最新刊期
      卷 15 ， 期 6 ， 2009
      • WANG Chun,LV Shu-yi,SONG Zhi-qian,WANG Chao,LIU Zhen-li
        Vol. 15, Issue 6, Pages: 1-3(2009)
        摘要：Objective:To develop a RRLC method for the content determination of flavonoids in Fructus Aurantii Immaturus. Method: RRLC was applied with a Zobax Extend-C18 colunmn by methanol-2% ethanoic acid (33∶77) as the mobile phase.The flow rate was 1.0 mL·min-1 .The column temperature was 30℃ and the UV detection wavelength was 283 nm. Result: In the 27 batches samples, 19 batches contained hesperidin, narirutin, neohesperidin and naringin, and they can divide into two groups by the ratio of flavonoids: in one group the content of hesperidin and narirutin were higher, and in another group the content of neohesperidin and naringin were higher; The other 8 batches only contain hesperidin and narirutin, and the content of hesperidin was higher. The content range of 4 kinds of main flavonoids in each sample were from 12.4% to 38.07%. Conclusion: The method is stable and reliable, and the analysis time of RRLC method was one-third of that of the HPLC method. The types and the content ratios of flavonoids in Fructus Aurantii Immaturus were different.  
        关键词：RRLC;Fructus Aurantii Immaturus;flavonoid;content   
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        发布时间：2024-01-04
      • LI Rao-rao1,WANG Cai-fang1,2,HUANG Lan-lan1,3,ZHONG Li-qun4,YUAN Si-tong1
        Vol. 15, Issue 6, Pages: 4-6(2009)
        摘要：Objective:To analyze the effect on HPLC fingerprint of Flos Genkwa which samples prepared by macroporous resin. Method: The fingerprints of 17 batches of Flos Genkwa were determined. The samples were extracted with methanol, then solvent were distilled off underreduced pressure. The residue were solved by water. Macroporous resin column chromatography was used for separating the water solution. Three parts of eluates, 20%, 50% and 95% ethanol eluate were obtained, separately. Then they were analyzed by high performance liquid chromatography. Chromatographic conditions: chromatographic column was Diamonsil? C18 (4.6 mm × 250 mm, 5 μm), mobile phase was methanol-water in gradient elute, flow rate was 1 mL·min-1,column temperature was 35 ℃, detective wavelength was set at 332 nm, record time was 40 min. Results: 7 common peaks were obtained in methanol extract, while 11 common peaks were displayed after prepared by maceoporous resin. Conclusion: It can show more information in HPLC fingerprint which samples prepared by macroporous resin. This is a useful method in sample preparation.  
        关键词：Flos Genkwa;macroporous resin preparation method;HPLC;fingerprint   
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        发布时间：2024-01-04
      • LI Li,ZHANG Cun,XIAO Yong-qing,Lin Na,LIU Chun-fang,PANG Zhen,LI Gui-liu,CHEN Dong-dong,TIAN Guo-fang
        Vol. 15, Issue 6, Pages: 7-10(2009)
        摘要：Objective:To compare the weight of macroporous resin separation parts and its HPLC profile with the different Chinese herbal pieces from Rheum palamatum L., and to explore the effective substance of the processed herbal medicine. Method: 75% ethanol was used for extraction. After the separation by the macroporous resin, four parts were analyzed by HPLC analysis. Result:The total extraction weight from high to low is no-parched, liquored>vinegar-baked>braising with liquor>charred. The mass of 50% ethanol elution part is highest in liquored, vinegar-baked and charred. The 20% and 50% ethanol elution part have the same weight in no-parched, however, the braising with liquor represent a degressive tendency. HPLC results demonstrate that the 20%, 50% and 95%ethanol elution parts differences are remarkably. Conclusion: With the increased processing conditions, the composition of chemical constituents and content in different pieces has obvious changed. Extractions weight and HPLC results indicate that the changes of chemical constituents may result in different property among different Chinese herbal pieces from Rheum palamatum L.  
        关键词：Rheum palamatum L.;Chinese herbal pieces;macroporous resin;HPLC;components   
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        发布时间：2024-01-04
      • GUI Shuang-ying1,2,PAN Jun2,WANG Jun1,CHEN Wei-jin3,SHI Qi-hai3
        Vol. 15, Issue 6, Pages: 13-14(2009)
        摘要：Objective:To develop an HPLC method for determination of polygalacic acid in Xiao'er Zhili Syrup.Methods: A C18 column (4.6 mm ×250 mm,0.5 μm)was used with the mobile phase of acetoneitrile-0.2% phosphoric acid (40∶60), at the detection wavelength of 210 nm. The flow rate was 1.0 mL·min-1, and the temperature of column was 30 ℃. Results:Polygalacic acid showed a good linear relationship at a range of 10.575 μg·mL-1～211.5 μg·mL-1, r= 0.999 7. The recovery and RSD was 98.58% and 1.52%(n=6), respectively. Conclusion:The method is simple, accurate and specifical. It can be used for determination of polygalacic acid in Xiao'er Zhili Syrup.  
        关键词：Xiao'er zhili Syrup;polygalacic acid;HPLC   
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        发布时间：2024-01-04
      • FAN Bin1,BAI Dong2,LIU Hong3,SUN Jian1
        Vol. 15, Issue 6, Pages: 15-17(2009)
        摘要：Objective:To develop a GC method for determining the content of borneol in Aiqikang capsule. Methods: Using methyl salicylate as inter-standard, the content of borneol was assayed on a HP-5 capillary column with a FID detector,a temperature program and high pure helium as a carrier gas with the flow rate of 1 mL·min-1. Results: The linear range of borneol was 0.244～3.476 mg·mL-1. The average recovery of borneol was 99.5% with RSD of 2.5%. Conclusion: This method is accurate, reliable and reproducible. It can be used for controlling the quality of Aiqikang Capsule.  
        关键词：GC;Aiqikang Capsule;borneol;content determination   
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        发布时间：2024-01-04
      • CUI Ying,LU Zhong-xiang,LV Lin,YANG Shi-you
        Vol. 15, Issue 6, Pages: 18-20(2009)
        摘要：Objective:To establish the methods for TLC identification and content determination of Baoxinning dispersible tablets. Method: Radix Notoginseng,Chinese Angelica and Fructus Aurantii was identified by TLC, while tanshinone ⅡA was determined by RP-HPLC.Result: The spot on the thin layer plate was clear.The negative contrast was no interference.The linear range of tanshinone ⅡA was 0.042 ～0.672 μg(r= 0.999 9 ).The average recovery was 100.50% with RSD=1.56%(n=6). Conclusion: The method is accurate and simple for the quality control of Baoxinning dispersible tablets.  
        关键词：Baoxinning Dispersible Tablets;Tanshinone ⅡA;TLC;RP-HPLC   
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        发布时间：2024-01-04
      • WAN Kai-hua1,FU Hui-zheng2,LUO Yong-ming2,ZHANG Dong-ming3
        Vol. 15, Issue 6, Pages: 21-23(2009)
        摘要：Objective:To develop a RP-HPLC method for determination of Morin-3-O-α-L-lyxopyranoside and Morin-3-O-α-L-arabopyranoside in Psidium guajava L.. Methods: A Diamonsil C18 column (250 mm×4.6 mm,5 μm) was used,methanol-0.1% phosphoric acid solution (35∶65)as the mobile phase, flow rate at 1.0 mL·min-1,and the detection wavelength was 355 nm. Results: The of calibration curve Morin-3-O-α-L-lyxopyranoside was linear between 0.061 32 ～ 0.143 08 μg(r=0.999 9 ),The calibration curves of Morin-3-O-α-L-arabopyranoside was linear between 0.058 44 ～ 0.136 36 μg(r=1),The average recoveries were 98.6% and 100.7%,The relative standard deviations were 1.0% and 1.7%. Conclusion: The method could be used for determination of Morin-3-O-α-L-lyxopyranoside and Morin-3-O-α-L-arabopyranoside from Psidium guajava L..  
        关键词：RP-HPLC;Psidium guajava L.;Morin-3-O-α-L-lyxopyranoside;Morin-3-O-α-L-arabopyranoside;Assay   
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        发布时间：2024-01-04
      • WANG Hong-zhi,FAN Jun-ting,LIU Yong,LI Wan-qing
        Vol. 15, Issue 6, Pages: 24-25(2009)
        摘要：Objective:To determine the contents of paeoniflorin and hesperidin in Antihernolysis Granule. Methods:A Tianhe C18 Column was used with methanol-1% Phosphoric acid (35∶65) as the mobile phase at the flow rate of 1.0ml/min.The detecting wavelength was set at 270 nm. Results The linearity of paeoniflorin was good in the range of 0.198 4 ～ 0.992 μg (r=0.997 9 ), The linearity of hesperidin was good in the range of 0.211 2 ～1.056 μg(r=0.999 3 ).The average recovery of paeoniflorin was 99.08%, RSD=1.47%(n=6).The average recovery of hesperidin was 100.67%, RSD=1.60%(n=6). Conclusion: The method is simple, reliable, accurate, and can be applied as the quality control method of Antihernolysis Granule.  
        关键词：Antihernolysis Granule;HPLC;paeoniflorin;hesperidin   
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        发布时间：2024-01-04
      • WANG Rui1,ZHAN Xiao-ri2,QIAO Yan-jiang3
        Vol. 15, Issue 6, Pages: 29-32(2009)
        摘要：Objective:To develop a platform of incubation of plasma in vitro for analysis of conponents of traditional Chinese medicine. Methods:The aconitic alkaloids were as one of the examples. Aconitine, mesaconitine and hypaconitine were added to rabbit plasma and incubated for various durations. The plasma samples were processed with acetonitrile to denature and deposit the protein and extracted the three alkaloids, and the acetonitrile solution was analyzed by a RP chromatographic system. Results: The concentration of aconitine, mesaconitine and hypaconitine in plasma was descreased with the prolonged incubation time,the hydrolysis rate of mesaconitine was the fastest,aconitine was the second and hypaconitine was the third. Conclusion: A simple, stable and controllable method has been established, which has some reference value for pharmacokinetic study of aconitic alkaloids.  
        关键词：Radix Aconiti lateralis;aconitic alkaloids;in vitro experiment   
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        发布时间：2024-01-04
      • JIANG Min1,2,XIA Xin-hua1
        Vol. 15, Issue 6, Pages: 33-36(2009)
        摘要：Objective:To study the flocculation process with chitosan for Xiang A-2 Granules. Methods On the basis of the one-factor investigation such as the amount of chitosan, the concentration and pH of the extracting solution,temperature and stirring etc,the experiments for the further optimization of flocculation process conditions were arranged with orthogonal design.Results:The optimal flocculation process conditions were as follows. The adding amount of chitosan was 0.6 mL·g-1, the extract solution and medicinal materials were in the ratio of one to four 1∶4,the PH of the solution was 7,the flocculation temperature was 60 ℃, the stirring speed was 200 r·min-1 and the stirring time was 10 minutes. Conclusion:The flocculation process with chitosan can be used for the purification of Xiang A-2 Granules.  
        关键词：Xiang A-2 Granules;chitosan;flocculation;paeoniflorin;HPLC   
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        发布时间：2024-01-04
      • ZHANG Lei1,LIU Jian-gang1,SHI Da-zhuo1,FENG Jia-tao1,LU Xiao-yan2,YANG Xiao-ping2,LIANG Xin-miao2
        Vol. 15, Issue 6, Pages: 37-40(2009)
        摘要：Objective:To observe the effect of active component injection of Slauia miltiorrhiza Bge. and Carthamus tinctorius L. (salvianolic acid B and hydroxysafflor yellow A) on myocardial ischemical reperfusion injury in rats. Methods:Myocardial ischemical reperfusion injury model of SD rat was established by ligation of left anterior descending branch for 40 min,and then reperfusion for 120 min. Ten minutes later after coronary ligation, the active component was injected intravenously. The rats were divided into 6 groups (n=10,each group),they were model group,sham operation group,Danhong injection group,low dose,moderate dose and large dose groups of active component injection of Slauia miltiorrhiza Bge.and Carthamus tinctorius L..The degree of myocardial infarction in rats was determined for every group after 2 h of reperfusion, the contents of cTnT and CK-MB were detected. ST-T change in ECG,TXB2,6-Keto-PGF1α and platelet aggregation in blood plasma were investigated.Results:Compared with model group, every group of active component injection of Slauia miltiorrhiza Bge.and Carthamus tinctorius L. can significantly lower the increase of cTnT and CK-MB in rats(P<0.01), moderate dose and large dose groups can remarkably lower the maximum platelet aggregation and TXB2 in rats (P<0.01, P<0.01),correct the unbalance of 6-Keto-PGF1α /TXB2,lower the ST-T extent of ECG in 30 min of reperfusion. Conclusion:Active component injection of Slauia miltiorrhiza Bge.and Carthamus tinctorius L.can lower platelet aggregation induced by reperfusion injury in rats,reduce the leakage of impaired myocardial creatase,inhibit the production of thromboxan, prevent the formation of thrombus,improve the myocardial microcirculation and ischemia.  
        关键词：myocardial ischemic;ischemical reperfusion injury;salvianolic acid B;hydroxysafflor yellow A;platelet aggregation   
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        发布时间：2024-01-04
      • WANG Xi,ZHANG De-qin,LI Yu-hong,LI Jun-qing,ZHANG Xiao,ZENG Sen
        Vol. 15, Issue 6, Pages: 41-43(2009)
        摘要：Objective:To observe the effect of Tangzhiqing(TZQ) on lipid metabolism in hypercholesterolemia rabbits. Methods:The model of hypercholesterolemia rabbit set up by feeding hypercholesterol diet. Then we used TZQ to treat the Hypercholesterolemia rabbits for 30 days at the dose of 3.2, 1.6, 0.8 g crude drug·kg-1, compared with Xuezhikang(0.13 g·kg-1). At the end,the serum and hepatic total cholesterol(TC),triglyceride(TG) and the serum FFA were measured. Results:Compared with the model group, the levels of TC, TG and FFA obviously decreased by TZQ (P<0.05,P<0.01). Conclusion:TZQ could effectively regulate lipid metabolism.  
        关键词：hypercholesterolemia;Tangzhiqing;lipid metabolism;free fatty acid   
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        发布时间：2024-01-04
      • FU Hong,WANG Xue-mei,LIU Geng-xin
        Vol. 15, Issue 6, Pages: 44-46(2009)
        摘要：Objective:To observe the effect of Modified Wuzi Yanzong Prescription and its components on spatial behavior in the rats with Alzheimer’s disease(AD) by Aβ25-35.Methods:The animal model of AD was established by Aβ25-35 stereotactic injection into the lateral cerebral ventricle of rats. Rats were treated with Modified Wuzi Yanzong Prescription and its components (total flavonoids and total polysaccharodes) for 15 days,Morris water maze with the video record and computer system and the spacial exploration experiment were used to assess the behavior performances of the rats. Results: Significant differences were observed between model group and Modified Wuzi Yanzong Prescription and its components treated groups (P<0.05).Compared with the model group, the escape latency and the searching distance were markedly reduced (P<0.05),the percentage of the rats swimming time and distance in platform quadrant of the spacial experiment were markedly higher(P<0.05,P<0.01)in Modified Wuzi Yanzong Prescription and its components treated groups. Conclusion: Modified Wuzi Yanzong Prescription and its component can improve learning and memory disorders of the model rats induced by Aβ25-35.  
        关键词：Modified Wuzi Yanzong Prescription;flavonoids;polysaccharides;learning and memory;β-amyloid peptide 25-35;maze learning   
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        发布时间：2024-01-04
      • LIN Xu-kai1,XIAO Yong1,HAN Jian2,PAN Huai-geng3,ZHANG Yi-peng1
        Vol. 15, Issue 6, Pages: 47-49(2009)
        摘要：Objective:To observe the protective effect of Weishu Powder (WP) on experimental acute gastritis in rat. Methods: fifty-six SD rats were randomized into normal group, model group, low-dose WP group, high-dose WP group,low-does Chen-xiang-lu-bai-lu tablet (Chen) group, high-dose Chen group and Cimetidine group. The rats in the above groups were fed with the corresponding drugs by gastric infusion for 3 days. The injury of stomach mucous membrane induced by alcohol and acute gastritis induced by sodium salicylate in rats were observed. Effect of WP on the count of somatic wrenching reaction induced by dilute acetic acid in mice were determined. Results: As compared with the model group, two dose groups of WP could depress the injury of stomach mucous membrane induced by alcohol and reduce the ulceration index of acute gastritis induce by sodium salicylate (P<0.05 or P<0.01), also decreased the count of somatic wrenching reaction in mice. The different effects between WP and Chen in the same dose was significant (P<0.05 or P<0.01). Conclusion: WP possess markedly protective effect on the injury of stomach mucous membrane in rat, it also reduced the wrenching count in mice.The effect of WP was superior to Chen in the same dose.  
        关键词：Weishu Powder;acute gastritis;somatic wrenching reaction;rat   
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        发布时间：2024-01-04
      • ZHENG Shu-guo1,QIAN Zhi-yu2,WANG Hong-ting1
        Vol. 15, Issue 6, Pages: 50-52(2009)
        摘要：Objective:To study the effects of crocetin on the susceptibility of low density lipoprotein (LDL) to oxidation and serum level of oxidized LDL (Ox-LDL) in hyperlipidemic rabbits and the possible mechanisms involved. Methods: Hyperlipidemic rabbits were established by feeding the animals with a high lipid diet for 4 weeks. Serum level of Ox-LDL was determined by sandwich ELISA. Following isolation of LDL, the susceptibility of LDL to oxidation was evaluated by in vitro assay. Serum levels of lipids, serum total antioxidant capacity (TAC) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were also determined. Results: Crocetin of dosages of 30 and 15 mg·kg-1 markedly reduced serum level of Ox-LDL and the susceptibility of LDL to oxidation, while serum levels of lipids were reduced only by 30 mg·kg-1 of crocetin. Both dosages of crocetin significantly enhanced serum TAC and the activities of SOD and GPX. Conclusion: Crocetin significantly reduces serum level of Ox-LDL and the susceptibility of LDL to oxidation in hyperlipidemic rabbits, which might be attributed, at least partly, to the enhancement of serum TAC and the activities of SOD and GPX.  
        关键词：crocetin;hyperlipidemia;ox-LDL;antioxidant;atherosclerosis   
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        发布时间：2024-01-04
      • KAN Hong-wei1,4,WEI An-xiang2,YU Dong-an2,HUANG Shi-fu3,YANG Shi-you1,4,XU Peng-fu1,4
        Vol. 15, Issue 6, Pages: 56-58(2009)
        摘要：Objective:To study the effect of Xinshi Injection (XSI) on cerebral blood flow (CBF), cerebrovascular resistance (CVR), concentration of SOD, MDA and NO of brain tissue in anesthetized dogs. Methods: Healthy dogs were divided into normal sodium (NS) control group, Danshen injection group and XSI high dose group (4 g·kg-1), middle dose group(2 g·kg-1) and low dose group (1 g·kg-1). After anesthetized by 3% carbrital, the femoral vein of left lower extremity (LLE) was separated to iv XSI; the left common carotid artery was separated to measure the blood flow of internal carotid artery; the femoral artery of LLE was separated to determine mean arterial blood pressure, then the CBF and CVR were calculated. The concentration of SOD, MDA and NO were measured by kits respectively. Results: XSI increased the blood flow in brain tissue and decreased CVR significantly. It also increased concentration of SOD and decreased concentration of MDA and NO in the brain tissue. Conclusion: XSI can improve blood flow of dog's brain and dilate cerebral vessels of dog.  
        关键词：Xinshi Injection;cerebral blood flow;cerebrovascular resistance;antioxidant   
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        发布时间：2024-01-04
      • FU Hui-ying1,SHOU Qi-yang1,LI Yan-wei2,ZAI Yun-yan2,LV Gui-yuan1
        Vol. 15, Issue 6, Pages: 59-61(2009)
        摘要：Object:To investigate the effect of Hirsutella sinensis strain fermentation on sub-health rats with sleep-deprivation.Methods: Continuous sleep deprivation of 96 hours caused sub-health in rats. After Hirsutella sinensis strain fermentation administration, the rats are detected those index, such as weight,organ index,blood pH, PaO2, SO2%, SOD and MDA. Results:The experimental result indicate that fermentation of Hirsutella sinensis strain can improve physical sign, gain weight, regulate blood pH, enhance PaO2 and SO2%, improve hepatic and renal function, retrieve free radical metabolism in sub-health rats.Conclusion: Hirsutella sinensis strain fermentation is benefit to sub-health rats.  
        关键词：fermentation of Hirsutella sinensis strain;sub-health;sleep deprivation   
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        发布时间：2024-01-04
      • TANG Yu-ping,WU Qi-cheng,DING An-wei,DUAN Jin-ao
        Vol. 15, Issue 6, Pages: 79-81(2009)
        摘要：The eighteen incompatible medicaments and nineteen medicaments of mutual restraint have been tought of traditional prohibited combination in long time,but people are unable to agree upon interrelated theories and can not reach an accordant consideration.From their origins and different comprehension of present TCM,this paper sum up and analyze previous experimental results in toxicology,pharmacology,chemistry and informatics so as to comprehend the scientific significance of the eighteen incompatible medicaments and nineteen medicaments of mutual restraint.We suggest that every drug pair has its own characteristic,one or several pairs can not typif the whole Eighteen Incompatible medicaments and nineteen medicaments of mutual restraint.Therefore the accurate modern understanding can only be reached until a systematic reseach on the whole drug pairs is done.  
        关键词：eighteen incompatible medicaments;nineteen medicaments of mutual restraint;prohibited combination;modern realization   
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        发布时间：2024-01-04
      • GUO Yu-jie,MENG Shuo,XU Hui,LIU Jian-xun
        Vol. 15, Issue 6, Pages: 82-85(2009)
        摘要：The action principles of Chinese traditional medicines feature multi-active components, multi-paths, multi-target sites. One of the key points in Chinese traditional medicine research is the study of herbal pharmacokinetics. The chief active constituent and effective part of Radix puerariae lobatae are puerarin and flavone. The pharmacokinetics researches for the two components are summed up, including biological specimen pretreatment, sample identification, absorption, distribution, metabolism and excretion in vivo.  
        关键词：puerarin;flavone of radix puerariae;pharmacokinetics   
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