最新刊期
卷 23 ， 期 4 ， 2017
- 摘要：Objective: To get Eucommiae Cortex and salt-processed product of Eucommiae Cortex decoction pieces powder with optimum homogeneity by screening crushing process,in order to make them to be the standard reference materials. Method: According to microexamination and particle size distribution of Eucommiae Cortex and salt-processed product of Eucommiae Cortex decoction pieces in different grinding manners and the particle size distribution of control medicinal powder,ultrafine grinding was optimum grinding manner of them.Homogeneity of decoction pieces was compared by means of spectral difference,color and content of active ingredient. Result: Eucommiae Cortex and salt-processed product of Eucommiae Cortex decoction pieces powder was fine and had good fluidity when ultrafine grinding of 30 min,the spectral deviations of them were 0.002 and 0.001,RSDs of total color value were 0.5% and 0.4%,RSDs of pinoresinol diglucoside content of them were 2.3%. Conclusion: Ultrafine grinding of 30 min is the best grinding technology for Eucommiae Cortex and salt-processed product of Eucommiae Cortex decoction pieces,it provides a reference for formulating homogenization specifications of them.关键词：Eucommiae Cortex;salt-processed product of Eucommiae Cortex;homogenization;ultrafine grinding;near-infrared;pinoresinol diglucoside;control medicinal herbs
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发布时间：2024-01-04 - 摘要：Objective: To prepare silymarin nanocrystal pellets and rsearch for its characterization and stability test. Method: Silymarin nanocrystal pellets were prepared by fluid-bed spray drying technology and characterized by laser particle size analysis,ultraviolet spectroscopy (UV),infrared spectroscopy (FT-IR),differential thermal analysis (DSC) and X-ray diffraction (XRD).Preliminary stability were investigated (high temperature,high humidity and light test). Result: Average particle size of silymarin nanocrystal pellets was (251.6±3.8) nm,and the polydispersity index (PDI) was 0.184±0.015.The chemical structure of silymarin did not change by the analysis of UV and FT-IR.Silymarin was dispersed in carrier with an amorphous or molecular state.Under the conditions of 60℃,relative humidity of 75% and light irradiation of (4 500±500) Lux,the appearance,redispersion,content of silymarin and dissolution rate of nanocrystal pellets were not changed obviously. Conclusion: Silymarin nanocrystal pellets offers rapid drug release and good stability,which is expected to be further developed into oral solid preparations.关键词：silymarin;nanosuspensions;nanocrystal pellets;polydispersity index;characterization;dissolution;stability
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to investigate the penetration difference between paeoniflorin gel and paeoniflorin-ethosome gel after dermal application,and then identify the penetration form of paeoniflorin. Method: Improved Franz diffusion cell method were used in the transdermal delivery experiment with rat abdominal skin as in vitro model;and penetration rates,retention amounts on the dermal surface of paeoniflorin in two formulations were investigated.The amount of paeoniflorin in percutaneous receptor fluid and residual drugs were determined by HPLC. Result: The cumulative amount of penetration in paeoniflorin-ethosome gel was 1.76 times bigger than that in paeoniflorin gel and the percutaneous percent was 2.03 times bigger.Both percutaneous results were consistent with zero-level model.Parts of the paeoniflorin-ethosome permeated the skin was in an integrity structure. Conclusion: Because of its deformability and cutaneous integration,ethosome can enhance the permeability of paeoniflorin.关键词：paeoniflorin;ethosome;transdermal penetration;transdermal mechanism of permeation;gel
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发布时间：2024-01-04 - 摘要：Objective: A comparison was made between fingerprint spectrum of urine-reducing part of Suoquan Wan constituted with non-processed and salt-processed Alpiniae Oxyphyllae Fructus fruits to discuss the chemical composition changes after salt-processing that enhance the urine-reducing effect. Method: HPLC was used for analysis with mobile phase of acetonitrile-water for gradient elution and detection wavelength at 230 nm.HPLC spectra of 10 batches Suoquan Wan constituted with non-processed and salt-processed Alpiniae Oxyphyllae Fructus fruits were recorded.Establishing the fingerprint spectra,32 peaks marked on each fingerprint spectrum had been compared. Result: The average relative area of 11 common peaks increased after salt-processing of Alpiniae Oxyphyllae Fructus fruits.Chromatographic peaks of No.1,No.4 (teuhetenone A),No.5 (7-epi-teucrenone B) and No.7 were the major contribution to urine-reducing effect,which increased 123.08%,90.91%,53.33% and 43.75%,respectively. Conclusion: The change of those 4 components maybe closely related to the enhancement of urine-reducing effect of Suoquan Wan,and the change of other 7 components may be synergistic to it.关键词：Suoquan Wan;Alpiniae Oxyphyllae Fructus fruits;salt-processing;fingerprint spectrum;urine-reducing part;nootkatone
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Massa Medicata Fermentata composed by fresh or dry Chinese medicines and prepared by different preparation methods on gastric emptying,intestine propulsion and regulating intestinal flora of dyspepsia mice. Method: Four groups of Massa Medicata Fermentata samples were prepared,the first group is the basic one composed of flour,rice bean and bitter almond,the fresh product groups (the basic group and fresh medicine juicing or decocting) and the dry product group (the basic group and dry medicine decocting).Mice were used to set up dyspepsia and gastrointestinal disorders model by ig 100% refine lard oil with dose of 0.4 mL·g-1 for 10 d.Experimental animals were divided into 6 groups (the blank group,the model group and four sample groups).Sample groups were given Massa Medicata Fermentata suspension by oral administration with 0.2 mL·g-1,the blank group and the model group were administrated equivalent distilled water,once a day and for seven consecutive days.Impact of sample groups on gastric emptying rate and intestine propulsion rate was observed.Flora condition in colonic contents was detected by polymerase chain reaction technique (PCR). Result: In the gastrointestinal dynamics experiment,compared with the model group,each drug administration group could promote the movement of gastric emptying and intestinal propulsion,but their levels were different.In the detection of intestinal flora,each drug administration group could raise significantly the growth of intestinal beneficial bacteria (such as lactobacillus,bifidobacterium and bacteroides) and their levels were different. Conclusion: Massa Medicata Fermentata composed of fresh Chinese medicines can promote stomach intestine movement significantly and regulate the intestinal flora,both experiments led to corresponding results together.Massa Medicata Fermentata composed of fresh Chinese medicines is better than dry ones.The principles of formulating prescription about Massa Medicata Fermentata is fresh medicine decocting for the best.The spleen function of Massa Medicata Fermentata is concerned with promoting gastrointestinal,adjusting the intestinal flora and promoting the growth of bacteria.关键词：Massa Medicata Fermentata;fresh Chinese medicines;dyspepsia mice;gastric emptying;intestine propulsion;intestinal flora
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发布时间：2024-01-04 - 摘要：Objective: To compare the inhibition effect of raw and rice-wine products of Trigonellae Semen on α-glucosidase and α-amylase for screening of effective fraction with inhibiting activity.Then try to analyze its reaction kinetics. Method: Four different polar fractions,including petroleum ether fraction,ethyl acetate fraction,n-butanol fraction and water fraction,were extracted from 80% methanol extract of raw and rice-wine products of Trigonellae Semen.α-Glucosidase inhibitory model and α-amylase inhibition model in vitro were adopted to measure inhibition effect of different polar fractions on α-glucosidase and α-amylase.Then their inhibitory types were inferred by enzymatic dynamics and Lineweaver-Burk curve methods. Result: The water fraction from raw and rice-wine products of Trigonellae Semen had inhibition effect on α-glucosidase with non-competitive and reversible type,the rice-wine product of Trigonellae Semen was better than raw product.The ethyl acetate and n-butanol fraction from raw and rice-wine products of Trigonellae Semen had inhibition effect on α-amylase with non-competitive and reversible type,and the rice-wine product of Trigonellae Semen was better than raw product. Conclusion: The inhibition effect of Trigonellae Semen on α-glucosidase and α-amylase can be increased after being processed by rice-wine.The water fraction from rice-wine product has medicinal development values as a new α-glucosidase inhibitor,the ethyl acetate and n-butanol fraction from rice-wine product has medicinal development values as new α-amylase inhibitors.关键词：Trigonellae Semen;rice-wine product;enzyme activity;α-glucosidase;α-amylase;extraction site;enzyme reaction kinetics
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发布时间：2024-01-04 - 摘要：Objective: To dicuss processing mechanism of charred Rehmanniae Radix Praeparata with nourishing blood and hemostatic effect by screening its main active site. Method: Effective parts of charred Rehmanniae Radix Praeparata for hemostatic efficacy were screened with bleeding time (BT) and clotting time (CT) as indexes.Taking SD rats as the research object,by intraperitoneal injection of cyclophosphamide combined with tail bloodletting,and with 8% ethanol instead of free drinking water to establish rat model with blood deficiency and bleeding,the general physical signs and pathological tissue were observed,red blood cell count (RBC),hemoglobin content (HGB) and white blood cell count (WBC) were determined;hemorrheological parameters and blood clotting tetrachoric[prothrombin time (PT),thrombin time (TT),activated partial thromboplastin time (APTT) and plasma fibrinogen (FIB)] were measured. Result: Compared with the blank group,charred Rehmanniae Radix Praeparata and its water part could shorten BT and CT of mice.Compared with the blank group,RBC,HGB and WBC of blood deficiency and bleeding model group rats decreased significantly;whole blood reduced viscosity under high and low shear increased;PT,TT and APTT prolonged and FIB increased.Compared with the model group,RBC,HGB and WBC of rats in the charred Rehmanniae Radix Praeparata group increased significantly;whole blood reducted viscosity and plasma viscosity under high,medium and low shear were decreased;PT,TT and APTT were shortened and FIB was reduced.Compared with the model group,RBC and HGB of rats in water part group increased;whole blood reduced viscosity under high shear was decreased;PT,TT and APTT were shortened and FIB was reduced. Conclusion: Charred Rehmanniae Radix Praeparata has a good nourishing blood and hemostatic effect for rat model with blood deficiency and bleeding.关键词：charred Rehmanniae Radix Praeparata;nourishing blood and hemostatic effect;blood deficiency and bleeding;processing mechanism;thrombin time;white blood cell count
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发布时间：2024-01-04 - 摘要：Objective: To optimize supercritical CO2 fluid extraction process of volatile oil from Angelicae Sinensis Radix and provide reference for its further research and development. Method: UPLC was adopted to determine the contents of coniferyl ferulate and ligustilide in volatile oil from Angelicae Sinensis Radix with mobile phase of acetonitrile-water (50:50),detection wavelength at 320 nm and the flow rate of 0.4 mL·min-1.With extracting amounts of coniferyl ferulate and ligustilide as indexes,extraction parameters including temperature,pressure and time were optimized by orthogonal test. Result: Coniferyl ferulate and ligustilide showed good linearity at 0.006 2-0.777 6 μg and 0.017 9-2.238 μg,respectively;their recoveries were between 96.67%-102.08%.Optimum extraction parameters were as follows:extraction temperature at 40℃,extraction pressure of 25 MPa and extraction time of 2 h;average extracting amounts of coniferyl ferulate and ligustilide were 1.62,10.28 mg·g-1,respectively. Conclusion: This UPLC is repeatable and simple,it can be used for quality control of volatile oil from Angelicae Sinensis Radix.Optimum extraction process is stable and feasible for extraction of volatile oil from Angelicae Sinensis Radix.关键词：Angelicae Sinensis Radix;supercritical CO2 fluid extraction;Coniferyl ferulate;ligustilide
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of polysaccharides from Abelmoschus esculentus leaves by response surface methodology and investigate its in vitro antioxidant activity, in order to provide reference for developing A.esculentus leaves. Method: Based on single-factor experiments, three independent factors, namely ultrasonic power, extraction temperature and extraction time, were selected as affecting factors for Box-Behnken central composite experiment design. With the polysaccharide extraction rate as the response value, the response surface analysis method was applied to optimize the polysaccharide extraction technique of A.esculentus leaves. DPPH·and·OH scavenging assays were carried out to study in vitro antioxidant activity of polysaccharides from A.esculentus leaves. Result: The results showed that the optimum conditions for extracting polysaccharides from A.esculentus leaves were ultrasonic power at 160 W, extraction temperature at 54℃, extraction time of 91 min. Under these conditions, the extraction rate of polysaccharides was 4.81%, with a small deviation from the theoretical value. The half inhibiting concentrations (IC50) to DPPH·and·OH were 2.65, 1.47 g·L-1 respectively. Conclusion: The optimized extraction technique is stable and feasible, with a high extraction rate. Polysaccharides from A.esculentus leaves have a better anti-oxidation activity, and provide reference for industrialized production of A.esculentus.关键词：Abelmoschus esculentus leave;polysaccharide;ultrasonic;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To develope a dynamic optimization technique (DOT) method for the extraction of ginsenosides from ginseng slices. Method: The contents of three major ginsenosides (Rg1, Re and Rb1) were determined by HPLC. The extraction yield, contents of ginsenosides and fingerprints similarity were compared between the DOT method and literature extraction process, to evaluate the feasibility of DOT in optimizing the extraction of ginsenosides from ginseng slices. Result: The dynamic optimization process was extracting for thrice with 8 times by 70% ethanol, and extracting time was 40, 20, and 40 minutes in turn with a total extract yield of 41.3 mg·g-1. The extract between DOT process and the literature extraction process was basically the same, but the DOT extraction method could save 63% of extraction time and 60% of power consumption. Conclusion: DOT extraction method can provide shorter extraction time and lower energy cost in the industrial production of ginsenosides from Ginsen Radix et Rhizoma, and it can produce obvious economic value.关键词：dynamic optimization method;literature extraction process;Ginseng Radix et Rhizoma;ginsenosides;extraction time
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发布时间：2024-01-04 - 摘要：Objective: High quality seedling is the foundation to ensure the quality and yield of medicinal herbs. It is meaningful to find agreen, safe and effective measures to shorten the time of germination, improve the germination rate and germination uniformity. In this paper, the seeds of 5 species of Chinese medicinal herbs are used as research materials. Compared with common growth regulators on Seed Germination. Method: Smoke water(SW) was made from Plane tree; TTC was used to measure the seed vigor; measure the moisture content of the seeds. The water uptake rate of the seeds was detected. Seeds were treated by water (control group),SW (0.2% solution) and plant growth regulators,such as gibberellin (150 mg·L-1), lanthanum chloride (0.6 mg·L-1), calcium nitrate (30 mg·L-1) and salicylic acid (0.028 mg·L-1). Comparison the effects of smoke water and four plant growth regulators on seed germination rate/mean germination time/germination index of five highly traded Chinese medicine plants(Isatis indigotica,Platycodonis Radix,Anemarrhenae Rhizoma,Scutellariae Radix,Astragali Radix). Result: SW showed a significant improve effect to the germination rate of I. indigotica,Platycodonis Radix,Scutellariae Radix, it significantly promote the germination rate of I. indigotica for 27.27%.It showed no apparent influence on the mean germination time of the seeds.Significantly promote the germination index of Platycodonis Radix and Scutellariae Radix for 32.71% and 27.73%. It promote seed germination similar with gibberellin(GA3). Conclusion: SW is non-toxic and wild available, these characteristics make it more suitable for agricultural practice. SW has great potential in promoting seed germination of Chinese medicinal plants.关键词：smoke water;plant growth regulator;germination
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发布时间：2024-01-04 - 摘要：Objective: To provide basis for utilization and development of germplasm resources of Rosae Laevigatae Fructus. Method: Totally 20 germplasms of Rosae Laevigatae Fructus were collected from Zhejiang, Chongqing, Hunan, Shanxi, Guangdong, Anhui and Jiangxi provinces of China. Subsequently, longitude and latitude, elevation and agronomic traits were recorded and measured, while polysaccharose contents were measured by phenol-sulfuric acid method. The data obtained were analyzed by SPSS 17.0 and DPS 9.05 software with ANOVA and UPGMA methods respectively. Result: There were significant differences in agronomic traits and polysaccharose contents among Rosae Laevigatae Fructus from different origins (P<0.01), and polysaccharose content of germplasm in Zhejiang was relatively high. Meanwhile, there were variation rules between polysaccharose contents as well as agronomic traits and biotope factor. In particular, there were significantly positive relations between polysaccharose content as well as ratio of length and width of leaves, ratio of vertical and lateral diameter of fruit and longitude, with negative relations with lateral diameter of fruit, and low relations with weight of fruit and fruit fresh, and latitude. What's more, 10 characters were simplified to 4 principle components, with the accumulative contribution rate up to 94.69%. Besides, 20 germplasms were divided into 5 groups. Among them, the first group showed a higher ratio of length and width of leaves and ratio of vertical and lateral diameter of fruit, with a higher polysaccharose contents. The fourth and fifth groups showed a broad and thick leave area, low ratio of length and width of leaves, large fruit and higher ratio of vertical and lateral diameter of fruit. Conclusion: Five germplasms with superior agronomic traits and four germplasms with higher polysaccharose content have been selected, and could be used for breeding high-yield and high-quality Rosae Laevigatae Fructus.关键词：Rosae Laevigatae Fructus;polysaccharose;variation rules;superior germplasm
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发布时间：2024-01-04 - 摘要：Objective: To optimize the denaturing gradient gel electrophoresis(DGGE) experimental methods applicable to analyze the community composition of phylloplane bacteria and fungi sampled from Aconitum carmichaeli. Method: Touch Down-PCR experiment was conducted to amplified A. carmichaeli phylloplane bacteria 16 S rDNA V3 area and fungi 18 S rDNA~5.8 S rDNA sequences, in order to optimize DGGE electrophoresis conditions. DGGE vertical electrophoresis was used to ameliorate degeneration gradient of gels, and time interval method was used to figure out electrophoresis time. Result: For the phylloplane bacteria, the optimum analytical conditions were denaturant gradient ranging from 30% to 65%(Gel 10%), electrophoresis temperature at 60℃, voltage of 120 V and electrophoresis time of 7 h. For phylloplane fungi, the optimum analytical conditions were denaturant gradient range ranging from 25% to 45%(Gel 8%), electrophoresis temperature at 60℃, voltage of 120 V and electrophoresis time of 10 h. Conclusion: The above DGGE analysis conditions can be used to effectively distinguish A. carmichaeli phylloplane bacteria 16 S rDNA V3 area and fungi 18 S rDNA-5.8 S rDNA sequences, so as to provide reliable support for PCR-DGGE analysis of community structure of A. carmichaeli phylloplane bacteria and fungi.关键词：Aconitum carmichaeli;phylloplane;bacteria;Fungi;denaturing gradient gel electrophoresis
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the ethyl acetate extract of Lonicerae Japonicae Caulis made in Shandong. Method: Lonicerae Japonicae Caulis made in Shandong was crushed into coarse powder, extracted by 70% ethanol, concentrated under reduced pressure, and then its ethyl acetate extract was gained by fractional extraction. Chemical compounds were isolated and purified by using repeated column chromatographies such as silica gel, MCI-gel resin, and Sephadex LH-20.Their structures were elucidated by spectral data and physicochemical properties. Result: Ten compounds were obtained and identified as syringic acid (1), ursolic acid (2), pinoresinol (3),oleanolic acid (4), diosmetin (5), β-sitosterol (6), cinnamic acid (7), apigenin (8), luteolin (9), and quercetin (10). Conclusion: Compounds 1-3 were isolated from Lonicera for the first time, and compounds 4-5 were isolated from Lonicerae Japonicae Caulis for the first time.关键词：Lonicerae Japonicae Caulis;ethyl acetate extract;syringic acid;ursolic acid;pinoresinol
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发布时间：2024-01-04 - 摘要：Objective: To separate the chemical compositions of root of Cynara scolymus and identify the structures of the monomer compounds. Method: 90% and 60% ethanol extracts were respectively used to extract root of C. scolymus and obtained the ethanol extraction of this plant, then they were extracted for 3 times with petroleum ether, ethyl acetate, and n-butanol to get three different polarity extractions. The extractions were dried to get three liquidum extractum. The ethyl acetate liquidum extractum were isolated and purified with repeated silica gel column chromatography, D-101 macroporous adsorption resin, ODS reversed phase column chromatography, hydroxypropyl sephadex column chromatography, high performance liquid chromatography, high-performance preparative liquid chromatography, and continuous recrystallization. The structures were identified on the basis of spectral data and physicochemical properties. Result: 11 Compounds were isolated from root of C. scolymus and their structures were identified as ethyl caffeate (1), 5-methoxyfurfural (2), vanillic acid (3), protocatechuic aldehyde (4), 5-O-caffeoyl quinic acid methyl ester (5), apigenin (6), luteolin (7), luteolin-7-O-β-D-glucoside (8), cynarine (9) cynarine methyl ester (10) and β-sitosterol (11). Conclusion: Compounds 2, 3, and 4 were isolated from Cynara for the first time and compounds 1, 5, and 10 were isolated from root of C. scolymus for the first time.关键词：Cynara scolymus;root;5-methoxfurfural;vanillic acid;protocatechuic aldehyde
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发布时间：2024-01-04 - 摘要：Objective: To study on secondary metabolites of endophytic fungus Rhizopus sp. from Astragali Radix. Method: AR02 was isolated from Astragali Radix by the method of histology. The fungus was identified by morphological method combined with molecular biology method. Large-scale fermentation of endophytic fungi used solid culture method. The compounds were isolated by column chromatography, Sephadex LH-20, and HPLC. They were identified on the basis of physic-chemical constants and spectral analysis. Result: Endophytic fungi AR02 isolated Astragali Radix were identified as Rhizopus sp. Seven compounds were obtained and elucidated as tryptanthrin (1), lawsaritol A (2), kojic acid (3), trypacidin (4), monomethylsulochrin (5), emodin 8-O-methylether (6) and cannabifolactone A (7). Conclusion: Compounds 1-3, 6 and 7 were isolated from the endophytic fungi of Astragali Radix for the first time. The secondary metabolites of Rhizopus sp. appear in several categories and the endophytic fungus, Rhizopus sp. from Astragali Radix is a promising source for active substances.关键词：endophytic fungi;Astragali Radix;Rhizopus sp.;secondary metabolites
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发布时间：2024-01-04 - 摘要：Objective: To isolate the chemical constituents from Sedi Herba and identify the structure of the monomer compounds. Method: The 15 grams of the whole dry Sedi Herba was heated by 10 times the amount of 70% ethanol for 3 times(2 hours for each time) and the filtrate was combined to get the extracts.The extracts was mixed by 200-300 mesh silica gel and the elution was using petroleum ether-ethyl acetate(100:1-98:2-9:1-7:3) and dichloromethane-methanol(100:0-95:5-9:1-8:2-7:3-6:4) to get 10 sites.Silica gel,Sephadex LH-20,ODS,PTLC and recrystallization were used for the isolation and purification of chemical constituents of 5 sites. Their structures were identified on the basis of physico-chemical properties and spectral analysis. Result: Twelve compounds were isolated and their structures were identified as follows:δ-amyrin (1), δ-amyrione (2), 3-epi-δ-amyrin (3), β-sitosterol (4), luteolin (5), α-amyrin (6), kaempferide (7), hyperoside (8), apigenin (9), vanillic acid (10), quercitrin (11), daucosterol (12). Conclusion: Compounds 9 and 10 were isolated from this genus for the first time,compounds 6 and 7 were isolated from Sedi Herba for the first time.关键词：Sedi Herba;chemical constituents;structure identification
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Plantaginis Semen. Method: The constituents were isolated and purified by macroporous resin HP-20, column chromatography over silica gel, RP-8 silica gel, Sephadex LH-20 and by preparative HPLC. Their structures were elucidated by analysis of physical and chemical properties and spectral data. Result: Nine compounds were isolated from Plantaginis Semen and their structures were identified as cyclo-(S-Pro-R-Phe) (1), (+)-dehydrovomifoliol (2), blumenol A (3), 1H-indole-3-carbaldehyde (4), daucosterol (5), α-linoleic acid (6), α-linolenic acid (7), γ-linolenic acid (8), oleic acid (9). Conclusion: Compounds 1, 2, 4, 8 were isolated from Plantaginaceae for the first time.关键词：Plantaginis Semen;chemical constituents;cyclo-(S-Pro-R-Phe);(+)-dehydrovomifoliol;1H-indole-3-carbaldehyde;γ-linolenic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish a UPLC-MS/MS method for the content determination of dehydrotumulosic acid and pachymic acid in Poria. Method: Chromatographic separation was carried out on ZORBAX Eclipse XDB-C18 column (2.1 mm×100 mm, 1.8 μm) with acetonitrile-1.0 mmol·L-1 ammonium acetate (80:20) as the mobile phase. The flow rate was 0.3 mL·min-1, and the column temperature was 40℃. The injection volume was 5.0 μL. Electrospray ionization (ESI-) under negative ion mode was used, and the quantization of dehydrotumulosic acid and pachymic acid was performed with multiple reaction monitoring (MRM) mode. The detected ion-pairs were dehydrotumulosic acid m/z 483.20/421.20 and pachymic acid m/z 527.40/465.30. Result: The linear range was 24.2-488 μg·L-1(r=0.999 9) for dehydrotumulosic acid, and 26.2-524 μg·L-1 (r=0.999 8) for polyporenic acid. The retention time was 6 min. The average recoveries of dehydrotumulosic acid and polyporenic acid were 99.8% with RSD of 1.2% (n=6) and 101.4% with RSD of 1.2% (n=6), respectively. Conclusion: The proposed UPLC method is rapid, accurate, selective, sensitive, and suitable for the content determination of dehydrotumulosic acid and polyporenic acid in Poria.关键词：UPLC-MS/MS;Poria;polyporenic acid;dehydrotumulosic acid
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for simultaneous determination of five compounds, including three iridoid glucosides (10-acetyl-scandoside, scandoside methyl ester, and deacetylasperuloside methyl ester) and two flavonoid glucosides {quercetin-3-O-[2-O-(6-O-E-ferloyl)-β-D-glucopyranosyl]-β-D-galactopyranoside and kaempferol-3-O-[2-O-(6-O-E-ferloyl)-β-D-glucopyranosyl]-β-D-galactopyranoside} in Hedyotis diffusa injection. Method: The separation of targeted compounds was performed on SunFireTM C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution. The column temperature was 30℃ and the flow rate was 1.0 mL·min-1. Result: The method showed good linearity (r>0.999 5) within the linear range. The recoveries were in the range of 98.1%-101.2% with RSD<3.3%. Conclusion: The method is reliable, simple and reproducible, and it can be used to control the quality of Hedyotis diffusa injection.关键词：Hedyotis diffusa injection;HPLC;multi-components;simultaneous determination
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for the content determination of quercitrin in Emilia prenanthoidea. Method: The orthogonal experiment design and single factor test combined with statistical analysis had been applied to optimize the extraction conditions of E. prenanthoidea for improving the yield of quercitrin. The contents of quercitrin in E. prenanthoidea were determined by HPLC; detection was performed on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.1% acetic acid solution (20:80) as the mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 351 nm, and sample size was 10 μL. Result: The optimal extraction conditions were as follows:0.5 g medicinal powder (40 mesh), added with 20 mL 80% methanol solution, and then hot reflux extraction for 30 min one time. Quercitrin had excellent linearity within the concentration range in 5.541-177.3 mg·L-1 (r=0.999 9), and the average recovery rate was 99.87% (RSD 2.0%). Conclusion: The established HPLC method is simple, rapid, and accurate, so it can be used for the quality control of Emilia prenanthoidea.关键词：HPLC;Emilia prenanthoidea;orthogonal experiment;quercitrin
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC-fingerprint (HPLC-FP) analysis method for Elaeagnus pungens leaves and provide a new method for their quality control. Method: HPLC separation was performed on Agilent ZOTBAX XDB-Phenyl C18(4.6 mm×250 mm, 5 μm) with 0.1% phosphoric acid-acetonitrile as the mobile phase for gradient elution; the column temperature was 30℃; the flow rate was 1.0 mL·min-1; the detection wavelength was set at 360 nm. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004A edition) was used to evaluate the similarity of 12 batches of E. pungens leaves. Result: With tiliroside as the reference peak, sixteen common peaks were obtained in 12 batches of samples. The similarity of each fingerprint within one group was over 0.80, and squared Euclidean distance method was used for clustering analysis. Conclusion: This method shows high precision, stability and good reproducibility, and it can provide basis for the quality control of E. pungens leaves.关键词：Elaeagnus pungens leaves;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: According to urine metabonomics differences between spontaneously hypertensive rats (SHR) and WKY rats,to investigate effect of Danqi tablets on blood pressure and urine metabolomics of SHR. Method: SHR and WKY rats were used in the study.SHR were randomly divided into Danqi tablets group(dose of 0.38 g·kg-1),aspirin group (10 mg·kg-1),Danqi tablets+aspirin group (dose of 0.38 g·kg-1 and 10 mg·kg-1).Systolic blood pressure (SBP) of rat tail artery was constantly monitored before and after administration and rat urine in 24 hours was collected.All urine samples were detected by 1H-NMR.Partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to discussed effect of Danqi tablets and aspirin on metabolites in urine of SHR. Result: Compared with the model group,blood pressure of all groups was significantly lower after 4 weeks.Compared with WKY rats, 21 kinds of different metabolites were found in SHR urine,they mainly included alanine,succinate,dimethylamine and so on.Danqi tablets,aspirin and Danqi tablets+aspirin all can make the distribution of a part of different metabolites in SHR urine back to normal. Conclusion: Danqi tablets and Danqi tablets+aspirin can lower the blood pressure of SHR,these two kinds of drugs can adjust distribution of relevant differetial metabolites associated with hypertension.关键词：promoting blood circulation for removing blood stasis;hypertension;rats;urine;Danqi tablets;aspirin;citric acid
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发布时间：2024-01-04 - 摘要：Objective: To investigate stability of harpagoside in biological fluids in vitro and analyze its constituents absorbed in rat serum after oral administration. Method: Harpagoside was dissolved in artificial gastric juice,intestinal juice and gastrointestinal contents of rats,the corresponding pH solution,respectively;then thermostatically maintained at 37℃.HPLC was used to calculate the remaining rate of harpagoside in biological samples by measuring the content of harpagoside at time intervals after degradation.Constituents absorbed in rat serum were detected by LC-MS after oral administration. Result: Harpagoside was relatively stable in artificial intestinal juice and intestinal contents of rats,its degradation rates were less than 10%;however,it could be degraded in diluted acid,artificial gastric juice and gastric contents of rats,its degradation rates were less than 15%.Harpagoside,cinnamic acid and harpagide were detected in rat serum after oral administration of harpagoside. Conclusion: Harpagoside is relatively stable in artificial gastric juice,intestinal juice as well as gastrointestinal contents of rats,and a few of it in the acidic environment can be broken into cinnamic acid and dehydration product of harpagide aglycone.Harpagoside is mainly absorbed by prototype in rat serum after oral administration,and it can be metabolized to cinnamic acid and harpagide in vivo.关键词：harpagoside;stability;cinnamic acid;harpagide;Scrophulariae Radix;artificial gastric juice;intestinal contents
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect and mechanism of the active fraction of Simiao Yongan Tang (AFSM) on vascular remodeling in hypertensive rats induced by nitric oxide synthase inhibitor (L-NAME). Method: Forty-eight Wistar rats were randomly divided into six groups:AFSM high dose group, medium dose group and low dose group (1, 0.5, 0.25 g·kg-1·d-1), captopril group (50 mg·kg-1·d-1), simvastatin positive drug group (10 mg·kg-1·d-1) and model group. L-NAME was administered to the animals in each group. Their body weight, heart weight, blood pressure and heart rate were determined after four weeks of administration. After sacrificing, their aortic thickness changes were measured through HE staining on aortic root slice. Masson staining was used to observe the changes of the coronary artery fibrosis, and the changes of the expression levels of monocyte macrophage antigen-1 (ED-1) and proliferating cell nuclear antigen (PCNA) were observed by immunohistochemistry method. Result: As compared with the model group, only captopril group could alleviate myocardial hypertrophy; captopril group, AFSM high dose and medium dose groups had certain effects in reducing diastolic and systolic blood pressure; there was no effect on heart rate in various treatment groups; captopril group, AFSM high dose and medium dose groups could significantly reduce the aortic wall thickness; captopril group and AFSM high dose group could significantly reduce the formation of peripheral fibrosis of the coronary artery; captopril group and AFSM high dose group could significantly inhibit the expression levels of ED-1 and PCNA in the coronary artery (P<0.05, P<0.01). Conclusion: The AFSM active fractions can reduce hypertension and improve vascular remodeling. The mechanism may be related to inhibiting the anti-inflammatory effect of ED-1 and the anti-proliferative effect of PCNA.关键词：active fraction of Simiao Yongan Tang;hypertensive rat model;vascular remodeling;nitric oxide synthase inhibitor;monocyte macrophage antigen-1;proliferating cell nuclear antigen
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发布时间：2024-01-04 - 摘要：Objective: To screen the effective parts of Descurainiae Semen Lepidii Semen(DSLS) that play a role in anti-heart failure and explore its mechanism. Method: The male sprague dawley (SD) rats were randomly divided into normal group, model group, positive drug group (digoxin group), DSLS low dose, middle dose and high dose groups. The chronic heart failure (CHF) models were established by intraperitoneal injection of doxorubicin for 6 weeks. The drugs were given for 4 weeks by intragastric administration; metabolic cage method was used to collect 6 h urine, and cardiac function changes were measured by ultrasonic echocardiogram. Body weight and heart weight of the rats were measured in each group to calculate the cardiac index after the rats were sacrificed. The levels of brain natriuretic peptide(BNP), cardiactroponin I (cTnI), angiotensin Ⅱ (AngⅡ), and aldosterone (ALD) in blood, as well as the activities of superoxidedismutase (SOD) and methane picarboxylic aldehyde (MDA) contents were measured by ELISA. The activities of glutathione peroxidase (GSH-Px) in myocardial tissues were evaluated by using dithio-bis-nitrobenzoic acid (DTNB) method. Histopathological changes of myocardium were visualized under light microscope. Result: As compared with normal group, the levels of BNP, cTnI, AngⅡ and ALD were significantly higher in model group; left ventricular ejection function (LVEF) and left ventricular fractional shortening rate (LVFS) in model rats were significantly reduced; MDA content was significantly increased; SOD and GSH-Px activities were significantly reduced; urine volume was significantly reduced (P<0.05, P<0.01); with more obvious myocardial pathological injury. As compared with the model group, the effective fractions of DSLS significantly reduced the levels of BNP, cTnI, AngⅡ, and ALD (P<0.05, P<0.01), increased LVEF and LVFS (P<0.05, P<0.01), and reduced myocardial pathological damage in heart failure rats. Moreover, the high-dose and middle-dose DSLS groups significantly reduced content of MDA, and increased activity of SOD (P<0.01), with a good diuretic effect (P<0.05). Meanwhile, middle-dose group could significantly improve the activity of GSH-Px (P<0.05). Conclusion: The water extracts from effective fractions of DSLS could significantly improve the heart failure symptoms in rats. Its mechanism may be associated with improving the unbalance of oxidative stress in vivo and inhibiting the excessive activation of neuroendocrine in CHF rats.关键词：Descurainiae Semen Lepidii Semen;doxorubicin;oxidative stress;heart failure
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发布时间：2024-01-04 -
Therapeutic Effect and Mechanism of Kangfuxin Liquid on Oxazolone-induced Ulcerative Colitis in Rats
摘要：Objective: To study the therapeutic effect of Kangfuxin liquid on oxazolone-induced ulcerative colitis (UC) in rats, and explore its mechanism of action. Method: SD rats were randomly divided into normal group, model group, mesalazine group, and Kangfuxin low dose, middle dose, and high dose groups. In the latter five groups, ulcerative colitis was induced by intrarectal administration of oxazolone. Rats in each group were intrarectally administered with drugs for 7 days, once a day. The disease activity index (DAI) was recorded every day and the rats were sacrificed at the 8th day to evaluate the colonmucosa damage index (CMDI) and the histopathological score (HS). The contents of interleukin (IL)-4 in serum and IL-13 in colonic mucosa were detected by enzyme-linked immunosorbent assay. Result: The DAI score, colon index, CMDI score, HS and IL-13 content in colonic mucosa in model group were significantly higher than those in normal group (P<0.05,P<0.01). The content of IL-4 in serum of model group was significantly lower than that in normal group (P<0.01). As compared with the model group, DAI score, CMDI score, HS and IL-13 content in colonic mucosa were significantly reduced (P<0.05, P<0.01) while the IL-4 content in serum was significantly increased in high dose Kangfuxin group (P< 0.01). Conclusion: Kangfuxin liquid may effectively relieve the symptoms of oxazolone-induced ulcerative colitis.关键词：oxazolone;ulcerative colitis;Kangfuxin liquid;mechanism- 4
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effects and mechanisms of resveratrol on perimenopausal depression model in mice. Method: Clean-grade Kunming mice were randomly divided into the sham group, the model group, the fluovetine group (3 mg·kg-1) and resveratrol groups (10, 20, 40 mg·kg-1), with 10 mice in each group. The mice were gavaged one hour before the daily stress, while the others were gavaged with physiological saline for consecutively 21 days. The perimenopausal depression model in mice was established by Ovariectomy and chronic unpredictable mild stress (CUMS). The general physical signs of the mice were observed, and experience of vaginal epithelial cells was conducted to detect changes in estrus cycle. The depressed behavior of mice was observed by body weight measurement (BWM), open field test (OFT) and foece swimming test (FST). Changes in 5-HT, NE and DA contents in mice brain were detected by ELISA methods. ERα and ERβ protein expressions in hippocampus were detected by IHC method. Result: Compared with the sham group, the mice in OVX group did not show any change in estrous cycle for seven continuous days, indicating the successful modeling. The mice in model group appeared the behavior of depression, their body weight mice were decreased, the scores of horizontal and vertical motion in OFT were decreased, and the behavioral despair time were extended (P<0.01). Nissl's staining showed hippocampus neuron damage, atrophy and loss, and decrease in the number of Nissl bodies. The results of ELISA test showed that the content of 5-HT, NE and DA in brain tissues were decreased (P<0.01); IHC showed that the protein expressions of ERα and ERβ in hippocampus CA3 area were decreased, and IA of ERβ in DG area were decreased. Compared with the model group, after administration with different doses of resveratrol, all of mice in the perimenopausal depression model showed relieve in the abnormalities of behavior, which were characterized by rapid body mass increase, increase in spontaneous activities and exploratory behavior of OFT, reduction in the behavioral despair time of FST (P<0.01), increase in the content of the brain tissue 5-HT, NE and DA (P<0.01), and rise in ERα and ERβ in hippocampus CA3 area and IA of ERβ in DG area (P<0.01). Among each dose group, the medium-dose group was more obvious (P<0.05). Conclusion: Phytoestrogens resveratrol can alleviate perimenopausal depression in mice depressive behavior. Its mechanism is correlated with inhibition in neurons injury, up-regulation in the content of monoamine neurotransmitters 5-HT, NE and DA in brain tissues, and increase in the expression in ERα and ERβin hippocampus CA3 area and IA of ERβin DG area.关键词：resveratrol;ovariectomy;estrogen;Perimenopausal Depression;neurotransmitter;receptor
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of Lagotidis Herba on alpha-naphthyl isothiocyanate (ANIT) induced acute liver injury in mice, and investigate its mechanism of action. Method: The acute liver injury models were induced by intragastric administration of ANIT(100 mg·kg-1). All groups were administered for 7 d, their serum and liver were collected 1 h after the last administration to detect the levels of Alanine transaminase (ALT), aspartate transaminase (AST), total bile acid (TBA), total bilirubin (TBIL) and tumor necrosis factor-α (TNF-α) in serum, the contents of superoxide dismutase (SOD), malonic dialdehyde (MDA) and glutathione peroxidase (GSH-Px) in liver homogenate, and the expression levels of TNF-α mRNA in liver tissues. The pathological changes of the liver tissues were observed by hematoxylin and eosin (HE) staining. Result: As compared with model group, various doses of Lagotidis Herba(1.0, 2.0 and 4.0 g·kg-1) significantly decreased the levels of ALT, AST,TBIL and TNF-α in blood serum and the activity of MDA in liver homogenate, improved the activity of GSH-Px in liver homogenate, 2.0,4.0 g·kg-1 Lagotidis Herba significantly decreased the level of TBA in blood serum, improved the activity of SOD in liver homogenate (P<0.01). As compared with model group, the contents of TBA and SOD of Lagotidis Herba(1.0 g·kg-1) had no statistical difference in 4.0 g·kg-1 Lagotidis Herba group. All doses of Lagotidis Herba significantly decreased the relative expression level of TNF-α mRNA in liver tissue (P<0.01). Furthermore, Lagotidis Herba (2.0, 4.0 g·kg-1) could ameliorate the hepatic pathological changes. Conclusion: Lagotidis Herba has protective effects on acute hepatic injury induced by ANIT in mice.关键词：Lagotidis Herba;liver injury;alpha-naphthyl isothiocyanate (ANIT);Alanine transaminase (ALT);aspartate transaminase (AST);total bile acid (TBA);total bilirubin (TBIL);tumor necrosis factor-α (TNF-α)
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Biejia Jianwan on intestinal flora imbalance and investigate its protection effect on liver in non-alcoholic fatty liver disease (NAFLD) rats model. Method: SD rats were randomly divided into normal group, model group, rosiglitazone (3 mg·kg-1) group and Biejia Jianwan (2.4, 1.2 and 0.6 g·kg-1) groups (n=10 in each group). NAFLD rat models were established by high fat emulsion and carbon tetrachloride olive oil solution. The drugs were given for 4 weeks after successful modeling. The changes in intestinal flora were monitored dynamically. Colon tissues were taken after experiment to detect mRNA expression levels of tight junction protein expression occludin and blocking junction protein (ZO-1) with the method of Real-time PCR. In addition, mucus layer thickness was evaluated by Alcian blue-periodic acid Schiff (AB-PAS) staining. The levels of alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total cholesterol (TC), and low density lipoprotein cholesterol (LDL-C) in blood serum were monitored dynamically. The liver histopathology was observed by means of hematoxylin-eosin (HE) staining. Result: As compared with the normal group, the number of Bacteroidetes, Bacteroides and Fusobacterium was significantly increased (P<0.05) and the number of Lactobacilli was decreased in model group (P<0.05). Besides, the expression level of occludin mRNA was significantly decreased and intestinal permeability was significantly increased (P<0.05) in model group. As compared with the model group, Biejia Jianwan 0.6 g·kg-1 could significantly reduce the number of Bacteroidetes, while increase the number of Lactobacilli and the expression level of occludin mRNA (P<0.05). Biejia Jianwan could also effectively relieve liver damage and improve inflammatory cell infiltration. Conclusion: Biejia Jianwan had protective effects in NAFLD model rats by improving intestinal flora disturbance, effectively reducing intestinal permeability and significantly reducing liver damage.关键词：non-alcoholic fatty liver disease;intestinal flora;intestinal permeability;tight junction protein
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发布时间：2024-01-04 - 摘要：Objective: To explore the mechanism of zedoary turmeric oil on tumor angiogenesis through the research of zedoary turmeric oil effect on vascular endothelial growth factor (VEGF) and chemokine(CXC) protein expression levels in rectal cancer SW1463 cells. Method: Steam distillation was used to extract volatile oil from Rhizoma curcuma, and prepared into different concentrations 80, 120, 160 and 200 mg·L-1. After 24 h treatment on rectal cancer SW1463 cells, their effects on morphology of rectal cancer SW1463 cells were observed by inverted microscope. The protein expression levels of VEGF, CXC, interleukin(IL)-8 and its receptors CXCR2, CXCR3 in rectal cancer SW1463 cells were detected by immunocytochemistry and Western blot. Result: After treatment on rectal cancer SW1463 cells for 24 h by zedoary turmeric oil, the protein expression levels of VEGF, IL-8 and CXCR2 in cells were significantly down-regulated as compared with the normal blank group (P<0.05,P<0.01). The protein expression levels of VEGF, IL-8, CXCR2 could be inhibited in tumor cells by zedoary turmeric oil. However, the protein expression level of CXCR3 in cells was significantly up-regulated as compared with the normal blank group (P<0.05,P<0.01). The protein expression level of CXCR3 can be promoted in tumor cells by zedoary turmeric oil. Conclusion: Different concentrations of zedoary turmeric oil from Guizhou can inhibit rectal cancer SW1463 cells proliferation and alter cellular morphology. Zedoary turmeric oil from Guizhou can inhibit tumor angiogenesis through down-regulation of pro-angiogenesis-associated factors VEGF, IL-8, CXCR2 and up-regulation of angiogenesis-related chemokines CXCR3.关键词：zedoary turmeric oil from Guizhou;rectal cancer SW1463 cells;vascular endothelial growth factor (VEGF);chemokine(CXC);angiogenesis
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of epicatechin on inflammatory cytokines in lipopolysaccharide (LPS)-induced macrophage model and its mechanism. Method: The in vitro inflammation model was established by stimulating RAW 264.7 cells with LPS (1 mg·L-1) for 24 h. The toxic effect of macrophages were detected by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The content of nitricoxide (NO) was assayed by Griess reagent.Enzyme linked immunosorbent assay(ELISA) were used to assay the content of inflammatory mediators, such as tumor necrosis factor-α (TNF-α), interleukin-1(IL-1) and interleukin-6 (IL-6) in cell supernatant. The protein expressions of nitric oxide synthase (iNOS) and the related proteins of mitogen-activated protein kinase (MAPK) pathway were tested by Western blot. Result: The cell viability was not significantly affected by epicatechin at 100 μmol·L-1. Compared with control group, LPS could significantly induce RAW 264.7 cells to secrete inflammatory mediators, like NO, TNF-α, IL-1 and IL-6(P<0.01). Compared with model group, 25-100 μmol·L-1of epicatechin in LPS-induced RAW 264.7 cells greatly inhibited the release of inflammatory mediators, such as NO, TNF-α, IL-1 and IL-6 (P<0.05, P<0.01), and inhibit the expressions of iNOS, and the protein expression of p38MAPK, ERK1/2 and JNK in a dose dependent manner. Conclusion: Epicatechin can inhibit LPS-induced inflammatory response in RAW 264.7 cells, and its anti-inflammatory effect may be related to reduction of inflammatory cytokines, like NO, TNF-α, IL-1 and IL-6, inhibition of the gene expression of iNOS and phosphorylation of p38MAPK, ERK1/2 and JNK.关键词：epicatechin;lipopolysaccharide;tumor necrosis factor-α(TNF-α);interleukin-1(IL-1);interleukin-6(IL-6);nitric oxide synthase (iNOS);mitogen-activated protein kinase (MAPK)
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Ypsilandra thibetica 16 (YB16) extracted from the Ypsilandra thibetica on proliferation and apoptosis of human prostate cancer cells PC-3, and explore its mechanism. Method: PC-3 cells were cultured in vitro and treated with different concentrations of YB16 (0.125~16 μmol·L-1). The effects of YB16 on PC-3 cytotoxicity were detected by (4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, and the cell morphology was observed by phase contrast microscope. Acridine orange (AO) staining and flow cytometry were used to detect the apoptosis of PC-3 cells induced by YB16.Reverse-transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of B-cell lymphoma 2 gene (Bcl-2), B-cell lymphoma 2 gene-xl (Bcl-xl), Bcl-2 associated X protein (Bax), and cysteinyl aspartate specific proteinase-3(Caspase-3)of PC-3 cells, and Western blot was used to detect the protein expression levels of Bcl-2, Bcl-xl, Bax and cleaved-Caspase-3. Result: YB16 could significantly inhibit the growth of PC-3 cells in a dose-dependent manner (P<0.05). YB16 could promote PC-3 cells apoptosis, and PC-3 cells had obvious characteristic apoptosis changes in the observation under phase contrast microscope and AO staining. YB16 could reduce the mRNA and protein expression levels of Bcl-2, Bcl-xl, but increase the mRNA and protein expression levels of Bax, Caspase-3 (P<0.05). Conclusion: YB16 can inhibit PC-3 cells proliferation, and induce apoptosis obviously. YB16 has a potential antitumor value, and its mechanism may be associated with promoting Caspase-3 expression.关键词：steroidal saponin YB16;prostate cancer;PC-3 cells;apoptosis;B-cell lymphoma 2 gene (Bcl-2);B-cell lymphoma 2 gene-xl (Bcl-xl);Bcl-2 associated X protein (Bax);cysteinyl aspartate specific proteinase-3 (Caspase-3)
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the in vitro anti-HIV-1 activities and mechanism of extracts from 6 traditional Tibetan medicines:Corydalis conspersa, Fallopia aubertii, Anemone vitifolia, Clematis urophylla, Saxifraga melanocentra and C. hexapetala. Method: Cell counting kit(CCK-8) cell cytotoxicity test and HIV-1 single cycle infection experiments were used to detect the in vitro anti-HIV-1 effect and cytotoxicity of extracts. The surface plasma resonance(SPR) technology, protease, integrase and reverse transcriptase inhibition assay were used to identify the potential targets of these extracts. Result: The extracts from F. aubertii, A. vitifolia and S. melanocentra showed dose-dependent anti-HIV-1 effect with little cytotoxicity. Their half maximal inhibitory concentrations (IC50) were (6.47±0.78), (11.97±1.09), (11.7±0.79) mg·L-1, respectively. All the above three extracts inhibited integrase activity. Conclusion: F. aubertii, A. vitifolia and S. melanocentra extracts potently inhibited HIV-1 virus in vitro, possibly through down-regulating integrase activity.关键词：Tibetan medicine;HIV-1 virus;protease;integrase;reverse transcriptase
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发布时间：2024-01-04 - 摘要：Objective: To study the accurate curative effect of traditional Chinese medicines (TCM) compound Jinfukang in treating lung cancer, and optimize the prescription composition to inhibit lung cancer cell proliferation, so as to provide a new approach for the optimization of TCM compound prescription. Method: Screening test design (Plackett-Burman) and random forest algorithm were applied in establishing the prediction model based on design prescription set. With 61 composition design matrixes as the input, and natural logarithm of their corresponding IC50 as the output, random forest regression model was establish. The optimization model parameter mtry was verified by 5-time and 10-fold interaction. Finally, a grid search algorithm was applied to get the optimal group, and verify the lung cancer cell proliferation experiment. With the IC50 obtained from different formula of Jinfukang on A549 lung adenocarcinoma cancer cells as an index, the composition of Jinfukang were optimize to inhibit lung cancer cell proliferation. Result: Through random forest model combined with grid search algorithm, we got the optimal compatible herbs, namely Astragali Radix, Ophiopogonis Radix, Paridis Rhizoma, Ligustri Lucidi Fructus and Gynostemmatis Pentaphylli Herba seu Radix. Through the experiment, optimized Jinfukang showed a better effect in cell inhibiting and proliferation than original Jinfukang in the aspects of inhibition of lung cancer cell proliferation. Conclusion: The random forest model combined with the grid search algorithm complexity can provide methodology reference for the optimization of composition of complex TCM compound formula.关键词：random forest;Jinfukang;cell proliferation;composition optimization
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发布时间：2024-01-04 - 摘要：Objective: To assess the antifungal effect of Dryopteris fragrans effective parts ointment(DF ointment) in the Malassezia furfur-infected skin model in guinea pigs. Method: Guinea pigs infected with M. furfur for 7 days were divided into 5 groups on the 8th day according to skin lesion scores:namely model group, ketoconazole group (1.33 mg·g-1), high-dose, moderate-dose and low-dose DF ointment groups (containing 2, 1, 0.5 g·g-1 of crude herb). The groups were treated with corresponding drugs for 10 days, qd. The efficacy of DF ointment was evaluated through observation of changes in skin lesion score, CFU number, negative rate and PAS staining. Result: High-dose, moderate-dose and low-dose DF ointment groups showed lower skin lesions(P<0.05) and CFU number (P<0.05) than model group, and higher negative rate (P<0.05, P<0.01). PAS staining revealed fewer spores in High-dose and moderate-dose DF ointment groups than model group. Conclusion: DF ointment has a better therapeutic effect on M. furfur-infected skin model in guinea pigs.关键词：Dryopteris fragrans effective parts ointment;in vivo antifungal activity;malassezia furfur;guinea pig model
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Yangyin Shugan capsule on ovarian function in patients with primary ovarian insufficiency (POI), and explore its mechanism of action. Method: In this randomized, double-blind trial, 60 patients with POI were recruited and randomly divided into treatment group (Yangyin Shugan capsule, 4 capsules/time, po, tid) and control group (placebo therapy, 4 capsules/time, po, tid), 30 cases in each group. All patients were treated for 12 weeks. After treatment, the sex hormone levels (FSH, LH), antral follicle count and ovarian blood were compared between the two groups. Result: As compared with control group, the levels of basal follicle-stimulating hormone (bFSH) and basal luteinizing hormone (bLH) were significantly lower in treatment group, so it was believed that Yangyin Shugan capsule had better effect on improving bFSH, bLH levels (P<0.05); the antral follicle count and ovarian blood flow were significantly improved in treatment group (P<0.05). Conclusion: Yangyin Shugan capsule can effectively regulate the basal sex hormone levels, increase the basal antral follicle counts and improve blood flow to improve the ovarian function in patients with POI, with obvious clinical efficacy.关键词：Yangyin Shugan capsule;primary ovarian insufficiency;influence mechanism;basal follicle-stimulating hormone (bFSH);basal luteinizing hormone(bLH)
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Bushen Huoxue Tongluo decoction in treatment of glucocorticoid-induced osteoporosis (GIOP), and investigate its mechanism of action. Method: The 120 cases of GIOP patients were randomly divided into observation group and control group, 60 cases in each group. Patients in observation group took prednisone hormone and Bushen Huoxue decoction, and patients in control group received glucocorticoid, calcitriol capsules and calcium carbonate D3 tablets for 6 months. Before and after treatment, the two groups were evaluated by traditional Chinese medicine (TCM) syndrome scores, curative effects, levels of blood calcium, blood phosphorus, urinary calcium, urinary phosphorus, serum alkaline phosphatase (ALP), serum bone gla protein (BGP) and blood parathyroid hormone (PTH), bone mineral density (BMD) value and adverse reactions. Result: The markedly effective rate was 53.3% in observation group, higher than 30.0% in control group (P<0.05), and there was no difference in total effective rate between two groups. As compared with before treatment, total TCM syndrome scores were decreased in two groups; levels of blood calcium and phosphorus were increased, and BMD values were increased, with significant difference (P<0.05). As compared with control group, soreness and weakness of waist and knees, low back pain symptoms, leg pain, walking hardship symptom and total scores were decreased significantly after treatment in observation group (P<0.05), levels of serum PTH were decreased and BMD values were increased (P<0.05). The incidence of adverse effects was 0 in observation group, and 10% in control group (P<0.05). Conclusion: Bushen Huoxue Tongluo decoction has satisfactory efficacy for GIOP, which can reduce the levels of hormones, side effects and the incidence of fractures, with high safety.关键词：Bushen Huoxue Tongluo decoction;glucocorticoid;osteoporosis;alkaline phosphatase (ALP);serum bone gla protein (BGP);blood parathyroid hormone (PTH);bone mineral density (BMD)
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of modified Linggan Wuwei Jiangxintang combined with seretide in treating chronic persistent bronchial asthma and investigate its effects on levels of interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin-13 (IL-13), and interleukin-17 (IL-17). Method: One hundred and forty patients were randomly divided into control group (69 cases) and observation group (71 cases) by random number table. Patients in control group received treatment of oxygen therapy, reducing phlegm, spasmolysis and infection control, and they took salmeterol xinafoate and fluticasone propionate powder for inhalation (seretide) for every morning and night, 50/250 μg/time; for severe patients, compound Methoxyphenamine capsules were added for 7 days, 2 capsules/time, 3 times/day. Based on the treatment in control group, patients in observation group added modified Linggan Wuwei Jiangxintang, 1 dose/day. The treatment course was 12 weeks for both groups. Before and after treatment, lung function was detected; forced expiratory volume in one second account for predicted value (FEV1), and maximal expiratory flow account for normal reference value (PEF) were recorded, score of the asthma was graded by asthma control test (ACT), and score of phlegm wheezing was also graded. Levels of IL-4, IL-10, IL-13 and IL-17 were also detected before and after treatment. Result: The total effective rate was 95.77% in observation group, higher than 85.51% in control group (P<0.05). The total effective rate for TCM symptoms was 94.37% in observation group, higher than 79.71% in control group (P<0.01). In Ridit analysis, the controlling situation for asthma in observation group was superior to that in control group (P<0.05). After treatment, the levels of FEV1 and PEF were increased in both groups (P<0.01), and these levels in observation group were higher than those in control group (P<0.01). The levels of IL-4, IL-13 and IL-17 in observation group were lower than those in control group, and level of IL-10 was higher than that in control group after treatment (P<0.01). Conclusion: Modified Linggan Wuwei Jiangxintang combined with seretide can control asthmatic attack, relieve clinical symptoms, ameliorate lung function, and improve clinical efficacy in treatment of chronic persistent bronchial asthma, and the mechanism of action may be associated with regulating levels of IL-4,IL-10, IL-13 and IL-17.关键词：bronchial asthma;chronic persistent;phlegm wheezing symptoms;modified Linggan Wuwei Jiangxintang;interleukin (IL)-4;IL-10;IL-13;IL-17
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发布时间：2024-01-04 - 摘要：The toxically study of Chinese herbal compound is one of key problems in traditional Chinese medicine (TCM).By reviewing toxicity of Chinese herb in TCM theory and drug matching theory in Chinese herbal compound,this paper considers that toxicity of Chinese herbal compound is adverse side of its efficacy and pharmacological function under the guide of Chinese medicine theory,and it also changes along with physical states.Thus the relation of mutual dependence and transformation always exists between efficacy and toxicity of Chinese herbal compound.By modern research of efficacy of Chinese herbal compound,the efficacy and toxicity theory of Chinese herbal compound will be better elucidation,which can provide scientific evidence for the rational and safe use of Chinese herbal compound in clinic.关键词：traditional Chinese medicine;compound;efficacy;toxicity;biomarkers;research approaches;corresponding of prescription and symptom
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发布时间：2024-01-04 -
Progress and Cause Analysis on Causes of Adverse Reactions of Polygoni Multiflori Radix Preparations
摘要：Progress and cause analysis of adverse reactions of Polygoni Multiflori Radix preparations were studied to conduct follow-up researches from the perspective of the effect of preparations in toxic expression and provide research ideas. Relevant literatures in recent years were consulted to study Polygoni Multiflori Radix preparations for their adverse reactions and toxicity mechanism and other aspects, in order to summarize and analyze the causes. In terms of components, Polygoni Multiflori Radix mainly containing stilbene, lecithin, anthraquinones, tannins, trace elements and other ingredients, with stilbene glucoside and anthraquinone class as its main active ingredients, which indicated the possible material basis of Polygoni Multiflori Radix toxicity; with respect to processing, processing methods had effects on the chemical composition of Polygoni Multiflori Radix, as evidenced by significant differences in Polygoni Multiflori Radix blood profiles before and after processing, which caused different liver substance metabolic mechanisms in rats. By dosage and usage, long-term intake of high-dose Polygoni Multiflori Radix can cause reversible liver damage, which was mainly reflected in liver function abnormalities. In terms of compound compatibility, it was still uncertain whether different combinations would result in Polygoni Multiflori Radix adverse reactions. Clinical adverse reactions of Polygoni Multiflori Radix preparations suggest toxic and side effects that cannot be ignored on liver. Modern preparation process may be one of the causes for the adverse reactions, but its regularity characteristics causing liver toxicity, mechanism of action and material basis were still unclear yet. In addition to the chemical constituents in Polygoni Multiflori Radix and the attenuating mechanism of processing, efforts shall be made to actively explore the impact of the effect of pharmaceutical process of Polygoni Multiflori Radix on its hepatotoxicity. In addition to strengthening safety evaluation of Polygoni Multiflori Radix preparations, efforts shall be made to actively study the effect of pharmaceutical processing, compound compatibility and drug interactions on hepatotoxic effects and explore rational clinical drug use patterns, in order to minimize the adverse reactions caused by taking of Polygoni Multiflori Radix preparations.关键词：Polygoni Multiflori Radix preparation;adverse reactions;cause analysis- 2
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发布时间：2024-01-04 - 摘要：Cancer has become a threat to human health, one of the major devastating diseases. Due to the lack of effective treatments, the number of deaths from cancer is still showing a trend of increasing year by year. Based on the overall macro-dialectical thinking mode, and with the unique effect and low side effect, traditional Chinese medicine(TCM) has attracted the attention of cancer researchers and clinicians from around the world. Bitter-cold herbs have significant anti-tumor effects. In this paper, we reviewed the research progress of these herbs and their active constituents by referring to the relevant literatures published in the last decade at home and abroad. According to the function of bitter-cold herbs and their natural active ingredients, this review was divided into nine parts:induction of tumor cell apoptosis, induction of cancer cell differentiation, anti-cancer invasion and anti-metastasis effect, inhibition of tumor angiogenesis, improvement of immune function, reversal of multi-drug resistance, anti-information transmission, inhibition of telomerase activity and cell cycle arrest. In addition, the current situation and existing problems were reviewed in order to screen the anti-tumor Chinese medicine and their active components, thus discovering new drugs with high efficiency and low toxicity from TCM, and benefiting further clinical application.关键词：anti-tumour;bitter and cold Chinese herb;mechanism;active ingredients;research progress
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发布时间：2024-01-04 - 摘要：Snakes are not only precious wildlife resources, but also an important source of medicinal snakes, and it has a good effect in the treatment of rheumatic arthralgia syndrome. In recent years, driven by economic interests, coupled with more species and inconspicuous morphological differences, there are a large number of adulterants. Paper focuses on the identification methods of medicinal snakes and its adulterants over the last decade, and a comprehensive overview of various identification methods researches progress in this herb were displayed, including the character identification, microscopic identification, physical and chemical identification and molecular identification, and the applicable objects, advantages and disadvantages of each method of identification are described and discussed.It is found that medicinal snakes in the field of fast check, identification of Traditional Chinese Medicine Patent Prescription are still faced with some difficulties, and it showed that traditional and modern methods should be combined,authenticity of identificationand pros and cons of identification should be combined when objective assessment of medicinal snakes.关键词：medicinal snakes;adulterants;identification methods;molecular identification
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发布时间：2024-01-04 - 摘要：This paper aims to reveal the existing problems of uniformity of Chinese materia medica solid preparation,discuss the causes and impact of problems,and provide the reference for consistency of the drug production.Through searching the literature reports about uniformity of solid preparations of Chinese medicine in the recent ten years,the problem of uniformity of Chinese materia medica solid preparation from the three aspects in physics,chemistry and biological effects were discussed,the causes of poor uniformity of solid preparation were discussed,the effect of effectiveness,safety and controllability of solid preparation that caused by ununiformity of solid were analysed.There were difference among solid preparations in physics,chemistry and biological effects,which mainly caused by the difference of production technics.Drug efficacy and safety were affeced by uniformity problems of solid preparation,and insiders should pay more attention to this problem.关键词：Chinese materia medica;solid preparation;uniformity;content difference;technics
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发布时间：2024-01-04
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