最新刊期
卷 ， 期 1 ， 2008
- 摘要：Objective:To develop an HPLC method for determination of baicalin in Qianjin Baoyun Pills.Methods:The chromatographic column was Agilent Hypersil ODS（4.0 mm×250 mm,5 μm）.The mobile phase was methanol-0.4% phosphoric acid（43∶57）.The flow rate was 1 mL·min-1 and the detection wavelength was 280 nm.Results:The linear range of baicalin was within（7.23～115.68）μg·mL-1（r=（0.999 9））,the average recovery for baicalin was 100.6%,RSD was 0.58%.Conclusion:This method is simple,accurate and could be used for quality control of Qianjin Baoyun Pills.关键词：Qianjin Baoyun Pills;baicalin;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To develop a HPLC method for determination of indirubin in Shengxuexiaoban Tablets.Methods:Kromasil C18（4.6 mm×250 mm,5 μm） column was used with acetonitrile-water（53∶47） as mobile phase,and the detection wavelength was set at 290 nm.Results: The linear range was（0.3028～3.028）μg for indirubin（r=（0.999 7））,the average recovery was 97.4% and RSD was 0.7%（n=6）.Conclusions:The method is simple,reliable,and accurate for the determination of indirubin in this preparation.关键词：Shengxuexiaoban Tablets;indirubin;determination;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To develop a RP-HPLC method for determination of berberin in Kangpenyan Jiaonang.Methods:Berberin was separated completely on an C18 column,using a mixture of methanol,acetonitrile and water（40∶21∶39）（containing 0.8 g Sodium dodecyl sulphonate and 0.5 g tartaric acid）as mobile phase,with UV detection at 346 nm.Results:The linear range of berberin was in（0.083～0.747）μg,the recovery rate was 96.34%,RSD=0.98%.Conclusions:The method is very stable,accurate,and repeatable.It can be used for quality control of Kangpenyan Jiaonang.关键词：RP-HPLC;Kangpenyan Jiaonang;berberin;content determination
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发布时间：2024-09-23 - 摘要：Objective:To develop a RP-HPLC method for determination of kaempferide in Fuyang Tablets.Methods:Diamonsil C18 column（4.6 mm×250 mm,5 μm）was used,methanol-0.4% phosphoric acid solution（58∶42）as the mobile phase,flow rate at 1.0 mL·min-1,and the detection wavelength was 367 nm.Results: The calibration curves were linear between（0.041～0.41）μg,The average recovery and the relative standard deviations were 99.1% and1.1%.Conclusions: The method is simple,accurate,repeatable and could be used for determination of kaempferide in the preparation.关键词：RP-HPLC;Fuyang Tablets;kaempferide;Assay
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发布时间：2024-09-23 - 摘要：目的:改进益肾养元合剂中何首乌、金樱子、补肾脂药材的薄层色谱鉴别方法。方法:采用薄层色谱进行鉴别分析。结果:增加了益肾养元合剂中何首乌、金樱子、补骨脂药材的薄层色谱鉴别。结论:薄层色谱鉴别方法快速简便、稳定,专属性好,为完善益肾养元合剂的鉴别标准提供依据。关键词：薄层色谱;质量;益肾养元合剂
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发布时间：2024-09-23 - 摘要：目的:用HPLC法测定加味四逆颗粒中柴胡皂苷a的含量。方法:样品经甲醇超声提取,使用C18色谱柱,乙腈-水（37∶63）为流动相,检测波长208 nm,流速1 mL·min-1,柱温35℃。结果:柴胡皂苷a线性范围（0.21.6）μg,相关系数r=0.9998,平均回收率99.9%。结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可用于加味四逆颗粒中柴胡皂苷a的含量测定。关键词：加味四逆颗粒;柴胡皂苷A;高效液相色谱法
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发布时间：2024-09-23 - 摘要：Objective:To establishthe quality control of Arctium lappa L..Methods:C18 column,mobile phase was Acetonitrile-0.4% phosphoric acid-water（8∶92）.The detection wavelength was at 327 nm.Results:The linearity was good within a range of（0.088～0.704）μg（r=（0.999 9））.The average recovery was 98.34%.Conclusions:Chlorogenic acid is a suitable component for the quality control of Arctium lappa L..关键词：Arctium lappa L.;chlorogenic acid;HPLC
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发布时间：2024-09-23 - 摘要：目的:建立连蒲双清片质量标准。方法:以咖啡酸为对照品,以蒲公英对照药材进行薄层色谱鉴别;采用高效液相色谱法测定制剂中盐酸小檗碱的含量,色谱条件:C18柱,以磷酸盐缓冲液[0.05 mol·L-1磷酸二氢钾和0.05 mol·L-1庚烷磺酸钠（1∶1）,含0.2%三乙胺,并用磷酸调节pH值至3.0]-乙腈（60∶40）为流动相,检测波长为263 nm。结果:盐酸小檗碱的回收率为100.41,RSD为0.12%,薄层图斑点清晰,阴性对照无干扰。结论:方法简便准确,重复性好,可用于连蒲双清片的质量控制。关键词：连蒲双清片;盐酸小檗碱;蒲公英;高效液相色谱法;薄层色谱法;咖啡酸
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发布时间：2024-09-23 - 摘要：Objective:To select the Pressure sensitive adhere used in the transdermal study of EBM.Methods:Transdermal speed,constant tackness,sustained adhesion and peeling strength were observed,the improved Franz cell and Skin of abdomen of SD mice were used to study the selection of pressure sensitive adhere.Results:The optimum PSA was DOW CORNING MD-0607.Coclusions:The result indicateed that the DOW CORNING MD-0607 was the optimum PSA used the transdermal study of EBM.关键词：artemisinin derivate;transdermal study;pressure sensitive adhesive
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发布时间：2024-09-23 - 摘要：目的:研究大孔树脂纯化葛根丹参药对水溶性有效成分的工艺条件及参数。方法:以有效成分葛根素和丹酚酸B的洗脱率和吸附量为评价指标,筛选大孔树脂纯化葛根丹参有效成分的最佳工艺。结果:选用D-101-A型大孔树脂,除杂洗脱用水量为3 BV,洗脱剂乙醇浓度为60%,乙醇用量为3 BV。结论:通过大孔吸附树脂纯化后,葛根丹参药对水溶性成分得到有效纯化。关键词：葛根素;丹酚酸B;大孔树脂
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发布时间：2024-09-23 -
Studise on Preparation Technology of Mosla Chinensis Essential Oil-β-cyclodextrin Inclusion Compound
摘要：Objective:To study the preparation technology of Mosla chinensis Essential oil-β-cyclodextrin（β-CD）inclusion compound.Methods:It was studied with Orthogonal design on the extraction of Essential oil of Mosla chinensis and β-cyclodextrin inclusion compound.The utilization ratio of Essential oil,and the content of carvacrol and thymol were used as criteria in the test.Results:The optimum preparation technology for the inclusion compound of Mosla chinensis Essential oil-β-CD was using the collid mill to grind instantaneously and inclusion rapidly.The best condition were as follows: The inclusion times was 3,the temperature for inclusion was 30℃,the ratio among the essential oils,β-CD and water was 1∶6∶18.Conclusions:This complex prepared on the condition aforementioned is simple and stable.It can be used for the production in industry.关键词：essential oil of mosla chinensis;grinding instantaneously and inclusion rapidly by the colloid mill（β-CD） preparation technology;carvacrol and thymol- 0
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发布时间：2024-09-23 -
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- 摘要：Objective:To establish a revered phase HPLC method for the study of pharmacokinetics of loganin in Fructus Corni after a single oral administration.Methods:The Hypersil C18（4.6 mm×250 mm,5 μm） was used as the analytical column and the mobile phase consisted of acetonitrile-methanol-0.1% phosphoric acid（10∶5∶85） with the flow rate at 1.0 mL·min-1.The UV detection was set at 236 nm.The pharmacokinetic parameters of loganin were recorded.Results:After orally taken Fructus Corni,the plasma concentration-time course in rats fitted well to one-compartment model with the 1’st order absorption and with the following pharmacokinetic parameters:Ka=0.06 min-1,Ke=0.013 min,T（peak）=33.90 min,t1/2ka=12.47 min,t1/2ke=52.45 min.Conclusions:The HPLC method to determine plasma concentration of loganin and pharamacokinetic parameters in rats is feasible.关键词：Fructus Corni;loganin;pharmacokinetics;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Zhinao Capsule on behavior and the content of Nitric Oxide（NO）,Endothelin（ET）,Calcitonin gene related peptide（CGRP） in brain tissue of rats with vascular dementia.Methods:After intraperitoneal injection of low dose of sodium nitroprusside,animal models of vascular dementia were established by clamping common carotid arteries repeatedly.Their learning and memory ability and the content of NO,ET,CGRP of brain tissue were detected.Results:Compared with model group,three doses of Zhinao Capsule treated group could improve the ability of learning and memory obviously;The middle and high dose of Zhinao Capsule treated group,piracetam treated group and nimodipine treated group could significantly decrease the content of NO;Zhinao Capsule treated group,piracetam treated group and nimodipine treated group could significantly decrease the content of ET and improve the content of CGRP.High dose of Zhinao Capsule could decrease the content of ET and the content of CGRP,which was more significant than the piracetam treated group and the middle and low dose of Zhinao Capsule treated group.Conclusions:Zhinao Capsule could improve the learning and memory ability in rats with vascular dementia,which may be related to decreasing the content of NO and ET and increasing the content of CGRP of brain tissue in rats with vascular dementia.关键词：vascular dementia;Zhinao capsule;learning-memory abitity;nitric oxide;endothelin;calcitonin gene related peptide
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发布时间：2024-09-23 - 摘要：Objective:To study effects of Qingfeixiaoke granule on antitussive,expectorant,antiasthmatic and anti-bacteria,anti-virus,anti-inflammatory.Methods:The following experiments were carried out: cough induced by ammonia, removing phlegm with phenol red,in vivo and in vitro tests for relieving asthma.The effects of the drug on relieving cough,removing phlegm and relieving asthma were investigated;The in-vitro anti-bacteria,and the mouse model infected by pathogenic bacteria,The mouse model of ear edema caused by xylene and the nat midle of tampon granuloma were established to evaluate the anti-bacteria,anti-virus,anti-inflammatory effects.Results:The antitussive expectorant and antiasthmatic experiments of Qingfeixiaoke granule showed that it can restrain mice to cough by ammonia,increase phenol red secretion of mice in bronchus,release asthma induced by histamine phosphate-acetylcholine in guinea pigs,increased latent period of breathe with difficulty and induced asthma.the Anti-bacteria,anti-virus and anti-inflammatory experiments showed that Qingfeixiaoke granule had strong an effect on inhibiting or drowned activity pneumonia streptococcus、α/β-hemolytic streptococcus,staphylococcus aureus and had infirm an effect on inhibiting bacillus coli and bacillus pyocyaneus,the death rate of infected by pneumonia streptococcus in mice could reduce and could prolong the mean survival days.Qingfeixiaoke granule indicated no toxicity to MDCK cells at 12.5 mg·mL-1,and could effectively inhibit influenza A1 virus at 0.78 mg·mL-1,could markedly debase indexes of lung of mouse pneumonitis which was elicited by influenza virus;Qingfeixiaoke granule could significantly suppress ear swell induced by xylene in mice and granuloma proliferation.Conclusions:Qingfeixiaoke granule has significant effects on relieving cough,removing phlegm,relieving asthma,also shown effects on anti-bacteria,anti-virus,anti-inflammatory.关键词：Qingfeixiaoke Granule;antitussive;expectorant;antiasthmatic;anti-bacteria;anti-virus;anti-inflammatory
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发布时间：2024-09-23 - 摘要：Objective:The protective effects of total flavones of Pollen Typhae on acute myocardial ischemia were investigated in dogs.Methods:Acute myocardial ischemia model was made by ligation of left anterior descending artery in the anesthetized open chest dogs.The effects of total flavones of Pollen Typhae on epicardium electrocardiogram（EECG）and myocardial infarction square（MIS） were measured,and effects on enzymology in serm were also determined.Results:Total flavones of Pollen Typhae can reduce the degree and scope of myocardial ischemia（∑-ST and N-ST）,and decrease myocardial infarction square.Moreover,it also can reduce FFA and LPO content in serum,and improve SOD and GSH-Px activity in acute myocardial dogs.Conclusions:Total flavones of Pollen Typhae have the protective effects on acute myocardial ischemia dogs.关键词：total flavones of pollen typhae;myocardial ischemia;epicardium electrocardiogram;enzymology in serum
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发布时间：2024-09-23 - 摘要：Objective: To observe the influences of Changan Granule on immune function in spleen deficiency mice and Methods: Using the model of spleen deficiency of mice induced by Radix et Rhizoma Rhei,effects of Changan Granule were studied through observing the influences on survival rate and spleen index of mice.Effects of Changan Granule on immune function were studied by analyzing the clearance rate of carbon particle（K） and the value of HC50.Results: The Changan Granule can increase the spleen index,decrease the death rate within 3 hours,and increase the clearance rate of carbon particle（K） and the value of HC50（P<0.05）.Conclusions: Changan Granule is effective to cure splenic asthenia,and can promote the phagocytic function of mononuclear macrophage and reinforce humoral immunity.关键词：Changan Granule;irritable bowel syndrome;model of spleen deficiency;immune function
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发布时间：2024-09-23 - 摘要：Objective:To study the effection of Tangnai-Kang conction for insulin resistance and expression of Phosphatidylinositol 3 Kinase、Glucose Transport 4 messenger ribonucleic acid（mRNA）.Methods:Specific pathogen free（SPF） KKAy mice 40 were divided randomly according to plasma glucose level into Models,TNK-low dosage,TNK-high dosage,Pioglitazone and normal C57BL/6J mice（normal control group）.60 days later,all animals were tested Fasting plasma glucose（PFG）,Fasting insulin level for caculating Insulin Sensitivity Index（ISI）,Total Cholesterol（TC）,Total triacylglycerol（TG）,High-density Lipoprotein Cholesterol（HDL-C）,Low-density Lipoprotein Cholesterol（LDL-C）,Floating fatty acid（FFA）,Tumor necrosis factor-α（TNF-α） and pathological examination for the islet.The muscle and adipose expressio of PI-3K and GluT4 mRNA by reverse transcriptase PCR（RT-PCR） were determined.Results:Compared with Model groups,FPG、TNF-α levels were decreased significantly for TNK-high dosage（P<0.05）,the expression of PI-3K and GluT4 mRNA were all up-regulated（P<0.05 or P<0.01）,the recipe could protect β-cells in some degree and postponting β-cells failure in islet,displayed decreasing tendency to plasma lipocy and FFA levels.Conclusions:One of the mechanism of Tangnai-Kang improving insulin resistance might be up-regulated the expression of PI-3K mRNA in post-receptor of insulin signal-transduction roads,thereby increased GluT4 translocation.关键词：Tangnai-Kang;insulin signal-transduction;KKAy mice;type 2 diabetes mellitus
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Jiawei Sini Powder on monoamine transmitters of Hypothalamus of rat chronic stress model.Methods:Male Sprague-Dauley rats were randomly allocated to five groups as follows:control,model,fluoxetine,Jiawei Sini Powder with small dose and largt dose.The model was produced by sepration and chronic unpredictable mild stress.The behavior was detected by open-field test.HPLC-EC was used to study the changes of monoamine transmitters of Hypothalamus.Results:The results showed that NE level of model group is higher,and 5-HIAA and 5-HT are lower.Fluoxetine and Jiawei Sini Powder decreased the NE level and increased 5-HIAA and 5-HT.Conclusions:The data suggested that dysfanction of monoamine nerve of hypothalamus in rat chronic stress model is related with increased NE and decreased 5-HT,regulation for which may explain the mechanism of Jiawei Sini Powder.关键词：Jiawei Sini Powder;chronic stress;rats;monoamine transmitter;Hypothalamus
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发布时间：2024-09-23 - 摘要：目的:观察复方血竭对溃疡性结肠炎（UC）实验大鼠的治疗作用及其细胞因子（CK）的调节作用。方法:将动物随机分为复方血竭组、柳氮磺胺吡啶（SASP）组、模型组、正常对照组,除正常对照组外其余3组动物分别用2,4-二硝基氯苯（DNCB）建立大鼠溃疡性结肠炎模型,给予相应治疗,镜下观察其结肠黏膜病理学变化,并测定结肠组织IL-1β,IL-4,IL-6,IL-10的水平。结果:模型组大鼠结肠黏膜糜烂、炎细胞浸润与溃疡形成情况严重,其它各组分别有不同程度的炎症修复、溃疡愈合,其中尤其以复方血竭组最为明显。复方血竭组的IL-1β,IL-6水平较模型组明显降低,IL-4,IL-10的水平明显升高。结论:复方血竭抑制了UC大鼠IL-1β,IL-6,上调了IL-4和IL-10,纠正异常的免疫功能,从而有利于炎症的消除及组织的修复。关键词：复方血竭;溃疡性结肠炎;细胞因子
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Gubisan on mouse experimental ostarthritis.Methods: The experimental ostarthritis model was made by cutting off medial collateral ligament and medial aponeurosis of two hind limbs of mice.Gubisan was given to mice to observe the histopathology of cartilage and synovium,and the content of nitric oxide（NO） and superoxide dismutase（SOD） and malondialdehyde（MDA） in sera. Results: Gubisan(3.66,7.32 g·kg-1·d-1) can significantly inhibit cartilage damage,synovitis and synivium hyperplasia,suppress the lost of collagen Ⅱ in cartilage,reduce the activity of NO and MDA and increase the cevel of SOD in sera.Conclusions: Gubisan can prevent and cure experimental ostarthritis,which is related to the decrease of NO and MDA and activation of SOD.关键词：Gubisan;ostarthritis in mice;NO;SOD;MDA
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发布时间：2024-09-23 - 摘要：Objective:To explore a new method for the study of compatibility attenuation in TCM, and to investigate the effect of Glycyrrhiza uralensis saponins（GS）on the reduction of the acute toxicity of platycodins.Methods:The death rate of the mice was used to evaluate the acute toxicity induced by platycodins in the condition of pre-treatment with GS and no GS.Furthermore,the attenuation effects of different concentrations of GS were investigated by LD50 method.Results:Pre-treatment of different concentrations of GS can reduce obviously the death rate of the mice,elevate the 95% confidence level of the LD50 value,prolong the survival time of the mice.Conclusions:Pre-oral administration of GS can partly alleviate the toxicity of platycodins and reduce the death induced by platycodins.关键词：glycyrrhiza uralensis saponins;platycodins;compatibility attenuation;LD50
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of lowering blood glucose on experimental animals by Lingzhijiangtang capsule.Methods and Resalts:The results showed that this capsule has obvious decresing effect on increased blood glucose induced by alloxan.Conclusions:Lingzhijiangtang capsule may be effective on type Ⅱ diabetes.关键词：Lingzhijiangtang Capsule;diabetes;effect of lowering blood glucose
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发布时间：2024-09-23 - 摘要：目的:观察疏肝和胃方汤剂治疗胃食管返流病的有效性和安全性。方法:选择胃食管返流病的患者78例,随机分为治疗组42例和对照组36例。治疗组服用疏肝和胃煎剂（100 mL/袋）,对照组口服西沙比利（5 mg/胶囊）,15 d为1个疗程,服用2个疗程。治疗后进行症候评分和胃镜观察。结果:两组治疗前后血、尿、大便及心、肝、肾功能均无异常;治疗组治疗后胃脘不适和泛酸、烧心、口苦、舌苔腻等症状体征的评分明显低于治疗前及对照组（P<0.050.01）;治疗后,两组胃镜检测胃黏膜充血糜烂明显减轻,与对照组比较,总有效率有显著差异（P<0.05）。结论:疏肝和胃方对胃食管返流病有较好的疗效,安全性好。关键词：胃食管返流病;中药疗法;肝胃不和证;汤剂
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发布时间：2024-09-23 - 摘要：目的:观察灭幽汤对脾胃湿热型幽门螺杆菌（Hp）相关性胃炎的疗效。方法:60例Hp感染慢性胃炎患者随机分为2组,治疗组32例给予灭幽汤治疗,对照组28例给予西药治疗。服药2周后于第4周复查胃镜。结果:两组在减轻胃黏膜炎症程度、根除Hp方面均有显著作用（P<0.05）,且炎症程度与Hp呈高度正相关,但在改善症状、提高疗效方面,治疗组优于对照组（P<0.05）。结论:灭幽汤能直接作用Hp,减少炎症细胞浸润,有效治疗湿热型Hp感染慢性浅表性胃炎。关键词：灭幽汤;慢性胃炎;幽门螺杆菌
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发布时间：2024-09-23 - 摘要：目的:分析2004年4月2004年9月花粉症患者就诊人次与北京市同期空气花粉浓度的关系。方法:选择2004年北京世纪坛医院变态反应科就诊457例花粉症患者,并与同期国家气象中心提供的2004年4月2004年9月的花粉监测数据进行相关性分析。结果:花粉症的发病季节基本与北京市城区气传花粉高峰期吻合,花粉症与患者就诊人次与花粉总量、秋季致敏花粉及致敏花粉总量为高相关。结论:秋季花粉仍为北京地区的主要致敏花粉;患者发病与花粉总量及致敏花粉的数量有关。关键词：花粉症;变应原;气传花粉
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发布时间：2024-09-23 - 摘要：Objective:To summarize the recent research state on therapy of vascular dementia by traditional chinese medicine.Methods:Literature about clinical treatment research on vascular dementia by traditional chinese medicine in recent years was reviewed.Results:Based on differentiation of symptom-complex and using some prescriptions of traditional chinese medicine,traditional chinese medicine had good therapeutic effects in the aspects of improving the intellectual level and social adaptation ability for the patients with vascular dementia.Conclusions:Traditional Chinese medicine has its advantage in the treatment on vascular dementia.关键词：vascular dementia;compound prescription;therapy
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发布时间：2024-09-23 - 摘要：Objective:To review advances of pharmacokinetics study of Semen Strychni and its dosage forms.Methods:Sum up the methods for contents determination of strychnine and brucine in biological samples.Review advances of pharmacokinetic study of Semen Strychni and its dosage forms.Results:The concentration in biological samples was detected by spectrophotometry,TLC,HPLC or HPLC-MS. The highest distribution of brucine is liver and kidney,and conformed to two compartment model.Strychnine distributes higher in heart,and it eliminated in linear dynamics,and conformed to one compartment model.Conclusions:It remains to be studied whether Strychnine cumulated when use chronically,and the pharmacokinetics properties with repeated does should be further studied.关键词：Semen Strychni;strychnine;brucine;pharmacokinetics
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发布时间：2024-09-23 -
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