最新刊期
卷 22 ， 期 17 ， 2016
- 摘要：Objective: To study on difference of toxicity of Kansui Radix before and after being processed with vinegar on normal and cancerous ascites model rats. Method: The normal and cancerous ascites model rats were orally administered with powder and extract of crude and stir-baked with vinegar of Kansui Radix for 7 d, respectively.The injury of liver, stomach, intestinal tissues of rats were observed through pathological section and the influence on biochemical indexes in serum was investigated. Result: Compared with the control group, these normal administration groups showed significant damage in liver, stomach and intestinal tissues, activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in serum were significantly increased, contents of glutathione (GSH) and superoxide dismutase (SOD) were significantly decreased, contents of malondialdehyde (MDA) and lactate dehydrogenase (LDH) were significantly increased;among these groups, the powder of crude Kansui Radix group showed the most pronounced effect, and the effect decreased significantly after being processed with vinegar.Compared with the model group, the injury of these model administration groups reduced significantly, activities of ALT, AST, ALP in serum were significantly decreased, contents of GSH and SOD were significantly increased, and contents of MDA and LDH were significantly decreased;among these groups, the powder of crude and stir-baked with vinegar of Kansui Radix were the most effective, and there was no significant difference between them. Conclusion: In normal rats, the toxicity of Kansui Radix decreases after being processed with vinegar;and in cancerous ascites model rats, the toxicity of crude and vinegar stir-baked Kansui Radix both decrease, and it can significantly alleviate the damage of the model.关键词：KANSUI RADIX;stir-baked with vinegar;cancerous ascites;toxicity;Yougu Wuyun;liver function
- 2
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To compare protective effect of raw and processed products of Gardeniae Fructus on acute hepatic injury induced by CCl4 in rats. Method: SD rats were evenly randomized into 9 groups by weight, such as normal group, bifendate group (35 mg · kg-1), model group, low and high dose of raw products of Gardeniae Fructus (2.0, 1.0 g · kg-1) groups, low and high dose of fried products of Gardeniae Fructus (2.0, 1.0 g · kg-1) groups, low and high dose of stir-baked products of Gardeniae Fructus (2.0, 1.0 g · kg-1) groups.Twelve hours after modeling, serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and total protein (TP) were examined.Super oxide dismutase (SOD) activity and malondialdehyde (MDA) content in liver tissue were determined.Histopathological changes of liver were observed. Result: Compared with the normal group, the model group significantly increased levels of AST, ALT, LDH, MDA and decreased level of TP and SOD activity.Liver cells arranged disordered, swelling and necrosis of liver cells were obvious.Compared with the model group, levels of AST, ALT and LDH in serum of raw and processed products groups decreased significantly, the raw and fried products groups were better.Raw and processed products groups increased activity of SOD and decreased the level of MDA significantly.There were no significant difference between the model group and administration groups in serum TP level of rats.Each administration groups reduced the number of degeneration, necrosis of liver cells and improve inflammatory cells infiltration. Conclusion: The raw, fried and stir-baked products of Gardeniae Fructus have an effective protection on acute hepatic injury induced by CCl4, the the raw and fried products are better in reducing enzyme level and antioxidant capacity.The fried products can decrease the bitter and cold of raw products.So the fried products of Gardeniae Fructus may be more appropriate in clinical application.关键词：raw products of Gardeniae Fructus;fried products of Gardeniae Fructus;stir-baked products of Gardeniae Fructus;hepatic injury;carbon tetrachloride;total protein
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To study oncontent variation of 5-hydroxymethylfurfural(5-HMF) in bran-frying process of Atractylodis Macrocephalae Rhizoma, and investigate its correlation with temperature and color changes of decoction pieces. Method: Atractylodis Macrocephalae Rhizoma was fried with stir-frying machine, and sampled at different time points.HPLC was applied to determine the content of 5-HMF with mobile phase of methanol-water (5:95) and detection wavelength at 284 nm.Two-dimensional infrared imager was adopted to measure the temperature of decoction pieces at different time points;color difference meter was empolyed to measure the color of decoction pieces at different time points. Result: 5-HMF in raw products of Atractylodis Macrocephalae Rhizoma was not detected.As bran-frying time extended, 5-HMF content increased slowly, when stir-frying about 15 min and temperature of decoction pieces at 150℃, 5-HMF content sudden rise sharply, and at this time, the color b* values of decoction pieces entered a significant decline phase. Conclusion: The content of 5-HMF changes regularly in the bran-frying process of Atractylodis Macrocephalae Rhizoma, and it has a remarkable correlation with temperature and color changes of decoction pieces.关键词：Atractylodis Macrocephalae Rhizoma;bran-frying;5-hydroxymethylfurfural;decoction pieces;temperature;color
- 3
- |
- 2
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To determine equilibrium solubility of isorhamnetin in water and 1-octanol, then calculate thermodynamic parameters, which might be important for its further studies on formulation development and in vivo absorption. Method: HPLC was established to determine equilibrium solubility of isorhamnetin in water and 1-octanol at different temperatures and its apparent partition coefficient in 1-octanol-water solution system. Result: Solubility of isorhamnetin in water and 1-octanol increased with increasing of temperature.Isorhamnetin was more soluble in 1-octanol than in water.Solubility data of isorhamnetin were fitted with Apelblat classic model, its regression equations in water and 1-octanol were lnxcal=879.18-53895.05/T-126.30lnT (r=0.9997), lnxcal=-1.32-2699.36/T+0.35lnT (r=0.9998).The dissolution processes in water and 1-octanol over the experimental temperature range were endothermic with increasing of entropy.The apparent oil-water partition coefficient of isorhamnetin at room temperature was 5163(lgP=3.71). Conclusion: Isorhamnetin is a fat soluble compound, its water-solubility is poor.By optimizing preparation process and other methods can improve its oral absorption efficiency and bioavailability.关键词：isorhamnetin;solubility;thermodynamics;apparent oil-water partition coefficient;1-octanol
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To optimize deproteinization technology of Dendrobii Officinalis Caulis.Method: With retention rate of total polysaccharides and deproteinized rate as the indexes, single factor tests were adopted to compare Sevage method, papain enzymatic, trypsin method and trypsin combined Sevage method.Effects of enzymolysis time, enzyme dosage, enzymolysis temperature and volume ratio of coarse polysaccharide solution to Sevage reagent on deproteinization technology was optimized by response surface method. Result: Trypsin combined Sevage method had higher retention rate of total polysaccharides and deproteinized rate;optimum deproteinization technology was as follows:enzymolysis time of 1.5 h, enzyme dosage of 0.21 g · mL-1, enzymolysis temperature at 63.25℃, coarse polysaccharide solution-Sevage reagent (4.27:1).Under these conditions, deproteinized rate and retention rate of total polysaccharides were (87.15±7.93)% and (81.32±8.54)%. Conclusion: Effect on total polysaccharides from Dendrobii Officinalis Caulis has significant difference with different deproteinized method, Trypsin combined Sevage method has optimum deproteinized effect.关键词：Dendrobii Officinalis Caulis;total polysaccharides;deproteinization;Sevage reagent;retention rate
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To determine equilibrium solubility of capsaicin in different menstruums and investigate drug loading property of thermoplastic polyurethanes (TPU). Method: Excess capsaicin was mixed in pure water, ethanol solution, glycerol solution and propylene glycol solution, respectively, and was shaken for 24 h.UV was employed for determination of capsaicin dissolved in these solvents and the equilibrium solubility was calculated.What's more, the equilibrium solubility of capsaicin in these tested menstruums under the conditions of different temperatures and pH values was also assayed.After that, drug loading behavior of TPU in various menstruums containing capsaicin with different liquid temperature as well as pH values were evaluated. Result: At room temperature, the largest equilibrium solubility of capsaicin was achieved in 50%propylene glycol solution.As temperature increased, the equilibrium solubility was risen.The acidity of solvent impacted the equilibrium solubility, especially pH value of capsaicin in the high concentration of short-chain alcohols.The drug loading capacity of TPU was effected by the liquid temperature and pH values of the tested menstruums. Conclusion: The 50% propylene glycol solution holds the largest equilibrium solubility of capsaicin, which positively correlates with temperature.In addition, pH values of these tested solvents should be noticed in evaluation of this alkaloid in these tested menstruums.Increasing drug concentration in menstruum will contribute to improve TPU loading with capsaicin.关键词：capsaicin;equilibrium solubility;thermoplastic polyurethanes;glycerol;propylene glycol;drug loading property
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To provide the fundamental basis for determining suitable planting areas of Lonicerae Japonicae Flos by analyzing and comparing the effect of ecological factors on active ingredients of Lonicerae Japonicae Flos. Method: Totally 11 samples of Lonicerae Japonicae Flos were collected from different areas with altitudes ranging from 4-2100 m, 10 bioactive contents of Lonicerae Japonicae Flos were obtained by HPLC. Meanwhile, climatic factors of different areas were collected, and the resulting data were statistically analyzed using SPSS 17.0 statistical software for the correlation analysis and the stepwise linear regression analysis. Result: There were significant differences among active ingredients of Lonicerae Japonicae Flos from different areas. Altitude is the dominant ecological factor influencing on the contents of chlorogenic acid and luteoloside. With the increase of altitude, the contents of chlorogenic acid and luteoloside showed a trend of increase. The content of caffeic acid was significantly positively related with the highest temperature in July, and negative related with the sunshine time. The content of isochlorogenic acid A and rainfall were significantly negative related, the content of quercetin and the minimum temperature in January showed a significant positive correlation. Conclusion: According to the results of this experimental study, we can speculate that with the increase of altitude in the planting areas of Lonicerae Japonicae Flos, the flowering temperature reduces, which is more conducive to the synthesis of chlorogenic acid and luteoloside.关键词：Lonicerae Japonicae Flos;bioactive component;ecological factor;correlation analysis
- 2
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To develop the method of Ultraviolet spectrum quad (UV)-chemical pattern recognition for the identification of Ligustri Lucidi Fructus and the fruit of Ligustrum japonicum. Method: Ligustri Lucidi Fructus and the fruit of L. japonicum were exact parallelly by four solvents including petroleum ether, methylene chloride, ethanol and water. Ultraviolet spectra of each fraction was scanned and the absorbance at different wavelengths was extracted at regular intervals for principle component analysis, Q-type clustering analysis and Fisher discrimination analysis. Result: Based on the results of principal component analysis and the Q-type clustering analysis, the discriminant function was established as follows:Y=-0.810+5.035Z1, p+3.645Z2, p-5.992Z3, p-5.609Z1, m+3.569Z2, m+6.519Z3, m-54.267Z1, e+6.901Z2, e+35.281Z3, e+13.334Z4, e+12.349 Z1, w-35.806.Z2, w. The accuracy of retrospective assessment was 100% in our experiment, and the UV-chemical pattern recognition method was established to identify Ligustri Lucidi Fructus and the fruit of L. japonicum. Conclusion: This method is scientific, accurate and simple, which can be used for the quick identification of Ligustri Lucidi Fructus and the fruit of L. japonicum.关键词：Ligustri Lucidi Fructus;the fruit of Ligustrum japonicum;UV;chemical pattern recognition
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To study the difference of chemical components in Ixeris sonchifolia (ISH) from different origins by UPLC-Q-TOF-MS. Method: The separation was performed on a Waters Acquity BEH C18 column (2.1 mm×100 mm, 1.7μm) with acetonitrile-0.1%formic solution as the mobile phase for gradient elution. Mass spectrometric data were obtained in electrospray ionization (ESI) positive and negative modes. Meanwhile, the chemical components were identified with quasi-molecular ions and secondary fragment ions. Principal component analysis (PCA) and partial least squares discriminate analysis (PLS-DA) were used for multivariate statistical analysis. Result: Eighteen chemical components were identified from ISH. Among them, 11, 13α-dihydroixerin Z, luteolin, 3, 4-dihydroxybenzoic acid, apigenin-7-O-glucuronide, rutin, chicory acid, luteolin-7-O-glucuronide and guanosine were the main differential compositions of ISH from different origins. Conclusion: The difference chemical constituents in ISH from different origins were identified by the established UPLC-Q-TOF-MS-MS method, which could provide the basis for the quality control of ISH.
- 2
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Atractylodis Macrocephalae Rhizoma. Method: The compounds were isolated and purified from ethyl acetate extract of Atractylodis Macrocephalae Rhizoma by using a combination of chromatographic techniques. Their structures were elucidated on the basis of thierphysico-chemical properties and spectroscopic data analyses. MTT method was used to assay the cytotoxicity agianst HeLa cells. Result: Sixteen compounds were isolated and purified from ethyl acetate extract of Atractylodis Macrocephalae Rhizoma and determined as 1-acetoxytetradeca-6E, 12E-dien-8, 10-diyn-3-ol (1), 3-acetoxytetradeca-6E, 12E-dien-8, 10-diyn-1-ol (2), tetradeca-6E, 12E-dien-8, 10-diyn-1, 3-diol diacetate (3), 14-acetoxy-12-senecioyloxytetradeca-2E, 8Z, 10E-trien-4, 6-diyn-1-ol (4), 14-acetoxy-12-senecioyloxytetradeca-2E, 8E, 10E-trien-4, 6-diyn-1-ol (5), atractylenolide Ⅰ (6), atractylenolide Ⅱ (7), atractylenolide Ⅲ (8), biatractylenolide (9), juniper camphor (10), taraxerylacetate (11), 3β-acetoxy-12-oleanen-11-one (12), 7-hydroxy coumarin (13), octacosanic acid (14), heptadecanoic acid (15), 7-α-hydroxyl-β-sitosterol (16). Conclusion: Among them, compounds 1, 2, 16 were isolated from the species firstly and compounds 12-15 were isolated from the genus of Atractylodes for the first time. Compound 6 showed good inhibitory activity against HeLa cells with IC50 values of 15.6μmol ·L-1.关键词：Atractylodes Macrocephala;1-acetoxytetradeca-6E,12E-dien-8,10-diyn-3-ol;Atractylodes;cytotoxicity;7-α-hydroxyl-β-sitosterol
- 2
- |
- 2
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To study the HPLC fingerprint of the total alkaloids from different parts of the Sophora alopecuroides, and provide the basis for scientific evaluation and effective control of the quality of total alkaloids in S. alopecuroides. Method: Totally 16 batches of S. alopecuroides from different producing areas and the total alkaloids were used to gain fingerprints by HPLC-DAD. The obtained data was analyzed by similarity evaluation, and the pattern recognition was carried out by cluster analysis and principal component analysis. Result: Fingerprints of S. alopecuroides fructus and herba S. alopecuroides extracts were established; common peaks were determined, and the chromatographic peaks were identified. Through cluster analysis and principal component analysis, the total alkaloids extract of the 16 batches Chinese medicinal materials were divided into 3 groups according to different producing areas. Conclusion: S. alopecuroides fructus and herba S. alopecuroides extracts had good fingerprint features and specificity, which could be used for the overall quality control of the total alkaloids from S. alopecuroides fructus and herba S. alopecuroides.关键词：Sophora alopecuroides;fructus;over ground;extract;total alkaloids;fingerprint;cluster analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for the simultaneous determination of prim-O-glucosylcimifugin, ferulic acid, naringin, menthone, osthole, and isoimperatorin in Jingfang granules. Method: The chromatographic separation was achieved on a ZORBAX SB-C18(4.6 mm×250 mm, 5μm) column with acetonitrile-water as mobile phases for gradient elution, at the flow rate of 1.0 mL · min-1, and the column temperature was 30℃. Result: The results showed that the six active components were well separated and showed good linear relationship within the following range, prim-O-glucosylcimifugin (5.5-27.4 mg · L-1), ferulic acid (6.5-32.3 mg · L-1), naringin (60.6-303.0 mg · L-1), menthone (0.48-2.4 mg · L-1), osthole (0.98-4.9 mg · L-1), and isoimperatorin (3.5-17.5 mg · L-1). The average recovery was between 97.5%-101.3%. Conclusion: This method is accurate, sensitive, credible and repeatable. It can be applied for the quality control of Jingfang granules.关键词：Jingfang granule;prim-O-glucosylcimifugin;ferulic acid;naringin;menthone;osthole;isoimperatorin
- 2
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To establish GC-MS fingerprint determination method of volatile oil from Dendranthema indicum var. aromaticum, and provide the reference for the quality control of D. indicum var. aromaticum medicinal materials. Method: The steam distillation method was used to extract the volatile oil from D. indicum var. aromaticum; the combined technology of GC-MS was used to determin its fingerprints, and Fingerprint Calculation Software of traditional Chinese medicine recommended by State Drug Administration was used for calculation, in order to establish the common fingerprints of volatile oil from D. indicum var. aromaticum. Result: There were 23 main characteristic components in the volatile oil from D. indicum var. aromaticum, and GC-MS fingerprint with these 23 common peaks was established preliminarily. Conclusion: The method is repeatable, accurate and stable, which can be used for quality control of the D. indicum var. aromaticum.关键词：Dendranthema indicum var. aromaticum;volatile oil;GC-MS;fingerprint
- 2
- |
- 5
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a rapid HPLC-DAD method for the simultaneous determination of four compounds, namely picroside Ⅰ, Ⅱ, paeoniflorin and albiflorin std in Qinggan Shuyu granule. Method: The analytes were extracted by ultrasonic. The separation of the compounds was performed on Diamonsil (2)-C18 (4.6 mm×250 mm, 5μm)column with acetonitrile-0.05%formic acid solution as mobile phase at the wavelength of 264 nm (picroside Ⅰ, Ⅱ) and 230 nm (paeoniflorin, albiflorinstd). Result: The four analytes demonstrated a good linearity (r>0.9991) within a relatively wide concentration range (0.0672-6.72μg for picroside Ⅰ, 0.0174-17.4μg for picroside Ⅱ, 0.01-10.00μg for paeoniflorin and 0.06-6.00μg for albiflorin std), and the recoveries ranged between 97.90%-100.95%, with RSD of 1.7%, 2.4%, 1.4%, 1.7%, respectively. Conclusion: The method was found to be accurate, simple and sensitive for the quantitative analysis on the four compounds in Qinggan Shuyu granule.关键词：Qinggan Shuyu granule;picroside Ⅰ;picroside Ⅱ;paeoniflorin;albiflorin std
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Tibet medicine Corydalis hendersonii. Method: The chemical constituents were isolated through combined techniques, silica gel, Sephadex LH-20, preparative TLC and semi-preparative HPLC, and their structures were determined on the basis of physicochemical properties, MS and NMR spectroscopic analysis and literature data. Result: Eleven alkaloids were isolated from the total alkaloids part of ethanol extract from C. hendersonii. Their structures were identified as 9-methyldecumbenine C(1), protopine(2), allocrytopine(3), stylopine(4), tetrahydroberberine(5), tetrhydropalmatine(6), tetrahydrocolumbamine(7), ochotensimine(8), fumariline(9), dihydrosanguinarine(10), and berberine(11). Conclusion: Compounds 3 and 5-11 were obtained from this species for the first time.关键词：Corydalis hendersonii;alkaloids;chemical constituent;structural identification
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To detect the relative content changes of endogenous metabolites in plasma of BALB/c mice by GC-MS technology, and to explore the mechanism of Jinxin oral liquid against respiratory syncytial virus (RSV) pneumonia in vivo. Method: BALB/c mice were challenged intranasally with RSV to establish the RSV pneumonia model, then intragastric administration of Jinxin oral liquid with dose of 27.6 g · kg-1 · d-1.After 3 days of inoculation, five mice in each group were hanged and inferior lobe of right lung were collected to take histopathological examination.After continuously given Jinxin oral liquid for 7 days, plasma were collected to detect the endogenous metabolites by GC-MS (m/z 50-500) and possible metabolic pathways concerned were analyzed. Result: Thirty-two endogenous metabolites in plasma were detected, arachidonic acid, glutamine, lactic acid, D-glucose, urea and 1, 5-anhydro-D-sorbitol were differential metabolites among them.Metabolic pathways of archidonic acid, glutamine and pyruvate were the most concerned. Conclusion: The antiviral effect of Jinxin oral liquid against RSV pneumonia may be related to regulation of metabolic disorder caused by RSV.关键词：GC-MS;Jinxin oral liquid;plasma;respiratory syncytial virus pneumonia
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To identify prototype components and metabolites of Polygonum capitatum extract in rat urine after oral administration by UHPLC-Q-TOF/MS, and explore its direct acting substances and in vivo metabolic process. Method: The separation was performed on a RRHD Eclipse Plus C18 column with mobile phase of 1%formic acid aqueous solution-0.1%formic acid acetonitrile solution for gradient elution, flow rate was 0.3 mL · min-1, column temperature was set at 45℃.Using electrospray ionization (ESI) in negative ion mode with scanning range from m/z 50 to 1000.By comparing chromatograms difference between blank urine and urine of administration, prototype components and metabolites of P.capitatum extract were identified. Result: Three kinds of prototype constituents and 15 kinds of metabolites were identified in the urine.These direct acting substances were protocatechuic acid, quercetin and gallic acid, or their products of liver metabolism. Conclusion: This method is reliable and effective for identifying compounds in urine after oral administration of P.capitatum extract, and it can provide a reference for further study on pharmacodynamic material basis of this herb.关键词：Polygonum capitatum;protocatechuic acid;quercetin;metabolites;metabolic pathway;gallic acid
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Anshen Dingzhiling (ADL) compound on signaling pathways of cyclin-dependent kinase 5(CDK5)/dopamine and adenosine 3', 5'-monophosphate-regulated phospho-protein, 32 kDa (DARPP-32)/protein phosphatase 1(PP1) in striatum and prefrontal cortex of spontaneously hypertensive rats (SHR) with attention deficit hyperactivity disorder (ADHD). Method: The 10 Wistar Kyoto rats were used as normal group. 50 SHR rats were randomly divided into model group, methylphenidate (MPH) group (2 mg · kg-1by gavage), and ADL low, middle, and high dose groups (6.7, 13.4, 26.7 g · kg-1 respectively), n=10 in each group. The rats in various groups received corresponding drugs by ig administration, bid. The brain tissues of the rats were taken after 4 weeks of treatment. Real-time PCR, Western blot and immunohistochemistry were used to detect the protein and mRNA expression levels of CDK5, p35, DARPP32, PP1 and cAMP response element binding protein (CREB) in striatum and prefrontal cortex of SHR rats. Result: As compared with normal group, the protein and mRNA expression levels of CDK5, p35 and CREB in striatum and prefrontal cortex were significantly reduced in rats of model group (P<0.05, P<0.01), the expression levels of DARPP32 and PP1 were significantly increased (P<0.05, P<0.01). After treatment, the protein and mRNA expression levels of CDK5, p35 and CREB in striatum and prefrontal cortex were significantly increased in rats of MPG group and ADL groups as compared with the model group (P<0.05, P<0.01), while the expression levels of DARPP32 and PP1 were significantly reduced (P<0.05, P<0.01). Conclusion: The acts of SHR rats may be associated with the signaling pathway of CDK5/DARPP32/PP1.ADL can exert the therapeutic action by regulating the signaling pathway of CDK5/DARPP32/PP1.关键词：Anshen Dingzhiling compound;attention deficit hyperactivity disorder;spontaneously hypertensive rats;signaling pathway of cyclin-dependent kinase 5/dopamine and adenosine 3',5'-monophosphate-regulated phospho-protein Mr 32 kDa/protein phosphatase 1
- 2
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the action mechanism of Astragali Radix-Curcumae Rhizoma combined with cisplatin (DDP) for hepatic cancer. Method: Human hepatocarcinoma cells HepG2 were transplanted into nude mice to establish models, and the modeled mice were randomly divided into 8 groups:model group, cisplatin (DDP) group (2 mg · kg-1, ip), Astragali Radix-Curcumae Rhizoma high dose, middle dose and low dose groups (H, M, L, 12, 6, 3 g · kg-1 · d-1, ig), Astragali Radix-Curcumae Rhizoma high dose+cisplatin group (H+DDP), middle dose+cisplatin group (M+DDP), and low dose+cisplatin group (L+DDP), n=8 in each group. The effects of Astragali Radix-Curcumae Rhizoma combined with cisplatin on apoptosis of human hepatocellular carcinoma cells HepG2 were observed by TdT-mediated dUTP nick end labeling(TUNEL). The apoptosis related protein B-cell lymphoma-2(Bcl-2) was detected by enzyme linked immunosorbent assay (ELISA). Expressions of microRNA(miR)-122a, miR-221 and miR-151 in transplanted tumor tissues were determined by Real-time PCR. Result: As compared with the model group, the apoptosis of tumor cells was obvious, the tumor cell nucleus was brown yellow under the microscope, tumor cell nucleus was highly condensed and some cells were detached from the nucleus in H, H+DDP, M+DDP, and L+DDP groups (P<0.05, P<0.01). The apoptosis was most significant in H+DDP group. ELISA results showed that the contents of Bcl-2 in H, M, M+DDP, H+DDP and L+DDP groups were significantly decreased as compared with the model group(P<0.01), and the decrease extent was most significant in H+DDP group. The Real-time PCR results showed that the expressions of miR-122a could be significantly up-regulated in H, M, L, H+DDP, M+DDP, and L+DDP groups (P<0.01) and expressions of miR-221, miR-151 were significantly inhibited in H+DDP group (P<0.01) as compared with DDP group. Conclusion: Astragali Radix-Curcumae Rhizoma can significantly induce apoptosis of human hepatocellular carcinoma cells HepG2 in positive correlation with dosage.The mechanism may be related to significantly down-regulating the expression of Bcl-2, up-regulating the expression of miR-122a and down-regulating the expression of miR-221 and miR-151.The combination of Astragali Radix-Curcumae Rhizoma and DDP showed synergistic effect.关键词：Astragali Radix;Curcumae Rhizoma;hepatocellular carcinoma;apoptosis;microRNA;B-cell lymphoma-2
- 2
- |
- 2
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: To investigate the protection effect of Angelicae Sinensis Radix and Hedysari Radix polysaccharide (AHP) in acute myocardial ischemic rats. Method: Eighty male Wistar rats were randomly divided into sham operation group, myocardial ischemia group, 3, 6, 12 g · kg-1 AHP groups, n=16 in each group. The acute myocardial ischemia models were established by ligating the left anterior descending coronary artery of the rats.3, 6, 12 g · kg-1 AHP was used by intragastric administration in the rats with 24 h of myocardial ischemia. Then the samples were collected from the ras at 36 hours, and 14 days after treatment respectively. Serum creatine kinase (CK), creatine kinase MB (CK-MB), lactate dehydrogenase (LDH), and LDHI levels were detected by biochemical analyzer; the expressions of heat shock protein70(Hsp70), Hsp32 and vascular endothelial growth factors (VEGF) mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: As compared with the sham operation group, the electrocard iogram ST segment dynamics were increased in myocardial ischemia group(P<0.05); the contents of CK, CK-MB, LDH and LDHI were significantly increased in myocardial ischemia group at 36 h, and the expression levels of Hsp70, Hsp32 and VEGF mRNA were also incresaed either (P<0.05, P<0.01). As compared with the myocardial ischemia group, the contents of CK, CK-MB, LDH and LDH1 were significantly increased in various dose treatment groups at 36 h; and the expression levels of Hsp70, Hsp32 and VEGF mRNA were incresaed (P<0.05, P<0.01). Conclusion: The Angelicae Sinensis Radix and Hedysari Radix ultrafiltration substabce can protect myocardium on acute myocardial ischemia.关键词：Angelicae Sinensis Radix and Hedysari Radix polysaccharide;acute myocardial ischemic;Creatine kinase;lactate dehydrogenase;heat shock protein;vascular endothelial growth factor
- 2
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Shierwei Chuanjia tablets on immunological hepatic fibrosis in rat models. Method: The male SD rats were randomly divided into normal group, model group, positive drug group (colchicine tablets, 0.1 mg · kg-1), and Shierwei Chuanjia tablets groups (2.8, 1.4, 0.7 g · kg-1). In all the other groups except normal group, pig serum was used to prepare the rat models of immunological hepatic fibrosis. At the same time, the rats received corresponding anti hepatic fibrosis interventions for a total of 6 weeks. The effects of drugs were observed by calculating the body mass and liver index, detecting liver function alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein(TP), albumin(ALB), globulin(GLO), and albumin globulin ratio (A/G). hyaluronate (HA) levels in serum were measured by using ELISA. HE staining was used to observe the pathological changes of liver tissues and Masson staining was used to observe the proliferation of collagen fibers. Result: As compared with the normal group, ALT and HA levels in serum of model rats were significantly increased, and liver indexes were also significantly increased (P<0.01); ALB and A/G were significantly decreased (P<0.01); the pathological changes of liver tissues were more obvious in model group. As compared with the model group, The other groups of Shierwei Chuanjia tablets had no significant effect on the changes of serum AST and GLO levels, but could significantly decrease the serum ALT and HA levels in liver fibrosis rats (P<0.01), increase the levels of serum TP, ALB and A/G, and can significantly reduce the pathological changes of liver tissues, with the most significant effect in 2.8 g · kg-1Shierwei Chuanjia tablets group. Conclusion: Shierwei Chuanjia tablets can significantly improve the hepatic fibrosis in model rats, showing certain anti-hepatic fibrosis effect.关键词：Shierwei Chuanjia tablets;pig serum;immunological hepatic fibrosis;livex index;liver function;hyaluronate
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To build with wild type and mutant integrins beta 1(integrin beta 1, ITGβ1)-3'end of translation region (UTR) dual luciferase reporter gene expression system (DLR) (pMIR-ITGβ1-3' UTR), through the dual luciferase report gene detects the kits ITGβ1, this study miR-29 ITGβ1 gene targeting regulatory role.Which based on regulating ITGβ1 transcription level after the screening of tanshinone. Method: Extraction of whole blood RNA, reverse transcriptase mRNA into cDNA, for the template, RT-PCR amplification ITGβ1-3'UTR fragments, after enzyme digestion to connect to the luciferase report carrier pMIR-Report, construct pMIR ITGβ1-3' UTR luciferase reporter gene vector and mutation carriers and identified.Will pMIR-Report Luciferase control, constructed pMIR ITGβ1-3'UTR and mutation carriers respectively with miR-mimics a total of 29 transfection to ovarian granulosa cells, with double Luciferase experimental analysis of the mechanism of action of miR-29 and ITGβ1. Result: By enzyme cutting and gene sequencing method confirmed by construction of plasmid sequence is correct;dual luciferase experiments have established that miR-29 can combine in ITGβ1-3'UTR.Successful build pMIR ITGβ1-3'UTR dual luciferase report gene, confirmed by the method of enzyme cutting and gene sequencing constructed plasmid sequence is correct.Cloning of DNA sequence fragment size and consistent with the reported in Genbank. Conclusion: The luciferase report gene build successful, miR-29 can combine after transcription ITGβ1-3'UTR.关键词：wild type and mutant;luciferase reporter gene;integrinsβ1-3'non-coding regions;dual luciferase reporter gene;transcription regulation;drug screening
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of total glucosides from Paeoniae Radix Alba (TGP) on endoplasmic reticulum stress and apoptosis in myocardial ischemia reperfusion rats. Method: Female Sprague-Dawley rats were used to establish myocardial ischemia reperfusion models and randomly divided into sham operation group, ischemia reperfusion (I/R) group, TGP low-dose group (ig 50 mg · kg-1 · d-1), TGP middle-dose group (ig 100 mg · kg-1 · d-1), and TGP high-dose group (ig 200 mg · kg-1 · d-1). Changes of cardiac function were observed in various groups; myocardial infarction size was detected by triphenyltetrazolium chloride (TTC) staining; levels of myocardial glucose regulating protein78(GRP78), CCAAT/EBP homologous protein (CHOP), Caspase-12 and Caspase-3 protien expression were measureed by Western blot; the apoptotic cardiomyocytes were assessed by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL). Result: As compared with the sham operation group, T-wave elevation and LVEDP were significantly increased; LVSP and +dp/dtmax were significantly reduced; and the protein expression levels of GRP78, CHOP, Caspase-12, and Caspase-3 were significantly increased in I/R group (P<0.01), with obvious myocardial infarction size and cells apoptosis. As compared with I/R group, T-wave elevation and LVEDP were reduced; LVSP and +dp/dtmax were increased; and the protein expression levels of GRP78, CHOP, Caspase-12 and Caspase-3 were reduced (P<0.05, P<0.01) in TGP groups in a concentration-dependent manner, with reduced myocardial infarction size and cells apoptosis. Conclusion: TGP can improve the cardiac function, myocardial infarction and apoptosis in myocardial ischemia reperfusion rats, and its mechanism may be associated with inhibiting the expression levels of GRP78, CHOP, Caspase-12 and Caspase-3.关键词：ischemia reperfusion;total glucosides from Paeoniae Radix Alba;apoptosis;glucose regulating protein78
- 2
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To study the effect of leonurine on myocardial ischemia and myocardial cell injury caused by acute myocardial infarction, and its functional mechanism. Method: The model of acute myocardial infarction in rats was established through ligation of the left anterior descending coronary artery. Male SD rats were randomly divided into five groups:sham-operated group, leonurine high-dose (30 mg · kg-1 · d-1) group, low-dose (15 mg · kg-1 · d-1) group, positive control group and model group. One week before the coronary artery ligation, all groups, except for the sham-operated group, were given the corresponding dose of drugs. The model was built on the eighth day, and postoperative intraperitoneal administration lasted for 2 days. The animals were sacrificed, and myocardial infarct size was measured by TTC staining. The content of lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde (MDA) in rat serum were determined with enzyme-linked immuno sorbent assay (ELISA) kit. Result: Compared with the sham-operated group, the model group showed significant increase in myocardial infarct size, and serum LDH, CK and MDA content, and significant decrease in total superoxide dismutase (T-SOD) (P<0.05, P<0.01); compared with the model group, the leonurine group showed significant decrease in myocardial infarct size, and serum LDH, CK and MDA content, and significant increase in total superoxide dismutase (T-SOD) (P<0.05, P<0.01). Conclusion: Leonurine has a good protective effect on myocardial injury caused by acute myocardial ischemia. Its mechanism may be related to the improvement of the antioxidant capacity of myocardial tissues.关键词：leonurine;acute myocardial infarction;myocardial ischemia;lactate dehydrogenase;creatime kimase
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Gei Herba on neuronal apoptosis and protein expressions in the peri-infarct cortex of rats with permenant middle cerebral artery occlusion, and investigate its possible mechanism. Method: Totally 72 SD rats were used to establish the models of permenant middle cerebral artery occlusion (pMCAO) by suture-occluded method, and they were randomly divided into sham-operation group, model group, Gei Herba high, middle and low dose groups (7.00, 3.50, 1.75 g · kg-1) and positive control group (Yinxingye tablet, 0.007 g · kg-1). The rats were administered with appropriate drugs intragastrically for 3 consecutive days.The nerve function scores were detected; extent of cerebral infarction was observed by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining; the pathological changes in peri-infarct cortex were observed by htoxylin eosin (HE) staining; neuronal apoptosis was detected by TUNEL staining; Bcl-2 and Bax protein expression levels were detected by immunohistochemical assay. Result: The nerve function scores and the extent of cerebral infarction were significantly increased; and the neurons were degenerated; the levels of Bax protein expression were increased and Bcl-2 protein expression were decreased of the peri-infarct cortex in pMCAO rats after cerebral ischemia for 3 d, with statistically significant differences as compared with the sham operation group (P<0.01). As compared with the model group, Gei Herba high, middle and low dose groups showed reduction in the nerve function scores and the cerebral infarction extent, improved the pathological changes in peri-infarct cortex, inhibited the neuronal apoptosis, promoted the protein expression of apoptin Bcl-2, and inhibited the protein expression of apoptin Bax (P<0.05). Conclusion: Gei Herba has certain protective effect on the cerebral ischemia injury in rats, and its mechanism may be related to increasing Bcl-2 level, inhibiting Bax activities in peri-infarct cortex, promoting anti-ischemia brain injury capacity and inhibiting apoptosis of nerve cells.关键词：Gei Herba;cerebral ischemia;cell apoptosis;B cell lymphoma/leukemia-2;Bcl-2 associated X protein
- 3
- |
- 2
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To observe the anti-fibrosis effects and mechanism of Schisandrae Chinensis Fructus caulis extract (SCFCE) on liver fibrosis in rats caused by CCl4 and alcohol. Method: Totally 50 Wistar rats were randomly divided into normal group, model group, SCFCE high, medium and low dose (5.0, 2.5, 1.25 g · kg-1) groups. Liver fibrosis models were established by intraperitoneal injection of CCl4, drinking alcohol and feeding high fat diets. After modeling, the rats in treatment groups were received different concentrations of SCFCE by intragastric administration, while the rats in the normal group and model group received the same volume of normal saline. After the last administration, the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), laminin (LN), hyaluronic acid (HA), and procollagen Ⅲ (PC Ⅲ) were detected, and their hepatic tissues were taken for HE staining. The protein expression levels of transforming growth factor-β1(TGF-β1), α-smooth muscle actin(α-SMA) were detected by Western blot assay. Result: As compared with the normal group, the levels of serum AST, ALT, HA, LN and PC Ⅲ were significantly elevated, and the protein expression levels of TGF-β1, and α-SMA in hepatic tissues were significantly increased in the model group. As compared with the model group, the levels of serum ALT, AST, HA, LN, PC Ⅲ were significantly decreased in SCFCE groups. Histopathological test results showed that SCFCE groups could significantly improve the heptic histological structure of rats, decrease the degree of liver fibrosis, and significantly reduce the protein expression levels of TGF-β1, α-SMA (P<0.01). Conclusion: SCFCE has obvious protective effects on CCl4 and alcohol-induced liver fibrosis in rats, and its mechanism may be related to decreasing the protein expression levels of TGF-β1, α-SMA, and inhibiting the proliferation of hepatic stellate cells.关键词：Schisandrae Chinensis Fructus;caulis extraction;transforming growth factor-β1;α-smooth muscle actin
- 2
- |
- 2
- |
- 10
发布时间：2024-01-04 - 摘要：Objective: To investigate the long-term clinical efficacy of lupus prescription on systemic lupus erythematosus (SLE), and further clarify the composition rules of the prescription, explore and enrich the theory of SLE in traditional Chinese medicine treatment system. Method: Retrospective, multi center test method was used, and 300 active SLE patients were randomly divided into treatment group and control group, 150 cases in each group. Patients in treatment group received hormone Prednison+Chinese herbal medicine lupus prescription, while patients in control group only received hormone therapy. The treatment was 6 months for both groups. Both groups were observed for changes of 24 h urine protein quantity, immune index, lupus activity scores before and after treatment, as well as the incidence of adverse effects, and the recurrence of the disease within half an year. Result: After treatment, the improvement of 24 h urinary protein, C4 and erythrocyte sedimentation rate (ESR) in treatment group was significantly better than that in control group (P<0.05). There was no significant difference in the improvement of the other indicators. The lupus activity scores in treatment group were significantly lower than those in control group (P<0.05). The incidence of side effects and recurrence rate of the disease in the treatment group were significantly lower than those in control group (P<0.05). Conclusion: Chinese herbal medicine lupus prescription has obvious long-term efficacy in treatment of SLE, especially in regulating immune function, promoting withdrawal of hormone, lessening the side effects of Western medicine and improving the therapeutic effect.关键词：lupus prescription;systemic lupus erythematosus;effects;mechanism;erythrocyte sedimentation rate;C4;24 h urinary protein
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the pathological and clinical discrepancy and clinic-pathology correlation of different traditional Chinese medicine (TCM) constitutions and different TCM syndrome patterns among patients with membranous nephropathy. Method: A cross-sectional study was used to collect the clinical data and analyze the correlation among TCM syndrome patterns, constitutions, pathological stages and laboratory results of 102 patients. TCM patterns were determined by a Chinese medicine physician according to the manifestation. Patients filled out the 'TCM Constitution Classification Table' to determine the constitutions. Result: There were five TCM syndrome patterns among 102 patients with membranous nephropathy. Syndromes of Qi deficiency of lung and kidney, Qi-Yin deficiency and Yin-Yang deficiency were excluded for comparison due to the small number of cases (only 4 patients). TCM constitutions of Qi stagnation and blood stasis, endowment quality were not found in the patients. Glassock stage was used to determine the renal pathology severity. The percentage of Qi deficiency constitution was lower than that of normal constitution, and the percentage of spleen and kidney Qi deficiency type dampness constitution (including dampness-heat constitution and dampness-phlegm constitution) was highest at membranous nephropathy stage Ⅱ (P<0.05). In clinical indexes:body mass index (BMI) of spleen and kidney deficiency type dampness constitution was higher than that of other constitutions (P<0.01). Serum creatinine of spleen and kidney Qi deficiency type dampness constitution was higher than that of normal constitution, and it was lowest in spleen and kidney Qi deficiency type Qi deficiency constitution (P<0.05). Serum creatinine of spleen and kidney Yang deficiency type Yang deficiency constitution was significantly lower than that of spleen and kidney Qi deficiency type dampness constitution (P<0.05). Triglyceride of spleen and kidney Qi deficiency type dampness constitution was significantly higher than that of spleen and kidney Yang deficiency type Yang deficiency constitution (P<0.01). High density lipoproteins (HDL-C) of spleen and kidney Qi deficiency type dampness constitution was significantly lower than that of spleen and kidney Yang deficiency type Yang deficiency constitution and spleen and kidney Qi deficiency type normal constitution (P<0.05). 24 h urine protein was highest in spleen and kidney Qi deficiency type dampness constitution, followed by spleen and kidney Qi deficiency type normal constitution, spleen and kidney Qi deficiency type Qi deficiency constitution, and spleen and kidney Yang deficiency type Yang deficiency constitution (P<0.05). In immune indexes:Immunoglobulin G (IgG) of Yang deficiency constitution was higher than that of dampness constitution (P<0.05), and erythrocyte sedimentation rate (ESR) of Yang deficiency constitution was significantly higher than that of normal constitution (P<0.01). Conclusion: Patients with disparities of TCM constitutions and syndrome patterns may have a severe clinic-pathology results, while patients with similar TCM constitutions and syndrome patterns may have an opposite result. These suggested that TCM constitutions and syndrome patterns were correlated with clinical indicators. Different constitutions may present with different clinic-pathology results even in the same TCM syndrome pattern. Therefore, prevention and treatment of different constitutions should be discriminatory even in the same TCM syndrome pattern. The combination of TCM constitution and syndrome pattern may take a role in pathology prediction, diagnosis and treatment for membranous nephropathy.关键词：membranous nephropathy;traditional Chinese medicine (TCM)constitution;TCM syndrome pattern;clinic-pathology
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: Toreveal the traditional Chinese medicine (TCM) syndromerules of diabetic peripheral vascular disease, and provide reliable and objective basis for clinical diagnosis and treatment. Method: Based on the TCM symptoms, 898 patients with diabetic peripheral angiopathy were divided into 3 groups, Qi and Yin deficiency with blood stasis group, Qi and Yin deficiency with dampness-heat group and Yin and Yang deficiency group. Different tests were carried out to examine the differences among groups on fasting plasma glucose (FPG), 2 hour postprandial blood glucose (2 h PG), glycosylated hemoglobin (HbAlc), blood fat, fibrinogen, packed red cell volume and vascular ultrasound, et al. Result: There was no difference in the levels of FPG, 2 h PG and HbAlc among groups. The levels of waistline, hip circumference, waist/hip ratio, body weight, body mass index (BMI), triglyceride, cholesterol, low density lipoprotein, fibrinogen and packed red cell volume were significantly higher in Yin and Yang deficiency group (P<0.05, P<0.01), followed by Qi and Yin deficiency with dampness-heat group, and the values were lowest in Qi and Yin deficiency with blood stasis group. Vascular ultrasound showed that the blood flow volume of dorsal artery of foot in Yin and Yang deficiency group was significantly lower than that of Qi and Yin deficiency with dampness-heat group (P<0.05). In addition, calibers of femoral artery and popliteal artery were gradually narrowed and blood flow volume was gradually reduced in turn from Qi and Yin deficiency with blood stasis group, Qi and Yin deficiency with dampness-heat group to Yin and Yang deficiency group. Conclusion: The Yin and Yang deficiency was the most severe stage of diabetic peripheral vascular disease, followed by Qi and Yin deficiency with dampness-heatand then Qi and Yin deficiency with blood stasis.The occurrence regularity of diabetic peripheral vascular disease was revealed so as to provide reliable theoretical basis for clinical classification, and it was good for determining the treatment based on differentiation of symptoms and signs.关键词：diabetes;diabetic peripheral vascular disease;traditional Chinese medicine syndrome;rule
- 2
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To determine the clinical application value of Huaxian decoction by observing its clinical efficacy in treatment of hepatic fibrosis. Method: The 60 patients with hepatic fibrosis in gastroenterology and hepatology clinic and inpatient department of our hospital from January 2012 to December 2015 were included in the present study, and randomly divided into treatment group (30 cases) and control group (30 cases). Patients in treatment group received Huaxian decoction, and patients in control group received Silybin. Both groups were observed for clinical efficacy, and the changes in liver function, instantaneous elastic indexes in liver, hepatic fibrosis indexes, liver B-ultrasound, computed tomography (CT) or magnetic resonance imaging (MRI) and other objective indexes. Result: As compared with the conditions before treatment, the laboratory parameters such as transaminases, instantaneous elastic index in the liver, and hepatic fibrosis indexes were significantly reduced in both groups (P<0.05). The efficacy in treatment group was superior to that of control group after treatment (P<0.05). Conclusion: Huaxian decoction has certain effect in treatment of hepatic fibrosis, and further more multi-center and multi-link studies are required.关键词：Huaxian decoction;hepatic fibrosis;clinical observation;transaminases;instantaneous elastic index;hepatic fibrosis indexes
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effects of Jinshui Liujun Jian and San'ao Fang in assisting the western medicine for acute exacerbation of chronic bronchitis. Method: The 160 patients with acute exacerbation of chronic bronchitis were chosen in the period from May 2012 to May 2014 in Qinghai Hospital of Traditional Chinese Medicine(TCM), and they were randomly divided into control group (80 patients) with routine western medicine intervention and treatment group (80 patients) with Jinshui Liujun Jian combined with San'ao Fang on the basis of control group treatment. The clinical efficacy, the duration time of clinical signs and symptoms, the TCM syndrome scores, pulmonary function levels, inflammatory cytokine levels and the clinical recurrence rate of two groups were compared. Result: The clinical efficacy in treatment group was significantly better than that in control group (P<0.05). The TCM syndrome scores were significantly reduced after treatment in both groups (P<0.05), and the scores in treatment group were significantly lower than those in control group after treatment (P<0.05). The duration time of clinical signs and symptoms of treatment group was significantly less than that in control group (P<0.05). The levels of pulmonary function were significantly increased after treatment in both groups (P<0.05), and the levels in treatment group were significantly higher than those in control group after treatment (P<0.05). The levels of inflammatory cytokine were significantly decreased after treatment in both groups (P<0.05), and the levels in treatment group were significantly lower than those in control group after treatment (P<0.05). The clinical recurrence rate in treatment group was significantly lower than that in control group (P<0.05). Conclusion: Jinshui Liujun Jian and San'ao Fang in assisting western medicine for acute exacerbation of chronic bronchitis can efficiently alleviate clinical symptoms and signs, shorten the clinical course, improve lung functions, and help to reduce the risk of long-term recurrence.关键词：Jinshui Liujun Jian;San'ao Fang;chronic bronchitis;acute exacerbation;traditional Chinese medicine syndrome score;pulmonary function;inflammatory cytokine;clinical recurrence rate
- 2
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Zhuling Tang combined with Fuyuan Huoxue Tang in the treatment of severe closed renal injury and investigate its effects and clinical significance on serum interleukin (IL)-6, IL-8, IL-10 and tumor necrosis factor (TNF)-α levels in the course of treatment. Method: The 80 patients with severe closed renal injury were randomly divided into treatment group (40 cases) and control group (40 cases). Patients in control group received conventional western medicine treatment, and patients in treatment group orally took Zhuling Tang combined with Fuyuan Huoxue Tang based on the treatment in control group. The peripheral blood leukocytes, length of hospital stay, disappearance time of hematuria under microscope, beginning time of perirenal hematoma absorption and renal subcapsular hematoma absorption were observed. CT was taken in 2, 4, 12 weeks to observe the changes of perirenal hematoma, and the IL-6, IL-8, IL-10 and TNF-α expression levels were detected by enzyme-linked immunosorbent assay (ELISA) in 0.5, 1, 2, 3, 5, 7 d. Result: After treatment, the length of hospital stay, beginning time of perirenal hematoma absorption and renal subcapsular hematoma absorption, and disappearance time of hematuria under microscope in treatment group were significantly shorter than those in control group (P< 0.05), the declined number of white blood cells in peripheral blood was significantly higher than that in control group (P< 0.01), and the increased number of hemoglobin in peripheral blood was significantly superior to that in control group (P<0.01). At 2, 4 and 12 weeks after treatment, the absorption of perirenal hematoma in treatment group was significantly better than that of control group (P<0.01). The levels of IL-6, IL-8 and TNF-α in treatment group were significantly lower than those in control group in earlier periods (P<0.01), and the level of IL-10 in the treatment group was significantly higher than that in control group in earlier periods (P<0.01). Conclusion: Zhuling Tang combined with Fuyuan Huoxue Tang has obvious clinical efficacy in the treatment of closed renal injury, which can reduce the production of proinflammatory cytokines IL-6, IL-8 and TNF-α, promote the production of anti-inflammatory factor IL-10, and inhibit the inflammatory reaction of the patients. Therefore, its mechanism may be associated with regulating the cytokines and inhibiting inflammatory reaction.关键词：Zhuling Tang;Fuyuan Huoxue Tang;renal injury;interleukin;tumor necrosis factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Fuzheng Gubiao decoction combined with plastering therapy in treating recurrent respiratory tract infection (RRTI) and its influence on immunologic function and trace elements. Method: One hundred and fifty patients were divided into control group (75 cases) and observation group (75 cases) by random number table. Both groups were given antibiotic, antivirus, defervescence, and antitussive treatment and fluid infusion during acute infection period. Both group's patients took Pidotimod oral solution. Patients in control group received Yupingfeng oral liquid, 5-10 mL/time, 3 times/day. Patients in observation group took Fuzheng Gubiao decoction combined with plastering therapy. Courses of treatment lasted for 16 weeks. And a 32-week following-up was conducted. Onset frequency, days of morbidity and disease type were recorded within 48 weeks. Lung-spleen deficiency syndromes were scored. Levels of serum immunoglobulins IgA, IgG, IgM, T-lymphocyte, complement component C3 and C4, Ca, Fe, Zn, Mg and Cu were detected. Result: The total effective rate in observation group was 94.29%, which was superior to 82.09%in control group (P<0.05). During the 48-week following-up visit, mean onset frequency in observation group were less than that in control group, and mean disease course was shorter than that in control group, disease severity was also lower than that in control group (P<0.01). After treatment, levels of CD3+, CD4+ and CD4+/CD8+ in observation group were higher than those in control group, but level of CD8+was lower than that in control group (P<0.01). Levels of IgG, IgA and IgM were higher than those in control group (P<0.01). There were no statistical significant differences in levels of C3, C4 and Cu between the two groups. Levels of Ca, Fe, Zn and Mg in observation group were higher than those in control group (P<0.01). Conclusion: Fuzheng Gubiao decoction combined with plastering therapy can improve immunity, regulate levels of microelements, reduce onset frequency and onset degree of RRTI, with obvious long-term and short-term effects.关键词：recurrent respiratory tract infection;Fuzheng Gubiao decoction;plastering therapy;immune globulin;T-lymphocyte
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To discuss the short-term effect of modified Xiangbei Yangrong Tang based on differentiation and its influence on T-lymphocyte and natural killer cells in treating metastatic breast cancer treated. Method: Ninety patients were divided into control group (44 cases) and observation group (46 cases) by random number table. Patients in control group received endocrine therapy, as well as chemotherapy when necessary. In addition to the therapy of control group, patients in observation group were additionally administered with modified Xiangbei Yangrong Tang based on differentiation. The treatment was end when the disease progressed. Qi-blood deficiency, and accompanied symptoms and Kupperman were scored. Time to progression (TTP) was recorded, quality of life-liver cancer of breast cancer patients (FACT-B) and Keypunch Performance System (KPS) were adopted. Levels of T-lymphocyte and natural killer cells were detected, and adverse reactions were recorded. Result: The effective rate in observation group was 54.35%, which was superior to 31.82%in control group (P<0.05). TTP in control group was (5.12±1.04) mouths, which was lower than (7.91±1.41) in observation group (P<0.05). After treatment, scores of symptoms and Kupperman in observation group were lower than those in control group, but score of KPS was higher than that in control group (P<0.05). After the treatment, scores of physiological status, social and family status, emotional situation, functional status and additional attention in observation group were all lower than those in control group (P<0.01). In observation group, levels of CD3+, CD4+, CD4+/CD8+ and NK increased and were higher than those in control group (P<0.01), and level of CD8+decreased and was lower than that in control group (P<0.01). Adverse reactions caused by chemotherapy in control group was more serious than in observation group (P<0.05). Conclusion: Modified Xiangbei Yangrong Tang based on differentiation can prolong TTP, promote short-term effects on solid tumor, attenuate symptoms, improve patients' qualities of live and immune function and relieve adverse reactions caused by endocrine and chemotherapy therapy.关键词：metastatic breast cancer;chemotherapy;Xiangbei Yangrong Tang;time to progression;quality of life;T-lymphocyte;natural killer cells
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of Jinzhen oral liquid for respiratory tract infection by using network pharmacology method. Method: The structures of small molecule compounds of Jinzhen oral liquid were downloaded from database; molecular descriptors were calculated to study the chemical space distribution of these compounds. After that, the interactions between those compounds and target proteins related to respiratory infections were studied by molecule docking method. Then the "drug-target network" was constructed to predict the main active ingredients and action targets of Jinzhen oral liquid in the treatment of respiratory tract infection. Result: The 651 compounds of Jinzhen oral liquid showed good drug-like properties. Network analysis results showed that, 120 compounds of Jinzhen oral liquid were strongly interacted with 23 targets related to respiratory tract infection. Furthermore, 11 active compounds and 18 potential targets of Jinzhen oral liquid were predicted for treating respiratory tract infection. Conclusion: The effect of Jinzhen oral liquid for respiratory tract infection may be associated with the adsorption, replication, and uncoating of virus (influenza virus, nasal virus) infections, as well as interfering the inflammation related signaling pathways and regulating the secretion of inflammatory mediators.关键词：Jinzhen oral liquid;respiratory tract infection;network pharmacology;molecule docking;network analysis
- 5
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To analyze the medication rules of traditional Chinese medicine (TCM) prescriptions for treating Sjögren syndrome collected in CNKI, Wanfang and VIP based on TCM Inheritance Support System. Method: CNKI, Wanfang and VIP were searched to screen out clinical literatures about TCM prescriptions for treating Sjögren syndrome. TCM Inheritance Support System was employed to mine the data of TCM prescriptions for treating Sjögren syndrome. Result: Totally 258 literatures were selected, involving 355 prescriptions for Sjögren syndrome, and 323 traditional Chinese medicinal herbs, including 88 TCM herbs with the use frequency ≥10.Furthermore, 60 core combinations and 30 new prescriptions were evolved. Conclusion: Through the analysis on the literatures related to Chinese formula treating Sjögren syndrome in CNKI, Wanfang and VIP through the TCM inheritance support system, the method for nourishing Yin and promoting the secretion of body fluid is the basic method for treating Sjögren syndrome, meanwhile, the new formulations contain lots of drugs for dredging collaterals. All the conclusions provide new reference for clinical and scientific researches.关键词：Sjögren syndrome;prescription;medication characteristic;traditional Chinese medicine inheritance support system
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate the therapeutic effect and safety of umbilical therapy of traditional Chinese medicine(TCM) combined with routine western therapy in treating ascites due to liver cirrhosis. Method: Databases, such as CNKI, Wanfang, CBM, VIP, PubMed, Cochrane Library, were searched (as at March 2016) to screen out relevant journals and the literature of randomized controlled trials(RCT). The quality of RCT was assessed by using Jadad scores, and Meta-analysis was performed with Review Manager 5.3 software. Result: Twenty-three articles were enrolled, involving 1891 patients. The results of Meta-analysis showed that intervention measures could improve the clinical effect[OR=3.32, 95%CI (2.58, 4.27), P<0.00001], increase TCM symptom complex score[WMD=-1.85, 95%CI (-2.49, 1.20), P<0.00001], reduce abdominal circumference[WMD=-10.04, 95%CI (-12.84, -7.25), P<0.00001], et al. Conclusion: The combination of TCM and routine western therapy in treating ascites due to liver cirrhosis has a better efficacy and safe than the administration of routine western treatment along.关键词：umbilical therapy of traditional Chinese medicine;ascites due to liver cirrhosis;systematic review;Meta-analysis
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To analyze the composing principles of the prescriptions for tic disorders (TD) collected in CNKI and CSPD through Traditional Chinese Medicine(TCM) Inheritance Support System (TCMISS), in order to provide a reference for clinical treatment of TD. Method: The prescriptions used for TD in CNKI and CSPD from 1998 to 2015 were collected to build a database through TCMISS to make an analysis by association rules and complex system entropy clustering. Then the composing principles of the prescriptions for TD were analyzed. Result: Based on the analysis of 232 cases, the most frequently used drugs included Uncariae Ramulus Cum Uncis, Paeoniae Radix Alba, Bombyx Batryticatus, Glycyrrhizae Radix et Rhizoma and Gastrodiae Rhizoma. And most of these drugs entered the channel of liver. By analyzing the association rules, 16 core prescriptions were found, and seven new prescriptions were obtained through the cluster analysis. Conclusion: In the treatment of TD with TCM, invigorating the spleen, calming the liver, reducing phlegm and stopping endogenous wind are the major rules, and recuperating lung is the key. The TCMISS is of great help for the research of TCM prescriptions for TD.关键词：tic disorders;composing principles of prescriptions;data mining;Traditional Chinese Medicine Inheritance Support System
- 2
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Fitz-Hugh-Curtis (FHC) refers to pelvic infection combined with liver inflammation, and can be found in such diseases as pelvic inflammation, annex inflammation and infertility. Due to the low incidence and untypical symptoms, it is often ignored by doctors and patients in clinic. However, with the improvement in diagnostic means and attention from doctors, the majority of patients could receive timely diagnosis and treatment. To explore the FHC pathogenesis regularity, the key points of diagnosis and reliable therapies, we referred to domestic and foreign research literatures, started with the confirmation of diagnosis, made traditional Chinese medicine treatment(TCM) based on syndrome differentiation by means of combining TCM and western medicine and administering antibiotics according to patients' clinical manifestations. The purpose was to shorten the FHC treatment course and improve the cure rate. Confirmed FHC is often accompanied with some complications, such as hydrosalpinx, fallopian tube obstruction, and ovarian surrounding adhesion; besides, patients had a poor body disease-resistant ability. In this case, TCM has a certain advantage. According to patients' pulse conditions, FHC etiology and pathogenesis, key points for diagnosis, syndrome differentiation regularity, and medication skills, clinical doctors believed that FHC is correlated with liver Qi stagnation, dampness and heat in liver channel, phlegm-damp stagnation and congelation, and Qi-blood disharmony, respectively adopted therapies for relieving the depressed liver, regulating vital energy and dredging stagnation, relieving the liver and heat, removing damp and turbid, relieving phlegm and dampness, regulating vital energy and removing stagnation, supplementing Qi and nourishing blood, and strengthening body resistance and eliminating evil, and prescribed sensitive antibiotics to specific conditions. The clinical observation showed a good effect to pelvic infection and liver inflammation. Compared with the treatment with single TCM or western medicine, this therapy shows a better curative effect in removing pain from patients and restoring functions of infected organs, and obvious advantages. This exploration provides important reference for the combination of TCM and western medicine in the treatment of FHC.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Scutellaria baicalensis is one of the traditional Chinese medicine and its active ingredient flavonoids mainly, research shows that their active ingredients has good anti-inflammatory effects. At present, with the deepening of the inflammatory response, the researchers found that activation of the inflammatory response occurs with multiple cellular pathways related, so by blocking the pathway of inflammation, reduce the expression of key proteins in pathways activated, mitigation and treatment of inflammatory reaction is of great significance. Study show that baicalin, baicalein, Wogonoside and wogonin in vitro and in vivo inhibition of inflammation areclosely linked with regulation of a number of major inflammation pathway. Through literature, role of effective components of radix scutellariae in inflammatory pathway as a starting point, focuses on the effective components of radix scutellariae's role in the inflammatory pathway target proteins, provide references for further study of the anti-inflammatory effects of scutellaria baicalensis.关键词：Scutellaria Radix;inflammatory pathways;active ingredients;action target
- 3
- |
- 2
- |
- 12
发布时间：2024-01-04 - 摘要：Pine needles are the leaves of Pinus massoniana, P.elliottii, P. taiwanensis, Larix gmelinii, P. Thunbergii, P. Koraiensis and other plants, they distribute throughout the country and have a long pharmaceutical history.Polyprenol is one of the principal constituents in pine needles, which has similar structure and biological activities as dolichol in human body.Previous studies show that polyrenol possesses multiple pharmacological properties, such as anti-alzheimer, anti-oxidation, anti-liver injury, etc.Nowadays, some progress on its extraction, separation, purification and pharmacological activities has been demonstrated, however, research on its pharmaceutical preparations for clinical application is not still reported.Further research is helpful for the development of new medicines and making the most use of pine needles' resources.In order to provide valuable references of further research and development for polyprenol in pine needles, we review current research on extraction, separation, purification, determination, pharmacological effects, preparation of polyprenol.Based on the current studies, for the purpose of rational development and applied value, further studies shall be made for the technology of separation and purification, pharmacological mechanism, preparation of polyprenol.关键词：pine needles;polyprenol;extraction;separation and purification;pharmacological effects;preparation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0