最新刊期
卷 23 ， 期 12 ， 2017
- 摘要：Objective: To compare the quality control indexes and chemical constituents of crude and processed products of Achyranthis Bidentatae Radix. Method: According to standard in the 2015 edition of Chinese Pharmacopoeia,the quality control indexes and chemical constituents of crude and processed products of Achyranthis Bidentatae Radix were investigated,such as water content,total ash content and alchohol soluble extract content.UPLC-Q-TOF/MS was established to compare the contents of major constituents in crude and processed products of Achyranthis Bidentatae Radix.Moreover,the linearity,precision,stability,repeatability and recoveries of the approach were well validated. Result: The water content,total ash content and alchohol soluble extract content of crude and processed products of Achyranthis Bidentatae Radix all met the requirements of the 2015 edition of Chinese Pharmacopoeia.Ten major constituents were tentatively deduced.Compared with these of the crude products,the contents of benzyl glucoside,benzyl glucoside isomer,polypodine B,β-ecdysterone and ginsenoside Ro in the wine processed products were significantly increased.The contents of zingibroside R1,bidentatoside Ⅰ and chikusetsusaponin Ⅳ were significantly decreased.For the salt processed products,the contents of benzyl glucoside,benzyl glucoside isomer,achyranthesterone A,polypodine B,β-ecdysterone and inokosterone were significantly increased.The contents of zingibroside R1,ginsenoside Ro,bidentatoside Ⅰ and chikusetsusaponin Ⅳ were significantly decreased. Conclusion: The contents of phenolic glycosides and steroidal saponins are significantly increased,while the content of triterpenoid saponins is significantly decreased,which may be degraded under heating condition.关键词：Achyranthis Bidentatae Radix;wine-processed products;salt-processed products;total ash;triterpenoid saponins;β-ecdysterone;polypodine B
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发布时间：2024-01-04 - 摘要：Objective: To study the hemolytic effects of Lonicerae Japonicae Flos and Lonicerae Flos in vitro and to evaluate their potential hemolysis risks. Method: Hemolysis of the rabbit red blood cells were determined by ultraviolet-visible spectroscopy(UV-VIS) after adding the different concentrations of the compounds of saponins,the active fractions of Lonicerae Japonicae Flos,and the samples of Lonicerae Japonicae Flos and Lonicerae Flos,respectively.Then,the hemolysis rates curves can be obtained by plotting the hemolysis rates against the corresponding concentrations. Result: The hemolytic effects of loniceroside A,loniceroside B,macranthoidin A,dipsacoside B and asperosaponin Ⅵ can't be observed as the concentrations were ≤ 1.0 g·L-1.The hemolysis rate of macranthoidin B was increased with the increasing of the concentration.The hemolysis of macranthoidin B can't be observed as the concentration was less than 0.6 g·L-1.Then,the hemolysis rate was 11.7% as the concentration was 0.8 g·L-1 and the hemolysis rate was up to 50.4% as the concentration was 1.0 g·L-1.The hemolytic effects of the active fractions of Lonicerae Japonicae Flos were in the order of total saponins > total phenolics > total iridoids.The hemolysis of the total iridoids can't be observed as the concentration was between 0.1 g·L-1 and 1.2 g·L-1.There was no hemolysis for the total phenolics as its concentration was less than 1.0 g·L-1 .The hemolysis of the total saponins was observed as the concentration was 0.6 g·L-1.Then,it was increased rapidly with the increase of the concentration and the hemolysis rate was up to 55.3% as the concentration was 1.2 g·L-1.The hemolysis curves of the cultivated and wild samples of Lonicerae Japonicae Flos were similar with that of Lonicerae Flos.Their obvious hemolysis concentration was between 2.0 g·L-1 and 4.0 g·L-1. Conclusion: There are no significant differences between Lonicerae Japonicae Flos and Lonicerae Flos concerning their hemolysis.All saponins compounds studied have no hemolysis or weak hemolytic effects. The total saponins of Lonicerae Japonicae Flos also have weak hemolytic effects with the concentration of 0.6 g·L-1, which is equal to 20 g·L-1 of crude drug. However, it is hard to reach such high concentration in the traditional Chinese medicine injections. Therefore, Lonicerae Japonicae Flos and Lonicerae Flos contained the components of saponins could be applied to traditional Chinese medicine injections and the potential hemolysis risks are small on clinic.关键词：Lonicerae Japonicae Flos;Lonicerae Flos;ultraviolet-visible spectroscopy;hemolysis;total phenolics;total saponins;total iridoids
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发布时间：2024-01-04 - 摘要：Objective: To study on distribution of relative molecular mass of Asini Corii Colla hydrolyzate and discuss its blood enrichment and leukogenic effect. Method: TSK-GEL G2000SWXL column (7.8 mm×300 mm) was employed,mobile phase was consisted of 0.1 mol·L-1 NaCl+0.05% NaN3+0.1 mol·L-1 phosphate buffer solution (pH 6.7) and detection wavelength was set at 280 nm;distributions of relative molecular mass of Asini Corii Colla concentrate,bionic hydrolysate and trypsin hydrolysate were determined.The bloodletting method was adopted to establish blood deficiency mice model,leukopenia model induced by cyclophosphamide in mice were used,blood enrichment and leukogenic effect of Asini Corii Colla hydrolyzate was observed. Result: Relative molecular mass of protein and polypeptide in Asini Corii Colla was greater than 6.64×104 Da,relative molecular mass of bionic hydrolysate and trypsin hydrolysate of Asini Corii Colla mainly concentrated in about 3.7×103 Da.Bionic hydrolysate and trypsin hydrolysate of Asini Corii Colla could significantly improve hemoglobin (Hb) andred cell count (RBC)of blood deficiency model mice,and they could significantly increase white blood cell count (WBC),RBC and Hb levels of leukopenia model mice. Conclusion: After the enzymolysis,relative molecular mass of Asini Corii Colla decreases and its blood enrichment and leukogenic effect is significant,effect of bionic hydrolysate and trypsin hydrolysate is roughly equivalent.关键词：Asini Corii Colla;hydrolysate;relative molecular mass;blood enrichment;white blood cell count;red cell count;hemoglobin
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发布时间：2024-01-04 - 摘要：Objective: To explore the differences of inhibition on gastric mucosal injury induced by ethanol in rats between Coptidis Rhizoma(CR) processed with different ginger juice and analyze its mechanism. Method: Rats were randomly divided into the normal group,model group,raw CR group,CR processed by fresh ginger juice (CRFG) group,CR processed by dried ginger juice(CRDG) group,fresh ginger juice(FG) group and dried ginger juice(DG) group.Gastric mucosal injury was induced by alcohol in rats except the normal group.The gastric mucosal injury index was calculated according to the literature scoring method.Histomorphologic changes in gastric mucosa were observed by light microscope.The levels of interluekin-8(IL-8) and tumor necrosis factor-α(TNF-α) and 6-keto-prostaglandin F1α(6-keto-PGF1α) in plasma were determined by enzyme-linked immunosorbent assay. Result: Compared with the model group,the gastric mucosal injury indexes of FG group,DG group,CR group,CRFG group and CRDG group were decreased significantly.Compared with the CR group,the gastric mucosal injury indexes of CRFG group and CRDG group were decreased significantly,and the injury index of CRFG group was lower than that of CRDG group.The levels of IL-8 and TNF-α in plasma were significantly increased and 6-keto-PGF1α was significantly decreased in the model group by comparing with the blank group.After administration of drugs,the levels of IL-8 and TNF-α were decreased and the contents of 6-keto-PGF1α were increased,this effect became stronger after the CR processed with ginger juice. Conclusion: CR processed by ginger juice can enhance the protection of gastric mucosal injury,its mechanism may be through inhibition of production of IL-8 and TNF-α and promotion of production of 6-keto-PGF1α,and FG is better than DG.关键词：Coptidis Rhizoma;Coptidis Rhizoma processed by ginger juice;Zingiberis Rhizoma Recens;Zingiberis Rhizoma;gastric mucosal injury;interluekin-8;6-Keto-prostaglandin F1α
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发布时间：2024-01-04 - 摘要：Objective: To compare anti-inflammatory and antipyretic effect of Sophorae Flavescentis Radix pieces between integrated production process and traditional processing method. Method: Anti-inflammatory and detumescence effects of water extracts of two Sophorae Flavescentis Radix pieces was observed by xylene-induced ear edema in mice.Antipyretic effect of water extracts of two kinds of Sophorae Flavescentis Radix pieces was compared in febrile rats induced by yeast. Result: Compared with the blank group,water extracts of two kinds of Sophorae Flavescentis Radix pieces could reduce the ear edema of mice,but the anti-inflammatory and detumescence effects of the integrated processed pieces was more significant,the inhibition rates of its each dose group were more than 40%.Antipyretic experiment showed that all the three doses of integrated processed pieces could decrease the body temperature of fever rats for 4 hours,and the antipyretic effect was superior to the traditional processed pieces.The contents of five main active ingredients in integrated processed pieces,such as sophocarpine,matrine,N-oxysophocarpine,sophoridine and oxymatrine,were higher than these in traditional processed pieces,their total amount was about 1.3 times to the traditional processed pieces. Conclusion: There has good similarity in the main effect of anti-inflammatory and antipyretic between integrated processed and traditional processed pieces,the integration processing method will standardize production process from the source,and it can shorten the production cycle under the premise of ensuring the quality of pieces.关键词：habitat processing;pieces;processing integration;Sophorae Flavescentis Radix;sophocarpine;matrine;efficacy
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发布时间：2024-01-04 - 摘要：Objective: To understand the overall situations of medicinal resources at the 4th National Census of Traditional Chinese Medicine (TCM) Resources in Liaoning Province, in order to provide basis for reasonable development and utilization of TCM Resources. Method: The Census was conducted according to the technical scheme of the 4th National Census of TCM Resources. The survey method of representative area-sample area-quadrat set-quadrat was adopted for resources of wild medicinal plants. Six types of representative areas, namely grass, meadow, shrub, coniferous forest, broad-leaved forest and theropencedrymion, were selected in the survey. Result: Medicinal resources in 15 counties of 6 cities were surveyed. Approximately 10 000 medicinal plant samples were collected. This paper reports four newly recorded species, namely Pedicularis resupinata var. albiflora, Bupleurum sibiricum var. jeholense, Malva crispa, and Saussurea acromelaena, which belonged to 4 genuses in 4 families. Conclusion: This research paper reports four new species in Liaoning, in order to provide new materials for protection and rational utilization and development of wild medicinal resources in Liaoning.关键词：newly recorded specie;Pedicularis resupinata var. albiflora;Bupleurum sibiricum var. jeholense;Malva crispa;Saussurea acromelaena
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发布时间：2024-01-04 - 摘要：Objective: To compare the contents of 3-O-[β-D-glucopyranosyl-(1→2)] [β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl echinocystic acid-28-O-β-D-glucopyranosyl ester (ECA for short) and 3-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl oleanolic acid-28-O-β-D-glucopyranosyl ester (OLA for short) in leaves of Aralia elata collected from Fenghuang mountain of Heilongjiang Province in different altitudes and different gathering periods. Method: HPLC-ELSD was used to determine the content. The column was Diamonsil C18 (4.6 mm×250 mm, 5 μm). The column temperature was 30℃. The mobile phase was the mixture liquid of acetonitrile and water for gradient elution. The sample size was 10 μL, and the flow rate was 1.0 mL·min-1. The detector was HPLC-ELSD, the temperature of drift tube was 110℃, and the flow rate of carrier gas was 3.2 L·min-1. Result: The two triterpenoid saponins showed a good linearity (r=0.999 8) within their detection range, and the average recovery rate were 97.71% and 98.06%, respectively. The results showed that the content of OLA was higher than ECA in various batches of samples. From June to September, the contents of the two saponins showed an increasing trend. The contents increased significantly from June to August, but slowly from August to September. In addition, there were no significant difference in content of the two saponins between high altitude and low altitude. Conclusion: The method is simple, rapid and reproducible, and can provide a basis for the quality control of leaves of A. elata from Fenghuang Mountain.关键词：leaves of Aralia elata;triterpenoid saponins;different altitudes;different gathering periods;comparison of contents
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality evaluation method for Arnebiae Radix based on using DNA barcoding technique, and quantification methods for determination of the active components by HPLC, in order to evaluate the quality of Arnebiae Radix. Method: To identify comfreys collected from different resources by DNA barcoding technique, and analyze the naphthoquinones of Arnebiae Radix by chromatography method. Thermo BDS Hypersil C18 column was used with Acetonitrile-pure water-0.2%acetic acid as mobile phase in gradients elution. The flow rate was 0.8 mL·min-1. The UV detector was monitored at 275 nm, and the column temperature was 35℃. Result: The 11 herbs of different resources were identified as Arnebiae Radix by DNA barcoding techniques. The active components from Arnebiae Radix were analyzed by HPLC chromatography. The components were separated completely under chromatography, and the average recoveries were from 95.26% to 101.40%,its RSD values are between 0.7% and 1.8%(n=6). Conclusion: The methods of molecular identification and HPLC chromatography were combined to evaluate the quality of medicinal materials of shikonin, which are simple and easy to operate. It can be used as quality control methods for shikonin.关键词：Arnebiae Radix;DNA barcode;molecular identification;naphthoquinones;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To identify the components in Tanreqing and clarify the differences between Tanreqing capsule and Tanreqing injection in chemical compositions. Method: An HPLC-ESI-MS/MS method was established and separation was performed with acetonitrile and 0.1% formic acid as mobile phase for gradient elution. Mass spectrometric data were obtained in electrospray ionization (ESI) negative ion mode, and the chemical components were identified with quasi-molecular ions and secondary fragment ions. Result: Forty-one compounds including organic acids (7 compunds), flavonoids (10 compounds), iridoids (4 compunds), lignans (3 compounds), phenylethanoid glycosides (9 compounds), bile acids (4 compounds) and 4 unknown components were detected in Tanreqing capsule by HPLC-ESI-MS/MS. Moreover, the chemical structures of 25 compounds were identified, and the chemical structures of 16 compounds (including 4 unknown components) were inferred by their fragmentation patterns. However, only twenty three compounds were found in Tanreqing injection with the losses of 18 chemical compositions including three isolates of suspensaside, sweroside, forsythoside B, isoforsythiaside A, forsythiaside A, rutin, two isolates of suspensaside A, hyperin, aldosecologanin, two isolates of pinoresinol-β-D-glucoside/epipinoresinol-4'-O-glucoside/matairesinol-4'-O-glucoside, phillyrin, baicalein, taurochenodeoxycholic acid (TCDCA) and one unknown compound derived from Scutellariae Radix extract. These differences in compositions between the two preparations may be caused by active carbon adsorption, high temperature sterilization, and blending system in aseptic production process and ultrafiltration technique in process control of Tanreqing injection product. Conclusion: The different chemical constituents in Tanreqing capsule and injection were characterized by the established HPLC-ESI-MS/MS method, which could provide the basis for the quality control of two preparations.关键词：Tanreqing capsule;Tanreqing injection;chemical components;comparative analysis;HPLC-ESI-MS/MS
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发布时间：2024-01-04 - 摘要：Objective: To study the cyclic dipeptides secondary metabolites from endophytic AR-15-1 in Astragali Radix. Method: AR-15-1 was isolated from the roots of Astragali Radix by the method of histology. The fungus was identified by morphological method combined with molecular biology method, and solid culture method was used for large-scale fermentation of endophytic fungi. Cyclic dipeptides were isolated by column chromatography, Sephadex LH-20, and HPLC, and their chemical structures were elucidated by the spectral analysis. Result: Endophytic fungi AR-15-1 isolated from roots of Astragali Radix were identified as Aspergillus sp. Eight cyclic dipeptides were obtained and elucidated as cyclo-(Pro-Gly) (1), cyclo-(Pro-Leu) (2), cyclo-(Pro-Ile) (3), cyclo-(Pro-Vla) (4), cyclo-(Phe-Gly) (5), cyclo-(Phe-Ala) (6), cyclo-(Phe-Tyr) (7) and cyclo-(Phe-Pro) (8). Conclusion: All cyclic dipeptides were isolated from the endophytic fungi of Astragali Radix for the first time, and most of the cyclic dipeptides of Aspergillus sp. showed good biological activity.关键词：endophytic fungus;Aspergillus sp.;Hengshan Astragali Radix;cyclic dipeptides
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发布时间：2024-01-04 - 摘要：Objective: To simultaneously determine the contents of ten flavonoids (genistin, trifolirhizin, maackiain, isoxanthohunol, kushenolI, sophoflavescenol, kurarinone, norkurarinone, 2-O-methyl-kurarinone and isokurarinone) by using HPLC method, compare the flavonoid contents in cultivated and wild Sophorae Flavescentis Radix. Method: The chromatographic separation was performed on Kromasil C18 (4.6 mm×250 mm, 5 μm) with methanol (A)-0.3% formic acid solution (B) as the mobile phase for gradient elution (0-5 min,50%A; 5-25 min,50%-70%A; 25-35 min,70%A; 35-50 min, 70%-90%A; 50-55 min, 90%A). The flow rate was 0.8 mL·min-1 and the detection wavelength was set at 295 nm. Independent t-test (t-test), hierarchical clustering analysis(HCA) and principal components analysis (PCA) were applied to analyze and evaluate the cultivated and the wild Sophorae Flavescentis Radix. Result: The t-test results showed that P>0.05 for all flavonoids, indicating that there were no significant differences in the contents of flavonoids between cultivated and wild Sophorae Flavescentis Radix. HCA and PCA also indicated that there were no differences in the contents of flavonoids between cultivated and wild Sophorae Flavescentis Radix. The results showed that ten flavonoids were well separated and had a good linear relationship, with r>0.999.The average recovery rate was between 95.0%-105.0%, with RSD<5%, consistent with the content determination requirement. Conclusion: The method is accurate and selective, which could provide reference for the quality control of Sophorae Flavescentis Radix.关键词：Sophorae Flavescentis Radix;flavonoids;cultivated;wild
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发布时间：2024-01-04 - 摘要：Objective: To develop a certified reference material (CRM) of pedunculoside according to the Directives for CRM General and Statistical Principle for Certification(GB/T 15000.3-2008) based on the CRM project approved by Standardization Administration of the People's Republic of China (SAC). Method: Pedunculoside samples were obtained by means of macroporous resin enrichment, high speed counter-current chromatography, and recrystalization from the dried bark of Ilex rotunda, and their structures were confirmed by element analysis, UV, IR, HR-MS, NMR, and XRD. In addition, thin layer chromatography was carried out for identification, and high performance liquid chromatography ultraviolet detection and analysis technology was established. The pedunculoside samples divided into 600 bottles with 5 mg per bottle for homogeneity test, stability test, and cooperative certification by 8 laboratories. Result: The homogeneity of pedunculoside samples was excellent in 95% confidence interval, with good stability within 24 months at 0-8℃. The certified purity value of the pedunculoside samples was 99.60% with an expanded uncertainty of 0.10%(k=2.00). They were approved by the National Standardization Management Committee, and consistent with the technical requirements of the national standard samples. Conclusion: National certified reference materials for pedunculoside were successfully studied and developed for the first time, and their purity was higher than that of samples purchased from markets, so this method can be used in the content determination, testing method validation, as well as testing and quality control of pedunculoside products.关键词：pedunculoside;certified reference material;homogeneity;stability;certification;uncertainty
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发布时间：2024-01-04 - 摘要：Objective: To analyze and identify flavonoids in Rosae Laevigatae Fructus Midhx by using UPLC-Q-TOF-MS. Method: ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7 μm) was adopted and eluted with 0.1% formic acid and 0.1% acetonitrile as the mobile phase,with the flow rate at 0.3 mL·min-1,the column temperature was 35℃,and the injection volume was 5 μL. The collected data was monitored under positive and negative electrospray ion modes. Meanwhile, flavonoids were identified with quasi-molecular ions and secondary fragment ions. Result: Ten flavonoids were identified based on their elemental compositions, MS/MS data and published literatures through rapid separation of ethanol extraction solution with UPLC, which were icariin, kaempferitrin, 6-methoxykampferol-3-O-galactoside, apigenin, hesperidin, hyperoside, rutinum, liquiritigenin, potengriffioside A and quercetin. Conclusion: UPLC-Q-TOF-MS is a rapid and effective technology to analyze flavonoids in Rosae Laevigatae Fructus, and provides an accurate and effective method for the identification of components, the basis for quality control and a reference for defining the material basis of its efficacy.关键词：Rosae Laevigatae Fructus;flavonoids;UPLC-Q-TOF-MS;icariin;kaempferitin
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发布时间：2024-01-04 - 摘要：Objective: To improve the quality standards of Olibanum, and achieve efficient and safe application of Olibanum decoction pieces and its preparations. Method: Olibanum was studied from quality standard and irritation aspects. Boswellic acids in Olibanum were analyzed by TLC, and the total polysaccharide content was determined by phenol sulfuric acid method; gas chromatography was used to determine the content of octyl acetate in the volatile oil of Olibanum, and the irritation of Olibanum was studied by using animal experiment. Result: In the thin layer chromatography of Olibanum, the components of boswellic acid showed the same color spots in the corresponding positions of the chromatogram for reference substances, with no interference in the blank control. The average recovery rate of total polysaccharides in Olibanum was 99.6%, with RSD of 1.2% and average mass fraction of 7.0%. The average recovery rate of octyl acetate in Olibanum volatile oil was 100.16%, with RSD of 0.9%, and average content mass fraction of 0.08%. In addition, volatile oil of Olibanum pieces had irritation. Conclusion: The TLC method for identifying boswellic acids and the determination method for total polysaccharides and octyl acetate in Olibanum were simple and accurate. These methods can be used to improve the quality standards of Olibanum decoction pieces, and provide the basis and protection for quality standards of Olibanum extracts and formulations as well as their efficient and safety use.关键词：Olibanum;quality standard;TLC;determination of content;irritation
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发布时间：2024-01-04 - 摘要：Objective: To study on the mechanism of Siwutang in treating primary dysmenorrhea syndrome from the aspect of endogenous metabolites. Method: Dysmenorrhea rat model was constructed by injecting estradiol benzoate and oxytocin.UPLC-Q-TOF-MS was employed to analyze fingerprint of metabolites in plasma of dysmenorrhea rats under electrospray ionization positive and negative ion mode,the acquired metabolites were identified by mass spectrum informations and public database retrieval.Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were adopted to screen the differential metabolites among the normal group,dysmenorrhea model group and Siwutang group;these different metabolites among three groups were analyzed by KEGG metabolic pathway method. Result: Twenty-three endogenous metabolites from dysmenorrhea model rats were detected and identified;when the dysmenorrhea model group compared with the normal group,progesterone,cortexolone,tauroursodeoxycholic acid and lysophosphatidic acid were the different metabolites with variable importance in the projection (VIP)>1 and P<0.05.When the Siwutang group compared with the model group,L-phenylalanine,L-tyrosine,N-benzoylglycine,L-methionine,cortexolone and stearic acid glycinamide were the different metabolites with VIP>1 and P<0.05. Conclusion: Siwutang has a certain callback effect on the differential metabolites in primary dysmenorrhea rats,its therapeutic mechanism is mainly related to steroid synthesis,lysophosphatidic acid metabolism and amino acid metabolism.关键词：Siwutang;metabolomics;primary dysmenorrhea;principal component analysis;cortexolone;lysophosphatidic acid;L-tyrosine
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发布时间：2024-01-04 - 摘要：Objective: To screen effective substances from Polygonum orientale flower extract by UHPLC-Q-TOF-MS technology for H9c2 myocardial cells. Method: The active substances in P. orientale flower extract were combined with H9c2 myocardial cells under imitated physiological environments,then the unattached substances were washed off by phosphate buffer solution (PBS) with pH of 7.4.After that,the conjugated compounds were eluted by PBS with pH of 4.0.These compounds released from target sites were collected and handled through Oasis HLB solid phase extraction column to be condensed.The separation was performed on a Eclipse Plus C18 column,mass sepectrometric data were obtained in electrospray ionization (ESI) positive and negative modes.Meanwhile,the chemical components were identified by comparing the retention times with standard substance and secondary fragment ions. Result: Seventeen compounds from P. orientale flower extract were identified preliminarily, and eight compounds were identified as protocatechuic acid,cianidanol,kaempferol,kaempferol-3-O-β-D-glucoside,quercetin,quercetin-3-O-β-D-glucoside,kaempferol-3-O-α-L-rhamnoside,paprazine. Conclusion: Throngh analysis of the fracture fluid compounds from the H9c2 cells extraction combined with active cells,the bioactive constituents from P. orientale flower extract interacted with myocardial cells are screened,this research provides a scientific fundament for material basis of effectiveness of P. orientale flower.关键词：UHPLC-Q-TOF-MS;Polygonum orientale flower extract;effective substances;kaempferol-3-O-β-D-glucoside;kaempferol-3-O-α-L-rhamnoside;pharmacodynamic basis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the intervention effect of Bushen Huatan Yizhi decoction(BSHT) in APPV717I transgenic mice and explore its possible mechanism. Method: The 120 three months old APPV717I transgenic mice were randomly divided into two batches, n=60 in each batch. The mice in each batch were randomly divided into model group (APP), Donepezil group (0.000 92 g·kg-1·d-1), BSHT low dose group(5.2 g·kg-1·d-1), middle dose group(10.4 g·kg-1·d-1), and high dose group(20.8 g·kg-1·d-1), n=12 in each group (6 males and 6 females). Another 24 C57BL/6J mice of the same age were set as a normal group, and the mice in model group and normal group received intragastric administration of equivalent volume of distilled water, 1 time/day. The first batch of mice received drug administration for 8 months, and the second batch for 4 months. Behavioral ability was measured in Morris water maze finally, and the expression levels of Tau protein hosphorylation (p-Ser 199/202, Tau-1) in the hippocampus was determined by using enzyme linked immunosorbent assay(ELISA) and Western blot. Result: Behavioral experiment results showed that, as compared with the normal group, the latency was prolonged in model group mice(P<0.05, P<0.01). After months treatment, the latency was shortened in various treatment groups as compared with model group(P<0.05). ELISA and Western blot assay showed that as compared with the normal group, the phosphorylation degree of Ser199/202 phosphorylation sites in hippocampal neurons was increased significantly in model group(P<0.01); and the phosphorylation degree of Ser199/202 phosphorylation sites in various treatment groups was significantly lower than that in model group(P<0.05, P<0.01). Conclusion: Bushen Huatan Yizhi decoction can improve the phosphorylation degree of Ser199/202 phosphorylation sites, prevent neurofibrillary tangle(NFT) formation, and thus improve the early learning and memory abilities in APPV717I transgenic mice.关键词：Bushen Huatan Yizhi decoction;Alzheimer's disease;APPV717I transgenic mice;Morris water maze;learning and memory ability;neurofibrillary tangle;Tau protein hosphorylation
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发布时间：2024-01-04 - 摘要：Objective: To study the regulation effect of Anshen Dingzhiling (ADL) on tyrosine hydroxylase (TH), DOPA decarboxylase (DDC), and protein kinase A (PKA), and reveal the effect mechanism of Anshen Dingzhiling in treatment of attention deficit hyperactivity disorder (ADHD). Method: Spontaneously hypertensive (SHR) rats were divided into model group, western medicine group, ADL high, medium and low dose (26.70,13.35,6.75 g·kg-1) groups based on random number, n=8 in each group. Another 8 WKY rats were set as normal group. The rats in each group received lavage administration for 4 weeks. Brain synaptosome was prepared by using Percoll density gradient centrifugation method. Western blot method was used to detect the protein expression levels of TH and PKA;Real-time polymerase chain reaction (Real-tim PCR) technique was used to detect mRNA expression levels of TH, DDC, PKA; and immunofluorescence technique was used to detect TH, and DDC positive cells expression. Result: As compared with normal group, the synaptic TH, DDC, and PKA expression levels were significantly reduced in model group (P<0.01);as compared with model group, the synaptic TH, PKA protein expression levels, TH, PKA, DDC mRNA expression levels, and number of positive cells were increased in western medicine group and ADL medium dose group (P<0.05). Immunohistochemistry positive cell count results showed that as compared with normal group, TH positive cells expressing level was significantly reduced in model group and ADL high dose group (P<0.01), and TH positive cells expressing level was reduced in ADL low dose group (P<0.05); DDC positive cells expressing level was significantly reduced in model group and ADL low dose group (P<0.01). As compared with model group, TH positive cells expressing level was significantly increased in western medicine group and ADL low dose and medium dose groups (P<0.01); in addition, number of DDC positive cells was increased significantly in the western medicine group and ADL medium and high dose groups (P<0.01). Conclusion: ADL could affect the speed of dopamine synthesis through regulating the expression of TH, DDC, PKA, and the effect of ADL for core symptoms of ADHD may be related to the regulation of dopamine synthesis speed.关键词：Anshen Dingzhiling;tyrosine hydroxylase (TH);DOPA decarboxylase (DDC);protein kinase A (PKA);spontaneously hypertensive (SHR) rats;synaptosome
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of extracts from Callicarpa nudiflora(ECN) leaves on platelet activation and to elucidate its mechanisms. Method: The 50 male SD rats were randomly divided into blank group, Yunnan Baiyao group (0.930 g·kg-1), ECN low, medium and high dose groups (0.131, 0.263, 0.525 g·kg-1). All the rats were treated for 10 days. After treatment, the platelets of the rats in all groups were extracted, and microscale plate method was used to determine adenosine diphosphate (ADP)-induced platelet aggregation rate; enzyme linked immunosorbent assay (ELISA) was used to determine thromboxane B2(TXB2) and 5-serotonin (5-HT) levels in platelet as well as cyclic adenosine 5'-monophosphate (cAMP) release level in platelet. Furthermore, the effect of ECN on phosphatidylinositol-3-kinases (p-PI3K) protein expression levels was determined by using Western blot assay. Result: ECN enhanced ADP-induced platelet aggregation significantly (P<0.05), and it could significantly promote TXB2 and 5-HT release (P<0.05). Furthermore, cAMP level in platelet was (3.074±0.538), (3.340±0.265) nmol·L-1respectively in ECN low dose group and high dose group, lower than (3.795±0.586) nmol·L-1 in blank group (P<0.05), and cAMP level in ECN medium dose group was (3.003±0.242) nmol·L-1, significantly lower than that in blank group (P<0.01). In Western blot assay, it was found that ECN could significantly increase p-PI3K kinase protein expression (P<0.01). Conclusion: Collectively, the data presented here demonstrated that ECN enhanced the development of platelet aggregation and amplification of platelet activation. These effects may be associated with its up-regulation on P2Y12 receptors.关键词：Callicarpa nudiflora;platelet;adenosine diphosphate (ADP);P2Y12 receptor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Buzhong Yiqi Wan medicated serum on NOD like receptor-3 (NLRP3) inflammasome and related cytokines in intestinal epithelial cell(IEC-6) injury model. Method: According to the methods of serum pharmacology of Chinese medicine, IEC-6 cells were divided into fetal bovine serum (FBS) group, tumor necrosis factor-α (TNF-α) group, blank group, model group, Buzhong Yiqi Wan group (BZYQ), and sulfasalazine group (SASP). First 10% 2,4,6-nitrobenzene sulfonic acid (TNBS) model serum or TNF-α(mass concentration of 100 g·L-1) was used to stimulate IEC-6 cells for 24 h, and then 10% drug containing serum was added for further culture for 24 h. Then cell proliferation was detected by cell counting kit-8(CCK-8) method and the protein expression level of NLRP3 inflammasome was detected by Western blot; the interleukin-1β (IL-1β) and IL-18 levels in cell culture supernatant were tested by enzyme-linked immunosorbent assay(ELISA). Result: As compared with the blank group, IEC-6 cell proliferation was significantly decreased in both TNF-α group and model group (P<0.01); the protein expression levels of NLRP3 inflammasome and cytokine (IL-1β, IL-18) levels were all increased (P<0.01). After administration with BZYQ medicated serum, cell proliferation rate was increased significantly, and the NLRP3 inflammasome and IL-1β, IL-18 levels were decreased in IEC-6 cells (P<0.05, P<0.01) as compared with model group. Conclusion: BZYQ medicated serum has protective effect on intestinal epithelial cell injury by down-regulating the NLRP3 inflammasome protein expression and cytokine level in injury epithelial cell.关键词：medicated serum;NLRP3 inflammasome;intestinal epithelial cell;Buzhong Yiqi Wan;serum pharmacology
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protection effect of total flavonoids of Puerariae Lobatae Flos on myocardial ischemia-reperfusion injury in rats and explore its possible mechanism. Method: The 72 Wistar rats were randomly divided into sham operation group, myocardial ischemia-reperfusion model group, positive drug control group (Danshen tablets, 240 mg·kg-1), total flavonoids of Puerariae Lobatae Flos low, medium and high dose groups (20, 40, 60 mg·kg-1). The drugs were given for 28 days as preventive administration. The rat models were established by clamping the artery for 30 min. After successful modeling, the areas of myocardial infarction were determined by triphenyltetrazolium chloride(TTC) staining; the morphological changes of myocardial tissues were observed by htoxylin eosin(HE) staining; the levels of total antioxidant capacity(T-AOC), adenosine triphosphate(ATP), adenosine diphosphate(ADP), aspartate aminotransferase(AST), lactate dehydrogenase(LDH), and creatine phosphokinase(CPK) were detected; the levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and IL-1β in myocardial tissues were determined by enzyme-linked immunosorbent assay(ELISA); the levels of Bax, Bcl-2 and Caspase-3 were detected by ELISA. Result: As compared with model group, myocardial infarct size in the total flavonoids of Puerariae Lobatae Flos high dose and middle dose groups was significantly decreased(P<0.05, P<0.01); the histopathological changes and cardiomyocytes apoptosis were improved in total flavonoids of Puerariae Lobatae Flos high dose and middle dose groups, and the effect was most obvious in high dose group; the levels of AST, CPK LDH ADP and ATP in serum, as well as TNF-α, IL-6, IL-1β levels in myocardial tissues were decreased significantly in total flavonoids of Puerariae Lobatae Flos high and middle dose groups(P<0.05,P<0.01); total flavonoids of Puerariae Lobatae Flos high and middle dose groups up-regulated the Bcl-2 content in myocardial tissues of rats, and reduced the Bax and Caspase-3 content. Conclusion: Total flavonoids of Puerariae Lobatae Flos could effectively lower the areas of myocardial infarction, and improve the histopathological changes and cardiomyocytes apoptosis, suggesting that total flavonoids of Puerariae Lobatae Flos had protective effects on rats with myocardial ischemia-reperfusion injury, which may be related to its effects on reducing the infiltration of inflammatory cytokines and decreasing the apoptosis of myocardial cells.关键词：total flavonoids of Puerariae Lobatae Flos;myocardial ischemia-reperfusion injury;myocardial infarction;tissue cell;inflammatory cell infiltration
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of total saponins of Thladiantha dubia root(TSTR) on changes of interleukin-15(IL-15) and interleukin-34(IL-34) in the blood and knee synovial membrane in the treatment of type II collagen-induced arthritis (CIA) rats, and explore the mechanism of TSTR in treating CIA rats. Method: The 80 male Wistar rats were selected, and ten of them were randomly taken as normal control group. The remaining rats were injected with the mixed emulsion of bovine type II collagen and complete Freund's adjuvant in multiple points on back and tail root. After 7 days, it was carried out with the same operation.Fourteen days after the first injection, those with arthritis index (AI value)≧4 points were identified as successful modeling. The successfully modeled rats were randomly divided into model group, TSTR low, medium and high dose groups(20, 40, 80 mg·kg-1) and tripterygium glycoside(TG, 12 mg·kg-1) positive drug group. Rats in each group received corresponding drugs for 35 days by intragastric administration, 1 time/day. Then the paw swelling in rats was observed and AI values were evaluated; the morphological changes of synovium were observed with htoxylin eosin(HE) staining; the levels of IL-15 and IL-34 in serum were detected by enzyme-linked immunosorbent assay(ELISA); Western blot and reverse transcriptase PCR(RT-PCR) were used respectively to measure the protein and mRNA expression levels of IL-15, IL-34 in synovium. Result: As compared with the normal group, AI value was increased in the model group (P<0.01); the number of inflammatory cells in synovial cells was increased, and the levels of IL-15 and IL-34 in serum and synovial tissues were significantly increased (P<0.01). As compared with the model group, AI value was significantly decreased in each dose TSTR groups and TG positive group(P<0.01), and the inflammatory cells number and vascular quantity in the synovial membrane were significantly decreased in TSTR low dose group and the positive drug group (P<0.05, P<0.01); the expression levels of IL-15 and IL-34 in the serum and the expression levels of IL-15 and IL-34 in the synovial membrane were significantly decreased in the low-dose TSTR group and the TG positive group (P<0.05, P<0.01). Conclusion: The therapeutic effect of TSTR on CIA rats may be related to the inhibition of IL-15 and IL-34 expression in blood and synovium.关键词：total saponins of Thladiatha dubia root;collagen II;arthritis;interleukin-15(IL-15);IL-34
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect and mechanism of paeonol (Pae) combined with Notoginseng total saponins(NTS) on tumor necrosis factor tumor necrosis factor-α(TNF-α) and interleukin interleukin-1β(IL-1β) mRNA expression levels in myocardium tissues in rats with ventricular remodeling after acute myocardial infarction (AMI). Method: Totally 80 male SD rats were selected, and 70 of them were randomly used to establish AMI rat models by ligaturing left anterior descending (LAD) artery. Alive and successfully modeled rats were randomly divided into model group, Pae group (8 mg·kg-1), NTS group(40 mg·kg-1), Pae+NTS group low-dose group (Pae 4 mg·kg-1+NTS 20 mg·kg-1), high-dose group (Pae 8 mg·kg-1+NTS 40 mg·kg-1) and captopril positive control group (10 mg·kg-1) according to different interventions, and another 10 rats were used as sham operation group. The rats in various treatment groups received intraperitoneal injection of drugs for 28 days. Then the pathology changes of myocardial tissues were observed by htoxylin eosin(HE) staining; the protein expression levels of NF-κBp65 and p-IκB-α were detected by Western blot assay, and the mRNA expression levels of TNF-α and IL-1β were detected by reverse transcriptase PCR(RT-PCR). Result: As compared with sham operation group, there were obvious myocardial pathological changes in model group, with significantly increased NF-κBp65 and p-IκB-α protein expression levels as well as TNF-α and IL-1β mRNA expression levels (P<0.01). As compared with the model group, the myocardial pathological changes were improved to varying degrees in various treatment groups, with significantly decreased expressions of interest protein and interest mRNA (P<0.01). In addition, the improvement in combined drug use groups was superior to that in single substance drug group (P<0.05, P<0.01), and the effect in high dose combined drug use group was more significant than that in low dose combined drug use group (P<0.05, P<0.01). Conclusion: The combination of Pae and NTS can play obvious synergistic action in improving ventricular remodeling in rats after myocardial infarction, and its mechanism may be associated with blocking the NF-κB/IκB pathway, down-regulating mRNA expression levels of TNF-α and IL-1β, and attenuating the inflammatory reactions.关键词：paeonol;Notoginseng total saponins;durg combination;myocardial infarction;ventricular remodeling
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Buyin Yizhi decoction on learning and memory disorder induced by scopolamine, and investigate its possible mechanism through neurotransmitter and synaptic plasticity related proteins. Method: The learning and memory disorder models were established by intraperitoneal injection of scopolamine (3.0 mg·kg-1) in 50 male Kunming mice. Then these mice were randomly divided into normal blank group, model group and Buyin Yizhi decoction high dose group (18 g·kg-1), middle dose group (6 g·kg-1), and low dose group (2 g·kg-1). The treatment lasted for 2 weeks after modeling. Step-down test, Morris water maze test and novel object test were used to explore the the ability of learning and memory in mice. Meanwhile, the amino acid neurotransmitter levels in prefrontal cortex and hippocampus of the mice were determined by high performance liquid chromatography (HPLC). The expression levels of Synembry(Syn), PSD-95 and brain derived neurotrophic factor(BDNF) in hippocampus were determined by Western blot. Result: As compared with the normal blank group, the latency in step-down test and Morris water maze test was shortened significantly in model group (P<0.01); the electric shocks number was increased (P<0.01); and the exploration time of new object was shortened (P<0.01); at the same time, the contents of gamma-aminobutyric acid (GABA) was increased while the contents of glutamic acid (Glu) were reduced (P<0.01) in hippocampus and prefrontal cortex; and the expression levels of Syn, PSD-95 and BDNF protein were reduced in hippocampus. As compared to the model group, the latency was significantly prolonged in middle and high dose groups (P<0.05, P<0.01); the number of electric shocks in the high dose group was decreased significantly (P<0.05), and the exploration time of new objects was significantly prolonged (P<0.05); GABA content was significantly reduced (P<0.05, P<0.01), while Glu content and the expression levels of Syn, PSD-95 and BDNF protein were significantly increased (P<0.05, P<0.01). Conclusion: These findings suggest that Buyin Yizhi decoction could improve the learning and memory disorder induced by scopolamine and the underlying mechanism may be associated with the increased Glu/GABA ratio in hippocampus and prefrontal cortex and the higher expression levels of Syn and PSD-95 in hippocampus.关键词：Buyin Yizhi decoction;scopolamine;learning and memory;behavior;neurotransmitter;synaptic plasticity
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发布时间：2024-01-04 - 摘要：Objective: To observe the cardioprotective effect of Shenshao capsule and its effective components against myocardial ischemia-reperfusion injury (MIRI) in rats and explore its action mechanism based on the nuclear factor-κB (NF-κB) pathway. Method: Male Wistar rats were used to establish MIRI models by ligation of left anterior descending coronary artery for 30 min and reperfusion for 120 min. Then these rats were randomly divided into sham operation group (Sham), ischemia/reperfusion group (I/R), Shenshao capsule 250 mg·kg-1 group (SS250), Shenshao capsule 500 mg·kg-1 group (SS500), total ginsenoside of Ginseng stems and leaves group (TGSL), paeony group (Pae) and atorvastatin group (Ator). All of these rats received intragastric administration for 7 days. Then the myocardial infarction area was detected by triphenyltetrazolium chloride (TTC) method; the levels of serum creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were measured by automatic biochemical analyzer. The content of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) and IL-10 were determined with enzyme linked immunosorbent assay (ELISA); while Western-blot was used to detect the expression levels of NF-κBp65 and p-NF-κBp65. Result: As compared with I/R group, the myocardial infarction size was reduced in SS250, SS500, TGSL, Pae and Ator groups (P<0.05, P<0.01); CKMB and LDH levels were significantly decreased in SS250, SS500, TGSL and Ator groups (P<0.05, P<0.01). The TNF-α level was decreased in SS250, SS500 and TGSL groups (P<0.05, P<0.01); the IL-1β level was decreased in SS250, SS500, Pae and Ator groups (P<0.05); the level of IL-10 was increased in SS250 and SS500 groups (P<0.05,P<0.01); and the p-NF-κBp65 protein expression level was decreased significantly in SS250 and SS500 groups (P<0.01). Conclusion: Shenshao capsule had cardioprotective effect for MIRI rats, and the mechanism may be associated with inhibiting the over activation of NF-κB expression pathway, and the effect of Shenshao capsule high dose group was superior to its single active ingredient.关键词：Shenshao capsule;myocardial ischemia-reperfusion injury;nuclear factor-κB(NF-κB);inflammation
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Rubiae Radix et Rhizoma 70% ethanol extract on hepatotoxicity, nephrotoxicity and colonic toxicity in rats after long term use, and provide a certain basis for clinical rational drug use. Method: The 48 Wistar rats were randomly divided into blank control group, Rubiae Radix et Rhizoma high dose, medium dose and low dose groups (30, 10, 5 g·kg-1, respectively equivalent to 36, 12, 6 times of clinical human dose) under SPF experimental conditions, n=12 in each group, 6 males and 6 females. The rats in control group received the same volume of distilled water, and all these rat were continuously lavaged for 60 days. During the treatment, the general situations of rats (activities, mental state, diet, color luster, urine color) were observed and recorded. After the last oral administration, the blood biochemical indicators of animals, viscera coefficient and the change of pathological histology were observed. Result: As compared with the blank group, liver, kidney and spleen indexes were increased in each dose Rubiae Radix et Rhizoma groups (P<0.05); the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increased significantly(P<0.01); the levels of β2-MG and Cys-C in male rats were also increased (P<0.05, P<0.01), but in female rats, β2-MG was significantly increased only in high dose group (P<0.01), and there was no difference in Cys-C as compared with blank group. In addition, there was no statistical difference in urea nitrogen (BUN) between each dose Rubiae Radix et Rhizoma groups and blank group, and there were no obvious pathological changes in liver and kidney morphology. Finally, colon tissue morphology, melanin staining and cell apoptosis in each dose group of Rubiae Radix et Rhizoma had no significant differences as compared with the blank control group. Conclusion: Rubiae Radix et Rhizoma 70% ethanol extract had mild hepatotoxicity and nephrotoxicity, but had no toxicity on colon and would not induce melanosis coli (MC) after long term use at the dose ≥5 g·kg-1.关键词：Rubiae Radix et Rhizoma ethanol extract;hepatotoxicity;phrotoxicity;melanosis coli (MC);long-term toxicity
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发布时间：2024-01-04 - 摘要：Objective: To discuss the distribution of Chinese medical syndrome types of post menopause osteoporosis (PMOP) in different age, in order to provide reliable and objective basis for clinical diagnosis and treatment. Method: A total of 751 postmenopausal osteoporosis patients were selected into the study. Four-diagnostic information of the 751 patients, such as Chinese medicine symptoms, signs, tongue pictures and pulse conditions, was collected. χ2 squared inspection analysis was made for the age distribution of traditional Chinese medicine (TCM) syndrome types, and K-means cluster analysis was made on patient information. Result: The age distribution was statistically different from distributions of PMOP basic TCM syndrome type of Yin deficiency and kidney deficiency syndrome (P<0.05). In terms of combination of TCM syndrome types, groups in different age had their own characteristics, the cluster analysis results showed that during the period of perimenopausal syndrome complex between 45 and 54 years old, with the growth of the age, the changes in syndrome types tended to be stable, and kidney and liver deficiencies were particularly prominent. Conclusion: With the growth of the age, the patients' personal circumstances are correlated with the bone mineral density. Most of PMOP patients had kidney deficiency and liver deficiency syndromes. In the aspect of combination of TCM syndrome types, TCM syndrome types in different age have their own characteristics.关键词：postmenopause osteoporosis;bone mineral density;traditional Chinese medicine syndrome;K-means cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Buyang Huanwu Tang on cerebral vascular reserve function in patients with Qi deficiency and blood stasis type cerebral infarction by using transcranial Doppler (TCD) combined with breath holding test, and to observe the clinical efficacy and neurological deficit scores in two groups. Method: The 150 patients with Qi deficiency and blood stasis type cerebral infarction were randomly divided into 2 groups:the treatment group of 78 cases and the control group of 72 cases. Patients in both groups received standardized western medicine treatment:aspirin enteric-coated tablets, 100 mg/times, 1 time/day; or Clopidogrel Bisulfate tablets, 75 mg/times, 1 time/day; medicines for reducing intracranial pressure, brain protective drugs, statin lipid-lowering therapy or rehabilitation therapy was given to regulate blood pressure and blood sugar according to actual conditions. Based on the western medicine treatment, patients in control group added Sanqi Tongshu capsules, 0.2 g/times, 3 times/day, but the patients in treatment group added Buyang Huanwu Tang on the basis of western medicine treatment, 180 mL/time, 2 times/day. Before and after treatment, TCD combined with breath holding test was used to determine the cerebral vascular reserve (CVR), breath holding index (BMI) and mean velocity (Vm) rate; in addition, the clinical efficacy and NIHSS scores were observed in both groups. Result: The total effective rate was 91% in treatment group, better than 76.4% in control group (P<0.05). Day 7 and day 14 after treatment, the NIHSS scores in treatment group were significantly reduced (P<0.05), and they were lower than those in control group (P<0.05). On day 14 after treatment, the levels of CVR, BMI and Vm were increased in treatment group (P<0.05), and the level of CVR in treatment group was higher than that in conrol group (P<0.05). Conclusion: On the basis of routine treatment of Western medicine, Buyang Huanwu Tang can improve the cerebral vascular reserve function in patients with Qi deficiency and blood stasis type cerebral infarction, so as to improve neurological function and improve clinical efficacy.关键词：cerebral infarction;Qi deficiency and blood stasis;Buyang Huanwu Tang;cerebral vascular reserve;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of modified Bushen Huoxuetang combined with acupuncture at Baihui and Sishencong points in treatment of vascular dementia of liver and kidney deficiency type. Method: A total of 180 cases of vascular dementia of liver and kidney deficiency type were randomly divided into the modified Bushen Huoxuetang group, the acupuncture group and the acupuncture combination with modified Bushen Huoxuetang group, with 60 cases in each one. Modified Bushen Huoxuetang was provided in the modified Bushen Huoxuetang group; the acupuncture at Baihui and Sishencong points group was applied the acupuncture group; the acupuncture combined with modified Bushen Huoxuetang was adopted in the acupuncture combination with traditional Chinese medicine (TCM) group. The course of treatment was 28 days. Hastgawa Dementia scale (HDS), activities of daily living (ADL) and mini-mental state examination (MMSE) were applied to compare the efficacy of the 3 groups before and after treatment. Superoxide dismutase (SOD), calcitonin gene-related peptide (CGRP) and serum levels of malondialdehyde (MDA) were detected. Result: The efficiency of modified Bushen Huoxuetang combination with acupuncture group was 91.2%, which was better than 79.7% of the acupuncture group and 70.7% of the modified Bushen Huoxuetang group (P<0.01). After treatment, the score of HDS, ADL and MMSE were improved significantly (P<0.05) in three groups. The acupuncture combination with TCM group was superior to the other groups; SOD, CGRP and MDA in the modified Bushen Huoxuetang combination with acupuncture group were better than those of the other two groups (P<0.05). Conclusion: Modified Bushen Huoxuetang combination with acupuncture at Baihui and Sishencong points could remarkably increase HDS, ADL and MMSE sores of vascular dementia of liver and kidney deficiency type, improve SOD, CGRP and MDA, with a superior clinical efficacy to single administration of modified Bushen Huoxuetang or acupuncture at Baihui and Sishencong points.关键词：acupuncture at baihui and sishencong points;modified Bushen Huoxuetang;liver and kidney deficiency;vascular dementia;clinical observation
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Jiawei Shengjiangsan on pleural effusion adenosine deaminase (ADA), peripheral regulatory blood T cells (Treg) and dendritic cells (DC) cell subsets of patients with tuberculous pleurisy (TBP) with fluid-retention in chest and hypochondrium. Method: A total of 134 cases of TBP patients treated at our hospital from January 2014 to September 2015 TBP were selected and divided into two groups according to the random number table method. Control group was given the routine therapy of pleural effusion and anti-tuberculosis drugs. In addition to that, observation group was given Jiawei Shengjiangsan. Clinical efficacy, pleural effusion ADA levels and percentage ratio of peripheral blood Treg and DC cell subsets accounted for peripheral blood mononuclear cells (PBMC) of two groups after continuous treatment for 2 months were compared. Result: After continuous treatment for 2 months, the total effective rate of observation group was 95.52%, which was obviously higher than 83.58% in control group (P<0.05). The ADA levels of pleural effusion after treatment for 2 months in two groups were significantly lower than those before the treatment (P<0.01). Compared with that of control group, the ADA level of pleural effusion was significantly lower in observation group after treatment for 2 months (P<0.01). Compared with before the treatment, CD25+/PBMC, CD25+/CD4+ and CD25+FoxP3+/CD25+ of two groups after treatment for 2 months decreased significantly (P<0.01). And after treatment for 2 months, the improvement of peripheral blood Treg cell subsets in observation group was obviously lower than that of control group of the same time period (P<0.01). After treatment for 2 months, DC1/PBMC, DC2/PBMC and DC1/DC2 of two groups were significantly higher than before the treatment (P<0.05). And compared with that of control group, the improvement of the peripheral blood DC cell subsets in observation group after treatment for 2 months was more obvious (P<0.05). Conclusion: The application of Jiawei Shengjiangsan for TBP patients in addition to routine western medicine therapy can effectively improve the clinical symptoms, reduce the level of ADA in pleural effusion, and adjust cellular immunity, achieve an accurate effect, and provide new ideas for the prevention and treatment of TBP.关键词：Jiawei Shengjiangsan;fluid-retention in chest and hypochondrium;tuberculous pleurisy;pleural effusion ADA;peripheral blood Treg cell subsets;peripheral blood DC cell subsets
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Yaoshi Xinbao decoction combined with western medicine in the treatment of coronary heart disease and observe its effect on serum homocysteine (HCY) level and blood coagulation function. Method: The 200 patients with blood stasis syndrome coronary heart disease from January 2015 to January 2016 in Department of cardiology of our hospital were randomly divided into control group (102 cases) and observation group (98 cases). Patients in control group were treated with conventional western medicine, and patients in observation group received additional Yaoshi Xinbao decoction based on the treatment in control group for 8 weeks. The two groups of patients were compared for serum homocysteine (HCY) and coagulation function (prothrombin time, activated partial thromboplastin time, fibrinogen). Result: The improvement degree of coagulation function and serum HCY level in observation group was better than that in control group after treatment (P<0.05); observation group could significantly reduce the serum HCY level in patients with CHD (P<0.05), and regulate the blood coagulation function (P<0.05), with significant differences between the groups, especially in the improvement of coagulation function (P<0.05). Control group had no significant differences before and after treatment. Conclusion: Yaoshi Xinbao decoction combined with western medicine has clear clinical curative effect in treatment of coronary heart disease, which can effectively reduce the level of HCY in CHD patients and regulate blood coagulation function, so it is worthy of clinical promotion.关键词：medicinal and edible plant;coronary heart disease;cysteine;coagulation function;blood stasis syndrome
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Guanxin granules on stable angina pectoris with heart-blood stasis syndrome and investigate its effect on platelet activation. Method: One hundred and fifty-four patients were randomly divided into control group (77 cases) and observation group (77 cases) by random number table. In observation group, the patients took aspirin enteric-coated tablets 0.1 g, 1 time/day, trimetazidine dihydrochloride tablets, 20 mg, 3 times/day. In addition, the patients with high blood fat, high blood pressure, or diabetes got symptomatic treatment (secondary prevention of coronary heart disease). Based on the treatment of control group, patients in observation group got Guanxin granules, 1 dose/day in both morning and evening. The treatment course was 20 weeks in both groups. Attack times of angina pectoris, duration of attack, degree of pain, inducing factors and the relationship with physical activity were recorded. Before and after treatment, changes of electrocardiogram and blood lipid were measured. Scores of seattle angina scale (SAQ) and heart-blood stasis syndrome were graded; levels of high sensitive C reactive protein (hs-CRP), homocysteine (HCY), serum cystatin-C (Cys-C), platelet membrane glycoprotein CD63 and CD42 b, and matrix metalloproteinases-9 (MMP-9) were detected. Result: The total effective rate for the disease was 92.21% in observation group, higher than 77.92% in control group (P<0.05). The total effective rate for traditional Chinese medicine syndrome was 93.51%, higher than 72.73% in control group (P<0.01). After treatment, attack times of angina pectoris in observation group were less than those in control group. The duration time was less than that in control group, and consumption of glyceryl trinitrate was also less than that in control group (P<0.01). Leads number of ST segment down shift, leads number of T wave lowering, and leads number of T wave inverting were all less than those in control group (P<0.01). After treatment, TG abnormal rate in observation group was 10.39%, lower than 22.08% in control group (P<0.05).TC abnormal rate was 9.09, lower than 25.97% in control group (P<0.01). HDL-C abnormal rate was 12.99%, lower than 28.57% in control group (P<0.05).And SAQ scores were higher than those in control group, while heart-blood stasis syndrome score was lower than that in control group (P<0.01). In addition, the levels of hs-CRP, HCY, CysC, CD63 and MMP-9 were lower than those in control group, while level of CD42 b was higher than that in control group after treatment (P<0.01). Conclusion: Guanxin granules can reduce the degree of angina pectoris attacks, regulate lipid metabolism, inhibit the inflammatory response and platelet activation, thereby improving myocardial ischemia, and increasing clinical efficacy in the treatment of stable angina pectoris with heart-blood stasis syndrome关键词：stable angina pectoris;heart-blood stasis syndrome;Guanxin granules;inflammatory reaction;platelet activation
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Fuzheng Jianer paste on non acute phase of recurrent upper respiratory tract infection (RRTI) in children, and investigate its effect on levels of T lymphocyte subsets, immunoglobulin and Th1/Th2 cell factor. Method: One hundred and sixty patients with RRTI at non acute phase were randomly divided into control group and observation group by random number table. Patients in control group received pidotimod oral solution, 400 mg/time, 1 time/day. Zinc gluconate oral solution, 5 mL/time, 2 times/day for 3-6 years old, and 7.5 mL/time, 2 times/day for 7-12 years old; and vitamin A and C were appropriately supplemented. In observation group, the patients received Fuzheng Jianer paste, 5 mL/time, 3 times/day for morning fasting for 3-6 years old, 10 mL/time for 7-12 years old, 2 times/day. The course of treatment was 24 weeks for both groups. An follow-up after the treatment course was conducted for 24 weeks. Attack times of respiratory tract infection, duration time and disease type were recorded during the 48 weeks. Levels of serum immunoglobulin (IgA, IgG and IgM), T lymphocyte subsets (CD3+,CD4+,CD8+,CD4+/CD8+), interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-4 (IL-4) and interleukin-5 (IL-5) were detected. Result: Clinical curative effect was 52.7% in observation group, higher than 32.88% in control group (χ2=5.898, P<0.05). Total effective rate of traditional Chinese medicine (TCM) symptoms was 94.59% in observation group, higher than 78.08% in control group (χ2=8.524, P<0.01). During 48 weeks, the average numbers of attacks (recurrent upper respiratory tract infection, bronchitis, pneumonia) in observation group was less than those in control group, and the average course of disease was also shorter than that in control group (P<0.01). The levels of IgG, IgA and IgM as well as CD3+, CD4+ and CD4+/CD8+ and IL-2 and IFN-γ in observation group were higher than those in control group after treatment (P<0.01), and the levels of CD8+, IL-4 and IL-5 were lower than those in control group (P<0.01). Conclusion: Fuzheng Jianer paste can improve immunity, regulate balance of Th1/Th2, so it can reduce occurrence frequency of respiratory tract infection, shorten attack course, improve cure RRTI rate in the treatment of RRTI with high safety and reliability.关键词：recurrent respiratory tract infection;Fuzheng Jianer paste;immunoglobulin;T lymphocyte subsets;Th1/Th2 cell factor
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发布时间：2024-01-04 - 摘要：Herbal nature is a core component of the theory of traditional Chinese medicine (TCM), and the theory of meridian tropism is an important part of the theory of TCM. The accuracy and pertinence of TCM can be improved by the medicine selection based on the meridian tropism. In order to find the association relationship and the distribution regularity of TCMs with different meridian tropism in the phylogenetic tree, and provide a basis for the interpretation and evaluation of TCM meridian tropism, 2 435 herbs and related 3 044 species of organisms were screened in this study. Association rules mining method and phylogenetic tree constructing method were used to analyze the association relationship and the distribution regularity of the TCMs with different meridian tropism in the phylogenetic tree. The results showed after the frequency of the species of organisms, it turns out several results. In the species of the viridiplantae,there are at most 1 151 species which are attributed to the liver meridian. In the species of the Streptophyta,there are at most 1 109 species which are attributed to the liver meridian. In the species of the Liliopsida,there are at most 110 species which are attributed to the lung meridian.In the process of association rule mining,we can find that the association rules for the same meridian tropism were distributed at the same branch or nearby branch of the tree. For example, Txues were related with kidney, while the Adoxaceae and Rubia have a close relationship with liver. Moreover, colorectal is related with Punica. These results implied that there is a close relationship between the herbal nature and the affinity relationship. TCMs may have the same meridian tropism with the species of the same or similar affinity. This study provided a new index and reference for prediction and evaluation of the nature, selection and compatibility, clinical application of new TCMs.关键词：traditional Chinese medicine;meridian tropism;phylogenetic tree;association rules;evaluation of nature
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发布时间：2024-01-04 - 摘要：Objective: To explore the regularity and characteristics of famous traditional Chinese medicine(TCM) doctors in using Erchentang. Method: Data mining was performed for 341 cases of famous TCM doctors, with Erchentang as the main formula. First, frequency analysis showed symptoms, drugs and syndromes with the frequency of more than 10. Second, factor and cluster analysis was made for frequently used symptoms and drugs. Finally, correlation analysis for frequently used drugs (deducting Erchentang group) and association rule analysis for frequent symptoms (deducting target symptoms of Erchentang) showed meaningful factor group, cluster group, and support and confidence-defined second-order, third-order, fourth-order, five-order correlation results. Result: Meaningful factor group and cluster group were group 9 and group 8, respectively. According to the minimum defined support and confidence, deducting the main formula, there were 10 dual combinations of frequently used drugs, 20 triple combinations and three quadruple combinations. There were 16 triple combinations of drugs for frequent symptoms deducting target symptoms of the main formula and main drugs, eight quadruple combinations and eight quintuple combinations. Conclusion: Erchentang's target symptom types are obstruction of lung by phlegm, dampness stagnancy due to spleen deficiency. Target symptoms are correlated to lung (cough, asthma, sputum), spleen and stomach (abdominal distension, anorexia, loose stools), heart (chest tightness, palpitation, dizziness, insomnia), systemic symptoms (fever, bad cold, body aches), tongue and pulse (pale tongue with whitish and greasy coating, slippery pulse). Commonly used herbs are Cttriexocarpium Rubrum, Pinelliae Rhizoma, Poria, Glycyrrhizae Radix et Rhizoma. Commonly combined drugs herbs include Raphani Semen, Perillae Fructs,Sinapis Semen, Platycodonis Radix, Armeniacae Semen Amarum, and other expectorant, cough suppressant and anti-asthmatic drug, Aurantii Fructus Immaturus and other Qi-activating drugs, Atractylodis Macrocephalae Rhizoma, Dioscoreae Rhizoma and other Qi-invigorating and spleen-tonifying drugs, Zingiberis Rhizoma, Asari Radix et Rhizoma, Ephedrae Herba and other warm-natured drugs. Frequently combined formulas include Sanzi Yangqintang, Liujunzi Tang, Maxing Shigan Tang. Erchentang is a basic phlegm-removing formula, and can be modified into Daotantang, Banxia Fuling Tang and Wenzhong Huatanwan.关键词：Erchentang;data mining;famous traditional Chinese medicine doctor;consilia;factor analysis;clustering analysis;association rules
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发布时间：2024-01-04 - 摘要：Objective: To excavate prescription laws of Tibetan Medicine in treating edema based on Traditional Chinese Medicine Inheritance Support System (TCMISS, V2.5). Method: Prescriptions of Tibetan Medicine for edema were entered into TCMISS to build a Tibetan medicine prescriptions database for treating edema. Software-integrated mutual information improvement method, complex system entropy cluster and unsupervised entropy hierarchical clustering method were adopted to determine the drug use frequency in prescription, commonly used drug pairs and combinations, and analyze Tibetan medicine prescription laws for treating edema. Result: Totally 135 Tibetan medicinal herbs for treating edema were screened out, Among them, Piperis Longi Fructus was the most frequently used, which was followed by Chebulae Fructus, Cartharni Flos, Phyllanthi Fmblicae Fructus and so on. Based on the association rules of a support degree ≥10% and a confidence level ≥0.9, 44 core combinations and 21 new prescriptions were evolved. Conclusion: TCMISS has important value on the study of Tibetan medicine prescription laws due to its functions of data entry, management, inquiry and analysis.关键词：edema;Traditional Chinese Medicine Inheritance Support System (V2.5);data mining;Tibetan medicine prescription
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发布时间：2024-01-04 - 摘要：Lagotis medicinal plants of Tibetan medicine's resources are very abundant, with rich varieties, blood detoxification, heat menstruation effect, and are widely used in Tibetan medicine system. It is a common top grade herb in Tibetan medicines and is used as the main herb in multiple prescriptions. However, the present research reports on Lagotis medicinal plants are less, and the research is mainly concentrated in the pharmacological and chemical studies of L. alutacea, L. brachystachya, L. brevituba, L. ramalana, and L. yunnanensis, while other Lagotis species are rarely reported. Current research shows that Lagotis medicinal plants contain flavonoids, iridoid glycosides, phenylpropanoid glycosides, steroids, terpenoids and other chemical constituents. Modern pharmacological research shows that it has antioxidant, antibacterial, anti-tumor, liver damage resistance, anti-inflammatory, anti gout and other pharmacological activities. Lagotis medicinal plants have very broad prospects for application, but there is little research and development. The literature on chemical constituents and pharmacological effects of Lagotis plant system in recent years was reviewed in this paper, hoping to provide scientific reference for their clinical reasonable application and further development.关键词：Lagotis;chemical constituents;pharmacological action;medicinal plants
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发布时间：2024-01-04 - 摘要：According to the study of ancient and modern literatures,the development and changes of processing methods and effect of Sophorae Flavescentis Radix were mastered.There were rich processing methods in ancient,such as processing with rice-washed water,roasted or stir-fried with wine and vinegar.While,washing and cutting were the main usages in modern.The medicinal parts and effect of Sophorae Flavescentis Radix are also different in modern and ancient.It reminds us that we should strengthen the excavation of the ancient literatures and carry out the study of the correlation between the material basis and its efficacy based on the ancient processing methods.This paper can provide new ideas for the study on processing principle and innovative drugs of Sophorae Flavescentis Radix.关键词：Sophorae Flavescentis Radix;processing method;functional indications;clear away heat and dry dampness;desinsection;diuresis
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发布时间：2024-01-04 - 摘要：Schisandrae is the dry fruit from Schisandra chinesis or S. sphenanthera. The former is called as Schisandrae Chinensis Fructus, and the latter is often called as Schisandrae Sphenantherae Fructus. Schisandrae was first contained in the Shennong Bencao Jing, with a history of over one thousand years as a traditional Chinese medicine in various prescriptions. The efficacy differences between Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus have been recognized by many physicians since Ming dynasty. From the 2000 edition of Chinese Pharmacopoeia, Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus have been introduced sepsrately, and their respective quality control standards have been established until continuous improvement in the 2015 edition of Chinese Pharmacopoeia. The form and origin of Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus were different, with different compositions and functions, but there was a serious phenomenon of mixing use of Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus in clinical application. In order to distinguish Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus, and more reasonably, safely and effectively apply Schisandrae in modern clinical treatment, the recent studies on Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus were collected, retrieved, concluded and summarized in this paper, and the differences in variety identification, chemical components (components of volatile oil, total lignans content and polysaccharide efficacy of Schisandrae Sphenantherae Fructus were better than those in Schisandrae Chinensis Fructus), and pharmacological action (mainly including the effects of Schisandrae on central nervous system, digestive system, and cardiovascular system) were reviewed, hoping to provide a reference for the research, development and clinical application of Schisandrae, fundamentally distinguish Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus not only from the forms and achieve safer and more accurate clinical application.关键词：Schisandrae Sphenantherae Fructus;Schisandrae Chinensis Fructus;species identification;chemical composition;pharmacological effect
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发布时间：2024-01-04
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