最新刊期
卷 23 ， 期 3 ， 2017
- 摘要：Objective: To research on rationality of peeling in the habitat processing of Smilacis Glabrae Rhizoma. Method: The fresh Smilacis Glabrae Rhizoma was divided into three groups of peeling group,non-peeling group and peel group,then they were processed by drying at 35, 55,80℃,in the shade and the sun,the content of total flavonoids was measured by UV with rutin as reference substance,the contents of taxifolin,astilbin and resveratrol ware determined by HPLC,the contents of five kinds of harmful heavy metals (Cu,Pb,Cd, Hg and As) were measured by ICP-MS,in order to compare the differences between them. Result: The contents of total flavonoids,taxifolin,astilbin and resveratrol in peel group were higher than those in peeling group and non-peeling group,the above ingredients in non-peeling group were higher than those in peeling group.The contents of Cu and As in peel group were higher than those in peeling group and non-peeling group,the content of Pb in peel group and non-peeling group were higher than that in peeling group,all elements of the three groups were lower than the limited values in 2015 edition of Chinese Pharmacopoeia. Conclusion: The effective components and the heavy metals of Smilacis Glabrae Rhizoma are concentrated in the epidermis,for medicinal purposes,you do not need to peel;for edible,in order to avoid heavy metal accumulation,you can choose to peel.关键词：Smilacis Glabrae Rhizoma;habitat processing;peeling;astilbin;resveratrol;taxifolin;heavy metals
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发布时间：2024-01-04 - 摘要：Objective: To observe the compatible stability of Danhong injection combined with citicoline sodium injection (DHI+CSI) in two commonly used solvents (0.9% sodium chloride injection and 5% glucose injection). Method: The characters,pH values and insoluble particles of DHI+CSI in two solvents were observed and determined.The content changes of main compounds including danshensu,protocatechuic aldehyde,caffeic acid,p-coumaric acid,rosmarinic acid,salvianolic acid B and citicoline sodium in DHI and CSI were determined by UPLC. Result: The characters of DHI+CSI in the two commonly used solvents were brown,clarify and transparent liquid;pH values within 4 h were relatively stable;the numbers of insoluble particles were in the range of standards in 2015 edition of Chinese Pharmacopoeia.When the solvent was 0.9% sodium chloride injection,the content of caffeic acid reduced more than 50%;there were no significant changes of the contents of other compounds,and the contents of them were relatively stable within 4 h.When the solvent was 5% glucose injection,there were no significant changes of the contents of seven compounds in DHI and CSI,and the contents of them were relatively stable within 4 h. Conclusion: DHI and CSI can be combined in 0.9% sodium chloride injection and 5% glucose injection,and 5% glucose injection is preferred as the solvent.关键词：Danhong injection;citicoline sodium injection;compatibility;insoluble particles;caffeic acid;salvianolic acid B
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发布时间：2024-01-04 - 摘要：Objective: To compare effect of stir-frying with sized salt and baked with salt solution on contents of β-hydroxyecdysone,25R-inokosterone and 25S-inokosterone in Achyranthis Bidentatae Radix. Method: HPLC was adopted to determine contents of β-hydroxyecdysone,25R-inokosterone and 25S-inokosterone with mobile phase of acetonitrile-water-formic acid (16:84:0.1) and detection wavelength of 250 nm.Raw products of Achyranthis Bidentatae Radix was used as reference to examine effect of these two salting technologies on contents of index ingredients. Result: Contents of index ingredients in samples baked with salt solution were the highest when 1 g of salt was included in 100 g of Achyranthis Bidentatae Radix,and being fried for 15 mins at 150-180℃;those in samples stir-fried with sized salt were the highest when 2 g of salt was included in 100 g of Achyranthis Bidentatae Radix,and being fried for 4 mins at 150-180℃.Contents of β-hydroxyecdysone,25R-inokosterone and 25S-inokosterone in No. 3 samples were the highest among samples baked with salt solution,which were 0.083%,0.015 7%and 0.010%; the highest contents of these three ingredients of samples stir-fried with sized salt were 0.100%,0.019 1%,0.012 3%,respectively. Conclusion: Contents of three kinds of ketosteroid ingredients in samples stir-fried with sized salt are more than those in raw products and samples baked with salt solution,different salting technologies both have effect on effective ingredients in Achyranthis Bidentatae Radix.关键词：Achyranthis Bidentatae Radix;baked with salt solution;stir-frying with sized salt;β-hydroxyecdysone;25R-inokosterone;25S-inokosterone;processing technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize fermentation process of Massa Medicata Fermentata by investigating its different fermentation way. Method: Using the amylase and protease activity combining with HPLC specific chromatogram as the evaluating indicators,unfermentation,constant temperature and humidity fermentation,natural room temperature fermentation of Massa Medicata Fermentata were compared to optimize the best fermentation way,then do comparative study with commercially available Massa Medicata Fermentata(Z raw product).Taking the activity of amylase and protease as indicators,single factor tests were carried out on the fermentation humidity and additive amount of herbal medicine juice to optimize the best process. Result: The best fermentation way was constant temperature and humidity fermentation,its activities of amylase and protease were 14.768 mg·min-1·g-1 and 79.467 μg·min-1·g-1,its chemical composition was similar to natural fermentation and Z raw product.Compared with reference chromatogram,the similarity values were all higher than 0.9 except the unfermentation group.The best fermentation humidity was 80% and the best additive amount of herbal medicine juice was 65 mL for 100 g of bran-flour. Conclusion: The digestive enzyme activity of Massa Medicata Fermentata fermented with constant temperature and humidity is high,its fermentation speed is faster than the natural room temperature fermentation,its fermentation process is controllable and feasible,which can provide a scientific reference for formulating quality standard of Massa Medicata Fermentata in future.关键词：Massa Medicata Fermentata;fermentation process;enzyme activity;specific chromatogram;amylase;protease
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of couplet medicines of Sophorae Flavescentis Radix-Glycyrrhizae Radix et Rhizoma and to quickly identify the chemical constituents in its extract. Method: Taking transfer rates of matrine,oxymatrine and glycyrrhizic acid as comprehensive evaluation index,based on single factor tests,optimum extraction conditions of couplet medicines extract were selected by orthogonal test.The chemical constituents of couplet medicines extract were identified on-line by UPLC-Q-TOF/MS. Result: Optimum reflux extraction process was as follows:soaked for 1 h and extracted twice with 8 times the amount of 60% ethanol,2 h for each time.Total transfer rate of matrine and oxymatrine was 93.92%,the extraction yield of glycyrrhizic acid was 99.23%.Furthermore,a total of 49 constituents were identified in couplet medicines extract,28 constituents were from Sophorae Flavescentis Radix and 21 constituents were from Glycyrrhizae Radix et Rhizoma. Conclusion: The mixed extract has a certain auxo-action for extraction of matrine,oxymatrine and glycyrrhizic acid and the optimum extraction process is stable and feasible.Identification of chemical constituents in extract will be useful for elucidation of its active constituents and compatible mechanism.关键词：Sophorae Flavescentis Radix-Glycyrrhizae Radix et Rhizoma;couplet medicines;extraction technology;matrine;oxymatrine;glycyrrhizic acid
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of endophytic fungal elicitors, which were separated from different parts of Rehmannia glutinosa, on growth and secondary metabolites contents ,in R. glutinosa tissue culture, and make a preliminary study on growth-promoting role of endophytic fungal elicitor. Method: Alternaria alternata, and Penicillium axalicum were used to prepare two different types of elicitors, filtered fermentation liquor and mycelium degradation solution. Then their effects on plant height, fresh weight, chlorophyll content, catalpol and acteoside contents in R. glutinosa tissue culture were investigated. The data analysis was done by using SPSS. The contents of catalpol and acteoside were detected by HPLC. Result: Different fungal elicitors showed significant differences in growth-promoting role. The fungal elicitors of P. axalicum showed a better promoting role than others, and its fermentation filtrate showed the best effect. In this condition, the plant height, fresh weight, root length, chlorophyll content, catalpol and acteoside contents of R. glutinosa tissue culture increased respectively by 27.80%, 29.49%, 22.80%, 15.05%, 35.98% and 68.18%. Conclusion: Endophytic fungal elicitor of R. glutinosa can promote the growth of tissue culture, and improve the contents of its secondary metabolites. Further studies are needed for endophytic fungal applications in R. glutinosa.关键词：endophytic fungal elicitors;Rehmannia glutinosa;secondary metabolites;promoting role
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发布时间：2024-01-04 - 摘要：Objective: To provide guidance for determining the optimal harvesting time,and controling the quality of Polygonati Odorati Rhizoma by measuring the contents of main active ingredients in Polygonati Odorati Rhizoma from different growing ages and harvesting time,and exploring their dynamic variation rule. Method: UV-visible spectrophotometry was used to measure the contents of polysaccharides,flavonoids,saponins,alcohol-soluble extracts in Polygonati Odorati Rhizoma from different growing ages and harvesting time,multiple analysis of variance was used to analyze the experimental results. Result: The variation rule of the contents of main active ingredients in Polygonati Odorati Rhizoma in harvesting time was slow at the early growth, faster at medium-term,slow down at the late growth,and the contents of every ingredients changed to be steady after the end of September,the contents of polysaccharides, saponins,flavonoids,alcohol-soluble extracts in Polygonati Odorati Rhizoma were 7.69%,0.453%,0.141%,57.48% at this stage.The contents of polysaccharides,saponins,flavonoids,alcohol-soluble extracts in Polygonati Odorati Rhizoma didn't sustained increase rapidly with the increase of growing years,and reached the peak after 3 years growth,the contents were 7.67%,0.436%,0.141%,55.59%. Conclusion: The main active ingredients were constantly accumulated with plant growth,but its content did not always maintain a high growth state. The optimal harvest year was 3-year-old,the optimal harvest time was the end of September,the study of dynamic variation rule could provide certain reference basis for improving the medical quality of Polygonati Odorati Rhizoma.关键词：Polygonati Odorati Rhizoma;active ingredients;polysaccharides;Saponins;flavonoids;dynamic variation;harvesting time
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC method for the determination of kaempferol in Kaempferiae Rhizoma, analyze the contents of kaempferol in Kaempferiae Rhizoma from different origins, and provide reference for the evaluation of the medicinal quality. Method: ODS2 C18 (4.6 mm×150 mm, 5 μm) was ised as chromatographic column, with methanol-0.4% phosphate (50:50) as the mobile phase; the flow rate was 1 mL·min-1, column temperature was room temperature; the detection wavelength was set at 367 nm; and the injection volume was 10 μL. Result: Kaempferol showed good linear relationship in the concentration range of 0.015 8-1.58 μg; the recovery rate was 97.8%, RSD of 1.6%. Conclusion: There was great difference in content of kaempferol in Kaempferiae Rhizoma from different origins. The established method is simple, accurate, repeatable, and can be used for quality control of Kaempferiae Rhizoma.关键词：HPLC;Kaempferiae Rhizoma;kaempferol
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发布时间：2024-01-04 - 摘要：Objective: To extract 24 trace elements from Moutan Corter such as Fe, Mn, Cu, Zn, Al, Pb, As and Cd by using decocting method. Method: Soluble state and suspension state in the extract were separated by 0.45 μm microporous filtering film. Soluble state was eluted from water and methanol by macrororous adsorption resin (LSA-10) in order to determine the inorganic state and organic state of the trace elements. In simulated human gastrointestinal solution, n-octylalcohol-water allocation system was used to separate water-soluble state and alcohol-soluble state for the trace elements. The total contents of the trace elements and various forms were detceted by inductively coupled plasma mass spectrometry (ICP-MS). Result: The recovery rate of this method was 74.7%-118.2%for all the elements, and precision RSD was 1.3%-6.9%. The extractive rate of the elements was 2.0%-82.9%, and the ratio of water-soluble state was 73.6%-99.5%. More than 80%of the trace elements in soluble state were in the form of inorganic state and water-soluble state. Conclusion: The morphological analysis and dissolution characteristics provide theoretical basis for clinical application of Moutan Corter.
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components of Glycyrrhizae Radix et Rhizoma in Pinelliae Rhizoma Praeparatum (PRP) by using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS/MS). Method: The aqueous extracts of PRP were separated on Waters Acquity BEH C18 column (2.1 mm×100 mm,1.7 μm) with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. The flow rate was 0.25 mL·min-1 and the column temperature was 35℃. Mass spectrometric data were obtained under electrospray ionization (ESI) negative mode and the constituents were identified according to the exact relative molecular weight and fragment ions reported in the references. Result: Fifteen constituents were identified from Glycyrrhizae Radix et Rhizoma in PRP, including 6 triterpenoid saponins and 9 flavonoids. Conclusion: Systematic research of the fifteen chemical constituents of Glycyrrhizae Radix et Rhizoma in PRP was performed firstly in this study, which could provide the basis for clarifying the pharmacodynamic material basis and rational clinical application of PRP.关键词：UPLC-Q-TOF-MS/MS;Pinelliae Rhizoma Praeparatum;Glycyrrhizae Radix et Rhizoma;chemical components
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发布时间：2024-01-04 - 摘要：Objective: To isolate and identify the contents of scutebarbatine A from Scutellariae Barbatae Herba. Method: Scutebarbatine A was isolated from Scutellariae Barbatae Herba by chromatographic separation method and its structure was determined by spectroscopic method. Agilent-Eclipse XDB-C18 column (4.6 mm×250 mm, 5 mm) was used with methanol-water (60:40) as the mobile phase at a flow rate of 1 mL·min-1. The detection wavelength was set at 260 nm and the column temperature was 40℃. This method was used to investigate the differences of scute barbatine A contents in Scutellariae Barbatae Herba of different origins. Result: The calibration curves of scutebarbatine A showed good linearity over the ranges of 0.030 34-1.213 6 μg (r=0.999 9). The repeatability RSD was 1.8%; precision RSD was 0.5%; and the test sample solution was stable within 16 h, with an average recovery rate of 100.2% (RSD 3.6%). Conclusion: The quantitative determination method to isolate and identify scutebarbatine A from scutellariae Barbatae Herba is controllable, scientific, and suitable for the quality control of Scutellariae Barbatae Herba.关键词：Scutellariae Barbatae Herba;scutebarbatine A;structure identification;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba. Method: The compounds were isolated and purified by recrystallization, silica gel, Sephadex LH-20, and semi-preparative HPLC. Their structures were identified on the basis of spectroscopic methods combined with physicochemical characteristics. Result: From the systemic analysis on the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba (macroporous resin 50%ethanol, and 100%ethanol), thirteen compounds were isolated from the anti-inflammatory fractions and were identified as 4'-hydroxyacetophenone (1), 2,5-dihydroxycinnamic acid ethyl ester (2), quercetin (3), luteolin (4), 7S,8R-dihydrodehydroconiferyl alcohol (5), prudomestin (6), 7,4'-dimethoxykaempferol (7), quercetin-7-O-α-L-rhamnoside (8), 9S,12S,13S-trihydroxy-10E-octadecenoate (9), isorhmnetin-4'-O-β-D-glucoside (10), kaempferol-3-O-β-D-glucoside (11), stearic acid (12) and β-daucosterol (13) respectively. Conclusion: All the above 13 compounds were obtained from the formula granules of Artemisiae Scopariae Herba for the first time; compounds 9-11 were obtained from genus Artemisia for the first time; and compounds 2 and 5 were isolated from Artemisia scoparia for the first time.
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发布时间：2024-01-04 - 摘要：Objective: To establish the near-infrared spectroscopy(NIRS) quantitative model to determine the contents of dehydrocorydaline, tetrahydropalmatine and corydaline in Corydalis Rhizoma rapidly. Method: High performance liquid chromatography (HPLC) was used to determine the authentic contents of dehydrocorydaline, tetrahydropalmatine and corydaline in Corydalis Rhizoma.The spectrum was collected through near infrared diffuse reflection spectroscopy. Then the authentic contents were corresponded to the near infrared spectrum one by one. The correction model was established by partial least squares(PLS) and optimized by multiple scattering correction (MSC) & first derivative. Result: Correlation coefficient (R) was 0.987, 0.998, and 0.997 respectively fordehydrocorydaline, tetrahydropalmatine, and corydalinecorrection models respectively. The validation set indicated that the correlation coefficient between predicted content and authentic content of dehydrocorydaline, tetrahydropalmatine and corydaline was 0.943, 0.978 and 0.991 respectively, and their relative deviation was -14.75%-18.08%, -4.73%-9.23% and -10.99%-9.70% respectively. Conclusion: The established NIRS quantitative model had high prediction accuracy, and could be used to determine the contents of three alkaloids in Corydalis Rhizoma rapidly and synchronously.关键词：Corydalis Rhizoma;near-infrared spectrometry (NIRS);dehydrocorydaline;tetrahydropalmatine;corydaline
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发布时间：2024-01-04 - 摘要：Objective: To screen the diuretic active fractions from rattan of Akebia trifoliate and study its chemical constituents. Method: The 95%ethanol extract of rattan of A. trifoliate, which were extracted with petroleum ether, ethyl acetate, butanol and water dissolving fractions, were investigated through metabolic cages experiments. The compounds of active fractions were isolated and purified by silica gel column chromatography and other methods, and their structures were identified by NMR and MS combined with literature data. Result: The butanol extract fraction can significantly increase the urine volume of rats(P<0.01), but the diuretic effect of other fractions was not obvious. Nine compounds were isolated from this diuresis fraction, and their structures were identified as D-galactose (1), syringicacid-4-O-α-L-rhamnopyranoside (2), hederagenin(3), inositol(4), mannitol (5), vanillic acid-β-glucoside (6), glucosyringic acid (7), hederagenin-3-O-α-L-arabinopyranoside (8) and β-sitosterol-3-O-β-D-glycoside (9). Conclusion: The butanol extract fraction had significant diuretic function, and glycosides were the main chemical composition of this fraction.关键词：rattan of Akebia trifoliata;diuretic effect;chemical constituent;isolation and identification
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous contents determination of 4 flavonoids including quercetin, kaempferide, pinocembrin, and galangin as well as alkaloids piperine in Uighur medicine compound Xiaoaifei by using HPLC. Method: Separation was performed on Hypersil BDS C18(4.6 mm×250 mm, 5 μm) column with acetonitrile (A)-1% phosphoric acid (B)-methanol (C) (0-12 min, 28%A:52%B:20%C; 12-13 min, 28%-30%A, 52%-50%B, 20%C; 13-45 min, 30%A:50%B:20%C) as the mobile phase for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 280 nm; the column temperature was 30℃, and the sample size was 20 μL. Result: Y=357 314X-88 066 (r=0.999 9), Y=442 853X-74 204 (r=0.999 9), Y=647 926X-327 436 (r=0.999 9), Y=471 672X-256 408 (r=0.999 9), and Y=290 000X-391 906 (r=0.999 9), respectively in the calibration curves of quercetin, kaempferide, pinocembrin, galangin and piperine, showing good linear relationship. The mean recoveries (n=9) of them were 98.4% (RSD 1.6%), 98.9% (RSD 1.7%), 101.3% (RSD 2.2%), 98.6% (RSD 2.1%) and 98.6% (RSD 1.8%), respectively. Conclusion: The developed method is simple, accurate, reliable, and helpful to control the quality of Uighur medicine compound Xiaoaifei.关键词：Uighur medicine compound Xiaoaifei;quercetin;kaempferide;pinocembrin;galangin;piperine
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for extraction, isolation and determination of ellagic acid and 3,3-di-O-methylellagic acid from Macrosolen cochinchinensis. Method: The ellagic acid and 3,3-di-O-methylellagic acid were isolated by solvent extraction, silica gel column chromatography and recrystallization techniques. Then HPLC content determination method was established:Eclipse XDB-C18 chromatographic column (4.6 mm×250 mm, 5 μm) was used with methanol-0.2% phosphoric acid (47:53) as the mobile phase; the flow rate was 0.8 mL·min-1; UV detection wavelength was set at 254 nm and the column temperature was 25℃. Result: Ellagic acid showed good linear relationship within 0.030 6-0.551 2 μg (r=0.999 8) and its average recovery rate was 101.25%, RSD 0.9%. 3,3-di-O-methylellagic acid showed good linear relationship within 0.018 9-0.339 5 μg (r=0.999 8) and its average recovery rate was 101.34%, RSD 1.5% (n=6). Conclusion: The ellagic acid and 3,3-di-O-methylellagic acid were obtained from M. cochinchinensis for the first time, and the HPLC method was established for content determination. This method was simple, rapid and with great stability and repeatability, which could be used to determine the contents of above two components from M. cochinchinensis.关键词：Macrosolenco chinchinensis;ellagic acid;3,3-di-O-methylellagic acid;extraction and separation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Qingchang Huashi decoction (QHD) on the expressions of peroxidase body growth activated receptor-γ(PPAR-γ) and nuclear factor-κB(NF-κB) p65 in colonic mucosa of rats with ulcerative colitis, and study the mechanism of Qingchang Huashi decoction in rats with ulcerative colitis. Method: Eighty male Wistar rats were divided randomly into eight groups:normal group (ig NS 2 mL·d-1), model group (ig NS 2 mL·d-1), QHD low-dose group, QHD medium-dose group, QHD high-dose group (ig QHD 8, 16, 32 g·kg-1), SASP group (ig SASP 0.67 g·kg-1), SASP+BADGE group (ig SASP 0.67 g·kg-1+ip BADGE 20 mg·kg-1), and QHD medium-dose+BADGE group (ig QHD 16 g·kg-1+ip BADGE 20 mg·kg-1), with 10 rats in each group. TNBS was used in all of the groups except for normal group to establish the ulcerative colitis model in rats. SASP and BADGE that is the inhibitor of PPAR-γ were used as positive control drug. The general conditions and the colonic pathology morphology were observed. Western blot and Real-time PCR were performed to detect the protein and mRNA expressions of PPAR-γ and NF-κB p65.The expressions of mucoprotein2(MUC2) and trefoil factor3(TFF3) in colonic mucosa extract were detected by the method of ELISA. Result: Obvious inflammation and ulceration were observed in model group, which suggested that the model was successful. The protein and mRNA expressions of PPAR-γ in the model group were significantly lower than the normal group (P<0.01). Compared with the normal group, expressions of PPAR-γ in QHD and SASP increased slightly (P<0.05), however, these effects were reduced when QHD and SASP were combined with BADGE (P<0.05). At the same time, the protein and mRNA expressions of NF-κB p65 increased significantly in model group (P<0.01). QHD could decline the expression of NF-κB (P<0.05), while QHD combined with BADGE could not inhibit its expression any more (P<0.05). Moreover, SASP and QHD were able to increase the expressions of MUC2 and TFF3 in colonic mucosa, but these effects were weakened when combined with BADGE. Conclusion: Qingchang Huashi decoction can improve general conditions and colonic inflammation of TNBS-induced model rats. Its mechanisms may be that Qingchang Huashi decoction can activate PPAR-γ signaling pathway by inhibiting inflammation and expression of NF-κB p65, improving secretion of MUC2 and TFF3 at mucous membrane, and restoring the intestine barrier.关键词：ulcerative colitis;Qingchang Huashi decoction;peroxisome proliferators activated receptor-γ(PPAR-γ);nuclear factor-κB p65(NF-κB p65);mucoprotein 2(MUC2);trefoil factor 3(TFF3)
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发布时间：2024-01-04 - 摘要：Objective: To explore the toxic effects of total alkaloids and the simulation-processed product of Aconitine Radix in isolated hearts of rats. Method: Langendorff's heart perfusion models of SD rats were established to observe the effects of the drugs. Total alkaloids of Aconitine Radix and its simulation-processed products treated at different time points (0, 20, 40, 60, 80, 120 min) and different doses (0, 50, 100, 150, 300 μg) were adopted to intervene the perfusion model. The hemodynamics parameters were observed, including left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP), intraventricular pressure maximum upstroke velocity (+dp/dt), intraventricular pressure maximum decrease velocity (-dp/dt) and heart rate (HR). Result: Compared with the 0 min group, the 80 min simulation processing group showed cardiac toxicity at the dose of 100 μg, and 100 min and 120 min simulation processing groups showed cardiac toxicity at the dose of 300 μg (P<0.05). Both total alkaloids and simulation-processed products of Aconitine Radix can increase HR, significantly decrease LVSP, LVDP,±dp/dt, and increase LVEDP at the same time, with positive correlation with accumulated dosage. Conclusion: Both total alkaloids and simulation-processed products of Aconitine Radix show heart toxicity, which declines with the extension of the processing time.关键词：total alkaloids of Aconitine Radix;simulation-processed products of Aconitine Radix;isolated heart of rat;heart toxicity;left ventricular systolic pressure (LVSP);left ventricular end-diastolic pressure (LVEDP);left ventricular developed pressure (LVDP);+dp/dt;-dp/dt
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of water extract of Clematidis Radix et Rhizoma on proliferation of HTh74Rdox cells and its mechanism. Method: HTh74Rdox cells were treated by different concentrations of water extract of Clematidis Radix et Rhizoma, in order to observe morphological changes and apoptosis of HTh74Rdox cells. methylthiazolyldiphenyl-tetrazolium bromide (MTT) was used to test the inhibitory effect of water extract of Clematidis Radix et Rhizoma on proliferation of HTh74Rdox cells. The cell cycle change and the cell apoptosis rate were further detected by flow cytometry; Western blot was used to measure the expressions of cleaved-Caspase-3, Caspase-3, Bcl-2 and Bax. Result: Zedoary oil played a significant role in inhibiting the growth of HTh74Rdox cells. The combination of water extract of Clematidis Radix et Rhizoma and adriamycin could reduce the average dose of Adriamycin. Water extract of Clematidis Radix et Rhizoma significantly increased the percentage of cleaved-Caspase-3/Caspase-3 and Bax/Bcl-2. Conclusion: Water extract of Clematidis Radix et Rhizoma can inhibit the growth of HTh74Rdox cells. And its possible mechanism may be correlated with the increase of cleaved-Caspase-3/Caspase-3 and Bax/Bcl-2 ratio. Water extract of Clematidis Radix et Rhizoma combined with chemotherapy drug has the effect in reducing toxicity and increasing efficiency.关键词：water extract of Clematidis Radix et Rhizoma;anaplastic thyroid carcinoma;growth inhibition;reducing toxicity and increasing efficiency;Caspase-3;Bcl-2;Bax
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Shenguiren mixture on behavior of postpartum depression model rats, and measure protein expression of mitogen-activated extracellular regulated kinase 1(MEK1) on extracellular signal-regulated kinase 1/2(ERK1/2) channel in hippocampus, frontallobe and hypothalamus neurons. Method: Totally 150 female rats were divided into control group, model group, high, medium and low-dose Shenguiren groups (7, 3.6, 1.8 g·kg-1·d-1), positive drug groups[sertraline group (4.5 mg·kg-1·d-1), and estradiol benzoate (0.01 mg·kg-1·d-1)]. Except for the normal group, all of the remaining groups were subcutaneously injected with estradiol benzoate and progesterone to establish the depression model. Changes in their behavioral indicators were observed. Protein expressions of p-MEK1, MEK1 in hippocampus, frontallobe, hypothalamus neurons were detected by Western blot. Result: Compared with normal group, model group showed significant decreases in weight, appetite, activity, interest, but increases in protein expression of p-MEK1 in hippocampus, frontallobe, hypothalamus neurons (P<0.01). Shenguiren mixture can improve behavioral indicators, such as weight, appetite, activity, interest of the postpartum depression model group. The depression model showed obvious decreases in protein expressions of p-MEK1, MEK1 in hippocampus, frontallobe, hypothalamus neurons. high, medium, low-dose Shenguiren groups showed obvious increases in protein expressions of p-MEK1, MEK1 in hippocampus, frontallobe, hypothalamus neurons, particularly in medium, low-dose Shenguiren groups. Conclusion: Shenguiren mixture can improve the behavioral indicators of the postpartum depression model rats, up-regulate protein expressions of p-MEK1, MEK1 of ERK1/2 signaling pathway to a certain extent, particularly in medium, low-dose Shenguiren groups. This suggests that the mechanism of Shenguiren mixture is to regulate ERK1/2 signaling pathway and alleviate symptoms of depression.关键词：Shenguiren mixture;postpartum depression;ERK1/2;p-MEK1;MEK1
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of extract of Jujubae Fructus(EFJ) on iron deficiency anemia in rats. Method: The rats were fed iron deficiency diets for 6 weeks, and randomly divided into five groups based on hemoglobin (HGB) and weight:model group, low, medium and high-dose EFJ groups (2.7, 5.4, 10.8 g·kg-1) and ferrous sulfate group (100 mg·kg-1); And a normal group was set up. After continuous oral administration for 1, 2, 4 weeks, hematologic parameters red blood cell(RBC), hematocrit(HCT), HGB, mean corpuscular volume(MCV), mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin concentration(CHCM), hemoglobin concentration distribution width (HDW), red cell distribution width(RDW), platelet(PLT), mean platelet volume(MPV) and plateletocrit(PCT) were investigated. Serum iron, total iron binding capacity, iron retention in liver and unsaturated iron binding capacity were also detected after administration for 4 weeks. Result: Compared with normal group, model group showed obvious decreases in HGB, RBC, HCT, MCH, MCHC, CHCM, increase in PLT (P<0.05, P<0.01), significant reductions in serum iron, iron saturation, total iron binding capacity, iron retention in liver, and rise in unsaturated iron binding capacity (P<0.05, P<0.01). Compared with model group, low, medium and high-dose EFJ groups showed obvious increases in HGB, RBC, HCT, MCH, MCHC, CHCM, decrease in PLT and improvements in MCV, HDW, RDW, MPV, PCT in iron deficiency diet rats. Furthermore, it can significantly increase serum iron, iron saturation, total iron binding capacity, iron retention in liver (P<0.05, P<0.01). Conclusion: Extract of Jujubae Fructus can significantly improve iron deficiency anemia.关键词：extract of Jujubae Fructus;iron deficiency anemia;hemoglobin;serum iron
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Ferulae Resina ethyl acetate extract on the migration and invasion of human colon adenocarcinoma cell line Caco-2 and its relevant mechanism. Method: With Caco-2 cells as the research object, 6.25, 12.5 mg·L-1 of Ferulae Resina ethyl acetate extract and cisplatin were respectively applied in human colon adenocarcinoma Caco-2 cells in the logarithmic phase of growth. A blank control group was set up. Scratch healing method and transwell chamber were used to observe 0-48 h Caco-2 cells migration in each group and the number of Caco-2 cell passing through the matrix membrane in 72 h in each group, and calculate Caco-2 cell migration rate and invasion rate at each time point. Gene and protein expressions of phosphatase and tensin homolog (PTEN), serine/threonine (Akt), mammalina target of rapamgcin (mTOR), and protein in Caco-2 cells were detected by Real-time PCR and Western blot. Result: Ferulae Resina and cisplatin groups showed slower cells healing degrees at 24 h and 48 h compared with the blank group (P<0.05), at 72 h, the number of cells passing through the matrix membrane was significantly less than that in blank group (P<0.01). In each dose group, PTEN gene expression levels were significantly higher, and Akt, mTOR gene expression levels were significantly lower (P<0.05). Conclusion: Ferulae Resina ethyl acetate extract can significantly inhibit the migration and invasion of human colon adenocarcinoma Caco-2 cells, which may be correlated with increase in PTEN gene expression.关键词：Ferulae Resina;Caco-cell;metastasis;invasion;phosphatase;serine/threonine (Akt) protein kinase;mammalina target of rapamgcin (mTOR)
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发布时间：2024-01-04 - 摘要：Objective: To study the correlation between the effect of Zexietang on hyperlipidemic rats and the changes in intestinal microflora. Method: Totally 40 healthy male SD rats were randomly divided into normal control group (group C), high fat diet group (group M), high fat diet plus simvastatin pretreatment group (positive control group, group Y, 2.1 mg·kg-1·d-1) and high fat diet plus Zexietang pretreatment group (group Z, 2.2 g·kg-1·d-1), with 10 in each group. After modeling for 4 weeks, both of group Y and group Z were given the corresponding drugs by gavage once a day. Group C and group M were given the same volume of normal saline. After administration for 4 weeks, the levels of serum total cholesterol(TC), total triglyceride(TG), low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) were detected. HE was performed to detect the histological changes of livers. Total DNA was extracted from the intestinal tract, and the changes of intestinal microflora were detected. Result: The levels of TC, TG, LDL-C in group M were higher than that of group C (P<0.05). After pretreatment with Zexietang for 4 weeks, the hyperlipidemic rats in group Z and group Y displayed reductions in levels of TC, TG and LDL-C compared with the model group (P<0.05). According to the histomorphological test, rats in the model group showed liver in light pink color, with tissue swelling, surface roughness and lower elasticity than the normal group; Compared with the model group, group Z and group Y showed significant improvement in liver color and elasticity. Furthermore, the intestinal bacterial 16S rDNA high-throughput sequencing analysis results showed that in the Zexietang pretreatment group, the structural diversity and richness of intestinal flora of the rats were obviously increased. Moreover, the number of normal intestinal flora was recovered to a certain extent, and the disorder of intestinal flora was alleviated. Conclusion: Zexietang can effectively regulate the disorder of lipid profiles metabolism in hyperlipidemic rats by regulating the target of intestinal flora, so as to play the role of anti-hyperlipidemia.关键词：Zexietang;intestinal microecology;hyperlipidemia;hypolipiclemtc effect
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Lycium ruthenicum fructus on the expression of MAPK signal channel protein and antioxidantive stress damage capacity in skeletal muscle of rats with excessive training. Method: Equivalent load aerobic training and high intensity endurance training rat model was established. A total of 50 49-day old male SPF Wistar rats were randomly divided into 4 groups, with 12 in each group (the rats that did not meet the requirements were removed):control group (C Group), aerobic training group (M group), overtraining group (OM group), overtraining and Lycium ruthenicum fructus group (LM group). The dose of LM group was 4.48 mg·kg-1, with ig volume of 5 mL·kg-1. The other groups were given equal volume of saline. After 42 days' exhaustive swimming training, mitogen activated protein kinase (MAPK) signal channel protein expression and antioxidantive stress damage indicators in skeletal muscle of rats were examined. Spectrophotometry was adopted to detect activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA). Western blot was used to detect MAPK signal channel protein expression. ELISA was applied in detecting content of 3-nitrotyrosine (3-NT) and 8-hydroxy-2-deoxyguanosine (8-OHdG). Acetylcysteine method was adopted to detect activity of creatine kinase (CK). Phenylhydrazine chromogenic method was used to detect activity of lactate dehydrogenase (LDH). Result: The exhaustive swimming time of M group was longer than C group, and that of LM group was significantly longer than OM group (P<0.01). The SOD activity in M group was higher than that in C group, that in OM group was significantly lower than C group and M group (P<0.05), and that in LM group was significantly higher than OM group (P<0.05). 3-NT and 8-OHdG content in M group was higher than that in C group, that in OM group was significantly higher than C group and M group (P<0.01), and that in LM group was significantly lower than OM group (P<0.01). The LDH and CK activities of serum in M group was slightly higher than C group, that in OM group was significantly higher than C group and M group (P<0.01), and that in LM group was significantly higher than OM group (P<0.05, P<0.01). The expression of p38 MAPK and ERK signal channel protein in M group was higher than that in C group (P<0.05), and that in M group was lower than the OM group (P<0.05); that in OM group was higher than C group (P<0.01); that in LM group was higher than that in C group (P<0.05), and that in LM group was lower than OM group (P<0.05). Conclusion: The supplementation of Lycium ruthenicum fructus can effectively alleviate the imbalance of the inner environment caused by overtraining, inhibit the excessive expression of MAPK signal channel protein, activate antioxidant enzyme gene expression induced by skeletal muscle MAPK signal transduction system of overtraining rats, improve the capacity of scavenging oxygen free radicals in body, especially skeletal muscle, regulate the antioxidantive stress damage capacity of body, and prevent and delay the oxidative stress injury and the occurrence and development of the sports fatigue.关键词：Lycium ruthenicum fructus;overtraining;mitogen activated protein kinase signaling pathway;protein expression;antioxidantive stress damage
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects and mechanism of Buyang Huanwu Tang (BHT) on vascular endothelial cells (VEC) injured by oxidized low density lipoprotein (ox-LDL), and compare the effect between drug-containing serum and drug-containing plasma on VEC. Method: The modified BHT-containing serum and plasma were prepared. Human umbilical vascular endothelial cells (HUVECs) were treated with serum and plasma containing modified BHT and incubated with ox-LDL for additional 24 hours for ox-LDL modeling. The cell viability was observed by MTT assay, and cells apoptosis rate was observed by using Hoechst 33258 staining and flow cytometry; meanwhile the levels of nitric oxide (NO), malondialdehyde (MDA), and the activity of superoxidedismutase (SOD), glutathione peroxidase (GPx) were determined to explore the difference in the protective effect between modified BHT-containing serum and plasma on injured VEC. Result: As compared with the blank control group, the percentage of cells death and apoptosis was significantly higher in the model group (P<0.05). As compared with the model group, the percentage of cells death and apoptosis was significantly reduced in modified BHT-containing serum group and plasma group (P<0.05); cell viability was significantly increased(P<0.05); the levels of NO, SOD and GPx were all significantly increased, the level of MDA was significantly reduced (P<0.05). Besides, the percentage of cells death and apoptosis was lower in modified BHT-containing serum group than that in plasma group (P<0.05); however, the level of NO in modified BHT-containing plasma group was significantly higher than that in serum group (P<0.05). Conclusion: The serum and plasma containing modified BHT could prevent VEC from oxidative injury induced by ox-LDL, and the mechanism may be associated with improving the function of endothelial cells, anti-oxidative damage and inhibiting cell apoptosis. The protective effect of modified BHT-containing plasma on VEC was more stronger than the modified BHT-containing serum under certain conditions.关键词：serum and plasma containing modified Buyang Huanwu Tang;human umbilical vascular endothelial cells;oxidized low density lipoprotein;nitric oxide
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of total extract from persimmon parasitism (EPP) in diabetic mice induced by streptozotocin (STZ). Method: EPP was extracted and diabetic mice models were established by tail vein injection of STZ (120 mg·kg). Diabetic mice were randomly divided into 5 groups:EPP high dose group, EPP middle dose group, EPP low dose group, model control group, and metformin(10,5,2.5 g·kg-1)group; another 10 healthy mice were considered as normal control group. After the mice were treated with corresponding drugs for 14 days, their fasting blood glucose (FBG), the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malonaldehyde (MDA) levels in serum and liver tissues, as well as lipid metabolism were determined(P<0.05). The pathological changes of pancreas tissues were observed through HE staining. Result: As compared with the model control group, EPP could significantly decrease the blood glucose in STZ-induced diabetic mice and decrease MDA content, but significantly increase the levels of SOD and GSH-Px in serum and liver tissues; reduce the levels of cholesterol (TC, triglyceride (TG), and low density lipoprotein (LDLC) in serum; increase the level of high density lipoprotein (HDLC). In addition, there was an improvement on pathological changes of pancreas in EPP groups. Conclusion: EPP can significantly reduce fasting blood glucose level in diabetic mice induced by STZ, improve the ability of anti-oxidation and lipid metabolism.关键词：total extract from persimmon parasitism;diabetes mellitus;blood glucose;lipid;anti-oxidant
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发布时间：2024-01-04 - 摘要：Objective: To investigate the relaxation effects and mechanisms of n-butanolic fraction of Lemonfragrant Angelica Root (LAR) on rat thoracic aorta. Method: Thoracic aortic ring perfusion in isolated ratswas used to observe the effect of n-butanolic fraction on tension changes of aorta rings preshrunk by KCl and norepinephrine (NE). In Ca2+-free kreb's solution, the effects of n-butanolic fraction on Calcium chloride concentration-response curves and the tension changes shrunk by NE were investigated. Confocal laser scanning microscope was used to detect the changes of smooth muscle cells (VSMCs)[Ca2+]i. Result: The n-butanolic fraction of LAR relaxed the KCl and NE-induced contraction of aortic rings, either with or without intact endothelium, in a concentration-dependent manner, and there was no effect on relaxation after after endothelium-denuding. In Ca2+-free solution, n-butanolic fraction of LAR significantly reduced the CaCl2 and NE-induced contraction of aortic rings. Under confocal laser scanning microscope, n-butanolic fraction of LAR (1.22 g·L-1) could inhibit the increase of smooth muscle cells (VSMCs)[Ca2+]i induced by KCl, NE and thapsigargin (TG). Conclusion: n-Butanolic fraction of LAR may relax the tension of aorta rings, probably by inhibiting the influx calcium and calcium release. This study provided evidence for the production techniques of LAR decoction after steam distillation and separating the water-soluble efficacy mark components.关键词：n-butanolic fraction of LAR;thoracic aorta rings;VSMCs;confocal laser scanning;mechanisms
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective effect and mechanism of active components compatibility of ethyl acetate extracts(HE) and fatty oil(HSO) of Herpetospermum caudigerum on immunologic liver injury induced by concanavalin A (ConA) in mice. Method: The 80 mice were randomly divided into 8 groups (n=10), normal group, model group, biphenyl double ester group (positive control), HSO group,HE group, HSO and HE of 1:1, 1:2, 2:1 ratio groups. After ig administration of ethyl acetate extracts,fatty oil and different compatibilities for 8 days in treatment group, the mice were injected with ConA (30 mg·kg-1) through tail vein to establish the model of immunological liver injury. Blood was collected after 8 hours modeling to prepare liver homogenate. Liver and spleen indexes were calculated; the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and nitrogen monoxidum (NO) in serum, superoxide dismutase (SOD), malondialdehyde (MDA) in liver homogenate were assayed by ELISA; and pathological morphological changes in liver cells were monitored by HE. Result: Ethyl acetate extracts, fatty oil and their combinations of H. caudigerum could decrease liver and indexes, the levels of ALT, AST, NO, MDA and increased SOD activities. Meanwhile, the pathological damage in liver was relieved. As compared with the model group, thefatty oiland ethyl acetate extracts at 2:1 ratio group showed the best effect on immunological liver injury in mice, with significant differences in above indexes. Conclusion: Ethyl acetate extracts compatibility with oil can protect immunological liver injury in mice, and the effect was superior to that of single component. The mechanism may be associated with anti-inflammatory, anti-oxidation and scavenging oxygen free radical.关键词：Herpetospermum caudigerum;ethyl acetate extracts;fatty oil;component compatibility;immunologic liver injury
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发布时间：2024-01-04 - 摘要：Objective: To study the Effect of Qianjin Gukang powder on tibia fracture model of rats. Method: Forty male SD rats with weight between 180-220 g, were selected. Except for the 10 rats in normal control group, the rest of 30 rats were injected with 2% pentobarbital sodium for anesthesia. Under aseptic conditions, the left lower limb tibia was cut at about 1 cm below tuberosity with bone shear to make 3 mm-wide and 2 mm-deep bone defect, so as to establish the tibia fracture model. The model rats were randomly divided into three groups, namely model group, and Qianjin Gukang powder low and high-dose groups (0.35, 0.7 g·kg-1). Each group was respectively given drugs, with the dosing volume of 15 mL·kg-1, one time a day, for 21 days in a row. After the final administration, efforts were made to make X-ray imaging for the left tibia, collect blood for blood rheological test, and take the tibia bone scabs parts for histopathological examination. Result: Compared with normal group, model group showed mild callus changes, malposition, significantly increase in blood viscosity, and obvious pathological changes in fracture callus parts. Compared with model group, X-ray imaging showed Qianjin Gukang powder low and high-dose groups could obviously promote the fracture healing (P<0.05,P<0.01), high-dose group could obviously reduce blood viscosity of rat tibia fracture model (P<0.05); both Qianjin Gukang powder low and high-dose groups can significantly transform histopathological changes in callus parts after fracture (P<0.05, P<0.01). Conclusion: Qianjin Gukang powder has a certain effect on promoting healing of rat tibia fractures.关键词：Qianjin Gukang powder;fracture model;blood viscosity
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of modified Shoutaiwan for threatened abortion with uterine hemorrhage in early pregnancy, and investigate its mechanism. Method: The 146 patients with threatened abortion with uterine hemorrhage in early pregnancy who were dialectical to deficiency of the kidney and blood stasis in traditional Chinese medicine (TCM) were randomly divided into treatment group and control group. Patients in control group took dydrogesterone tablets orally and progesterone by intramuscular injection. Patients in treatment group also received modified Shoutaiwan based on the treatment in control group. The treatment course lasted for ten days in both groups, and followed up until thenuchal translucency (NT) examination. The clinical efficacy, TCM symptom scores, progesterone (P) and estrodiol (E2) in serum and the result of NT were compared between two groups. Result: The cumulative effective rate was 84.2%in treatment group, higher than 70.0% in control group (P<0.05). All of the TCM symptom scores in the treatment group were much lower than those in the control group after treatment (P<0.01). The level of P in serum in the treatment group was higher than that in control group after treatment (P<0.01). There was no statistically significant difference in NT results between two groups and the use of modified Shoutaiwan did not cause the increased rate of fetal abnormalities. Conclusion: Modified Shoutaiwan can safely and effectively treat the patients with threatened abortion with uterine hemorrhage in early pregnancy who were dialectical to the deficiency of kidney and blood stasis in traditional Chinese medicine.关键词：Shoutaiwan;early pregnancy;threatened abortion;uterine hemorrhage;progesterone(P);estrodiol (E2);thenuchal translucency (NT)
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shenshuaining tablet on patients' prognosis during chronic kidney disease (CKD) at CKD3-4 stage and levels of transforming growth factor-β1 (TGF-β1), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px). Method: Totally 132 patients at CKD3-4 stage were randomly divided into observation group (67 cases) and control group (65 cases) by random number table. Patients in control group got comprehensive western medicine therapy, including controlling blood lipid, blood pressure and blood glucose, correctting anemia and calcium-phosphorus metabolism disorder, and were provided with compound keto acid tablets during the meal, 6-8 tablets/time, 3 times/day, and losartan potassium tablets, 100 mg/time, 1 time/day. In addition to the therapy of control group, patients in observation group were also given Shenshuaining tablet, 6 tablets/time, 3 times/day. Courses of treatment were 12 months, or until the next dialysis treatment at CKD5 period. Patients' conditions from CKD3 to CKD4 and from CKD4 to CKD5 were recorded. Before and after treatment, scores of traditional Chinese medicine(TCM) syndrome and quality of live were recorded. And levels of blood urea nitrogen (BUN), serum creatinine (SCr), hemoglobin (Hb), plasma albumin (Alb), 24 hours' urine protein quantitation (Upr), eGFR, TGF-β1, IL-6, TNF-α, SOD and GSH-Px were detected before treatment and at the 6th and 12th month after treatment. Result: The total effect rate of TCM symptoms in observation group was 88.06%, which was higher than 70.77% in control group (P<0.05). The total effect rate of disease in observation group was 85.07%, which was higher than 67.69% in control group (P<0.05). And incidence rate of outcome event in observation group was 29.85%, which was lower than 49.23% in control group (P<0.05). After treatment, scores of SF-36 scale in observation group increased (P<0.01), which was higher than that in control group (P<0.01) in the same period. At the 6th month after treatment, levels of Alb, Hb and eGFR in observation group were higher than those in control group, and level of SCr was lower than that in control group (P<0.05). At the 12th month after treatment, levels of Alb, Hb and eGFR were higher than those in control group (P<0.01), and BUN and SCr were lower than those in control group (P<0.05 or P<0.01). Compared with the data before treatment, there were statistical significant differences between two groups at the 6th and 12th months after treatment (P<0.01), and levels of TNF-α, IL-6 and TGF-β1 were higher than those in control group, and levels of SOD and GSH-Px were higher than those in control group (P<0.05 or P<0.01). Conclusion: In addition to the routine western medicine therapy, Shenshuaining tablet can delay course of disease of patients at CKD3-4, and improve clinical effect and quality of live. Its mechanism of action may be correlated with relief in inflammatory response and improvement of antioxidant capacity.关键词：chronic kidney disease;prognosis;Shenshuaining tablet;transforming growth factor-β1 (TGF-β1);interleukin-6 (IL-6);tumor necrosis factor-α (TNF-α);superoxide dismutase;glutathion peroxidase
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发布时间：2024-01-04 - 摘要：Objective: To discuss the analgesic effect of modified Guizhi Fuzi Tang combined with intermediate frequency induction therapy in treating scapulohumeral periarthritis with cold dampness, and the mechanism of action on recovery of shoulder joint function. Method: One hundred and thirty-six patients were randomly divided into control group (68 cases) and observation group (68 cases) by random number table. Both groups took shoulder functional exercise according to guide. Patients in control group were also provided intermediate frequency induction therapy in continuous mode, 20 min/time, 1 time/day, 6 times/week. And patients in observation group were also given modified Guizhi Fuzi Tang combined with intermediate frequency induction therapy, 20 min/time, 1 time/day, 6 times/week. Pain was evaluated by MeGill Pain Questionnaire (SF-MPQ). Shoulder joint function was scored by Constant-Murley. Daily activities were evaluated by American Shoulder and Elbow Surgeons' Form (ASES) shoulder joint function evaluation scale. Before and after treatment, levels of substance P (SP), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2) were detected. And cold dampness was scored. Result: The total rate of clinical effect in observation group was 95.31%, which was higher than 81.97% in control group (P<0.05). The three dimensions of SF-MPQ and the total score and cold dampness in observation group were lower than those in control group (P<0.01). And scores of pain, activities of daily living and range of motion and the total scores in observation group were higher than those in control group (P<0.01). After treatment, levels of SP, TNF-α and PGE2 were lower than those in control group (P<0.01), and level of IL-10 was higher than that in control group (P<0.01). Conclusion: In addition to functional exercise, modified Guizhi Fuzi Tang combined with intermediate frequency induction therapy can relieve pain, ameliorate cold dampness, improve function of joint and joint range of motion, and the mechanism of action may be regulating inflammatory mediators and reducing levels of SP and PGE2.关键词：scapulohumeral periarthritis;cold dampness;Guizhi Fuzi Tang;intermediate frequency induction;MeGill Pain Questionnaire;Constant-Murley scale;American Shoulder and Elbow Surgeons' Form (ASES) shoulder joint function evaluation scale
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Danhong Huayu oral on levels of vascular endothelial growth factor (VEGF), angiopoietin protein and angiopoietin in patients of non-proliferative diabetic retinopathy (NPDR) with collaterals stagnation syndrome. Method: One hundred and twenty-nine NPDR patients were randomly divided into control group (64 cases, 115 eyes) and observation group (65 cases, 116 eyes) by random number table. Patients in control group took dobesilate calcium capsules, 0.5 g/time, 3 times/day, pancreatic kallikrein enteric-coated tablets, 1 tablet/time, 3 times/day. In addition to the therapy of control group, observation group was also given Danhong Huayu oral, 20 mL/time, 3 times/day. Courses of treatment were 12 weeks. Vision, intraocular pressure and fundus examination after dilated were detected. Scores of collaterals stagnation syndrome were graded. Optical coherence tomography (OCT) and fundus fluorescein angiography were conducted. Before and after treatment, levels of Angiopoietin-2 (Ang-2), Angiopoietin-1 (Ang-1), Angiopoietin protein-3 (ANGPT-3), Angiopoietin protein-4 (ANGPT-4) and VEGF were detected. Result: After treatment, vision in observation group was better than that in control group (P<0.05), and scores of macular edema and ollaterals stagnation syndrome were lower than those in control group (P<0.01). Foveal thickness and macular volume in observation group were lower than those in control group (P<0.05). Retinal vascular leakage area and etinal capillary non-perfusion area in observation group were less than those in control group (P<0.01), the number of tumor microvessels and arm retinal circulation time were less and shorter than those in control group (P<0.05). Levels of VEGF, ANGPT-3 and Ang-2 were lower than those in control group, while ANGPT-4 and Ang-1 were higher than those in control group (P<0.01). Conclusion: Danhong Huayu oral can improve patients' vision, relieve edema caused by macular, ameliorate retinal circulatory disorders, and regulate VEGF, ANGPT-3/ANGPT-4 and Ang-1/Ang-2, inhibit generating new blood vessels, and delay progression of NPDR.关键词：non-proliferative diabetic retinopathy;collaterals stagnation syndrome;Danhong Huayu oral;vascular endothelial growth factor (VEGF);angiopoietin protein;angiopoietin
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of compound Huanglu oral liquid in treating chronic hepatitis B(CHB) syndrome of damp-heat stagnation and investigate its effects on levels of interferon-γ (IFN-γ), interleukin-10 (IL-10), IL-21 and IL-22. Method: One hundred and forty-six CHB patients were randomly divided into control group (72 cases) and observation group (74 cases) by random number table. Patients in control group took entecavir tablets, 0.5 mg/time, 1 time/day. Based on the treatment in control group, the patients in observation group added compound Huanglu oral liquid, 20 mg/time, 3 time/day. The treatment course was 48 weeks in both groups. Before treatment, and at the 24th week and 48th week, the levels of Hepatitis B e antigen (HBeAg) and human hbv gene (HBV-DNA) were detected. For every 3 months, the levels of glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST) and serum total bilirubin (TBIL) were detected. Scores of syndrome of damp-heat stagnation were graded before and after treatment. Levels of IFN-γ, IL-10, IL-21 and IL-22 were detected before and after treatment. Result: Response rate was 89.19% in observation group, higher than 73.61% in control group at the 48th week after treatment (χ2=5.875, P<0.05). The total effective rate for traditional Chinese medicine(TCM)symptoms was 86.49% in observation group, higher than 58.33% in control group (χ2=14.541, P<0.01). Negative rate of HBeAg was 41.89% in observation group, higher than 26.39% in control group (χ2=3.895, P<0.01). Negative rate of HBV-DNA was 82.43% in observation group, higher than 68.06% in control group (χ2=4.06, P<0.05). After treatment, the levels of AST, ALT and TBIL in observation group were lower than those in control group (P<0.01). Complex constant effect of ALT was 86.49% in observation group, higher than 72.22% in control group after treatment (χ2=4.548, P<0.05). The complex constant effect of AST was 89.19% in observation group, higher than 75% in control group (χ2=5.019, P<0.05). Levels of IFN-γ and IL-21 in observation group were higher than those in control group, levels of IL-10 and IL-22 were lower than those in control group (P<0.01). Conclusion: compound Huanglu oral liquid combined with entecavir tablets can increase biochemistry, viral and serological response, ameliorate TCM symptoms, regulate immune function and improve the body's virus removal ability in the treatment of CHB, so it is worthy to be used in clinical.关键词：chronic hepatitis B;syndrome of damp-heat stagnation;compound Huanglu oral liquid;hepatitis Beantigen;interferon-γ;interleukin-10;interleukin-21;interleukin-22
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Xiao Huoluodan combined with Guizhi Shaoyao Zhimu Tang in treating rheumatic arthritis with stagnancy of cold-dampness and investigate its effects on levels of tumor necrosis factor-α(TNF-α), interleukin-17, 27, 32 (IL-17, 27, 32). Method: One hundred and twenty-two patients were randomly divided into western medicine group (61 cases) and combination group (61 cases) by random number table. Another 15 healthy subjects were selected as healthy group. Patients in western medicine group took nimesulide tablet, 0.1 g/time, 2 times/day; leflunomide capsule, 20 mg/time, 1 time/day. Based on the treatment in western medicine group, patients in combination group added Xiao Huoluodan combined with Guizhi Shaoyao Zhimu Tang, 1 dose/day. The treatment course was 3 months for both groups. Before and after treatment, tender joint count, pain, swelling, inhibited bending and stretching, morning stiffness and health assessment questionnaire (HAQ) were recorded; scores of stagnancy of cold-dampness were graded; levels of erythrocyte sedimentation rate (ESR), C reactive protein (CRP), rheumatoid factors (RF), IL-17, IL-27, IL-32 and TNF-α. Result: After treatment, evaluation criteria for rheumatoid ACR20 was 90.16% in combination group, higher than 68.85% in western medicine group (χ2=8.502, P<0.01). ACR50 and ACR70 in combination group were higher than those in western medicine group, but there were no statistically significant differences between two groups. The total effective rate for traditional Chinese modicine(TCM) symptom was 88.52% in combination group, higher than 67.21% in western medicine group (χ2=8.038, P<0.01). The scores of tender joint count, pain, swelling, inhibited bending and stretching, morning stiffness and health assessment questionnaire (HAQ), disease activity score in 28 joints(DAS28) scores, and levels of ESR, CRP and RF in combination group were lower than those in western medicine group after treatment (P<0.01). As compared with the healthy group, levels of IL-17, IL-27, IL-32 and TNF-α were higher in case groups before treatment (P<0.01). And after treatment, those levels in combination group were lower than those in western medicine group(P<0.01), but still higher than those in healthy group (P<0.01). Conclusion: Xiao Huoluodan combined with Guizhi Shaoyao Zhimu Tang can ameliorate symptoms, control disease activity, improve clinical efficacy, and down-regulate the levels of IL-17, IL-27, IL-32 and TNF-α in the treatment of rheumatic arthritis with stagnancy of cold-dampness.关键词：rheumatic arthritis;stagnancy of cold-dampness;Xiao Huoluodan;Guizhi Shaoyao Zhimu Tang;interleukin-17;interleukin-27;interleukin-32
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发布时间：2024-01-04 - 摘要：Objective: To explore the prescription rules of traditional Chinese medicine in treatment of AIDS and provide objective basis for Chinese medicine treatment of AIDS. Method: Complex network analysis method was used to retrospectively study 2 674 Chinese herbal prescriptions collected from January 2010 to April 2010 in Henan, Anhui, and Hubei. Then the core herbs, compatibility rules and characteristics of commonly used prescriptions for AIDS were analyzed. Result: Multidimensional retrieving results showed that the core TCM herbs for AIDS mainly included Atractylodis Macrocephalae Rhizoma, Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle, Ginseng Radix et Rhizoma, Jujubae Fructus, Glycyrrhizae Radix et Rhizoma, Astragali Radix, Dioscorea Rhizoma, Codonopsis Radix, Lablab semen Album and other Qi tonifying herbs; Angelicae Sinensis Radix, Paeoniae Radix Alba, Rehmanniae Radix Praeparata and other blood tonifying herbs; Poria, Coicis Semen, Amomi Fructus, Alismatis Rhizoma and other dampness clearing herbs. Core prescriptions included Buzhong Yiqi Tang, Xiangsha Liujuntang, and Shenling Baizhu San. Conclusion: The pathogenesis of HIV/AIDS was shown as Qi-blood deficiency and dampness, so the treatment should be replenishing Qi, invigorating spleen and clearing dampness. The results in complex network analysis method in the scientific research and medical records of this paper were basically consistent with the clinical application.关键词：AIDS;core herbs;prescription rules;complex network analysis
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发布时间：2024-01-04 - 摘要：Due to short time delay,good penetration enhancing effect and low toxicity,volatile oil from Chinese medicine have attracted more and more attention of scholars both at home and abroad.In this paper,the skin penetration enhancing effect of volatile oil(used alone,used together with other penetration enhancer,the penetration enhancing effect of their constituents,penetration enhancing mechanism) and their skin penetration effect were reviewed.The problems existing in this area were pointed out,model drugs were mostly chemical drugs,the evaluation indicators were single chemical composition,the guiding role of theory of traditional Chinese medicine was weakened;studies on the penetration material basis,the penetration enhancing mechanism,the penetration enhancing law were very few;studies on the influencing factors in vitro diffusion cell method was not comprehensive.Meanwhile,the research ideas are put forward,which are suitable for Chinese medicine,on the basis of studying the traditional literature,taking classic prescriptions or commonly used drug pairs as the object of study and combination of chemical composition and biological effect as indexes,experiment should be carried out by combination of in vitro diffusion cell and in vivo method.关键词：Chinese medicine;volatile oil;skin penetration enhancing effect;skin penetration effect;biological effect;diffusion cell
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发布时间：2024-01-04 - 摘要：Primary immune thrombocytopenia(ITP),which characterizes platelets decreasing, the body bleeding, the skin mucous membrane blood stasising and purple, epistaxis, and visceral hemorrhage, is an autoimmune disease. ITP animal model is the main method of studying the pathogenesis and treatment of ITP, and has played an important role in the study of ITP. Therefore, how to create and close to the clinical animal model is the key issue in the field of ITP research. At present, there are many methods to create the animal model of ITP, such as injecting anti-plateletserum or platelet, transgenic models, Spontaneous immune models, transgenic modeling and other modeling methods. Immunological modeling is the most commonly used method, the experimental animals are mainly mice, commonly used injection is intraperitoneal injection, commonly used immunogen anti-platelet serum, monoclonal/polyclonal antibodies, platelets, spontaneous model and transgenic model use less. In addition disease and syndrome combination models with heat syndrome, Kidney yin deficiency syndrome and syndrome of qi failing to control blood. The research progress of ITP in disease models and model of disease and syndrome combination will be summarized and analyzed in this article,and to provide reference for further study of ITP animal models.关键词：primary immune thrombocytopenia;animal model;combination of disease and syndrome
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发布时间：2024-01-04 - 摘要：Autophagy is a vital programmed cell death mode widely existing in the eukaryotic cells. During the process, cytoplasmic proteins and organelles are wrapped by single or double-membrane auto-phagosomes, and then integrated with lysosomes into auto-lysosomes, so as to finally degrade the wrapped content, and achieve self-digestion and organelles renewal. Autophagy gets involved in a wide variety of physiological and pathological processes and closely related to tumorigenesis. Under normal circumstances, autophagy could clear misfolded proteins and organelles, leading to the suppression of stress reaction and downregulation of cancer incidence. However, although autophagy is primarily a protective metabolic pathway for cells, it can also promote tumor viability by degrading abnormal proteins and organelles in cancer cells. In recent years, more and more experimental evidences have indicated that active components of traditional Chinese medicine(TCM), like alkaloids, flavonoids, saponins and cardiac glycosides, could induce autophagy of tumour cells. In this review, based on active components of TCM medicine and molecular mechanisms of autophagy in cancer cells, we summarize the research progress of autophagy induced by TCM, in order to provide reference for the prevention and treatment of tumour with TCM.关键词：traditional Chinese medicine;tumour cell;autophagy;alkaloids;flavonoids;Saponins;cardiac glycosides
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发布时间：2024-01-04 - 摘要：Polygonati Rhizome,as clinical commonly used tonic medicine,has a long medical history.Its processing study on the change of chemical compositions and pharmacological efficacy in recent years have achieved some new progress.In this article,a lot of research on Polygonati Rhizoma in the past decade were summarized form processing technology,change of chemical compositions before and after processing,effect of processing on pharmacological action and so on.The chemical compositions and pharmacological action of Polygonati Rhizoma were both changed before and after processing.Considering the application situation of Polygonati Rhizoma in recent years and the safety of clinical medication,in the use of process,quality of processed products should be in the first position.关键词：Polygonati Rhizoma;processing technology;chemical compositions;pharmacological action;5-hydroxymethylfurfural;polysaccharide;Saponins
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发布时间：2024-01-04 - 摘要：Tracing the origin and development of Euodiae Fructus processing,analysing of the evolution of the processing method and functions of Euodiae Fructus,are used to lay a foundation for the further research of Euodiae Fructus.Sorting and summarizing the processing methods and relevant functions of Euodiae Fructus systematically through ancient books,such as salt,vinegar,wine,Coptidis Rhizoma,Glycyrrhizae Radix et Rhizoma processing and so on,found that different processed products play different clinical roles.Processing by Glycyrrhizae Radix et Rhizoma can reduce toxicity,processing by Coptidis Rhizoma can drop the inverse anti-nausea,processing by vinegar can relieve the pain by evacuating the liver depression,processing by Zingiberis Rhizoma Recens can eliminate cold to stop vomiting,processing by salt can guide the medicine into the kidney,processing by wine can alleviate the pain on account of Qi-depression of the heart and abdomen,etc.It can be seen that the effect of different processed products of Euodiae Fructus has obvious difference,and it is very important to select different processing methods for the research.So the history research of processing of Euodiae Fructus can provide the basis for clinical practice of its different processed products.关键词：Euodiae Fructus;processing method;functions;historical evolution;Coptidis Rhizoma;Glycyrrhizae Radix et Rhizoma
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发布时间：2024-01-04 - 摘要：To sort out and summarize the relevant literature on the protection mechanism of traditional Chinese medicine(TCM) active ingredients with the neuroprotective effects. TCM active ingredients, nervous system disease, neuroprotective effects were used as the keywords to search for the relevant literature on internal and abroad studies of neuroprotective mechanism in recent years, and then the analysis was conducted. The results showed that TCM active ingredients could suppress the neurotoxicity(against the nerve damage of Amyloid β -peptide and excitatory amino acids, improve the acetylcholine protection), the levels of inflammation (reduce the level of interleukin-6 and tumor necrosis factor-alpha and gamma interferon)and oxidative stress damage(increase the activity of Superoxide dismutase, catalase and glutathione peroxidase, lower the level of malondialdehyde), inhibit the cellular proliferation and apoptosis(increase the apoptosis protein expression of Bcl-2, decrease the apoptosis protein expression of Bax), promote angiogenesis(raise angiotensin 1 and the expression of specific tyrosine kinase receptor Tie-2) and nerve growth repair(promote the secretion of vascular endothelial growth factor and chemokines cell matrix derived factor-1) that the mechanisms had neuroprotective effects. This research through the summary and analysis that explore new research ideas and the selection of drugs for nervous system disease of the nerve protective effect to offer new drug development and clinical treatment.关键词：traditional Chinese medicine active ingredients;nerve protection;mechanism of action
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发布时间：2024-01-04
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