;serum lipid;liver function   
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      • WANG Yong-ze,YANG Hong-zhi,DAI Min,LI Yong-wei,GUAN Wei-bing,YANG Yue-wu
        Issue 5, Pages: 67-69(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.026
        摘要：To examine the impact of Jianpihuashisanyu Fang and Xuezhikang recipe on the serum lipids and liver function in patients with chronic hepatitis B （CHB） and fatty liver.Method:107 patients with chronic hepatitis B and fatty liver were treated for 8 weeks and randomly divided into two groups:the research group has 62 patients treated by Jianpihuashisanyu Fang and Xuezhikang recipes （bid）;the control group has 45 patients treated by Xuezhikang recipes（bid）.The serial indices of serum lipids（including TC、TG、HDL-C、LDL-C、ApoA1、ApoB、LP（a））,and the liver function （including ALT、AST、CHE）were tested.Results The level of TC、LDL-C and ApoB were samely decreased in two groups（P>0.05）,but the level of TC in research group was significantly lower than that of the control（P<0.05）,the level of HCL-C and Apo A1 in research group was significantly higher than that of the control （P<0.05）.In research group,the ALT、AST and CHE were significantly decreased to the normal level（P<0.01）.Conclusion:Effect of the treatment of Jianpihuashisanyu Fang and Xuezhikang recipe on CHB with fatty liver is significantly better than treated by Xuezhikang recipe only.  
        关键词：Jianpihuashisanyu Fang;CHB with fatty liver;serum lipid;">   
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      • Vol. 0, Issue 5, Pages: 70(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.027
          
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      • Vol. 0, Issue 5, Pages: 70-71(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.028
          
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      • Vol. 0, Issue 5, Pages: 71-72(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.029
          
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      • Vol. 0, Issue 5, Pages: 72-73(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.030
          
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      • Vol. 0, Issue 5, Pages: 73(2005) DOI: 10.13422/j.cnki.syfjx.2005.05.031
          
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