最新刊期
卷 22 ， 期 19 ， 2016
- 摘要：Objective: To obtain sustained-release preparations of ferulic acid liposomes by optimizing its formulation and technology. Method: The content of ferulic acid in liposomes was determined by HPLC.Ferulic acid was encapsulated into liposomes by calcium acetate gradient method,dialysis method was used to investigate release of ferulic acid.The factors such as phospholipids,the content of cholesterol,particle size and the addition of blood components were intensively investigated.Moreover,thermosensitive hydrogel technique was further applied to obtain the satisfactory sustained-release effect. Result: Hydrogenated soybean phosphatidylcholine (HSPC) was selected as the phospholipid composition. Optimum molar ratio between HSPC and cholesterol was determined at 4:1.Optimum average size of liposomes was found to be 397.7 nm.Blood components could accelerate the release of ferulic acid liposomes and there was little difference among different blood (human serum,fetal calf serum and rat plasma).Moreover,with the application of thermosensitive hydrogel technique,sustained-release effect was obtained in 48 h and the kinetics was fitted with Higuchi equation. Conclusion: Fetal calf serum and rat plasma can be used in drug release studies of liposomes to mimic human serum.Optimization of formulation and technique factors as well as the combined application of thermosensitive hydrogel technique can result in the satisfactory sustained-release properties of ferulic acid liposomes.关键词：ferulic acid;liposomes;in vitro release;thermosensitive hydrogel;cholesterol
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of casticin chitosan microspheres and investigate its in vitro release characteristics. Method: Casticin chitosan microspheres were achieved by emulsion crosslinking method,taking liquid paraffin as oil phase,span-80 and tween-80 as emulsifier,and glutaraldehyde as crossinking agent.Design-Expert 8.0.6 software was applied to optimize preparation procedure.Morphology of the microspheres was observed by scanning electron microscope (SEM).Dynamic dialysis method was used to determine in vitro release characteristic of casticin chitosan microspheres. Result: Optimum preparation process of microspheres was as following:ratio of oil phase to water phase of 6:1,crosslinking time of 3 h,rotation rate of 1 400 r·min-1.Casticin chitosan microspheres had rounded shape and uniform particle size distribution,its average diameter was 7.92 μm,drug-loading was 29.20%and encapsulation efficiency was 39.23%.Release behavior of casticin chitosan microspheres was described by Higuchi model in phosphate buffer solution with pH of 7.4.Cumulative release amounts of casticin chitosan microspheres in 4 h and 48 h were 26.75%and 95%,respectively. Conclusion: This optimized preparation process is feasible and simple,casticin chitosan microspheres has high drug-loading with a sustained-release effect.关键词：casticin;chitosan;microspheres;drug-loading;emulsion crosslinking method;central composite design-response surface methodology
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Chaishao Changning granules. Method: Orthogonal design were used to optimize extraction process of Chaishao Changning granules by taking solvent volume,extraction time and times as factors,composite score among yield of dry extract,contents of paeoniflorin,berberine hrdrochloride and citric acid,intestinal propulsive rate and diarrhea index was index. Result: Optimum extraction process of Mume Fructus was:extracted twice with 10 times the amounts of water,1 h for per time;extracting amount of citric acid was 154.17 mg·g-1,yield of dry extract was 31.27%.Optimum extraction process of others herbs in Chaishao Changning granules was:extracted thrice with 10 times the amount of water,1 h for each time;extracting amounts of paeoniflorin and berberine hydrochloride were 3.97,0.66 mg·g-1,yield of dry extract was 26.36%,intestinal propulsive rate was 61.97%and diarrhea index was 0.98. Conclusion: These optimized processes are stable and feasible,which can provide reliable reference value for the actual production of Chaishao Changning granules.关键词：Chaishao Changning granules;yield of dry extract;paeoniflorin;berberine hydrochloride;citric acid;intestinal propulsive rate;diarrhea index
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Astragali Radix with honey bran. Method: Taking overall desirability of contents of astragaloside Ⅳ,calycosin glucoside,total flavonoids and water soluble extract as index,the amount of refine honey,frying time and temperature as factors,central composite design-response surface methodology was adopted to optimize processing technology. Result: Optimum processing technology was as following:refine honey amount of 9.6 g for 40 g of Astragali Radix,frying time of 4 min,frying temperature of 210℃.Under these conditions,contents of astragaloside Ⅳ,calycosin glucoside,total flavonoids and water soluble extract were 0.058%,0.035%,3.28%and 48.2%,respectively. Conclusion: This optimized processing technology of Astragali Radix is simple and feasible with good predictability.关键词：honey bran;Astragali Radix;astragaloside Ⅳ;calycosin glucoside;processing technology;total flavonoids;water soluble extract
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To explore effects of Atractylodis Rhizoma before and after being deep-fried on contents of aquaporin 2 (AQP2) and AQP3 in model rats with syndrome of damp retention in middle-jiao and provide an reference for mechanism research of Atractylodis Rhizoma before and after being deep-fried. Method: Animals were divided into the blank group,the damp retention in middle-jiao model group,the high,middle and low dose (3 459.2,1 729.6,864.8 mg·kg-1) group of raw products of Atractylodis Rhizoma,the high,middle and low dose (3 459.2,1 729.6,864.8 mg·kg-1) group of deep-fried products of Atractylodis Rhizoma,Xiangsha Weiling pills positive group (1 729.6 mg·kg-1),and rats were intragastric administration for seven days.Contents of AQP2 in urine and AQP3 in colonic mucosa were detected by UV and euzymelinked immunosorbent assay respectively before and after administration. Result: Compared with the blank group,AQP2 content increased and AQP3 content lowered in the model group; difference between these two groups were statistically significant.Compared with the model group,AQP2 content in administration group were declined and this index in raw products was lower than those of deep-fried group;the content of AQP3 in colonic mucosa rose dramatically and the deep-fried group was higher than that of raw product group. Conclusion: Both of raw and deep-fried products have effects of clearing damp and antidiarrheal.Function of raw products is stronger than deep-fried products in terms of clearing damp effect.However,in the aspect of antidiarrhoea,the role of deep-fried products is stronger than raw products.关键词：Atractylodis Rhizoma;deep-fried;aquaporin;damp retention in middle-jiao;Xiangsha Weiling pills
- 2
- |
- 2
- |
- 11
发布时间：2024-01-04 - 摘要：Objective: To compare specific HPLC fingerprints of Atractylodis Rhizoma before and after being processed with bran,and clear differences among characteristic peaks. Method: n-Hexane extracts of ten batches of raw products and the corresponding processed products with bran were analyzed by HPLC,mobile phase was acetonitrile-water for gradient elution,the detection wavelength was 254 nm.Taking atractylodin as the reference peak,common pattern of HPLC fingerprints about raw and processed products was constructed. Result: There were 26 common peaks in HPLC of raw and processed products of Atractylodis Rhizoma,the relative retention time of common peaks was almost the same with RSD <0.5%;but difference of their relative peak area was obvious.In HPLC fingerprints,the area ratio of chromatographic peaks at 47.36 min to 46.35 min was greater than 1 in raw products,but it was less than 1 in processed products;at the same time,the area ratio of peaks at 51.70 min to 49.25 min was less than 1 in raw products,but it was greater than 1 in processed products. Conclusion: Difference of chromatographic peaks in raw and processed products of Atractylodis Rhizoma is significant,and the results are stable with good specificity.It can be used as qualitative identification between raw and processed products of Atractylodis Rhizoma.关键词：Atractylodis Rhizoma;processed products with bran;fingerprints;n-hexane;atractylodin;atractylenolide Ⅱ;diisobutyl phthalate
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of volatile oil from Aurantii Fructus,and analyze difference of chemical constituents in volatile oil from Aurantii Fructus with grinding degree of 24 mesh and 50 mesh. Method: Volatile oil from Aurantii Fructus was extracted by steam distillation,orthogonal test was adopted to optimize process of volatile oil.GC-MS was employed to compare similarities and differences between volatile oil from Aurantii Fructus with grinding degree of 24 mesh and 50 mesh. Result: Optimum extraction conditions of steam distillation were as follows:the comminution degree of 24 mesh,soaked 0.5 h and extracted 7 h with 11 times the amount of water.Forty-five components in volatile oil from Aurantii Fructus with grinding degree of 24 mesh were identified,there were 39 components for 50 mesh,accounting for 99.46%and 99.53%of total content of volatile oil,respectively. Conclusion: This optimized process is stable and feasible with high extraction rate of volatile oil.关键词：Aurantii Fructus;volatile oil;extraction technology;steam distillation;grinding degree;D-limonene
- 2
- |
- 4
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To define the type of fungi causing Gardenia jasminoides pathogenic anthracnose, and study the inhibitory effect of commonly antisepticson the mycelial growth of the pathogens, in order to provide a basis for preventing and treating the disease. Method: G.jasminoides anthracnose leaves were collected from several regions of Jiangxi province. The pathogens were isolated and purified by general tissue separation. According to the Koch's postulates, pathogenicity test, pathogen morphological observation, microscopic identification and rDNA-ITS sequence analysis were used to identify the pathogens. Eleven fungicides were selected to test their indoor sterilizing effect. Thetoxicity regression equationfor agent concentration and inhibition ratio was established, and the effective fungicides were screened out, in a bid to provide a theoretical basis for controlling the disease. Result: Eleven strains were separated from G.jasminoides anthracnose leaves. Through pathogenic determination and Koch's Postulates, one strain was confirmed to be the main pathogen that attacks G.jasminoides quickly and severely. On the basis of morphology and microscopic identification, and Blast comparison analysis for rDNA-ITS sequences, the pathogenic strain was identified to be Phoma.sp. The fungicides Wuyiencin, streptomycin and oligosaccharide chain protein had no significant inhibition effect,but carbendazol showed the best sterilizing effect,whose regression equation was Y=79.97X-1.75 (r=0.965, EC50=0.65 mg·L-1). The next were prochloraz tricyclazole and pentanitro-carbendazim, whose regression equations were Y=6.142X+19.47(r=0.902, EC50=4.97 mg·L-1) and Y=6.1X+17.23(r=0.93, EC50=5.37 mg·L-1), respectively. The others showed varying inhibition effect. Conclusion: The G. jasminoides anthrax pathogen was determined, and effective agents to inhibit pathogens were screened out.关键词：Gardeniae Fructus;anthrax;pathogen;antimicrobial agent
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To clarify the antioxidative effects in vitro of Ginseng Radix et Rhizoma, Ophiopogonis Radix and Schisandrae Chinensis Fructus from Shengmai San. Method: With the use of superoxide anion (O2-·) H2O2 and ONOO- generation system, reactive oxygen species (ROS) scavenging effect of Ginseng Radix et Rhizoma, Ophiopogonis Radix and Schisandrae Chinensis Fructus was analyzed in offline mode; furthermore, three online ROS systems including HPLC-luminol-H2O2-CL, HPLC-luminol-pyrogallol-CL, HPLC-ONOO--luminol-CL were used to analyze the direct scavenging effect of Ginseng Radix et Rhizoma, Ophiopogonis Radix and Schisandrae Chinensis Fructus on O2-·, H2O2 and ONOO-, and clarify its effective components and antioxidative characteristics. Result: Water-soluble components could be the main constituents in scavenging three ROS, and lipophilic component exerted little effect on scavenging ROS. Conclusion: Three constituents with direct ROS scavenging effect were clarified with use of 3 ROS online scavenging systems, and the effect of directly scavenging ROS could be not the main effect of Shengmai San, providing basis and new thoughts for further study on mechanism of Shengmai San in preventing and treating myocardial ischemia injury.关键词：Ginseng Radix et Rhizoma;Ophiopogonis Radix;Schisandrae Chinensis Fructus;reactive oxygen species
- 2
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To study the HPLC fingerprint of the Jiuwei Xifeng granules, and establish a method for the content determination of rhyncholphylline, isorhynchophylline, corynoxeine and isocorynoxeine. Method: Phenomenex Gemini C18 column was used with methyl alcohol-0.1%triethylamine as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1; the column temperature was 35℃, and the detection wavelength was 246 nm. Ten batches of Jiuwei Xifeng granules were analyzed by HPLC fingerprints and content determination methods. Data were analyzed by using Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A). Result: In the fingerprints of well separated and repeated Jiuwei Xifeng granules, 13 common peaks were identified. The similarity among different batches was more than 0.96.Based on the comparison with reference products, four components, rhyncholphylline, isorhynchophylline, corynoxeine and isocorynoxeine were identified; quantitative analysis was conducted for the above components. Conclusion: The method is rapid, simple and accurate, it can be used for the quality control of Jiuwei Xifeng granules.关键词：Jiuwei Xifeng granules;fingerprint;rhyncholphylline;isorhynchophylline;corynoxeine;isocorynoxeine;HPLC
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To evaluate the anti-platelet aggregation effect of horminone and 7-O-acetylhorminone in roots of Salvia deserta, and to establish a method for simultaneous determination of three effective constituents(horminone, 7-O-acetylhorminone and 6,7-dehydroroyleanone) in S. deserta roots. Method: In vitro anti-platelet aggregation of horminone and 7-O-acetylhorminone was determined by turbidimetry. The contents of horminone, 7-O-acetylhorminone and 6,7-dehydroroyleanone were simultaneously determined by HPLC. Result: As compared with the normal saline group, horminone and 7-O-acetylhorminone of the high and middle dose groups could effectively inhibit platelet aggregation induced by thrombin in a clear dose-dependent manner. The horminone, 7-O-acetylhorminone and 6,7-dehydroroyleanone showed good linear relationship with the peak area within 0.31-4.56 μg(r=0.999 6), 0.01-0.24 μg(r=0.999 4) and 0.4-6.0 μg(r=0.999 5); their average recoveries were 100.3%(RSD 2.0%), 100.6%(RSD 3.1%), and 102.5%(RSD 1.6%) respectively. Conclusion: Horminone and 7-O-acetylhorminone in roots of S. deserta showed better anti-platelet aggregation effect. The method of HPLC is simple, accurate and repeatable foe content determination, and can be used for quality control and evaluation of S. deserta.关键词：Salvia deserta;HPLC;horminone;6,7-dehydroroyleanone
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of pharmaceutical technology and the quality of raw material on trace elements contents in Zhenzhu Mingmu eye drops, and provide information for the quality control and quality safety of this preparation. Method: The contents of Pb, Cd, As, Hg, Cu, Mg, Al, K, Ca, Cr, Mn, Zn, Sr, Pd, Ba, W in 102 batches of Zhenzhu Mingmu eye drops from 7 manufacturers were determined by inductively coupled plasma mass spectrometry (ICP-MS) method. Result: Various elements showed good linear relationship (r>0.999), and the recovery rate was within 78.6%-97.6%, RSD within 2.5%-9.5%, indicating the quantitative method was accurate and reliable. Conclusion: The results showed that the contents of As and Hg in some samples exceeded the recommended limits; the content of trace elements in products of few enterprises were quite different among the samples from other manufacturers, and the obvious fluctuation was even existed for the products from the same manufacturer.关键词：inductively coupled plasma mass spectrometry (ICP-MS);pearl powder;Zhenzhu Mingmu eye drops;trace elements
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To determine the contents of liquiritin, licorice acid and moisture in Glycyrrhizae Radix et Rhizoma by using near-infrared (NIR) quantification method. Method: Near-infrared diffused reflected spectroscopy was applied to obtain the NIR spectra of Glycyrrhizae Radix et Rhizoma and the content values were analyzed by using TQ Analyst software. The NIR method was established with partial least squares (PLS). Result: The root-mean-square error of prediction (RMSEP) value of licorice glycosides was 0.165, with correlation coefficient Rp value 0.986 8; root-mean-squares error of cross-validation (RMSECV) value was 0.393, with correlation coefficient Rv value 0.904 6.The RMSEP value of glycyrrhizic acid was 0.166, with correlation coefficient Rp value 0.995 5; the RMSECV value was 0.575, with correlation coefficient Rv value 0.954 0.The RMSEP value of moisture was 0.137, with correlation coefficient Rp value 0.995 2; RMSECV value was 0.498, with correlation coefficient Rv 0.931 9. Conclusion: The established method is accurate, rapid and convenient, which could be used for the content determination of liquiritin, licorice acid and moisture in Glycyrrhizae Radix.
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish UPLC method for the determination of DAW22 from Ferula ferulaeoides of different growth periods in Xinjiang. Method: Solid phase extraction(SPE) was used for purification, and the determination was performed on ACQUITY UPLC BEH C18(2.1 mm×50 mm, 1.7 μm) with methanol-1% formic acid solution as the mobile phase for gradient elution. The flow rate was 0.2 mL·min-1. Column temperature was 30℃ and UV detection wavelength was 316 nm. Result: The sample volume showed good linear relationship with area under the peak curve within the range of 6.21-124.2 ng(r=1.000 0) for DAW22. The average recovery was 99.81%, and the RSD was 2.0%. The content of DAW22 was highest for those grown in May 9th. Conclusion: This method is accurate, simple and reproducible, and can be applied to the quality control of F. ferulaeoides.关键词：Ferula ferulaeoides;DAW22;UPLC;content determination
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for simultaneous determination of 9 illegally added anti-diabetic chemical components in Chinese patent medicines. Method: The compounds were separated on C18 column, with methanol-0.1 mol·L-1 ammonium acetate solution as the mobile phase for gradient elution. The detection wavelength was set at 235 nm and the column temperature was 35℃, with the flow rate of 1.0 mol·L-1. Full wavelength scanning was from 190 nm to 400 nm. Suspicious ingredients were confirmed by mass spectrometry. Result: All 9 components had good linear relationship within their respective concentration range(r≥0.999 9), and their precision, stability, limit of detection, limit of quantitation and recovery rate met the standards. Illegally added glyburide was detected in Jiangtangning. Conclusion: The method is simple, accurate, and suitable for the determination of 9 illegally added anti-diabetic chemical components above.关键词：Chinese patent medicines;illegally added;anti-diabetic chemical components;HPLC-MS
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate effect of Glycyrrhizae Radix et Rhizoma on metabonomics of triptonide,find toxicity biomarkers and attenuation biomarkers induced by Glycyrrhizae Radix et Rhizoma and disclose toxicity and attenuation mechanism of Glycyrrhizae Radix et Rhizoma on triptonide. Method: SD rats were randomly divided into three groups of the control group,the triptonide group (dose of 0.7 mg·kg-1) and the triptonide (dose of 0.7 mg·kg-1)+Glycyrrhizae Radix et Rhizoma (dose of 3 mL·kg-1) group.All drug treatments were administered daily for consecutive 7 days.All urine samples were determined by UPLC-Q-TOF-MS.These obtained data were analyzed by principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA). Result: Seven biomarkers related to toxicity of triptonide were found,four of them were identified as pantothenic acid,kynurenic acid,tryptophan and coproporphyrin,respectively.Four attenuation biomarkers were identified,one of them was tryptophan,but these remaining three biomarkers were unknown. Conclusion: These toxicity biomarkers and attenuation biomarkers are identified for the first time.It is indicated that toxicity of triptonide interfered with the energy,tryptophan and porphyrin metabolisms in body,recover of these metabolisms induced by Glycyrrhizae Radix et Rhizoma may be a physiological attenuation mechanism of Glycyrrhizae Radix et Rhizoma on triptonide.关键词：Glycyrrhizae Radix et Rhizoma;triptonide;metabonomics;attenuation biomarker;tryptophan;coproporphyrin;pantothenic acid
- 2
- |
- 2
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects of oxymatrine (OMT) on rat primary cardiomyocyte injury induced by aldosterone (ALD), and explore its effects on transcription and expression of mineralcorticoid receptor (MR). Method: Primary cardiomyocytes of Sprague-Dawley (SD) rats were isolated by trypsin digestion method and purified by differential adhesion method. The cardiomycoytes and their purity were identified by troponin and actin immunocytochemical staining, and ALD-induced cell injury models were established. The optimal ALD concentration and treatment duration were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The experiment was divided into 4 groups:blank group (serum free cell culture), ALD group (optimal concentration), OMT high dose group (ALD+100 mg·L-1 OMT) and OMT low dose group (ALD+50 mg·L-1 OMT). Preincubated with OMT 2 h, and then ALD was added for co-culture for 36 h. The protection effects of OMT on ALD-induced injury were detected and analyzed by MTT assay and lactate dehydrogenase (LDH) leakage assay. Reverse transcription PCR (RT-PCR) was used to analyze the mRNA of mineralocorticoid receptor (MR). The protein expression levels of MR and CYP11B2 were detected by Western blot. Result: The 16 mg·L-1 ALD significantly induced cell injury after 36 h. ALD-induced cardiomyocyte viability decrease and LDH leakage increase were significantly attenuated by OMT (100, 50 mg·L-1). Furthermore, OMT (100,50 mg·L-1) suppressed ALD-induced up-regulation of MR mRNA levels and protein expression levels, and enhanced protein expression of CYP11B2. Conclusion: OMT had protection effects on ALD-induced cardiomyocyte injury, and the mechanism may be associated with inhibiting ALD/MR signaling activation.关键词：oxymatrine;aldosterone;mineralocorticoid receptor;cardiomyocyte;CYP11B2
- 2
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To observe the anti-hepatitis B virus (HBV) efficacy and the action mechanism of HH capsule. Method: Dalian Elite ODS-BP C18 column was used to study the HH capsule HPLC fingerprint. HBV transgenic mice were randomly divided into four groups,such as matrine group, high and low dose HH capsule groups, and model group. The mice in matrine group received the same volume of suspension containing 150 mg·kg-1·d-1 matrine; mice in high and low dose HH capsule groups received the same volume of suspension containing 7 g·kg-1·d-1 and 2 g·kg-1·d-1HH capsule; the mice in model group received the same volume of normal saline. After treatment for five weeks, ELISA method was used to detect hepatitis B virus surface antigen (HBsAg), IFNα and IFNβ in the serum as well as HBsAg in liver tissues. Conventional HE staining was used to observe pathological changes in liver tissues, and quantitative PCR was used to detect hepatitis B virus DNA (HBVDNA) in serum and liver tissues. Result: The 14 characteristic peaks were identified in HH capsules fingerprint, including the 1st peak of gallic acid, the 8th peak of corilagin, the 9th peak of polydatin, the 10th peak of ellagic acid, the 13th peak of glycyrrhizin, and the 14th peak of oleanolic acid. As compared with the model group, high dose (7 g·kg-1·d-1) HH capsule can significantly reduce HBsAg and HBVDNA levels in the serum and liver of HBV transgenic mice, with statistically significant difference(P<0.01), but the difference was not statistically significant as compared with matrine group. High-dose HH capsule could significantly increase the levels of IFN-α and IFN-β in the mice with statistically significant difference(P<0.01, P<0.05). Conclusion: HH capsule has reliable anti-HBV effect, which may be associated with increasing IFN-α and IFN-β levels.关键词：HH capsule;hepatitis B virus transgenic mice;anti-HBV;interferon-α/β.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect of nitidine chloride (NC) on expression of DNA polymerase δ catalytic subunit P125 in breast cancer cells (MCF-7 cells), and investigate the mechanism of NC on inhibiting proliferation of breast cancer cells. Method: Different concentrations (0.075, 0.15, 0.3, 0.60, 1.20, 4.80 mg·L-1) NC groups and blank group were established. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was applied to evaluate the inhibitory rate of cells after treatment with different concentrations of NC. At the same time, the clone form assay was used to further verify the inhibitory effect of different concentrations of NC on MCF-7 cells proliferation, the effects of different concentrations of NC (0.50, 1.00, 2.00 mg·L-1) on mRNA expressions of DNA polymerase δ catalytic subunit gene POLD1, p53 and p21 were quantified by Real-time PCR. The protein expression of P125 was detected by Western blot, and the protein expressions of p53 and p21 were detected by enzyme linked immunosorbent assay (ELISA). Result: NC had obvious inhibitory effect on MCF-7 cells in a concentration-dependent manner. NC with concentrations of 0.075, 0.15, 0.3, 0.60, 1.20, 2.40, 4.80 mg·L-1 had an inhibitory rate of 0.16%, 6.29%, 19.56%, 32.17%, 44.46%, 73.46%and 83.21%respectively on the cells. As compared with the blank group, NC had significant inhibitory effect on the formation of cell colonies (P<0.01), in addition, the mRNA expression of POLD1 and the protein expression of P125 in various NC groups were significantly decreased (P<0.05), the mRNA and protein expressions of p53 were significantly up-regulated (P<0.01), but the mRNA and protein expressions of p21 were significantly up-regulated only in the high dose NC group (P<0.01). Conclusion: NC can significantly inhibit the proliferation of MCF-7, and one of the mechanisms may be associated with up-regulating the level of p53 expression and inhibiting the expression of P125.关键词：nitidine chloride;MCF-7;P125;DNA polymerase delta catalytic subunit gene 1 (POLD1);p53;p21
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Biejiajian Wan (BP) on angiogenesisand the structure of the new vessels in Matrigel implant of mice induced by basic fibroblast growth factor(b-FGF). Method: Totally 32 Balb/c mice were selected, and angiogenesis model was established by matrigel implant assay. Matrigel (500 μL) containing b-FGF and heparin was subcutaneously transplanted into the abdomens of mice, and then the mice were randomly divided into BP high dose and low dose groups (12, 6 mg·kg-1), normal group, endostatin group (5 mg·kg-1), n=8 in each group. The corresponding drugs were given 3 days before Matrigel implant for 8 days. Matrigel was taken out at the eighth day, and hemoglobin content in Matrigel implant was detected; HE staining and immunohistochemical staining were used to detect microvessel density count (MDC); meanwhile, the structure characteristics and development stage of the new vessels in matrigel implant were observed and compared. Result: Hemoglobin content and MDC in high and low dose BP groups were lower than those in normal group; in addition, the hemoglobin content and MDC in low dose BP group were lower than those in high dose group. HE staining results showed some scattered new vessels in low dose and high dose BP groups, and the number of new vessels was lower than that in normal group. Furthermore, CD31 immunohistochemical staining results showed that the number of endothelial cells in BP groups was lower than that in normal group; the structure of the new vessels became fuzzy and the endothelial cells became lighter in color as compared with the control group. Conclusion: BP have inhibitory effect on angiogenesis in a concentration-dependent manner; low dose BP has a stronger inhibitory effect than high dose BP on angiogenesis.关键词：Biejiajian Wan;Matrigel;angiogenesis;vascular normalization
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of essential oil from Aucklandiae Radix on proliferation and apoptosis inhibition of MV4-11 leukemia cells and discuss its action mechanism. Method: Human leukemia cell line MV4-11 was treated with the essential oil from Aucklandiae Radix (3,6,12,25,50,100 mg·L-1) for different time, and another blank group was set up. Cell viability was estimated by using MTT assay; the apoptosis morphology changes were observed by Hoechst 33258 staining; flow cytometry was used on Annexin V-FITC/propidium iodide staining to detect the cell cycle and the rate of apoptosis; Western blot was used to detect AKT and Caspase-3 protein expression. Result: The essential oil from Aucklandiae Radix had IC50 value of 13.33 mg·L-1; various nuclear changes such as nuclear shrinkage, chromatin condensation and obvious apoptotic bodies were observed after MV4-11 cell line was treated with essential oil from Aucklandiae Radix for 24 h. As compared with the blank group, the cells in G0/G1 phase were significantly reduced and the cells in S phase were significantly increased with the increase from essential oil from Aucklandiae Radix oil dosage (P<0.05, P<0.01). 12, 25, 50, 100, 150 mg·L-1 essential oil from Aucklandiae Radix induced apoptosis of MV4-11 (P<0.05); 25, 50, 100, 150 mg·L-1 essential oil from Aucklandiae Radix inhibited AKT phosphorylation and promoted Caspase-3 protein degradation (P<0.05, P<0.01). Conclusion: The essential oil from Aucklandiae Radix can inhibit the proliferation of MV4-11 cells in vitro, and the mechanism may be correlated with inducing cell apoptosis by inhibiting AKT activity.关键词：essential oil from Aucklandiae Radix;apoptosis;MV4-11 leukemia cells;proliferation;protein kinase B;Caspase-3
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Bushen Tiaochong prescription on protein and mRNA expression levels of sex hormone, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in ovaries of rats with premature ovarian failure(POF) induced by triptergium wilfordii (GTW), and explore the prevention effect of Bushen Tiaochong prescription. Method: Forty-two healthy female SD rats were randomly divided into normal group, model group, estrogen group, Bushen Tiaochong prescription low, middle and high dose groups. 17-estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and progesterone (P) levels in serum were detected by enzyme-linked immunosorbent assay (ELISA). Expression levels of Bcl-2 and Bax protein and mRNA in rats ovarian were detected by Western blot and RT-PCR methods. Result: As compared with the normal group, FSH and LH contents were significantly increased in model group rats; E2 contents were significantly decreased; Bcl-2 protein and mRNA expression levels in ovarian were significantly decreased; while Bax protein and mRNA expression levels were significantly increased (P<0.01). E2 content in Bushen Tiaochong prescription groups and estrogen group was higher than that in model group (P<0.01), but middle, high dose groups and estrogen group had no statistically significant differences in E2 as compared with the normal group. The FSH and LH contents in Bushen Tiaochong prescription groups and estrogen group were lower than those in model group(P<0.01), and there were no statistically significant differences as compared with normal group. All dose groups had no significant difference with normal group in P content. Bcl-2 protein and mRNA expression levels in middle and high dose Bushen Tiaochong prescription groups and estrogen group were significantly higher than those in model group (P<0.01); Bax protein and mRNA expression levels were significantly lower than those in model group(P<0.01). Conclusion: It is indicated that Bushen Tiaochong prescription has some protective effects for ovaries and prevents POF. It can regulate sex hormone level, maintain Bcl-2 and Bax balance to decrease follicles atresia and death speed, and improve ovarian function.关键词：Bushen Tiaochong prescription;premature ovarian failure;sex hormone;B-cell lymphoma-2;Bcl-2 associated X protein
- 2
- |
- 2
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To investigate the general toxicity and embryotoxicity of Shengbanxia decoction (BXd) as well as Ganjiang Renshen Banxia Tang (GJRSBXT), a famous prescription from Synopsis of Golden Chamber, and explore their action mechanism. Method: General acute toxicity testing and flow cytometry were applied to compare the effects of BXd and GJRSBXT in 4 times of ZHANG Zhong-jing's dosage on body weight, viscera pathology and T lymphocyte subsets of unpregnant mice. Reproductive toxicity test in sensitive period of teratogenesis was applied to compare BXd and GJRSBXT on gestation and embryonic development in mice. Result: As compared with the normal group, there was no obvious toxicity on body weight and pathology of main organs in unpregnant mice after treatment with BXd (2.275 g·kg-1) or GJRSBXT (5.688 g·kg-1) for 14 days, there was no distinct toxicity on heart, liver and kidney of maternal mice and fetal indicators after the pregnant mice in sensitive period of teratogenesis were treated with BXd or GJRSBXT for 10 days. However, GJRSBXT induced obvious decrease of CD4+T cell and significant raise of CD8+T cell lymphocyte proportion of blood and spleen in unpregnant mice after short-term repeated administration for 14 days (P<0.05). Conclusion: All the above results suggest that BXd and GJRSBXT do not have embryotoxicity in the dosage of this experiment, but may be still accompanied by immunosuppression effect. The causes and mechanisms need further research for clarification.关键词：Pinelliae Rhizoma;Ganjiang Renshen Banxia Tang;embryotoxicity;immunosuppression;T lymphocyte subsets
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Polygoni Cuspidati Rhizoma et Radix extract and its effective parts on gouty arthritis. Method: SD rats were randomly divided into 8 groups,such as the blank group,model group,colchicine group(0.28 mg·kg-1),high,middle and low(260,130,65 mg·kg-1) dose group of Polygoni Cuspidati Rhizoma et Radix extract,anthraquinone effective part group(11 mg·kg-1),stilbene effective part group(650 mg·kg-1),continuous intragastric administration of 14 days for once a day,after administration of 1 h in the twelfth day,rats were injected with 0.2 mL of uric acid sodium solution at the right hind limb,the right hind limb's circumference of rats were measured at 2,4,8,12,24,48 h in the same site,the degree of swelling was calculated.Colorimetric method was adopted to determine the level of uric acid(UA) and xanthine oxidase(XOD) in rat serum.Hematoxylin-eosin(HE) staining was employed to observe the pathological changes of the rat ankle joint synovial tissue. Result: Compared with the blank group,rats ankle swelling degree of the model group increased significantly.Compared with the model group,high and middle dose group of Polygoni Cuspidati Rhizoma et Radix extract and colchicine group can significantly reduce swelling degree,anthraquinone effective part group at 2,4 h can significantly reduce swelling degree,stilbene effective part group in 2,4,8,12,24,48 h can significantly reduce swelling degree.Compared with the blank group,UA and XOD levels in serum were significantly increased in the model group.Compared with the model group,Polygoni Cuspidati Rhizoma et Radix extract groups and its effective part groups can significantly reduce UA and XOD levels.Polygoni Cuspidati Rhizoma et Radix extract and its effective part can improve the pathology of synovial tissue. Conclusion: Anti-gouty arthritis of Polygoni Cuspidati Rhizoma et Radix extract may be the result of joint action of stilbene and anthraquinone.关键词：Polygoni Cuspidati Rhizoma et Radix;gouty arthritis;uric acid;xanthine oxidase;stilbene;anthraquinone
- 2
- |
- 2
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Ershiwuwei Songshi pills on liver injury in rabbits with endotoxemia, and observe the morphological changes in liver tissues of rabbits. Method: Thirty rabbits were randomly divided into five groups:model group, Ershiwuwei Songshi pills high dose, middle dose and low dose groups (0.5, 0.167, 0.06 g·kg-1, n=6), and dexamethasone group (0.005 g·kg-1), n=6 in each group. The rabbits were treated with corresponding drugs once a day for continuous 5 days by ig administration; the rats in model group and dexamethasone group received the same volume of normal saline by ig administration. Endotoxemia was used to attack the rabbits before drug administration on day 5, and the death of rabbits in each group was compared in 12 h; body temperature, blood routine and liver function were examined, and pathological changes of liver tissues were also observed by htoxylin eosin(HE) staining in each group. Result: the content of total bilirubin an alanine aminotransferase(ALT) in serum can be reduced by high dose Ershiwuwei Songshi pills and liver pathological damage was significantly reduced; the content of aspartate aminotransferase(AST) in serum and numbers of WBC can be reduced by low dose of Ershiwuwei Songshi pills, with significant difference as compared with the model group (P<0.05). Conclusion: Ershiwuwei Songshi pills has a protective effect on liver injury of rabbits induced by endotoxin.关键词：Ershiwuwei Songshi pills;liver injury with endotoxin;pathological score;routine blood test;liver function
- 2
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To explore the effects of polysaccharide from Lepidium meyenii raizomel(PLR) on the immune organs in subacute aging mice induced by D-galactose, and investigate its action mechanism. Method: Fifty ICR mice were randomly divided into five groups:normal group, D-galactose model group, and PLR low-, middle-, and high-dose groups. 500 mg·kg-1 of D-galactose was given to the mice by ih administration to produce aging models. The mice in PLR groups were given with PLR solution (75, 150, 300 mg·kg-1) by ig administration. The drugs were given for 56 days, and then the activities of superoxide dismutase(SOD), catalase(CAT), glutathion peroxidase(GSH-Px), monoamine oxidase(MAO) and the content of malondialdehyde(MDA) in mice serum or liver tissues were detected; the thymus ultrastructural changes were observed under TEM; the mRNA expression levels of SOD, CAT and p53 in the spleen were determined by qPCR. Result: As compared with the normal group, splenic sinusoid was extended in cells of model group, with large perinuclear space and chromatin margination; apoptosis was present in some cells; the SOD and CAT activities in serum of model group mice were significantly decreased; p53 mRNA expression in spleen tissues was significantly increased; SOD2 and CAT mRNA expressions were significantly decreased (P<0.05, P<0.01). As compared with the model group, distribution of lymphocytes in thymus was even in PLR middle dose and high dose groups; with clear perinuclear boundaries; no vacuoles in the cytoplasm; PLR could enhance the activities of SOD, CAT, GSH-Px, and reduce the content of MDA and activity of MAO. PLR high dose group significantly down-regulated the expression of p53 mRNA in spleen; middle dose and high dose groups significantly increased CAT mRNA expression; high dose group significantly increased SOD2 mRNA level (P<0.05, P<0.01). Conclusion: PLR has a protective effect on the immune organs of aging mice models, and the mechanism may be associated with increasing antioxidant enzyme activity and antioxidant enzyme mRNA expression, and decreasing aging-related enzyme activity.关键词：polysaccharide from Lepidium meyenii rhizome;subacute aging;anti-aging;immune organs;D-galactose
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of Xiaozheng Yin for the pain in endometriosis (EMS) with Qi stagnation and blood stasis and observe its effects on levels of matrix metalloproteinase (MMP)-3, MMP-9, vascular endothelial growth factor (VEGF) and tumor necrosis factor-α (TNF-α). Method: One hundred and eight patients were randomly divided into control group (53 cases) and observation group (55 cases). Patients in both groups took Gestrinone capsules, 2.5 mg/time, 2 times/week, continuing for six months. Patients in control group also took Sanjie Zhentong capsule, 4 pieces/time, 3 times/day, while patients in observation group orally took Xiaozheng Yin, 1 dose/day. Both groups took drugs 28 days a month, continuing for 6 months. Medicines were not withdrawn in menstrual cycle period. The levels of serum cancer antigen 125(CA125) and CA199, levels of serum MMP-3, MMP-9, VEGF and TNF-α were detected before and after treatment. Scores of pain, symptoms and endometriosis health profile-5 (EHP-5) were graded before and after treatment. Result: After therapy, scores of visual analogue scale (VAS), dysmenorrhea, pelvic pain, pelvic tenderness, and sacral ligament tenderness nodules in observation group were significantly lower than those in control group (P<0.01). After treatment, levels of CA125 and CA199 in observation group were lower than those in control group (P<0.05), levels of serum MMP-3, MMP-9, VEGF and TNF-α in observation group were less than those in control group (P<0.05, P<0.01), the scores of pain, emotional state, control, sexual life, infertility and treatment effects at EHP-5 in observation group were significantly lower than those in control group (P<0.01). The total effective rate in treatment group was superior to that in control group (P<0.05). Conclusion: Xiaozheng Yin can obviously improve pain for EMS patients, enhance their quality of life, reduce levels of serum CA125, CA199, MMP-3, MMP-9, VEGF and TNF-α. Therefore, it can promote disease recovery.关键词：endometriosis;pain;Xiaozheng Yin;endometriosis health profile-5;matrix metalloproteinases;vascular endothelial growth factor;tumor necrosis factor-α
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Daozhi Paishi decoction on the urine metabolism in patients with Qi stagnation, blood stasis and damp-heat pouring down type kidney stone. Method: The 180 patients with kidney stone accompanied with abnormal urine metabolism were randomly divided into treatment group and control group. According to the different types of abnormal urine metabolism, all the patients received corresponding life guidance and prevention and treatment suggestions. In treatment group, patients also received Daozhi Paishi decoction, 200 mL/time, bid, with 7 days as one treatment course, for a total of 4 courses. After that, the kidney stones were detected with B-ultrasound and X-ray, and the 24-hour urine biochemical detection was done with auto-biochemical analyzer. Result: After treatment, the number of cured cases and total effective rate in treatment group were higher than those in control group (P<0.05). The abnormal rate of urinemetabolism in both groups were was decreased after treatment (P<0.05). The rates of hypercalciuria, hyperoxaluria, high phosphorus urine and low urine output in treatment group were significantly lower than those in control group (P<0.05). The stone elimination rates in treatment group were higher than those in control group (P<0.05). The levels of 24 h urinary calcium, uric acid and oxalic acid in both groups were decreased after treatment (P<0.05), while the levels of 24 h urinary magnesium and urine volume in both groups were increased after treatment (P<0.05). The level of 24 h urinary phosphorus in treatment group was decreased while the level of 24 h urinary citrate in treatment group was increased after treatment (P<0.05). The levels of 24 h urinary calcium, uric acid, oxalic acid, and urinary phosphorus in treatment group were lower than those in control group (P<0.05), while the levels of 24 h urinary magnesium, citrate, and urine volume in treatment group were higher than those in control group (P<0.05). Conclusion: Daozhi Paishi decoction could accelerate the elimination of kidney stones and effectively intervene the urine metabolism of the kidney radiopaque calculus formers to prevent the development of the disease.关键词：Daozhi Paishi decoction;simple kidney radiopaque calculus;urinary metabolic abnormalities;24-hour urine biochemical detection
- 4
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To discuss the effects of Dizhuo Huatan decoction on lung function and inflammatory factors in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) with syndrome of phlegm and blood stasis obstructing lung, and investigate its action mechanism. Method: One hundred and fifty-six patients were randomly divided into control group and observation group. Patients in control group took controlled oxygen therapy, anti-infection, hormone, bronchodilators, ipratropium bromide aerosol, 4 times/day, 2 sprays/time, cefminox sodium for injection, 2 g/time, 2 times/day, acetylcysteine granules, 0.4 g/time, 3 times/day. Based on the treatment of control group, patients in observation group also received Dizhuo Huatan decoction, 1 dose/day. The treatment course was 2 weeks for both groups. Before and after treatment, lung function was detected, forced vital capacity rate of one second (FEV/FVC) and forced expiratory volume in 1 second (FEV1%) were recorded. Blood oxygen pressure (PaO2), partial pressure of carbon dioxide (PaCO2) and oxygen saturation were detected before and after treatment. Scores of syndrome of phlegm and blood stasis obstructing lung and difficulty in breathing severity were evaluated by Modified the medical research council respiratory questionnaire (mMRC) and COPD patients self assessment test (CAT) before and after treatment. The levels of nuclear factor-κB (NF-κB), serum interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and hypersensitive C-reactive protein (hs-CRP) were detected. Result: The total clinical effect rate in observation group was higher than that in control group (P<0.05). Pulmonary function grading of airflow limitation severity in observation group was lower than that in control group (P<0.05). Scores of syndrome of phlegm and blood stasis obstructing lung, mMRC and CAT in observation group were lower than those in control group (P<0.01). FEV1 and FEV1/FVC in observation group were higher than those in control group after treatment (P<0.01). PaCO2 in observation group was lower than that in control group, and levels of PaO2 and SaO2were higher than those in control group after treatment (P<0.01). Levels of NF-κB, TNF-α and hs-CRP in observation group were lower than those in control group, while the level of IL-10 was higher than that in control group after treatment (P<0.01). Conclusion: Based on the treatment of routine western medicine, Dizhuo Huatan decoction can ameliorate symptoms and lung function and relieve inflammatory response when it is used for AECOPD with syndrome of phlegm and blood stasis obstructing lung.关键词：chronic obstructive pulmonary disease;acute exacerbation;syndrome of phlegm and blood stasis obstructing lung;Dizhuo Huatan decoction;serum interleukin-10;tumor necrosis factor-α;nuclear factor-κB;hypersensitive c-reactive protein
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of Hushen Tonglin decoction on kidney injury and its effects on levels of superoxide dismutase in blood serum (SOD) and malonaldehyde (MDA) in patients with kidney calculi after extracorporeal shock-wave lithotripsy (ESWL). Method: One hundred and twenty-eight patients with kidney calculi were randomly divided into control group (63 cases) and observation group (65 cases). Patients of both groups received ESWL treatment, and symptomatic treatment such as anti-infection was given after surgery. Patients in control group received Paishi capsules for 14 days, 20 g/time, 3 times/day. Patients in observation group took Hushen Tonglin decoction for 14 days, 1 dose/day. Time of lumbago relief and disappearance time of gross hematuria were recorded. At the 1th, 7th and 14th day after operation, the removed calculi were detected by B-ultrasound. At the 12th hour, 7th and 14th day after operation, levels of SOD, MDA, β2-microglobulin (β2-MG), urinary N-acetyl-β-glucosaminidase (NAG) and cysteine protease inhibitors (CysC) were detected. Result: The effective rate was 93.85%in observation group, higher than 82.54%in control group (P<0.05). The time of lumbago relief and disappearance time of gross hematuria in observation group were shorter than those in control group (P<0.01). At the 7th and 14th day after operation, level of SOD in observation group was higher than that in control group (P<0.01), and the levels of MDA, NAG, β2-MG and CysC were lower than those in control group (P<0.01). Conclusion: Hushen Tonglin decoction can shorten the disease course, improve clinical efficacy, relieve complications with antioxidant effects, reduce renal parenchyma injury and promote the recovery of patients after ESWL in patients with kidney calculi.关键词：kidney calculi;extracorporeal shock-wave lithotripsy;Hushen Tonglin decoction;kidney injury;superoxide dismutase;Malonaldehyde
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of modified treatment of Zhenwu Tang combined with Xiaoyao San in treating chronic cardiac failure with depression, and investigate its effects on levels of angiotensin Ⅱ (AngⅡ), N-terminal pro brain natriuretic peptide (NT-proBNP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and serum hypersensitive C-reactive protein (hs-CRP). Method: One hundred and forty-eight patients were randomly divided into control group and observation group. Patients in control group took neurostan, 1 tablet/time, 3 times/day, while patients in observation group took modified treatment of Zhenwu Tang combined with Xiaoyao San, 1 dose/day. The treatment course was 12 weeks for both groups. Before and after treatment, scores of Hamilton's depression scale (HAMD), Hamilton anxiety scale (HAMA), Pittsburgh sleep quality index (PSQI) and Minnesota heart failure quality of life questionnaire (MLHFQ) were graded. Left ventricular end diastolic diameter (LVED), left ventricular systolic dimension (LVSD), left ventricular ejection fraction (LVEF) and cardiac output (CO) were detected by echocardiogram, and the score of Lee's heart failure was grated both before and after treatment. Levels of AngⅡ, NT-proBNP, IL-6, TNF-α and hs-CRP were detected before and after treatment. Result: The total effective rate for heart failure, the total effective rate for depression and clinical efficacy rate of traditional Chinese medicine(TCM) syndrome in observation group were higher than those in control group (P<0.05, P<0.01). Scores of HAMD, HAMA, PSQI and MLHFQ in observation group were lower than those in control group (P<0.01). Scores of LVED, LVSD and Lee in observation group were lower than those in control group after treatment, while the scores of LVEF and CO were higher than those in control group (P<0.01). The levels of AngⅡ, NT-proBNP, IL-6, TNF-α and hs-CRP in observation group were lower than those in control group after treatment (P<0.01). Conclusion: Zhenwu Tang combined with Xiaoyao San can ameliorate cardiac failure and depression symptoms, improve quality of life and regulate levels of AngⅡ,NT-proBNP medium and level of inflammatory mediator in treating chronic cardiac failure concomitant with depression, and the effect was obvious.关键词：chronic cardiac failure;depression;Zhenwu Tang;Xiaoyao San;angiotensin Ⅱ;N-terminal pro brain natriuretic peptide;inflammatory response
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of modified treatment of Shaoyao Tang retention enema combined with mesalazine in treating large intestine damp-heat type ulcerative colitis(UC), and investigate its effects on immuno-inflammatory responses. Method: One hundred and sixty UC patients were randomly divided into control group(80 cases) and observation group(80 cases) by random number table. Patients in control group took mesalazine enteric-coated tablets by swallowwing, 1 g/time, 4 times/day, hydrocortisone sodium succinate for enema, 100-200 mg. Patients in observation group took modified treatment of Shaoyao Tang retention enema combined with mesalazine. For both groups, enema was retained for 5 days a week and 2 days for rest, with continuous treatment courses of 8 weeks. Before and after treatment, scores of Sutherland disease activity index, Baron scores, Geboes indexes and large intestine damp-heat syndrome scores were graded. Patients' quality of life were evaluated by IBDQ scale. Levels of tumor necrosis factor-α(TNF-α), interleukin-4(IL-4), interleukin-1β(IL-1β), interleukin-10(IL-10), transforming growth factor(TGF-β), and matrix metalloproteinase-1(MMP-1) were detected before and after treatment. Result: The total effect rate of comprehensive efficacy was 90%in observation group, higher than 76.25%in control group, with statistically significant difference between two groups(P<0.05). The total effect rate of TCM syndrome was 92.5%in observation group, higher than 75%in control group, with statistically significant difference between two groups(P<0.01). After treatment, the scores of Sutherland disease activity index, Baron scores, Geboes indexes and large intestine damp-heat syndrome scores in observation group were all lower than those in control group(P<0.01). The individual scores and total scores of intestinal symptoms, general symptoms, emotional capability and social competence in IBDQ scale of observation group were higher than those in control group(P<0.01). Levels of serum IL-1β, TNF-α and MMP-1 were lower than those in control group, and levels of IL-4, IL-10 and TGF-β were higher than those in control group(P<0.01). Conclusion: Modified treatment of Shaoyao Tang retention enema combined with mesalazine can improve clinical symptoms,patients' quality of life and healing quality of intestinal mucosa, and regulate immuno-inflammatory responses in treatment of UC with large intestine damp-heat at active stage.关键词：ulcerative colitis;large intestine damp-heat;Shaoyao Tang;retention enema;immuno-inflammatory responses
- 2
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Tongluo Qingkong decoction in treating migraine within the acute attack period based on differentiation and its influence on serum levels of 5-hydroxytryptamine (5-HT), substance P (SP), endothelin (ET), homocysteine (Hcy) and nitric oxide (NO). Method: One hundred and twenty-one eligible patients were randomly divided into observation group (60 cases) and control group (61 cases) by number tablet. Control group took Changchun sustained release capsules and Ibuprofen tablets. In addition to the therapy of control group, patients in observation group were additionally administered with modified Tongluo Qingkong decoction based on differentiation. Courses of treatment in two groups continued for 4 weeks. Immediate analgesic effect was valuated, and migraine disability assessment questionnaire (MIDAS) was adopted to evaluate quality of life. Main symptoms, such as degree of headache, onset frequency, duration and symptoms, were scored. Levels of 5-HT, SP, ET, NO and Hcy were detected. Result: The effect rate of immediate curative effect in observation group was 83.33%, which was superior to 63.93%in control group (P<0.05). After treatment, the effect rate of short-term clinical effect in observation group was 93.33%, which was superior to 80.33%in control group (P<0.05). At the 2, 3, 5 and 7 h after treatment, score of VAS in observation group was lower than that in control group (P<0.01 or P<0.05). And after treatment, scores of MIDAS and main symptoms in observation group were lower than those in control group (P<0.01). Compared with the healthy control group, the lovels of 5-HT in the two groups before treatment were significantly decreased, and levels of SP, ET, NO and Hcy were higher than those in healthy control group (P<0.01). And level of 5-HT in observation group was higher than that in control group (P<0.01). Levels of SP, ET, NO and Hcy were lower than those in control group (P<0.01). Conclusion: In addition to the medicine western routine therapy, Tongluo Qingkong decoction based on differentiation can be adopted to relieve pain quickly, reduce frequency of pain attack, improve qualities of live within the acute attack period, with superior immediate curative effect and short-term effect to the pure western medicine therapy. Its action mechanism may be correlated with the regulation of nerve-endocrine factors and vascular endothelial functions.关键词：migraine;Tongluo Qingkong decoction;5-hydroxytryptamine;substance P;endothelin;homocysteine;nitric oxide
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To search small molecule inhibitors for HIV-1 intergrase from traditional Chinese medicine database platform (TCMSP) by using the virtual screening technology. Method: The interacting site between HIV-1 intergrase andcytokineLEDGF/P75 were taken as target. The molecular docking technology was used for the first round of screening, then the ADME/T prediction was adopted for the second round of screening, and finally the target point andthe interacting site were based for the third round of screening. Result: The free binding energy of original ligand (4-[(5-bromo-4-{[2,4-dioxo-3-(2-oxo-2-phenylethyl)-1,3-thiazolidin-5-ylidene]methyl}-2-ethoxyphenoxy)-methyl]-benzoic acid, D77) was be used as positive control to screen out two natural micro-molecule compounds with good drug likeness. Thenatural micro-molecule compounds, HIV-1 intergraseandinteractive perssad showed a superioraffinity to D77 (a new-typeintergrase inhibitor). Their sources of traditional Chinese medicine were determined. Conclusion: This study successfully established a high-throughput virtual screening strategy for HIV-1 intergrase inhibitors, and provides an important reference and theoretical basis for the extraction of anti-AIDS compounds from Chinese herbal medicine and the design of anti-AIDS drugs.关键词：HIV-1 intergrase inhibitor;virtual screening;small molecule inhibitor
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To mine the clinical medication experience of marine Chinese medicine Sepiae Endoconcha accumulated during the development, in order to build the clinical medication experience mode of Sepiae Endoconcha, and provide the data support for the clinical application and further research of Sepiae Endoconcha. Method: Dictionary of Tradition Chinese Medicine Prescriptions and Encyclopedia of Traditional Chinese Medicine was chosen as basic information sources of formulas. Formulas containing Sepiae Endoconcha were screened, and their information was standardized. Frequency analysis, association rules mining technique and association network building technique were applied to analyze the selected formulas. Result: Totally 721 formulas were screened according to the criteria, involving 662 kinds of Chinese herbal medicines. The most frequently combined medicines with Sepiae Endoconcha were Os Draconis, Angelicae Sinensis Radix and Halloysitum Rubrum. Cuttlebone was usually combined with Trionycis Carapax and Aconiti Lateralis Radix Proeparata to treat malaria, with Angelicae Sinensis Radix to treat leucorrhea, and with Typhae Pollen to treat uterine bleeding. According to the association network, gynecological and ear,nose,throat(ENT) diseases, such as leucorrhea, pollen typhae and otitis media suppurativa, had very high clusters. According to the network relation map for the node of interstitial keratitis, Sepiae Endoconcha could be combined with camphol and white lilac to treat interstitial keratitis in the dosage form of ointment for external use. Conclusion: According to medication experience in Sepiae Endoconcha's main efficacies, clinical dosage and usage, and compatibility regularity accumulated by physicians of previous dynasties, this thesis is conducive to supplementing the knowledge of marine Chinese medicine Sepiae Endoconcha, and providing the reference and foundation for the modern clinical application and new drug development of Sepiae Endoconcha关键词：Sepiae Endoconcha;clinical medication experience mode;data mining
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To systematically review the efficacy and safety of Reduning injection for treating hand-foot-mouth disease (HFMD). Method: The Cochrane system evaluation method was used to retrieve such databases as the Cochrane Library, CBM, CNKI and Wanfang Data to collect the randomized controlled trials of Reduning injection in treating HFMD. The meta-analysis was performed using RevMan 5.2 software. Result: The 17 RCTs on Reduning versus Ribavirin involving 2 412 cases, as well as 7 RCTs on Reduning combined with Ribavirin involving 891 cases were included. The results of the Meta-analysis showed that the total treatment efficiency of Reduning group was better than that of Ribavirin[OR=4.71, 95%CI (3.46, 6.42), P<0.000 01], with a shorter fever clearance time[WMD=-1.20, 95%CI (-1.44,-0.95), P<0.000 01], skin rash regression time[WMD=-1.51, 95%CI (-1.95,-1.07), P<0.000 01], oral herpes or ulcer regression time[WMD=-1.36, 95%CI (-1.76,-0.97), P<0.000 01]and hospitalization time[WMD=-2.81, 95%CI (-3.79,-1.82), P<0.000 01], and significant differences. Compared to the Ribavirin group, the group of Reduning combined with Ribavirin was superior than the control group in the total treatment efficiency[OR=5.48, 95%CI (3.06, 9.80), P<0.000 01], fever clearance time[WMD=-1.70, 95%CI (-2.05,-1.35), P<0.000 01], skin rash regression time[WMD=-1.91, 95%CI(-2.99,-0.83), P<0.000 01], oral herpes or ulcer regression time[WMD=-1.81, 95%CI(-2.09,-1.53), P<0.000 01]and hospitalization time[WMD=-2.32, 95%CI (-3.59,-1.05), P=0.000 3], with significant differences. The 24 reports showed that the ADR/ADE of Reduning were lighter than that of Ribavirin, with a higher safety. Conclusion: Actual clinical evidence indicated that the efficacy and safety of the administration with Reduning alone and Reduning combined with Ribavirin was better than the administration with Ribavirin alone in the treatment of HFMD. However, because the reliability of systematic review is restricted by low quality of included study, more high-quality, large-scale and multiple-centered randomized controlled trials should be carried out for further verifying the conclusion.关键词：Reduning injection;hand-foot-mouth disease;Meta-analysis
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To analyze the prescription experience of professor BI Rong-xiu for steroid-induced avascular necrosis of femoral head (SANFH) based on traditional Chinese medicine (TCM) inheritance support system(TCMISS). Method: The prescriptions for SANFH were collected. The data was input into the TCMISS, and the composition principles were analyzed by using data mining methods, such as revised mutual information, complex system entropy cluster and unsupervised hierarchical cluster. Result: Based on the analysis of 61 eligible prescriptions, the frequency of each herb and association rules among the herbs were computed, and the most commonly used Chinese herbs, 4 core combinations and 6 new prescriptions were determined from the database. Conclusion: Professor BI Rong-xiu focused on the therapy of promoting blood circulation and removing blood stasis, nourishing liver and kidney in the treatment of SANFHT. In addition, according to different accompanied symptoms, professor BI Rong-xiu chose herbs with the efficacy of promoting Qi circulation and relieving pain, supplementing Qi and nourishing yin, and promoting bone, in order to achieve the treatment of both root causes and symptoms. The TCMISS is scientific and practicable to provide a comprehensive and objective analysis on the herbal prescriptions from famous TCM doctors, and so could be used to analyze famous TCM doctors' clinical experience.关键词：steroid-induced avascular necrosis of femoral head;Traditional Chinese Medicine Inheritance Support System (V2.5);herbal administration experience;BI Rong-xiu
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Bushen compound which is guided by traditional Chinese medicine (TCM) theory is considered to have potential therapeutic value for Alzheimer's disease, and it has gradually obtained attention from scholars both at home and abroad. In the study of last ten years, Bushen compound had better prevention and treatment effects for Alzheimer's disease. It could not only significantly improve cognitive dysfunction in dementia animal models, but also have positive effects for the pathogenic factors-included histopathology changes in the brain. Considerable efficacy has been obtained for some Bushen compound prescriptions in clinical observation. The nootropic and anti-senile dementia effects of Bushen compound may involve one or more biological mechanisms. Researchers have made extensive research on Bushen compound, but the characteristics of traditional Chinese medicine is not highlighted.关键词：Bushen compound;noortropic effect;Alzheimer's disease;brain neurotransmitter;Aβ inhibitor;immunotherapeutic agent
- 4
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Stachydrine, one of the main ingredients of Motherwort, could be artificially synthesized. Motherwort contains alkaloid 0.11%-2.09%, including 0.59%-1.72%stachydrine. Stachydrine is used as the indexes for quality control of currently commercial Motherwort liquid, Motherwort granule and Motherwort cream. In recent years, the research on their pharmacology is abundant. By retrieving all the literature about pharmacology of stachydrine worldwide in recent 10 years, it is concluded that stachydrine has the action of exciting uterus, anti-inflammatory, improving blood rheology, and anti-thrombus., providing values to further develop of stachydrine.关键词：stachydrine;pharmacological action;research progress;exciting uterus;anti-inflammatory;improving blood rheology;anti-thrombus.
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Compatibility of traditional Chinese medicine (TCM) is the basis, core and a key area in the research of Chinese medicinal formulae. Codonopsis Radix is a type of commonly used bulk medicinal materials in our country, and has extensive application values after compatibility; it is showed mainly synergistic or antagonistic effects, which finally influences the clinical effect. Therefore, studies on the compatibility of Codonopsis Radix have a very high value. In recent years, many experimental studies have been conducted on the compatibility of Codonopsis Radix. In this paper, relevant domestic and foreign studies on the compatibility of Codonopsis Radix in recent years were reviewed, processed and analyzed to lay a good foundation for further study and clinical application, and provide reference basis for application studies on Codonopsis Radix and relevant scientific workers.关键词：Codonopsis Radix;compatibility study;antagonistic effect;synergistic effect
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Traditional Chinese medicine Fibraureae Caulis boasts efficient ingredients, with low side effects and increasingly wide application value. Based on the analysis of relevant literatures at home and abroad, this article reviewed the study progress of the chemical composition and pharmacological effect of Fibraureae Caulis, and provided the reference for relevant in-depth studies. According to this study, Fibraureae Caulis contained a variety of alkaloids and lactones, as well as a few chemical constituents, such as terpenes, aldehydes,and essential oils. Among them, the main alkaloids were palmatine and jatrorrhizine, and lactones mainly included fibralactone. On the basis of the study on the pharmacological activity, Fibraureae Caulis' extracts and their chemical constituents showed various pharmacological effects, including anti-inflammation, anti-bacteria, anti-virusand improving immunity and memory. And palmatine was the research emphasis, and called ‘plant antibiotics’ by modern medicine. Along with the domestic and foreign discovery of new action targets and new efficacies, palmatine will have great demands in the international market. However, there is still a lack of in-depth study on palmatine's anti-inflammatory and anti-bacterial effects, and the chemical composition also requries further studies. This paper systematically reviewed the chemical constituents and pharmacological activities of Fibraureae Caulis, which will provide the reference for further studies on Fibraureae Caulis, facilitate sufficient development and utilization of natural medicine resources, broaden the new development prospect in the respect of botanical drug, and provide scientific basis in safe and efficient clinical application.
- 2
- |
- 2
- |
- 7
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0