最新刊期
      卷 22 ， 期 18 ， 2016
      • WU Bing-bing,TANG Hui,ZHANG Bo,FENG Rong
        Vol. 22, Issue 18, Pages: 1-4(2016) DOI: 10.13422/j.cnki.syfjx.2016180001
        摘要：Objective: To investigate the in vitro stability of(-)-gossypol in different physiological pH and biological sample. Method: (-)-Gossypol was incubated in different phosphate buffer solutions with pH of 1.2-8.0,artificial gastrointestinal fluid and isolated biological media.The concentration of(-)-gossypol was determined by HPLC to investigate the degradation kinetics and the enzymatic stabilities of(-)-gossypol. Result: (-)-Gossypol was stable in pH 1.2 dilute hydrochloric acid solution,artificial gastric juice and ingastric contents,after 12 h of incubation,degradation rate of(-)-gossypol was less than 10%.(-)-Gossypol was unstable in phosphate buffer solutions at pH 6.8,7.4 and 8.0 after 12 h of incubation.The degradation rates of(-)-gossypol in small intestinal contents,large intestine contents and liver homogenates were 81.3%,96.9%and 97.3%after 8 h,degradation profiles of(-)-gossypol in gastric contents,small intestinal contents,large intestine contents and liver homogenates were similar to that in the aqueous buffer. Conclusion: (-)-Gossypol is stable in acid environment,but it is unstable in phosphate buffer solutions at different pH.(-)-Gossypol is stable in gastric contents,but it is unstable in liver homogenate,small intestine and large intestine bacteria.  
        关键词：(-)-gossypol;biological samples;stability;phosphate buffer;simulated gastric fluid   
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        发布时间：2024-01-04
      • QIAN Yan,SHAN Ming-qiu,ZHANG Li
        Vol. 22, Issue 18, Pages: 5-8(2016) DOI: 10.13422/j.cnki.syfjx.2016180005
        摘要：Objective: To elucidate the rationality of integration processing technology based on comparison on chemical constituents and pharmacological effect of Gastrodiae Rhizoma between traditional processing and integration processing. Method: Taking contents of gastrodin and parishins as indexes,chemical constituents difference in Gastrodiae Rhizoma between traditional processing and integration processing was compared,effects of Gastrodiae Rhizoma on eclampsia model induced by pentylenetetrazole were observed to compare products with different processing technology. Result: Contents of gastrodin,parishin A,parishin B,parishin C and parishin E in products of integration processing were 0.79%,2.04%,0.91%,0.23%and 0.57%,they were 0.39%,1.12%,0.64%,0.15%and 0.54%in products of traditional processing.The anti-eclampsia effect of Gastrodiae Rhizoma produced with integrated processing technology was better than traditional processing technology. Conclusion: Compared with the traditional processing technology,the integrated processing technology is better in guaranteeing the quality of pieces,reducing the cost of production and increasing the production efficiency.  
        关键词：Gastrodiae Rhizoma;integration processing;pentylenetetrazole;gastrodin;parishin A   
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        发布时间：2024-01-04
      • DAI Liang-min,XIONG Yong-ai,YANG Gui-yan,LIN Ying-ying,YANG Ming
        Vol. 22, Issue 18, Pages: 9-12(2016) DOI: 10.13422/j.cnki.syfjx.2016180009
        摘要：Objective: To optimize extraction and purification technology of total saponins in Sanguisorbae Radix by orthogonal test and three-step precipitation method. Method: HPLC-ELSD method was employed to determine the content of ziyuglycoside-Ⅰ with mobile phase of [methanol-acetonitrile(1:1)]-water for gradient elution.Taking transfer rate of ziyuglycoside-Ⅰ as index,orthogonal test was adopted to optimize extraction technology of total saponins.Three-step alkali precipitation method was used,single factor tests were adopted to optimize purification technology of total saponins with clearance of tannins and ziyuglycoside-Ⅰ content as indexes. Result: The best process was as follows:reflux extracted twice with 8 times the amount of 70% ethanol,each time for 1.5 h,adding 10%NaOH to adjust pH of extract between 12-14,stewing it for 12 hours,collecting supernatant solution after centrifugation,adding 10%NaOH to adjust pH of supernatant solution between 12-13,stewing it for 24 hours,collecting the precipitate after centrifugation,dried the precipitate at 70℃,then reflux extracted 30 min with 30 times the amount of ethanol,vacuum recovery of ethanol,dried 12 h.Purity of total saponins in Sanguisorbae Radix was 91.32%. Conclusion: The optimized extraction and purification technology of total saponins in Sanguisorbae Radix is simple and stable with high yield and purity,which is suitable for industrial production.  
        关键词：Sanguisorbae Radix;total saponins;alkali precipitation method;ziyuglycoside-Ⅰ;tannins   
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        发布时间：2024-01-04
      • CHEN Yin-fang,WANG Yan,SONG Xiao-ling,WANG Yue-sheng
        Vol. 22, Issue 18, Pages: 13-16(2016) DOI: 10.13422/j.cnki.syfjx.2016180013
        摘要：Objective: To establish the structural modified technology of gentiopicroside,and investigate the physicochemical properties of its acetylated product. Method: Orthogonal test was adopted to optimize the structural modified technology of gentiopicroside with reaction time,reaction temperature and molar ratio of acetic anhydride-gentiopicroside as factors,then single factor test was designed for optimizing the most significant factor.The apparent oil/water partition coefficient,stability test and powder properties were studied for investigating the physicochemical properties of the acetylated product. Result: Optimum acetylation process was established with pyridine as catalyst,acetic anhydride as acetylation agent,molar ratio of acetic anhydride-gentiopicroside(10:1),reaction at 20℃ for 12 hours.Yield of the acetylated product was 94.02%.The reaction product,purified by silica gel column chromatography,was determined to be tetra-acetylated gentiopicroside by ultraviolet spectrum and mass spectrum;lgP of the apparent oil/water partition coefficient(P) was 1.40,P reduced with increasing pH value.Tetra-acetyl gentiopicroside was sensitive to high temperature and acid,not sensitive to high humidity and strong light.Tetra-acetyl gentiopicroside had poor liquidity. Conclusion: The optimized technology for structural modification of gentiopicroside is stable and controllable.Absorption characteristics of the acetylated productin vivo is better than that of gentiopicroside,which can improve its bioavailability.  
        关键词：gentiopicroside;physicochemical properties;acetylation;structural modification   
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        发布时间：2024-01-04
      • LI Ming-xiao,ZHOU Zhen,TIAN Su-ying,LIN Jian-feng
        Vol. 22, Issue 18, Pages: 17-20(2016) DOI: 10.13422/j.cnki.syfjx.2016180017
        摘要：Objective: To optimize toxicity attenuation processing technology of Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma. Method: With the content of total alkaloids as index,toxicity attenuation processing technology of Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma was optimized by orthogonal test,and contents of alcohol-soluble extract and tussilagone in Farfarae Flos were determined before and after this optimized technology. Result: The best processing technology was as follows:adding 10% Glycyrrhizae Radix et Rhizoma,decocted three times,baking temperature at 90℃.Under these conditions,average content of total alkaloids was 0.080 3 mg·g-1,average content of tussilagone was 0.161%,average mass fraction of alcohol-soluble extract was 26.31%. Conclusion: After being processed,the content of total alkaloids in Farfarae Flos is decreased significantly,and contents of alcohol-soluble extract and tussilagone increase.This optimized processing technology is stable and feasible.This paper shows that Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma is rational.  
        关键词：Farfarae Flos;processed products with Glycyrrhizae Radix et Rhizoma;total alkaloids;tussilagone;toxicity attenuation technology   
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        发布时间：2024-01-04
      • DI Zhun,ZHAO Yan-li,ZHANG Ji,WANG Yuan-zhong,LI Li
        Vol. 22, Issue 18, Pages: 21-26(2016) DOI: 10.13422/j.cnki.syfjx.2016180021
        摘要：Objective: In this study, a high efficient and rapid method was used to identify the origin of herbal medicines in order to safeguard our country's economic interests in the international trade. Method: Ultraviolet(UV) spectroscopy combined with principal component analysis(PCA) and partial least square discriminant analysis(PLS-DA) was used to discriminate the Swertia davidi which collected from different origins and establish the prediction model to predict the accuracy of the regions. The spectra data were imported into UV Probe 2.34 software to compare the same part of S. davidi. Raw and pre-processed data(8 point smoothing, the first derivative and the second derivative) were imported into SIMCA-P 11.5 and the effect of discrimination of origins was compared by 3D score plot of PCA. Result: PCA indicated that the raw and 8 point smoothing data of leaves showed the best classification and the cumulative contribution rate of the first three factors was 98.8%. The other pre-processed methods could not obtain better identification and it may be related to the cumulative contribution value(the cumulative contribution rate of the data processed by the first derivative was 83.9%while the second derivative was 47.3%). Samples from Chongqing and Hubei could be distinguished with that of Hunan by the data of roots, but the samples of Chongqing and Hubei could not be separated. The model of PLS-DA may provide the basis of discrimination of more origins. The validation set was imported into the model developed by the training set and it proved that the model was feasible and effective. In PLS-DA, the correlation index of predictive value and true value in the training set was 0.985 and the RMSEE was 0.159. The correlation index of predictive value and true value after importing the validation set in the training set was 0.972 and RMSEP was 0.327. Both RMSEE and RMSEP were similar and less than 0.500. So the model had high reliability. Conclusion: UV spectra combined with PCA and PLS-DA can discriminate S. davidi from different origins and the predicted effect of the model was better. Furthermore, samples with unknown origins could also be distinguished.  
        关键词：principal component analysis;partial least square discriminant analysis;Swertia davidi;origin discrimination   
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        发布时间：2024-01-04
      • LI Xiao-lin,ZHAN Xiao-ri,LI Ying,WANG Rui-xia,SHAO Ai-juan
        Vol. 22, Issue 18, Pages: 27-30(2016) DOI: 10.13422/j.cnki.syfjx.2016180027
        摘要：Objective: To provide technical reference for standardization planting and long-term storage of Salvia miltiorrhiza seed by studying seed structure, germination and storage characteristics. Method: In the study of morphology and structure, seeds and their paraffin sections were observed, and photos were taken by the dissecting microscope and electron microscope. In the study of germination, the optimal temperature and different light conditions were investigated. In the study of storage characteristics, the changes in seed viability at different water content and temperature conditions were investigated to determine optimal storage temperature and water content. Result: ① S. miltiorrhiza seeds can well germinate at 15-35℃, with the optimal temperature between 20-30℃. Fluctuating temperatures at 15/25℃ and 20/30℃ can increase the germination rate. Light can decrease the germination rate at high temperature. ② Low-temperature storage was better than room-temperature storage. Higher or lower water content could lead to the sharp decrease of seed viability. The optimal storage condition was 4℃, with 5%of water content. ③ The pericarp of S. miltiorrhiza consisted of the mucilage cell layer, the parenchyma cell layer, the single sclereid layer and the sclerenchyma cell layer. After absorbing water, the mucilage cells swelled, ruptured and released a lot of gelatinous mucus. Conclusion: S. miltiorrhiza has a wide seed germination temperature range, and can be planted in most areas of China. In seedling, fluctuating temperatures can be used to promote seed germination and increase the seedling emergence rate of S. miltiorrhiza. Temperature and water content are important factors in the storage of S. miltiorrhiza seeds. The optimal storage method is low temperature and low water content. The characteristics of S. miltiorrhiza seeds of rapidly absorbing a large amount of water were related to the structure of the mucilage cell layer. This structure is conducive to maintaining the seed moisture and resisting the drought, but bad for seed storage.  
        关键词：Salvia miltiorrhiza seed;germination;storage;microstructure   
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        发布时间：2024-01-04
      • LI Min,CHEN Mei-jun,PAN Huan-huan,MEI Guo-rong,WANG Fu,CHEN Hong-ping,LIU You-ping
        Vol. 22, Issue 18, Pages: 31-34(2016) DOI: 10.13422/j.cnki.syfjx.2016180031
        摘要：Objective: To datalize the property and quality of Chinese medicine, identify the different species of Citri Reticulatae Pericarpium by its color information after using spectrocolorimeter, and provide a new method and idea for the study of common technical problems of traditional Chinese medicine. Method: Citri Reticulatae Pericarpium herbs were collected nationwide, and its color information was obtained by using spectrocolorimeter. According to the statistical analysis, a mathematical model for the identification of Citri Reticulatae Pericarpium species and 90%reference value scale were established. Result: A mathematical model for the identification of Citri Reticulatae Pericarpium species based on L*a*b* chromaticity space was established, with a coincidence rate of 100%; bilateral 90% reference range was as follows Citri Reticulatae Pericarpium: L*(66.621 5-73.916 5), b*(29.329 5-52.211 0); Citri Reticulatae Chachiensis Pericarpium: L*(53.950 0-63.260 0), b*(24.610 0-30.770 0). Conclusion: The color information from spectrocolorimeter can be used to accurately identify different species of Citri Reticulatae Pericarpium.  
        关键词：spectrocolorimeter;color;specie;Citri Reticulatae Pericarpium   
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        发布时间：2024-01-04
      • HAN Duo,ZHAO Zhi-lian,LIU Wei-hong,LI Hai-feng
        Vol. 22, Issue 18, Pages: 35-39(2016) DOI: 10.13422/j.cnki.syfjx.2016180035
        摘要：Objective: To investigate the differences between main secondary metabolites and molecules of Gentiana rigescens from different populations by the method of content determination and DNA barcoding analysis. Method: An HPLC method was applied to determine the contents of gentiopicroside, swertiamarin, sweroside and amarogentin in G. rigescens roots and rhizomes, stems, leases, and flowers, and the HPLC fingerprint was established, the main secondary metabolites were evaluated by one-way ANOVA, and the DNA barcoding technique was used to test ITS2 sequences of G. rigescens. Result: There were significant differencedifferences in the contents of gentiopicroside, swertiamarin, sweroside and amarogentin (P<0.01) in different parts.fractions. The results of ITS2 sequences showed that there was no significant difference in the molecule of G. rigescens from different populations. Conclusion: There was differences in main secondary metabolites of G. rigescens from different populations, but there was little difference of with less differences among molecules and a close genetic relationship.  
        关键词：Gentiana rigescens;HPLC;secondary metabolitesmetabolite;ITS2 sequencessequence;DNA barcoding   
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        发布时间：2024-01-04
      • LIU Dong-shun,CHEN Chun-lin,CHE Xiao-hang,TANG Lin-yan,CHEN Li,XU Li-li,XIONG Xiao-juan
        Vol. 22, Issue 18, Pages: 40-44(2016) DOI: 10.13422/j.cnki.syfjx.2016180040
        摘要：Objective: To study the antioxidant activity of Chrysanthemum morifolium flowers, leaves and stems in vitro. Method: Chlorogenic acid was used for the control group (CHA). Extractives were extracted from C. morifolium flowers, leaves and stems. Water and ethanol were used as solvents. These extracts were divided into six groups: flower water extracts (JH-S) group; flower alcohol extracts (JH-C) group, leaf water extracts (JY-S) group, leaf alcohol extracts (JY-C) group, stem water extracts (JJ-S) group; and stem alcohol extracts (JJ-C) group. The antioxidant activity of different extracts were studied by determining the reducing capacity, and the clearance rates of hydroxyl (·OH) radical,1,1-diphenyl-2-pieryl-2-picrylhydrazyl (DPPH·) radical, lipid free radical and superoxide anion (O2-·) radical. The antioxidant activity of chlorogenic acid was compared with that of the extracts. Result: The anti-oxygenation of extracts from C. morifolium flowers, leaves and stems have positively correlated with the concentration, which was the same as chlorogenic acid. There had no difference between ethanol extracts and water extracts from C. morifolium flowers, leaves and stems in reducing capacity; the ethanol extracts and water extracts show a high efficiency in clearing·OH,O2-·radical, and water extracts had a better efficiency than ethanol extracts, with an obviously better effect in resisting O2-·than chlorogenic acid. Ethanol extracts of C. morifolium flowers and leaves were better than its water extracts in cleaning DPPH and lipid free radical ability. The efficiency in clearing DPPH·of ethanol extracts from C. morifolium stems was higher than that of chlorogenic acid. The cleaning lipid free radical function of extracts from C. morifolium leaves was better than that of extracts from C. morifolium flowers with the same concentration. Conclusion: Extracts of C. morifolium flowers, leaves and stems shows an efficient antioxidant activity. The anti-oxygenation function of leaves was equal with flowers. As natural antioxidants, C. morifolium leaves and stems have a great development and utilization value in the future.  
        关键词：Chrysanthemum morifolium flowers;Chrysanthemum morifolium leaves;Chrysanthemum morifolium stems;chlorogenic acid;antioxidant activity   
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        发布时间：2024-01-04
      • Vol. 22, Issue 18, Pages: 45-50(2016) DOI: 10.13422/j.cnki.syfjx.2016180045
        摘要：Objective: To prepare the solid phase extraction column for the effective separation and enrichment of glycyrrhizic acid. Method: A novel surface molecularly imprinted polymer was synthesized, with acrylamide(AM)-modified multi-walled carbon nanotubes(MWCNTs) as supporting matrix, glycyrrhizic acid as template molecule, acrylamide(AM) as functional monomer, N,N-dimethyl formamide(DMF) as pore former and ethylene glycol dimethacrylate(EGDMA) as cross-linking agent. The multi-walled carbon nanotubes molecularly imprinted polymers were characterized by FTIR, SEM and TGA. Result: A stable even layer was imprinted on the MWCNTs surface when the precipitation polymerization temperature was 60℃, the pore forming agent was DMF, EGDMA and solvent ratio was 1:20.The Scatchard model shows that MWCNTs-molecularly imprinted polymers(MIP) had two kinds of binding sites with different affinities and specific recognition ability of glycyrrhizic acid(Kd 1.17 mmol·L-1, Qmax 741.5 μg·g-1, Kd 3.96 mmol·L-1, Qmax 1 668.5 μg·g-1). Conclusion: The molecularly imprinted polymers synthesized with optimal conditions have a good morphological structure and adsorption efficiency, so these polymers as solid-phase extraction material have a certain research value in separation and enrichment of glycyrrhizic acid in medicinal herbs.  
        关键词：multi-walled carbon nanotube;glycyrrhizic acid;molecularly imprinted polymer;adsorption rate   
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        发布时间：2024-01-04
      • LIU Zi-han,LI Chun,YAN Ren-yi
        Vol. 22, Issue 18, Pages: 51-55(2016) DOI: 10.13422/j.cnki.syfjx.2016180051
        摘要：Objective: To study the polyhydroxylated alkaloids of Polygonati Odorati Rhizoma with hypoglycemic effect. Method: The water extract of Polygonati Odorati Rhizoma was isolated and purified by various ion-exchange resins chromatography. The structures of isolated compounds were identified by using spectral analysis such as NMR and MS. The inhibitory activities on α-glucosidase and α-amylase of the isolated alkaloids were screened by PNPG and DNS methods. Result: Eight compounds were obtained from the water extract of Polygonati Odorati Rhizoma, including two polyhydroxy alkaloids, two acylamides, three amino acids and betaine. These isolates were identified as 1-deoxynojirimycin (1), fagomine (2), L-azetidine-2-carboxylic acid (3), 1,2 (S)-pyrrolidinedicarboxamide (4), (2S)-citrullinamide (5), serine (6),-aminobutyric acid (7) and betaine (8). Compounds 1, 2, 4, 5 and 8 were evaluated for their inhibitory activity on α-glucosidase and α-amylase. Compounds 1 and 2 showed potent inhibitory effect on α-glucosidase, with IC50of 0.098, 0.272 mol·mL-1, respectively, whose activities were superior to the positive control Acarbose (IC50of 0.493 mol·mL-1). Furthermore, compound 1 showed inhibitory effect on α-amylase (IC50of 0.681 mol·mL-1), but its inhibitory activity was inferior to Acarbose (IC50 of 0.035 mol·mL-1). Conclusion: Compounds 1-8 were isolated from this plant for the first time, and compounds 1 and 2 showed significant inhibitory effect on α-glucosidase. Therefore, polyhydroxylated alkaloids are the active components of Polygonati Odorati Rhizoma for exerting hypoglycemic effect.  
        关键词：Polygonati Odorati Rhizoma;polyhydroxy alkaloids;α-glucosidase;α-amylase   
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        发布时间：2024-01-04
      • YU Jiang-yong,HUANG Qi-yun,ZHOU Su-juan,CHEN Qiu-fang
        Vol. 22, Issue 18, Pages: 56-59(2016) DOI: 10.13422/j.cnki.syfjx.2016180056
        摘要：Objective: To evaluate and compare the quality of Zingiberis Rhizoma from different batches and different origins by the grey correlation-TOPSIS method. Method: With the contents of 6-gingerol, 8-gingerol, 6-shogaol, diacetoxy-6-gingerdiol and 10-gingerol in Zingiberis Rhizoma of different batches and different origins as the evaluation basis, grey correlation-TOPSIS comprehensive evaluation model was established by using MATLAB programming method, and the quality evaluation of Zingiberis Rhizoma from different batches and different origins was ranked. Result: The grey correlation-TOPSIS model evaluation results indicated that the quality of Sichuan Jianwei 20131205, Guangdong Yangjiang 20140221, Guangdong Zhanjiang 20140125, Anhui Bozhou 20131226 was better than that of other batches, consistent with the traditional evaluation results and genuineness, indicating a good repeatability and stability. Conclusion: Grey correlation-TOPSIS comprehensive evaluation method can be well applied to the quality evaluation of traditional Chinese medicine with multiple components, and have the broad prospect in application.  
        关键词：Zingiberis Rhizoma;grey correlation-TOPSIS;quality evaluation   
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        发布时间：2024-01-04
      • GAO Yan-ping,WU Qiang,LIANG Jian,ZHONG Guo-yue
        Vol. 22, Issue 18, Pages: 60-63(2016) DOI: 10.13422/j.cnki.syfjx.2016180060
        摘要：Objective: To investigate the chemical constituents of Corydalis hendersonii. Method: The constituents were extracted by using systematic solvents, separated and purified by repeated silica gel column chromatography, Sephadex LH-20 column chromatography, semi-preparative HPLC, etc. The structures of the obtained constituents were identified by using NMR, MS and other spectral methods as well as literature data. Result: Thirteen compounds were obtained and identified as apigenin(1), quercetin(2), luteolin(3), kaempferol(4), 5, 7, 4'-trihydroxy flavanone(5), rutin(6), quercitrin(7), astragalin(8), ursolic acid(9), oleanic acid(10), stigmasterol(11), β-sitosterol(12), and daucosterol(13). Conclusion: All the components were obtained from Corydalis hendersonii for the first time.  
        关键词：Corydalis hendersonii;chemical constituents;flavones;triterpenoids;steroids   
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        发布时间：2024-01-04
      • XU Bing-bing,HUANG Bi-tao,ZENG Jin-xiang,LI Min,WANG Juan,ZHU Yu-ye,REN Gang,ZHU Ji-xiao,LUO Guang-ming,WANG Xiao-yun
        Vol. 22, Issue 18, Pages: 64-67(2016) DOI: 10.13422/j.cnki.syfjx.2016180064
        摘要：Objective: To analyze and compare the content differences of verbascoside and isoverbascoside in Plantaginis Semen and Plantaginis Herba, and provide experimental basis for quality control and clinical application of plantain. Method: A RP-HPLC method was applied for the content determination with a Diamonsil C18 column (4.6 mm×250 mm, 5 μm) at 25℃. Methanol-0.1% formic acid aqueous solution(40:60) was used as mobile phase; the flow rate was 0.6 mL·min-1, and the detection wavelength was set at 330 nm. Result: Content of verbascoside in Plantaginis Semen was 9.70 mg·g-1 and 1.28 mg·g-1, respectively; and isoverbascoside was 0.32 mg·g-1 and 1.51 mg·g-1, respectively in Plantaginis Herba. Conclusion: Content of verbascoside in Plantaginis Semen was higher than that of Plantaginis Herba, while the content of isoverbascoside was below than that of Plantaginis Herba. The content differences shall be seriously considered in clinical application. It is recommended to add isoverbascoside as a mark component for the quality control of Plantaginis Semen and Plantaginis Herba.  
        关键词：Plantaginis Semen;Plantaginis Herba;verbascoside;isoverbascoside;RP-HPLC   
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        发布时间：2024-01-04
      • LI Yue-hui,WANG Yin,PENG Yu,YANG Zai-jiang,XIANG Li
        Vol. 22, Issue 18, Pages: 68-71(2016) DOI: 10.13422/j.cnki.syfjx.2016180068
        摘要：Objective: To establish the HPLC fingerprint of Yinsangju solid beverage. Method: Ten batches of Yinsangju solid beverage were analyzed by HPLC method on Kromasil C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.4% phosphoric acid solution as the mobile phase for gradient elution. The detection wavelength was 268 nm; flow rate was 1.0 mL·min-1; column temperature was 35℃ and the injection volume was 10 μL. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(version 2.0) was used to identify common peaks and evaluate similarity. Result: The HPLC fingerprint of Yinsangju solid beverage was established. Twenty common peaks were identified with similarity of more than 0.9 for 10 batches of samples. Three peaks were identified as chlorogenic acid, liquiritin, and luteolin glycosides. Conclusion: This reliable, available and quick method can be used in the quality evaluation of Yinsangju solid beverage.  
        关键词：Yinsangju solid beverage;fingerprint;chlorogenic acid;liquiritin;luteolin glycosides   
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        发布时间：2024-01-04
      • LI Jin,YANG Pan-pan,TANG Wen-xu,LI Hui-juan,XIANG Wan,JIANG Huan,YANG Shi-hua,LI Wei,HOU Ying
        Vol. 22, Issue 18, Pages: 72-77(2016) DOI: 10.13422/j.cnki.syfjx.2016180072
        摘要：Objective: To improve the precision and accuracy of near-infrared spectroscopy(NIR) calibration model for determination of baicalin content in Xiaochaihu granules. Method: Four NIR characteristic spectrum selection methods were used including competitive adaptive reweighted sampling method(CARS), monte carlo uninformative variables elimination(MC-UVE), genetic algorithm(GA) and subwindow permutation analysis(SPA). Model population analysis(MPA) combined with partial least squares(PLS) was used to build five types of models, including CARS-PLS, MCUVE-PLS, GA-PLS, SPA-PLS and all NIR wavelength-PLS. Four model parameters including root mean square error of calibration(RMSEC), root mean square error of prediction(RMSEP), prediction mean relative error(PMRE) and the capability of model interpretation for testing set(Q2) were analyzed to evaluate the precision and accuracy of those NIR calibration models. Result: precision and accuracy of the calibration models: CARS > MC-UVE > GA > all NIR wavelength > SPA. The values of RMSEP and decisive coefficients of the calibration models were 1.700 4 and 0.908 7 respectively, whose characteristic spectrum had been selected by CARS. According to the result of paired-t tests at the level of α of 0.05, there was no significant difference between prediction values and measure values in 50 samples. Conclusion: CARS algorithm to screen wavelength can be used to effectively simplify the models, and improve the precision and accuracy of the models, suitable for fast and nondestructive detecting the baicalin content of Xiaochaihu granules.  
        关键词：Xiaochaihu granules;baicalin;NIR technology;spectrum selection;calibration model   
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        发布时间：2024-01-04
      • MA Da-long,ZHANG Xiao-ping,WEI Bao-hong,ZHANG Peng,MA Xiao-qing,MENG Fan-gang,LIU Hong-bing,DAI Long
        Vol. 22, Issue 18, Pages: 78-81(2016) DOI: 10.13422/j.cnki.syfjx.2016180078
        摘要：Objective: To establish the chromatographic fingerprint for Shaoyao Tang by RP-HPLC. Method: The fingerprint was determined by Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 μm), with the column temperature 25℃, the flow rate 1.0 mL·min-1, the detective wavelength 230 nm and gradient elution with acetoniteile-0.05% phosphoric acid as mobile phase. Using paeoniflorin peak as reference, the HPLC fingerprints of 10 batches of Shaoyao Tang were analyzed. Result: Thirteen common peaks were marked and attributed to the herbs, and 3 common peaks were determined by the relative time of the standard substance, they are paeoniflorin, baicalin and berberine respectively. The HPLC fingerprint of Shaoyao Tang was established and the similarity of the 10 batches of samples was above 0.96. Conclusion: The established HPLC-DAD fingerprint is accurate, stable and reproducible, which could reflect the overall characteristics of Shaoyao Tang and improve the specificity for quality standard of the Chinese herbal formula.  
        关键词：Shaoyao Tang;HPLC-DAD;fingerprint;paeoniflorin   
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        发布时间：2024-01-04
      • LIU Ting,DI Liu-qing,LI Juan,KANG An,LI Jun-song,ZHAO Xiao-li,SHAN Jin-jun
        Vol. 22, Issue 18, Pages: 82-87(2016) DOI: 10.13422/j.cnki.syfjx.2016180082
        摘要：Objective: A rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed and validated for simultaneously determination of phenolic acids and flavonoids in rat plasma after oral administration of Shuanghuanglian preparation. Method: Plasma samples were pretreated by n-butanol-ethyl acetate(1:1).The separation was preformed on an Waters Acquity HSS T3 C18 column(2.1 mm×100 mm,1.7 μm) and the flow rate was set at 0.4 mL·min-1 with mobile phase of acetonitrile-methanol(4:1)-0.4% formic acid for gradient elution.The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring(MRM) via electrospray ionization(ESI) source with positive mode. Result: All calibration curves had good linearity(R2>0.992).RSDs of precision and accuracy were all satisfied.After oral administration of Shuanghuanglian preparation,a remarkable increase in values of AUC0-t and AUC0-∞ of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,baicalin,oroxylin A-7-O-β-D-glucuronide,wogonoside and baicalein was observed by compared with Lonicerae Japonicae Flos extract group or Scutellariae Radix extract group;but the elimination rate of phenolic acids and flavonoids in Shuanghuanglian preparation was slower than the single herb extract. Conclusion: The developed method is successfully applied to pharmacokinetic study of nine active ingredients in Shuanghuanglian preparation,this study can provide experimental evidence for elucidating rationality of compatibility of this preparation.  
        关键词：Shuanghuanglian preparation;phenolic acids;flavonoids;pharmacokinetics;tinidazole   
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        发布时间：2024-01-04
      • SHI Sheng-ying,LIN Hai-zhen,ZHOU Wei,WANG Su-li,LIN Jing-ming
        Vol. 22, Issue 18, Pages: 88-93(2016) DOI: 10.13422/j.cnki.syfjx.2016180088
        摘要：Objective: To investigate the effects of Jiawei Sijunzi decoction containing serum on proliferation, apoptosis, and cell cycle of human hepatocellular carcinoma Hep-G2 cells and explore its action mechanism. Method: After Hep-G2 cells were processed with different concentrations of Jiawei Sijunzi decoction containing serum, cell counting kit-8(CCK-8) was used to measure the proliferation of Hep-G2 cells, apoptosis rate and cell cycle were detected by AnnexinV/propidium iodide(PI) flow cytometry. Apoptotic morphology was determined by hoechst33342 staining. The expression levels of related proteins of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR) signaling pathway in Hep-G2 cells were evaluated by Western blot assay. Result: Jiawei Sijunzi decoction containing serum inhibited the proliferation and induced apoptosis of human hepatoma Hep-G2 cells in a dose-dependent manner. Cells were arrested in G0/G1 phase. Hoechst33342 staining results showed prominent morphological changes with chromatin condensation, fragmentation and formation of apoptotic bodies in dose-dependent manner. Western blot analysis showed that Jiawei Sijunzi decoction containing serum could inhibit phosphorylation of Akt, mTOR, ribosomal S6 kinase(S6), and eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), up-regulate the expression levels of Bax, and down-regulate the expression levels of CyclinD1, Bcl-2. Jiawei Sijunzi decoction containing serum and PI3K/mTOR dual inhibitor VS-5584 had a synergism effect, and Jiawei Sijunzi decoction containing serum enhanced the inhibitory effects on phosphorylation of Akt and mTOR. Conclusion: Jiawei Sijunzi decoction containing serum could inhibit the proliferation of Hep-G2 cells, induce apoptosis and arrest Hep-G2 cells in G1 phase. This inhibitory effect may be induced by blocking the PI3K/Akt/mTOR pathway.  
        关键词：Jiawei Sijunzi decoction;containing serum;Hep-G2 cells;proliferation;apoptosis;phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR) signaling pathway   
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        发布时间：2024-01-04
      • REN Jin-jin,ZHU Ling,WANG Song-lu,GAO Jie,LUO Song-ping,XU Li-mian
        Vol. 22, Issue 18, Pages: 94-99(2016) DOI: 10.13422/j.cnki.syfjx.2016180094
        摘要：Objective: To study the role of anti-mullerian hormone(AMH) in immune premature ovarian failure(POF), investigate the effects of Zuogui Wan(ZGW), which is the kidney tonifying recipe, on AMH and its signal transduction pathway AMH/ALK2/Smad1 in POF mice, and explore the mechanism of ZGW on treating immune POF. Method: The 8 weeks old Balb/c female mice were randomly divided into normal group, model group, Estradiol valerate(trade name: progynova) group and ZGW group. POF animal models were established by zona pellucida 3 polypeptides and then were treated with ZGW or positive control medicine of progynova. The levels of AMH and follicule-stimulating hormone(FSH) in serum of mice were detected by electrochemical luminescence method.The follicle growth rate of the mice was detected by optical microscope. The expression levels of AMH, ALK2 and Smad1 in mouse ovary were detected by immunohistochemistry and Western blot assay. Result: As compared with the normal group, the AMH level was decreased and FSH level was increased in serum of model group mice(P<0.05). As compared with the model group, the AMH level was increased and FSH level was decreased in serum of ZGW group(P<0.05). As compared with the normal group, follicle growth rate was significantly decreased in model group mice(P<0.01). As compared with the model group, follicle growth rate was increased in ZGW group(P<0.05). As compared with the normal group, the protein expression levels of AMH, ALK2 and Smad1 were decreased in mice ovarian of model group(P<0.05). As compared with the model group, the protein expression levels of AMH were increased in mice ovarian of ZGW group(P<0.05); the expression levels of ALK2 and Smad1 were increased in the immunohistochemistry method, but the difference was not significant; the expression levels of both ALK2 and Smad1 were significantly increased in Western blot assay(P<0.05). Conclusion: The occurrence of POF was related to the insufficient secretion of AMH and poor expression of ovarian AMH protein. It affected the primordial follicle recruitment by affecting AMH/ALK2/Smad1 signal transduction, leading to fast follicle consumption, premature ovarian failure, and ovarian aging. ZGW can promote the secretion of AMH in POF mice, up-regulate AMH protein expression in ovary, inhibit the rapid collection and consumption of primordial follicles and improve ovarian function by affecting AMH/ALK2/Smad1 signal transduction.  
        关键词：premature ovarian failure;Zuogui Wan;anti-mullerian hormone;signal transduction   
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        发布时间：2024-01-04
      • AN Zhen-xiang,HE Yuan-li,WANG Min
        Vol. 22, Issue 18, Pages: 100-105(2016) DOI: 10.13422/j.cnki.syfjx.2016180100
        摘要：Objective: To observe the effect of Fupi Rougan formula granule on liver tissue inducible nitric oxide synthase(iNOS) expression in hepatic fibrosis rats and explore its action mechanism. Method: SD rats were randomly divided into normal group, model group, colchicine group(0.2 mg·kg-1), Fuzheng Huayu group(0.415 g·kg-1), Fupi Rougan low-dose(20 g·kg-1) group, Fupi Rougan middle-dose(40 g·kg-1) group, and Fupi Rougan high-dose(80 g·kg-1) group. SD rat models of hepatic fibrosis were induced by carbon tetrachloride composite ethanol(CCl4). After 8 weeks, the rats in various groups were given with corresponding drug intervention respectively by intragastric administration, once daily for 4 weeks. The rats in normal group and model group were given with normal saline. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and hyaluronic acid(HA) of rat serum were detected at the end of 12th week. HE staining was used to observe the pathological changes in liver tissue. Fluorescence Real-time PCR and Western blot assay were used to detect mRNA and protein expression levels of iNOS in rat liver tissues. Result: As compared with normal group, the contents of ALT, AST and HA in rat serum were significantly increased in model group(P<0.01). As compared with model group, the contents of ALT and AST in colchicine group, Fuzheng Huayu group, Fupi Rougan low-dose group and Fupi Rougan middle-dose group were decreased(P<0.05), the contents of ALT and AST in Fupi Rougan high-dose group were significantly increased(P<0.01), the contents of HA in rat serum of colchicine group, Fuzheng Huayu group and Fupi Rougan middle-dose group were decreased(P<0.05), the content of HA in Fupi Rougan high-dose group was significantly decreased(P<0.01). As compared with model group, the liver tissues fibrosis was relieved in various degrees in each drug intervention group(P<0.05). As compared with normal group, the mRNA and protein expression levels of the rat liver tissue iNOS were significantly increased in model group (P<0.01). As compared with model group, the mRNA and protein expression levels of the rat liver tissue iNOS were significantly decreased(P<0.01) in each drug intervention group, especially in Fupi Rougan high-dose group(P<0.01). Conclusion: The Fupi Rougan formula granule can reduce the mRNA and protein expression levels of the liver fibrosis tissue iNOS. It may be one of the anti-hepatic fibrosis mechanisms.  
        关键词：Fupi Rougan formula granule;hepatic fibrosis;inducible nitric oxide synthase;alanine aminotransferase;aspartate aminotransferase;hyaluronic acid   
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        发布时间：2024-01-04
      • FEI Hong-xin,ZHANG Ying-bo,ZHANG Xiao-jie,BAI Qing-yang
        Vol. 22, Issue 18, Pages: 106-111(2016) DOI: 10.13422/j.cnki.syfjx.2016180106
        摘要：Objective: To observe the effects of phenolic alkaloids of Menispermum dauricum(PAMD) on CCl4 induce hepatic fibrosisin rats and investigate its action mechanism. Method: Sprague-Dawley rats were randomly divided into normal group, model group, colchicine group(1 mg·kg-1), PAMD high-dose group(25 mg·kg-1). PAMD medial-dose group(12.5 mg·kg-1) and PAMD low-dose group(6.25 mg·kg-1). Intraperitoneal injection of 40% CCl4(1 mL·kg-1) was used to induce hepatic fibrosis models in the rats. All the rats received the same volume of drugs or normal saline by intragastric administration for 30 days. The radioimmunoassay was used to determine the levels of hyaluronic acid(HA), laminin(LN), procollagen Ⅲ(PCⅢ) and collagen Ⅳ(CⅣ) in serum. The biochemical analysis was used to determine the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST), total protein(TP) and album protein(ALB) in serum. The enzyme-linked immuno sorbent assay(ELISA) was applied to detect the levels of transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in the liver tissues. Morphology of liver tissues was observed by optical electron microscopy, and the organ coefficients of liver and spleen were calculated then. Result: As compared with the normal group, the levels of HA, LN, PCⅢ, CIV, ALT and AST in serum were significantly increased in the serum of model group(P<0.05); levels of TP and ALB in serum were significantly decreased(P<0.05); levels of TGF-β1 and TNF-α in the liver tissues were significantly increased(P<0.05); the liver pathological fibrosis was aggravated significantly; organ coefficients of liver and spleen were significantly increased(P<0.05). As compared with the model group, the levels of HA, LN, PC-Ⅲ, CIV, ALT and AST in serum were significantly decreased in PAMD high-dose group and PAMD medial-dose group(P<0.05); the levels of TP and ALB in serum were significantly increased(P<0.05); levels of TGF-β1 and TNF-α in the liver tissues were significantly decreased(P<0.05); the liver pathological fibrosis was obviously relieved; organ coefficients of liver and spleen were significantly decreased(P<0.05). Conclusion: PAMD could attenuate the CCI4-induced immunological liver injury and the fibrosis level in rats. The mechanisms possibly contribute to down-regulating the expression levels of TGF-β1 and TNF-α proteins in liver tissues of rats.  
        关键词：phenolic alkaloids of Menispermum dauricum;hepatic fibrosis;carbon tetrachloride;transforming growth factor-β1;tumor necrosis factor-α   
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        发布时间：2024-01-04
      • FANG Xiao-yan,WANG Lin-lin,ZHANG Fan,SUN Jian-ning,MIAO Ming-san
        Vol. 22, Issue 18, Pages: 112-117(2016) DOI: 10.13422/j.cnki.syfjx.2016180112
        摘要：Objective: To observe the neuroprotective effects of cerebral ischemic preconditioning (CIP) on cerebral ischemia reperfusion and the expressions of brain-derived neurotrophic factor(BDNF), grial cell-lime derived neurotrophic factor(GDNF) and vascular endothelial growth factor(VEGF) in the cortex and hippocampus CA1 area of rats. Method: Wistar rats were randomly divided into three groups, Sham operation group, cerebral ischemia reperfusion (I/R) group, and cerebral ischemic tolerance (CIP+MCAO) group. The behavioral changes were detected by neurologic deficit scores (NDS), the cerebral histopathology was detected by HE staining, and the level of NSE in serum were detected by ELISA. The expressions of BDNF, GDNF and VEGF in cortex, hippocampus CA1 area were measured by immunohistochemical staining. Result: Compared with Sham operation group, I/R group showed significant increases in neurologic deficit scores and the level of NSE in serum, and improved cerebral histopathology injury (P<0.01), notable decreases in positive area and integral absorbance (IA) in cortex and hippocampus CA1 area at the damage side, but only statistical differences between brain cortex and Sham operation group; Compared with I/R group, CIP+MCAO decreased the neurologic deficit scores (P<0.05), cerebral histopathology injury (P<0.05), and NSE in serum (P<0.01). At the same time, the positive area and integral absorbance (IA) of BDNF, VEGF in cortex, and CA1 area at the damage side increased obviously (P<0.01, P<0.05). The positive expression of GDNF showed an increasing trend, but with no statistical difference. Conclusion: The neuroprotective effects of CIP were correlated with the up-regulation of the endogenous proteins BDNF and VEGF.  
        关键词：cerebral ischemic preconditioning;cerebral ischemiareperfusion;cerebral ischemic tolerance;neurotrophic factor;endogenous protective effect   
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        发布时间：2024-01-04
      • LIU Jiang-yue,ZHANG Dai-juan,LI Wen-tao,GUO Jun-tang
        Vol. 22, Issue 18, Pages: 118-122(2016) DOI: 10.13422/j.cnki.syfjx.2016180118
        摘要：Objective: To observe the effect of catalpol on oxidized low density lipoprotein(ox-LDL) induced inflammatory cytokine in EA.hy926 cells and to explore its possible mechanism from the prospective of nuclear factor-κB(NF-κB) activation. Method: Humane endothelial cell line EA.hy926 was cultured and randomly divided into blank group, catalpol group(0.5 mmol·L-1), ox-LDL group(100 mg·L-1), catalpol high-dose group(0.5 mmol·L-1), and low-dose group(0.05 mmol·L-1). The mRNA and protein expression levels of tumor necrosis factor α(TNF-α) and vascuolar cell adhesion molecule-1(VCAM-1) in EA.hy926 cells were detected by RT-PCR and Western blot respectively. Viability and apoptosis ratio of EA.hy926 cells were detected by MTT and Hoechst staining respectively. NF-κB activation was detected by Western blot and ELISA. Result: As compared with the blank group, EA.hy926 cells were injured very slightly in catalpol protection group, and the mRNA and protein expression levels of TNF-α and VCAM-1 were significantly reduced and NF-κB activation was inhibited in a dose-dependent manner(P<0.05). Therefore, catalpol can effectively reduce ox-LDL-induced inflammatory response in EA.hy926 cells, which may be related to inhibition of NF-κB activation.  
        关键词：catalpol;inflammatory factors;nuclear factor-κB;tumor necrosis factor-α;vascular cell adhesion molecule-1   
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        发布时间：2024-01-04
      • PENG Jing,CHEN Jing,TIAN Shou-zheng,XING Hai-jing,YUAN Jia-li
        Vol. 22, Issue 18, Pages: 123-126(2016) DOI: 10.13422/j.cnki.syfjx.2016180123
        摘要：Objective: To observe the effects of different doses of modified Yupingfeng San on Haemophilus influenza, Klebsiella pneumoniae and Pseudomonas aeruginosa in airway of chronic obstructive pulmonary disease(COPD) rats, and provide experimental basis for the treatment of COPD by modified Yupingfeng San. Method: COPD rat models were induced by intratracheal instillation of LPS plus smoked method. The Wistar rats were randomly divided into normal group, model group, modified Yupingfeng San high dose, middle dose and low dose groups(4.644,9.288,27.864 g·kg-1·d-1), n=10 in each group. The contents of 3 kinds of bacteria were analyzed by high-throughput sequencing method in each group, and the histopathological changes of the right lung were observed by HE staining. Result: As compared with the normal group, the contents of H. influenza, K. pneumoniae and P. aeruginosa were increased in the airway of model group mice(P<0.05); right lung pathological observation results showed increase of chronic inflammatory cells in right lungs of model group and a small amount of neutrophil infiltration. As compared with the model group, the contents of H. influenza, K. pneumoniae and P. aeruginosa were decreased in modified Yupingfeng San low dose and middle dose groups(P<0.05); the contents of the above bacteria were decreased in modified Yupingfeng San low dose, middle dose and high dose groups(P<0.05, P<0.01), and the lesions in right lung tissues were significantly improved. Conclusion: Modified Yupingfeng San could significantly reduce the contents of H. influenza, K. pneumoniae and P. aeruginosa in airway of COPD rats, and the low dose group had more obvious effects on P. aeruginosa. This may be one of the important mechanisms for the treatment of COPD.  
        关键词：modified Yupingfeng San;chronic obstructive pulmonary disease;Haemophilus influenza;Klebsiella pneumoniae;Pseudomonas aeruginosa   
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        发布时间：2024-01-04
      • LI Guang-quan,LIU Hui-min,CUI He-rong,ZHANG Zhen-fang,SANG Xiu-xiu,WANG Jia-kui,BAI Zhao-fang
        Vol. 22, Issue 18, Pages: 127-131(2016) DOI: 10.13422/j.cnki.syfjx.2016180127
        摘要：Objective: To observe the therapeutic effects of Gantai capsules(GTC) on carbon tetrachloride(CCl4) induced liver fibrosis in rats, and investigate its action mechanism. Method: Liver fibrosis in rats was induced by intraperitoneal injection of CCl4 for 8 weeks. The SD rats were randomly divided to model group, the positive colchicine group(2 mg·kg-1), and GTC low dose and high dose groups(230, 460 mg·kg-1), n=12 in each group. In addition, another 12 rats were selected from the same batch as normal group. The rats in normal and model groups were administrated with an equal volume of normal saline, while the rats in other groups were treated with corresponding substances at a given dose byig administration, 1 time/day for 4 weeks. At the end of this experiment, all rats were sacrificed, and their blood and liver tissues were obtained for research. The activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were detected by colorimetry; the levels of hyaluronic acid(HA), laminin(LN), procollagen Ⅲ(PC-Ⅲ) and collagen IV(CIV) in serum were detected by ELISA; the contents of hydroxyproline(HyP) in liver tissues were detected after alkali-hydrolysis; the liver tissues were stained by using HE and Masson staining; histopathological changes were observed under optical microscope. Result: As compared with the normal group, the levels of ALT, AST, HA, LN, CⅣ and PCⅢ in serum were significantly increased in model group; content of HyP in liver tissues was significantly increased(P<0.01); the liver pathological injury was obvious with more collagen deposition in the liver tissues. As compared with the model group, the levels of ALT, AST, HA, LN, CⅣ and PCⅢ in serum were significantly decreased in GTC groups(P<0.05, P<0.01); the contents of HyP in liver tissues was decreased(P<0.05, P<0.01). Pathological results showed that GTC could obviously improve the liver pathological injury and reduce collagen deposition in the liver tissues. Conclusion: GTC could effectively attenuate the CCl4-induced liver fibrosis in rats.  
        关键词：Gantai capsules;carbon tetrachloride;liver fibrosis;hyaluronic acid;laminin;Collagen Ⅳ;procollagen Ⅲ;hydroxyproline   
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        发布时间：2024-01-04
      • XIAO Huan-ming,WU Hui-jun,XIE Yu-bao,CHI Xiao-ling
        Vol. 22, Issue 18, Pages: 132-135(2016) DOI: 10.13422/j.cnki.syfjx.2016180132
        摘要：Objective: To investigate the clinical effect of Hehe Shuyang formula(HHSYF) in treatment of carbon tetrachloride (CCl4) induced hepatic injury and liver fibrosis in rats, and provide experimental basis for the clinical treatment of liver fibrosis. Method: By orthogonal test, 40 SD rats were randomly divided into four groups: normal group, model group, positive drug group(bifendate, 50 mg·kg-1·d-1), and HHSYF group(83 g·kg-1·d-1). Chronic restraint stress+excessive exercise+improper diet were used in the normal group for modeling for 4 weeks, and then ig administration of distilled water to week 8. In the positive drug group, bifendate was given by ig administration till week 8; and ig administration was given in HHSYF group till week 8. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), γ-glutamyltransferase(GGT) levels in serum and malondialdehyde(MDA) levels in liver tissues, as well as the level of hydroxyproline(HyP) were detected; histopathological observation was done by HE staining. Result: As compared with the normal group, the levels of ALT, AST and GGT in serum were significantly increased, and the levels of MDA and HyP in liver tissues were significantly increased in rats of model group(P<0.01); pathological results showed that the liver tissue injury was more obvious in model group. As compared with the model group, the levels of ALT, AST and GGT in serum were significantly decreased, and the levels of MDA and HyP in liver tissues were significantly decreased in rats of HHSYF group(P<0.01), and the liver tissue injury was significantly improved. Conclusion: HHSYF has better effect on improving liver function and reversing hepatic fibrosis.  
        关键词：Hehe Shuyang formula;carbon tetrachloride;treatment;hepatic fibrosis;effect   
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        发布时间：2024-01-04
      • HU Ze-cheng
        Vol. 22, Issue 18, Pages: 136-139(2016) DOI: 10.13422/j.cnki.syfjx.2016180136
        摘要：Objective: To explore the effects of Juglandis Immaturum Exocarpium polysaccharide(JIP) on the proliferation, apoptosis and phosphatidylinositol 3'-OH kinase/protein kinase B(PI3K/Akt) signaling pathway of human colon carcinoma cell line HCT-116. Method: The HCT-116 cells in logarithmic growth phase were treated with JIP(10, 20, 50, 100 mg·L-1), and another blank group was set up. The inhibition rates of proliferation at 24, 48, 72, 96 h post-treatment were measured by MTS assay. The cell cycle and apoptosis rate at 48 h post-treatment were detected by PI single staining and Annexin-FITC/PI double staining methods, and the protein levels of Akt and p-Akt at 48 h post-treatment were detected by Western blot assay. Result: JIP presented significant inhibitory effect on HCT-116 cell proliferation in a dose-and time-dependent manner. As compared with the blank group, the cells ratio in G0/G1 phase was increased and cells ratio in S phase and G2/M phase was declined with significant differences in various groups(P<0.05). As compared with the blank group, the early apoptosis rate of JIP in all the groups except 10 mg·L-1 group was significantly increased, and the apoptosis rate of late phase and total apoptosis rate in various groups were significantly increased with statistical significance(P<0.05). As compared with the blank group, the ratio of p-Akt/Akt was decreased in various treatment groups, and there was statistically significant difference in ratio of p-Akt/Akt between various concentrations(P<0.05). Conclusion: JIP can directly inhibit or kill human colon cells HCT-116 cellsin vitro, and present ability to inhibit proliferation and induce apoptosis. The mechanism might associated with PI3K/Akt signaling pathway.  
        关键词：Juglandis Immaturum Exocarpium polysaccharide;colon carcinoma;proliferation;apoptosis;phosphatidylinositol 3'-OH kinase/protein kinase B   
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        发布时间：2024-01-04
      • WANG Hong-li,DENG Tian-lian,TANG Xian-ping
        Vol. 22, Issue 18, Pages: 140-143(2016) DOI: 10.13422/j.cnki.syfjx.2016180140
        摘要：Objective: To observe the clinical efficacy of Qingli Huoxue Jiedu Tang on damp-heat accumulation type gouty arthritis, and explore its action mechanism. Method: The 90 patients with damp-heat accumulation type gouty arthritis were randomly divided into treatment group(60 cases) and control group(30 cases). Patients in treatment group were treated with Qingli Huoxue Jiedu Tang(once in the morning and evening by oral taking)+loxoprofensodium tablets(60 mg po, bid), patients in control group were treated with loxoprofensodium (60 mg po, bid). Patients were observed for 2 weeks in both groups. The traditional Chinese medicine(TCM) syndrome scores, and the hours of reducing swelling of joint pain were observed, erythrocyte sedimentation rate(ESR), C-reactive protein(CRP), uric acid(UA), visual analogue scale(VAS), 15 meters walking time, routine blood test, routine urine test, safety indexes of liver and kidney functions and other adverse reactions were recorded. Result: The improvement in TCM syndrome scores, the hours of reducing swelling of joint pain, ESR, CRP, UA, VAS, and 15 meters walking time in treatment group was better than that in control group(P<0.05, P<0.01), and no adverse reaction was present in treatment group. Conclusion: Qingli Huoxue Jiedu Tang can quickly relieve joint swelling, pain and other symptoms, improve joint range of motion, reduce uric acid and inflammatory indexes.  
        关键词：Qingli Huoxue Jiedu Tang;gouty arthritis;damp-heat accumulation;erythrocyte sedimentation rate;C-reactive protein;uric acid   
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        发布时间：2024-01-04
      • LI Qin,WANG Li,MA Lan
        Vol. 22, Issue 18, Pages: 144-148(2016) DOI: 10.13422/j.cnki.syfjx.2016180144
        摘要：Objective: To explore the interventional effects of Fuzheng Kangdu pills combined with highly active antiretroviral therapy(HAART) therapy on oxidative stress in patients with human immunodeficiency virus(HIV) infection stage IIB, and investigate its action mechanism. Method: The 120 patients were randomly divided into blank group, HAART group, and Fuzheng Kangdu pills+HAART group by using the random number table. Complete data were obtained from 94 of these patients, including 30 cases in the blank group, 33 cases in HAART group, and 31 cases in Fuzheng Kangdu pills+HAART, patients in all the groups received treatment for 12 months to observe the effects of Fuzheng Kangdu pills+HAART on reactive oxygen species(ROS), nuclear factor-κB(NF-κB), and absolute counts of the T lymphocyte subsets in patients with HIV infection stage ⅡB. Result: At 12 months of treatment, ROS and NF-κB levels in Fuzheng Kangdu pills+HAART group were significantly lower than those in blank group and HAART group(P<0.05), as compared to blank group, both HAART group and Fuzheng Kangdu pills+HAART group could effectively increase the number of patients with viral load<100 copy·mL-1 (P<0.01) and reduce the number of patients with viral load fluctuation(P<0.01), which however presented no significant differences between the two groups. CD3+ T lymphocytes counts in the Fuzheng Kangdu pills+HAART group were significantly increased as compared with the conditions before treatment, blank group or HAART group(P<0.05, P<0.01). CD4+T lymphocyte counts in HAART group and Fuzheng Kangdu pills+HAART group were significantly increased as compared with the conditions before treatment or blank group(P<0.05), CD4+T lymphocyte count in Fuzheng Kangdu pills+HAART group showed an increasing tendency as compared with that in HAART group. Conclusion: Fuzheng Kangdu pills combined with HAART therapy could reduce oxidative stress and effectively inhibit NF-κB level elevation in patients with HIV infection stage ⅡB, and meanwhile, it could also effectively increase the number of patients with plasma viral load<100 copy·mL-1, reduce the number of patients with viral load fluctuation, and raise CD3+ and CD4+ T lymphocyte count.  
        关键词：Fuzheng Kangdu pills;highly active antiretroviral therapy;human immunodeficiency virus infection stage ⅡB;oxidative stress;interventional effects   
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        发布时间：2024-01-04
      • LIU Can-jun,SHAN Jie,WENG Hai-tao,HUANG Kan
        Vol. 22, Issue 18, Pages: 149-153(2016) DOI: 10.13422/j.cnki.syfjx.2016180149
        摘要：Objective: To investigate the efficacy of Yiqi Jiedu Huxin decoction in treating acute viral myocarditis(VMC) with syndrome of pathogenic toxin invasion heart and observe its effects on levels of high mobility group box1 protein(HMGB1) and nuclear factor-κB(NF-κB). Method: The 152 cases of acute VMC were randomly divided into control group(76 cases) and observation group(76 cases) according to random number table. All patients were treated with non-drug treatment such as staying in bed. Cases in control group were orally treated with Vitamin C tablets(200 mg/time and tid) and Q10 Tablets(1 tablet/time,tid). Based on the treatment in control group, patients in treatment group were given with Yiqi Jiedu Huxin decoction, 1 dose/d and bid. The treatment course was four weeks for both groups. Scores of traditional Chinese medicine(TCM) symptoms were graded before and after treatment, levels of creatine kinase(CK), serum troponinI(cTnI), creatine kinase-MB(CK-MB) were detected before and after treatment. Serum levels of HMGB1 and NF-κB were detected in two groups. Result: Total effective rate was 92.11% in observation group, higher than 80.26% in control group(P<0.05). After treatment, scores of palpitations, chest pain, fever, and sore throat in observation group were lower than those in control group(P<0.01), the levels of CK,cTnI and CK-MB in observation group were significantly lower than those in control group(P<0.01). Serum levels of HMGB1 and NF-κB in observation group were lower than those in control group at week 2 and week 4 after treatment. Conclusion: Based on the conventional western medicine intervention,Yiqi Jiedu Huxin decoction in treating acute VMC with syndrome of pathogenic toxin invasion heart can obviously improve TCM symptoms, reduce the levels of myocardial enzyme, and increase clinical efficacy. Its mechanism may be associated with down-regulating serum levels of HMGB1 and NF-κB.  
        关键词：Yiqi Jiedu Huxin decoction;acute viral myocarditis;pathogenic toxin invasion heart;myocardial enzyme;high mobility group box1 protein;nuclear facter-κB   
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        发布时间：2024-01-04
      • WANG Xiao-li,WANG Cai,CHEN Fan
        Vol. 22, Issue 18, Pages: 154-158(2016) DOI: 10.13422/j.cnki.syfjx.2016180154
        摘要：Objective: To investigate the clinical efficacy of compound Rhodiola A Bor oral solution combined with ambroxol hydrochloride treatment for patients with radioactive lung injury by comparing the application effect of compound Rhodiola A Bor oral solution combined with ambroxol hydrochloride treatment and simple ambroxol hydrochloride treatment. Method: The 116 patients with radioactive lung injury in our hospital from August 2014 to August 2015 were selected and randomly divided into observation group(58 cases) and control group(58 cases) by random number table. Patients of both groups received oral ambroxol hydrochloride tablets, and patients in observation group were also given with oral liquid of compound rhodiola A Bor oral solution based on this. The levels of transforming growth factor-β1(TGF-β1), tumor necrosis factor-α(TNF-α), and changes of Karnofsky(KPS) scores after radiation therapy, after 4 weeks and 8 weeks of treatment, as well as the changes in lung function and the quality of life before and after treatment were observed in both groups. Result: The levels of TGF-β1 and TNF-α of patients in observation group were significantly lower than those of control group after 4 weeks and 8 weeks treatment(P<0.05), and the KPS scores in observation group were significantly higher than those of control group after 4 weeks and 8 weeks treatment (P<0.05). As compared with control group after treatment, the test results of vital capacity(VC), forced vital capacity(FVC), forced expiratory volume in one second(FEV1), FEV1/FVC, the scores of functional areas and scores of overall health in observation group were significantly increased after treatment,while the scores of symptoms and scores of single items were significantly decreased(P<0.05). The total effective rate was 89.66%in observation group, and 60.34%in control group. As compared with the control group after treatment, the number of patients with marked effect and the total effective rate in observation group were significantly increased, and the number of patients with no effects was decreased significantly (P<0.05). Conclusion: The application of compound Rhodiola A Bor oral solution combined with ambroxol hydrochloride treatment for the radioactive lung injury can effectively reduce the levels of TGF-β1 and TNF-α caused by a radiation therapy, improve lung function, and thus improve the clinical efficacy, at the same time with a significant effect to the improvement of life ability and life quality.  
        关键词：radioactive lung injury;traditional Chinese medicine;transforming growth factor-β1;tumor necrosis factor-α;Karnofsky;life quality   
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        发布时间：2024-01-04
      • SONG Bin,SONG Hong,WANG Hong-ru,WANG Wei,HUANG Hua,CUI Jing,WANG Rong,PENG Han-yuan
        Vol. 22, Issue 18, Pages: 159-163(2016) DOI: 10.13422/j.cnki.syfjx.2016180159
        摘要：Objective: To evaluate the effect of Yaoning decoction combined with Shexiang Zhitong patch in treating lumbar muscle strain(cold dampness and blood stasis) and discuss the mechanism of action according to hemorheology and inflammatory response. Method: One hundred and eighty patients were randomly divided into control group(90 cases) and observation group(90 cases) by using a stratified-block randomized method. Patients in control group received diclofenac sodium sustained release tablets by oral taking, 100 mg/time, 1 time/day, and diclofenac diethylamine emulgel for external use. Patients in observation group took Yaoning decoction, 1 dose/day, and Shexiang Zhitong patch for external use. The treatment was 2 weeks for both groups, and the follow-up lasted for 4 weeks. On day 0, 7, 14 and 42, pain scores on visual analogue scale(VAS) were evaluated, and both before and after treatment, scores of Oswestry disability index(ODI) and cold dampness and blood stasis syndrome were graded; levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), thromboxane B2(TXB2), 6-Ketoprostaglandin Fla(6-Keto-PGFla) and hemorheological property were detected before and after treatment. Result: Ridit analysis results showed that the comprehensive clinical efficacy in observation group was superior to that in control group(P<0.05). On day 7, 14 and 42, VAS scores in observation group were lower than those in control group(P<0.01). Individual scores and total scores of ODI index in observation group were lower than those in control group after treatment(P<0.01). The scores of cold dampness and blood stasis syndrome in observation group were lower than those in control group after treatment(P<0.01). The amelioration of hemorheological properties such as whole blood viscosity(high and low shearing), plasma viscosity, fibrinogen and platelet aggregation rate were superior to those in control group(P<0.01). Levels of serum TNF-α, IL-6 and TXB2 in observation group were lower than those in control group, while the level of 6-Keto-PGFla was higher than that in control group(P<0.01). Conclusion: Yaoning decoction combined with Shexiang Zhitong patch can relieve pain, ameliorate waist function and microcirculation disturbance, and reduce inflammatory response for chronic lumbar strain, with obvious comprehensive clinical efficacy.  
        关键词：chronic lumbar strain;Yaoning decoction;Shexiang Zhitong patch;tumor necrosis factor-α;interleukin-6;thromboxane B2;6-ketoprostaglandin Fla;hemorheology   
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        发布时间：2024-01-04
      • ZHEN Yan,LIN Zi,ZHENG Ping,YE Ming-fang
        Vol. 22, Issue 18, Pages: 164-168(2016) DOI: 10.13422/j.cnki.syfjx.2016180164
        摘要：Objective: To discuss the clinical efficacy of Songling Xuemaikang capsule combined with Qiju Dihuang pill in treating elderly hypertension(deficiency of liver and kidney and internal static blood obstruction) and investigate its effects on vascular endothelial function. Method: One hundred and forty-seven patients were randomly divided into western medicine group(74 cases) and combination group(73 cases) by random number table. Patients in western medicine group took felodipine sustained release tablets, 5-10 mg/time, 1 time/day, losartan potassium and hydrochlorothiazide tablets, 1 tablet/time, 1 time/day. Based on the treatment in western medicine group, patients in combination group added Songling Xuemaikang capsule(2 capsules/time, 3 times/day) combined with Qiju Dihuang pill(9 g/time, 2 times/day). The treatment course was 12 weeks for both groups. Before and after treatment, levels of blood pressure were detected to calculate the blood pressure compliance rate, flow-mediated dilation(FMD) was assessed by color doppler ultrasound diagnostic system in brachial artery both before and after treatment; pulse wave velocity(PWV) including brachial-ankle pulse wave velocity(baPWV) and carotid-femoral artery PWV(cfPWV) was detected by automatic arteriosclerosis detector both before and after treatment,traditional Chinese medicine(TCM) syndrome scores were graded, levels of nitric oxide(NO), endothelin-1(ET-1), von Willebrand factor(vWF) and P-selectin were also detected both before and after treatment. Result: The total effective rate for blood pressure in combination group was 95.89% after treatment, higher than 86.49%in western medicine group(P<0.05). Blood pressure compliance rate was 68.49% in combination group higher than 51.35%in western medicine group(P<0.05). After treatment, levels of SBP, DBP, baPWV, cfPWV, ET-1, vWF and P-selectin and TCM syndrome scores in combination group were lower than those in western medicine group(P<0.01), and levels of FMD and NO were higher than those in western medicine group(P<0.01). Conclusion: Songling Xuemaikang capsule combined with Qiju Dihuang pill can control blood pressure level, ameliorate the symptoms of liver and kidney deficiency and blood stasis, improve vascular endothelial function, and reduce the risk of cardiovascular diseases in treatment of elderly hypertension.  
        关键词：hypertension;elderly;Songling Xuemaikang capsule;Qiju Dihuang pill;vascular endothelial function   
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        发布时间：2024-01-04
      • YE Tian-long,ZHOU Shi-gao,WEI Hua-feng
        Vol. 22, Issue 18, Pages: 169-175(2016) DOI: 10.13422/j.cnki.syfjx.2016180169
        摘要：Objective: To mine data and analyze the clinical cases of Professor XIA Xiang, in order to disclose his formula rules in treating cardiovascular and cerebrovascular diseases with Qi-supplementing and blood-activating method, and suggest methods and herbs for this disease. Method: The first-visit cardiovascular and cerebrovascular cases treated by Pro. XIA Xiang with Qi-supplementing and blood-activating method were collected, and the selected cases were entered in the Traditional Chinese Medicine Inheritance Support Platform (V2.5, TCMISS) to analyze the rules of Qi-supplementing and blood-activating method, herbs and symptoms through data mining. Then two disease entities with the largest number of cases were selected to further analyze the association rules of diseases, therapies and herbs. Result: Totally 89 formulas were collected, including 28 cases of headache and palpitation, 17 cases of chest distress, 15 cases of vertigo and 1 case of stroke. The common symptoms in order were fluster, chest distress, headache, dizzy, chest pain, insomnia, chill, fatigue, thirst, alliteration and tinnitus. They involved totally 220 types of herbs in 89 formulas, which were dominated by Qi-supplementing and blood-activating herbs, such as Astragali Radix, Salviae Miltiorrhizae Radix et Rhizoma, Puerariae Lobatae Radix, Codonopsis Radix and Chuanxiong Rhizoma. There were 8 and 15 common herbs treating headache and palpitation with the support score ≥18 and 15, involving 21 and 25 herbal pairs, there were 32 and 15 herbal combinations with the confidence score ≥95%, which produced 8 and 9 new formulas, respectively. Conclusion: TCMISS disclosed Pro. XIA Xiang's formulas rules for treating the cardiovascular and cerebrovascular diseases with the Qi-supplementing and blood-activating method, which helps promote the clinical efficacy of TCM.  
        关键词：Qi-supplementing and blood-activating method;cardiovascular and cerebrovascular diseases;Traditional Chinese Medicine Inheritance Support Platform   
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        发布时间：2024-01-04
      • SHI Hai-long,CUI Ya-ya,LI Jun,DANG Lin,GUO Xin-rong
        Vol. 22, Issue 18, Pages: 176-182(2016) DOI: 10.13422/j.cnki.syfjx.2016180176
        摘要：Objective: To clarify material basis and mechanism of compound Danshen formulae,and provide a reference for clinical application of this compound. Method: Traditional Chinese medicine pharmacological database(TCMSP) was adopted,101 candidate compounds from compound Danshen formulae were screened,a molecular docking program of AutoDock Vina was employed to investigate the interactions of these 101 candidate compounds with eight coronary heart disease targets,namely angiotensin-Ⅰ converting enzyme(ACE),hydroxymethylglutaryl coenzyme A receptor(HMGR),human heat shock protein 90 kinase(HSP90),peroxisome proliferator activated receptor-γ(PPAR-γ),thrombin,vascular endothelial growth factor receptor-Ⅱ(VEGFR2),androgen receptor(AR) and cyclooxygenase-2(COX-2). Result: Danshen-spiroketallactone can interact with six targets,seventeen compounds can interact with two or more targets,and the other five compounds may be potent for at least one of these eight targets. Conclusion: In this study,a preliminary explanation of compound Danshen formulae,Salviae Miltiorrhizae Radix et Rhizoma is a "monarch" medicine,Notoginseng Radix et Rhizoma and Borneolum Syntheticum are used as "minister" medicine and "assistant" medicine,respectively.It gives us a better understanding of the mechanism of this compound.  
        关键词：molecular docking;compound Danshen formulae;coronary heart disease;multi-target;thrombin;angiotensin-Ⅰ converting enzyme   
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      • XU Chun-juan,CHEN Rong,HE Xiao-hui
        Vol. 22, Issue 18, Pages: 183-186(2016) DOI: 10.13422/j.cnki.syfjx.2016180183
        摘要：Objective: Experienced Prescriptions of Ruizhutang was written mostly byShatu Musu,the minority nationality officer,while in office of Jianchang prefecture in the the Yuan dynasty.The local works of traditional Chinese medicine(TCM) are consisted of examining and correcting the famous theories of TCM,collecting folk experience recipes of Xujiang,selecting and preparing drugs carefully,clinically tested and doctors corrected together.But it is regarded as the ethnic minorities works due to the national particularities of the editor-in-chief.This paper is regarded as Xujiang medical works from the aspects of the author's experience,where the book written,the progress of writing and drug characteristics.This paper studies on the prescription characteristics firstly and systematically from five aspects like liking and being good at notification,using the good medicine to treat arthralgia-syndrome,anti-aging and being beautiful,emphasizing use of processing,being handed down with classical prescriptions and so on.  
        关键词：the Yuan dynasty;Shatu Musu;Xujiang medical works;Experienced Prescriptions of Ruizhutang;prescription characteristics   
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        发布时间：2024-01-04
      • WANG Qiu-hong,ZHAO Shan,WANG Peng-cheng,WANG Zhi-bin,KUANG Hai-xue
        Vol. 22, Issue 18, Pages: 187-191(2016) DOI: 10.13422/j.cnki.syfjx.2016180187
        摘要：As a critical link during pharmaceutical process of traditional Chinese medicine (TCM),extraction is directly related to quality of drugs.So the key to technology transformation and upgrading of pharmaceutical equipment in Chinese materia medica enterprise is development of new extraction techniques,which concerns modernization of TCM.Based on grey mode of thinking,semi-bionic extraction mimics human gastroenteric environments to obtain extracts which contain more active compounds.This paper reviewed application of semi-bionic extraction as well as the emerging techniques (microwave,ultrasonic and enzyme) assisted semi-bionic extraction method in TCM extraction,and found remarkable advantages of semi-bionic extraction by comparing with traditional extraction method.  
        关键词：traditional Chinese medicine;Semi-bionic Extraction;microwave method;ultrasonic method;enzymic method   
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      • MA Chuan-jiang,LU Shuang,CAO Guang-shang,YANG Pei-min
        Vol. 22, Issue 18, Pages: 192-196(2016) DOI: 10.13422/j.cnki.syfjx.2016180192
        摘要：Paris polyphylla var. chinensis, as an important source of Paridis Rhizoma in Chinese Pharmacopoeia, has many pharmacological efficacies, such as clearing heat and toxic matter, relieving swelling and pain, cooling the liver and relieving convulsion, and been clinically used for anti-tumor, antibiosis, hemostasis, analgesia and so on. P. polyphylla var. chinensis, as a perennial plant, has a low reproductive rate and long growth period, with seven years from seed germination to medical application. Recently, its wild plant resources became rare because of the excessive excavation, which cannot meet the need of the market, caused a high market price, and even posed a serious threat to the survival and sustainable use of its resources. To solve this problem, developing artificial breeding technology and breeding the cultivated species are the best way to protect the wild plant resources and meet the clinical demands. Literature related to the breeding technology of P. polyphylla var. chinensis were retrieved and summarized to make a review about seed collection and seedling, so as to provide the theoretical guidance for the artificial breeding. Meanwhile, the breeding quality was discussed based on the correlative chemical composition during the growth of the seedlings. The breeding technology of P. polyphylla var. chinensis is the first important step to breed good-quality varieties, while the detection for the subsequent quality is significant as well. In this article, the quality evaluation system was established for wild and bred varieties in a multi-disciplinary and multi-angle manner, in order to guarantee the quality of Chinese medicinal materials, and provide a reference for further exploitation and utilization of P. polyphylla var. chinensis.  
        关键词：Paris polyphylla var. chinensis;breeding technology;chemical composition;advance in study   
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        发布时间：2024-01-04
      • ZHAO Lei,LIANG Mao-xin
        Vol. 22, Issue 18, Pages: 197-202(2016) DOI: 10.13422/j.cnki.syfjx.2016180197
        摘要：Objective: To find and demonstrate the potential functions of Salviae Miltiorrhizae Radix et Rhizoma. Method: The descriptions of Salviae Miltiorrhizae Radix et Rhizoma functions from old herbalism documents were investigated by comprehensive summary. Meanwhile, Pu Ji Fang database management system was used to retrieve the distribution and compositions of ancient compounds including Salviae Miltiorrhizae Radix et Rhizoma and summarize the overall functions. The results were further compared with Chinese Pharmacopeia to find the similarities and difficulties of its ancient and present functions, and explore its potential functions. This could be verified by modern pharmacological study and clinical application. Result: The functions of Salviae Miltiorrhizae Radix et Rhizoma from old herbalism documents could be summarized into 13 categories: promoting blood circulation to remove blood stasis, reinforcing Qi and nourishing blood, soothing nerves, cooling blood to remove carbuncle, clearing heat-fire, harmonizing blood to regulate menstruation, preventing miscarriage, dispelling wind and eliminating dampness, relieving pain, regulating the flow of Qi, suppressing hyperactive liver for calming endogenous wind, relieving itching and strengthening bone. The indications distribution and composition results ancient traditional Chinese medicine(TCM) compound prescriptions showed that Salviae Miltiorrhizae Radix et Rhizoma was widely used to treat 29 kinds of diseases, such as stroke, malady, skim and external diseases, serophthisis perniciosa endemica, asthenic diseases, and so on. Besides the functions and indications recorded by Chinese Pharmacopeia, Salviae Miltiorrhizae Radix et Rhizoma could be also used in treating wind-stroke syndrome, malady, atrophic debility of bones, edema, heat syndromes, cough, threatened abortion and other diseases. Modern pharmacological study and clinical application showed that Salviae Miltiorrhizae Radix et Rhizoma can protect brain tissues, regulate neurotransmitter disorder, promote osteoblast division and proliferation, bacteriostat, et al. Conclusion: After comparison between ancient documents and modern documents, combined with modern pharmacological study and clinical application, it was proved that Salviae Miltiorrhizae Radix et Rhizoma has potential functions of calming liver wind, smoothing spasm, guiding water to alleviate edema, strengthening bones, removing wind-damp syndromes, stopping cough, clearing heat-fire(clearing heat-toxicant), and preventing miscarriage.  
        关键词：Salviae Miltiorrhizae Radix et Rhizoma;herbalism;database management system of Pu Ji Fang;potential function   
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