最新刊期
      卷 15 ， 期 9 ， 2009
      • DAI Wan-ying~1,SUN Wei-hong~2,MAO Shu-jie~1,GU Xue-zhu~1,NIU Zheng-rui~1,LI Xian-duan~
        Vol. 15, Issue 9, Pages: 1-4(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.031
        摘要：Objective:To develop the analytical method of five anthraquinones contained in rhubarb produced in Quanshui Village.To investigated the relativity between the contents of five constituents and the appearance.Method:The HPLC method is as follows:Kromasil-C18 column（4.6 mm×150 mm,particle diameter 5 μm）,detective wavelength is 254 nm,methanol-0.1% phosphonic acid solution（85∶15） as mobile phase.Results:Compared with the high-grade rhubarb,the content of five anthraquinones in inferior ones descented about 30%.Compared with the high-grade part,the content of five constituents in the inferior part of the same root tuber descented about 70～80%.The contents of five anthraquinones in pale yellow rhubarb were lower than that in yellow ones.Conclusion: The method with fine repeatability is suitable to evaluate the contents of anthraquinones in rhubarb and the results indicate the contents of five constituents are associated with the appearance.  
        关键词：Rhubarb Produced in Quanshui Village;Five anthraquinones;HPLC;quality evaluation   
        0
        |
        116
        |
        5
        <HTML>
        <引用本文> <批量引用> 30128203 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 4-5(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.032
        摘要：目的:建立软肝散结胶囊的质量控制方法;方法:采用高效液相色谱法测定处方中赤芍所含有的芍药苷含量;结果:芍药苷在2.412μg范围内呈良好的线性关系,r=0.999 9;平均回收率为98.7%,RSD为2.18%。结论:方法简便可靠,精密度高,分离度好,可作为软肝散结胶囊的质量控制方法。  
        关键词：软肝散结胶囊;芍药苷;高效液相   
        0
        |
        81
        |
        9
        <HTML>
        <引用本文> <批量引用> 30128191 false
        发布时间：2024-09-23
      • LIU Yong-gang~1,ZHAO Bao-sheng~1,WANG Xiu-li~1,HAN Shu-hai~
        Vol. 15, Issue 9, Pages: 6-7(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.033
        摘要：Objective:To establish a method for HPLC determination of icariin in Xianling gubao Dispersibie Tables.Methods: The condition for HPLC was C18 column（250 mm×4.6 mm,5 μm）,Agilent the detection wavelength was 270 nm.Results: The standard curve was liner in the range of 0.368～1.84 μg and the correlation coefficient was（0.999 9.）The coverage recovery was 99.84% with RSD 1.59%.Conclusion: The method shows good separating result,accuracy and reproducibility,can be used for the quality control of Xianling gubao Dispersibie Tables.  
        关键词：Xianling gubao Dispersibie Tables;icariin;HPLC   
        0
        |
        116
        |
        7
        <HTML>
        <引用本文> <批量引用> 30128454 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 8-9(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.034
        摘要：目的:测定白鲜皮中白鲜碱的含量。方法:样品加甲醇回流提取60 min,提取液蒸干,定容于25 mL容量瓶。采用HPLC法测定白鲜皮中白鲜碱的含量,使用C18色谱柱,乙腈-水（37∶63）为流动相,检测波长228 nm,流速1 mL.min-1,柱温40℃。结果:白鲜碱线性范围0.051.2μg,r=0.999 7,平均回收率为99.9%。结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可用于白鲜皮中白鲜碱的含量测定。  
        关键词：白鲜皮;白鲜碱;高效液相色谱   
        0
        |
        309
        |
        12
        <HTML>
        <引用本文> <批量引用> 30128419 false
        发布时间：2024-09-23
      • YUAN Qing-zhao~1,ZHANG Shui-han~2,YANG Ying~2,WU Lu~2,CAI Guang-xian~
        Vol. 15, Issue 9, Pages: 10-13(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.035
        摘要：Objective:To search for the micronization impact to the pill preparation,we carry out a series of comparative study on the micro characteristic、TLC characteristic、dissolution results of Ultro-micro and Normal powder of Zhizhu pill.Method:Using Fructus Aurantii Immaturus and Rhizoma Atractylodis Macrocephalae,the TLC information can be identified.Taking Hesperidin as the index component,the dissolution character were compared.The Weibull distribution analysis system were also applied.Result:We can see the almost similar TLC characteristic in the two pills,however the significant differences can be identified in the dissolution study on the Ultro-micro and the normal pills.Obviously,the dissolution rate and the total quantity of Hesperidin in the ultro-micro pills were increaseed.Conclusion:The active component of the pills can not be destructed in the process of micronization.Moreover,this behavior truly increase the vitro-utilization of the preparation.  
        关键词：Zhizhu-pills;advanced ultro-micro chinese prepared medicine;hesperidin;dissolution determination   
        0
        |
        229
        |
        11
        <HTML>
        <引用本文> <批量引用> 30128518 false
        发布时间：2024-09-23
      • FANG Tie-zheng~,FU Su-ping
        Vol. 15, Issue 9, Pages: 14-15(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.036
        摘要：Objective:An HPLC method was established for the determination of Rutin.Methods:Using Merck Lichrospher C18（5 μm,250 mm×4.6 mm） with the mobile phase consisiting of methanol-1% glacial acetic acid（32∶68）;The detective wavelength was 257 nm;The flow rate was 1 mL·min-1.Results:The standard curve was liner with the range of 0.27～7.11 μg（n=6,RSD=0.91%）,r=（0.999 5）. The average recovery was 99.54%,RSD 0.91%.Conclusion:The method is simple and rapid which can be used for the quality control of Zhiyanxiao Keli.  
        关键词：HPLC;Zhiyanxiao Keli;rutin   
        0
        |
        73
        |
        4
        <HTML>
        <引用本文> <批量引用> 30128547 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 16-17(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.037
        摘要：目的:对当飞利肝宁的原标准进行改进提高。方法:使用Waters2695-2487高效液相色谱仪,用CAPCELL PAK-C18柱（4.6 mm×150 mm,5μm）,以甲醇-乙腈-0.1%磷酸溶液（21∶14∶65）为流动相,检测波长为287 nm。结果:线性范围为0.160 28.008μg,R=0.999 98,平均回收率为98.24%,RSD=1.1%。结论:HPLC法简便、准确、重复性好,可用于测定当飞利肝宁胶囊中水飞蓟宾的含量测定。  
        关键词：高效液相色谱法;当飞利肝宁胶囊;水飞蓟宾;含量测定   
        0
        |
        108
        |
        5
        <HTML>
        <引用本文> <批量引用> 30128228 false
        发布时间：2024-09-23
      • HOU Feng-fei~1,ZHENG Ya-fu~2,ZHANG Hai-ming~2,SHEN Sheng-min~2,DU Shu-shan~
        Vol. 15, Issue 9, Pages: 18-20(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.014
        摘要：Objective:To investigate the chemical constituents in the Panzeria alaschanica Kupr.Methods:The constituents were isolated by column chromatography and their structures were determined on the basis of chemical and spectral data.Results:Seven compounds were isolated and identified as β-sitosterol（1）,stigmasterol（2）,5-hydroxyl-7,4′-dimethoxy flavone（3）,kaempferol-3-O-β-D-glucopyranoside（4）,β-sitostero-3-O-glucoside（5）,Rutin（6）,Quercetin（7）.Conclusions:All these Compounds except Compound（6） were isolated from Panzeria alaschanica Kupr.for the first time.  
        关键词：Panzeria alaschanica Kupr.;Compound;chemical constituent   
        0
        |
        397
        |
        13
        <HTML>
        <引用本文> <批量引用> 30128465 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 20-21(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.038
        摘要：目的:分析银翘解毒片中绿原酸的含量,为银翘解毒片的质量标准建立提供参考。方法:采用反相高效液相色谱法,Alltech C18色谱柱（4.6 mm×250 mm,5μm）;流动相为乙腈（A）-0.2%醋酸水（B）系统梯度洗脱;流速为1.0 mL.min-1;检测波长280 nm;柱温为室温。结果:绿原酸进样量在0.9812.25μg范围内线性关系良好（r=0.999 9）,测得平均回收率为99.8%,RSD为0.07%。结论:方法准确,结果稳定可靠,可用于银翘解毒片的质量评价。  
        关键词：银翘解毒片;绿原酸;高效液相色谱   
        0
        |
        93
        |
        5
        <HTML>
        <引用本文> <批量引用> 30128316 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 22-23(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.039
        摘要：目的:检测金银花中的绿原酸含量。方法:采用RP-HPLC的方法对购自全国各地的金银花药材进行绿原酸的含量测定。结果:18批不同购买地金银花样品中绿原酸的含量范围在0.8%7.2%,除少数几批达不到药典1.5%的标准外,其余大部分样品均合格。结论:购自青海和上海两地的金银花质量不符合规定。  
        关键词：银黄冲剂;金银花;绿原酸   
        0
        |
        269
        |
        15
        <HTML>
        <引用本文> <批量引用> 30128504 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 24-25(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.015
        摘要：目的:采用HPLC法测定参芍盆炎净合剂中芍药苷的含量;方法:采用Waters SunfireTM C18色谱柱,以乙腈-0.1%磷酸溶液（15∶85）为流动相,检测波长为230 nm;结果:芍药苷在0.0581.160μg之间线性关系良好,相关系数为0.999 9,平均回收率为98.8%,RSD为0.95%;结论:该方法稳定可靠,可用于参芍盆炎净合剂中芍药苷的含量测定。  
        关键词：参芍盆炎净合剂;芍药苷;高效液相色谱法;含量测定   
        0
        |
        41
        |
        2
        <HTML>
        <引用本文> <批量引用> 30128294 false
        发布时间：2024-09-23
      • XU Rong,CHEN Jun~,XU Hua,HAN Bang-xing
        Vol. 15, Issue 9, Pages: 26-29(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.016
        摘要：Objective:To optimize the extraction condition of total alkaloids from Rhizoma Coptidis and Cortex Phellodendri Chinensis.Methods:The extraction conditions of ratio of liquid to solid,extraction time and times were optimized by response surface method with the total alkaloids as the selective marker.The purification condition of total alkaloids was also studied.Results: The optimum extraction conditions were as follows: extraction repeated 4times,1 hour every time and ratio of liquid to solid was 18.125:1.The yield of total alkaloids under this condition was 66.44 mg·g-1 The total alkaloids were concentrated by acetic ether extraction under pH 12,with the ratio 1:1 of solvent to extract, also the extraction was repeated 6 times,then the content of total alkaloids was 66.130%.Conclusion:The experiment provides a basis for improvement of extraction condition of total alkaloids from Rhizoma Coptidis and Cortex Phellodendri Chinensis.  
        关键词：rhizoma coptidis;Cortex Phellodendri Chinensis;total alkaloids;RSM   
        0
        |
        818
        |
        28
        <HTML>
        <引用本文> <批量引用> 30128493 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 30-32(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.001
        摘要：目的:优选复方制首乌颗粒剂的水提工艺条件。方法:以浸膏得率和2,3,5,4′-四羟基-二苯乙烯-2-O--βD-葡萄糖苷含量为指标,采用单因素试验及正交设计法,确定复方制首乌颗粒剂的最佳提取工艺。结果:复方制首乌颗粒剂的最佳提取工艺为浸泡1h,提取3次,加水量依次为12,10,10倍,提取时间依次为2.5,2.0,2.0h,其中制首乌药材粉碎成粗粉。结论:本提取工艺简单、实用、具有可操作性,适合工业大生产。  
        关键词：二苯乙烯苷;提取工艺;正交设计;高效液相色谱法   
        0
        |
        200
        |
        2
        <HTML>
        <引用本文> <批量引用> 30128379 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 32-34(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.002
        摘要：目的:探讨活血龙胶囊的制备工艺条件。方法:通过高效液相色谱法对各过程中有效成分藜芦醇苷的含量进行检测,采用正交试验设计法优选活血龙有效成分的提取工艺,通过单因素考察,确定活血龙提取液的浓缩、干燥、制粒工艺。结果:制备过程中有效成分白藜芦醇苷保留率高;半成品颗粒饱满、流动性好。结论:该法适用于活血龙胶囊的制备。  
        关键词：活血龙;胶囊;制备   
        0
        |
        112
        |
        1
        <HTML>
        <引用本文> <批量引用> 30128406 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 35-37(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.003
        摘要：目的:研究益脑康宁滴丸的最佳成型工艺。方法:以丸重变异系数和外观作为评价指标,对冷却剂、滴速、滴距采用单因素考察,对药物与基质的比例、两种水溶性基质的比例及滴制液温度三因素进行正交试验。结果:优选的最佳成型工艺是甲基硅油与液体石蜡以2:1比例混合作为冷却液,药物与基质比例1∶1.5,基质聚乙二醇4000（PEG4000）与聚乙二醇6000（PEG6000）比例1.5∶1,滴制液温度70℃,滴速20 d.min-1,滴距8 cm,滴丸成型率最高。结论:以筛选出的成型工艺条件制成的滴丸,成品得率高,符合《中国药典》中滴丸剂的质量标准。  
        关键词：益脑康宁滴丸;正交试验;成型工艺   
        0
        |
        103
        |
        3
        <HTML>
        <引用本文> <批量引用> 30128218 false
        发布时间：2024-09-23
      • ZHOU Wei-qin~1,DENG Jing-yue~1,BI Ming-gang~
        Vol. 15, Issue 9, Pages: 38-41(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.017
        摘要：Objective:To probe the correlation between the effect of icariin on learning and memory and the level of estadiol（E2） in serum in senescence accelerated mouse prone/8（SAMP8）.Methods:10 six-mon-old senescence accelerated mouse resistance 1（SAMR1） and 40 the same age SAMP8 were selected.SAMP8 were divided randomly into 4 groups(model,75 and 150 mg·kg-1 icariin ig,and 0.2 mg·kg-1 diethylstilbestrol ig).Then,the effects of icariin administration by oral gavage for 3 months on learning and memory deficits in SAMP8 and SAMR1 were investigated by the Morris water maze test;the level of E2 in serum in SAMP8 and SAMR1 was determined by radioimmunoassay kit.The mice were sacrificed after ethology test,and the uterus coefficient was calculated. Results: The administration of icariin orally could enhance the learning and memory ability of SAMP8 in Morris water maze test;increase the level of E2 in serum in SAMP8;create non-significant difference in the uterus coefficient of SAMP8.There was a significant negative correlation between the latency in Morris water maze test and the level of E2 in serum in SAMP8 analyzed by Pearson correlation coefficient.Conclusions: Icariin could improve the cognitive impairments of SAMP8,and its mechanism may be related to the increase in the level of estrogen in serum in SAMP8.  
        关键词：icariin;senescence accelerated mouse prone/8;learning and memory;estradiol   
        0
        |
        386
        |
        27
        <HTML>
        <引用本文> <批量引用> 30128567 false
        发布时间：2024-09-23
      • MIAO Lan~1,LIU Jian-xun~
        Vol. 15, Issue 9, Pages: 42-46(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.040
        摘要：Objective:To explore the changes of rat serum proteins related with syndrome of Qi-deficiency and blood stasis and to clarify the molecular biomarkers of the syndrome.Methods: The rat models with Qi-deficiency and blood stasis were established by combined methods.The changes of serum protein expressing pattern were obeserved by two dimensional gel electrophoresis（2-DE）、Image Master 2D Elite 6.01 analysis software and MALDI-TOF-MS.Results:By MALDI-TOF-MS and then with the search engine MASCOT,compared with the normal group,6 proteins were identified,in which CD5 antigen-like was down-regulated,other 5 proteins including haptoglobin,complement component C3,etc.were up-regulated.Conclusion:The functions and features in these proteins had relevance to the pathological process of inflammation,immune regulation,which is consistent with the known occurrence and development of the syndrome of Qi-deficiency and blood stasis.  
        关键词：syndrome of Qi-deficiency and blood stasis;proteomics;serum;rat   
        0
        |
        636
        |
        37
        <HTML>
        <引用本文> <批量引用> 30128348 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 46-48(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.018
        摘要：目的:探讨黄芩、金银花、贯叶金丝桃、木蝴蝶、山楂70%乙醇提物的抗氧化作用。方法:低温条件下制备大鼠肝微粒体,采用Fe2+-ADP-NADPH体外氧化体系诱发肝微粒体脂质过氧化反应,通过计算半数抑制浓度评价上述5味中药的抗氧化作用。结果:黄芩、金银花、贯叶金丝桃、木蝴蝶、山楂醇提物的IC50分别为0.066,0.46,0.044,0.098,6.16 mg.mL-1。结论:黄芩等5种中药醇提物具有不同程度的抗肝微粒体脂质过氧化的作用。  
        关键词：黄芩;金银花;贯叶金丝桃;木蝴蝶;山楂;脂质过氧化   
        0
        |
        309
        |
        17
        <HTML>
        <引用本文> <批量引用> 30128531 false
        发布时间：2024-09-23
      • ZHANG Shou-lin~1,QI De-jie~2,SUN Wei-zheng~
        Vol. 15, Issue 9, Pages: 49-51(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.004
        摘要：Objective:To discuss the clinical effective of Busuishengxue granules to chronic aplastic anemia（CAA） patients,and to observe the difference in expression level of myelo-cytokine c-kit/CD117 before and after treatment.Methods:This experiment employed reverse transcription-polymerase chain reaction （RT-PCR） method to investigate the change of expression level of bone marrow mononuclear cell（BMMC） c-kit receptor mRNA and its c-kit receptor protein CD117 with flow cytometer method（FCM）.Results:The expression level of BMMC c-kit receptor mRNA and its receptor protein CD117 in CAA patients were higher than that in normal control group.And after treatment both of the expression levels descended to different degree.Moreover,the descent in Busuishengxue Granule group was superior to that in zaizhangshengxue Tablet group.Conclusions: There was abnormal expression of bone marrow SCF receptor c-kit/CD117 in CAA patients.Busuishengxue Granules by regulating the bone marrow c-kitmRNA and the expression of CD117 improved the hematopoietic function of bone marrow in CAA.  
        关键词：Busuishengxue Granules;aplastic anemia;Gene c-kit/CD117   
        0
        |
        103
        |
        1
        <HTML>
        <引用本文> <批量引用> 30128238 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 52-53(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.019
        摘要：目的:观察升白合剂对于环磷酰胺小鼠骨髓粒-巨噬细胞集落形成单位（CFU-GM）的影响。方法:环磷酰胺造模前3 d分别连续给予利血生及高、中、低剂量升白合剂进行干预,并于给药7 d后分别制备各组小鼠骨髓单个核细胞悬液,培养7 d后计数小鼠CFU-GM值。结果:各升白合剂干预组CFU-GM值明显高于模型组,其中高剂量组CFU-GM值明显高于利血生组,有统计学意义。结论:升白合剂有较好的提升环磷酰胺小鼠粒系祖细胞（CFU-GM）)的作用。  
        关键词：升白合剂;白细胞减少症;粒-巨噬细胞集落形成单位;环磷酰胺   
        0
        |
        85
        |
        5
        <HTML>
        <引用本文> <批量引用> 30128249 false
        发布时间：2024-09-23
      • LIU Chun-mei,TANG Yu-ping,GUO Jian-ming,WU De-kang,Ding An-wei~,DUAN Jin-ao
        Vol. 15, Issue 9, Pages: 54-58(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.020
        摘要：Objective: To investigate the chemical constituents and anticoagulant activity of Qin-Lian-Si-Wu decoction.Methods: Qin-Lian-Si-Wu decoction was extracted by hot-water,and then concentrated by decompression,and added ethanol till the concentration. of ethanol reached 80%.The upper solution was concentrated at（40 ℃,） dissolved by distilled water,separated by gradient elution with different concentration ethanol from macroporius adsorptive resins D101,then obtained the different fraction.Its constituents were isolated by silica gel column chromatographic method,and their structures were identified through UV,IR,NMR and MS spectrum.These compounds were prepared into aqueous solution,and then their effects on the blood plasma thrombin time in vitro in rabbits were determined.Result: Five flavonoids,one alkaloid,three aromatic acids,one sesquiterpene and two other components were isolated and identified from Qin-Lian-Si-Wu decoction.The results of thrombin time test showed that flavonoids,aromatic acids,alkaloid,sesquiterpenes could prolong the blood plasma thrombin time in vitro in rabbits remarkably.Conclusion: 12 compounds were isolated and identified from Qin-Lian-Si-Wu decoction for the first time.Nine compounds,including oroxylin A,wogonin,6-dimethoxy wogonin,berberine,baicalein,ferulic acid,vanillic acid,caffeic acid,rehmapicrogenin showed the anticoagulant activity at a certain dosage.  
        关键词：Qin-Lian-Si-Wu decoction;chemical constituents;anticoagulative activity   
        0
        |
        356
        |
        6
        <HTML>
        <引用本文> <批量引用> 30128480 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 58-60(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.005
        摘要：目的:研究扶正祛瘀中药抑制子宫肌瘤增殖的作用机制。方法:体外原代培养子宫肌瘤细胞并传代后,加入扶正祛瘀中药,继续培养,MTT法检测扶正祛瘀中药对子宫肌瘤细胞增殖的影响,免疫细胞化学法检测增殖细胞核抗原（PCNA）的表达。结果:扶正祛瘀组对子宫肌瘤细胞增殖有明显抑制作用（P<0.01）;扶正祛瘀组子宫肌瘤细胞PCNA的表达明显降低,与空白对照组比较有统计学意义（P<0.01）。结论:扶正祛瘀中药可能通过降低PCNA的表达,抑制子宫肌瘤细胞增殖,进而控制肌瘤的生长。  
        关键词：子宫肌瘤;细胞培养;扶正祛瘀中药;细胞增殖;增殖细胞核抗原   
        0
        |
        269
        |
        7
        <HTML>
        <引用本文> <批量引用> 30128327 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 61-63(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.021
        摘要：目的:研究益肾调肝方在非酒精性脂肪性肝炎大鼠的预防过程中对线粒体的调控作用。方法:用高脂饲料对SD大鼠造模10周,同时用益肾调肝方及易善复进行预防10周,然后检测各组大鼠肝组织线粒体指标,包括线粒体含量、细胞色素C氧化酶活性、线粒体膜电位、线粒体DNA修复酶等,以及肝功能、血脂及病理,并和正常组及模型组进行比较。结果:益肾调肝方使线粒体含量下降的幅度明显减小（P<0.01）;避免了细胞色素C氧化酶活性过度下降（P<0.01）;可使线粒体膜电位下降幅度减少（P<0.01）;使线粒体DNA修复酶水平明显升高（均P<0.01）;另外可以显著降低肝酶及血脂水平,减少脂肪沉积及改善肝脏病理。结论:益肾调肝方预防非酒精性脂肪性肝炎的过程中,对线粒体有明显影响,对线粒体的调控可能是益肾调肝方预防非酒精性脂肪性肝炎的作用靶点之一。  
        关键词：益肾调肝方;非酒精性脂肪性肝炎;线粒体含量;线粒体膜电位;细胞色素C氧化酶,线粒体DNA修复酶   
        0
        |
        154
        |
        12
        <HTML>
        <引用本文> <批量引用> 30128302 false
        发布时间：2024-09-23
      • ZHAO He-ping~
        Vol. 15, Issue 9, Pages: 64-66(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.041
        摘要：Objective:To observe the dynamic expression of L-FABP in rat nonalcoholic fatty liver disease（NAFLD） model and to discuss the role of L-FABP in the rat NAFLD.Methods:Fourty Wistar rats（200 g～220 g） were randomly divided into control group and model group.control group fed with standard diet;model group fed with high-fat diet（85% of the standard diet,13% of lard and 2% cholesterol）.The model group rat was put to death in 4,8,12 weeks seperately.Alanine aminotransferase in serum（ALT）,aspartate aminotransferase in serum（AST）,total cholesterol（TC）,triglyeride（TG）;mensurate fasting blood glucose（FBG）,Fasting insulin（FINS） were measured,and insulin sensitivity index（ISI） was calculated.HE staining was used to observe pathological changes in liver tissue;semi-quantitative reverse transcription polymerase Chain reaction（RTPCR） method was used to determine the expression of L-FABPmRNA changes.in rat liver tissue.Results:The expression of L-FABPmRNA was gradually increased in the rat liver of model group in 4,8 and 12 weeks（P<0.01）,the expression of L-FABP was gradually increased compared with the control group significantly（P<0.01）.In model group,the leral of serum ALT,AST,TC,TG was significantly higher than those in the control group（P<0.01）,while ISI decreased significantly（P<0.01）.Conclusion:Continuous 12 weeks high-fat diet can successfully replicate NAFLD rat model,and with extending the diet feeding time the model caused the expression of L-FABP increases gradually.  
        关键词：non-alcoholic fatty liver;liver-fatty acid binding proteiniL-FABP);reverse transcription polymerase chain reaction（RT-PCR）   
        0
        |
        158
        |
        14
        <HTML>
        <引用本文> <批量引用> 30128445 false
        发布时间：2024-09-23
      • XIE Li~1,XING Zhi-hua~1,JIANG RONG-xin~
        Vol. 15, Issue 9, Pages: 67-70(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.022
        摘要：Objective:To investigate the therapeutic effect of Sanpi Decotion in ulcerative colitis（UC） mice and discuss its therapeutic mechanism.Methods:Forty BALB/C mice were divided randomly and equally into normal group,model group,sulfasalazine（SASP） group,and Sanpi Decotion group（ten mice in each group）.After set up the oxazolone colitis,SASP group and Sanpi Decotion group were treated with sulfasalazine and sanpi decotion respectively,to observe the body weight,stool,food and drink situation of the mice every day.Killed all the mice on the tenth day,separated the spleen and weighed it,got the colon and evaluated the histological changes of the damaged colonic tissues,and measured the IL-2 and IL-10 concentrations in serum by using the enzyme-linked immunosorbent assay.Results: The symptom of Sanpi Decotion group and SASP group mice had great amelioration compared to model group mice,and compared to model group,the weight of spleen in Sanpi Decotion group and SASP group was increased,the concentration of IL-2 in Sanpi Decotion group was higher than that in model group（P<0.01）,and the concentration of IL-10 was lower than that in model group（P<0.01）.Conclusion:Sanpi Decotion is effective for treatment of UC and it can increase the concentration of IL-2 and decrease the IL-10 concentration,regulate the balance of the cytokines,improve the function of immunity,control the inflammation in the colon.  
          
        0
        |
        168
        |
        10
        <HTML>
        <引用本文> <批量引用> 30128262 false
        发布时间：2024-09-23
      • ZHONG Yi~,ZHAO Zhi-ming,LU Ying-jie,LIAN Zhi-cheng
        Vol. 15, Issue 9, Pages: 71-77(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.023
        摘要：Objective:To study the effect of "Weichangshu" and medical interfering on blood flow and gastro smooth muscle cells in rats of diabetic gastroparesis model.Methods: One hundred thirty-one male SD rats（SPF class） were randomly divided into the control group（10 rats）,model group（22 rats）,ethamsylate group（33 rats）,lumbrukinase group（33 rats） and WCS group（33 rats）.Rats were intraperitoneally injected streptozotocin（STZ） solution(60 mg·kg-1).After seven days,we established blood sugar more than or equal to 16.7 mmol·L-1 as experimental standard.Rats in the WCS group were treated by traditional Chinese medicine "WCS". So rats in the ethamsylate group treated by ethamsylate injection,rats in the lumbrukinase group treated by lumbrukinase enteric-coated capsules,rats in the control group and rats in the model group were treated by distilled water one time per day.After having been observed for 30 weeks,blood sugar,FINS,C-P and gastric fluid emptying rate were determined.Subsequently,samples of sinus ventriculi were made HE staining,Mallory staining and immunohistichemistry staining.Pathological lesion of gastric smooth muscle cells（SMC） was examined microscopically.Other sample of sinus ventriculi was made up electron microscopic specimen so as to know pathological lesion of ultrastructure of SMC through transmission electron microscope.Results: After 30 weeks,in the model group,endochylema of gastro smooth muscle cells were degenerated.The apoptotic index of SMC in rats of the model group was（24.33±2.34）% determined by TUNEL method,there was significantly difference compared with rats of the control group（P<0.01）.Protein expression of Bcl-2 in SMC was reduced in rats of the model group,but protein expression of Fas was overexpression,there was significantly difference compared with rats of the control group（P<0.01）.However,pathological lesion of gastro smooth muscle was obviously improved in rats of the WCS group and in rats of the lumbrukinase group.But in rats of the ethamsylate group pathological lesion of gastro smooth muscle was more serious.Conclusion:There were pathological Changes in gastric smooth muscle cells（SMC）in rats of diabetic gastroparesis model.Medicinal interfering in blood flow had the effect on gastric SMC pathology in rats of diabetic gastroparesis model."WCS" could decrease blood sugar and improve gastric SMC pathology and accelerate gastrointestinal motility.  
        关键词：Weichangshu;medicinal interfering in blood flow;rat;diabetic gastroparesis model;pathological changes in gastro smooth muscle cells   
        0
        |
        255
        |
        13
        <HTML>
        <引用本文> <批量引用> 30128363 false
        发布时间：2024-09-23
      • XU Zhi-fang~1,TANG Lu~1,CHEN Zhi-gang~
        Vol. 15, Issue 9, Pages: 77-80(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.024
        摘要：Objective:to discuss the effect of Panax notoginoside（PNS） on the neurologic impairment and（ ） 2008-06-18（ 陈志刚,）Tel:（010）67689776;E-mail:zgchen01@sina.com inflammatory factors IL-6,MMP-9 of rats with acute intracerebral hemorrhage.Method:The SD rats were divided into 3 groups randomly: sham group,model group and Panax notoginoside group.An animal model of ICH was edtablished by injecting collagenase Ⅶ into the caudate nucleus,and then Zea longa score,ICH mortality and the contents of IL-6 and MMP-9 in rat brain were measured.Results:Longa score of PNS group are lower than that of model group at the 5th day after ICH model establishment（P<0.05）.The improvement of neurologic impairment of PNS group at the 5th day compared with the 1st day are greater than that of model group（P<0.01）.The ICH mortality rate between PNS group and the model group had no significant difference.The contents of IL-6 and MMP-9 in brain tissues around hematoma of model group were significantly elevated at the 48th hour（P<0.01 and P<0.05）,and continued to rise till the 7th day（P<0.05 and P<0.01）.The inhibitive effect of PNS on the expressions of IL-6（P<0.05）,MMP-9（P<0.05）in brain on the 7th day was obvious.Conclusion:Panax notoginoside can promote recovery of neurologic impairment after ICH. One of the mechanisms may be that PNS could effectively inhibit IL-6 and MMP9.  
        关键词：acute intracerebral hemorrhage;IL-6;MMP-9;Panax notoginoside;neurologic impairment   
        0
        |
        258
        |
        18
        <HTML>
        <引用本文> <批量引用> 30128277 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 81-82(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.025
        摘要：目的:探讨参参康心滴丸对脑缺血再灌注损伤（CIRI）的影响。方法:制备大鼠CIRI模型,观察脑组织一氧化氮（NO）水平、内皮素（ET）、血栓素B2（TXB2）含量变化。结果:经过7天灌胃给药后,与模型组比较,参参康心滴丸高剂量组NO含量显著升高（P<0.01）,ET明显降低（均P<0.05）。结论:参参康心滴丸对大鼠脑缺血再灌注损伤具有良好的防护作用。  
        关键词：参参康心滴丸;缺血再灌注损伤;一氧化氮;内皮素;血栓素B2   
        0
        |
        142
        |
        7
        <HTML>
        <引用本文> <批量引用> 30128391 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 83-85(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.026
        摘要：目的:观察消肿止痛酊的主要药效学作用。方法:采用佐剂致大鼠关节炎、巴豆油致小鼠耳肿胀及棉球致大鼠肉芽组织增生、重物撞击致小鼠足跖部外伤性瘀血、冰醋酸致小鼠扭体等模型,观察消肿止痛酊局部皮肤给药的药效学作用。结果:消肿止痛酊局部用药能明显抑制佐剂致大鼠关节炎,有明显的抗炎作用及明显加速外伤性瘀斑的吸收,并有显著的镇痛作用。结论:消肿止痛酊具有抗炎、活血散瘀、镇痛作用。  
        关键词：消肿止痛酊;佐剂性关节炎;抗炎;活血散瘀;镇痛   
        0
        |
        274
        |
        6
        <HTML>
        <引用本文> <批量引用> 30128555 false
        发布时间：2024-09-23
      • ZHAO Ping~
        Vol. 15, Issue 9, Pages: 86-88(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.027
        摘要：Objective: To study the pharmacological effect of Zhenhuang Pills.Methods:The anti-bacteria,anti-inflammatory,antipyretic,analgetic,immunologic and the Huafu Shengji actions of Zhenhuang Pills were studied on the corresponding pharmacological models. Results: Zhenhuang Pills had strong inhibition on many kinds of inflammatory models and it showed analgetic effects and could obviously enhance the macrophage phagocytosis of mice and antisepsis effects.It also showed a certain degree of antipyretic effect in Escherichia coli endotoxin-induced temperature increment and accelerated the removal of the rotten tissue.Conclusion: Zhenhuang Pills have obvious anti-inflammatory,analgetic effects.it can increase physical immunity and has antisepsis effects.  
        关键词：Zhenhuang Pills;anti-bacteria;anti-inflammation;analgesia;immune   
        0
        |
        152
        |
        15
        <HTML>
        <引用本文> <批量引用> 30128431 false
        发布时间：2024-09-23
      • NIE Ya-xiong~,ZHU Yun-long,YIN Jian,LUO yan,LUO Wen-fang,XIE Ming
        Vol. 15, Issue 9, Pages: 89-92(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.028
        摘要：Objective:To observe the effects of the principle of restraining the hyperactivity of the liver yang on CD62p and ICAM-1 expressions in the cerebral vascular endothelium of renovascular hypertensive rats（RHR）.Methods:50 rats were divided into control group（n=10） and renovascular hypertensive rats group（n=40）.40 renovascular hypertensive rat were divided into four groups: model group,aspirin group,principle of restraining the hyperactivity of the liver yang group,principle of restraining the hyperactivity of the liver yang and aspirin group.The treatments Lasted 4 weeks after the establishment of the model.The rats were sacrificed and the tissues of the frontal lobe were slice into 5.0μm-thick frozen sections from the bregma section underwent in situ hybridization of ICAM-1 and CD62p mRNA expressions,the other five rats were examined by histochemistry under electron microscope and light microscope.Results:After treating,the plasma levels of CD62p and ICAM-1 in the principle of restraining the hyperactivity of the liver yang or aspirin group were obviously lower than that in the model group,the Aspirin group and the principle restraining the hyperactivity of the liver yang group.ICAM-1 and CD62p mRNA expressions and immunohistochemistry expressions in the principle of restraining the hyperactivity of the liveryang and aspirin group were obviously lower than that in the aspirin group and the principle restraining the hyperactivity of the liver yang group.Conclusions:Expressions of CD62p and ICAM-1 in plasma are significantly high in RHR at 12 weeks after operation.principle of restraining the hyperactivity of the liver yang obviously repress the expressions of CD62p and ICAM-1 in cerebral vascular endothelium of renovascular hypertensive rats,and the effect of treatment with principle of restraining the hyperactivity of the liver yang and aspirin in the RHR is obviously improved.  
        关键词：principle of restraining the hyperactivity of the liver yang;aspirin;platelet;CD62p;ICAM-1   
        0
        |
        135
        |
        1
        <HTML>
        <引用本文> <批量引用> 30128937 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 80(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.042
          
        0
        |
        51
        |
        0
        <HTML>
        <引用本文> <批量引用> 30128161 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 85-99(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.006
          
        0
        |
        97
        |
        0
        <HTML>
        <引用本文> <批量引用> 30128181 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 105-106(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.010
          
        0
        |
        94
        |
        5
        <HTML>
        <引用本文> <批量引用> 30128185 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 106(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.011
          
        0
        |
        71
        |
        0
        <HTML>
        <引用本文> <批量引用> 30128167 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 107-108(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.030
          
        0
        |
        215
        |
        31
        <HTML>
        <引用本文> <批量引用> 30128154 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 109(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.012
          
        0
        |
        142
        |
        0
        <HTML>
        <引用本文> <批量引用> 30128177 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 110(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.013
          
        0
        |
        74
        |
        12
        <HTML>
        <引用本文> <批量引用> 30128171 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 93-94(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.007
        摘要：目的:观察舒胃汤治疗肝胃不和型功能性消化不良的临床疗效。方法:随机选取门诊功能性消化不良患者60例,治疗组用舒胃汤,对照组用吗丁啉治疗,4周为1疗程。结果:治疗组总有效率为86.67%,而对照组仅为66.67%,治疗组明显优于对照组（P<0.05）;治疗组改善胃排空优于对照组。结论:舒胃汤可改善胃排空,治疗肝胃不和型功能性消化不良。  
        关键词：舒胃汤;功能性消化不良;胃排空   
        0
        |
        155
        |
        29
        <HTML>
        <引用本文> <批量引用> 30128795 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 95-96(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.008
        摘要：目的:观察天智颗粒治疗遗忘型轻度认知损害肝阳上亢证的疗效。方法:将96例病人随机分为天智颗粒治疗组、多奈哌齐片对照组,治疗前后观测认知功能、行为能力、证候积分。结果:两组认知功能改善无显著性差异（P>0.05）。治疗组日常生活能力改善及中医证候改善明显优于对照组（P<0.01）。结论:天智颗粒治疗轻度认知损害（aMCI）有较好疗效。  
        关键词：遗忘型轻度认知损害;肝阳上亢;天智颗粒   
        0
        |
        145
        |
        17
        <HTML>
        <引用本文> <批量引用> 30128830 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 97-99(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.009
        摘要：目的:观察风痛宁丸治疗类风湿性关节炎的临床疗效。方法:将150例患者分为2组,治疗组100例服用风痛宁丸,对照组50例服用雷公藤多苷片,2组均以2个月为1疗程,用药12疗程。结果:治疗组总有效率89.0%明显高于对照组72.9%,治疗组在改善临床症状、体征和实验室指标:血沉（ESR）、类风湿因子（RF）、C-反应蛋白（CRP）、免疫球蛋白（IgGI、gA、IgM）、补体3（C3）方面均优于对照组（P<0.01或P<0.05）,治疗组不良反应发生率显著低于对照组（P<0.01）。结论:风痛宁丸可综合改善类风湿性关节炎的关节及全身病变,无明显不良反应,并有较好的远期疗效。  
        关键词：风痛宁丸;类风湿性关节炎;临床疗效   
        0
        |
        107
        |
        7
        <HTML>
        <引用本文> <批量引用> 30128690 false
        发布时间：2024-09-23
      • XIA Xiao-hui~1,ZHANG Yu~1,XI Yan-bin~
        Vol. 15, Issue 9, Pages: 100-104(2009) DOI: 10.13422/j.cnki.syfjx.2009.09.029
        摘要：Flavonoids,terpene lactones and polyprenols are bioactivites actions of Ginkgo biloba L.Rencent progress on chemical constituents of Ginkgo biloba L were briefly reviewed,and structure of flavonoids,terpene lactones and polyprenols were detailed reviewed in this paper.  
        关键词：Ginkgo biloba L;chemical constituents;structure   
        0
        |
        2876
        |
        89
        <HTML>
        <引用本文> <批量引用> 30128870 false
        发布时间：2024-09-23
      • Vol. 15, Issue 9, Pages: 108(2009)
          
        0
        |
        63
        |
        0
        <HTML>
        <引用本文> <批量引用> 30128189 false
        发布时间：2024-09-23
      0