最新刊期
卷 14 ， 期 10 ， 2008
- 摘要：Objective:To investigate the effects of principal,assistant,complement,guide compatibility of Banxiahoupo decoction on contents of honokiol and magnololin and to analysis the internal relationship of its antidepresant compositions.Method:The HPLC method was used to determine honokiol and magnolol contents in water extracts of Banxiahoupo decoction and its principal,assistant,complement,guide compatibility.Results:One of the Assistants-Indian Bread enhanced the contents of honokiol and magnolol in principal and assistant compatibility,while the effects of other Complement Fresh Ginger were less than that of Indian Bread.The guide-Perilla Leat produced no significant effects on honokiol and magnolol contents in prineipal and assistant compatibility.The contents of honokiol and magnolol would be significantly decreased when any of the complement and guide drugs was missing in principal,assistant,complement,guide compatibility of Banxiahoupo decoction.Conclusion:These findings suggested that there was an internal relationship between principal,assistant,complement,guide compatibility of Banxiahoupo decoction and its antidepressant composition changes.关键词：Banxiahoupo decoction;compatibility;honokiol;magnolol;HPLC
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发布时间：2024-01-04 - 摘要：Objective:A HPLC method for determination of andrographolide's content in andrographolide-resin complexes was studied.Method:Hypersil C18 column was used with methanol-water(55∶45) as the mobile and the detection wavelength was set at 225 nm.Results:linear in a range of 0.0956～0.956 μg the regression equation for andrographolide was Y=230.4X+47.264 with r=0.999 4.The average recovery was 99.99% and the RSD was 0.88%.Conclusion:The method is reliable and reproducible.It may be used for the determ ination of andrographolide in resin complexes of andrographolide ion-exchange resin.关键词：andrographolide;masking testing;resin complexes;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To develop an HPLC method for the determination of geniposide in Wujunzhidan Tablet(Fructus Mume,Fructus Gardeniac,Radix et Rhizoma Rhei,Fructus Citri Sarcodactylis).Method:Samples were separated on a YWG-C18 colum with the mobile phase of methanol-0.1%H3PO4(15:85)and the detection wavelength was set at 240 nm.Result:The calibration curve was linear in the range of(0.02～0.20)mg·mL-1(r=0.999 9)for geniposide.The average recoveriy was 95.8%,RSD=1.8%(n=6).Conclusion:The method is accurate and reproducible,and can be used for the quality control of Wujunzhidan Tablet.关键词：Wujunzhidan Tablet;geniposide;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC method for determination of geniposide and liquiritigenin in Legan-Qingyan Capsules.Methods: A Kromasil C18 Column(4.6 mm×250 mm,5 μm)was used.The mobile phase was methanol-0.5% glacial acetic acid,the flow rate was 1.0 mL·min-1,λ1=238 nm(for the geniposide),λ2=276 nm(for the liquiritigenin),the column temperature was 30 ℃.Results: The linear range for geniposide was within(54.1～162.4)μg·mL-1,r=0.999 8,recovery was 99.66%(n=9).The linear range for liquiritigenin was within(5.2～15.5)μg·mL-1,r=0.999 9,recovery was 99.90%(n=9).Conclusion:This method has good reproducibility.It can be used for quality control in production of Legan-Qingyan Capsules.关键词：HPLC;Legan-Qingyan Capsules;dual wavelength;geniposide;liquiritigenin;content determination
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发布时间：2024-01-04 - 摘要：Objective:To establish the method of the dissolubility of Andrographis paniculata dropping pills.Methods:Andrographolide as the indicator,the in vitro dissolution and HPLC method were applied to determine the accumulated dissolution rate of andrographolide at the different time.Results:The calibration curve was linear in the range of(4.932～394.56)μg·mL-1(r=0.999 9).The accumulated dissolution rate of Chuanxinlian dropping pills reached over 85% in 15 min.Conclusion:Chuanxinlian dropping pills which were prepared can improve the dissolution rate of poorly soluble drugs of the kind of andrographolide and advance the bioavailability.关键词：Chuanxinlian Dropping Pills;andrographolide;Invitro dissolution
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发布时间：2024-01-04 - 摘要：Objective:An HPLC method was established for the determination of Danshensu-Sodium.Method: Using Spherisorb C18(4.0 mm×250 mm,5 μm)with the mobile phase consisiting of acetonitrile-water-phosphoric acid(1∶30∶0.5);The detective wavelength was 280 nm;The column temperaure was ambient;The flow rate was 1.2 mL·min-1.Result:The standard curve was liner with the range of 25～250 μg·mL-1,r=0.999 2.The average recovery was 99.21%,RSD 0.98%.Conclusion: The method is simple and rapid which can be used for the quality control of Kuanxin Oral Liquor.关键词：Kuanxin Oral Liquor;HPLC;Danshensu-sodium
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发布时间：2024-01-04 - 摘要：Objective:To optimize the extraction procedure of Lixuekang Soft Capsule.Method:The procedure was configired by a sampling of orthogonal design with different ethanol extraction solvent uses.The yield of extracts and the total contents of emodin and chrysophanol were used as assessment index and based on different weighted coefficient for evaluation of the extraction efficiency of ethanol.Result:The optimal procedure was using the circulation reflux for 3 times with 8 times 70% ethanol,2 hours each time.Conclusion:The method can extract effectively active components in Lixuekang Soft Capsule and provide tab data for industrialized manufacture of Lixuekang Soft Capsule.关键词：Lixuekang Soft Capsule;extraction procedure;weighted coefficient;emodin;chrysophanol
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发布时间：2024-01-04 - 摘要：Objective:To optimize the extract technology of Danshen and Chishao in DanPu Keli with orthogonal design.Method:The different factors for the extracting rate of paeoniflorin and tanshinone ⅡA were researched with orthogonal design.Result:The refluence method is best one in all methods.The major influencing factors were the followings alcohol quantity,alcohol concentration,extraction time and extraction number.The best extracting technology was that adding eight times alcohol(80%),to reflux 1.5 hour and totally to extract 3 times.Three batchs tests showed that the extracting rate of paeoniflorin and tanshinone ⅡA were 91.2%±4.6% and 89.6%±5.2%,respectively.Conclusion:The optimum extracting condition was simple with a high efficiency.关键词：Danpu Keli;paeoniflorin;Tanshinone ⅡA;Orthogonal Design
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发布时间：2024-01-04 - 摘要：Objective:To examine the effects of Fuzhisan(FZS) on the AD model rats induced by injection β-amyloid(Aβ1-40) to the hippocampus.Methods:The AD model of rats was established by injection of Aβ1-40 to the hippocampus.The cognitive ability of the AD model rats was detected by the Morris water amaze test.The activities of acetylcholinesterase(AchE) and choline acetyltransferase(ChAT) in the hippocampus were examined by the colorimetric method;the acetylcholine(Ach) content was examined by the alk aline hydroxylamine colorimetric method.Results:The Chinese herbal midicine FZS not only effectively improved the learning and memory abilities of the AD model rats,in the water maze test,but also ameliorated the decline ChAT activity and Ach content in the hippocampus,and decrease the elevated AchE activity,The FZS was dose-dependently effective in the therapy of AD in rats ranging from 0.3 to 1.2 g·kg-1,however it allowed an optimal therapeutic effect at the dosage of 1.2 g·kg-1.Conclusion:The FZS compounds regulate and ameliorate the cholinergic functions of the AD-model rats,thereby achieve its therapeutic effect.关键词：FZS;AD;ChAT;AChE;Ach;cognitive ability
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of Wang Laoji Liangcha(WLJ) on plasma lipids metabolism and oxidative situation in restraint mice.Methods:The female mice were randomly divided into normal control group,stress control group,positive control groupe,two WLJ groups(125 mg·kg-1 and 500 mg·kg-1).After intravenously injected Intralipids into the 20 h restrained mice,the content of plasma lipid and lipoprotein lipase(LPL) activity were determined.The oxidative situation and antioxidtive capacity of plasma were measured.Results:Compared with the control groups,WLJ accelerated the basic metabolism of plasma lipids,improved the lipoprotein lipase(LPL) activity in restraint mice.And it also decreased the level of MDA and increased the antioxidant capacity of plasma(ORAC).Conclusion:WLJ improves the lipid metabolic dysfunction in restraint mice,and the mechanism might be related to the amelioration of oxidative situation in plasma.关键词：Wanglaoji Liangcha;restrain stress;lipid metabolism;ORAC;LPL
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发布时间：2024-01-04 - 摘要：Objective:To investigate in proteinuria and transforming growth factor β1(TGF-β1) in rat model of glomerulosclerosis and the intervention of Huoxue Bushen compound.Method:The rats model of glomerulosclerosis was developed by useing of unilateral nephrectomy plus doxorubicin injection.The experimental animals were randomly divided into normal control group,model group,western medicine control group,Chinese medicine prevention group and Chinese medicine treatment group.Dynamic observation of protein in urine during 24 h,and detection of plasma and kidney tissue of TGF-β1.Results:Proteinuria in glomerular sclerosis in rats showed a certain degree of change.With the progress of glomerulosclerosis,serum TGF-β1 increased tendenciously.Huoxue Bushen compound can reduce initial 24 h protein in urine of glomerulosclerosis.Conclusion:Compared with the normal control group,urine protein in the rat model group continued to increase.TGF-β increased in serum and kidney tissue.Huoxue Bushen compound can reduce glomerulosclerosis in the early proteinuria.关键词：TGF-β1;Glomerulosclerosis;Compound of Huoxue Bushen
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Danggui Buxue Decoction on function reconstruction of hematopoietic tissue in mice after bone marrow isotransplanttation(iso-BMT).Methods:The typical mouse model of iso-BMT was established.The model mice were randomly divided into BMT+Danggui Buxue Decoction treatment group(treated with Danggui Buxue Decoction by gastrogavage) and BMT model group(treated with normal saline by gastrogavage).PB and BM of normal mice were obtained as blank.The following parameters were observed in the day 1,11 and 22:peripheral blood cells,bone marrow mono-nuclear cells(BMMNC),and the observation of bone marrow under microscope.Results:The levels of peripheral WBC,RBC,platelet,BMMNC,and the observation of bone marrow by microscope at the day 11 and 22 in BMT+Danggui Buxue Decoction treatment group were all significantly better than that of BMT model group(P<0.05 or P<0.01).Conclusion:Danggui Buxue Decoction could improve the function reconstruction of hematopoietic tissue.关键词：Danggui Buxue decoction;bone marrow transplantation;hematopoietic tissue;function reconstruction
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of Naoli Zhibao(NLZB) on learning and memory acquired disorder induced by Scopolamine.Methods:Scopolamine was injected intraperitoneally to induce learning and memory acquired disorder in mice.The learning and memory capability of mice was investigated by step-down test,the activity of acetylcholinesterase(AChE) in serum and brain and the contents of free amino acids were measured.Results:NLZB significantly promoted the learning and memory capability of mice,and depressed the activity of AChE in serum and brain.On the other hand,NLZB could increase the contents of glutamate acid(Glu) and aspartic acid(Asp) in brain while the content of gamma-aminobutyric acid(GABA) was decreased.Conclusion:It is suggested that NLZB not only could regulate the center cholinergic system by depressing the activities of AchE and improving the level of acetylcholine in brain,but also could increase Glu/GABA ratio,which might be one of the mechanislms of NLZB to reinforcing intelligence.关键词：Naoli Zhibao;learning and memory;acetylcholinesterase;free amino acid
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Sanyangyixinkang Capsule on acute myocardial ischemia in dog.Methods:Acute myocardial infarction model in dog was established by ligating anterior descending coronary artery,using epicardial electrogram mapping to monitor the level of myocardial ischemia,using NBT histochemical stain to determine myocardial infarction size;using biochemical methods to determine AST,CPK,LDH;and using electron microscopy method to observe the morphologic changes of myocardial ischemia.Results:The drug can significantly reduce ∑-ST,N-ST,MIS,and serum AST and CPK levels.Conclusion:The drug has marked improvement and protection for acute myocardial ischemia in dog.关键词：Sanyangyixinkang Capsule;Dog;myocardial ischemia
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发布时间：2024-01-04 - 摘要：Objective:To explore further the pathogenesis of acquired aplastic anemia(AA) and the curative effect mechanism of Shengxue Mixture(SXM),witch is a Chinese materia medica compound with the function of reinforcing the spleen and nourishing the kidney and promoting blood circulation.Methods: Cyclosporin A as a control,mRNA and protein level and activity of NF-κB in bone marrow of mice model for immune-mediated aplastic anemia(AA) were detected with Q-PCR and immunohistochemistry technique before and after given SXM.Result: No significant changes for NF-κB mRNA expression were observed in bone marrow single nuclear cells(SNCs) of mice with AA,but NF-κB/p65 protein were highly expressed.Otherwise,p-IκBα protein lowerly.However,SXM could decrease NF-κB/p65 protein and increase p-IκBα.By comparison,not only NF-κB/p65 but also p-IκBαwas decreased significantly by Cyclosporin A.Conclusion: Abnormal protein expression and activity changes of NF-κB may play a role in mice model for infusion-induced AA.And SXM may improve hematopoiesis function of the mice by modulating expression and activity of NF-κB.关键词：acquire aplastic anemia;Shengxue Mixture;curative effect mechanism;NF-κB;immune-mediate
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发布时间：2024-01-04 - 摘要：Objective:To investigate the anti-inflammatory and analgesic actions of Zhiyanxiao Granule.Methods:The increased permeability of abdominal capillaries induced by acetic acid in mice,the inflammation induced by dimethyl benzene on mice ear and the granuloma formation induced by cotton pellet in rats were performed to observe the anti-inflammatory action of Zhiyanxiao Granule.Pain induced by acetic acid and hot-plate procedure in mice were performed to observe the analgesic action of Zhiyanxiao Granule.Results:Zhiyanxiao Granule could significantly decrease effusion of Evans blue from abdominal capillaries induced by acetic acid in mice,the swelling degree of mice ear induced by dimethyl benzene and the weight of granuloma induced by cotton pellet.It also retained response time and reduced the number of writhing in mice caused by acetic acid,as well as decreased the pain score of mice in hot-plate test.Conclusion: Zhiyanxiao Granule has the anti-inflammatory and analgesic actions,and may have good therapeutic effect on haemorrhoids.关键词：Zhiyanxiao Granule;anti-inflammation;analgesia
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发布时间：2024-01-04 - 摘要：Objective:To observe the anti-bacteria and anti-inflammatory effects of Qufeng Zhiyang(QFZY)Tincture.Methods: The in vitro anti-bacteria and the experimentation of skin infected by pathogenic bacteria were established to appraise the bacteriostatic effect of QFZY Tincture.The mice ear edema caused by xylene and rat's hind paw swelling inflammatory model caused by carrageenan were employed to study the anti-inflammatory effects.Results: QFZY Tincture had strong inhibiting effect on staphylococcus aureus,bacillus coli,pseudomonas aeruginosa,staphylococcus coat,bacillus distortion,candida albicans.It could alleviate symptom of skin infection elicited by staphylococcus aureus or pseudomonas aeruginosa.QFZY Tincture could significantly suppress ear swell induced by xylene in mice,hind paw tumefaction in rat and decrease PGE2 in inflammatory tissue.Conclusion:QFZY Tincture had preferably anti-bacteria and anti-inflammatory effects.关键词：Qufeng Zhiyang Tincture;anti-bacteria;anti-inflammatory
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发布时间：2024-01-04 - 摘要：Objective:To explore the method of isolation,cultivation and identification of rat vascular adventitial fibroblasts in vitro,and observe its biological behaviour.Methods:Dulbecco's modified Eagle's medium(DMEM) was the basic culture medium,being supplemented with 20% fetal bovine serum using for culturing thoracic aortal adventitia tissue pieces,and with 10% fetal bovine serum using for culturing passage cells.The fibroblasts were incubate at 37 ℃ in a humidified atmosphere of 5%CO2 and 95% air in the incubator.Growth kinetics,cellular morphologies were observed,and the cultured cells were identified by cell cytoskeleton immunostaining.Results: The isolated fibroblasts could grow and proliferate in vitro,and immunostaining of vimentin was positive,while which of desmin and vWF were negative.The exponential phase of growth was from the second to the fourth day after passage.Conclusion: Rat vascular adventitial fibroblasts can be cultured in stable condition in vitro,and sufficient and reliable target cells can be obtained for the study of mechanisms of vascular stenosis at molecular level.关键词：fibroblasts;cell culture;cell identification;biological behaviour
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发布时间：2024-01-04 - 摘要：Many diseases such as artherosclerosis,hypertension,ischemic heart disease can generate a lot of reactive oxygen species(ROS) which can cause oxidation stress.Recent studities showed that oxidation stress play an important part in the development of cardiovascular diseases,and the protection by Chinese herbs on those diseases was found,and may be considered as a new rationale of the prevention and treatment for the cardiovascular diseases.关键词：reactive oxygen species;oxidation stress;cardiovascular diseases
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发布时间：2024-01-04 - 摘要：Objective:To observe the clinical curative effect of icterus due to chronic cholestasis treated withpurgation dispersion purification harmonization.Methods: We analyzed etiology and pathogenesis of icterus due to chronic cholestasis and treating it under the guidance of Yin-yang,Zang-fu theory and molecular biological methods.We treated icterus due to chronic cholestasis with purgation dispersion purification harmonization,that is nourishing yin and fluid,circulating blood,Purging damp,heat,phlegm,and bloodstasis,and harmonizing Zang-fu.Results: The therapeutic method of purgation dispersion purification harmonization could erase patients' symptoms and signs,normalize their liver function indexes,including total bilirubin AST and ALT in serum.Conclusion: It was effective of Using purgation dispersion purification harmonization to treat icterus due to chronic cholestasis and its complications.It could help to clean up remnant icterus and lessen complications.关键词：cholestasis;integrated Chinese and western medicine;syndrome differentiation and treatment
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发布时间：2024-01-04 - 摘要：Objective:To review the progress in reducing the toxicity of Chinese medicines by using Chinese herb compatibility in the last ten years.Methods: Literatures on reducing the toxicity of Chinese medicines by using Chinese herb compatibility in recent years were summarized and analyzed.Results & conclusions: There are some advancements in studies on reducing the toxicity of Chinese medicines by using compatibility in recent years,but many researches are superficial.So it is necessary for us to elucidate the mechanism of detoxification by using compatibility by means of applying many modern multi-discipline methods.关键词：Chinese medicine;compatibility;detoxification;review
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