最新刊期
卷 23 ， 期 20 ， 2017
- 摘要：Objective: To analyze color difference of Polygoni Multiflori Radix with different processing method and processing degree combined with traditional traits,and supply a research basis for color data standard of this herb. Method: Traits of processed products of Polygoni Multiflori Radix were observed,CM-5 colorimeter was adopted to measure L,a,b values of samples,E (total color value) and ΔE (color difference) were calculated.Cluster analysis and SAS 9.2 statistical software were employed to analyze the correlation between samples with different processing degree and the test result. Result: Color of the raw products from 2 origins and the control pieces powder of raw products was yellowish-white,the E was 70.31±3.The color of control pieces powder of Polygoni Multiflori Radix Praeparata was chocolate brown,the E was 52.24,compared with control pieces powder of raw products,the ΔE was 18.63.The color of Polygoni Multiflori Radix deepen after processing,and E decreased, variation laws of the 4 processing methods were the same.With the processing time extended,the color of samples gradually from claybank,light brown,dark brown,chocolate brown and until became brownish-black,the E gradually reduced from 61.11 to 30.45,E could indicate processing degree of Polygoni Multiflori Radix.It can distinguish the raw products and processed products by cluster analysis. Conclusion: Colorimeter tests can distinguish the raw products and processed products of Polygoni Multiflori Radix,and it can objectively and accurately express powder color change of Polygoni Multiflori Radix Praeparata with different processing degree.关键词：Polygoni Multiflori Radix;processing;traditional traits;color difference;Polygoni Multiflori Radix Praeparata
- 2
- |
- 3
- |
- 11
发布时间：2024-01-04 - 摘要：Objective: To optimize preparation technology of citronellol microemulsion gel and study on its in vitro antibacterial property. Method: The prescription of citronellol microemulsion was optimized by pseudo-ternary phase diagram with the percentage of microemulsion area as index.Single factor tests and orthogonal test were adopted to optimize formulation technology of citronellol microemulsion gel with appearance,viscosity,ductility,greasiness as indexes.Tube double dilution method was used to investigate minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of citronellol microemulsion gel to the tested bacteria.The bactericidal efficiency of citronellol microemulsion gel and citronellol microemulsion were tested by the experiment of time-sterilization efficiency. Result: Optimum prescription of citronellol microemulsion gel was as follows:citronellol of 0.518%,Kolliphor HS 15 of 1.037%,ethanol of 0.667%,carbomer-980 of 0.8%,glycerol of 5%,water of 91.978%.The acquired microemulsion exhibited nattier blue,it was uniform and transparent with the average particle size of 54.9 nm and Zeta potential of -10.22 mV.The MIC and MBC of citronellol microemulsion gel were 1.667 g ·L-1 for the tested bacteria(Staphylococcus aureus and Candida albicans).Time-sterilization efficiency indicated sterilizing rates were 100% of citronellol microemulsion gel to the tested bacteria at 2 h. Conclusion: This optimized formulation technology of citronellol microemulsion gel is stable and feasible,this preparation has a rapid bactericidal effect on S. aureus and C. albicans.关键词：citronellol;microemulsion;gel;formulation technology;in vitro antibacterial test;pseudo-ternary phase diagram method;tube double dilution method
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC fingerprint of Angong Niuhuangwan and evaluate its quality by similarity calculation and chemical pattern recognition. Method: HPLC was uesd,the separation was performed with the mobile phase consisting of acetonitrile and 0.1% phosphoric acid aqueous solution,and the detection wavelength was set at 230 nm.The fingerprints of 10 batches of Angong Niuhuangwan were analyzed by similarity analysis,and further evaluated the quality by principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA). Result: HPLC fingerprint of Angong Niuhuangwan was established,the similarity was above 0.9,and 25 peaks were recognized as common peaks,10 of them were identified by the reference substance.It was found that there were some differences among the samples from different years,and 14 differential components that causing differences in samples from different years were found by OPLS-DA. Conclusion: The established analytical method is simple,accurate and reliable.The combination of fingerprint with chemical pattern recognition can evaluate the quality of Angong Niuhuangwan well and it can provide scientific basis for quality control of this preparation.关键词：Angong Niuhuangwan;fingerprint;principal component analysis;orthogonal partial least squares discriminant analysis;chemical pattern recognition;berberine hydrochloride;similarity
- 6
- |
- 3
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: In this paper,taking Berberis amurensis root and Fomes fomentarius fruitbody as raw materials,based on the theory of compatibility of traditional Chinese medicine,the preparation method was used to develope Xiaobo Muti oral ulcer film. Method: Orthogonal test was adopted to optimize the formula of Xiaobo Muti oral ulcer film with comprehensive score of moldability,appearance,softness and toughness as index.Efficacy of the film was investigated by the establishment of rat oral ulcer animal model,female rats were randomly divided into blank group,model group,positive control group,high,medium and low dose group of Xiaobo Muti oral ulcer film.The positive control group was administered with propolis oral cavity membrane,one tablet each time,two times a day.The high dose group of Xiaobo Muti oral ulcer film was given 4 times within one day,4 tablets each time.The medium dose group of Xiaobo Muti oral ulcer film was administered 2 times a day,2 tablets each time.The low dose group of Xiaobo Muti oral ulcer film was administered once a day,one tablet each time.These all were given for a period of 7 days. Result: The best formula of Xiaobo Muti oral ulcer film was B. amurensis root extract-F. fomentarius fruitbody extract-starch-sodium carboxymethyl cellulose-glycerin-refining honey-water (8:4:10:2:3:20:500),under this formula,the score of each index was as follows:23.6 points of moldability,23.8 points of appearance,22.5 points of softness,23.3 points of toughness. Conclusion: Xiaobo Muti oral ulcer film has antibacterial and anti-inflammatory,it can promote healing effect for treatment of oral ulcer significantly.B. amurensis root and F. fomentarius fruitbody can work together to compose prescription,which can enhance the effect of treatment of oral ulcer caused by the fire in the spleen,stomach and liver.关键词：Berberis amurensis root;Fomes fomentarius fruitbody;oral ulcer;film;formula;mutual promotion;propolis oral cavity membrane;comprehensive score
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Astragalus polysaccharide is an important active ingredient with the immune regulation,anti-oxidation,anti-cancer,anti-atherosclerosis and so on.To remove the proteins from astragalus polysaccharide is a critical procedure,because the content of protein in astragalus polysaccharide was higher than 15%.In this work, the purity would be improved by removing the proteins from astragalus polysaccharide,which can be in favor of the study on pharmacological activity and mechanisms of this effective site. Method: Astragalus polysaccharide was prepared by the water-extraction and alcohol-precipitation method,and the proteins in astragalus polysaccharide were removed by enzymatic-Sevage process,then the technolology was optimized with orthogonal test and single factor tests,respectively.Optimum technology was selected based on the purity of astragalus polysaccharide and the content of proteins. Result: Optimum process of deproteinization was as follows:the enzyme-substrate ratio of 2.0%,pH of 5.0,hydrolysis for 24 h at 50℃;under these conditions,the removal rate of proteins and the content of astragalus polysaccharide were 87.80% and 83.47%,respectively. Conclusion: This optimal technology is stable and feasible,enzymatic-Sevage method can significantly improve the purity of astragalus polysaccharide.关键词：Astragali Radix;polysaccharides;removal of protein;enzymatic-Sevage method;process optimization
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To establish a method for structural identification of the chemical constituents of alkaloids from Dendrobium nobile stem by using UPLC-ESI-Orbitrap-MS. Method: Thermo Q Exactive quadrupole rod-electrostatic field orbitrap high resolution mass spectrometry-MS system was used and the chromatography separation was performed on Hypersil Gold C18 column (2.1 mm×150 mm, 1.9 μm) with 0.1% formic acid solution (A)-acetonitrile (B) as the mobile phase for gradient elution (0-2 min, 5%B; 2-15 min, 5%-95%B; 15-17 min, 95%B).The flow rate was set at 0.3 mL · min-1; the column temperature was 40℃, and the sample size was 2 μL.Electrospray ionization (ESI) source was applied and operated in full scan/targeted-ddMS2 under positive ion mode. Result: A total of 8 sesquiterpene alkaloids were identified based on the reference compounds, high resolution mass number and comparison between experimental data and published data.They were dendrobine, mubironine B,dendramine, dendroxine, nobilonine, 6-hydroxynobiline, N-isopentenyldendrobinium, and N-isopentenyl-6-hydroxydendroxinium. Conclusion: This study introduces a comprehensive analysis method for main chemical ingredients of alkaloids from D.nobilestem, and lays a foundation for the further analysis on the substance basis research, providing a significant guidance for identification and quality control of herbal D.nobile stem.关键词：Dendrobium nobile stem;sesquiterpene alkaloids;UPLC-ESI-Orbitrap-MS
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Scutellaria galericulata. Method: The herb of S.galericulata was crushed and then extracted with 95% ethanol.The extracts were combined and concentrated under reduced pressure.The extracts were dispersed in water and respectively extracted with petroleum ether, ethyl acetate, chloroform and n-butanol to get petroleum ether fraction, ethyl acetate fraction, chloroform fraction and n-butanolfraction.Ethyl acetate fraction were separated and purified by silica gel column chromatography, Sephadex LH-20 and HPTLC.The structures of the glycosides compounds were identified by their physico-chemical properties and spectral data. Result: Eleven compounds were isolated and purified as:isocrenatoside (1),osmanthuside B6 (2), eutigoide A (3), plantainoside C (4), calceorioside B (5), isomartynoside (6), verbascoside (7), acteoside (8), desrhamnosyl acteoside (9), 5,7-dihydroxy-8,2'-dimethoxyflavone-7-O-β-D-glucopyranoside (10), and apigenin-7-O-rhamnoside (11). Conclusion: Compounds 1-4 and 7-11 were isolated from S.galericulata for the first time, and the literature review showed that plantainoside C, apigenin-7-O-rhamnoside and other compounds had anti-inflammatory and antioxidant activities, providing a good reference for further studies onpharmacodynamic material basis and the development and utilization of S.galericulata.关键词：Scutellaria;S. galericulata;phenylethanoid glycoside;flavonoid glycoside
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of triterpenoids in the heartwood of Dalbergia latifolia. Method: The heartwood samples of D.latifolia were crushed into powder and extracted with 70% ethanol for 3 times, 2 h for each time.The filtrate liquid was collected and the solvent was decompressed and recovered to obtain extract.The extract was dispersed in water and then extracted successively with petroleum ester, dichloromethane, ethylacetate and butanol to obtain petroleum ester fraction, dichloromethane fraction, ethylacetate fraction and butanol fraction.The chemical compounds in the extract fractions were isolated by column chromatography technologies such as silica gel, Sephadex LH-20, reverse phase silica gel and macroporous resin, and the structures of the obtained monomeric compounds were identified through physical and chemical analysis, spectroscopic methods and mass spectrum analysis method. Result: Seven triterpenoids were isolated and identified as 3-O-α-L-arabinopyranosyl-28-O-β-D-glucopyranosyl pomolic acid (1), β-sitostenone (2), β-sitosterol (3), stigmasterol (4), β-amyrin acetate (5), β-amyrin-3-palmitate (6), and 3-acetoxy-oleanoic acid (7). Conclusion: Compounds 1, 5, and 6 were isolated from Dalbergia genus for the first time.Compounds 2 and 4 were isolated from this plant for the first time.关键词：Dalbergia latifolia;triterpenoids;structure identification
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from roots of Salvia castanea. Method: The ethyl acetate fraction of 70% ethanol extract of S.castanea was isolated and purified by silica gel column chromatographyand HPLC.Then their structures were elucidated by physicochemical properties and spectral data. Result: Twelve compounds were isolated and elucidated as n-hexacosane (1),β-sitosterol (2),3-O-acetyl oleanolic aldehyde (3), oleanolic acid(4), 5,5'-dibutoxy-2,2'-bifuran(5), butachlor(6), ethyl linolenate(7), caffeoyl ethyl ester (8), vanillic acid (9), protocatechuicacid (10), protocatechualdehyde(11), and 5-hydroxymethyl-2-furancarboxaldehyde(12). Conclusion: Compounds 3, 5, and 6 were isolated from genus of Salvia for the first time; compounds 1, 4, 7-9 and 12 were first obtained from this plant.关键词：Salvia castanea;chemical constituents;isolation and identification
- 2
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To explore the relationship between long-term stability and accelerated stability of certified reference materials (CRMs) of natural products,in order to provide technical support for the prediction of validity of CRMs. Method: In the process of development of 5 CRMs(cinnamaldehyde,sakuranetin,pedunculoside,stachyose and β-sitosterol),the long-term stability test was carried out by determining their purities at 0,1,2,3,6,9,12,18,24 months under the temperature of 0-8℃ and the accelerated stability test was carried out by determining their purities at 0,1,2,3,6 months under temperature of (40±2)℃ and relative humidity of (75±5)%. Result: The results of statistical analysis showed that both long-term stability and accelerated stability were excellent and there were no significant difference between long-term stability and accelerated stability. Conclusion: The validity of 24 months of CRMs can be predicted on the bases of the result of accelerated stability test in 6 months.关键词：certified reference materials;accelerated stability;long-term stability;validity
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To simultaneously determinate the contents of protocatechuic acid, chlorogenic acid, hyperoside, specnuezhenide, epmedin C and icariin in Bushen Qiangshen pills (BQP) by developing an Ultra high performance liquid method(UPLC). Method: The UPLC separation was performed on an Agilent SB-C18 reversed-phase column (4.6 mm×100 mm,2.7 μm) with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution.The detective wavelength was set at 260, 325, 360, 224, 270 nm. Result: The calibration curve was linear when amount was within 0.78-201.00 mg ·L-1 for protocatechuic acid (r=0.999 9); 0.79-202.60 mg · L-1 for chlorogenic acid (r=0.999 7); 0.93-238.39 mg ·L-1 for hyperoside (r=0.999 9); 0.66-169.62 mg ·L-1 for specnuezhenide (r=0.999 9); 0.81-206.85 mg ·L-1 for epmedin C (r=0.999 9); and 0.74-190.85 mg ·L-1for icariin (r=0.999 9).The recoveries were 97.6%, 99.6%, 99.7%, 97.2%, 95.5%, 96.3% with RSDs of 1.0%, 1.5%, 1.5%, 0.8%, 0.4% and 0.9%, respectively. Conclusion: This simple and reliable method can be used for the quality control of BQP.关键词：Bushen Qiangshen pills;protocatechuic acid;chlorogenic acid;hyperoside;specnuezhenide;epmedin C;icariin
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneously determining the contents of puerarin, thymol, carvacrol, honokiol, hesperidin and daidzin in Jiawei Xiangru oral liquid (Puerariae Lobatae Radix, Moslae Herba, Magnoliae Officinalis Cortex, Citri Reticulatae Pericarpium, Bombyx Mori Linnacus and Lablab Semen Alabum). Method: High performance liquid chromatography (HPLC) was performed on Hypersil C18 chromatographic column (4.6 mm×250 mm, 5 μm) with mobile phase of acetonitrile-0.01% glacial acetic acid (gradient elution) at a flow rate of 1 mL · min-1.The detction wavelength was 277 nm, and the column temperature was 30℃. Result: Puerarin, thymol, carvacrol, honokiol, hesperidin and daidzin showed good linear relationship between injection volume and peak area within the range of 0.384-4.800 μg (r=0.999 3), 0.048-0.600 μg (r=0.999 7), 0.015 6-0.195 0 μg (r=0.999 8), 0.018 2-0.227 5 μg (r=0.999 2), 0.226-2.825 μg (r=0.999 6), and 0.228-2.850 μg (r=0.999 5),respectively.The average recovery rate was 97.98%, 98.88%, 98.10%, 100.12%, 100.40% and 96.70%, respectively for puerarin, thymol, carvacrol, honokiol, hesperidin and daidzin, with RSDs of 2.1%, 1.8%, 2.1%, 1.5%, 2.6%, and 1.6%. Conclusion: The method is simple, stable and reproducible, and it can be used for the simultaneous determination of puerarin, thymol, carvacrol, honokiol, hesperidin and daidzin contents in Jiawei Xiangru oral liquid.关键词：puerarin;thymol;carvacrol;honokiol;hesperidin;daidzin;HPLC;Jiawei Xiangru oral liquid
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC chromatographic fingerprint analysis method for the quality evaluation of Calonyctii Semen. Method: The analysis was carried out by Agilent Extend-C18 column (4.6 mm×250 mm, 5 μm), with acetonitrile (A)-0.1% formic acid solution (B) as the mobile phase for gradient elution.The temperature of column was 30℃ at a flow rate of 1.0 mL · min-1; the detection wavelength was set at 300 nm, and the injection volume was 10 μL.The HPLC fingerprint was analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2012).The comparison with reference drugs, cluster analysis, principal component analysis and partial least square discriminant analysis (PLS-DA) were used for analysis. Result: The precision, repeatability and stability of the established method can meet the requirements of fingerprint chromatography analysis.There were 14 common peaks in the HPLC fingerprint.The similarity of 10 batches of samples from Guangxi was greater than 0.70, indicating great differences in quality of Calonyctii Semen from different areas of Guangxi.Through the cluster analysis, principal component analysis and partial least square discriminant analysis (PLS-DA), the samples were divided into three categories and showed that the producing origins and collection time were correlated with the quality of medicinal herbs. Conclusion: The developed HPLC fingerprint method is quick, simple, and could comprehensively reflect the chemical components information of alkaloids for Calonyctii Semen.Therefore, it could be used in quality evaluation of Calonyctii Semen.The samples collected in Yulin city and Guilin city have better quality, so these two cities can be the producing origins for Calonyctii Semen.关键词：Calonyctii Semen;HPLC;fingerprint;similarity;Guangxi
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish a new technology for Fritillariae Thunbergii Bulbus quality control based on effect-constituent index, with resolving phlegm as the main effects and associated with Fritillariae Thunbergii Bulbus clinical efficacy, and make the new quality control method better promote the development of Fritillariae Thunbergii Bulbus industry. Method: Fritillariae Thunbergii Bulbus samples with different varieties and different origins were collected and the upper and lower limits of active ingredients contents (peimine and peiminine) were determined.The analysis conditions were as follows:Hypersil ODS-3 column (4.6 mm×250 mm, 5 μm); with acetonitrile-water-diethylamine (70:30:0.03) as the mobile phase; flow rate at 0.8 mL · min-1; the drift tube temperature of Alltech2000-ELSD detector was 85℃,carrier gas flow rate at 2.2 L ·min-1.In the range of concentrations determined, different concentrations of peimine and peiminine standard solution were prepared.With the concentration of phenol red as the phlegm effect indicator in the mouse tracheal phenol red expectoration experiment, the linear relationship between the mouse phenol red excretion and the mass concentration of peimine and peiminine was analyzed to fit the dose-response equation f(Xi) between peimine, peiminine and resolving phlegm effect indicators.Thus the resolving phlegm effect index was constructed and verified. Result: In the main producing areas of Fritillariae Thunbergii Bulbus, the content of peimine was between 0.046 5%-0.092 5%, and the content of peiminine was between 0.017 5%-0.054 3%.Therefore, the range of dosing solution concentration was:peimine content in the range of 0.04%-0.10% and peiminine content in the range of 0.02%-0.06%.The results of mouse tracheal phenol red expectoration experiment showed that peimine in 0.09-0.23 g · L-1 and peiminine in 0.045-0.135 g·L-1 range had a good linear relationship with the concentration of mouse tracheal phenol red (rpeimine=0.994 7, rpeiminine=0.997 8).The established resolving phlegm effect-constituent index of Fritillariae Thunbergii Bulbus according to the fitted dose-response equation can characterize its expectorant efficacy through the contents of the compositions. Conclusion: The establishment of resolving phlegm effect-constituent index, has not only achieved dose-efficacy correlation, but also achieved the concept of observing effect by the composition contents.Therefore, it has better guiding significance and application prospects for Fritillariae Thunbergii Bulbus quality control assessment, industrial development and clinical rational drug use.关键词：Fritillariae Thunbergii Bulbus;resolving phlegm;effect-constituent index;content determination.
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To study and compare the fat-soluble components from the roots, stems, leaves and fruits of Sedum aizoon. Method: The fat-soluble components from different parts of S.aizoon were extracted by soxlet extraction.The fat-soluble components were esterified by using potassium hydroxide-methanol direct esterification method and then analyzed by gas chromatography-mass spectrometry (GC-MS).Carrier gas was high-purity helium; sample injection temperature was 250℃; velocity of flow was 1.24 mL · min-1; ionization mode was EI ion source, with ion source temperature of 200℃, interface temperature of 250℃, solvent delay of 3.5 min, m/z 40-600. Result: The 26, 37, 37 and 20 kinds of fat-soluble components were identified from roots, stems, leaves and fruits respectively.12 kinds of them were contained in all of the four parts, while 6, 6, 9 and 2 kinds were only present in roots, stems, leaves or fruits respectively.The main components of fat-soluble components were unsaturated fatty acids, which were mainly composed of linolenic acid and linoleic acid.The contents of unsaturated fatty acids were 42.59%, 52.57%, 37.04% and 80.06% respectively in roots, stems, leaves and fruits.The ratios of unsaturated fatty acids to saturated fatty acids in the different parts were 1.17:1, 1.38:1, 0.85:1 and 4.56:1.The four parts had similar fatty acid compositions, but there were some differences in the composition ratios. Conclusion: The fat-soluble components of S.aizoon were extracted, identified and compared for the first time.This research could establish foundation for further research and development of S.aizoon.关键词：Sedum aizoon;fat-soluble components;GC-MS;roots;stems;leaves;fruits
- 2
- |
- 4
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Scrophulariae Radix on rat model with dampness stagnancy due to spleen deficiency,and to analyze biomarkers and metabolic pathways based on liver metabonomics. Method: The high fat and low protein diet plus swimming for 8 weeks under weight bearing condition was adopted to establish rat model with dampness stagnancy due to spleen deficiency.Liver index,serum albumin,blood fat,gastrointestinal function and water load were measured at 2 weeks after administration of high(5.40 g · kg-1) and low(1.35 g · kg-1) dose of Scrophulariae Radix decoction.UPLC-Q-TOF-MS was employed to analyze metabolites in liver tissue under electrospray ionization positive and negative ion mode with m/z 100-1 500.Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were adopted to compare the differential metabolites among the normal group,the model group,the high and low dose group of Scrophulariae Radix decoction;these different metabolites among four groups were analyzed by KEGG metabolic pathway method. Result: Compared with the model group,gastric emptying rate and D-xylose excretory rate in the high dose group were increased significantly;contents of serum albumin and total protein in the low dose group were increased;contents of low density lipoprotein-cholesterol,total cholesterol and water load index in the high dose group were decreased.A total of 21 biomarkers were identified and 7 major metabolic pathways were found,which mainly related to the insulin secretion,purine metabolism,bile acid secretion and so on. Conclusion: Treatment of Scrophulariae Radix on rat model with dampness stagnancy due to spleen deficiency is connected with improving the digestive function,inhibition of glucose metabolism,improving blood lipid metabolism and so on.关键词：Scrophulariae Radix;dampness stagnancy due to spleen deficiency;metabonomics;biomarkers;metabolic pathway;xanthine;phosphatidyl ethanolamine
- 2
- |
- 3
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: It was explored in the article that the compatibility connotation of Aconiti Radix and Pinelliae Rhizoma (PR) after the different processed products of PR was added to Wutoutang (WTT) from cytochrome P450 enzyme system (CYP) 3A1/2 medating metabolic herb-herbs interaction in rats. Method: The change of CYP3A enzymatic activity was detected by incubation in vitro and probe method in vivo.The expression of protein and mRNA was determined with Western blot and the fluorescence quantitative polymerase chain reaction (PCR) technology. Result: By comparing with WTT adding unprocessed Pinelliae Rhizoma (UPR),the production rate of 6β-hydroxy-testosterone (6β-OH-Tes) did not change and the expression of CYP3A protein were increased (P<0.01,P<0.05) in WTT adding Pinelliae Rhizoma Praeparatum (PRP),the mRNA expression of CYP3A1 was decreased (P<0.01) only in 0.60 g · kg-1 group of WTT adding PRP;the production rate of 6β-OH-Tes was very dramatically decreased (P<0.01) in 1.20 g · kg-1 group of WTT adding Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine (PRPZA),the expression of CYP3A protein was increased (P<0.05) only in 0.60 g · kg-1 group of WTT adding PRPZA,the mRNA expression of CYP3A1 were markedly decreased (P<0.01) in 0.60,1.20 g · kg-1 groups of WTT adding PRPZA;the ratios between AUC0-t of 6'-hydroxy-buspirone (6'-OH-BP) and AUC0-t of buspirone (BP),AUC0-t of 1-(2-pyrimidinyl)-piperazine (1-PP) and AUC0-t of BP did not change in WTT adding PRP/PRPZA by comparing with WTT adding UPR. Conclusion: Different processed products of PR can enhance the inhibition of WTT on CYP3A activity in various degree,which may slower the metabolism of diester diterpenoid alkaloids(such as aconitine) in WTT,and then lead to increase the toxicity or the pharmacological action of WTT.关键词：Wutoutang;Pinelliae Rhizoma;cytochrome P450 enzyme system;testosterone;buspirone;polymerase chain reaction;pharmacokinetics
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To discuss the anti-inflammatory and joint protection effect of Fengshining (FSN) capsule in treatment of rheumatoid arthritis (RA) and its action mechanism. Method: The 120 male New Zealand white rabbits were randomly divided into normal control group, model group, Methotrexate group, low-dose, middle-dose and high-dose FSN groups, with 20 in each group.The collagen-induced arthritis (CIA) and wind-cold dampness rheumatoid arthritis model were established in the groups, except for the normal control group.Low-dose, middle-dose and high-dose FSN groups were given aqueous solution of 0.33, 0.66, 1.32 g · kg-1 FSN capsule respectively, the methotrexate group was given aqueous solution of 1.0 mg · kg-1 methotrexate tablets, while the normal control group and the model group were given saline solution.Each group was intragastrically administrated with 4 mL · kg-1of corresponding medicines, once a day for 7 days.The effect of FSN capsule on general conditions, joint swelling degree of the animal model were observed, and the expressions of inflammatory factor interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α messenger RNA(mRNA) and receptor activator of nuclear factor kappa B ligand (RANKL) mRNA in RA synovium tissues were detected with Real-time PCR method. Result: FSN capsule could improve the living state of RA rabbits significantly, reduce the swelling and arthritis score of stifle, and obviously decrease the expressions of IL-1β, IL-6, TNF-α and RANKL mRNA in RA synovial tissues. Conclusion: FSN capsule has the effect in inhibiting the expression of inflammation factor and RANKL in synovial tissues, and could effectively alleviate inflammation and bone damage, so as to show a curative effect on RA.关键词：Fengshining capsule;rheumatoid arthritis;wind-cold-dampness syndrome;synovial tissue;inflammatory factor;receptor activator of nuclear factor kappa B (NF-κB) ligand
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Danshen injection on expression of oligodendrocyte lineage transcription factor-2 (Olig-2) and OX-42 (markers of proliferation of oligodendrocyte and microglia) in rats after spinal cord injury (SCI), in order to explore its mechanism of promoting spinal cord functional recovery. Method: Fifty SPF SD rats were randomly divided into five groups (n=10), namely normal control group, model group, Danshen injection treatment group, Danshen injection+rapamycin group and methylprednisolone sodium group.All of the rats, except for normal control group, were included in the weight-drop SCI model by the Allens' method.Then, normal saline (1 mL · kg-1), Danshen injection (1 mL · kg-1) and Danshen injection (1 mL · kg-1, containing rapamycin 3 mg · kg-1) were intraperitoneally injected into model group, Danshen injection treatment group and Danshen injection+rapamycin group rats once a day, respectively, and methylprednisolone sodium solution (30 g · L-1) was injected into the methylprednisolone sodium group rats through tail vein at the dosage of 1 mL · kg-1 once a day.The rats in normal control group were fed with routine foods, with no injection.The combine behavioral score (CBS) of rats in all group were detected on 1, 3, 7, 14 d after SCI.All of these rats were put to death on 14 d after SCI, and the expressions of Olig-2 and OX-42 were detected with immunofluorescence staining and Western blot technique. Result: Compared with the model group and the Danshen injection+rapamycin group, CBS significantly declined in treatment group on 14 d after SCI (P<0.05).Compared with the normal control group, the expressions (including relative content and immunes histochemistry positive cells number) of Olig-2 declined, and OX-42 elevated in model group (P<0.05).Compared with the model group, the expressions of Olig-2 elevated and OX-42 declined in Danshen injection treatment group and methylprednisolone sodium group(P<0.05). Compared with the Danshen injection treatment group, the expressions of Olig-2 declined and OX-42 elevated in Danshen injection+rapamycin group (P<0.05).There was no statistically significant difference in these indicators between Danshen injection treatment group and methylprednisolone sodium group. Conclusion: Danshen injection could promote the recovery of nerve function after SCI by elevating the proliferation of OLS and inhibiting the activation of microglia, and the mechanism may be related to the activity of the phosphatidyl inositol 3-kinase(PI3K)/Akt/mTOR signaling pathway.关键词：spinal cord injury;Danshen injection;oligodendrocyte lineage transcription factor-2;OX-42;phosphatidyl inositol 3-kinase(PI3K)/Akt/mTOR
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of catalpol on damaged mouse brain microvascular endothelial cells (bEnd.3) induced by Aβ1-42, and explore the protective mechanism. Method: The bEnd.3 cells were divided into five groups:control group, model group (Aβ1-42 20 μmol · L-1), low, middle and high-dose catalpol groups (Aβ1-42 20 μmol · L-1+catalpol 50, 200, 500 μmol · L-1).Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was used to detect the effect of catalpol on bEnd.3 cell activity induced by Aβ1-42; Hoechst33258 staining was used to evaluate the effect of catalpol on apoptosis in bEnd.3 cells induced by Aβ1-42; Western blot method was to detect relevant protein expressions of autophagy and apoptosis, Beclin-1, LC3, Bax, Bcl-2, cytochrome-C, Caspase-3, cleaved Caspase-3. Result: The result of MTT showed, catalpol had no toxic and side effect on bEnd.3 cells; MTT and Hoechst staining results showed that low, middle and high-dose catalpol groups could significantly improve the activity of bEnd.3 cells and inhibit the apoptosis induced by Aβ1-42 (P<0.05), compared with model group; the results of Western blot showed that the ratio of Beclin-1/β-actin, LC3-Ⅱ/LC3-Ⅰ, Bax/Bcl-2, cytochrome-C/β-actin and cleaved Caspase-3/Caspase-3 obviously reduced after pretreatment with catalpol (P<0.05), compared with model group. Conclusion: Catalpol may have the neuroprotective effect by inhibiting bEnd.3 cell apoptosis and autophagy induced by Aβ1-42.关键词：Alzheimer's diseases;Aβ1-42;bEnd.3 cell;catalpol;apoptosis;autophagy
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To explore the regulating effect of Yishen Jiedu prescription on the protein expression of ribonuclease L (RNase L) and the apoptosis terminal protein Caspase-3 in bone metastasis from lung adenocarcinoma. Method: Lung adenocarcinoma cell lines that had won the national patent were injected into left ventricle of nude mice to prepare the bone metastasis model.Then, the mice were randomly divided into five groups:high, middle and low-dose (24,12,3 g · kg-1) Yishen Jiedu groups, disodium phosphate group and control group.Finally, with histopathological method and microSPECT/CT imaging method, we can observe the outcome of bone metastases.After administration at fixed time and dose for 5 weeks, dissection was performed, and the expressions of RNase L protein and Caspase-3 protein in bone metastasis from lung adenocarcinoma, and the regulatory effect of Yishen Jiedu prescription was analyzed through immunohistochemistry methods, Western blot and reverse transcription polymerase chain reaction (RT-PCR) methods. Result: The model group had the highest expression of RNase L protein and mRNA in bone metastases tumor cells, with significant statistical differences between the model group and others (P<0.01).However, the model group showed the lowest expression of Caspase-3 protein and mRNA, with significant statistical differences between the middle low dosage group of Yishen Jiedu and disodium phosphate groups (P<0.01). Conclusion: There was a high expression in RNase L expression in bone metastases in patients with lung adenocarcinoma, while Caspase-3, a main terminal protein in the process of cell apoptosis, was extremely low.The internal mechanism may be correlated with the cell apoptosis escape of lung adenocarcinoma bone metastases.The prescription of Yishen Jiedu prescription can inhibit the apoptosis of tumor escape by reducing the expression of RNase L and enhancing caspase-3 protein.关键词：Yishen Jiedu prescription;lung adenocarcinoma;bone metastasis;RNase L;Caspase-3
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effects of the containing serum of Xiaoai Jiedu decoction with different concentrations on proliferation and glycolysis of human colon cancer cells and investigate its action mechanism. Method: Eight new zealand rabbits were randomized into the serum containing medicine[40 g · (kg · d)-1 of Xiaoai Jiedu decoction]group and the blank serum group (equivoluminal normal saline).Both groups received intragastric administration for 3 days to prepare serum containing Xiaoai Jiedu decoction and blank serum.Human colon cancer HT-29 and HCT-116 cells were cultured with pharmacological serum of Xiaoai Jiedu decoction (5%, 10% and 15%), and then their effects on the human colon cancer cells' proliferation was examined by methyl thiazolyl tetrazolium assay.The amount of lactic acid produced by HT-29 and HCT-116 cells was measured by lactic acid test kit, and the amount of glucose produced by HT-29 and HCT-116 cells was measured by glucose test kit.The expression levels of hexokinase 2 (HK2), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase (LDHA), and Hypoxia inducible factor-1α (HIF-1α) were detected by using Western blot. Result: Serum containing Xiaoai Jiedu decoction exhibited suppressive effects on HT-29 and HCT-116 proliferation.The effect of 15% serum containing Xiaoai Jiedu decoction was the best, with inhibition rate of 42.7% and 50.2% respectively for HT-29 and HCT-116 cells.Western blot analysis indicated that as compared with blank control group, the serum containing Xiaoai Jiedu decoction reduced the expression of HK2, PDK1, LDHA and HIF-1α (P<0.05), and with the increase of concentration, the expression was decreased.The results of lactic acid and glucose test kit showed that as compared with the blank control group, the serum containing Xiaoai Jiedu decoction can reduce the levels of lactic acid and glucose (P<0.05) and with the increasing of concentration, the levels of lactic acid and glucose were decreased. Conclusion: The serum containing Xiaoai Jiedu decoction can inhibit the proliferation of human colon cancer HT-29 and HCT-116 cells by inhibiting the process of the glycolysis.Inhibitory mechanisms of glycolysis might be associated with reducing the expression of HIF-1α, thereby reducing the expression of glycolytic enzymes HK2, PDK1, and LDHA.关键词：Xiaoai Jiedu decoction;colon cancer;proliferation;glycolysis
- 2
- |
- 3
- |
- 9
发布时间：2024-01-04 - 摘要：Objective: To explore the mechanism of Fuzi Lizhongtang in warming Yang, invigorating the spleen and dispelling dampness through AQP4-ANP-pGC pathway. Method: A total of 120 clean grade Wistar rats were randomly selected and divided into blank control group, sham operation group, model group, and high, middle, low-dose Fuzi Lizhongtang groups, with 20 rats in each group.The blank control group was fed normally.Animal models were established in the sham operation group and the model group through interscapular brown adipose tissue (BAT) resection+high-fat diet+cold environment stimulation at alternate days (the sham operation group only received BAT without resection).The high-fat diet was fed for 30 days since the first day of the operation.In addition to the treatment for the model group, the three Fuzi Lizhongtang groups were respectively treated with high-dose (40 g · kg-1 · d-1), middle-dose (20 g · kg-1 · d-1) and low-dose (10 g · kg-1 · d-1) Fuzi Lizhongtang, while the other groups were given the same amount of normal saline.Materials were collected after 30 days of administration, (the model group was collected on the first day after successful modeling).The morphologic changes in gastric antrum and ileum in rats were observed by HE staining; ANP content was detected by ELISA; the mRNA expression of AQP4 was detected by Real-time PCR.And the AQP4 content was detected by Western blot. Result: HE staining of gastric antrum and ileum in rats was observed under light microscope.Pathological changes in gastrointestinal tissues in rats in each Fuzi Lizhongtang group were alleviated to different degrees, with the best effect in high-dose group.Compared with the control group and the sham operation group, the contents of AQP4, ANP and the relative expression of AQP4 mRNA in the model group decreased (P<0.05, P<0.01).Compared with the model group, the contents of AQP4, ANP and the relative expression of AQP4 mRNA in the middle and the high dose groups increased (P<0.05, P<0.01). Conclusion: Fuzi Lizhongtang may regulate body fluid metabolism in rats with spleen Yang deficiency syndrome by changing the contents of AQP4, ANP and the expression of AQP4 mRNA in AQP4-ANP-pGC pathway.关键词：Fuzi Lizhongtang;aquaporins(AQP);atrial natriuretic peptide(ANP);particulate guanylate cyclase(pGC)
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To compare the effect of herb pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma with different medicinal guides on hepatic fibrosis rats. Method: Sixty SD rats were randomly divided into normal group, model group, herb pair group (5.0 g · kg-1), herb pair plus Platycodonis Radix (5.0 g · kg-1+1.5 g · kg-1), herb pair plus Bupleuri Radix group (5.0 g · kg-1+1.5 g · kg-1) and herb pair plus Asari Radix et Rhizom group (5.0 g · kg-1+0.3 g · kg-1). The hepatic fibrosis rat models were induced through intraperitoneal injection with CCl4.Since the 3rd week after modeling, different drugs were administrated by gavage for 6 weeks. Rats were put to death after the last administration, and their serum was collected to measure activities of serum alanin aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Levels of laminin (LN), hyaluronic acid (HA) and procollagen Ⅲ (PCⅢ) in serum were detected by enzyme linked immunosorbent assay (ELISA). The content of hydroxyproline (HYP) in liver homogenate was measured by acid hydrolysis method. The expression of transforming growth factor-β1 (TGF-β1) in liver was analyzed by immunohistochemical method. Result: Compared with the model group, the levels of ALT, AST, ALP, LN, HA, PCⅢ and the content of HYP in experimental groups were significantly decreased, the expression of TGF-β1 in liver was reduced (P<0.05, P<0.01), the liver pathological fibrosis was obviously relieved. Compared with the herb pair group, the levels of ALT, AST, ALP, LN, HA, PCⅢ and the expression of TGF-β1 in the herb pair plus Bupleuri Radix group were significantly decreased (P<0.05, P<0.01). Conclusion: The herb pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma has a protective effect on liver fibrosis rat, and Bupleuri Radix could further improve the curative effect.关键词：Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma;hepatic fibrosis;medicinal guide;Bupleuri Radix;transforming growth factor-β1(TGF-β1)
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To observe the anti-osteoporosis effect of Ranae Oviductus protein (ROP), and the regulatory effect on bone growth related genes. Method: The osteoporosis model of rats were established by castration method, and randomly divided into 6 groups, namely blank group, model group, Estradiol valerate group, and high-does, medium-does and low-does (0.15, 0.075, 0.037 5 g ·kg-1) ROP groups.The bone mineral densities of femur and lumbar were measured by X-ray technique. The maximum load of femur was measured by bone strength instrument.Quantitative analysis was made for the expressions of key genes of bone growth by Real-time PCR. Result: Compared with the blank group, the BMD of femur and lumbar in the model group decreased significantly (P<0.05, P<0.01), the expressions of alkaline phosphatase (ALP), Osteocalcin and Runx-2 gene decreased significantly (P<0.01).Compared with the model group, the BMD of lumbar vertebrae in high and middle-dose groups increased significantly (P<0.01), the expressions of ALP, Osteocalcin and Runx-2 gene in the high-dose group were significantly increased (P<0.01). Conclusion: ROP can significantly improve bone mineral density and maximum load in rats, improve the mRNA expressions of ALP, Osteocalcin and Runx-2 gene, promote the osteoblast differentiation, and regulate the bone substitute balance, so as to play a role in preventing and treating osteoporosis.关键词：Ranae Oviductus protein (ROP);osteoporosis;alkaline phosphatase (ALP);Osteocalcin;Runx-2
- 2
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of anisodine hydromide on the expression of autophagy-related protein, in order to explore the protective effect and possible mechanism of anisodine hydromide on rats pheochromocytoma cells (PC12) induced by hypoxia. Method: PC12 cells were divided into control group, model group, n-butylphthalide group, and high-dose, middle-dose and low-dose anisodine hydromide groups (100, 50, 25 μmol · L-1).The relative viability of PC12 cells induced by hypoxia was measured by thiazole blue chromatomentry (MTT), and the morphology of PC12 cells was observed.The morphological changes in autophagosome were observed by transmission electron microscope and monodansylcadaverine (MDC) fluorescence staining.The expressions of microtubule associated protein 1 light chain3 (LC3) and Beclin-1 protein were detected by Western blot method. Result: The viability rates of cells were significantly improved after intervention with anisodine hydromide, and the expressions of LC3-Ⅱ and Beclin-1 were significantly improved after intervention with anisodine hydromide.These results were consistent with the observation of transmission electronmicroscope and MDC fluorescence staining. Conclusion: Autophagy has a protective effect on PC12 cell induced by hypoxia, and anisodine hydromide could prevent the injury of PC12 cells induced by hypoxia through activating autophagy.关键词：anisodine hydromide;rats pheochromocytoma cells (PC12);hypoxia;autophagy;neuroprotection
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To explore the effect of modified Buyang Huanwutang on the expression of Toll like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and tumor necrosis factor receptor-associated factor-6(TRAF-6), nuclear factor kappa B (NF-κB) in the apolipoprotein E gene knockout mice (ApoE-/- mice), in order to discuss the mechanism of modified Buyang Huanwutang for the prevention and treatment of atherosclerosis (AS). Method: Thirty male ApoE-/- mice were randomly divided into three groups:model group, modified Buyang Huanwutang group and atorvastatin group, and 10 C57BL/6J mice were the control group.Model group, modified Buyang Huanwutang group and atorvastatin group were given high-fat diet.At the same time, modified Buyang Huanwutang group and simvastatin group were intragastrically administrated with modified Buyang Huanwutang (18.69 g · kg-1), leech powder (0.39 g · kg-1) and atorvastatin (1.3 mg · kg-1).After 9 weeks of treatment, cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were detected.In addition, aorta segment was taken from the experiment animals to observe morphological changes by HE, and measure the ratio of the plaque area to the intravascular space.Real-time PCR and Western blot were used to determine the expressions of aortic TLR4, MyD88, TRAF-6, NF-κB. Result: In terms of blood lipid levels, compared with the normal group, TC, TG and LDL-C in the model group were significantly higher, while HDL-C levels was significantly lower (P<0.01).Compared with the model group, TC, TG and LDL-C in the modified Buyang Huanwutang group and the atorvastatin group were significantly lower, while HDL-C were significantly higher (P<0.01).HE staining showed no aortic plaque formation in C57BL/6J mice control group, and significant aortic plaque formation and increased inflammatory cell in model group.Compared with the model group, aortic vascular cells were arranged more orderly, inflammatory cell infiltration was reduced, and the proportion of aortic plaque in blood vessels reduced to varying degrees in Buyang Huanwutang group and atorvastatin group.To compared with C57BL/6J mice in the control group, mRNA and protein expressions of aortic TLR4, MyD88, TRAF-6 and NF-κB obviously increased in model group (P<0.01).mRNA and protein expressions of aortic TLR4, MyD88, TRAF-6 and NF-κB significantly decreased in both modified Buyang Huanwutang group and atorvastatin group, compared with model group (P<0.05, P<0.01). Conclusion: Both modified Buyang Huanwutang and atorvastatin have an effect in preventing the development of atherosclerosis.The mechanism could be related to intervention of TLR4 and its main components of downstream signal transduction pathway.关键词：Buyang Huanwutang;atherosclerosis(AS);Toll like receptor 4(TLR4);myeloid differentiation factor 88(MyD88);tumor necrosis factor receptor associated factor-6(TRAF-6);nuclear factor kappa B(NF-κB)
- 4
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe and evaluate the clinical efficacy of Xiangsha Liujunzi Tang combined with acupuncture therapy in the treatment of syndrome of deficiency of spleen Qi and stomach Qi of functional dyspepsia and investigate its effect on motilin on plasma (MOT) and serum gastrin (GAS) levels. Method: The 120 cases of patients with syndrome of deficiency of spleen Qi and stomach Qi of functional dyspepsia from January 2014 to August 2016 in Department of Gastroenterology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine were selected as research subjects and were randomly divided into treatment group (62 cases) and control group (58 cases).The patients in treatment group received oral Xiangsha Liujunzi Tang combined with acupuncture therapy, and the patients in the control group were treated with itopride hydrochloride dispersible tablets combined with compound azimtamide enteric-coated tablets treatment.Both groups were treated for 2 courses, 2 weeks/course.After the end of treatment, the patients with clinical recovery were followed up for 2 months.The two groups were observed and compared in traditional Chinese medicine(TCM) syndrome scores, the overall curative effect and the changes of plasma MOT and serum GAS levels, the Hamilton Anxiety Scale (HAMA) score and the 17 item Hamilton Depression Rating Scale (HAMD-17) score, adverse reaction and long-term curative effect. Result: After the treatment, TCM syndrome scores, HAMA scores and HAMD-17 scores in the treatment group were significantly lower than those in control group (P<0.05); the total effective rate of the treatment group was 88.71%, significantly higher than 74.14% in control group (P<0.05); the plasma MOT level was significantly higher than that of control group (P<0.05), and the serum GAS level was significantly lower than that of control group (P<0.05).No obvious adverse reactions were found during the period of treatment in two groups; the relapse rate was 11.11% in treatment group, significantly lower than 44.44% in control group (P<0.05). Conclusion: The curative effect of Xiangsha Liujunzi Tang combined with acupuncture therapy in the treatment of syndrome of deficiency of spleen Qi and stomach Qi of functional dyspepsia can significantly increase the clinical efficacy, safety,improve the anxiety and depression of the patients, and can improve the long-term curative effect.Its mechanism may be related to increasing the level of plasma MOT and decreasing the levels of GAS.关键词：functional dyspepsia;syndrome of deficiency of spleen Qi and stomach Qi;Xiangsha Liujunzi Tang;acupuncture therapy;motilin;gastrin;clinical efficacy
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Qibai granules on antioxidation and sex hormone levels for the patients with female chloasma Qi stagnation and blood stasis syndrome. Method: The 126 cases of patients with female chloasma Qi stagnation and blood stasis syndrome were divided into observation group (66 cases) and control group (60 cases).Patients in observation group were treated by Qibai granules, while patients in control group were treated by hydroquinone cream.The clinical effects were observed and compared between two groups. Result: The scores of skin lesions for observation group were lower than those in control group at week 8(P<0.05).The total effective rate and total satisfaction for observation group were higher than those in control group (P<0.05).The levels of superoxide dismutase (SOD), cata-lase (CAT) for observation group were higher than those in control group (P<0.05).The levels of malondialdehyde (MDA), E2, follicle-stimulating hormone (FSH), luteinizing hormone (LH) for observation group were lower than those in control group (P<0.05).There was no significant difference in safety classification between two groups.The scores of WHO Quality of Life Measure-Abbreviated version (WHOQOL-BREF) for observation group were higher than those in control group after treatment (P<0.05).There was no significant difference on the adverse reactions, and both groups showed no obvious abnormalities in results of blood routine, urine routine, stool routine, liver and kidney function tests. Conclusion: Qibai granules had good clinical effects for patients with female chloasma Qi stagnation and blood stasis syndrome; it can improve the skin lesions, the levels of sex hormone and quality of life, with obvious antioxidant effect, high safety, without obvious adverse reactions or significant effects on blood routine, urine routine, stool routine, liver and kidney function results, so it can be used as an optimal treatment plan.关键词：chloasma;Qi stagnation and blood stasis syndrome;Qibai granule;antioxidation;sex hormone
- 2
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of modified Bazhengsan on time of urination and ability restoration of self urination in patients with damp-heat pouring downward syndrome urinary retention after anal fistula surgery, and explore its action mechanism. Method: The 106 patients with urinary retention after anal fistula surgery were randomly divided into 2 groups, 53 cases in control group and 53 cases in treatment group.Patients in two groups were given with basic treatment such as listening to the sound of water induction, psychological counseling and bladder area massage therapy.Then patients in control group received neostigmine bromide by oral administration, and patients in treatment group received modified Bazhengsan.Urinary dynamics were detected; bladder function and the main traditional Chinese medicine (TCM) symptom scores were evaluated; urination situation was recorded and compared after the treatment. Result: Urodynamic parameters such as maximum urination quantity (Q-max) and detrusor pressure at maximum urine rate Pdet-Q-max were increased in both groups after treatment (P<0.01); maximum cysto-metric capacity (VMCC) and postvoiding residual volume (PRV) were decreased (P<0.01); bladder detrusor dysfunction, low bladder compliance and incidence of bladder sensory were decreased (P<0.05); acraturesis, abdominal bulge and languid symptom scores were decreased in both groups after treatment (P<0.01).As compared with control group after treatment, levels of Q-max and Pdet-Q-max were higher (P<0.01); VMCC, PVR levels were lower (P<0.01); the incidences of bladder detrusor dysfunction, bladder compliance and bladder sensory loss were lower (P<0.05); self urination, completely unobstructed urination, defecation and eating time was shorter (P<0.01); acraturesis, abdominal bulge and fatigue symptom scores were lower (P<0.01); and the total efficiency was higher in treatment group (P<0.05). Conclusion: Modified Bazhengsan can promote the bladder urination, reduce the residual urine volume, shorten the self urination time, and improve the ability restoration of automatic micturition, so it can be used to effectively treat damp-heat pouring downward syndrome postoperative urinary retention.关键词：after anal fistula surgery;damp-heat pouring downward syndrome;urinary retention;Bazhengsan;voiding ability
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Sanhuanggao external applying in the treatment of superficial soft tissue infection, and explore its mechanism. Method: The 120 patients with superficial soft tissue infection were randomly divided into 3 groups:high dose Sanhuanggao group, low dose Sanhuanggao group and ichthammol ointment group.The changes of local swelling, redness, pain, induration, temperature, pustular or ulcer were observed; the numerical value of blood routine, urine routine, and the indexes of liver and kidney function, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (Cr) in blood were recorded before and after 1,3,5,7th days. Result: As compared with ichthammol ointment group, Sanhuanggao external applying significantly shortened the course of disease.The course of disease was (6.93±2.74) d in large dose Sanhuanggao group, (7.75±2.93) d in small dose group, and (8.77±2.82) d in ichthammol ointment group (P< 0.05).The external application of Sanhuanggao decreased the comprehensive score of the general situation significantly (P< 0.05), promote the local mass dissipation, remove swelling, relieve pain, reduce the local temperature, and promote pustules and ulcer healing.The numbers of people whose blood and urine routine indexes as well as the liver and kidney functions (ALT, AST, BUN, Cr) returned to normal in large and small dosage Sanhuanggao groups were higher significantly than those in the control group after treatment on day 1 and day 3(P< 0.05); on day 5 and day 7 after treatment, the test results basically returned to normal, without obvious adverse reactions during treatment. Conclusion: Sanhuanggao has definite clinical efficacy in treatment of superficial soft tissue infection, which can shorten the course of disease with high clinical safety, so it is worthy of clinical promotion.关键词：Sanhuanggao;external applying;superficial soft tissue infection;alanine aminotransferase (ALT);aspartate aminotransferase (AST);urea nitrogen (BUN);creatinine (Cr)
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Shegan Mahuangtang and its effect on interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), high sensitive-C reaction protein (hs-CRP), osteopontin (OPN) and tissue inhibitors of matrix metalloproteinases-1 (TIMP-1) in acute asthmatic childhood. Method: The 161 children in acute exacerbation of bronchial asthma treated in our hospital from November 2014 to April 2016 were selected and randomly divided into two groups according to the admitted order, with 81 cases in treatment group and 80 cases in control group.Patients in control group were treated with the routine western medicine therapy, while patients in treatment group were treated with Shegan Mahuangtang which was decocted by clear water, one dose per day.Then their clinical efficacy and effects on serum cytokines, OPN and TIMP-1 in acute asthmatic childhood were compared between two groups; in addition, adverse reactions were observed in two groups. Result: The total effective rate was 93.83% in treatment group, higher than 81.25% in control group (P<0.05).The levels of IL-6, IL-8, TNF-α and hs-CRP in serum were decreased after treatment in both groups (P<0.05), and the above levels in treatment were lower than those in control group after treatment (P<0.05).The expression levels of OPN and TIMP-1 were also decreased in both groups after treatment (P<0.05), and the levels in treatment group were lower than those in control group (P<0.05).Traditional Chinese medicine (TCM) syndrome scores were significantly improved in both groups (P<0.05), and the TCM syndrome scores in treatment group were lower than those in control group after treatment (P<0.05).In addition, there was no statistically significant difference in adverse reactions between two groups. Conclusion: Shegan Mahuangtang is very effective in treating children in acute exacerbation of bronchial asthma.It can significantly lower the value of serum cytokines, OPN and TIMP-1, improve the TCM syndrome score, and the mechanism may be related to decreasing the levels of cytokines and reducing the expression levels of OPN and TIMP-1.关键词：Shegan Mahuangtang;bronchial asthma;serum cytokines;osteopontin (OPN);tissue inhibitors of matrix metalloproteinases-1 (TIMP-1)
- 2
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Dongbing Xiazhi Sanfu plaster on lung-kidney deficiency syndrome in remission stage of bronchial asthma, and investigate its mechanism. Method: From January 2013 to June 2014, 180 patients with bronchial asthma in remission stage in our hospital were collected and then were randomly divided into experimental group and control group, 90 cases in each group.Patients in control group received conventional western medicine treatment such as symbicort turbuhaler, and patients in experimental group received Dongbing Xiazhi Sanfu plaster on basis of treatment in control group for three years.The changes of symptom scores, pulmonary function before and after treatment, and the recurrence rate were observed. Result: The plaster fell off in one case of each group during follow-up period.The total effective rate was 94.38% in treatment group, and 75.28% in control group (P<0.05).Two years later, the forced expiratory volume in one second (FEV1), FEV1%, peak expiratory flow (PEF) were improved in both groups, and the improvement of PEF in experimental group was better than that in control group (P<0.05).Three years later, the three indicators were improved significantly in both groups (P<0.05), and the number of acute attacks in observation group was significantly lower than that in control group (P<0.05).Safety testing showed that twenty-one patients in experimental group had skin blisters, but they were absorbed after local treatment, with no impact on next acupoint application, and there was no serious adverse events. Conclusion: Dongbing Xiazhi Sanfu plaster can significantly reduce the symptoms during the onset in patients with bronchial asthma, such as asthma, chest tightness and cough, improve the lung function, reduce the number of asthma attacks, and improve the cure rate without serious adverse events.It indicates that Dongbing Xiazhi Sanfu plaster can improve the prognosis of bronchial asthma, worthy of widespread clinical application.关键词：Dongbing Xiazhi Sanfu plaster;bronchial asthma;forced expiratory volume in one second (FEV1);FEV1%;peak expiratory flow (PEF)
- 4
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Maxin Shigan Tang combined with Weijingtang in treating elderly patients with aspiration pneumonia with phlegm-heat stagnating. Method: A total of 120 patients were randomly divided into treatment group and control group, with 60 cases in each group.Patients in both groups received cefoperazone sodium and sulbactam sodium for intravenous infusion, 3 g/time, 2 times/day.In addition to the therapy, patients in control group received Filike mixture, 15 mL/time, tid; while patients in treatment group received Maxin Shigan Tang combined with Weijingtang, 1 dose/day.The treatment course was 14 days.Clinical efficacy, scores of traditional Chinese medicine(TCM) primary symptom, peripheral blood inflammatory marker white blood cells, C-reactive protein, calcitonin, recovery time for main symptoms and signs, average length of hospital stay were evaluated and compared. Result: The treatment group's total effective rate was 95.0%, whereas the control group's total effective rate was 81.7%, indicating that treatment group was superior to control group.Compared with control group, clinical efficacy, scores of TCM primary symptom in treatment group were higher (P<0.05).After treatment, temperature recovery time, recovery time for coughing and shortness of breath, rale disappearance time and average hospital stay were statistically significantly different between two groups (P<0.05, P<0.01), and the treatment group was superior to control group.Levels of white blood cells, C-reactive protein, calcitonin, tumor necrosis factor-α, and interleukin-6 were lower than those in control group after treatment (P<0.05, P<0.01). Conclusion: Maxin Shigan Tang combined with Weijingtang have a curative effect in treating aspiration pneumonia of elderly patients, and can relieve symptoms and signs, and shorten the course of disease.关键词：aspiration pneumonia;phlegm-heat stagnating in lung;Maxin Shigan Tang;Weijingtang;therapeutic effect
- 2
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical efficacy of iontophoresis therapy of Fuzitang combined with Shaoyao Gancaotang on non-specific low back pain (NLBP)cold dampness and blood stasis syndrome and investigate its effects on levels of interleukin-1β (IL-1β), thromboxane 2 (TXB2), 6-keto prostaglandin Flα(6-Keto-PGFlα), calcitonin gene related peptide (CGRP), and vasoactive intestinal peptide (VIP). Method: One hundred and seventy-six patients were randomly divided into control group and observation group by random number table.Patients in control group received Yaotuitong pills+electroacupuncture, and patients in observation group received Fuzitang, Shaoyao Gancaotang combined with electroacupuncture.The treatment course was 4 weeks for both groups.Before and after treatment, scores of tenderness condition in simplified McGill pain scale (SF-MPQ) were graded, and the tenderness and pain thresholds were measured.functions were evaluated by using Japan orthopedics association (JOA) method, and patients' global impression of change (PGIC) and cold dampness and blood stasis syndrome were evaluated discussed.In addition, levels of IL-1β, TXB2, 6-Keto-PGFlα, CGRP and VIP were detected, and safety evaluation was conducted. Result: The total amelioration rate of JOA was 95.18% in observation group, higher than 83.95% in control group (χ2=5.564, P<0.05).Scores of the pain feeling, pain emotion, visual analog pain, current pain conditions and total pain score in the observation group were all lower than those in control group (P<0.01).The hardness value, tenderness and traditional Chinese medicine (TCM) syndrome scores were lower than those in in control group, but pain threshold value was higher than that in control group (P<0.05 and P<0.01).Scores of subjective symptoms, clinical symptoms, limitation of daily activities, and the total sore of JOA scale in observation group were higher than those in control group (P<0.01).In addition, the markedly improved rate was 68.67% in observation group, higher than 50.62% in control group (χ2=5.558, P<0.05), and the levels of IL-1β, TXB2, CGRP and VIP were lower than those in control group, while 6-Keto-PGFlα was higher than that in control group (P<0.01). Conclusion: Iontophoresis therapy of electroacupuncture combined with Fuzitang and modified Shaoyao Gancaotang can relieve patients' pain, promote the recovery of lumbar function and improve activity ability in the patients with non-specific low back pain with cold dampness and blood stasis syndrome.关键词：non-specific low back pain;cold dampness and blood stasis syndrome;Fuzitang;Shaoyao Gancaotang;efficacy evaluation
- 2
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Yixin Tongmai grains on adverse cardiovascular events, quality of life and ventricular remodeling of patients with Qi and blood stasis after percutaneous coronary intervention therapy for coronary heart disease, and the mechanism for inflammation. Method: One hundred and sixty patients with PCI were randomly divided into control group and observation group by random number table.The two groups were adopted got aspirin enteric-coated tablets, 100 mg/time, 1 time/day,clopidogrel hydrogen sulfate tablets, 75 mg/time, 1 time/day, atorvastatin calcium tablets, 20 mg/time, 1 time/day,metoprolol tartrate sustained-release tablets, 200 mg/time, 2 times/day, and sports rehabilitation training.Patients in observation group got Yixin Tongmai grains, 10 mg/time, 3 times/day.The course of treatment was 6 months.Post-surgery restenosis (ISR) and improper perfusion (NR) were recorded.Echocardiography was performed, and left ventricular ejection fraction (LVEF), stroke volume (SV), left ventricular end-diastolic volume index (LVEDVI) and end-systolic diameter (LVEVI) were recorded before and after treatment.Seattle angina Rating scale (SAQ), Qi and blood stasis, six-minute walk test (6 MWT), ankle brachial index (ABI) were scored before and after treatment.And levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) and matrix metalloproteinase-9 (MMP-9) were detected before and after treatment. Result: During the 6 months, incidence of ISR in observation group was 6.58%, which was lower than 19.44% in control group (χ2=5.051, P<0.05), incidence of NR in observation group was 9.59%, which was lower than 22.22% in control group (χ2=4.334, P<0.05).After treatment, LVEF and SV were higher than those in control group (P<0.01), LVEDVI and LVEVI were lower than those in control group (P<0.01).After treatment, scores of degree of motion limitation, angina stability, angina attack and treatment satisfaction were all higher than those in control group (P<0.01).Levels of TNF-α, IL-6, NT-proBNP and MMP-9 were lower than those control group (P<0.01).The 6-minute walking distance in observation group were longer than that in control group (P<0.01), score of Qi and blood stasis in observation group was lower than that of control group (P<0.01), and ABI was higher than that in control group (P<0.01). Conclusion: After PCI, Yixin Tongmai grains can reduce ISR and NR, inhibit ventricular remodeling and inflammatory response, relieve clinical symptoms, improve ventricular function, and enhance patients' motion ability and quality of life, so as to promote recovery of patients.关键词：coronary heart disease;percutaneous coronary intervention therapy;Yixin Tongmai grains;adverse cardiovascular event;recovery;inflammatory response
- 2
- |
- 4
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: Data mining method was adopted to summarize the medication regularity in each phase and the action mechanism of infertility with traditional Chinese medicine(TCM) periodic therapy. Method: Clinical research literatures of treatment for infertility by TCM periodic therapy in 1980-2016 were searched,a database of Excel was established and SPSS Clementine 12.0 software was used to analyze the data. Result: A total of 143 TCMs and 3 609 frequencies were involved in the 85 literatures.Sumed up the TCMs with high usage frequency in the prescriptions for each phase.The result preliminary proved that tonifying the kidney was used throughout the TCM periodic therapy. Conclusion: The main rules of treatment in post menstrual period are nourishing the essence and enriching the blood,in ovulation are promoting blood circulation for removing blood stasis and regulating blood vessels,in premenstrual phase are warming kidney and activating Yang,in menstrual period are promoting blood circulation and removing blood stasis for promoting tissue regeneration.The key point of TCM periodic therapy for infertility is regulating Tiangui in the kidney.关键词：infertility;traditional Chinese medicine periodic therapy;medication regularity;action mechanism;Tiangui
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Berberine, extracted from Coptis chinensis, Phellodendron amurense and other herbs, is a kind of alkaloid with significant antibacterial effects.Ithas been used in the treatment of bacterial gastroenteritis, dysentery and other gastrointestinal diseases for a long time.In recent years, other pharmacological activities of berberine have beengradually discovered.The studies have shown that berberine has significant effects on the cardiovascular system, nervous system, endocrine system, especially effective for the prevention and treatment of atherosclerosis, and berberine can also increase the sensitivity of insulin to treat diabetes.In addition, it is found that berberine has anti-inflammatory actions and anti-cancer effects.Berberine can kill tumor cells, inhibit proliferation of tumor cells and induce apoptosis of tumor cells.Berberine can also affect the ion channels of biomembrane such as calcium ion and potassium ion to adjust the electrolyte balance.Because of the anti-inflammatory and anti-oxidation actions, it is also used to treat peptic ulcer.In this report, the actions and mechanism of berberine were summed up,including the effects of berberine on the cardiovascular system, nervous system, endocrine system and the pharmacological effects of anti-inflammatory, anti-bacterial, and anti-cancer actions, as well as its effects onion channels, cyclin, and extracellular matrix.The actions and mechanism of berberine have obtained more and more attention, especially anti-cancer effects, indicating there will be new progress in the study on traditional Chinese medicines and prescriptions containing berberine.The summary of actions and mechanism of berberine would be helpful to improve the clinical applicationsof berberineand R&D of new drugs.关键词：berberine;pharmacological action;mechanism;antibacterial
- 3
- |
- 3
- |
- 26
发布时间：2024-01-04 - 摘要：With the increasing scarcity of Cordyceps sinensis, fermented C.sinensis powder that produced through extraction and separation of medical strains from C.sinensis by artificial fermentation has gradually become the substitutes of C.sinensis.Modern pharmacology had verified that the pharmacological activity of fermented C.sinensis powder is similar to that of C.sinensis, because they have similar active ingredients.Currently, there were numerous products of fermented C.sinensis powder, mainly including Jinshuibao capsule, Bailing capsule, Xinganbao capsule, Zhiling capsule, Ningxinbao capsule.Among them, only Jinshuibao capsule and Bailing capsule have reached comprehensive quality standards and been recorded in the 2015 edition of Chinese Pharmacopoeia,while others have inferiorquality standards.For the product of fermented C.sinensis, the unified quality control method has not been established.Therefore, the establishment of improved and unified quality standards for products of fermented C.sinensis powder is the key to improve its quality standard and ensure its safety, high quality and efficiency.This essay summarized the chemical composition and the progress in quality control by analyzing the deficiency of the quality standard of products of fermented C.sinensis powder, and propose to establish a more scientific and rational model of quality standard for comprehensively evaluating its quality, withthe aim to provide a certain reference for the improvement of quality standard and the standardized production of products of fermented C.sinensis powder.关键词：fermented Cordyceps sinensis powder;chemical composition;quality control
- 2
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Scutellariae Radix is the dried roots of Scutellaria baicalensis belonging to Labiataeae.It has been used as a medicine for a long time and mainly for the treatment of pulmonary cough, acute dysentery, irascibility and headache.In recent years, scholars at home and abroad have found that baicalin is one of the active ingredients contained in Scutellariae Radix, with anti-bacterial,anti-inflammatory, sedationand reducing blood pressure, protecting liver and gallbladder and other pharmacological effects.Magnesium, as one of the necessary elements that human body needs, plays an important role in the metabolism of the human body.This paper was to study the pharmacological effects and drug-preparations of baicalin and magnesium byreviewing relevant references in Wanfang, CNKI and PubMed databaseswithinrecent years.It was foundthat in addition toanti-inflammatory, anti-virus and anti-oxidation effects, baicalin was alsoeffective in the treatment of hepatitis and asthma, with anti-tumor andanti-human immunodeficiency virus(HIV)-1 activities, and it could promote the growth of embryonic in vitro.In addition, it also had protective effects on cerebral ischemia-reperfusion injury and diabetic nephropathy.However, the pharmacokinetic study of baicalin showed that it had poor water solubility and low absolute bioavailability.Researchers developed a number of new drug delivery systems to improve the bioavailability of baicalin.Previous studies had confirmed that the original form of baicalin in Scutellariae Radix was baicalin magnesium salt.In order to explore the possible pharmacological action of baicalin magnesium salt and the feasibility of its preparations, pharmacological effects and mechanism of baicalin and magnesium were summarized brieflyin this paper, and the future directions for baicalin magnesium salt pharmacological study and drug-preparations wereproposed, hoping toprovide references for the studyof baicalin magnesium salt and lay a foundation foritsdevelopment and clinical applications.关键词：baicalin;magnesium;baicalin magnesium salt;pharmacological effects
- 2
- |
- 3
- |
- 12
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0