最新刊期
卷 23 ， 期 24 ， 2017
- 摘要：Vitiligo is an acquired depigmentation of the skin,incidences of which is 0.5% to 1%.The incidences of vitiligo in young people tend to be more,easy to diagnose and difficult to treat,which affects the appearance.At present,the pathogenesis of vitiligo is not clear,relating to genetic factors,autoimmune,trauma,neurochemical factors,destruction of melanocytes and so on.Now,the animal model of vitiligo is a pathological animal model with western medicine as the main index.Based on the analysis of the clinical characteristics of vitiligo in Chinese and western medicine and a large number of experimental studies on existing animal models,this article forms the following specifications (draft) for the preparation of animal models of vitiligo.关键词：vitiligo;animal models;specifications
- 4
- |
- 4
- |
- 1
发布时间：2024-01-04 - 摘要：Eczema is a common type of allergic and inflammatory skin disease,which is caused by various causes and recurrent attacks.Because the pathogenesis of eczema is uncertain,the present animal model only reflects some characteristics of eczema,and most of these models are acute eczema.Based on the analysis of the characteristics of clinical symptoms of Chinese and western medicine in eczema and the large number of experimental studies on the existing animal models,this article forms the following specifications (draft) for the preparation of animal models of eczema.关键词：eczema;animal models;specifications;drafting;evaluation index
- 2
- |
- 6
- |
- 0
发布时间：2024-01-04 - 摘要：Burn and scald injury is a common symptoms of daily life,which is mostly caused by the high temperature,high fever and other physical burning or high-energy radiation.The burn and scald injury animal model is the pathologic model,which mainly focuses on western medicine.Based on the analysis of the characteristics of clinical symptoms of Chinese and western medicine in burn and scald injury,and the large number of experimental studies on the existing animal models,this article forms the following specifications (draft) for the preparation of burn and scald injury model.关键词：burn;scald;animal models;specification;evaluation index;draft
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Mammary gland hyperplasia is a class of diseases in general closely related to endocrine dysfunction,which is neither inflammation nor tumor of the breast hyperplasia lesions,and its basic pathological changes is the hyperplasia of breast acinar,duct epithelial cells and connective tissue.The mammary gland hyperplasia model is based on endocrine hormone disorders caused by the cause of animal models,but also for the design of animal models with symptoms.Based on the analysis of characteristics of clinical symptoms of Chinese and western medicine,and a large number of animal experiments,preparation specifications (draft) of mammary gland hyperplasia animal model are formed.关键词：mammary gland hyperplasia;animal model;specification;evaluation index;draft
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: In this paper,the rheology and release kinetics of Chuanqi ophthalmic microemulsion in situ gel were investigated. Method: The fluid properties and linear viscoelastic regions of this preparation were investigated by MCR 102 rheometer.The release kinetics of Chuanqi ophthalmic microemulsion in situ gel was evaluated by modified Franz diffusion cell method,the ligustrazine and ligustilide were selected as the indictors,and semi-permeable membrane was used as a barrier. Result: Chuanqi ophthalmic microemulsion in situ gel was a pseudoplastic fluid and it had a linear viscoelastic region.The range of the linear viscoelastic region was 0-302.74 Pa while shear stress as an indicator.The range of the linear viscoelastic region was 0-7.45% while strain as an indicator.At the critical point,the storage modulus(G')=the loss modulus(G")=2 976.60 Pa,critical shear force was 302.74 Pa and critical strain was 7.45%.The release amount of ligustrazine was 33.71 μg within 8 h and its average cumulative release rate was 90.08%,the release kinetics followed Higuchi equation.The release amount of ligustilide was 68.46 μg within 8 h,its release rate was 84.32%,and its release kinetics followed zero-order kinetics equation. Conclusion: Chuanqi ophthalmic microemulsion in situ gel has excellent viscoelasticity,and the deformation is reversible in a certain range.The release kinetics of ligustrazine is result from synergistic effect of its physicochemical properties and matrix skeleton,but the release kinetics of ligustilide is mainly affected by its physicochemical properties.关键词：rheology;linear viscoelastic region;ophthalmic in situ gel;microemulsion;Chuanqi prescription;ligustrazine;ligustilide
- 2
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To recognize the main effective antifungal components against Candida albicans in Artemisia selengensis leaves. Method: The soluble components in water extract of A. selengensis leaves were preliminarily detected and analyzed,and then separated by HPD600 macroporous resin. Based on the knock-out/knock-in model,30% ethanol,50% ethanol,and 70% ethanol water solution were used to obtain different components and with their corresponding negative samples as the research object. Then the anti-fungal components in A. selengensis leaves were recognized by microdilution method, and HPLC-MS was combined for preliminary components analysis. Result: The contents of sugar, protein and other nutrients in water soluble components from water extract of A. selengensis accounted for about 78%, and polyphenols accounted for about 16%. The fraction C (70% ethanol elution) from A. selengensis leaves via HPD600 macroporous resin purification was the main anti-fungal component against C. albicans in a dose-effect dependence manner. Mass spectrometry analysis showed that fraction C was mainly of diterpenoid tanshinones and nalidixic acid compounds, including dehydrogenated tanshinone ⅡA, tanshinone ⅡA and tanshinone Ⅰ, as well as 3 kinds of nalidixic acid derivatives. The above substances were discovered from the A. selengensis for the first time. Conclusion: The main effective anti-fungal components against C. albicans in A. selengensis leaves were studied by constituents knock-out/knock-in strategy, providing the theoretical basis for the further study of monomeric compounds with antimicrobial activity from A. selengensis leaves extract.关键词：Artemisia selengensis;Candida albicans;antifungal activity;knock-out/knock-in strategy;effective contents
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To study on the effect of rice-washed water bleaching products,fried products with bran and raw products of Atractylodis Rhizoma on spleen deficiency rats,and investigate their dryness nature,so as to provide scientific data for clinical medication of Atractylodis Rhizoma. Method: Model of spleen deficiency in rats was prepared by complex method of exogenous dampness disturbing spleen,improper diet and overstrain.SD rats were randomly divided into five groups,8 rats in each group,including the blank group,model group,rice-washed water bleaching Atractylodis Rhizoma group,Atractylodis Rhizoma fried with bran group,crude Atractylodis Rhizoma group.Notes about daily weight,water intake and urine volume were recorded daily.After treatment with 12 days,the gastric emptying velocity and intestinal propulsive ratio were measured by charcoal gavage and spleen index was measured,contents of serum amylase(AMS),D-xylose and gastrin(GAS) were determined. Result: Compared with the model group,each drug treatment group showed weight recovery trend.Fluid consumption in each drug group was higher than that in the model group,and the order was crude Atractylodis Rhizoma group >Atractylodis Rhizoma fried with bran group >rice-washed water bleaching Atractylodis Rhizoma group.The spleen index in each drug group was significantly higher than that in the model group,and Atractylodis Rhizoma fried with bran group and rice-washed water bleaching Atractylodis Rhizoma group were significantly higher than the blank group.Levels of AMS,D-xylose and GAS in each drug group were significantly higher than those in the model group,and Atractylodis Rhizoma fried with bran group and rice-washed water bleaching Atractylodis Rhizoma group were significantly higher than the blank group. Conclusion: Processed products of Atractylodis Rhizoma all have a certain effect on spleen deficiency rats,effects of rice-washed water bleaching products and fried products with bran are significantly better than that of raw products,they all have a good effect of strengthening the spleen and stomach,regulating gastrointestinal function and secretion.Dryness nature is in the order of rice-washed water bleaching products< fried products with bran< raw products.关键词：processing;characteristic pieces of Zhangbang schools;rice-washed water;Atractylodis Rhizoma;dryness nature;pharmacological effects
- 2
- |
- 3
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC specific chromatograms of Phellodendri Chinensis Cortex decoction pieces and charred Phellodendri Chinensis Cortex decoction pieces,and to study on the change law of HPLC specific chromatograms of Phellodendri Chinensis Cortex before and after being processed. Method: HPLC was employed with the ZORBAX SB-C18 column,acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution (phosphoric acid regulated pH to 3.4) was employed as a mobile phase in gradient elution,the detection wavelength was 215 nm,column temperature was at 35℃ and the flow rate was 1.0 mL·min-1. Result: Eleven batches of Phellodendri Chinensis Cortex decoction pieces were marked with sixteen characteristic peaks,and chlorogenic acid,cryptochlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine hydrochloride,palmatine hydrochloride,berberine hydrochloride,obaculatone and obakunone were assigned.Based on the analysis of characteristic peaks,only three common peaks were detected in charred Phellodendri Chinensis Cortex decoction pieces,and the area of these peaks was quite different;in addition,two feature peaks were identified,with the relative high proportion of the characteristic peak being analyzed and verified to be berberrabine,the product of the heat decomposition of berberine hydrochloride. Conclusion: There are significant differences in the specific chromatograms of Phellodendri Chinensis Cortex decoction pieces before and after being processed.The method is accurate and reproducible,which can provide the basis for the quality standard of Phellodendri Chinensis Cortex decoction pieces and charred Phellodendri Chinensis Cortex decoction pieces.关键词：Phellodendri Chinensis Cortex;charred Phellodendri Chinensis Cortex;decoction pieces;specific chromatograms;chlorogenic acid;cryptochlorogenic acid;obakunone;berberine hydrochloride
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Ziziphi Spinosae Semen stir-fried with pig's heart blood,and provide a scientific basis for the inheritance and development of local characteristic processing techniques. Method: Central composite design-response surface methodology was used to optimize the processing technology with overall desirability(OD) of contents of spinosin and jujuboside A as the evaluation index,stir-frying temperature,stir-frying time and the amount of pig's heart blood as factors. Result: The best processing technology was as following:adding 10 g of pig's heart blood into per 100 g of Ziziphi Spinosae Semen,the stir-frying temperature at 131℃,the stir-frying time of 6.1 min;under these conditions,the predicted value of OD was 0.830,its deviation was 1.4% from the actual value. Conclusion: This optimized processing technology is stable and feasible with precise processing parameters,it can provide reference for standardizing special processing technologies of local decoction pieces and their quality standards.关键词：Ziziphi Spinosae Semen;processing technology;spinosin;jujuboside A;central composite design-respone surface methodology
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint of Xinsuning capsules. Method: HPLC was employed,Welch Xtimate C18 column(4.6 mm×250 mm,5 μm) was used with acetonitrile-0.2% formic acid solution (containing 0.2% ammonium hydroxide) as the mobile phase in gradient elution.The flow rate was set at 1.0 mL·min-1 and the detection wavelength was set at 254 nm.Ten batches Xinsuning capsules were collected to assess the similarities,the main peaks in the fingerprint were identified by comparing the retention time of reference substances,and the source and attribution of chemical components in this preparation were analyzed by comparing with single herb. Result: The established fingerprint of Xinsuning capsules was was sensitive and specific.There were 29 common peaks in different batches of Xinsuning capsules and 12 peaks were identified.Compared with the reference fingerprint,the fingerprint similarities of ten batches of Xinsuning capsules were more than 0.98.A comparative analysis showed the contribution of Coptidis Rhizoma and Aurantii Fructus Immaturus was the highest,their contribution rates were 0.21. Conclusion: The analytical method of HPLC fingerprint is stable and reliable with fine repeatability,which provides a reference for study on material basis and quality control of Xinsuning capsules.关键词：Xinsuning capsules;fingerprint;quality control;similarity
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Pesticide residue is a major exogenous pollutant of traditional Chinese medicine (TCM), which seriously affects the safety and efficacy of TCM, therefore, it is necessary to systematically investigate the pesticide residue and establish data files of TCM. According to the test results of pesticide residues from 11 kinds of TCM samples collected from the fourth Chinese Materia Medicine Resource Survey in 2012-2014, and 2016. In the analysis results of a total of 542 batches of TCM samples, pesticide residues were detected in 263 batches, of which only 5 batches had exceeded content of the pesticide residues accounting for about 0.92% of the total TCM samples, and the pesticide residues content of the remaining TCM samples was less. In the statistics analysis of pesticide residues from different species, different medicinal parts and different producing areas, there were some common low-toxic pesticides and also some high-toxic pesticides which have been banned for using in some TCM samples. This phenomena shall be received great concern by the relevant departments. This paper would put forward some reasonable recommendations and some pesticide varieties which need to be monitored according to the analysis results, with a view to providing some effective data, establishing monitoring and management systems and perfecting the standard on pesticide residues of TCM samples for "Chinese Pharmacopoeia".关键词：traditional Chinese medicine;pesticide residue;pesticide varieties;key monitoring;standard formulation
- 2
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To identify the traits of 58 batches of Platycodonis Radix samples from 10 provinces and cities, determine the content of root extract and platycodin D in the samples, establish and compare their High Performance Liquid Chromatography (HPLC) fingerprints. Method: The content of platycodin D and the root extract in Platycodonis Radix was determined according to the determination method in Chinese Pharmacopoeia. HPLC fingerprints of Platycodonis Radix were determined by High Performance Liquid Chromatography-Evaporative Lightscattering Detector (HPLC-ELSD) method and gradient elution flowing form. Result: In these 58 batches of sample, the surface color in 2 batches, size in 7 batches, and section characteristics in 11 batches did not comply with the requirements in pharmacopoeia. The content of platycodin D and root extract in 58 batches of Platycodonis Radix from 10 provinces or cities was determined, and the total failure rate of the root extract of Platycodonis Radix sample and platycodin D was 36.20%. A total of 17 common peaks were found in 58 batches of Platycodonis Radix. The content of extract and platycodin D, as well as HPLC fingerprints in Platycodonis Radix samples from Sichuan province showed high similarity, and these above three aspects showed low similarity in the samples from Chongqing and Hunan province. Cluster analysis and comparative study of the traits, root extract content, platycodin D content and HPLC fingerprints were performed on 58 batches of Platycodonis Radix from different habitats. The results showed that there was no positive correlation between the content of extract, platycodin D and HPLC fingerprint similarity, but the difference in content of extract and other components in the samples was related to the characteristics of the samples (skin). Conclusion: The identified traits, the determined content of platycodin D, root extract and the established HPLC fingerprints can provide a more comprehensive, authentic and representative reference for screening of high quality Platycodonis Radix, revision of the Chinese Pharmacopoeia and quality evaluation of Platycodonis Radix.关键词：Platycodonis Radix;traits;root extract;platycodin D;HPLC fingerprint
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To further investigate the effective components of Delphinium grandiflorum, enrich the natural resource database of diterpenoid alkaloids, and provide more abundant chemical component resources. Method: The constituents were isolated by column chromatography of silica gel, MCI gel, ODS and Sephadex LH-20, and their structures were determined on the basis of MS, NMR spectroscopic analysis (1H,13C-NMR, MS), physicochemical properties and comparison with literature data. Result: Nineteen diterpenoid alkaloids compounds were isolated from the 95% ethanol extract of the whole herbs of D. grandiflorum. Their structures were identified as olivimine (1), hohenackeridine (2), 14-O-methyldelphinifoline (3), N-deethyldelphatine (4), leucostine (5), tuguaconitine (6), acetylbrowniine (7), browniine (8), 16-demethyldelsoline (9), demethylenedelcoine (10), 14-dehydrobrowniine (11), septentriodine (12), delsoline (13), delphatine (14), lycoctonine (15), delcosine (16), methyllycaconitine (17), delavaine A (18) and delavaine B (19). Conclusion: Compounds 1-5 were isolated from this genus for the first time, and compounds 6-12 were obtained from this species for the first time.关键词：Delphinium grandiflorum;diterpenoid alkaloids;chemical constituents;structure elucidation
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To determine the content of acteoside in tuberous roots and leaves of different cultivars of Rehmannia glutinosa during their different growth periods at genuine producing areas, and investigate the changes in dynamic accumulation. Method: The acteoside contents from tuberous roots and leaves in 85-5, Beijing-1, Qinhuai, Jingjiu and Baixuan that were cultivated in 2015 and 2016 at Wenxian county were investigated by HPLC method during different harvest periods. Furthermore, acteoside content was detected in tuberous roots and leaves of 85-5 after shading treatments in 2014. Result: There were great differences of acteoside content in tuberous roots and leaves among the different cultivars of R. glutinosa. Acteoside contents were 0.023%-0.620% and 0.018%-0.796% in tuberous roots of Baixuan and Qinhuai respectively, which were higher than that of other cultivars. However, 85-5 and Beijing-1 had higher content of acteoside in leaves, ranging from 1.955%-5.968% and 0.681%-5.941%. The contents of acteoside were higher in unfolded leaves and senescing leaves than tender leaves. Shading improved its content in tuberous roots and restrained the accumulation of acteoside in leaves. Conclusion: The results provide the experimental materials and basis for breeding the high-acteoside-content R. glutinosa cultivars, and lay foundations for studying the regulation mechanisms of acteoside biosynthesis in R. glutinosa.关键词：Rehmannia glutinosa;acteoside;tuberous root;leaf;shading;different cultivars
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprints and spectrum-effect relationships of different batches of Filifolium sibiricum, clarify the contribution of the chemical constituents represented by the common peaks to the antimicrobial efficacy, and reveal the pharmacological basis. Method: Fingerprint was established by HPLC method with acetonitrile-PBS (0.1 mol·L-1 sodium dihydrogen phosphate and 2% acetic acid mixed 1:1) as the mobile phase for gradient elution and the detection wavelength was 360 nm. The inhibitory effects of different samples of F. sibiricum on Staphylococcus aureus, Escherichia coli, methicillin resistant S. aureus, Bacillus paratyphoid and S. albus were studied to reflect their antibacterial activity. Spectrum-effect relationship was revealed by multivariate linear regression and grey relational analysis method. Result: The result of multiple linear regression analysis showed that five antibacterial group experiments (S. aureus, E. coli, methicillin resistant S. aureus, B. paratyphoid and S. albus) were developed respectively and nine peaks per group were correlated with antibacterial activities. The results of gray correlation analysis indicated that isorhamnetin-3-O-rutinoside, isoorientin and isovitexin were correlated with methicillin resistant S. aureus; E. coli and S. aureus were correlated with isorhamnetin-3-O-β-D-glucoside, scopoletin, myricetin, galuteolin, eriocitrin and isoquercitrin; S. albus was correlated with luteolin and quercetin 3-O-rutinoside-7-O-glucoside; and B. paratyphoid was correlated with eriocitrin, mangiferin, myricetin, rutin, scopoletin and isorhamnetin-3-O-β-D-glucoside. The above inhibitory correlation degrees were all more than 0.8. Conclusion: The established HPLC fingerprint method was simple and reproducible.关键词：Filifolium sibiricum;bacterial drug resistance;fingerprint;antibacterial activity;spectrum-effect relationship
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To establish an Ultra-High Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry (UPLC-MS/MS) method for determination of ten flavonoids in ginkgo leaves extract, and provide scientific basis for its quality control. Method: The analysis was performed on a ACQUITYTM UPLC BEH C18 (2.1 mm×50 mm, 1.7 μm) with 0.1% formic acid solution-acetonitrile as the mobile phase for gradient elution (0-5 min, 90%-70%A; 5-8 min, 70%-50%A). The column temperature was 30℃ and the flow rate was set at 0.3 mL·min-1. Result: All 10 flavonoids showed good linear relationship (0.999 5-1) with the peak area within their tested ranges (0.01-85.00 mg·L-1). The average recoveries of ten flavonoids were 96.10%, 96.77%, 97.61%, 97.83%, 100.49%, 99.28%, 98.29%, 101.23%, 101.28% and 102.25% with RSD values below 5.0%. Total content of flavonoids in different batches of ginkgo leaves extract ranged from 89.29 mg·g-1 to 114.18 mg·g-1. The content of flavonoid glycosides accounted for a large proportion in the total flavonoids, while the contents of flavonoid aglycone such as luteolin and taroxinin were lower. The different flavonoids also had significant difference in content, for example, the contents of diglycoside rutin and quercetin-3-O-β-D-pyran glucose (4→1)-α-L-pyran rhamnoside were higher than the content of monoglycoside (isoquercitrin, quercetin). Conclusion: The method can be used for qualitative and quantitative analysis of flavonoids in ginkgo leaves extract.关键词：Ginkgo leaves extract;UPLC-MS/MS;quality control
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC fingerprint of water decoction of Moslae Herba for quality evaluation, and lay the foundation for the quality control of Xinjia Xiangruyin granules. Method: The chromatographic fingerprints of 10 batches of water decoction of Moslae Herba from different places of origin were obtained on Phenomenex Luna C18 (4.6 mm×250 mm, 5 μm), with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. The column temperature was maintained at 40℃ at a flow rate of 1 mL·min-1, and the detection wavelength was 269 nm. 10 batches of fingerprints were imported into Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012.130723) for chromatographic peak matching. With caffeic acid as the reference peak, the midpoint method was used to establish the reference fingerprint, which was then compared with the fingerprint of 10 batches of Moslae Herba. Result: The HPLC fingerprint of Moslae Herba showed 14 common peaks. The similarities of 10 peaks from different origins were all more than 0.90.Then the analysis results on relative peak areas, showed less differences in the quality of Moslae Herba from 10 origins. Conclusion: With good reproducibility and feasibility, this method can be used as the means to assess the quality of Moslae Herba and provides reference for preparing the quality standard of Xinjia Xiangruyin granules.关键词：Moslae Herba;water decoction;HPLC fingerprint
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the metabolites of Inula cappa extract in rat feces after oral administration. Method: UPLC-Q-TOF-MS/MS was used for analyzing the metabolites of I. cappa extract in rat feces after oral administration at a dose of 100 g·kg-1.Separation of the metabolites was performed on an RRHD Eclipse Plus C18 column (2.1 mm×100 mm,1.8 μm) with the mobile phase of 0.1% formic acid-0.1% formic acid acetonitrile solution for gradient elution,and the samples were treated by electron spray negative ionization mode (ESI-).Then the data were processed with metabolite tools softwares to identify the chemical structure of metabolites. Result: M8 (1,3-O-dicaffeoylquinic acid) and 21 other metabolites were identified,including M1-M4(hydrogenation of caffeoylquinic acid),M5(sulfation of dihydrocaffeic acid),M6-M7(methylation of caffeoylquinic acid) and so on. Conclusion: Hydrogenation and methylation are the major metabolic pathways of I. cappa extract in rat feces.关键词：Inula cappa;extract;caffeoylquinic acids;metabolites;UPLC-Q-TOF-MS/MS;chlorogenic acid
- 2
- |
- 3
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Shuangshen Xionglian (SSXL) granule on atherosclerotic inflammation in ApoE-/- mice with high-fat diet. Method: C57BL/6 mice were used as normal control group (BLA). ApoE-/- mice were randomly divided into 4 groups:model group with atherosclerosis (MOD), probucol treatment group (PRO, 216 mg·kg-1), SSXL high dose group (SSXL-H, 1.6 g·kg-1) and SSXL low dose group (SSXL-L, 0.8 g·kg-1). After 2 weeks of high-fat diet, blood lipids were measured by biochemical method and ApoE-/- mice were treated with different drugs. Blood lipids were measured again after 8-week treatment. Aortic intima thicknesses in ApoE-/- mice were analyzed by ultrasonic methods. Aortic plaque areas were observed by oil red O staining and ratio of plaque area/intravascular area was calculated. The expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1α, IL-10 and monocyte chemoattractant protein (MCP)-1 in serum were detected by flow type high throughput multi-factor detection technique. Result: SSXL granule could decrease aortic intima thicknesses in ApoE-/- mice, reduce ratio of plaque area/intravascular area, inhibit the expression of TNF-α and MCP-1, but with no significant effects on changes of blood lipids. Conclusion: SSXL granule had obvious anti-atherosclerotic effect by suppressing inflammation but not depending on blood lipids level.关键词：Shuangshen Xionglian granule;atherosclerosis;inflammation;ApoE-/- mice
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To use Shenqi Jieyu formula with the power of tonifying heart and spleen to treat postpartum depression (PPD) model animals, and observe the changes in relevant prefrontal cortex, hippocampus 5-serotonin (5-HT) and norepinephrine (NE) and their receptors, in order to explore the intervening mechanism of traditional Chinese medicine(TCM) in in tonifying heart and spleen. Method: Female SD rats, 2 to 3 months old, were randomly divided into normal (N) group, sham operation (SO) group, model (M) group, TCM group, and western medicine (WM) group. All rats were observed respectively at 1, 2, 4, 6 weeks after preparation. The PPD model was established through sudden withdrawal of hormones after continuous injection with estrogen and progesterone. Then, the rats were given pharmacological intervention. The rats' materials were collected at the prescribed time points. Their behaviors were tested at each time points. Then the contents of 5-HT, NE were detected by HPLC, and the expressions of 5-HT1AR, α2AR were detected by IHC in prefrontal cortex and hippocampus. Result: Compared with N and SO, contents of 5-HT and NE in prefrontal cortex and hippocampus in M at each time point were lower (P<0.01). Compared with M, contents of 5-HT, NE in prefrontal cortex in TCM, WM at each time point were all increased, except for at 2, 4, 6 weeks in TCM (P<0.05, P<0.01), with the same outputs in hippocampus in TCM and WM at each time point. Compared with M, levels of 5-HT1AR in prefrontal cortex and hippocampus in N and SO at each time point were enhanced (P<0.01), while levels of α2AR in above places and at the time points were attenuated (P<0.01). Expressions of 5-HT1AR in prefrontal cortex in TCM at 2, 4, 6 weeks and in hippocampus at each time point were all higher than M (P<0.01). Compared with M, expressions of α2AR in prefrontal cortex in TCM at 2, 4, 6 weeks and WM at 4, 6 weeks, and in hippocampus in TCM at 4, 6 weeks and WM 2, 4, 6 weeks were decreased (P<0.05, P<0.01). Conclusion: Shenqi Jieyu formula could significantly promote contents of 5-HT and NE in PPD rats' brain, and effectively regulate the abnormal expressions of their receptors. The balance adjustment for the above neurotransmitters and their receptors might play a very important role for this formula on treating PPD.关键词：postpartum depression;prefrontal cortex;hippocampus;monoamine neurotransmitters and their receptors;Shenqi Jieyu formula
- 2
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Yangyin Qingfei decoction in regulating T cells Notch signal receptor 1 (Notch1) and Notch signal ligand 1 (Jagged1) in rats with radiation pneumonia, in order to explore its mechanism for preventing and treating radiation pneumonia. Method: Forty male and female SD rats were randomly divided into normal group, model group, hormone group and combination group. The dosage was 16 Gy. On the first day after irradiation, the rats in each group were given the corresponding medicine. At the 4th week after irradiation, the rats were put to death, and their lungs were weighed. The lung tissues were detected by hematoxylin-eosin (HE) staining, Masson staining and Notch1 immunohistochemical staining. Immunohistochemistry was used to extract T cells, and flow cytometry was used to detect Notch1 percentage. The changes in lung function and the expressions of Notch1 and Jagged1 were observed 4 weeks later after irradiation. Result: At the 4th week after radiotherapy, there were significant differences in lung coefficient between groups, and the lung coefficient of normal group was the closest to combined treatment group. Compared with model group, Yangyin Qingfei decoction combined with prednisolone can significantly reduce the proportion of Notch1 T cells (P<0.05), the expressions of Notch1, NICD and Jagged1 were down-regulated significantly (P<0.05), and Notch1 and Jagged1 mRNA expressions were significantly decreased (P<0.05). Conclusion: Yangyin Qingfei decoction can significantly reduce Notch1 and Jagged1 expressions of radioactive pneumonia rats, and may be one of prevention and treatment mechanisms for the occurrence and development of radiation pneumonia.关键词：radiation pneumonia;Yangyin Qingfei decoction;T cell;Notch1;Jagged1
- 4
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic efficacy of Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma on nude mice with bone metastasis of breast cancer and its mechanism. Method: Nude mice were randomly divided into normal group, model group, zoledronate group and Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group. Except for normal group, MDA-MB-231BO cells were injected into the left ventricle to create the model of bone metastasis of breast cancer. Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group nude mice were orally administered with Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma. Normal group, model group and zoledronate group nude mice were orally administered with physiological saline, and zoledronate was injected into the abdominal cavity in zoledronate group. The degree of bone metastases was evaluated. Bone mineral density (BMD), bone volume (BV), range of interests (ROI) and total volume (TV) were evaluated by micro-CT. Osteoclasts in bone metastasis were evaluated by tartrate resistant acid phosphatase dye (TRAP). Transforming growth factor-β1(TGF-β1), drosophila mothers against decapentaplegic protein 3 (Smad3), Smad4, Glioma-associated oncogene homologue 2 (Gli2) and Parathyroid hormone-related protein (PTHrP) in bone metastasis tissues were evaluated by Western blot. Result: The degree of bone metastases, BMD and BV/TV in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group were higher than those of model group (P<0.01). The number of TRAP (+) cells in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group was lower than that of model group (P<0.01). The expression of TGF-β1, Smad3, Gli2 and PHTrP protein expressions of bone metastases tissues in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group were lower than those of model group (P<0.01), and the expression of Smad4 in Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma group was higher than that of model group (P<0.05). Conclusion: Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma has a therapeutic effect on soluble osseous injury in nude mice with bone metastasis of breast cancer, and the mechanisms of action may be that Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma could up-regulate Smad4, down-regulate TGF-β1, Smad3, Gli2 and PTHrP, and regulate TGF-β1/Smads/Gli2/PTHrP signaling pathway.关键词：bone metastasis of breast cancer;Aconiti Lateralis Radix Praeparata-Atractylodis Macrocephalae Rhizoma;TGF-β1/Smads/Gli2/PTHrP signaling pathway;warming kidney and strengthening spleen
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Phyllodii Pulchelli Radix on carbon tetrachloride CCl4-induced hepatic fibrosis of cynomolgus monkeys and the underling mechanism. Method: The 30 male cynomolgus monkeys were randomly divided into 5 groups, namely normal group, model group, colchicine group (0.08 mg·kg-1), Phyllodii Pulchelli Radix high and low dose groups (4.0, 2.4 g·kg-1), with 6 monkeys in each group. Except the monkeys in normal group, CCl4-induced hepatic fibrosis models were established by intragastrical administration of 40% carbon tetrachloride (CCl4) olive oil mixture at dose of 1.6 mL·kg-1 in other animals, 2 times a week. From week 4, intragastrical administration was given for 12 weeks in various treatment groups. They were weighed every 4 weeks, and blood was taken to detect liver function. The levels of hyaluronic acid (HA), laminin(LN), metal matrix proteinase-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in serum were detected by using ELISA. At week 8 and week 16, livers of all animals were detected by B ultrasound, then aspiration biopsy was conducted under B ultrasound. Hematoxylin-eosin (HE) staining and Van-Gienson staining were conducted on the aspiration biopsy samples, and transforming growth factor-β1 (TGF-β1) and smooth muscle actin-α (α-SMA) in liver were also detected in the samples by Western blot. Result: After administration of CCl4 for 8 weeks, the levels of alanine amino transferase (ALT), aspartate amino transferase (AST), total bilirubin (TBIL), alkaline phosphatase(ALP), HA and LN in serum were increased in model group, colchicines group, and Phyllodii Pulchelli Radix high and low dose groups as compared with control group and before modeling before, while total protein (TP) and albumin (ALB) in serum were decreased significantly (P<0.05, P<0.01). After modeling for 12 weeks, TIMP-1 was increased significantly in model group and all treatment groups as compared with the normal group (P<0.05). After modeling for 16 weeks, the strong echo was enhanced in liver of model group in detecting by B ultrosound, and HE staining showed hepatocyte necrosis and fibroblast proliferation; Van-Gieson staining showed that the content of collagen fiber was increased, and the expression levels of TGF-β1 and α-SMA in liver were increased significantly in model group (P<0.05). After administration of colchicines or Phyllodii Pulchelli Radix for 8 weeks, the levels of AST, ALT, TBIL, ALP and HA in serum were decreased significantly in colchicines group and high dose Phyllodii Pulchelli Radix group as compared with model group, while the level of TP was increased differently (P<0.05, P<0.01). After administration of colchicines or Phyllodii Pulchelli Radix for 12 weeks, the levels of LN and TIMP-1 were decreased significantly in colchicines group and high dose Phyllodii Pulchelli Radix group (P<0.05), while the level of ALB was increased (P<0.05). The results from B ultrasound and pathological examination showed that the fibrosis in liver was relieved in colchicines group and high dose Phyllodii Pulchelli Radix group, and the levels of TGF-β1 and α-SMA in liver were also decreased (P<0.05). Conclusion: Administration of Phyllodii Pulchelli Radix at dose of 4.0 g·kg-1 showed significant efficacy for liver fibrosis of cynomolgus monkeys, could obviously improve the liver function and reduce the degree of fibrosis. The underlying mechanism may be correlated with reduction of liver cell damage and prevention of collagen fiber formation.关键词：Phyllodii Pulchelli Radix;pharmacodynamics;hepatic fibrosis of cynomolgusmonkey;CCl4;liver function
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect and possible mechanism of Qixian Tongluo decoction on endogenous neural stem cells regeneration after cerebral ischemia in rats. Method: The focal cerebral ischemia model was established by the method of middle cerebral artery occlusion (MCAO). A total of 160 rats were randomly divided into normal group, sham operation group, model group, piracetam group (piracetam tablets intragastric administration), and Qixian Tongluo decoction group (Qixian Tongluo decoction intragastric administration). 5-Bromodeoxyuridine (BrdU) was injected intraperitoneally to label proliferating cells in brain; at day 3, 7, 14 and 28 after MCAO modeling, the neonatal neurons and astrocytes in the ischemic hippocampus were detected by immunofluorescence BrdU/neuron nuclear antigen (NeuN) and BrdU/glial fibrillary acidic protein (GFAP) double staining, and then the positive rate of double labeling cells was calculated. In addition, the expression level of brain derived neurotrophic factor (BDNF) in the ischemic brain was detected by immunohistochemistry. Result: The percentages of BrdU/NeuN and BrdU/GFAP double-labeled positive cells in Qixian Tongluo decoction group and piracetam group were all higher than those in the model group at day 7, 14 and 28 after cerebral ischemia (P<0.01). The percentages of BrdU/NeuN positive cells in Qixian Tongluo decoction group were all higher than those in piracetam group at different time points (P<0.05); however, the percentage of BrdU/GFAP double-labeled positive cells in Qixian Tongluo decoction group was higher than that of piracetam group only at day 7 after MCAO (P<0.05), and then the percentages were decreased in both groups. As compared with the model group, the expression levels of BDNF in the ischemic brain of Qixian Tongluo decoction group and piracetam group were all higher at different time points after cerebral ischemia (P<0.01). The expression level of BDNF in Qixian Tongluo decoction group reached the peak at day 3 after MCAO, and then declined at the following time points, but still higher than that in the piracetam group (P<0.05). Conclusion: Qixian Tongluo decoction can promote endogenous neural stem cells regeneration in rats with cerebral ischemia especially during convalescence and sequelae stages, and the early activation of astrocytes and upregulation of BDNF in ischemia brain may be one of its mechanisms.关键词：cerebral ischemia;Qixian Tongluo decoction;neural regeneration;brain derived neurotrophic factor;astrocyte activation;rat
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To study the effects of Dihuang Yinzi decoction on bone strength and femur pathological morphology of ovariectomized rats, to further explore its mechanism. Method: Cutting off ovaris on both sides made rat model of osteoporosis, all animals were randomly separated into sham operation group, model control group, Dihuang Yinzi decoction high, medium, low dose group, western medicine control group(estradiol valerate tablets), with 10 rats in each group. The rats were gavaged with corresponding drugs for 4 weeks. And weight method was monitor the weight body of each rats. The general statu of the rats was evalued, the contents of Ca and P was used to check by colorimetric analysis, the contents of BGP and trACP were used to check by ELISA, bone mineral density(BMD) was used to obtain data by X ray dual energy absorptiometer, maximum load and fixed elongation displacement was used to obtain data by AG-IX biomechanical universal testing machine, the morphological changes of the femur was used to observe by HE staining. Result: Compared with those of sham operation group, the BMD,Ca,P,BGP,maximum load and fixed elongation displacement were all decreased obviously (P<0.05), but the weight body and trACP activity were both markedly increased (P<0.05), as well as, trabecular bone structure was incomplete,missing and broken seriously. Compared with those of model control group, the BMD,Ca,P,BGP,maximum load and fixed elongation displacement were all raised sharply in dihuangyinzi decoction treatment group, the P content was increased sharply in dihuangyinzi decoction high,middle dose group(P<0.05), but the weight body and trACP activity were both significantly slowed in Dihuang Yinzi decoction high dose group (P<0.05), meantime, bone trabecula was arranged regularly, bone trabecula was increased in number and widthin Dihuang Yinzi decoction high dose group. Conclusion: Dihuang Yinzi could improve bone density and inhibit bone formation, reduce bone absorption in rats with postmenopausal osteoporosis, and lead to a positive effect on the treatment of postmenopausal abnormal bone metabolism.关键词：Dihuang Yinzi decoction;ovariectomized rats;bone strength;calcium and phosphorus metabolism
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of 17-methoxyl-7-hydroxyl-benzofuran chalcone (YLSC) postconditioning on myocardial ischemia reperfusion (I/R) injury in isolated rat hearts and investigate its related signaling pathway. Method: Total 60 male SD rats were randomly divided into 5 groups:normal group, model group, YLSC group (5 μmol·L-1),AG490 group (2 μmol·L-1) and YLSC+inhibitor group (YLSC 5 μmol·L-1+AG490 2 μmol·L-1), n=12 in each group. The isolated rat hearts were subjected to 30 min ischemia+120 min reperfusion to establish the I/R model. AG490 or YLSC was given 10 min before reperfusion. The heart rate (HR), the left ventricular end diastolic pressure (LVEDP) and the maximum change rate of left ventricular pressure (±dp/dtmax) were recorded, and the level of lactic dehydrogenase (LDH) in coronary effluent was detected. The rats were sacrificed after reperfusion, and their infarct sizes were measured by triphenyltetrazolium chloride(TTC) staining. Phosphorylated janus kinase 2 (JAK2), phosphorylated signal transducer and activator of transcription 3 (STAT3), B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) protein expression levels in myocardium were measured by Western blot. Result: As compared with the model group, YLSC postconditioning obviously ameliorated cardiac function, decreased myocardial infarct sizes and LDH level, reduced apoptotic rate and Bax expression levels, and increased p-JAK2, p-STAT3 and Bcl-2 levels (P<0.05, P<0.01). Combination of YLSC and AG490 could attenuate the hemodynamic improvement effect, increase the infarct sizes, LDH level and Bax expression level, and down-regulate p-JAK2, p-STAT3 and Bcl-2 expression levels as compared with YLSC group (P<0.05, P<0.01). Conclusion: YLSC postconditioning could effectively protect against myocardial I/R injury in isolated rat hearts, and its mechanism may be associated with activation of JAK2/STAT3 signaling pathway.关键词：17-methoxyl-7-hydroxyl-benzofuran chalcone (YLSC);ischemia/reperfusion;postconditioning;janus kinase 2/signal transducer and activator of transcription 3
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To explore whether the renal protective effect of Tangshen'an is related to the activation of Nrf2/ARE pathway, the role of anti-oxidative stress and reduction of inflammatory response. Method: Male Wistar rats were randomly divided into 6 groups:normal group, model group, Tangshen'an low, middle and high dose groups, and irbesartan group. Their drug-containing serum was prepared from each group. The serum of normal group was added into mesangial cells cultured in normal concentration of glucose, and the serum from other 5 groups was respectively added into the mesangial cells cultured in high concentration of glucose. The mRNA and protein expression levels of nuclear factor E2-related factor 2 (Nrf2) and its downstream factor γ-glutamylcysteinesynthetase(γ-GCS) were detected by Real-time polymerase chain reaction (Real-time PCR) and Western blot respectively. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the supernatant of each group were detected by ELISA. Result: As compared with the model group, the mRNA and protein expression levels of Nrf2 and γ-GCS were significantly up-regulated (P<0.01), and the contents of IL-6 and TNF-α were decreased (P<0.01) in various treatment groups. Conclusion: Tangshen'an can activate Nrf2/ARE pathway, decrease oxidative stress, reduce inflammation, and eventually achieve the role of renal protection.关键词：Tangshen'an;diabetic kidney disease;Nrf2/ARE pathway;inflammatory factor
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To conduct a comparative study on the toxic reaction of Niuhuang Jiedu tablets containing Calculus Bovis (Niuhuang) from different sources in mice, in order to explore the mechanism of chronic arsenic poisoning clinically caused by Niuhuang Jiedu tablets. Method: Test sample Niuhuang Jiedu tablets were respectively made by using natural Calculus Bovis, Bovis Calculus Artificial, and Bovis Calculus Sativus, with no Calculus Bovis element and no Realgar element, according to Chinese Pharmacopoeia (Version 2015). The same dose of test sample single Realgar was used for control. Each Niuhuang Jiedu tablets group was divided into low, middle and high-dose groups, ICR mice were intragastrically administered with drugs for 28 days, then the toxic reaction was observed during the period of drug administration. After drug administration, these mice were put to death. Their blood samples were collected to test liver and kidney function, and tissues of heart, liver, spleen, lung and kidney were collected for histopathological examination and ultrastructure observation under electronic microscopy. Result: In each test group, no toxic reaction was observed during drug administration. No gross lesion was observed in viscera and skins after the mice were put to death. And no clear toxic effect of Realgar on liver and kidney functions was found in blood biochemistry examination. In each Niuhuang Jiedu tablets group, no change in histopathology and ultrastructure under electronic microscopy was found. But in high-dose single Realgar group, the histopathological examination showed that in hepatic lobule, hepatocytes became enlarged, and glycogen accumulated in cytoplasma according to hematoxylin-eosin (HE) staining, which were further confirmed by periodic acid Schiff reaction (PAS) staining. Ultrastructure under electronic microscopy showed in hepatocytes focal cytoplasm dissolutions, and glycogen granules were found inside. Conclusion: No obvious toxic reaction was found in ICR mice after administration with Niuhuang Jiedu tablets containing bovis calculus from different sources for 28 days, but the same dose of single Realgar could cause liver toxicity. It is suggested that other ingredients in Niuhuang Jiedu tablets combined with Realgar can significantly reduce the concentrations of soluble arsenic and arsenic valence state, thus reducing the toxicity of Realgar.关键词：Niuhuang Jiedu tablets;compatibility of medicines;chronic arsenic poisoning;histopathology;ultrastructure
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Wuwei Xiaoke decoction in treatment of type 2 diabetes with Qi and Yin deficiency. Method: A total of 128 patients with type 2 diabetes were selected and randomly divided into two groups:treatment group (64 cases) and control group (64 cases). Two groups were both treated with the basic therapy; the treatment group was additionally given Wuwei Xiaoke decoction, and control group was additionally given placebo. Clinical symptoms, sign changes, fasting blood glucose (FBG), postprandial 2 h blood sugar (P2 hBG), glycosylated hemoglobin (HbA1c), fasting insulin level (FINS), insulin resistance index (HOMA-IR), traditional Chinese medicine (TCM) syndrome score of two groups were observed after treatment. Result: Two groups were evenly distributed, with a good baseline status and strong comparability. In the aspect of TCM syndrome scores, treatment group was significantly better than control group in the alleviation of clinical symptoms (P<0.05). The total clinical effective rate was 93.55% in treatment group was significantly higher than control group (P<0.05). After treatment, two groups showed alleviations in FBG and P2 hBG, HbA1c, FINS and HOMA-IR, particularly the treatment (P<0.05). Conclusion: Wuwei Xiaoke decoction has a significant efficacy and safety in treating type 2 diabetes with Qi and Yin deficiency syndrome, and thus is worth clinical popularization.关键词：type 2 diabetes;Wuwei Xiaoke decoction;weak viscera;Qi and Yin deficiency
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Wenjing Tongluo decoction combined with acupuncture on the expression of serum Wnt-3α, β-catenin and bone morphogenetic proteins-2 (BMP-2) of patients with wind cold dampness arthritis. Method: The 112 patients with wind cold dampness type rheumatoid arthritis (RA) treated in our hospital from February 2015 to November 2016, were randomly divided into observation group and control group, 56 cases in each group. The observation group was treated with Wenjing Tongluo Tang combined with acupuncture, while the control group was treated with celecoxib combined with leflunomide. Then the clinical efficacy, clinical symptom scores of traditional Chinese medicine (TCM), erythrocyte sedimentation rate (ESR), C reactive protein (CRP) and rheumatoid factor (RF) changes and the changes of serum Wnt-3α, β-catenin and BMP-2 levels before and after the treatment were compared between two groups. Result: The total effective rate was 94.64% in observation group, significantly higher than 74.00% in control group (P<0.01). After the treatment, the clinical symptom scores of TCM were significantly decreased as compared with those before the treatment in both groups (P<0.01), and the decrease was more obvious in observation group (P<0.01). ESR, CRP and RF levels were significantly decreased after the treatment in both groups (P<0.01), and the decrease was more obvious in observation group (P<0.05). The serum levels of Wnt-3α, β-catenin and BMP-2 were significantly decreased after treatment in observation group (P<0.01), but there was no significant difference in the control group before and after the treatment. Conclusion: Wenjing Tongluo decoction combined with acupuncture has remarkable curative effect in the treatment of wind cold dampness rheumatoid arthritis, and it may play a role in relieving joint pain and improving joint function by down-regulating Wnt-3α, β-catenin, and BMP-2 expression levels.关键词：Wenjing Tongluo decoction;acupuncture;wind cold dampness type rheumatoid arthritis;Wnt signaling pathway;bone morphogenetic protein-2
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To explore the therapeutic effect and safety of Canshitang combined with colchicine in treatment of the gouty arthritis syndrome of accumulated dampness-heat. Method: A total of 258 patients with gouty arthritis syndrome of accumulated dampness-heat were randomly divided into the traditional Chinese medicine(TCM) group, western medicine group and the integrated Chinese and western medicine group, with 86 cases in each group. The TCM group was treated with Canshitang recipe by oral administration; the colchicine was applied by oral administration in the western medicine group, and Canshitang combined with colchicine was adopted in integrated Chinese and western medicine group, with a course of 14 d in all three groups. The changes in clinical symptoms, serum uric acid (SUA), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and interleukin-6 (IL-6) levels were observed before and after treatment, and the total effective rate, incidence of adverse reactions and 3-month recurrence rate were compared among these three groups. Result: The 3 cases were removed in TCM group in the treatment; 2 cases were removed in western medicine group, and 5 cases were removed in integrated Chinese and western medicine group. The total effective rate was 93.8% in integrated Chinese and western medicine group, better than 81.9% in TCM group and 83.3% in western medicine group (P<0.05); there was no significant difference between Chinese medicine group and western medicine group. After treatment, the score of the clinical symptoms, UA, ESR, CRP and IL-6 levels in integrated Chinese and western medicine group were better than those in TCM group and the western medicine group (P<0.05), but there was no significant difference between TCM group and the western medicine group. The incidence of adverse reactions was as follows, TCM group (3.6%) < integrated Chinese and western medicine group (60.5%) < western medicine group (82.1%) (P<0.05); and the recurrence rate was integrated Chinese and western medicine group (7.4%) < TCM group (20.5%) < western medicine group (26.1%) (P<0.05). Conclusion: There was no significant difference in efficacy between Canshitang treatment and colchicine in the treatment of gouty arthritis syndrome of accumulated dampness-heat, but the incidence of adverse reactions and the recurrent rate were lower than those of colchicine. Canshitang combined with colchicine had a synergistic effect for this disease, and the efficacy was superior to that of Canshitang treatment alone or colchicine alone, with lower reverse action incidence and recurrent rate.关键词：Canshitang;gouty arthritis;syndrome of accumulated dampness-heat;colchicine;interleukin-6;therapeutic effect
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy and safety of Shentong Zhuyutang combined with western medicine in the treatment of rheumatoid arthritis. Method: A total of 120 patients with rheumatoid arthritis were randomly divided into treatment group and control group, with 60 cases in each group. The basic therapy included methotrexate 10 mg/time, 1 time/week, Leflunomide, 20 mg/time, 1 time/d, for oral administration. In addition to the basic therapy, the control group was also given Tripterygium glycosides tablets, while the treatment group was also given Shentong Zhuyutang. After 12 weeks of continuous treatment, the differences in the clinical curative effect between two groups were analyzed. Morning stiffness time, tender joint count, swollen joint count, resting pain, 20 m walking time, health evaluation questionnaire, as well as laboratory indexes C-reactive protein (CRP), rheumatoid factor (RF), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) were observed. Result: The total effective rate of the treatment group was 91.7%, which was higher than 83.3% of control group, with statistically significant differences between two groups (P<0.05). Compared with control group, DAS28 score, morning stiffness, rest pain, tender joint count, swollen joint count, 20 m walking time and HAQ score of the treatment group were lower than those of the control group, but with handgrip strength higher than control group (P<0.05). CRP, ESR, RF, TNF-α and IL-1 in treatment group were significantly lower than those in the control group (P<0.05). Conclusion: Shentong Zhuyutang combined with western medicine showed stronger advantages in improving efficacy, controlling symptoms, improving laboratory indexes in the treatment of rheumatoid arthritis, with a higher safety, and is worth clinical promotion and application.关键词：Shentong Zhuyutang;combined administration;blood stasis syndrome;rheumatoid arthritis
- 6
- |
- 4
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Jiawei Erchentang polycystic change, endocrine and metabolism in patients with polycystic ovary syndrome (PCOS). Method: A total of 157 cases of PCOS patients treated in our hospital from March 2015 to March 2016 were selected in this study, and divided into two groups by the random number table, with 78 cases in clinical group and 79 in control group. Patients in control group were treated with cyproterone acetate tablets. In addition to the therapy of the control group, the clinical group was also given Jiawei Erchentang. Then efficacy, polycystic change, endocrine and metabolism were compared. Result: The total effective rate of control group was 81.01%, while that of clinical group was 94.87%, with a significant difference (P<0.05). Compared with before treatment, ovarian volume and ovarian follicle conditions of two groups after treatment decreased (P<0.05). Compared with control group, ovarian volume and ovarian follicle conditions of the experimental group were lower (P<0.05). Compared with before treatment, luteotropic hormone (LH), follicle-stimulating hormone (FSH) and testosterone (T) of two groups after treatment decreased, while estradiol (E2) increased (P<0.05). Compared with control group, LH, FSH and T of the experimental group were lower after treatment, while E2 was higher (P<0.05). Compared with before treatment, glucolipid metabolism indexes, including hemoglobin Alc (HbA1c), fasting insulin (FINS), insulin resistance index (HOMA-IR), total cholesterol (TC), triglycerid (TG) of two groups after treatment decreased (P<0.05). Compared with before treatment, visfatin (VF) and leptin (LEP) of two groups after treatment decreased, while adiponectin (APN) increased (P<0.05). Compared with control group, VF and LEP of the experimental group were lower after treatment, while APN was higher (P<0.05). Conclusion: Jiawei Erchentang has a better effect on PCOS, and can significantly improve polycystic change, endocrine and metabolism, thus is worth clinical promotion.关键词：Jiawei Erchentang;polycystic ovary syndrome (PCOS);polycystic change;endocrine;metabolism
- 4
- |
- 3
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of modified Zhenxin Dingxian Tang on the awareness function and the quality of life of patients with pediatric epilepsy (Tanhuo Raoshen symptom), anti-inflammatory and antioxidant stress. Method: One hundred and twenty-four patients were randomly divided into control group(62 cases) and observation group(62 cases) by random number table. Patients in control group were given levetiracetam, 10 mg·kg-1 for the initial treatment dose, which increased to 30 mg·kg-1, 2 times/day. In addition to the therapy in control group, patients in observation group were also given modified Zhenxin Dingxian Tang, 5 days/week. And 12 months were a course of treatment. Epileptic seizure frequency and duration were recorded. Before and after treatment, electroencephalogram was detected. And score of Tanhuo Raoshen symptom was graded. Quality of life questionnaire score (QOLIE-AD-48) and Wechsler Intelligence Scale for Children (WISC) were evaluated. And levels of high mobility group protein B1 (HMGB1), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD), and malondialdehyde (MDA) were detected. Result: The total effect rate in observation group was 92.86%, which was higher than 78.18% in control group (χ2=4.844, P<0.05). The total effect rate of electroencephalogram in observation group was 96.43%, which was higher than 81.82% in control group (χ2=6.143, P<0.05). After treatment, epileptic seizure frequency in observation group was less than that in control group, duration was shorter than that in control group, and score of Tanhuo Raoshen symptom was lower than that in control group (P<0.01). And scores of social support, epilepsy attitude, body function, school performance, health concept, shame, memory/attention and epilepsy of QOLIE-AD-48 scale and FIQ, VIQ and PIQ of WISC-CR scale were all higher than those in control group (P<0.01). And levels of HMGB1, IL-6, TNF-α and MDA were lower than those in control group, whereas level of SOD was higher than that in control group (P<0.01). Conclusion: Modified Zhenxin Dingxian Tang combined with levetiracetam can regulate epileptic seizure frequency, ameliorate the clinical effect, and improve the quality of life and the cognitive ability of patients, with certain anti-inflammatory and antioxidant effects and a better clinical effect than simple western medicine therapy.关键词：epilepsy;children;Zhenxin Dingxian Tang;function of cognition;inflammatory factor;oxidative stress
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of addition and subtraction treatment of Duhuo Jisheng Tang on ankylosing spondylitis (AS) with kidney deficiency, and investigate its effect on levels of bone metabolism and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and inflammatory mediators. Method: One hundred and twenty patients with AS were randomly divided into control group (60 cases) and observation group (60 cases) by random number table. Patients in control group got meloxicam tablets and sulfasalazine tablets, and the patients in observation group received addition and subtraction treatment of Duhuo Jisheng Tang. The treatment course was 4 months in both groups. Scores of visual analogue scale (VAS) for spinal pain, bath AS function index (BASFI), bath ankylosing spondylitis disease activity index (BASDAI), thoracic activity, lumbar vertebra activity (Schober test), pillow-wall distance, buttocks-ground distance, "4" figure test and physicians global assessment (PGA), as well as kidney deficiency scores were evaluated and recorded before and after treatment. Before and after treatment, levels of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), TNF-α, IL-6, human calcitonin (HCT), parathyroid hormone (PTH), bone alkaline phosphatase (BALP) and osteocalcin were detected, and safety was evaluated. Result: The compliance rate of assessment in ankylosingspondylitis 20 (ASAS20) was 70.91% in observation group, higher than 51.79% in control group (χ2=4.275, P<0.05). The compliance rate of ASAS50 was 54.54% in observation group, higher than 19.64% in control group (χ2=14.513, P<0.01). The compliance rate of ASAS70 was 32.72% in observation group, higher than 8.93% in control group (χ2=9.566, P<0.01). The compliance rate of BASDAI50 was 50.91% in observation group, higher than 16.07% in control group (χ2=15.156, P<0.01). Scores of VAS of pain, and BASFI, BASDAI, PGA, "4" figure test and physicians global assessment were lower than those in control group, and pillow-wall distance, finger-ground distance were shorter than those in control group, thoracic activity and lumbar activity were larger than those in control group after treatment (P<0.05, P<0.01). Score of kidney deficiency and levels of ESR, CRP, TNF-α, IL-6 in observation group were lower than those in control group (P<0.01). PTH levels were decreased in two groups (P<0.01), and there was no statistical significance between two groups. Level of BGP in observation group was higher than that in control group (P<0.01). Adverse reactions in observation group were less than those in control group. Conclusion: Addition and subtraction treatment of Duhuo Jisheng Tang can relieve symptoms, improve athletic ability, reduce disease activity, downgrade inflammatory factors and prevent loss of bone mass for ankylosing spondylitis.The clinical efficacy was better than that in western medicine treatment, with less side effects.关键词：ankylosing spondylitis;kidney deficiency;Duhuo Jisheng Tang;bone metabolism;inflammatory factor
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：On the basis of quality control of Gardeniae Fructus and its compound,pharmacokinetics of the representative chemical constituents in Gardeniae Fructus and its related compounds were analyzed,such as iridoid glycosides and diterpene pigments.This study summarizes the effects of the monomer administration,compatibility administration,compound administration on pharmacokinetics of active ingredients in Gardeniae Fructus.The results show that the quality control of Gardeniae Fructus and its compound is mainly aimed at the determination and fingerprint of single or multiple index components,but there is still a lack of research on quality control.Pharmacokinetic studies are concerned with the single components in Gardeniae Fructus,which can not reflect the pharmacokinetic characteristics of Gardeniae Fructus.Therefore,in order to provide a useful reference for the clinical use of Gardeniae Fructus,the author thinks that it is necessary to combine the quality control with Chinese medicine chemistry-pharmacological effects-pharmacokinetics and study its pharmacokinetics with multicomponent integration method.关键词：Gardeniae Fructus;traditional Chinese medicine compound;quality control;iridoid glycosides;pharmacokinetics;couplet medicines;geniposide
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Myocardial ischemia occurs when blood flow to heart is reduced or the myocardial oxygen demand is more than the maximum blood supply, resulting in changes in myocardial metabolism, function, and structure. Reperfusion after ischemia leads to cardiac functional damage, arrhythmias, and myocardial cell necrosis, known as ischemia/reperfusion injury. Its pathophysiology is complex, involving coronary artery stenosis, endothelial injury, platelet aggregation, energy metabolism disorder, oxidative stress, inflammation response, apoptosis, calcium overload and autophagy, forming a complex regulatory network. The current clinical medicines for myocardial ischemia and reperfusion mainly include antiplatelet drugs, beta blockers, calcium channel blockers, statins, RAS blockers, nitrates and thrombolytic therapy etc.Although there are many kinds of drugs mentioned above, the control effect is limited because of the single target of modern western medicine and many adverse reactions. With multiple targets and components, Salviae Miltiorrhizae Radix et Rhizoma, the main medicine for activating blood circulation and removing blood stasis, has been widely used in the treatment of cardiovascular disease, especially for the prevention and treatment of myocardial ischemic diseases. The water-soluble ingredient is acknowledged as the important active ingredient in treating cardiovascular diseases. The effect and mechanism of main water-soluble components (tanshinol, salvianolic acid A and salvianolic acid B) were compared in this paper to provide a theoretical basis for the clinical application of Salviae Miltiorrhizae Radix et Rhizoma.关键词：Salviae Miltiorrhizae Radix et Rhizoma;water-soluble ingredients;myocardial ischemia;reperfusion injury
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Depression is one kind of mental diseases and endangers human health seriously. Until now, Drug therapy has been the main treatment of depression. At present, most commonly-prescribed antidepressants are chemically synthesized, and most of which are monoamine neurotransmitters modulators. These drugs have many disadvantages, such as remarkable side effects, slow onset, expensive prices and poor treatment outcome. The traditional Chinese medicine(TCM) has a long history in treating depression, thus providing an alternative to antidepressants with better efficacy and less side effects.Chinese herbal compounds which have antidepressant effects, could be classified by mechanism as follows-relieving the depressed liver, calming the mind by nourishing the heart, regulating vital energy and dispelling phlegm, invigorating blood circulation to dissipate blood stasis. According to the source of TCM decoction pieces, single herbs and active ingredients can be divided into the following catagories-tonics, regulating Qi and promoting the circulation of blood, tranquilization and calming liver wind, clearing heat and removing dampness, relieving exterior syndrome and cough. The new research results and achievements of antidepressant pharmacological research will exert a profound impact on the idea of traditional syndrome differentiation and treatment as well as the habits in medicine employment. In this paper, anatomical structure and biochemical indicators are briefly discussed, and then TCM which have antidepressant effects, in recent years are reviewed. This review aims to provide reference to the research and development of herbal antidepressants.关键词：depression;antidepressant traditional Chinese medicine;pharmacology;experimental research
- 2
- |
- 3
- |
- 16
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0