最新刊期
卷 23 ， 期 19 ， 2017
- 摘要：Objective: To investigate the effect and mechanism of Xuesaitong(XST) on the apoptosis of human umbilical vein endothelial cells (HUVEC) induced by hydrogen peroxide. Method: HUVECs were cultured in vitro and H2O2 was used as oxidants to establish oxidative stress model in HUVEC. The changes of apoptosis induced by H2O2 in HUVEC cells were observed by XST intervention, and the protective effect of XST on HUVEC was discussed from the perspective of epigenetic miRNA. The cultured HUVECs were divided into blank control group, H2O2 model group, and H2O2 model+XST (30 mg·L-1, 24 h) group. The cell viability was detected by cell counting kit8 (CCK8) method; apoptosis was detected by Annexin V/Propidium iodide (PI); and the expression of miRNA-146 and miRNA-199 was detected by Real-time PCR. Result: After 100 μmol·L-1 H2O2 treatment, the late apoptosis and early apoptosis of HUVECs were significantly increased (P<0.05). Pretreatment of HUVEC cells with a dose of 1 mg·L-1 to 100 mg·L-1 showed that a significant improvement in cell proliferation was present in 10 mg·L-1 preconditioning. Apoptotic detection of Annexin V/PI double staining showed that the PI positive rate R3 (late apoptotic) and Annexin V positive rate R5 (early apoptosis) were significantly increased after HUVEC treatment with 100 μmol·L-1 H2O2 significantly (P<0.05). While the cell PI positive rate R3 (late apoptosis) and Annexin V positive rate R5 (early apoptosis) were significantly reduced after 30 mg·L-1 XST treatment in H2O2 models (P<0.05). The expression levels of hsa-miR-146b-5p and hsa-miR-199a-5p were significantly increased by H2O2 treatment at 100 μmol·L-1. As compared with H2O2 group, the expression of hsa-miR-146b-5p was significantly decreased after treatment with 30 mg·L-1 XST (P<0.05). Conclusion: XST can significantly resist H2O2-induced oxidative damage in HUVEC cells, which may play a protective role by inhibiting the increase of hsa-miR-146b-5p.关键词：human umbilical vein endothelial cells;Xuesaitong;oxidative damage;apoptosis;miRNA
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of xuesaitong (XST) for coronary heart disease unstable angina with blood stasis, and investigate its effects on expression levels of hsa-miR-199a-5p, hsa-miR-146b-5p, KIR3DS1, HLA-DPB1, TP53SESN2, NCR1, and PRF1 in peripheral blood and to elucidate the molecular mechanism of XST for the coronary heart disease with blood stasis. Method: The 80 patients with coronary heart disease unstable angina grade Ⅰ-Ⅲ with blood stasis were enrolled and randomly divided into treatment group and control group (40 cases in each group). The patients in control group received routine western medicine and placebo, while the patients in treatment group also received XST capsules on the basis of routine western medicine therapy. The treatment duration was 4 weeks for both groups. The levels of total ischemic burden, seattle quality score for angina pectoris, haemo-dynamics and blood lipid were observed before and after treatment to evaluate the clinical of XST capsules for coronary heart disease unstable angina with blood stasis. Besides, hsa-miR-199a-5p, hsa-miR-146b-5p, KIR3DS1, HLA-DPB1, TP53, SESN2, NCR1, and PRF1 were validated by Real-time PCR. Result: As compared with control group, total ischemic burden, whole blood viscosity, plasma viscosity, red blood cell rigidity index, cholesterol (TC), triglyceride(TG), and low density lipoprotein cholesterol (LDL-C) were decreased in treatment group (P<0.05) after treatment; KIR3DS1, TP53, SESN2, PRF1 genes were up-regulated significantly while hsa-miR-199a-5p, hsa-miR-146b-5p levels were down-regulated in treatment group (P<0.05). Conclusion: XST could ease the symptoms of patients with coronary heart disease unstable angina with blood stasis and the underlying mechanism might be associated with hsa-miR-199a-5p and hsa-miR-146b-5p.关键词：coronary heart disease;unstable angina;blood stasis;microRNA
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发布时间：2024-01-04 - 摘要：Objective: To conduct high throughput detection of microRNA expression in patients with non-ST segment elevation myocardial infarction (NSTEMI) before and after drug intervention, compare the expression changes of microRNA before and after treatment with Chinese and western medicines, and analyze the intervention targets and mechanism of Chinese medicines for promoting blood flow and eliminating blood stasis. Method: Patients who met the diagnostic criteria of Western medicine diseases and the diagnostic criteria of traditional Chinese medicine (TCM) syndromes were divided into NSTEMI Chinese medicine treatment group and NSTEMI western medicine control group, 35 cases in each group. Their peripheral blood was extracted, peripheral blood mononuclear cells (PBMC) were isolated and total RNA was extracted. The active microRNA and its target genes in each group were identified according to differentially expressed microRNAs, gene pathways and network analysis results. Result: In the microRNA expression profile, there were differences between Chinese medicine treatment group before and after treatment as well as between TCM treatment group and western medicine control group after treatment, hsa-miR-92a, hsa-miR-363, hsa-miR-499, hsa-miR-30b, hsa-miR-454-3p, and hsa-miR-933 expression levels were down-regulated, while hsa-miR-144, hsa-miR-451, hsa-miR-494, and hsa-miR-320 expression levels were up-regulated in TCM treatment group after treatment. These 10 microRNAs were considered as the intervention targets of salvianolate for NSTEMI. Real-time PCR, results verified that as compared with the western medicine control group, hsa-miR-92a, hsa-miR-30b, hsa-miR-499 and hsa-miR-454 expression levels were down-regulated while the expression of hsa-miR-494 was up-regulated after treatment in Chinese medicine treatment group (P<0.05). Conclusion: There are differences in the expression profiles of microRNAs between the Chinese medicine treatment group and the western medicine control group before and after the treatment of NSTEMI, and the screened miRNAs may be the intervention targets for Chinese medicines of promoting blood flow and eliminating blood stasis.关键词：Non-ST segment elevation myocardial infarction;microRNA;salvianolate;therapeutic targets;bioinformatics
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发布时间：2024-01-04 - 摘要：Objective: To investigate the correlation between blood stasis syndrome (BBS) and the interleukin-6 (IL-6) gene promoter methylation state in the unstable angina (UA) patients. Method: Six BBS in patients or out patients from the Department of Cardiology, Guang'anmen Hospital of China Academy of Chinese Medical Sciences were recruited from February to May in 2015 as the test group, while 6 non BBS in patients or out patients were recruited as control group. The IL-6 gene promoter methylation states were detected by bisulfite and compared between two groups to analyze the correlation between BBS and gene promoter methylation state. Result: The methylation degree of BBS group was potentially higher than that of non BBS group on 7 sites from -1 118 to -826 bp before the transcription initiation site of IL-6, but with no statistically difference between the two groups; and there was no significant difference in the methylation degree on 4 sites from -1 471 to -1 184 bp before the transcription initiation site of IL-6 between two groups. Conclusion: It could be inferred that the methylation states of BBS group were potentially higher than those of non BBS group on IL-6 promoter, in part reflecting the essence of BBS.关键词：coronary heart disease;Unstable Angina;blood stasis syndrome;DNA methylation;epigenetics;bisulfite method
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发布时间：2024-01-04 - 摘要：Objective: To identify the differentially expressed lncRNA, miRNA and mRNA levels related to coronary heart disease (CHD) blood stasis syndrome, construct the interaction network, and explore the material foundation and mechanism of blood stasis syndrome from the perspective of transcriptomics. Method: The expression levels of lncRNA, miRNA and mRNA in CHD blood stasis syndrome group, non-blood stasis syndrome group and healthy group were detected via high-throughput sequencing technology, and the differentially expressed genes related to CHD blood stasis syndrome were identified by using crosslinking analysis. Functional and pathway enrichment was performed for the obtained differentially expressed genes, and then the interaction network was constructed by Pearson correlation analysis and starBase prediction. Key nodes of interaction network were screened out by tropical analysis. Another cohort was enrolled to verify the key nodes in interaction network with Real-time PCR. Result: The 39 lncRNAs, 229 miRNAs and 221 mRNAs were closely related to CHD blood stasis syndrome. Functional and pathway analysis results showed that the differentially expressed genes were majorly correlated to immune and inflammation. A total of 9 lncRNAs (all down-regulated), 31 mRNAs (11 up-regulated and 20 down-regulated) and 24 miRNAs (14 up-regulated and 10 down-regulated) constituted the regulation network, including 76 intergenic relationships. CTA-384D8.35, CTB-114C7.4, RP11-567M16.6 and hsa-miR-3158-3p were the key nodes in CHD blood stasis syndrome interaction network, which was verified by Real-time PCR. Conclusion: CHD blood stasis syndrome has specific lncRNA, miRNA and mRNA expression profiles, closed related to immunity and inflammation; gene-gene interactions exist among differentially expressed genes, and CHD blood stasis syndrome related lncRNA-miRNA-mRNA interaction network can be constructed on this basis.关键词：coronary heart disease (CHD) blood stasis syndrome;lncRNA;miRNA;high-throughput sequencing;transcriptomics
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发布时间：2024-01-04 - 摘要：Objective: To investigate the differences of volatile constituents between the raw and processed products of Viticis Fructus,and discuss processing mechanism of this herb. Method: The comparative analysis was performed with GC-MS on the composition of volatile compounds from 21 batches of Viticis Fructus before and after processing.The similarity evaluation software was applied to evaluate the similarities of raw and processed samples within each group.Samples of crude Viticis Fructus and their corresponding processed samples with similarities>0.922 were chosen to conduct the group comparison.Principal component analysis (PCA) and other chemometric methods were applied to study on the chemical recognition,and find the main difference compounds between the two groups. Result: There were no significant differences on chemical composition in raw and processed samples,and 60 compounds among them were identified.PCA showed that there were a certain difference between the raw and processed samples of Viticis Fructus,and 12 compounds had significant changes,which were found between them with the help of partial least squares discriminant analysis (PLS-DA). Conclusion: Processing can affect the content of volatile compounds in Viticis Fructus,but it has no effect on the type of ingredients,which will provide reference for the quality control of Viticis Fructus.关键词：Viticis Fructus;processing;volatile components;chemometrics;8-propoxy cedrane;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To prepare Coptidis Rhizoma pieces standard decoction and establish its quality standard. Method: According to the standard of Chinese herbal medicine decoction preparation principle,Coptidis Rhizoma pieces standard decoction was prepared,the transfer rates of epiberberine,coptisine hydrochloride,palmatine hydrochloride and berberine hydrochloride and the extract rate were calculated.The quality standard of this standard decoction was also established. Result: The transfer rates of epiberberine,coptisine hydrochloride,palmatine hydrochloride and berberine hydrochloride were 79.3%-111.9%,54.6%-76.2%,45.7%-70.7% and 43.5%-64.4%,respectively;and the extract rate was 17.1%-22.3%.Moreover,7 common peaks were determined in 14 batches of Coptidis Rhizoma pieces standard decoction.The similarities of samples were all higher than 0.999. Conclusion: The method displays good precision,stability and repeatability in fingerprint analysis,which is suitable for the quality evaluation of Coptidis Rhizoma pieces standard decoction.关键词：Coptidis Rhizoma;standard decoction;pieces;fingerprint;epiberberine;coptisine;palmatine;berberine
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发布时间：2024-01-04 - 摘要：Objective: To compare the different effect of Rehmanniae Radix Praeparata processed by different methods on the chemical damage anemia in mice as model of blood deficiency. Method: Blood deficiency model were induced by phenylhydrazine hydrochloride and cyclophosphamide.The experimental animals were randomly divided into blank group,model group,Ejiao blood enriching oral liquid group,traditional wine stewing group and origin processing integration group.The administration volume was 0.02 mL·g-1,and dose of the latter three was 10 mL·kg-1,3.75,3.75 g·kg-1,respectively.Enriching blood effect of Rehmanniae Radix Praeparata processed by different methods was evaluated by taking hemogram,level of erythropoietin(EPO) in serum and organ index as indexes. Result: Compared with the model group,red blood cells (RBC) of each administration group increased significantly (P<0.01);white blood cells (WBC) and hemoglobin (HB) of origin processing integration group increased significantly (P<0.01),EPO was gradually restored to normal level and significantly decreased (P<0.01);WBC was increased in the traditional wine stewing group (P<0.05),while EPO (P<0.05) returned to normal levels;Thymus index of origin processing integration group was significantly increased (P<0.01) and the parameter in traditional wine stewing group was increased (P<0.05).Compared with the blank group,these indexes of model group were significantly different.Compared with the Ejiao blood enriching oral liquid group,HB (P<0.05) was significantly increased in the origin processing integration group.Compared with the traditional wine stewing group,HB of the origin processing integration group was significantly increased (P<0.01). Conclusion: Rehmanniae Radix Preparata processed by different methods has a certain blood enriching effect.Among them,the origin processing integration group is better than the traditional wine stewing group.关键词：Rehmanniae Radix Praeparata;enriching blood;blood deficiency;processing technology;integrative processing
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of potassium nutrition levels on contents of ligustilide, senkyunolide A, senkyunolide H,senkyunolide I, coniferylferulate, n-butylidenephthalide and levistilide A from Angelicae Sinensis Radix in different growth periods, and provide a theoretical basis for reasonably formulating theapplication scheme of potassium fertilizer in the standardized production of Angelicae Sinensis Radix. Method: In field cultivation experiment conditions, the effects of K2O of 0 kg·hm-2 (K0), 150 kg·hm-2 (K1), 300 kg·hm-2 (K2), 450 kg·hm-2 (K3), 600 kg·hm-2 (K4) and 750 kg·hm-2 (K5) on phthalides in Angelicae Sinensis Radix were studied. The contents of the above 7 phthalides components during different growth periods in roots of Angelicae Sinensis Radix were determined by HPLC method. Result: Potassium application did not change the variation trend of phthalides constituents along with the growth period in roots of Angelicae Sinensis Radix, but different volumes of potassium fertilizer application had significant effects on the contents of phthalides components(P<0.05). Under the experimental conditions, K2O 450 kg·hm-2 had the best effect on the contents of phthalides components in Angelicae Sinensis Radix. Conclusion: The appropriate level of potassium fertilizer can increase the contents of phthalides constituents in roots of Angelicae Sinensis Radix, but did not change the overall dynamic changes trend of contents of phthalides components in the roots of Angelicae Sinensis Radix.关键词：potassium;Angelicae Sinensis Radix;phthalides;dynamic changes
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发布时间：2024-01-04 - 摘要：Objective: Total phenolics, total flavonoids, polysaccharide, arecoline, arecaidine and guvacine were selected as target compositions, and meanwhile, ultraviolet spectrophotometry and ultra-performance liquid chromatography-diode array detector (UPLC-PDA) were used and combined with the changes of appearance traits and moisture content to investigate the effects of different storage environments and packaging materials on the quality of Arecae Semen. Method: The methods including folin-phenol method,aluminum trichloride coloring method and phenol-sulfuric acid method were adopted, and the contents of total phenolics, total flavonoids and polysaccharides were detected with a wavelength of 775, 510,489 nm, respectively. The samples were extracted by ultrasonic approach with trichloromethane-methanol (4:1) as extracted solvent. Then, the analysis was performed on a BEH HILIC column (2.1 mm×100 mm, 2.7 μm) with acetonitrile-10 mmol·L-1 ammonium acetate solution as the mobile phase for gradient elution. Finally, the contents of three alkaloids in Arecae Semen were determined at 215 nm by the established UPLC-PDA method. Result: After storage for 1 year under different storage conditions and packaging materials, areca appearance color was deepened, moisture content was increased, and the contents of total phenolics, total flavonoids, polysaccharide and alkaloids were reduced with the increase of storage time. In addition, their contents were significantly affected by high temperature and high humidity (P<0.01),normal temperature and humidity (P<0.01) and packaging materials of woven bags (P<0.01) and kraft paper (P<0.01), while low temperature and humidity (P<0.05) and self-sealed bags (P<0.05) showed little effect on the content of target compositions. Conclusion: Arecae Semen is recommended to be stored in self-sealed bags under low temperature and low humidity conditions for longer storage time and quality.关键词：Arecae Semen;storage environments;packaging materials;total phenolics;total flavonoids;polysaccharide;alkaloids
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发布时间：2024-01-04 - 摘要：Objective: To discuss the traditional Chinese medicine (TCM) name, Chinese name of original plant and scientific name in the 2015 edition Chinese Pharmacopoeia, as well as the Chinese name and scientific name of the original plant in Flora of China. Method: The TCM names, Chinese names of their original plants and scientific names in the 2015 edition Chinese Pharmacopoeia, as well as the Chinese names and scientific names of the original plants in Flora of China were analyzed and summarized. Result: There was confusion and cross confusion between TCM names and Chinese names of original plants in the 2015 edition Chinese Pharmacopoeia and those in Flora of China, and their scientific names also might be different. First, the Chinese names of original plants for some Chinese herbs in the Chinese Pharmacopoeia were inconsistent with the Chinese names of the plants in Flora of China. Second, the scientific names of original plants for some Chinese herbs in the Chinese Pharmacopoeia were inconsistent with those in Flora of China. Third, the Chinese names and scientific names of original plants for some Chinese herbs in the 2015 edition Chinese Pharmacopoeia were inconsistent with those in Flora of China. Forth, some TCM names in the 2015 edition Chinese Pharmacopoeia were the same with the Chinese names of other plants in Flora of China. Conclusion: TCM names, Chinese names of the original plants, and scientific names in Chinese Pharmacopoeia shall be consistent with those in Flora of China, helpful for people to carry out planting, purchasing, sales, researching and production of Chinese herbs, and it will also benefit TCM in international communication activities.关键词：Chinese pharmacopoeia;Flora of China;traditional Chinese medicine name;scientific name;Chinese name of original plant
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发布时间：2024-01-04 - 摘要：Objective: To analyze and identify Alumen,calcined Alumen and their counterfeits by using X-Ray diffraction (XRD),Near Infrared (NIR) and Raman spectroscopy technique. Method: Nine batches of Alumen and 5 batches of calcined Alumen were purchased from the market, and another 2 batches of calcined Alumen were prepared by ourselves. Their XRD, NIR and Raman spectra were collected,and their spectral characteristics and differences were analyzed. Result: There were significant differences between Alumen and its counterfeit Ammonium alum in XRD,NIR and Raman spectra. In XRD patterns, Ammonium alum had a peak at 14.56°, while Alumen had no such peak. In NIR spectra, Ammonium alum had a characteristic peak at 4 650 cm-1,while Alumen had no such peak. In Raman spectra, the strongest peak of Alumen at 990 cm-1 was split to produce a moderate intensity peak at 974 cm-1, while Ammonium alum had no such peak at 974 cm-1. The above differences could be used for the rapid identification of Alumen and its counterfeit Ammonium alum. Besides,calcined Alumen had 9 characteristic peaks (2θ=11.18°, 21.88°, 24.58°, 31.42°, 33.76°, 38.25°, 39.94°, 50.24ånd 50.99°) in XRD patterns and 2 absorption peaks (7 000 cm-1 and 5 150 cm-1) in NIR spectra, which could be used as the characteristic peaksto identify the calcined Alumen and its counterfeit. Conclusion: XRD, NIR and Raman spectral characteristicscould be used for the rapid identification of Alumen,calcined Alumen and their counterfeits. NIR combined with Raman spectra could provide a rapid and accurate method for the quality control and rapid identification of Alumen and its processed products.关键词：Alumen;calcined Alumen;rapid identification;XRD;NIR;Raman spectroscopy
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发布时间：2024-01-04 - 摘要：Objective: To analyze the main chemical components ingypenosidesextracts with UPLC-ESI-Orbitrap-MS technology. Method: Thermo Q Exactivequadrupolerod-electrostatic field orbitrap high resolution mass spectrometry-MS system was used and the chromatography separation was performed on Hypersil Gold C18 column (2.1 mm×150 mm, 1.9 μm) with 0.1% formic acid solution (A)-acetonitrile (B) as the mobile phase for gradient elution (0-2 min, 5%B; 2-15 min, 5%-95%B; 15-17 min, 95%B). The flow rate was set at 0.3 mL·min-1 and the column temperature was 40℃.Electrospray ionization (ESI) source was applied and operated in Full Scan/Targeted-dd MS2/negative ion mode. Result: Eight gypenosides were identified based on the reference compounds, high resolution mass number and comparison between experimental data and published data. These compounds included gypenoside Ⅲ,Ⅳ,Ⅷ,Ⅻ, Rg3, Rb2, Rc, and Rf. Conclusion: The established method would be used to qualitatively and rapidly compare the quality of extract from Gynostemma pentaphyllum on sale.关键词：Gynostemma pentaphyllum;total saponins;UPLC-ESI-Orbitrap/MS
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发布时间：2024-01-04 - 摘要：Objective: To identify and determine the main chemical constituents in Lonicerae Japonicae Flos by RRLC-DAD-ESI-Q-TOF-MS, and establish the quantitative fingerprint of Lonicerae Japonicae Flos of Shandong province. Method: The rapid analysis of Lonicerae Japonicae Flos extract was carried out by RRLC-DAD-ESI-Q-TOF-MS. The chemical formula of Lonicerae Japonicae Flos was inferred by using the exact molecular weight and secondary mass spectrometry information. The chemical compositions of Lonicerae japonicae flos were identified by data base combined with literature reviewing. The contents of main chemical components in Lonicerae Japonicae Flos from Shandong and Henan as well as Lonicerae Flos were determined by using RRLC-DAD method, and the quantitative fingerprint was established on this basis for the quality evaluation of different samples. Result: The 51 chromatographic peaks were detected and 32 chemical constituents were identified from Lonicerae Japonicae Flos. The contents of 10 major chemical contents (loganic acid,chlorogenic acid, caffeic acid, loganin,secoxyloganin, rutin,hyperoside,luteoloside,isochlorogenic acid A and isochlorogenic acid C) in Lonicerae Japonicae Flos from Shandong and Henan as well as Lonicerae Flos were determined and compared. The differentiation of different species of Lonicerae Japonicae Flos and Lonicerae Flos was achieved by RRLC fingerprint combined with similarity analysis, and the result wasconsistent with the content determination result. Conclusion: RRLC-DAD-ESI-Q-TOF-MS technique is fast and accurate, providing accurate quality information for compounds and their fragment ions. It can quickly and accurately identify and determine the chemical compositions of Lonicerae Japonicae Flos, and the multiple indicator quantitative fingerprint based on this study could provide technology support for the quality evaluation of Lonicerae Japonicae Flos.关键词：Lonicerae Japonicae Flos;RRLC-DAD-ESI-Q-TOF-MS;organic acids;flavonoids;iridoids;multiple indicator quantified fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To identify the chemical constituents of Kuntai capsule by high performance liquid chromatography with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Method: The chemical components were analyzed and identified by using LC-ESI-MS/MS. The liquid chromatography separation was carried out on C18 column (4.6 mm×150 mm, 5 μm) with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1 and column temperature was 30℃. The mass spectra were obtained in positive ion mode with electrospray ionization. The mass range recorder was set between m/z 50-1 500.The molecular weight was determined according to the molecular ion peaks. The compound structures were elucidated by comparing the data with literature data,including main ion peaks, UV spectrum and HPLC retention time information. Result: The 21 compounds were identified from Kuntai capsule, mainly including flavones and alkaloids. The flavones, such as baicalin, wogonoside, baicalein and wogonin, were identified from Scutellaria baicalensis. The alkaloids, such as palmatine, berberine, coptisine, jatrorrhizine, columbarine and magnoflorine, were identified from Coptis chinensis; paeoniflorin came from Paeonia lactiflora. Conclusion: The method is simple and rapid for identifying the main constituents of Kuntai capsule. This paper has laid a foundation for further studying and testing the quality of Kuntai capsule.关键词：Kuntai capsule;chemical constituent;alkaloid;flavonoid;HPLC-ESI-MS
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents from caulis of Lonicera alberti and establish an HPLC method for simultaneously determining the contents of chlorogenic acid, 3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid in different pharmaceutical parts of L. alberti. Method: Chemical constituents were isolated and purified by various chromatographic techniques such as silica gel, reversed-phase silica gel, macroporous resin, and Sephadex LH-20 column chromatography. Their structures were elucidated by physicochemical properties and spectroscopic data. The chromatographic conditions were as follows:Phenomenex C18 column (4.6 mm×250 mm, 5 μm), with acetonitrile (A) and 0.2% phosphoric acid solution (B) as the mobile phase for gradient elution (0-13 min, 12%A; 13-25 min, 12%-22%A; 25-45 min, 22%A). The flow rate was 1.0 mL·min-1, detection wavelength at 327 nm, column temperature at 30℃,and injection volume at 10 μL. Result: Ten compounds were separated from L. alberti, and identified as 3,7-dimethylquercetin (1), 3,3'-dimethylquercetin (2), quercetin (3), ursolic acid (4), loganin (5), chlorogenic acid (6), quercetin 5-O-β-D-glucoside (7), 3,4-O-dicaffeoylquinic acid (8), 3,5-O-dicaffeoylquinic acid (9) and 4,5-O-dicaffeoylquinic acid (10). Compounds 6, 9 and 10 achieved baseline separation and showed good linearity, and the average recoveries were 100.15%, 99.68% and 99.52% respectively. The content of three compounds from L. alberti flower(3.99%, 1.77%,2.33%) was more than that of leaves (2.62%, 1.73%, 0.90%) and caulis (0.74%,0.25%, 0.15%). Conclusion: Compounds 1, 2 and 7 were isolated from Lonicera genus for the first time, and other compounds were found from this plant for the first time. The content determination method was accurate, reliable and convenient, so it could be used for content determination of chlorogenic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in L. alberti.关键词：Lonicera genus;Lonicera alberti;3,7-dimethylquercetin;3,3'-dimethylquercetin;quercetin 5-O-β-D-glucoside
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the mature fruits of Solanum muicatum. Method: S. muicatum dry fruits were heated and extracted by using 95% ethanol, and then were extracted with petroleum ether, ethyl acetate and n -butanol in turn. Ethyl acetate extract fraction of S. muicatum 95% ethanol extract was isolated and purified by using chromatography on silica gel, Sephadex LH-20 column chromatography and preparative HPLC methods. The structures of the compounds were then identified by analysis of NMR and spectroscopic data by comparison with those reported on the literature. Result: Seven compounds were isolated from 95% ethanol andthey were identified as solasonine(1),solamargine(2),solasodine(3),quercetin(4),naringenin(5),ferulic acid(6),and ursone(7). Conclusion: All of the compounds were obtained from this plant for the first time, providing certain chemical basis and foundation for the comprehensive development and the search for natural plant anti-inflammatory and antitumor active substances关键词：Solanum muicatum;mature fruits;nuclear magnetic resonance;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To establish the UPLC fingerprint of Chenxiang Huaqiwan for providing a reference for evaluation of the overall quality of this preparation. Method: The separation was performed on Phenomenex Kinetex C18 column (4.6 mm×100 mm,2.7 μm) with mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution at a flow rate of 0.8 mL·min-1,wavelength switching technology was used (0-3 min,285 nm;3-20 min,210 nm;20-24 min,254 nm;24-42 min,210 nm).According to the similarity evaluation,the chemometrics method was used to evaluate the quality of Chenxiang Huaqiwan. Result: Fingerprints of 20 batches of Chenxiang Huaqiwan were established,there were 27 common peaks in the fingerprints and 12 common peaks were identified by reference substances,fingerprints similarity of samples were over 0.98.The samples were classified into three groups by hierarchical cluster analysis combined with principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA),seven components were the main markers that cause differences in the different batches of samples. Conclusion: Establishment of fingerprint and application of chemical pattern recognition can provide more comprehensive reference for the quality control of Chenxiang Huaqiwan.The established UPLC fingerprint method is stable and feasible.关键词：Chenxiang Huaqiwan;fingerprint;cluster analysis;principal component analysis;orthogonal partial least squares-discriminant analysis
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of saponin component Ⅰ and Ⅱ in red Panacis Quinquefolii Radix on 17 neurochemicals in rat cerebral cortex and hippocampus. Method: Saponin component Ⅰ and Ⅱ were prepared from total saponins of red Panacis Quinquefolii Radix by D101 macroporous adsorption resin.The composition of saponins in component Ⅰ and Ⅱ were detected by UPLC-LIT-MS.The contents of 17 neurochemicals in rat cerebral cortex and hippocampus were determined by UPLC-QQQ-MS. Result: Compared with the blank group, the contents of glutamic acid,aspartic acid,taurine,acetylcholine,tyrosine,dopamine and norepinephrine in rat cerebral cortex and hippocampus increased after administration of saponin component Ⅰ;while the contents of γ-aminobutyric acid,tryptophan,5-hydroxytryptamine,5-hydroxyindoleacetic acid,melatonin,serine,glycine and histamine increased after administration of saponin component Ⅱ;and the trends were positively related to dose.Through analyzing the relationship of saponin composition with content variation of neurochemicals,saponin component Ⅰ could increase the contents of excitatory neurochemicals in rat cerebral cortex and hippocampus,while saponin component Ⅱ were shown to improve the inhibitory neurochemicals contents in rat cerebral cortex and hippocampus. Conclusion: The saponins in red Panacis Quinquefolii Radix has neural activity.The polarity of saponins is related to their effects on excitatory and inhibitory neurochemicals.关键词：Panacis Quinquefolii Radix;red Panacis Quinquefolii Radix;saponins;components;neurochemicals;glutamic acid;5-hydroxytryptamine
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发布时间：2024-01-04 - 摘要：Objective: Polygala saponins in the 60% ethanol extract of Kaixinsan (KXS-60% E) were identified,and the polygala saponins as well as their metabolites were analyzed in rat plasma after oral administration of KXS-60% E. Method: Ion fragments data of polygala saponins in KXS-60% E was identified by UPLC-TOF-MS.Similarly,the polygala saponins and their metabolites were also identified in rat plasma after oral administration of KXS-60% E with the ion fragments data,MassLynx v4.1 software and databases in the literatures. Result: A total of 14 polygala saponins which were mainly triterpene saponins,were identified in KXS-60% E in vitro.Moreover,5 polygala saponins as well as 4 metabolites were identified in rat plasma. Conclusion: Five polygala saponins as well as four metabolites may be the potential bioactive compounds and direct pharmcodynamic basis of KXS-60% E.Further study on these constituents may find out the effective compounds in KXS-60% E and clarify the mechanism on treatment of Alzheimer's disease.关键词：Kaixinsan;serum pharmacochemistry;Polygalae Radix;saponins;metabolites;ethanol extract;onjisaponin B
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发布时间：2024-01-04 - 摘要：Objective: To study on the pharmacokinetic profiles of evodiamine (EVO) and rutaecarpine (RUT) from Euodiae Fructus powder dressing on the foot sole of Japanese big-ear rabbits with vinegar. Method: The Euodiae Fructus powder (1 g·kg-1) was dressed on the foot sole of male Japanese big-ear rabbits with vinegar in 12 h,the blood was taken from the hearts at different time points (0,1,2,3,4,5,6,8,10,12,24 h) after administration.The plasma samples were pretreated by protein precipitation with acetonitrile,and the concentrations of EVO and RUT in rabbit whole blood were determined by LC-MS,taking Phenomenex Gemini C18 column (4.6 mm×150 mm,5 μm) with mobile phase of acetonitrile-5 mmol·L-1 ammonium formate aqueous solution (62:38),flow rate was 0.4 mL·min-1,column temperature was 25℃.Tryptanthrin (TRY) was adopted as the internal standard,electrospray ionization (ESI) was applied and operated in positive ionmode,quantification was performed by multiple reaction monitoring (MRM).The main pharmacokinetic parameters were calculated by PKSolver 2.0 according to non-compartment model. Result: The t1/2 of EVO and RUT were(18.55±7.98),(20.15±1.06) h,Tmax were 5,4 h,Cmax were (4.68±0.34),(9.76±1.05) μg·L-1,AUC0-t were (58.18±5.46),(155.38±13.91) μg·L-1·h,AUC0-∞ were (101.46±23.34),(300.32±36.22) μg·L-1·h,MRT0-∞ were (27.63±9.34),(31.25±1.47) h. Conclusion: EVO and RUT can be absorbed into the blood through the skin of Japanese big-ear rabbits,and the drug concentration can be maintained within 24 h after withdrawal,which provides the experimental basis for point-application of Euodiae Fructus.关键词：Euodiae Fructus;evodiamine;rutaecarpine;administration at foot sole;drug metabolism;tryptanthrin
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Liandou Qingmai recipe on atherosclerosis(AS) plaques of Japanese rabbits and poly adenosine diphosphate ribose polymerase-1 (PARP-1). Method: Totally 40 healthy male Japanese white rabbits were randomly divided into two groups. Among them, 8 rabbits were randomly selected into blank group (Bg). The remaining 32 rabbits, according to the literature method, were selected to establish AS plaque model through feeding high fat diet and intravenous injection with bovine serum albumin. Finally, 27 rabbits were successfully modeled and randomly divided into 3 groups:high-fat group included 11 rats (2 rats died during the experiment, HFg), Liandou Qingmai recipe treatment group (LTg) included 8 rats, and simvastatin treatment group (STg) included 8 rats, which were given high-fat diet, Liandou Qingman recipe drug diet, simvastatin diet respectively, with the doses of 1 mg·kg-1·d-1 for simvastatin, and 3.7 g·kg-1·d-1 for Liandou Qingman recipe. After 8 weeks, AS plaques, PARP-1 positive expression, Toll like receptor-4 (TLR-4) mRNA expression, nuclear factor-kappa B (NF-κB), serum interleukin-6 (IL-6) were detected. Result: The Bg did not appear AS plaque. The LTg's AS plaque significantly lower than that of the high-fat group. Compared with the Bg, the LTg's AS plaque area, aortic and tissue expressions of PARP-1 and TL-4 mRNA, levels of NF-κB and IL-6 increased. But compared with the HFg, the LTg's figures decreased. The differences were statistically significant (P<0.01). Conclusion: Liandou Qingmai recipe can inhibit the proliferation of AS plaque; the mechanism may be related to inhibition of the expression of PARP-1 and other inflammatory reactions.关键词：atherosclerosis;Liandou Qingmai recipe;poly adenosine diphosphate ribose polymerase-1;Toll like receptor-4;nuclear factor-kappa B;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: To establish the vitro diabetic nephropathy model with high-glucose induced-rat glomerular mesangial cells to detect the inhibitory effect of ethyl acetate extract of Coreopsis tinctoria (AC) on cell proliferation and fibrosis factor expression. Method: High glucose-induced rat glomerular mesangial cells were treated with AC. The cells were divided into six groups:normal group, high-glucose-induced model group, high-glucose+AC groups, with concentrations of 25, 50,100,150 mg·L-1. MTT and cell counting kit 8 (CCK8) were used to test the inhibitory effect of AC on cell proliferation. Real-time PCR and Western blot were used to test the inhibitory effect of AC on transforming growth factor-β1 (TGF-β1), Collagen Ⅳ, fibronectin (FN) mRNA and protein expressions. Result: AC with concentrations of 25, 50, 100, 150 mg·L-1 significantly inhibited the proliferation of glomerular mesangial cells of high-glucose-induced rats (P<0.01). AC with concentrations of 25, 100, 150 mg·L-1 significantly inhibited TGF-β1 mRNA and protein expressions (P<0.01). AC with concentrations of 100, 150 mg·L-1 significantly inhibited Collagen IV mRNA and protein expressions (P<0.01). AC with concentrations of 50, 100, 150 mg·L-1 significantly inhibited FN mRNA and protein expressions (P<0.01). Conclusion: AC prevents the diabetic nephropathy renal fibrosis by suppressing proliferation of rat glomerular mesangial cells and fibrosis factor expression.关键词：ethyl acetate extract of Coreopsis tinctoria;rat glomerular mesangial;cell proliferation;fibrosis factor expression
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Panax Notoginseng saponins (PNS) on endoplasmic reticulum stress factors of glucose regulated protein 78 ku (GRP78), phosphorylated eukaryotic translation initiation factor (p-eIF2α), phosphorylated PKR like endoplasmic reticulum regulating kinase (p-PERK) and inflammatory factors of phosphorylated nuclear factor-Kappa B (p-NF-κB), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta(IL-1β) in liver tissues of natural aging rats, and explore the protective mechanism of PNS on the endoplasmic reticulum stress mediated inflammatory response in aging rats' livers. Method: The 50 SPF SD rats were randomly divided into youth group (9 months), natural aging groups (24 months), low-, medium-and high-dose PNS groups, n=10 in each group. PNS was given at 10, 30, 60 mg·kg-1 respectively in PNS groups from 18 months old to 24 months old, 6 days a week by intragastric administration. The rats were executed 12 hours after the last medication(food fasting, but water was given), and their livers were taken out immediately and storedat -80℃refrigerator aftertreatment by liquid nitrogen. Hematoxylin-eosin (HE) staining was used to observe the morphological changes of liver tissues in different groups, and Western blot was used to detect the protein changes of endoplasmic reticulum stress related factors GRP78, p-eIF2α, p-PERK and inflammatory related factors p-NF-κB, TNF-α, IL-1β in liver tissues. Result: As compared with the young rats' livers, the contents of GRP78, p-eIF2α, p-PERK, p-NF-κB, TNF-α, and IL-1β in the natural aging rats' livers were increased (P<0.01); the liver changes were not obvious in low-dose PNS group;the protein expression levels of endoplasmic reticulum stress related factors and inflammatory related factorsin livers were decreased significantly in middle-and high-dose PNS groups (P<0.01, P<0.05). Conclusion: PNS can protect the naturally aging rats from inflammation induced by endoplasmic reticulum stress related factors and inflammatory related factors in livers.关键词：Panax Notoginseng saponins(PNS);aging;liver;endoplasmic reticulum stress;inflammation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect and mechanism of baicalin in alleviating Helicobacter pylori(Hp)-induced human gastric epithelial GES-1 cells injury. Method: baicalin (15, 30, 60 mg·L-1) or a specific inhibitor of p38 MAPK pathway SB203580 20 μmol·L-1 was added into Hp-infected GES-1 cells. Proliferation and apoptosis, levels of interleukin-1β (IL-1β) and IL-8, lactic dehydrogenase (LDH) activities, protein expressions of B-cell lymphoma-2(Bcl-2) associated X protein(Bax), Bcl-2, p-p38 MAPK and total-p38 MAPK(T-p38 MAPK) in GES-1 cells were determined by MTT assay, flow cytometry, ELISA, colorimetry and Western blot, respectively. Result: Compared with control group, Hp significantly inhibited proliferation of GES-1 cells, increased apoptosis, LDH activities, IL-1β and IL-8 levels, Bax and p-p38 MAPK expressions, but decreased Bcl-2 expression in GES-1 cells (P<0.01). Compared with Hp-infected GES-1 cells, medium-dose and high-dose baicalin significantly increased proliferation of GES-1 cells, decreased apoptosis, LDH activities, IL-1β and IL-8 levels, Bax and p-p38 MAPK expressions, but increased Bcl-2 expression in GES-1 cells (P<0.01). Compared with the high-dose baicalin group, SB203580 significantly increased proliferation of GES-1 cells, decreased apoptosis, LDH activities, IL-1β and IL-8 levels, Bax and p-p38 MAPK expressions, but increased Bcl-2 expression in GES-1 cells (P<0.05, P<0.01). Conclusion: baicalin alleviated Hp-induced human gastric epithelial GES-1 cells injury through anti-inflammation, proliferation promotion and anti-apoptosis. The mechanism may be correlated with the inhibition of p38 MAPK signaling pathway.关键词：baicalin;helicobacter pylori;p38 mitogen-activated protein kinase;human gastric epithelial GES-1 cells;inflammation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Buyang Huanwutang serum on proliferation and cycle of bone mesenchymal stem cells (BMSCs). Method: Buyang Huanwutang (6.5,13,26 g·kg-1) and equivalent distilled water were administered (ig) to SD rats for a week, so as to prepare three kinds of Buyang Huanwutang serum and the blank serum. BMSCs cells were divided into Buyang Huanwutang 6.5 g·kg-1 group (cultured with 10% serum from Buyang Huanwutang 6.5 g·kg-1), Buyang Huanwutang 13 g·kg-1 group (cultured with 10% serum from Buyang Huanwutang 13 g·kg-1), Buyang Huanwutang 26 g·kg-1 group (cultured with 10% serum from Buyang Huanwutang 26 g·kg-1), and blank group (cultured with 10% serum from distilled water). Cell proliferative activity, cell cycle and protein expressions of phosphatidylinositol 3-kinase (PI3K), phosphorylation serine/threonine kinase (p-Akt) (Ser473) were detected by MTT, flow cytometry and western blot respectively. Result: BMSCs proliferation was increased in Buyang Huanwutang 13, 26 g·kg-1 groups after being cultured for 48,72 h, compared with the blank group (P<0.05). After being cultured with the three kinds of Buyang Huanwutang serum for 48 h, S-phase cell rate in BMSCs was significantly higher than that in blank group (P<0.05). Protein expressions of PI3K, p-Akt(Ser473) were up-regulated in BMSCs treated with serum from Buyang Huanwutang 13,26 g·kg-1 for 48 h, compared with blank group (P<0.01). Conclusion: Serum from Buyang Huanwutang 13,26 g·kg-1 may promote BMSCs into the S phase, and enhance the proliferation of BMSCs by activating PI3K/Akt signaling pathway.关键词：Buyang Huanwutang;bone mesenchymal stem cell;proliferation;cell cycle;phosphatidylinositol 3-kinase/serine/threonine kinase
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of P6(<6 kDa) on activation, proliferation and hepatofibrosis-related genes in rat hepatic stellate cell line HSC-T6 induced by transforming growth factor-β (TGF-β), in order to explore the mechanism of anti-hepatofibrosis effect. Method: Dialysis was applied to get P6. Cell counting Kit 8 (CCK8) was performed to evaluate cell viability and P6 effect on HSC-T6 proliferation. The mRNA expressions of α-smooth muscle actin (α-SMA), collagen type I (Col I), tissue inhibitor of metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-2 (MMP-2) were determined by Real-time PCR. Protein expressions of α-SMA and Col I were assessed by Western blot. Result: Compared with control group, P6 did not significantly affect cell viability, but suppressed TGF-β-induced HSC-T6 proliferation (P<0.05). Compared with TGF-β group, mRNA expressions of α-SMA, Col I and TIMP-1 were significantly decreased by P6 in TGF-β-induced HSC-T6, while MMP-2 mRNA expression was markedly increased (P<0.05, P<0.01), α-SMA and Col I protein expressions were also decreased obviously. Conclusion: These results demonstrated that P6 had an anti-hepatofibrosis effect by inhibiting HSC-T6 activation and proliferation induced by TGF-β, reducing production and accelerating degradation of ECM.关键词：Carapax trionycis;HSC-T6;hepatofibrosis;activation;mechanism
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Chinese traditional medicine(TCM) of' cleaning heat, cooling blood, disintoxication and removing blood stasis' combined with stem cell transplantation in treating the rats of acute liver failure, in order to to explore the effect on apoptosis of hepatocytes. Method: A total of 40 clean SD rats were randomly divided into the blank group (5 rats) and the model group (35 rats). The acute liver failure was induced by D-GalN (1.2 g·kg-1) and LPS (5 μg·kg-1) through intraperitoneal injection. At 12 hours after modelling, the living rats were randomly divided into the model group, the Compound Glycyrrhizin Tablet group, the Yinchen Siling granule group, the stem cell transplantation group and the combined treatment group (stem cell transplantation combined with TCM). The rats in the combined treatment group and the stem cell group were injected with BMSCs through portal vein, and the rats in the other groups were injected with physiological saline. The combined treatment group and the Yinchen Siling granule group were given by gavage twice a day with tradition Chinese drugs named Yinchen Siling granule (1.5 g·kg-1), the compound glycyrrhizin tablet group was given compound glycyrrhizin tablet (7.5 mg·kg-1) twice a day, and the rats in the other groups were provided with the equal volume of physiological saline until the rats were killed. Rat blood samples and liver tissues were collected in 120 hours, and alanine aminotransferase (ALT), aspartic aminotransferase (AST), total bilirubin (TIBL) were determined to see whether liver function in each group was improved and the extent. The liver tissue pathological changes were observed by HE staining, the apoptosis index was detected by TUNEL staining, protein expressions of cysteine aspartate protease-3(Caspase-3) was determined by Western blot, and mRNA expressions of B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) were detected by Real-time PCR. Result: Liver function abnormality occurred in rats with acute hepatic failure. Compared with the model group, the positive control group, the Chinese medicine group and the stem cell transplantation group, the combined treatment group was statistically better, with decreases in AST, ALT and TBIL, hepatic inflammatory activity and apoptosis index (P<0.05). Caspase-3 in each group, except for the blank group, was significantly increased, and the combined treatment group was statistically superior to all of the other groups (P<0.05). mRNA expression of Bax in each group was gradually decreased, while Bcl-2 mRNA increased, whereas Bax mRNA expression of the combined group was statistically lower all the other groups, and Bcl-2 mRNA was higher than the other groups (P<0.05). Conclusion: The combined treatment of TCM of' cleaning heat, cooling blood, disintoxication and removing blood stasis' and BMSCs transplantation alleviates liver dysfunction, protects liver tissues and inhibits the hepatic apoptosis, and the mechanism may be related to down-regulation of the expression of Caspase-3 protein, and up-regulation of the expression of Bcl-2 mRNA and down-regulation of the expression of Bax mRNA, with a better effect than using either of them alone; and both are synergistic to the suppression of hepatic apoptosis.关键词：acute liver failure (ALF);Chinese traditional medicine of cleaning heat, disintoxication, cooling blood and removing blood stasis;bone marrow mesenchymal stem cells (BMSCs);apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of Pollen Pini on oxidative damage of HepG2 cells induced by H2O2. Method: HepG2 cells were divided into normal control group, H2O2 model group and intervention group. HepG2 cells of the intervention group were pretreated with different concentrations of Pollen Pini for 12 h. Then, to produce oxidative stress, the H2O2 model group and the intervention group were treated with 400 μmol·L-1 H2O2 to damage cells for 2 h. Cell viability was measured by methylthiazolyl tetrazolium (MTT) assay. To evaluate the protective effect of Pini Pollen on HepG2 cells, cell viability, intracellular reactive oxygen species (ROS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, lactate dehydrogenase (LDH) and malondialdehyde (MDA) contents were detected. In addition, the expressions of key genes nuclear factor-E2-related factor 2 (Nrf2), Kelch-like epichlorohydrin-associated protein 1 (Keap 1), heme oxygenase-1 (HO-1), glutamate-cysteine ligase (GCL) in antioxidant pathway were determined by western blot. Result: MTT results showed that, compared with normal control group, the cytotoxicity of Pollen Pini groups (80, 40, 20, 10, 5 mg·L-1) were non-toxic. The contents of ROS, LDH and MDA in the H2O2 model group were significantly increased (P<0.01), while the activities of SOD and GSH-Px were significantly depressed (P<0.01). Compared with the H2O2 model group, the contents of ROS, LDH and MDA in the intervention group were significantly reduced (P<0.05, P<0.01), whereas the activities of SOD and GSH-Px were significantly increased (P<0.05, P<0.01). Western blot results indicated that pollen pini up-regulated protein expressions of Nrf2, HO-1 and GCL, but down-regulated the expression of Keap1 protein. Conclusion: The 5-20 mg·L-1 of Pollen Pini can effectively protect 400 μmol·L-1 H2O2-induced oxidative stress damage in HepG2 cells. The mechanism may be related to regulation of the activity of SOD, GSH-Px activity, ROS, LDH and MDA levels, and protein expressions of key genes Nrf2, Keap 1, HO-1, and GCL in the antioxidant pathway.关键词：Pollen Pini;HepG2 cell;anti-oxidation;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-proliferation effect of homoisoflavonoids extracted from Polygonati Odorati Rhizoma on human lung cancer A549 cells. Method: Ethanol was used as the solvent to extract homoisoflavonoids from Polygonati Odorati Rhizoma; methylthiazolyldiphenyl-tetrazolium bromide (MTT) method was used to observe the proliferation inhibition rates of lung cancer A549 cells after being treated with homoisoflavonoids (12.5, 25, 50, 100 mg·L-1) for 6, 12, 24 h. Apoptotic rate and cell cycles were analyzed through flow cytometer (FCM). And Western blot analysis was used to detect the expressions of apoptosis-related (Caspase-3, the Bcl-2, Bak) and cell cycle-related (p-Cdc, Cdc 2, p38) protein. Result: Compared with normal group, A549 cells treated by homoisoflavonoids extracted from Polygonati Odorati Rhizoma were observed as typical apoptotic cells. The results of MTT and FCM showed that homoisoflavonoids could significantly inhibit tumor cell proliferation and induce apoptosis of A549 in a dose and time-dependent manner(P<0.05, P<0.01). And the cell number in G2/M period was increased after being treated with homoisoflavonoids for 12 h (P<0.05). Compared with normal group, the cells treated with 25, 50, 100 mg·L-1 homoisoflavonoids, Caspase-3 and Bak were up-regulated, while Bcl-2 was down-regulated significantly (P<0.01); p-Cdc 2 and p38 were up-regulated, while Cdc 2 was down-regulated (P<0.01). Conclusion: Homoisoflavonoids extracted from Polygonati Odorati Rhizoma can promote the apoptosis of A549 cells and increase the proportion of cells in G2/M. The antitumor mechanism of homoisoflavonoids is related to mitochondria-mediated apoptosis and p38 MAPK pathway.关键词：homoisoflavonoids extracted from Polygonati Odorati Rhizoma;A549 cells;cell cycle;Caspase-3;Bcl-2;Bak;Cdc 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Yisui Bushen formula in improving malignant lymphoma chemotherapy-related anemia and immune function. Method: Totally 110 cases of malignant lymphoma chemotherapy-related anemia patients treated in our Haematology Department from January 2010 to December 2015 were selected and randomly divided into treatment group and control group according to random number table method, with 55 cases in each group. The control group was given conventional chemotherapy, while the treatment group was given Yisui Bushen formula for oral treatment (1/d) in addition to conventional chemotherapy. One course of treatment was 15 days, and they received 2 courses of treatment. Blood routine examination was performed, and the quality of life, clinical curative effect and complications of the two groups were observed and compared. Result: The total effective rate of the control group was 69.09%, whereas the total effective rate of the treatment group was 89.09%, indicating a statistically significant difference (P<0.05). Compared with before treatment, hemoglobin (Hb), hematocrit (Hct), red blood cell (RBC) content of the two groups increased after treatment (P<0.05), while the recombinant human erythropoietin (EPO) content reduced, suggesting statistically significant differences (P<0.05). After treatment, scores of body, role, social, emotional, and other indicators were increased, indicating statistically significant differences (P<0.05). The effectiveness of treatment group was 87.80%, which was higher than 68.29% of control group (P<0.05). After treatment, the levels of CD3+, CD4+, CD4+/CD8+ and CD8+ cells in treatment group were significantly lower than those in control group (P<0.05). The incidence of adverse reactions of control group was 12.73%, whereas the incidence of adverse reactions of treatment group was 7.27%, indicating a statistically significant difference in adverse reactions between two groups (P<0.05). Conclusion: Yisui Bushen formula shows an obvious clinical effect in improving malignant lymphoma chemotherapy-related anemia, the quality of life and immune function, with good synergy and attenuation.关键词：Yisui Bushen formula;malignant lymphoma;chemotherapy-related anemia;quality of life;synergy and attenuation;immune function
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Xingnaojing injection in the treatment of acute cerebral hemorrhage, oxidative stress and serum inflammatory factors. Method: Totally 108 cases of acute cerebral hemorrhage patients treated in our hospital from January 2015 to January 2016 were selected as the study objects, and divided into two groups according to the random number table, with 54 in each group. The two groups of patients were given conventional treatment, and the observation group was treated with Xingnaojing injection. Clinical curative effect, changes of serum inflammatory factors and oxidative stress index level of two groups before and after the treatment were compared. Result: The total effective rate of observation group was 96.30%, which was significantly higher than 85.19% of control group (P<0.05). After the treatment, the National Institute of Health Stroke Scale (NIHSS) score of the two groups decreased significantly, while the Glasgow Coma scale (GCS) score significantly increased (P<0.05), indicating a better improvement effect in observation group than control group (P<0.05). After the treatment, the serum levels of interleukin (IL)-6, high-sensitivity C-reaction protein (hs-CRP) and tumor necrosis factor-α (TNF-α) were significantly lower than those before the treatment in two groups (P<0.05), and the levels of serum IL-6, hs-CRP and TNF-α in observation group were significantly lower than those in control group (P<0.05). After the treatment, the serum malondialdehyde (MDA) level of two groups decreased significantly, while the level of superoxide dismutase (SOD) increased significantly (P<0.05). The two indexes of oxidative stress improved more significantly in the observation group (P<0.05). Compared with before treatment, traditional Chinese medicine (TCM) syndrome score of two groups were significantly improved after treatment, with statistically significant differences (P<0.05). TCM syndrome score of observation group was lower than that of control group, with statistically significant differences (P<0.05). Adverse reactions of the two groups had no statistically significant difference. Conclusion: Xingnaojing injection can effectively alleviate the oxidative stress response and inflammatory response, reduce the neurological deficit and improve the state of consciousness, with an obvious curative effect in the treatment of acute brain.关键词：Xingnaojing injection;acute cerebral hemorrhage;inflammatory reaction;oxidative stress reaction;traditional Chinese medicine (TCM) syndrome score
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of modified Shenfu Yangrong Tang in treating viral myocarditis (VMC) and observe its effects on serum levels of interleukin (IL)-17, IL-27, nuclear transcription factor (NF)-κ B, IL-6, and IL-9. Method: One hundred and forty patients with VMC were randomly divided into control group and observation group with 70 cases in each group. The patients in control group were treated with western medicine comprehensive intervention measures. Based on the treatment in control group, in patients in observation group were also treated with modified Shenfu Yangrong Tang, 1dose/day and twice daily in morning and night. The treatment course was 8 weeks for both groups. Scores of syndrome of deficiency of both Yin and Yang, serum levels of serum central free fatty acid binding protein (H-FABP), Troponin I (cTnI), total efficacy and adverse reactions were compared between two groups. In addition, the levels of IL-17, IL-27, IL-6, IL-9 and NF-κ B in serum were detected in both groups. Result: After treatment, scores of syndrome of deficiency of both Yin and Yang (palpitate, breathe hard, limb cold and chills, dysphoria, panting, hidrosis, and edema), serum levels of H-FABP and cTnI in observation group were significantly lower than those of control group (P<0.01). Total efficacy was 91.18% in observation group, higher than 76.12% in control group (P<0.05). The incidence of adverse reactions such as headache in the observation group was lower than that in the control group, and nausea, vomiting and diarrhea were significantly less than those in the control group (P<0.05). Serum levels of IL-17, NF-κ B, IL-6, and IL-9 in observation group were significantly lower, while IL-27 was higher than those in control group after treatment (P<0.01). Conclusion: On this basis of conventional western medicine, modified Shenfu Yangrong Tang plays a good therapeutic effect and is safe in treating chronic stage of VMC,and its mechanism may be associated with regulating serum levels of IL-17, IL-27, NF-κ B, IL-6, and IL-9.关键词：Shenfu Yangrong Tang;acute viral myocarditis;chronic stage;syndrome of deficiency of both Yin and Yang
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of Yunpi Zhushi decoction in treating hypertension patients with spleen damp-heat type metabolic syndrome (MS), and observe its effect on serum levels of adiponectin (ADP), high-sensitive-C-reactive protein(hs-CRP), homocysteine(Hcy), endothelin (ET),and vascular von willebrand factor(vWF). Method: One hundred and eighteen patients with MS hypertension were randomly divided into control group and treatment group (59 cases in each group). The patients in control group orally took Telmisartan tablets (40 mg/time and 1 time/day). Based on the treatment in control group, the patients in treatment group were given with Yunpi Zhushi decoction, 1 dose/day, twice daily in night and morning. All patients were continuously treated for 3 months. Then indexes of blood pressure variability, physique study, blood fat, and blood glucose were compared between two groups. And the serum levels of ADP, hs-CRP, HCY, ET, and vWF were detected in both groups. Result: After treatment, 24 h diastolic pressure standard deviation (24 hDSD), diastolic pressure standard deviation at night (nDSD), systolic pressure standard deviation at daytime (dSSD),and diastolic pressure standard deviation at daytime (dDSD) were obviously decreased in both groups (P<0.01), but there was no statistically significant difference between two groups. After treatment, indexes of physique study, blood fat, and blood glucose were obviously decreased in treatment group, and lower than those of control group (P<0.01). Serum level of ADP in treatment group was remarkably higher, while hs-CRP, HCY, ET, and vWF were lower than those in control group with statistically significant differences (P<0.01). Conclusion: Based on western medicine, Yunpi Zhushi decoction has obvious efficacy in treating hypertension patients with spleen damp-heat type metabolic syndrome, and can up-regulate serum level of ADP and down-regulate serum levels of hs-CRP, Hcy, ET, and vWF.关键词：Yunpi Zhushi decoction;metabolic syndrome;hypertension;mechanism
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发布时间：2024-01-04 - 摘要：Generic medicines account for the vast majority of listed chemicals in China.Due to generic drugs are of variable quality,directly affects people's medication safety and effectiveness,at present,our country puts forward to improve the quality of generic drugs comprehensively,it is an important strategic opportunity for the pharmaceutical industry to evaluate the consistency of the quality of generic drugs and reference drugs in batches.Under the market-oriented,guide enterprises to participate in the construction of bioequivalence (BE) research platform through the way of horizontal cooperation and enterprises as the main body.The reference preparation and the information sharing mechanism for the consistency evaluation of generic drugs was established by the joint venture.At the same time,through the government to guide the allocation of resources,and take care of the central and western regions to solve the practical problems faced by pharmaceutical companies in central and western regions,in order to realize the regional balanced development of the pharmaceutical industry and promote the supply-side reform of the pharmaceutical industry.关键词：consistency evaluation of generic medicines;resource sharing;bioequivalence;platform construction
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发布时间：2024-01-04 - 摘要：The mechanism of Ephedrae Herba treating mass in abdomen was analyzed by combining the ancient and modern literatures in order to provide basis for expanding the application range of Ephedrae Herba.The items of Ephedrae Herba in previous and modern literatures were reviewed,and the special application of Ephedrae Herba was collected.Summarized the characters of Ephedrae Herba in Yanghetang and Mahuang Xixin Fuzitang in the treatment of gynecological mass in abdomen by combining the clinical practice.The mechanism of treating mass in abdomen was warm channel to expel cold,induce sweat to scatter evil,ventilate the lung-Qi,invigorate the kidney-Yang.Ephedrae Herba was mainly used in Yanghetang and Mahuang Xixin Fuzitang to treat gynecological mass in abdomen.There was great prospect of traditional Chinese medicine in treating mass by the flexible application of Ephedrae Herba,and it could expand the application range of Ephedrae Herba in gynecological diseases.关键词：Ephedrae Herba;treating mass in abdomen;gynecological mass in abdomen;Yanghetang;Mahuang Xixin Fuzitang
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发布时间：2024-01-04 - 摘要：Talc has been widely used in modern pharmaceutical preparations as a new pharmaceutical excipients in the 2015 edition of Chinese Pharmacopoeia.In this paper,the application of talc in preparation,including dispersant,disintegrating agent,lubricant,anti-sticking agent,coating layer and filter aid,to improve the critical relative humidity as well as to inhibit or promote the release of drugs were reviewed.At the same time,it summarizes the existing problems in the application process of talc.In the process of creating this paper,it is found that talc has excellent application prospect as a pharmaceutical excipient.This paper will provide theoretical support for later research of talc.关键词：talc;pharmaceutical excipients;dispersing agents;disintegrating agents;lubricants;anti-sticking agents
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发布时间：2024-01-04 - 摘要：Stroke is one of the major causes of global death and disability; and ischemic stroke is the most common type of stroke. In recent years, with the development of regenerative medicine, stem cell therapy has become a hotspot. Studies have confirmed that stem cell therapy, including activation of endogenous neural stem cells and exogenous stem cell transplantation, can promote endogenous nerve and vascular regeneration, repair nerve damage, so as to improve neurological function, with a broad prospect in the treatment of ischemic stroke. However, survival, migration and differentiation rates of stem cells remain low, with no effective solution. Traditional Chinese medicine(TCM) have been applied in the treatment of stroke for thousands of years. Recently, researchers have found that TCM can promote proliferation, differentiation and other physiological activities of stem cells in vitro and in vivo. TCM combined with stem cell therapy has been used to treat multiple diseases. Further studies have found that TCM can activate endogenous neural stem cells of ischemic brain tissues, and promote nerve repair, with a synergistic effect on transplanted stem cells. To provide theoretical support for subsequent studies, we summarized the studies of ischemic stroke in recent years and reviewed the progress of studies on the effect of TCM with stem cell therapy in treating ischemic stroke.关键词：Chinese herb medicine;brain ischemia;stem cell therapy;proliferation;differentiation;migration
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发布时间：2024-01-04 - 摘要：Vaginal disease, which is also known as vaginal inflammation, is a common and frequent disease among gynecological outpatients. Specifically, it refers to the inflammation of vaginal mucosa and submucosa-related tissues. Bacterial vaginosis (BV) and candidal vaginitis (CV) are two common vaginal diseases, which cause great impacts on women's daily life and study. At present, therapies for BV and CV have been improved from previously western medicine or antibiotic therapy into today's complete traditional Chinese medicine (TCM) prescriptions and integrated TCM and western medicines. And there has been a good development in the prevention of BV and CV. Compared with western medicine or antibiotics,TCM can be applied based on syndrome differentiation, with a stable and better curative effect and less side effects, and thus being easily accepted by patients. The restoration of a normal vaginal microbial environment is the foundation for curing vaginal disease, which is the purpose of TCM in the treatment of vaginal diseases. However, there are many kinds of Chinese medicinal herbs that can be used to treat BV and CV, and the treatment mechanisms are complex. In this paper, we consulted Chinese and English literatures in authoritative databases in recent years, and discusses pathogenesis of BV and CV, common TCM for the treatment of BV and CV, and their treatment mechanism, in order to provide new ideas for treating recurrent BV and CV and new drug R&D. The paper summarizes the three kinds of BV pathogenesis and five processes of CV pathogenesis. According to the statistics, there are 123 kinds of main TCM for treating BV, 133 kinds of main TCM for treating CV, and 84 kinds of main TCM for treating both diseases. And the main medicinal ingredients for treating BV and CV include alkaloid, glycosides, phenols, volatile oil, chlorogenic acid, flavonoids, pigments, and coumarin. TCM mechanisms for treating BV and CV are vitro antibacterial experiments, penetration increase of other antimicrobial agents, intracellular solution leakage, improvement of body immunity, and treatment with integrating traditional Chinese and western medicine. Finally, the paper shows some feasible suggestions on the problems existing in the treatment of BV and CV with TCM.关键词：traditional Chinese medicine;bacterial vaginosis;candidal vaginitis;pathogenesis;treatment mechanism;advance of studies
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发布时间：2024-01-04
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