最新刊期
卷 23 ， 期 23 ， 2017
- 摘要：Objective: To explore the processing mechanism of carbonized Gardeniae Fructus by investigating main chemical components change of Gardeniae Fructus before and after processing and its cooling blood for hemostasis on blood heat and hemorrhage syndrome rat model induced by dried yeast. Method: HPLC and UV were adopted to determine the content changes of main chemical components in Gardeniae Fructus before and after processing.Rat blood heating and bleeding model was established after oral administration of Gardeniae Fructus and carbonized Gardeniae Fructus to rats for 7 days.The changes of rat rectal temperature, plasma thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), plasma fibrinogen content(FIB), the change in whole blood viscosity and pathological changes in the lung were determined. Result: The contents of geniposide and crocins were dramatically dropped, while the content of total tannin was increased after the Gardeniae Fructus was fried to carbonized Gardeniae Fructus.Compared with model group, Gardeniae Fructus group could lower the body temperature of rats(P<0.01) and reduce the high, medium, and low shear viscosity of whole bolld (P<0.05, P<0.01), but it had no effect on coagulation indexes.Although carbonized Gardeniae Fructus could only lower body temperature of rats in the 0-4 h (P<0.01) and decrease the whole blood low shear viscosity (P<0.05), it could significantly shorten TT, APTT and PT(P<0.05, P<0.01) and lower the level of FIB (P<0.05).Histopathological observation indicated that carbonized Gardeniae Fructus could relieve the lung injury of blood heat and hemorrhage syndrome model rats. Conclusion: Carbonized Gardeniae Fructus has cooling blood for hemostasis and its processing mechanism may be associated with the content changes of geniposide, crocins and tannin.关键词：carbonized Gardeniae Fructus;geniposide;tannin;crocin;cooling blood for hemostasis;processing mechanism;whole blood viscosity
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To optimize formulation and preparation technology of montmorillonite-betaxolol hydrochloride liposomes (Mt-BH-LP), and evaluate its in vitro release performance and permeability. Method: Mt-BH-LP was prepared by ethanol injection combined with ammonium sulfate gradient method.With composite score of encapsulation efficiency and drug loading as index, Orthogonal test was used to optimize the formulation.The in vitro release of Mt-BH-LP was detected by dialysis method.With isolated corneas of rabbit as a model, the in vitro modified Franz diffusion cell method was used to measure the permeability of Mt-BH-LP.The permeability of Mt-BH-LP was further studied by taking human immortalized corneal epithelial cells (iHCEC) as model. Result: Optimum prescription was as following:ammonium sulfate concentration of 0.15 moL·L-1, lecithin quality of 225 mg, lecithin-BH (5:1), lecithin-cholesterol (9:1).Encapsulation efficiency of Mt-BH-LP was (75.85±2.15)%, its drug loading, average particle size and Zeta potential were (11.41±0.29)%, (218±22.32) nm and (17.03±0.25) mV, respectively.The cumulative release of Mt-BH-LP was 60.2% in 10 h, the corneal hydration value of Mt-BH-LP was (76.72±2.68)% within the normal range. Conclusion: Mt-BH-LP prepared by optimized formulation has high encapsulation efficiency and drug loading, which displays good sustained-release performance, and it has a good development of application prospect.关键词：betaxolol hydrochloride;montmorillonite;liposomes;human immortalized corneal epithelial cells;entrapment efficiency;drug loading;in vitro release;permeability
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study on the effect of several medicinal excipients, such as microcrystalline cellulose (MCC), soluble starch (ST), dextrin (DE), maltodextrin (MA) and sodium carboxymethyl starch (CMS-Na), on the moisture absorption and formability of Codonopsis Radix granules. Method: A single excipient or two kinds of excipients with different ratio were mixed with Codonopsis Radix extract by 1:1, the moisture absorption kinetics of Codonopsis Radix granules was fitted by Higuch equation, Weibull distribution and unitary quadratic curve equation model, respectively.The best prescription of this granules was evaluated by the moisture absorption, forming and dissolubility. Result: Optimum molding materials was mixture of MCC and DE, ratio of Codonopsis Radix extract-MCC-DE was 3:2:1, Codonopsis Radix granules could be totally dissolved in 2 min with forming rate of 85.41%, equilibrium moisture content of this granules within 48 h was 6.28%. Conclusion: The optimum process is stable and feasible, it can effectively reduce the hygroscopicity of Codonopsis Radix granules.关键词：medicinal excipients;Codonopsis Radix granules;extract;hygroscopicity;formability;equilibrium moisture content;microcrystalline cellulose
- 2
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: Parishin is one of the main bioactive ingredients in Gastrodiae Rhizoma with multiple beneficial properties, its structural formula is composed of gastrodin and citric acid, the degradation reaction of parishin is easy to occur in the cooking and heating process.This paper was in order to study on the degradation mechanism of parishin in processing of Gastrodiae Rhizoma by certain techniques and methods. Method: Based on the reaction kinetics, HPLC-DAD-ESI-MSn was employed to investigate the charaters of kinetics and mechanism of degradation of parishin in aqueous solution. Result: Temperature and pH were the main factors affecting the stability of parishin, and the hydrolysis of easter bond in the parishin was the primary degradation pathways, furthermore, a possible primary degradation pathway of parishin based on MS fragmentation characteristics was proposed. Conclusion: The increase in gastrodin content may be derived from the conversion of parishin, it is suggested that the processing conditions should be controlled during the production process of Gastrodiae Rhizoma.关键词：Gastrodiae Rhizoma;parishin;degradation pathway;gastrodin;degradation kinetics;temperature
- 2
- |
- 3
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: To apply electronic nose method distinguish Schisandrae Sphenantherae Fructus from different habitats, a new method for the determination of the origin of Schisandrae Sphenantherae Fructus. Method: To determine the best experimental conditions for the electronic nose technology of Schisandrae Sphenantherae Fructus by single factor test and orthogonal test, and distinguish the Schisandrae Sphenantherae Fructus samples from different producing areas based on principal component analysis and principal component analysis combined with linear discriminant analysis. Result: The results showed that the influencing factors of Schisandrae Sphenantherae Fructus were as follows from high to low:Storage time at room temperature > sample weight > air intake amount. The optimum parameters for the determination of Schisandrae Sphenantherae Fructus were 5.0 g sample weight, 100 mL·min-1 air intake and 10 min storage at room temperature. The experimental results showed that the discriminative power of Schisandrae Sphenantherae Fructus under the optimum conditions could reach 0.986.Principal component analysis could be used to distinguish the Schisandrae Sphenantherae Fructus samples from different areas, but this method showed no obvious distinction ability on Schisandrae Sphenantherae Fructus from Gansu Huating county, Jiangluo town and Wudu county. The method of principal component analysis combined with linear discriminant analysis was superior to principal component analysis for distinguishing the Schisandrae Sphenantherae Fructus samples from different producing areas, with a clear distinguishing result on the samples from Huating county, Jiangluo town and Wudu county in this experiment. Conclusion: In this study, electronic nose combined with the special smell of Schisandrae Sphenantherae Fructus was used for detailed distinction about Schisandrae Sphenantherae Fructus samples from different producing areas, with significant distinguishing effect. This study can provide a new idea and method for objective, simple and green identification of Schisandrae Sphenantherae Fructus samples from different producing areas.关键词：electronic nose;Schisandrae Sphenantherae Fructus;principal component analysis (PCA);linear discriminant analysis (LDA)
- 2
- |
- 3
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: Ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) technique combined with multivariate statistical analysis were employed to investigate the common and different components in Atractylodis Macrocephalae Rhizoma (AMR) from different regions, in order to provide scientific basis for the modernization and quality evaluation of traditional medicinal materials from the perspective of chemical composition. Method: ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) was adopted with mobile phase of 0.1% formic acid aqueous solution (A)-acetonitrile (B) for gradient elution, electrospray ion source (ESI) under positive ion mode was used to scan the data, the AMR samples from 7 areas were detected, component identification was carried out according to the accurate m/z of primary mass spectrometry and the characteristics of secondary mass spectrometry, combined with the relevant literatures and databases.Principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and other multivariate statistical techniques were used to cluster the different metabolic profiles and chemical compositions, and select difference components in AMR from different regions. Result: AMR from Zhejiang and other regions had obvious differences in compositions, there were 16 major differential compounds, such as tyrosine, methylation of atractylenolide Ⅰ, atractylenolide Ⅰ, Ⅱ and Ⅲ, atractylone and others. Conclusion: Invigorating spleen of AMR from Zhejiang is better than samples from other regions with relative low dryness, tyrosine and dehydroaromadendrene can be used as the index ingredients for genuineness evaluation of AMR.关键词：Atractylodis Macrocephalae Rhizoma;regions;atractylenolides;multivariate statistical analysis;atractylone;tyrosine;dehydroaromadendrene
- 2
- |
- 3
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To provide quality basis for the Panax notoginseng powder processed from different parts. Method: Sensory, colorimetric method, microscopic, physical and chemical, and content determination methods were used in this study. Result: The P. notoginseng powder processed from different medicinal parts showed significant difference in appearance color, microscopic characteristics, ash content and content determination, sensory and microscopic characteristics can't be used to distinguish the P. notoginseng commodity specifications. Color can be used to distinguish the powder made of main root, notch and tendons, as the powder made of the main root showed the most shallow color, which was yellow and white, while the powder made of notch and tendons showed slightly deeper color, which was brownish yellow and light brown. The results were consistent with colorimeter observations based on SPSS analysis, which showed that cut the color was deepest in notch, followed by tendons, and the main root had the most shallow color. Microscopic features can be used to distinguish the powder made of notch and the main root, the tendons. The powder made of notch showed fibers, which were not detected in the main root and the tendons; clustered crystal was not detected in main root, but appeared in the notch; the ash content was all qualified in main root, but some were not qualified in notch and tendons; acid insoluble ash content was all qualified. The contents of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in different parts of P. notoginseng were significantly different. Conclusion: The color difference, microscopic characters, ash content, and ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 contents can provide quality basis for Panax notoginseng powder.
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint chromatogram of Pericampylus glaucus for the quality control of multi-batches of medicinal material, and provide scientific basis for the research on quality standards of P. glaucus. Method: The separation was developed on C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.2% phosphoric acid as the mobile phase for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 327 nm and column temperature was 30℃. The HPLC fingerprint chromatogram of P. glaucus was set up, and the quality of 11 batches of P. glaucus was evaluated by similarity assay (Similarity evaluation system for chromatographic fingerprint of TCM, Version 2012.1) as well as principal component analysis and cluster analysis. Result: Eleven common peaks were marked in 10 batches of samples by using multi-point correction and Mark peak matching, with the fingerprint of S4 sample as the reference fingerprint; and chlorogenic acid was identified as compared with the control product. The similarity of fingerprint was all higher than 0.9.Samples from different producing areas were classified into four groups based on principal component cluster analysis. Conclusion: This method is highly stable, simple and reproducible. With stable fingerprint, this study has important significance on further research of P. glaucus pharmacodynamic material basis, and also provides a reference for the quality control and evaluation of Guangxi Zhuang medicine P. glaucus.关键词：Pericampylus glaucus;fingerprint;cluster analysis
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the HPLC fingerprint and the dynamic changes of alkaloid substances from the long malt with different sprout length in the germination process, and optimize the suitable sprout length from the perspective of chemical composition. Method: WondaSil® C18 column (4.6 mm×250 mm, 5 μm) was used with methanol (A)-0.1 mol·L-1 potassium dihydrogen phosphate (B) as the mobile phase for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 270 nm and column temperature was 25℃.The fingerprints for 10 batches of malt medicinal materials were set up, and their similarity assay was carried out to evaluate their quality by Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004A edition), and HPLC fingerprints of malt in the germination process were compared in the same condition. The dynamic changes of alkaloid substances in malt germination process were studied by HPLC and acid colorimetry. Result: There were nineteen common peaks in the fingerprints of ten batches of samples, of which peak 7 was hordenine. Peaks 5, 7, 8, 10, 12, 15, 16, and 17 were newly added after the germination of barley. The peak area of peaks 1, 6, 9 and 13 was gradually increased and then decreased to reach the maximum at the sprout length of 0.75 cm; and the peak area of peak 3, 5, 8, 17, 18 and 19 was gradually increased and then maintained stable in malt germination process. The content of total alkaloid was the largest at malt sprout length of 0.75 cm. Conclusion: The establishment of HPLC fingerprints of malt can provide reference for the quality control of malt medicinal materials. The chemical compositions of malt have obvious changes in the germination process. The optimal sprout length shall be 0.75-1.00 cm with the content of alkaloid in malt as the index.关键词：malt;germination;length;HPLC fingerprint;alkaloids
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC-PDA multiwavelength detection method for the simultaneous determination of citric acid, 5-hydroxymethyl furfural (5-HMF) and neochlorogenic acid contents in Method: XBridgeTM C18 (4.6 mm×250 mm, 5 μm) column was used with (A) with 0.5% ammonium biphosphate solution (pH 3.0)-acetonitrile (B) as the mobile phase for gradient elution (0-10 min, 95%-80%A; 10-15 min, 80%-70%A; 15-16 min, 70%-95%A; 16-21 min 95%A). The flow rate was 0.8 mL·min-1, and the column temperature was 25℃. The detection wavelength was set at 210, 284 and 327 nm. Result: The good linear relationships was shown within 63.30-8 100.00 mg·L-1 (r=0.999 9) for citric acid, 0.50-32.00 mg·L-1 (r=0.999 9) for 5-hydroxymethyl furfural, and 0.19-12.00 mg·L-1 (r=0.999 8) for neochlorogenic acid. The recovery of each component was in the range of 97.21%-101.01% and the RSD was less than 2.0%. The three kinds of indicator components in the test solution showed good stability at least within 24 h. The average mass fraction of citric acid, 5-HMF and neochlorogenic acid in Mume Fructus Pulp from different origins was 318.71, 1.99, 2.01 mg·g-1 respectively, and the quality of Mume Fructus Pulp in Zhejiang and Sichuan was the best. Conclusion: This method is accurate and rapid, with low cost, so it can be used for the determination of the three components in Mume Fructus Pulp, providing a reference for quality control in the food and medicine development of ebony.关键词：Mume Fructus Pulp;citric acid;5-hydroxymethyl furfural;neochlorogenic acid;HPLC;content determination
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To establish the characteristic map method for simultaneous determination of four kinds of active ingredients including arenobufagin in toad skins from different areas, and provide the basis for its quality control and medicinal material identification. Method: A Phenomenex Gemini (4.6 mm×250 mm, 5 μm)column was used with the mobile phase consisting of acetonitrile(A)-water(B)(0-30 min, 10%-45%A;30-45 min, 45%-60%A);flow rate 1.0 mL·min-1;column oven temperature was 35℃;detection wavelength was 296 nm.The characteristic map method was used to determine the contents of four bufogenin constituents such as arenobufagin. Result: The content of arenobufagin in the toad skin from Sichuan province was highest (0.252 4%, 0.158 4%, 0.181 1%, 0.125 8% and 0.108 8% respectively for large flowered skin, middle black skin, super large skin, brown skin and small black skin), followed by the black skin from Jiangsu province (0.227 5%). The content of cinobufotalin was highest in large flowered skin from Sichuan province (0.214 3%), followed by black skin from Jiangsu province (0.147 8%). The contents of cinobufagin in the small black skin from Sichuan province (0.051 0%) and small flowered skin from Shandong province (0.136 1%) were higher than those from other areas and varieties. In general, the contents of four kinds of active ingredients in Sichuan province were higher, especially the large flowered skin from Sichuan Province. Conclusion: The characteristic map method was accurate and reliable in determining the contents of these four bufogenin constituents, which can be used for identification and quality control of told skins. In addition, the contents of the four bufogenin constituents in different areas and varieties were significantly different.关键词：toad skin;arenobufagin;cinobufotalin;bufalin;cinobufagin;characteristic map method
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To investigate the antioxidant activities of the different polarity extracts from the aerial parts and the root of Salvia deserta. Method: 95% alcohol extract of the aerial parts and the root of S. deserta were partitioned in petroleum ether, ethyl acetate and n-butanol orderly to obtain corresponding extracts and water layer. Different extracts were configured to the appropriate concentrations of solution. The antioxidant activities of the extracts were determined by 1, 1-diphenyl-2-pieryl-2-picrylhydrazyl (DPPH) and O2-·radical scavenging and Fe3+ reduction capacity experiments in vitro. The results were compared with those of positive control Vitamin C (Vc) then. Result: All of the extracts showed stronger free radical scavenging activity than that of Vitamin C, and the free radical scavenging activity of alcohol extract from the aerial parts (DPPH·IC50 0.039 g·L-1, O2-·IC50 0.130 g·L-1) was stronger than that of the root (DPPH·IC50 0.160 g·L-1, O2-·IC50 0.160g·L-1). The order of reduction power of Fe3+ was:different polarity extracts of the root > Vc> that of the aerial parts. Conclusion: All of the extracts showed antioxidant activity, suggesting that the aerial parts of S. deserta can replace the root for use. The study provided a scientific basis for the rational development of S. deserta.关键词：Salvia deserta;antioxidant activities;polarity fractions;ferric reducing antioxidant power
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: An UPLC-MS method was developed for the simultaneous determination of isoorientin, isorhamnetin-3-O-β-D-glucoside and scopolin in plasma of rats after oral administration of effective part of Filifolium siburicum and the pharmacokinetic parameters of three components were calculated as well. Method: Concentrations of the three analytes in plasma were separated on a ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.7 μm) with mobile phase of 0.1% formic acid aqueous solution-acetonitrile (containing 0.1% formic acid) for gradient elution.Mass spectrometric detection was carried out by multiple reaction monitoring (MRM) with electrospray ionization source under negative ion mode.Single dose method was adopted, rats were oral treated with 1.0 g·kg-1 of effective part of F. siburicum. Result: All calibration curves showed good linearity (R2>0.997) over a wide concentration range for all analytes.The mean extraction recoveries of analytes were in the range of 80.87%-93.75%, and the intra-day and inter-day RSD were <15%.The peak times (tmax) of isoorientin, isorhamnetin-3-O-β-D-glucoside and scopolin were (32.40±4.16), (30.00±4.80), (20.10±5.10) min;the areas under the curve (AUC0-t) of them were (37.80±3.94), (48.83±3.05), (32.40±2.64) μg·min·L-1;the peak concentrations (Cmax) of them were (31.80±5.46), (28.00±2.54), (20.33±1.17) μg·L-1;the elimination half-life (t1/2β) of them were (114.06±22.19), (126.12±19.38), (148.14±24.10) min, respectively. Conclusion: This UPLC-MS is proved to be precise and accurate, and it is suitable for the simultaneous determination of blood concentration of isoorientin and other two components in biological samples.Rates of these three components into the body and the absorption are fast, but the absorption is not ideal.关键词：Filifolium sibiricum;pharmacokinetics;isoorientin;isorhamnetin-3-O-β-D-glucoside;scopolin
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate the effect of Xuanbi Antong formula (XBF) on ventricular remodeling in myocardial infarction rats and its mechanism. Method: In this study, the acute myocardial infarction model was established by ligating the anterior descending coronary artery in Wistar rats. After administration with XBF (1.22 g·kg-1·d-1) for 4 weeks, the effect of XBF on apparent signs and cardiac indexes of the Wistar rats were observed, the left ventricular ejection fraction (LVEF) observed by Doppler ultrasonography, the changes in cardiac morphology were observed by pathological examination, the expressions of tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6) and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA), and the expressions of Caspase-3 and Caspase-9 were detected by immunohistochemistry and Western blot. Result: Compared with model group, the survival conditions of rats in all treatment groups were generally improved after XBF treatment, LVEF were significantly increased (P<0.05), both LV end-diastolic inner diameter (LVIDd) and LV end-systolic inner diameter (LVIDs) were significantly reduced (P<0.05). Pathological examination showed that the myocardium degeneration of the rats treated with XBF was decreased in a good order, the myofilaments were complete, the gaps were more uniform, and the interstitial collagen fibers were significantly reduced. The mitochondrial structure of cardiomyocytes was significantly improved, the ultrastructure was clear, and the arrangement of myofilaments was more regular. TNF-α and IL-6 were decreased (P<0.01), and IL-10 was increased (P<0.05) in rats treated with XBF. The expressions of Caspase-3 and Caspase-9 were descended in rats treated with XBF (P<0.05). Conclusion: XBF for 'clearing heat, promoting blood circulation and removing phlegm' can significantly improve the LVEF of rats after ventricular remodeling resulted in myocardial infarction. The mechanism may be that XBF can decrease and inhibit inflammatory factors for promoting ventricular remodeling, such as TNF-α and IL-6, increase inflammatory inhibitor IL-10, and inhibit the expressions of Caspase-3 and Caspase-9.关键词：Xuanbi Antong formula (XBF);myocardial infarction;ventricular remodeling;Caspase-3;caspase-9
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of Yinxing Mihuan oral solution on human umbilical vein endothelial cells(HUVECs)injured by hypoxia/reoxygenation (H/R) through p38 mitogen activated protein kinase (p38 MAPK) regulating endothelial nitric oxide synthase(eNOS)/nitric oxide (NO) signaling pathway. Method: Hypoxia ischemia/reperfusion injury model was established by hypoxia/hypoglycemia/reoxygenation on HUVECs. Cultured HUVECs were divided into control group, H/R group, Yinxing Mihuan oral solution high(75 mg·L-1), low dose group(36 mg·L-1), p38 MAPK inhibitor SB203580, Yinxing Mihuan oral solution high dose+SB203580, eNOS inhibitor L-nitro-arginine methylester(L-NAME), Yinxing Mihuan oral solution high dose +L-NAME. Cell viability and cell injury were measured by cell counting kit-8 (CCK-8) and enzyme labeling method. Protein expression of phosphorylating-p38 MAPK (p-p38 MAPK)/p38 MAPK and phosphorylating-eNOS (p-eNOS)/eNOS were detected by Western blot. The levels of tumor necrosis factor-α (TNF-α), soluble intercellular adhesion molecule-1(sICAM-1), cysteine aspartate protease-3(Caspase-3)were determined by enzyme-linked immunosorbent assay (ELISA). NO content was measured by nitrate reductase method. Result: Yinxing Mihuan oral solution high and low dose group could significantly improve cell viability and reduce lactate dehydrogenase (LDH) content(P<0.05, P<0.01), inhibit p-p38 MAPK expression on injured HUVECs, decrease TNF-α, sICAM-1 levels in cell culture supernatant(P<0.05, P<0.01). The inhibited p-eNOS expression was significantly increased after administration of Yinxing Mihuan oral solution high dose and (or) SB203580(P<0.05). Yinxing Mihuan oral solution high dose significantly increased the NO content of damaged HUVECs. After the addition of the eNOS inhibitor L-NAME, Yinxing Mihuan oral solution high dose could antagonize the effects of L-NAME on decreasing NO production and Caspase-3 elevation in injured HUVECs. Conclusion: Yinxing Mihuan oral solution can prevent HUVECs against H/R injury by resisting inflammatory, regulating the endothelial system and inhibiting apoptotic through p38 MAPK regulating eNOS/NO pathway.关键词：Yinxing Mihuan oral solution;hypoxia reoxygenation;p38 mitogen activated protein kinase(p38 MAPK);eNOS;nitric oxide(NO)
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-metastatic effect of Zuoguiwan and its mechanisms of action. Method: The bone marrow metastasis mode of nude mice was established by intra-femoral injection. The tumor-bearing mice were randomly divided into three groups:control group, low-dose Zuoguiwan (21 g·kg-1 per day) treatment group and high-dose Zuoguiwan (42 g·kg-1 per day) treatment group. After successive administration for 8 weeks, the expression of human ck19 gene was detected by nest polymerase chain reaction (PCR) assay to determine the metastatic foci of MDA-MB-231 cells in bone marrow. Oris migration assay was performed to determine the effect of Zuoguiwan-containing sera on the migration of MDA-MB-231 cells. Reconstituted basement membrane invasion assay was conducted to decide the effect of Zuoguiwan-containing sera on the invasion of MDA-MB-231 cells. F-actin polymerization was detected with Alexa Fluor® 488 phalloidin and fluorescence microscopy. The effect of Zuoguiwan-containing sera on parathyroid hormone-related protein (PTHrP) and C-X-C chemokine receptor type 4 (CXCR4) expressions in MDA-MB-231 was determined by Western blot assay. Result: Compared with control group, both low-dose Zuoguiwan group and high-dose Zuoguiwan group exhibited a significant suppression on the expression of ck19 gene in bone marrow of tumor-bearing mice (P<0.01). Data from Oris migratory assay showed that Zuoguiwan-containing sera inhibited transforming growth factor-beta (TGF-β)-induced migration in MDA-MB-231 cells significantly. It was also found that the invasive ability of MDA-MB-231 cells through reconstituted basement membrane was inhibited by Zuoguiwan-containing sera in reconstituted basement membrane invasion assay. The amount of invaded cells in Zuoguiwan-containing sera treatment groups was less than that in control group obviously (P<0.01). Data from fluorescence assay showed that Zuoguiwan-containing sera inhibited F-actin polymerization of breast cancer cells. It was also showed that Zuoguiwan-containing sera suppressed the expressions of PTHrP and CXCR4 in Western blot assay, significantly. Conclusion: Zuoguiwan has a significant anti-metastatic effect on breast cancer cells. Its mechanism of action is correlated with the suppression on motility of breast cancer cells, and the down-regulation of PTHrP and CXCR4 expressions.关键词：Zuoguiwan;osteolytic metastasis;breast cancer;parathyroid hormone-related protein
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Ephedrae Herba and its splitting fractions on the rat models of kidney-Yang deficiency and edema, explore the relationship between efficacy and drug properties, and discuss its mechanism. Method: The rat models of kidney-Yang deficiency were established with hydrocortisone combined with doxorubicin hydrochloride. Doxorubicin hydrochloride(4, 3.5 mg·kg-1) was injected into the tail vein at day 1 and day 8, at the same time, hydrocortisone (3.75 mg·kg-1·d-1) was injected intraperitoneally for 15 days. After modeling, the rats were divided into model group, Guifu Dihuang pill group (1.4 g·kg-1), Ephedrae Herba decoction low (1.17 g·kg-1), medium (2.34 g·kg-1) and high (4.68 g·kg-1) dose groups, ephedra alkaloid (0.020 g·kg-1), non-alkaloid (0.105 g·kg-1), alcoholic acid (0.132 g·kg-1), and volatile oil (0.933 3×10-3 mL·kg-1) groups, and a normal control group was set up additionally. Rats in each treatment group were given with the corresponding drugs, and those in the normal and model groups were given with the same volume of normal saline, with ig administration for continuous 28 days. At the end of the administration, 24 h urine was measured by rat metabolic cage method. 24 h urinary protein was detected by Coomassie brilliant blue G-250 dye combination method (CBB method). The levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), triiodothyronine (T3), thyroxine (T4), estradiol (E2) and testosterone (T) were measured by enzyme-linked immunosorbent assay (ELISA).Western blot was used to detect the contents of aquaporin 1 (AQP1) and aquaporin 2 (AQP2). Result: Ephedrae Herba decoction and its splitting fractions could significantly increase the levels of 24 h urine output, cAMP, T3, T4 and E2 in serum (P<0.05, P<0.01); Ephedrae Herba decoction and alkaloid components could significantly reduce rat 24 h urinary protein, cGMP, T, AQP1 and AQP2 expression levels (P<0.05, P<0.01). Conclusion: Ephedrae Herba has significant diuresis promoting and detumescence effects, and alkaloids are the best effective components. Its mechanism may be related to the reduction of renal AQP1 and AQP2 expression.关键词：Ephedrae Herba;alkaloid components;kidney-Yang deficiency and edema;aquaporin
- 2
- |
- 3
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shaoyaotang on colon tissues JAK2/STAT3 and SOCS3, in order to discuss the mechanism of Shaoyaotang by treating the rat model of syndrome type ulcerative colitis (UC). Method: A total of 120 Wistar rats, including 60 male rats and 60 female rats, were divided into 6 groups:blank group, model group, high-dose (24 g·kg-1), medium-dose (12 g·kg-1), low-dose (6 g·kg-1) Shaoyaotang groups, and western medicine group (sulfasalazine group, 1 g·kg-1). High-fat, high-sugar, spicy food plus immune complex, TBNS (2, 4, 6-trinitro-benzene-sulfonic acid) and ethanol were used to replicate the syndrome type UC rat model. Wistar rats in different groups were given different treatments for consecutively 21 days. In Shaoyaotang group, Wistar rats were given high, medium and low-dose Shaoyaotang by gavage. In western medicine group, they were administered with sulfasalazine powder. In blank group and model group, they were given isopyknic normal saline for intragastric administration. After collecting abdominal aorta serum and colon tissues, sue-eosin methyl (HE) staining was used to observe the pathological section; Real-time PCR method was used to detect the contents of genes. And Western blot method was used to detect the contents of protein. Result: The model group showed inflammatory reaction, mucosa damage and ulceration under the endoscope, which indicated the successful modeling. Compared with blank group, model group's JAK2 and STAT3 protein and gene expressions increased significantly (P<0.05), while SOCS3 decreased significantly (P<0.05). Compared with the model group, in Shaoyaotang treatment groups, JAK2 and STAT3 protein and gene expressions were significantly decreased, and high-dose Shaoyaotang group showed the most obvious effect (P<0.01); SOCS3 increased dramatically, and high-dose Shaoyaotang group showed the most obvious effect (P<0.05). Conclusion: Shaoyaotang can improve the pathological changes in colon mucosal tissues of rats with syndrome type UC and reduce inflammation, which may be related to the inhibition of JAK/STAT3 signaling pathway by SOCS3, the generation of negative feedback regulation and the decrease in activation.关键词：Shaoyaotang;ulcerative colitis;syndrome;JAK/STAT3 signaling pathway;cytokine signaling inhibitor SOCS3
- 3
- |
- 3
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Fushen granules on the peritoneal ultrafiltration function, glyoxalase-1 (Glo-1) and advanced glycation end products (AGEs) in rats with peritoneal dialysis. Method: The chronic renal failure peritoneal dialysis rat model was induced through 5/6 nephrectomy. After successful modeling, the SD rats were randomly divided into 1.5% PD model group; 1.5% PD+Fushen granules intervention group; 4.25% PD model group; and 4.25% PD+Fushen granules intervention group (group E), with 17 rats in each group; the control group was also set up, with 15 rats. After 4 weeks, the rats were put to death, and their peritoneal tissues and serum were collected to calculate the peritoneal ultrafiltration volume; Western blot was used to detect the rat peritoneal organization Glo-1 protein expression. And enzyme-linked immunoassay (ELISA) was used to detect the content of serum AGEs. Result: Peritoneal ultrafiltration and serum AGEs content in Fushen granules intervention group were lower than dialysis model group of the same concentration, with statistically significant differences (P<0.05, P<0.01). Compared with the control group, Glo-1 protein content increased in the model group, with statistically significant differences (P<0.05). Glo-1 protein expression in group B was higher than that in group C, but with on statistically significant difference. Compared with group E, Glo-1 protein expression was significantly elevated, with statistically significant differences (P<0.05). Conclusion: Fushen granules can regulate the expression of Glo-1 and reduce the accumulation of serum AGEs, so as to protect the residual kidney function and delay peritoneal filtration and peritoneal fibrosis.关键词：Fushen granules;glyoxalase-1;peritoneal dialysis
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 -
Effect and Molecular Mechanisms of Xiaozhi Decoction on NASH Cell Model Induced by FFA in HepG2 Cell
摘要：Objective: To investigate the effect and mechanism of Xiaozhi decoction on free fatty acids (FFA)-induced non-alcoholic steatohepatitis (NASH) cell model. Method: The NASH model in HepG2 cells was induced by 0.5 μmol·L-1 FFA. The NASH cell models were established by different concentrations of Xiaozhi decoction and fenofibrate. Oil red O staining was used to observe the content of lipid droplets. Cell supernatant (nuclear factor-κ-gene binding) NF-κB p65, IkappaB kinase beta (IKKβ), Phospho-IκB kinase alpha/beta[Phospho-IKKα/β (Ser176/180)], Phospho-insulin receptor substrate[Phospho-IRS-1 (Ser307)] and insulin receptor substrate-1 (IRS-1) protein were detected by Western blot. Result: The contents of tumor necrosis factor-alpha (TNF-α) and interleukin-6(IL-6) in the supernatant fluid of the model group were significantly higher than those in control group (P<0.01). Meanwhile, the expression of IRS-1 was reduced (P<0.05), while the levels of IKKβ, p-IKKα/β (Ser176/180) and IRS-1(Ser307) phosphorylation and nuclear translocation of NF-κB p65 protein were increased in the model group (P<0.01). The expressions of IKKα/β (Ser176/180) and IRS-1 (Ser307) phosphorylation and nuclear translocation of NF-κB p65 protein in each dose group were decreased (P<0.01), especially in high-dose Xiaozhi decoction. The protein expression of IRS-1 was significantly increased (P<0.05) in control group. Conclusion: Xiaozhi decoction can improve lipid deposition and inflammatory reaction in NASH cell model induced by FFA. The mechanism may be related to the inhibition of intracellular IKKα/β (Ser176/180) and IRS-1 (Ser307) phosphorylation and IKKβ, NF-κB nuclear protein expression.关键词：non-alcoholic steatohepatitis (NASH);HepG2 cell model;Xiaozhi decoction;free fatty acids;nuclear factor-κ-gene binding (NF-κB);IkappaB kinase α/β (IKKα/β);insulin receptor substrate-1 (IRS-1)- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of curcumin on the apoptosis of adrenal pheochromocytoma (PC12) cells induced by β-amyloid protein 25-35 (Aβ25-35) and the expression of cytochrome C (Cyt C) in mtochondria of rats, in order to analyze the mitochondrial mechanisms of anti-apoptosis. Method: Aβ25-35 was used to treat PC12 cells, and the cellular model of Alzheimer's disease (AD) was established. The PC12 cells were treated with curcumin at concentrations of 0 (blank control group), 1.25, 2.5, 5, 10, 20, 40 μmol·L-1, respectively. The survival rate was measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide(MTT). The PC12 cells were randomly divided into the blank control group, the model group, the low-dose (10 μmol·L-1) curcumin group and the high-dose (20 μmol·L-1) curcumin group. The effect of curcumin on the apoptosis, the changes in mitochondrial membrane potential (Δψm), the distribution of Cyt C and the expression of Cyt C in mitochondria were evaluated by Annexin V-FITC/PI flow cytometry, JC-1 staining assay, immunofluorescence analysis and Western blot analysis, respectively. Result: Compared with the blank control group, the survival rate of PC12 cells was decreased significantly, and the apoptosis rate was increased in model group (P<0.01). Compared with the model group, curcumin significantly increased the cell viability and decreased the apoptosis rate (P<0.01). Compared with the blank control group, Δψm decreased significantly, the release of mitochondrial Cyt C to cytosol increase, and the expression of Cyt C in mitochondria down-regulated in model group (P<0.01). Compared with the model group, curcumin significantly increased Δψm, inhibited the release of mitochondrial Cyt C to cytosol and Up-regulated the expression of Cyt C in the mitochondria (P<0.05, P<0.01). Conclusion: Curcumin can inhibit the apoptosis of PC12 induced by Aβ25-35 by suppressing mitochondrial apoptosis pathway.关键词：Alzheimer's disease;curcumin;apoptosis;mitochondrial apoptosis pathway
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Arnebiae Radix oil ointment (AROO) on a rat model of pressure ulcer (PU) and its possible mechanism, in order to provide the experimental evidence for clinical application. Method: Rats were randomly divided into seven groups, namely blank control group, sham group, model group, positive control group, middle-dose AROO group, high-dose AROO group and iodine group. The PU model was induced by applying magnets over irons that were implanted under the skin of rats except for blank control and sham group. AROO was applied the next day after modeling. Rats in blank control, sham group and model group were treated with normal saline, where as the other groups were given the corresponding medicines once a day. After three days of administration, the morphologic and color changes in skin were observed, and the histopathological changes in pressed skin tissues were observed under microscope. Nuclear transcription factor-κB p65(NF-κB p65) was detected by immunohistochemistry; WST-1 and microplate method were used to detect the superoxide dismutase (SOD) and malondialdehyde (MDA) in rat serum. And the levels of interleukin-lα (IL-lα) and tumor necrosis factorα-α (TNF-α) in rat serum were detected as well by enzyme linked immunosorbent assay (ELISA). Result: Compared with blank control and sham group, compressed skin parts of the rats in model group were red and swollen; and pathological observation showed that the cell lines were disordered, the cell gaps were widened, and the inflammatory cells were infiltrated. The levels of TNF-α, IL-1α and MDA in the serum and the expression of NF-κB p65 were significantly increased, while the level of SOD was reduced. After being treated with AROO for 3 days, the swelling in compressed tissues subsided, the pathological status was significantly improved, the inflammation was relieved, and the content of SOD in middle and high-dose groups was significantly higher than that of model group. However, the content of MDA was significantly lower than that of model group (P <0.05, P<0.01), the levels of TNF-α and IL-1α in the serum of high-dose group were significantly lower than those in model group (P<0.05), and the NF-κB p65 protein expression in high-dose group was significantly lower than model group (P<0.05). Conclusion: AROO has a therapeutic effect on rat PU model. Its mechanism is possibly related to the regulation of NF-κB p65 expression and the content of IL-1α, TNF-α, SOD and MDA in rat serum.关键词：Arnebiae Radix oil ointment (AROO);rat model of pressure ulcer;inflammation;free radical
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of glycyrrhizin on light aging of human keratinocytes (HaCaT) cells induced by ultraviolet B (UVB) and relevant cytokines. Method: Different concentrations of glycyrrhizin were used to treat HaCaT cells. Methyl thiazolyl tetrazolium (MTT) was used to screen the effective and safe concentration of glycyrrhizin. The light aging model was set up through the irradiation of ultraviolet radiation B (UVB) at the irradiation dose of 30 mJ·cm-2, the activity of cells was determined by MTT method. The test kits were tested to determine the effect of glycyrrhizin on glutathione peroxidase (GSH-Px), catalase (CAT) and lactate dehydrogenase (LDH) in each group of cells. Western blot method was used to detect the effect of glycyrrhizin on interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) protein expressions. Result: The optimum effective concentrations of glycyrrhizin were 1×10-7, 1×10-6, 1×10-5 mol·L-1. Compared with the blank group, the cell proliferation rate of the UVB group significantly decreased (P<0.01); the activities of GSH-Px and CAT were significantly decreased, the activity of LDH was significantly increased (P<0.01); the expressions of IL-1β, IL-6 and TNF-α were increased (P<0.05, P<0.01). Compared with the UVB group, the cell proliferation rate of 1×10-7, 1×10-6, 1×10-5 mol·L-1 glycyrrhizin+UVB group was significantly increased (P<0.01); the activities of GSH-Px and CAT were significantly increased, the activity of LDH was significantly decreased (P<0.01); the expressions of IL-1β, IL-6 and TNF-α were decreased (P<0.05, P<0.01). Conclusion: Glycyrrhizin can improve the activities of GSH-Px and CAT, reduce the activity of LDH, decrease the secretion of cytokines, and thus inhibit light aging of HaCaT cells induced by UVB.关键词：glycyrrhizin;ultraviolet B;light aging;keratinocyte;cytokine
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Gualou Qumai Tang (GLQM) on serum lipids, adipokines and nephrin of podocyte in rats with diabetic nephropathy (DN) and discuss possible mechanism. Method: DN model was established by SD rats with high glucose and fat diet, unilateral nephrectomy and intraperitoneal injection of streptozotocin (STZ). After successful modeling, the rats were randomly divided into DN model group, low dose GLQM (GLQM-L), medium dose GLQM (GLQM-M), and high dose GLQM (GLQM-H) groups, valsartan group, and additionally a blank control group was set. The rats in GLQM low, medium and high dose groups received ig administration of 1.4, 2.8 and 5.6 g·kg-1 GLQM respectively from 4th week; the rats in valsartan group received ig administration of 4.8×10-3 g·kg-1, and the rats in control and model group were given with 2.8 g·kg-1 distilled water, once a day for 12 weeks. Then the general status of the rats was observed; serum levels of triglyceride (TG), cholesterol (CHO), high density lipoprotein (HDL) and low density lipoprotein (LDL) were detected by automatic biochemical analyzer; serum levels of adiponectin (APN) and leptin (LEP) were detected by using enzyme-linked immunosorbent assay (ELISA). Then pathological changes of renal tissues were observed under light microscope, morphological changes of podocyte under electron microscope and nephrin expression in renal tissues was detected by using Western blot method. Result: As compared with the model group, the TG, CHO and LDL levels were significantly decreased in low, medium and high dose GLQM groups (P<0.05, P<0.01), and HDL was increased significantly (P<0.05). The LEP level was significantly decreased in medium and high dose GLQM groups and valsartan group (P<0.01); APN was increased significantly (P<0.01). Pathological examination showed that the pathological changes of renal tissues and podocytes in the treatment groups were less than those in the model group. After the intervention of GLQM, the protein expression levels of nephrin were increased in Chinese medicine groups and valsartan group (P<0.05). Conclusion: GLQM can reduce renal tissue and podocyte damages and delay the development of DN by improving blood-lipid metabolism, interfering adipokine and maintaining the expressions of nephrin.关键词：Gualou Qumai Tang;diabetic nephropathy;podocyte;adiponectin;leptin;nephrin
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To analyze the effect of Gastrodiae Rhizoma polysaccharide (GRPS) on neutral transmitter in the immature rats with cerebral palsy. Method: A total of 58 SD immature rats were used in the experiment, and except in the blank control ones, common carotid arteries of all the left 48 rats were ligatured and combined with hypoxia treatment to establish cerebral palsy models in immature rats. The successfully modeled rats were randomly divided into model group, cerebrolysin group (2.5 mL·kg-1), GRPS low (150 mg·kg-1) and high dose (300 mg·kg-1) groups. After treatment for 21 days, Y maze and diving platform experiment were used to explore the memory ability of rats. The levels of nitric oxide (NO), acetylcholine esterase (ACHE), 5-hydroxytryptamin (5-HT), noradrenaline (NE) and γ-aminobutyric acid (GABA) in cerebral cortex and hippocampus were measured by using ELISA or chemical method. Real-time PCR and ELISA were used to detect the expression levels of endothelial nitric oxide synthase (eNOS) mRNA and protein in brain. HE staining was used to observe the hippocampus tissue structure. Result: As compared with normal blank group, the error number in behavior was significantly increased in model group rats; the step down latency was decreased; and also, the levels of 5-HT, NE and GABA in cerebral cortex and hippocampus of model group rats were significantly decreased, while the levels of ACHE and Glu were increased significantly (P<0.05, P<0.01). As compared with the model group, the levels of NO, NE, 5-HT, and eNOS in cerebral cortex and hippocampus of GRPS low and high dose group rats were increased, while the level of ACHE was decreased (P<0.05, P<0.01); the levels of NO, NE, and expression of eNOS in hippocampus of GRPS high dose group were increased, while the levels of ACHE were decreased (P<0.05, P<0.01). The regular cell position was observed in hippocampus of GRPS low and high dose groups, without edema. Conclusion: GRPS could improve the memory of cerebral palsy immature rats, and the mechanism may be associated with increasing NO, ACHE, NE, 5-HT and eNOS levels in cerebral cortex and hippocampus, and protection on hippocampus tissue structure of rats.关键词：Gastrodiae Rhizoma polysaccharide(GRPS);cerebral palsy;neutral transmitter;endothelial nitric oxide synthase (eNOS);cerebral cortex;hippocampus
- 4
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shengui Yixin decoction on plasma monocyte Toll-like receptors 4/nuclear factor kappa B (TLR4/NF-κB) signal pathway in patients with chronic heart failure. Method: A total of 124 patients with chronic heart failure were divided into control group (50 cases) and treatment group (74 cases) by random number table. The control group was given routine western medicine therapy. The treatment group was given Shengui Yixin decoction for 4 weeks, 1 agent/day in addition to the therapy of control group. Heart rate (HR), left ventricular ejection fraction (LVEF), left ventricular end diastolic diameter (LVDD), and 6 minute walking distance (6 mWT), TLR4, NF-κB, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin1β (IL-1β) mRNA and protein expressions in two groups before and after treatment were observed. Patients were followed up for 6 to 18 months after discharge. Rate of rehospitalization, fatality and the number of rehospitalization were compared between two groups. Result: Compared with control group, the total efficiency of treatment group increased significantly (P<0.05). After the treatment, HR, LVDD, TLR4, NF-κB, TNF-α, IL-6 and IL-1β mRNA and protein expressions of treatment group were significantly lower than those of control group; LVEF, 6 mWT of treatment group were significantly higher than those of control group (P<0.05). both groups showed alleviations compared with before hospitalization (P<0.05). Rehospitalization rate, death rate and frequency of rehospitalization of treatment group were lower than those of control group (P<0.05). Conclusion: Shengui Yixin decoction in treating patients with chronic heart failure can significantly inhibit TLR4/NF-κB signal pathway and inflammation and improve the patients' cardiac function.关键词：chronic heart failure;Shengui Yixin decoction;Toll-like receptor 4;nuclear factor kappa B
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Linggui Yangxin decoction on the cardiac function indexes of dilated cardiomyopathy (DCM) patients with the syndrome of heart Qi and Yang deficiency and anti-heart antibodies in DCM patients, in order to explore its possible mechanism. Method: The 142 DCM patients in Wuxi were randomly divided into 2 groups, traditional Chinese medicine(TCM) treatment group and control group. Among then, 40 patients with heart failure that were not caused by DCM were included into the CHF group, while 34 health cases were included into normal group. The control group was treated with standard therapy, without TCM, for 4 weeks, whereas the TCM treatment group was also given Linggui Yangxin decoction in addition to the therapy of the control group for 4 weeks. At the beginning and the end of the experiment, serum levels of Nterminalpro brain natriuretic peptide (NT-ProBNP), anti-β1 adrenergic receptor autoantibody, ant-β1(ant-β1), anti-myosin heavy chain autoantibody (ant-MHC), anti-muscarinic receptor-2 autoantibody (ant-M2), anti-adenine nucleotide (ADP/ATP) translocaseautoantibody (ant-ANT) were measured. Left ventricular dimension at end-diastole (LEVDd) and left ventricle ejection fraction (LVEF) were measured with echocardiography. To observe the clinical curative effect, the cardiac functional compensation of the DCM patients was observed in pre and post treatment. Result: The total effective rate of syndrome in the TCM treatment group was 89.6%, while that of the control group was 71.9%, indicating a significant difference (P<0.05). There was no significant difference between the DCM group and the control group in NYHA classification and grading of cardiac function (85.1% for treatment group vs 81.2% for control group). NT-ProBNP had significant differences before and after treatment (P<0.05). LVEF and LEVDd were significant improved in the two groups. Levels of ant-β1, ant-MHC, ant-M2, ant-ANT were significantly increased in the DCM group than in the CHF group (P<0.01). Levels of ant-β1, ant-MHC, ant-M2, ant-ANT were significantly increased in DCM group than normal group (P<0.01). After treatment, ant-β1 and ant-M2 were significantly different between the TCM treatment group and the control group (P<0.05). The incidence rates of hypotension, arrhythmia and stimulating dry cough in the TCM treatment group were lower than those in control group, with significant differences (P<0.05). Conclusion: Linggui Yangxin decoction can improve the expression of anti-myocardial antibodies, reduce the levels of NT-ProBNP, improve the clinical curative effect and the cardiac functional compensation, and reduce the incidence of adverse events. Its mechanism may be related to the improvement of anti-myocardial antibodies levels.关键词：Linggui Yangxin decoction;dilated cardiomyopathy;cardiac function;anti-myocardial antibody
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Duhuo Jisheng Tang in the treatment of liver-and-kidney-deficiency type knee osteoarthritis (KOA), and analyze the changes in small molecule compounds in synovial fluid before and after treatment with Duhuo Jisheng Tang. Method: A total of 73 patients with KOA diagnosed at the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine were selected and randomly divided into observation group (42 cases) and control group (31 cases). The observation group was treated with Duhuo Jisheng Tang, 150 mL/time, 2 times a day, for oral administration. The control group was given glucosamine sulfate capsules, 0.5 g/time, 3 times a day, for oral administration. Two groups were treated for a month. Visual analogue scale score (VAS), Western Ontario and McMaste Universities (WOMAC), self-made knee joint swelling score and comprehensive therapeutic response were taken as clinical outcome indexes. Synovial fluid of all of the patients with KOA was extracted before and after treatment. Supernatant liquid chromatograph and component analysis were used to analyze the differences in synovial fluid metabolism between two groups before and after treatment. PCA potential difference biomarkers were analyzed by EZinfo 2.0 software. Result: After 1 month of treatment, the scores of VAS, WOMAC and knee swelling of the patients in two groups were significantly alleviated compared with those before treatment (P<0.05). After 1 month of treatment, compared with control group, VAS, WOMAC, knee swelling score, comprehensive treatment response evaluation in observation group were significantly higher (P<0.05). Compared with before treatment, in observation group and control group, the levels of pantothenic acid in the knee joint fluid, the expression of tyrosine, tyrosine and the expression of benzoic acid, citric acid and proline were increased after treatment (P<0.05). Compared with control group, the expressions of pantothenic acid, kynurenine and tyrosine in the synovial fluid in observation group were lower, the expressions of benzoic acid, citric acid and proline were higher (P<0.05), and the expressions of taurine, β-cryptoxanthin and lysine in synovial fluid of observation group were significantly increased (P<0.05). Conclusion: Duhuo Jisheng Tang could reduce joint pain, swelling degree, and improve the range of activities and the quality of life of patients with liver-and-kidney-deficiency type KOA. At the same time, the expressions of pantothenic acid, kynurenine, tyrosine, benzoic acid, citric acid, proline, taurine, β-cryptoxanthin and lysine in synovial fluid were also changed with the improvement of symptoms in liver-and-kidney-deficiency type KOA patients.关键词：Duhuo Jisheng Tang;knee osteoarthritis;metabolomics;tricarboxylic acid cycle;taurine
- 3
- |
- 3
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: To observe the efficacy of Qiju Dihuangwan on dry eye in patients after cataract operation and its effect on scores of tear breakup time (BUT), schirmer I test (SIT), and fluorescent test (FL). Method: The subjects of this study were selected from 116 cases of dry eye in patients after cataract surgery treated at our hospital from February 2016 to January 2017, and divided into two groups by means of random number table, with 58 cases (64 eyes) in observation group and 58 cases (66 eyes) in control group. Both groups were given artificial tears, and observation group was additionally given Qiju Dihuangwan. The clinical effects and the changes in BUT, SIT, FL and dry eye symptom scores of two groups were compared. Result: The overall effective rate of the observation group was 93.75%, which was significantly higher than 77.27% of control group (P<0.01). Two 2 weeks, 1 month, 3 months later after operation, BUT in both groups was obviously prolonged (P<0.01), while the increase at each time point in observation group was more evident (P<0.05). Two 2 weeks, 1 month, 3 months later after operation, SIL in both groups was increased markedly (P<0.01), while the increase at each time point in observation group was more significant (P<0.05). Two 2 weeks, 1 month, 3 months later after operation, FL in two groups decreased (P<0.01), while the decrease was more significant in observation group (P<0.01). Two 2 weeks, 1 month, 3 months later after operation, the symptom scores also decreased remarkably (P<0.01), while the decrease in observation group was more significant (P<0.01). Conclusion: In the treatment of dry eye in patients after cataract surgery, Qiju Dihuangwan has a curative effect in improving the stability of tear film and the visual function.关键词：Qiju Dihuangwan;cataract operation;dry eye;efficacy;tear film
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study the clinical efficacy of Gegen Qinlian Tang for rectoclysis combined with massage manipulation on gastrin, motilin and somatostatin of children with damp-heat syndrome diarrhea. Method: A total of 100 cases of children with diarrhea treated in our hospital from August 2013 to October 2016 were selected and divided into two groups according to the random number table, with 50 cases in clinical group and 50 in control group. In addition to the routine western treatment, the control group was also given massage manipulation, while the clinical group was additionally given Gegen Qinlian Tang for rectoclysis. Then, treatment effect, fever, diarrhea, abdominal pain disappeared time, correct dehydration and resumption of feeding time, traditional Chinese medicine (TCM) syndrome score, and values of gastrin (GAS), motilin (MOT) and somatostatin (SS) were compared after treatment for 3 days. Result: The total effective rate of the clinical group was 94.00%, which was higher than 82.00% in control group, with significant differences (P<0.05). Fever, diarrhea, abdominal pain disappeared time, correct dehydration and resumption of feeding time in clinical group were obviously shorter than those in control group (P<0.05). Compared with before treatment, TCM syndrome score of two groups was significantly improved after treatment, with statistically significant differences (P<0.05). Compared with control group, TCM syndrome score of the clinical group was lower, with statistically significant differences (P<0.05). Compared with before treatment, GAS and MOT of two groups decreased, while SS increased after treatment (P<0.05). Compared with control group, the levels of GAS and MOT in clinical group were lower, while SS was higher after treatment (P<0.05). Conclusion: Gegen Qinlian Tang for rectoclysis combined with massage manipulation can significantly alleviate the symptoms of damp-heat syndrome diarrhea in children, shorten the treatment time, and improve gastrointestinal function by effectively coordinating the balance of gastrointestinal hormones.关键词：Gegen Qinlian Tang;massage manipulation;diarrhea;gastrin (GAS);motilin (MOT);somatostatin (SS);damp-heat syndrome
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy of Bixietang in treatment of rheumatoid arthritis (RA) cold-dampness syndrome and investigate its effects on levels of erythrocyte sedimentation rate (ESR), blood uric acid (UA), C-reactive protein (CRP) and superoxide dismutase (SOD). Method: A total of 114 eligible patients were randomly divided into control group and observation group, 57 cases in each group. Methotrexate tablets were given in both groups (10 mg/time, 1 time/week). Based on this, control group also received paeonia capsule (0.6 g/time, 2 times/d); and the observation group received Bixietang (1 dose/d). The treatment course was 3 months in both groups. The disease activity scores 28-joint counts (DAS28), the oswestry disability index (ODI), the health assessment questionnaire (HAQ) and the traditional Chinese medicine (TCM) differentiation RA cold-dampness syndrome (TCM symptom) scores were applied to estimate the efficacy. The changes in ESR, UA, CRP and SOD were detected in two groups, and the efficacy and incidence of adverse reactions were compared between two groups. Result: The 3 cases were withdrawn in control group, and 6 cases were withdrawn in observation group. The total effective rate was 94.1% in observation group, better than 79.6% in control group (P<0.05). After treatment, the improvement in scores of DAS28, ODI, HAQ, and TCM symptom was more obvious in observation group (P<0.05); improvement in ESR, UA, CRP and SOD was also more significant in observation group (P<0.05). The incidence of adverse events (gastrointestinal reactions, liver damage, oral ulcers, dermatitis, herpes simplex, and white blood cell decline) in observation group (7.8%) was lower than that (20.4%) in control group (P<0.05). Conclusion: Bixietang combined with western medicine in the treatment of RA cold-dampness syndrome was effectiveness to improve the clinical symptoms of RA, ESR, UA, CRP and SOD and decrease the adverse events.关键词：Bixietang;Methotrexate;rheumatoid arthritis;cold-dampness syndrome;therapeutic effect
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of oral Huoxue Shengji decoction and foot massage on early diabetic foot (DF) with Qi and Yin deficiency and syndrome of obstruction of choroid, and investigate its action mechanism from vascular endothelial function, oxidative stress and inflammation reaction. Method: One hundred and ten patients with DF were randomly divided into control group and observation group by random number table, 55 cases in each group. Patients in control group got mecobalamin injection at the place of protective measures, 0.5 mg/time, 3 times/week by intramuscular injection. Aspirin enteric-coated tablets, 100 mg/time, 1 time/day. Based on the treatment in control group, patients in observation group added oral Huoxue Shengji decoction and foot massage. The treatment course was 12 weeks in both groups. Peroneal nerve, motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV), and ankle brachial index (ABI) were detected. Scores of Qi and Yin deficiency, syndrome of obstruction of choroid and toronto clinical neuropathy (TCSS) were graded. And levels of nitric oxide (NO), visfatin and endothelin-1 (ET-1), malondialdehyde (MDA), superoxide dismutase (SOD), intercellular adhesion molecule-1 (ICAM-1), C-reactive protein (CRP) and interleukin 6 (IL-6) were detected. Result: The total effective rate was 84.% in observation group, higher than 66.67% in control group (χ2=4.072, P<0.05). Peroneal nerve, motor nerve conduction velocity (MNCV) and (SNCV) in observation group were all faster than those in control group (P<0.01). Dimensional scores of TCSS scale and the total score in observation group were lower than those in control group. After treatment, ABI level and ascending range in observation group were higher than those in control group (P<0.01). Scores of Qi and Yin deficiency and closed choroid obstruction in observation group were lower than those in control group, and degree of declining in observation group was greater than that in observation group (P<0.01). Levels of visfatin, ET-1, MDA, ICAM-1, CRP and IL-6 in observation group were lower than those in control group (P<0.01), and levels of NO and SOD were higher than those in control group (P<0.01). Conclusion: Oral Huoxue Shengji decoction and foot massage for early diabetic foot can improve peripheral vascular disease and neuropathy in patients, reduce clinical symptoms and signs, and the effect was better than the simple western medicine treatment. In addition, it can ameliorate the condition by regulating vascular endothelial function, reducing oxidative stress and inflammatory response.
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Shexiang Tongxin dropping pills combined with trimetazidine in treating ischemic heart failure(IHF) and its effects on inflammatory factors. Method: One hundred and twelve IHF cases were randomly divided into control group and research group with 56 cases of each group. The patients in control group were given with trimetazidine, tid and 1 tablet/time. Based on the treatment in control group, the patients in research group were also given with Shexiang Tongxin dropping pills, 2 pills/time and tid. The treatment course was 12 weeks in both groups. Left ventricular mass index (LVMI), mean wall stress (MWS), B-type natriuretic peptide (NT-Pro BNP), symptom scores of syndrome of deficiency of heart Qi and blood stasis, level of heart rate variability (HRV), and clinical efficacy were compared between two groups. The levels of S100 calcium binding protein A8/A9 complex (S100A8/A9), nuclear transcription factor (NF)-κB, and receptor for advanced glycation end products (RAGE) were detected in both groups. Result: After treatment, MWS, LVMI, NT-Pro BNP, and symptom score of syndrome of deficiency of heart Qi and blood stasis in research group were obviously lower than those in control group (P<0.01). The total effective rate was 94.83% in research group, superior to 79.31% in control group (P<0.05). Normal R-R interval standard deviation (SDNN), normal R-R interval mean standard deviation (SDANN), root mean square difference value of adjacent R-R intervals (RMSSD), adjacent normal R-R interval difference >50 ms beat number accounted for the total number of percentage of stroke (PNN50) were higher than the control group (P<0.01). And the levels of S100A8/A9, NF-κB, and RAGE in serum of research group were significantly lower than those of control group (P<0.01). Conclusion: Shexiang Tongxin dropping pills combined with trimetazidine had significant clinical efficacy in treating IHF, and can inhibit levels of inflammatory factor such as S100A8/A9, NF-κB, and RAGE.关键词：Shexiang Tongxin dropping pills;trimetazidine;ischemic heart failure;inflammatory factor
- 2
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：The historical evolution of the processing methods of Atractylodis Rhizoma are based on the theories, such as syndrome differentiation principles of treatment and three categories of etiological factors system, which can meet the need of traditional Chinese medicine(TCM) clinic requirement.There are different processing methods including rice-water-frying, bran-frying, coking-frying, etc.The raw decoction pieces of Atractylodis Rhizoma are effective at anti-inflammatory, antitumor and immune regulation.For which is considered as excellent medicine of dispelling wind, eliminating dampness and phlegm and flaccidity syndrome in TCM clinic.The bran-frying decoction pieces of Atractylodis Rhizoma, can moderate extremely dry property, strengthening spleen function.Which can also protect stomach and liver by promoting gastric emptying, regulating gastrointestinal motility, improving digestion and absorption function.And in this respect, they are considered better than the raw ones.The coking-frying decoction pieces of Atractylodis Rhizoma are always used as solid intestinal diarrhea in TCM clinic.In recent years, studies have shown that, the volatile components are the most important indicators for the evaluation of quality of Atractylodis Rhizoma, including atractylone, atrctylodin, acetylatractylodinol, etc.In this report, the different processing methods and the effective substance and evaluation of Atractylodis Rhizoma were summed up.The incidence relation among them were analysed for identifying the independent correlates and significant interactions, which can help us to elucidate the processing mechanism, establish quality control system and meet the TCM clinic requirement.关键词：Atractylodis Rhizoma;processing methods;quality evaluation;efficacy evaluation;volatile oil
- 4
- |
- 3
- |
- 9
发布时间：2024-01-04 - 摘要：As a kind of medicine and food homology plant with great utilization value, Lilii Bulbus is rich in resources and widely distributed in China. Phytochemical researches have shown that Lilii Bulbus contains, phenols-glycerides, alkaloids, flavones, amino acids, phospholipids, alkanes and other compositions, and mainly of steroidal saponins and polysaccharides. Pharmacological researches have shown that Lilii Bulbus exhibits excellent antitumor, antidepression, antioxidant, hypoglycemic, antifatigue, antihypoxia, immunomodulatory anti-inflammatory, and Na+/K+ ATPase inhibitory effect. Polysaccharides, steroidal saponins and alkaloids in Lilii Bulbus can inhibit the proliferation and promote the apoptosis of multiple cancer cells. Also, the steroidal saponin is the active ingredient for antidepression effect. The present paper reviewed the phytochemistry and pharmacological activities research in the past 30 years of Lilii Bulbus through accessing multiple databases such as CNKI, Wanfang, ScienceDirect, PubMed and Web of science, aiming to provide reference for further research and utilization of Lilii Bulbus.关键词：Lilii Bulbus;chemical constituents;pharmacological effects;steroidal saponin;polysaccharide;antitumor;antidepression;antioxidant
- 3
- |
- 3
- |
- 17
发布时间：2024-01-04 - 摘要：As an important unit in the manufacturing process of traditional Chinese medicine(TCM), extraction directly affects the subsequent process and the quality of medicines.The development and improvement of extraction techniques play an important role in developing high-quality TCM products and promoting the benign development of TCM industry.In the extraction process of TCM, the medicinal ingredients of herbs can be easily dissolved and efficiently extracted by adding extraction auxiliary agents, such as acids, alkalis, surfactants and enzymes, which can change the pH of solvent, or change the surface tension between the medicinal herbs and solvents, or destroy the structure of the plant cell wall.Extraction auxiliary agents has been widely applied in the extraction of flavonoids, alkaloids, polysaccharides, organic acids, saponins, anthraquinones, triterpene acids, proteins and other compounds in Chinese herbal medicines, it can significantly improve the extraction efficiency, but also has some defects.By consulting the literatures, this paper expounds the application, characteristics, advantages, disadvantages and scope of application of extraction auxiliary agents, analyzes the existing problems and puts forward some countermeasures to provide new ideas for the application of extraction auxiliary agents in the extraction process of TCM.关键词：traditional Chinese medicine;extraction technology;extraction auxiliary agent;surfactant;enzyme;compound;active ingredients
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：The development of new drug carriers plays an important role in the research of pharmaceutical preparations, with the rapid development of nanotechnology and in-depth understanding of drug carrier.The glycosylation of food additives as a drug carrier research has received extensive attention.In recent years, glycosylated stevioside, glycosylated hesperidin and glycosylated rutin have become research focus.Stevioside, hesperidin and rutin as food additive are widely used in the food industry, they have good biological activity and health function with poor taste and low solubility.Glycosylation modification can increase diversity of their structure and function, so that they can self-assemble in water to form core-shell nano-structure with different aggregation number.Transglycosylated food additives as new drug carriers can increase the solubility of the drug and enhance the bioavailability with good safety.In this paper, the properties and biological safety, biosynthesis method and transglycosylated food additives as well as their application in new drug carrier materials were reviewed so as to provide new method and new thinking for its application in the field of medicine.关键词：glycosylation modification;stevioside;hesperidin;rutin;drug carrier;food additives;bioavailability
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Fermentum Rubrum is a traditional Chinese fermented medicine and has been used for nearly 1 000 years in China. In recent years, its lipid-reducing effect has attracted attentions to its medicinal value and health care effect. In particular, its statins ingredients with the main lipid-reducing effect have become the focus of science research and development of Fermentum Rubrum industry. At present, only lactone-type lovastatin is recognized as an index component in the most of current domestic quality standards of Fermentum Rubrum. Lipid-reducing ingredients in Fermentum Rubrum are complex. Its acid-type lovastatin has the main lipid-reducing effect, and other statins also have a synergistic lipid-reducing effect. It is unreasonable to regard lactone-type lovastatin as the only index component, which leads to varying qualities and impacts quality monitoring, safety and effectiveness of clinical medication. More seriously, it is difficult to detect the adulteration of lovastatin in Fermentum Rubrum, because of the unreasonable quality standard. Based on the previous studies, the authors collected, analyzed and summarized the literatures for Fermentum Rubrum at home and abroad in recent years, and systemically reviewed the research advances in detection methods of lipid-reducing ingredients of Fermentum Rubrum. This paper puts forward the suggestion of adding the quality control index components, in the hope of providing a theoretical reference for the comprehensive quality evaluation and standard improvement of Fermentum Rubrum.关键词：Fermentum Rubrum;lipid-reducing ingredient;lactone-type lovastatin;acid-type lovastatin;statins ingredient;detection method
- 2
- |
- 3
- |
- 7
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0