最新刊期
      卷 ， 期 1 ， 2007
      • WANG Xue-yong,CUI Guang-hong,HE Xi-rong,HUANG Lu-qi
        Vol. 13, Issue 1, Pages: 1-4(2007)
        摘要：Objective:To establish RP-HPLC fingerprint for adventitious root of Radix Salviae Miltiorrhizae and primarily investigate effects of different mental ion on it.Method:The Methanol extraction of Radix Salviae Miltiorrhizae was filtered through 0.45 mm filters and separated by using a Resolve TM C18 column(Waters,150 mm×3.9 mm,5 μm) at 30℃.The mobile phase consisted of acetonitrile and water was used as gradient elution for the separation.The flow rate was set at 1.0 mL·min-1 and detection was set at 270 nm.The fingerprint was then established as above condition for adventitious root of Radix Salviae Miltiorrhizae Result:11 peaks in common of 9 samples of treated with 3 kind of metal ion to adventitious root were acquired and 9 of them were chosen as symbolization for the component of Radix Salviae Miltiorrhizae.Conclution:The fingerprint profile provides a good approach to value the quality and monitor the change of content of active component in adventitious root of Radix Salviae Miltiorrhizae.  
        关键词：Adventitious root of Radix Salviae Miltiorrhizae;HPLC;Fingerprint profile;Salvia miltiorrhiza Bge   
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        发布时间：2024-09-23
      • WANG Xue-yong,CUI Guang-hong,HE Xi-rong,HUANG Lu-qi~
        Issue 1, Pages: 1-4(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.002
        摘要：Objective:To establish RP-HPLC fingerprint for adventitious root of Radix Salviae Miltiorrhizae and primarily investigate effects of different mental ion on it.Method:The Methanol extraction of Radix Salviae Miltiorrhizae was filtered through 0.45 mm filters and separated by using a Resolve TM C18 column（Waters,150mm×3.9 mm,5 μm） at 30℃.The mobile phase consisted of acetonitrile and water was used as gradient elution for the separation.The flow rate was set at 1.0 mL·min-1 and detection was set at 270 nm.The fingerprint was then established as above condition for adventitious root of Radix Salviae Miltiorrhizae Result:11 peaks in common of 9 samples of treated with 3 kind of metal ion to adventitious root were acquired and 9 of them were chosen as symbolization for the component of Radix Salviae Miltiorrhizae.Conclution:The fingerprint profile provides a good approach to value the quality and monitor the change of content of active component in adventitious root of Radix Salviae Miltiorrhizae.  
        关键词：Adventitious root of Radix Salviae Miltiorrhizae;HPLC;Fingerprint profile;Salvia miltiorrhiza Bge   
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        发布时间：2024-09-23
      • WANG Ping1,WANG Qi2,LIU Qing2,JIN Hong-yu3,LIN Rui-chao3
        Vol. 13, Issue 1, Pages: 5-7(2007)
        摘要：Objective:To compare the levels of 5 pyrethroid pesticide residues in two types Chinese traditional medicines,i.e.Ganoderma and Poria.Methods:A sum of 20 batches of Ganoderma and Poria(10 batches each)samples were collected and identified.The 5 pyrethroid pesticide residues in samples were extracted by ultrasonic washing machine with organic solvent,cleaned up by solid-phase extraction to remove the interferences of co-extractives,and then were analyzed by gas chromatograph(GC)equipped with an electron capture detector,quantified by the external standard method.Results:The 5 pyrethroid pesticide residues were not detected in 10 batches samples of Poria,Among 10 batches samples of Ganoderma,the 5 pyrethroid pesticide residues were not detected in 9 batches samples,and fenvalerate was detected in 1 batch sample without exceeding the limit.Conclusion:The result suggested that most of the Ganoderma and Poria were not contaminated by the residues of 5 pyrethroid pesticides.  
        关键词：pyrethroids;pesticide residue;Ganoderma;Poria   
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        发布时间：2024-09-23
      • WANG Ping~1,WANG Qi~
        Issue 1, Pages: 5-7(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.003
        摘要：Objective:To compare the levels of 5 pyrethroid pesticide residues in two types Chinese traditional medicines,i.e.Ganoderma and Poria.Methods:A sum of 20 batches of Ganoderma and Poria（10 batches each）samples were collected and identified.The 5 pyrethroid pesticide residues in samples were extracted by ultrasonic washing machine with organic solvent,cleaned up by solid-phase extraction to remove the interferences of co-extractives,and then were analyzed by gas chromatograph（GC）equipped with an electron capture detector,quantified by the external standard method.Results:The 5 pyrethroid pesticide residues were not detected in 10 batches samples of Poria,Among 10 batches samples of Ganoderma,the 5 pyrethroid pesticide residues were not detected in 9 batches samples,and fenvalerate was detected in 1 batch sample without exceeding the limit.Conclusion:The result suggested that most of the Ganoderma and Poria were not contaminated by the residues of 5 pyrethroid pesticides.  
        关键词：pyrethroids;pesticide residue;Ganoderma;Poria   
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        发布时间：2024-09-23
      • WANG Yang1,2,PAN Xin-gui1,RUAN Xiao-qi2,ZHANG Hong1
        Vol. 13, Issue 1, Pages: 8-9(2007)
        摘要：Objective:To develope a HPCE method for determining the content of baicalin in Xiaoyankangjun Tablets.Methods:Internal standard method was selected as quantitive method and sodium benzoate was used as internal standard.The separation was performed on a fused-silica capillary(60 cm×75 μm,effective length 45 cm).The detection wavelength was 215 nm and the applied voltage was 21 kV.10 mmol·L-1 borate(pH 9.0)was used as running buffer;Results:The linear range of the baicalin was 96～768 μg·mL-1(r=(0.999 7) and the recovery was 98.8%(RSD=1.6%).Conclusions:HPCE is a practicable method in the determination the concentration of baicalin in the Xiaoyankangjun Tablets.  
        关键词：High Performance Capillary Electrophoresis(HPCE);Xiaoyankangjun Tablets;baicalin   
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        发布时间：2024-09-23
      • WANG Yang~
        Issue 1, Pages: 8-9(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.004
        摘要：Objective:To develope a HPCE method for determining the content of baicalin in Xiaoyankangjun Tablets.Methods:Internal standard method was selected as quantitive method and sodium benzoate was used as internal standard.The separation was performed on a fused-silica capillary（60 cm×75 μm,effective length 45 cm）.The detection wavelength was 215 nm and the applied voltage was 21 kV.10 mmol·L-1 borate（pH 9.0）was used as running buffer;Results:The linear range of the baicalin was 96～768 μg·mL-1（r=（0.999 7）） and the recovery was 98.8%（RSD=1.6%）.Conclusions:HPCE is a practicable method in the determination the concentration of baicalin in the Xiaoyankangjun Tablets.  
        关键词：High Performance Capillary Electrophoresis（HPCE）;Xiaoyankangjun Tablets;baicalin   
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        发布时间：2024-09-23
      • Issue 1, Pages: 10-12(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.005
        摘要：目的:研究制定苓栀解郁片质量控制的定性定量方法。方法:采用薄层色谱法和高效液相色谱法对苓栀解郁片中酸枣仁、远志进行定性鉴别,对栀子中的栀子苷进行含量测定。结果:酸枣仁、远志薄层色谱清晰,无干扰,重复性良好。栀子苷在0.0580.928μg范围内呈良好的线性关系,r=0.999 9;回收率达到100.8%（RSD=2.5%）。结论:方法简便,专属性强,可作为该制剂的质量控制方法。  
        关键词：苓栀解郁片;酸枣仁;远志;薄层色谱;高效液相色谱;栀子苷   
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        发布时间：2024-09-23
      • Issue 1, Pages: 12-13(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.006
        摘要：目的:对红花散瘀胶囊中羟基红花黄色素A进行含量测定。方法:高效液相色谱法,样品用25%甲醇超声提取;ODS柱;以甲醇-乙腈-0.7%磷酸溶液（26∶2∶72）为流动相;检测波长403 nm;流速:1.0 mL/min;柱温:25℃。结果:羟基红花黄色素A获良好分离,在0.161.60μg范围内呈现良好的线性关系（r=0.999 9）,平均加样回收率为99.45%,RSD为2.10%。结论:本法简单、准确、重复性好,可用于红花散瘀胶囊中羟基红花黄色素A含量测定。  
        关键词：红花散瘀胶囊;红花;羟基红花黄色素A;高效液相色谱法   
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        发布时间：2024-09-23
      • Issue 1, Pages: 14-25(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.007
        摘要：目的:用高效液相法测定咽炎含片中黄芩苷的含量。方法:采用Hypersil C18柱（4.6 mm×250 mm,5μm）,流动相:甲醇-0.4%磷酸（46∶54）,检测波长280 nm。结果:平均加样回收率为98.9%,RSD=0.93%。结论:方法简便、快速、灵敏、专属性强,数据准确,为咽炎含片的质量控制提供了科学有效的方法。  
        关键词：咽炎含片;黄芩苷;高效液相法   
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        发布时间：2024-09-23
      • Issue 1, Pages: 15-16(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.008
        摘要：目的:对鲜地黄的化学成分进行分离、鉴定,寻找药效活性强的化合物。方法:采用化学分离手段,从鲜地黄新鲜的根茎中分离得到6个化合物。结果:分离得到6个化合物,通过波普鉴定谱分别为:毛蕊花糖苷（1）、梓醇（2）、桃叶珊瑚苷（3）、水苏糖（4）、胡萝卜苷（5）、β-谷甾醇（6）。并观察了体外毛蕊花糖苷对RAW细胞的作用。结论:毛蕊花糖苷对LPS诱导RAW细胞增殖具有一定的抑制作用。  
        关键词：鲜地黄;化学成分;鉴定;活性   
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        发布时间：2024-09-23
      • Issue 1, Pages: 17-18(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.009
        摘要：目的:建立碧云喷雾剂的定性定量测定方法。方法:采用TLC法对处方中的鹅不食草、川芎、辛夷进行鉴别;用高效液相色谱法测定黄芩苷的含量。结果:在TLC色谱中检出鹅不食草、川芎、辛夷;黄芩苷在0.1060.636μg范围呈良好的线性关系,r=0.999 9,平均回收率97.7%,RSD为1.5%。结论:所建立的方法能够用于本品的质量控制。  
        关键词：碧云喷雾剂;定性定量分析;高效液相色谱法;黄芩苷   
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        发布时间：2024-09-23
      • Issue 1, Pages: 19-22(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.010
        摘要：目的:研究苦参总碱的提取纯化工艺。方法:以总生物碱提取率为指标考察冷浸、渗漉、水煎煮三种提取工艺;以总生物碱及氧化苦参碱含量为指标,优选纯化工艺路线及阳离子树脂纯化工艺的技术参数,包括树脂用量,流速,解吸附方法等。结果:最佳提取工艺为:1%醋酸水溶液冷浸3次,以1/3（生药重）量的阳离子树脂纯化,5%氨水乙醇回流解吸附。提取物中苦参总生物碱含量可达95%以上,总生物碱的提取纯化转移率可达80%左右。结论:工艺简单,周期短,成本低,提取物中总碱转移率及纯度均高。  
        关键词：苦参;提取纯化;阳离子树脂;总生物碱;氧化苦参碱   
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        发布时间：2024-09-23
      • Issue 1, Pages: 23-25(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.011
        摘要：目的:研究咽乐含片的成型工艺。方法:以口感和在口腔内的溶散时限为指标,采用正交设计法对配方进行优化,对半成品进行质量控制,并对生产条件进行探讨。结果:以浸膏1份,混合辅料4份（葡萄糖,砂糖,淀粉,按20∶60∶20的比例混合）,混合,65℃烘干,粉碎,过100目筛,75%酒精制粒,65℃烘干,过14目筛,加1%硬脂酸镁,每100 g颗粒喷入5%薄荷脑乙醇液,压片,淡绿色85G型欧巴代包衣为优化出的成型工艺。在生产和包装贮存时环境的相对湿度应小于54%。结论:该制备工艺合理,为进一步开发提供了依据。  
        关键词：咽乐含片;成型工艺;正交设计   
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        发布时间：2024-09-23
      • MENG Yun-hui1,TU Xin2,TU Jin-wen3,GUAN Jiang-feng1
        Vol. 13, Issue 1, Pages: 26-28(2007)
        摘要：Objective:To observe protective effects of Tianma Gouteng Yin on the injury of human umbilical vein endothelin cells(HUVECs)induced by angiotensin Ⅱ(AngⅡ).Methods:The serum containing metabolic ingredients of Tianma Gouteng Yin was taken from rats.HUVECs were cultured in vitro by collagenase digestive method and identified with human von Willebrand factor immunocytochemical method.HUVECs were randomly divided into three groups:control group,AngⅡ group,AngⅡ and Tianma Gouteng Yin group.The morphology and density of HUVECs were observed with invert microscope.The content of TNF-α in supernatant medium was detected with enzyme-linked immunoadsorbent assay.The expression of peroxisome proliferator-activated receptor γ was measured by means of reverse transcription polymerase chain reaction.Results:Compared with control group,AngⅡ(10-6mol/L) could make the density of HUVECs to decrease and the secretion of TNF-α to increase.The expression of peroxisome proliferator-activated receptor γ was lowed.The serum containing metabolic ingredients of Tianma Gouteng Yin could inhibit the decrease of HUVECs and decrease the secretion of TNF-α and increasethe expression of peroxisome proliferator-activated receptor γ.Conclusion:Tianma Gouteng Yin could inhibit the injury of HUVECs induced by AngⅡ.  
        关键词：Tianma Gouteng Yin;angiotensin Ⅱ;Human umbilical vein endothelin cells;tumor necrosis factor;Peroxisome proliferator-activated receptor γ   
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        发布时间：2024-09-23
      • MENG Yun-hui~1,TU Xin~
        Issue 1, Pages: 26-28(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.012
        摘要：Objective:To observe protective effects of Tianma Gouteng Yin on the injury of human umbilical vein endothelin cells（HUVECs）induced by angiotensin Ⅱ（AngⅡ）.Methods:The serum containing metabolic ingredients of Tianma Gouteng Yin was taken from rats.HUVECs were cultured in vitro by collagenase digestive method and identified with human von Willebrand factor immunocytochemical method.HUVECs were randomly divided into three groups:control group,AngⅡ group,AngⅡ and Tianma Gouteng Yin group.The morphology and density of HUVECs were observed with invert microscope.The content of TNF-α in supernatant medium was detected with enzyme-linked immunoadsorbent assay.The expression of peroxisome proliferator-activated receptor γ was measured by means of reverse transcription polymerase chain reaction.Results:Compared with control group,AngⅡ(10-6 mol/L) could make the density of HUVECs to decrease and the secretion of TNF-α to increase.The expression of peroxisome proliferator-activated receptor γ was lowed.The serum containing metabolic ingredients of Tianma Gouteng Yin could inhibit the decrease of HUVECs and decrease the secretion of TNF-α and increasethe expression of peroxisome proliferator-activated receptor γ.Conclusion:Tianma Gouteng Yin could inhibit the injury of HUVECs induced by AngⅡ.  
        关键词：Tianma Gouteng Yin;angiotensin Ⅱ;Human umbilical vein endothelin cells;tumor necrosis factor;Peroxisome proliferator-activated receptor γ   
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        发布时间：2024-09-23
      • CHEN Chuan-ping,ZHANG Ting-ting,CAO Chen-he,QI Yuan,NIE Liu-wang
        Vol. 13, Issue 1, Pages: 29-31(2007)
        关键词：Grapefruit;polysaccharides;hyperlipidemia;Lipid-decreasing   
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      • CHEN Chuan-ping,ZHANG Ting-ting,CAO Chen-he,QI Yuan,NIE Liu-wang
        Issue 1, Pages: 29-31(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.013
        摘要：Objective:To study the effects of polysaccharides in the grapefruit（GPs）on experimental mice with hyperlipidemia.Method:The water soluble polysaccharides of grapefruit were extracted in hot water,and isolated by ethanol.GPs were purified with Sephadex-50 column chromatography.The hyperlipidemia mice were fed with different doses of GPs to determine the mice serum lipids of control,GPs and model groups after two weeks feeding and to figure out atherosclerosis index（AI）.Result:The molecular weight of GPs were estimated to be（27 000） and（12 000） respectively.Compared with the model group,the concentrations of total cholesterol（TC）,total triglyeride（TG）,low density lipoprotein cholesterol（LDL-C） and AI in serum of GPs groups were decreased significantly（P<0.01）,and the concentration of high density lipoprotein cholesterol（HDL-C）in serum was obviously increased in the meantime（P<0.01）.Conclusion:The results implicate that the GPs have significantly effects in decreasing the blood lipid of hyperlipidemia and in preventing atherosclerosis.  
        关键词：Grapefruit;polysaccharides;hyperlipidemia;Lipid-decreasing   
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        发布时间：2024-09-23
      • ZHEN Han-shen,LIANG Jie,Zhou Fang
        Vol. 13, Issue 1, Pages: 32-34(2007)
        摘要：Objective:To study the anti-hyperglycemic effect of Gymnema sylvestre(Retz.)Schult 95% Ethanolic Extracts(GSEE).Method:Normal mice,alloxan-induced diabetic mice,adrenaline-induced hyperglycemic mice were used.The anti-hyperglycemic action was studied with radio-immunoassay and glucoseoxidase.Result:High dosage of GSEE could decrease fasted blood sugar in normal mice.It could increase serum insulin level,decrease fasted blood sugar in alloxan-induced diabetic mice.It could also decrease fasted blood sugar in adrenaline-induced hyperglycemic mice.It could improve glucose tolerance in normal mice,and the duration was about 1 hour.Conclusion:GSEE has obvious anti-hyperglycemic effect in alloxan-induced diabetic mice and adrenaline-induced hyperglycemic mice.It could improve glucose tolerance in normal mice.Its mechanism is stimulating of insulin secretion from β cells.  
        关键词：Gymnema sylvestre(Retz.)Schult 95% Ethanolic Extracts;alloxan;adrenaline;blood sugar;insulin   
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      • ZHEN Han-shen,LIANG Jie,ZHOU Fang
        Issue 1, Pages: 32-34(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.014
        摘要：Objective:To study the anti-hyperglycemic effect of Gymnema sylvestre（Retz.）Schult 95% Ethanolic Extracts（GSEE）.Method:Normal mice,alloxan-induced diabetic mice,adrenaline-induced hyperglycemic mice were used.The anti-hyperglycemic action was studied with radio-immunoassay and glucoseoxidase.Result:High dosage of GSEE could decrease fasted blood sugar in normal mice.It could increase serum insulin level,decrease fasted blood sugar in alloxan-induced diabetic mice.It could also decrease fasted blood sugar in adrenaline-induced hyperglycemic mice.It could improve glucose tolerance in normal mice,and the duration was about 1 hour.Conclusion:GSEE has obvious anti-hyperglycemic effect in alloxan-induced diabetic mice and adrenaline-induced hyperglycemic mice.It could improve glucose tolerance in normal mice.Its mechanism is stimulating of insulin secretion from β cells.  
        关键词：Gymnema sylvestre（Retz.）Schult 95% Ethanolic Extracts;alloxan;adrenaline;blood sugar;insulin   
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        发布时间：2024-09-23
      • ZHANG Gan-lin1,2,LI Ping1,WANG Xiao-min3,TANG Yong3,LIU Xin1,SHENG Xun1
        Vol. 13, Issue 1, Pages: 35-38(2007)
        摘要：Objective:To study the effect of Gubenyiliu Prescription Ⅱ(Prescription Ⅱ,PⅡ) and its functional groups on A549 lung carcinoma cell's growth and combinative effect with diamminedichloroplatinum(DDP).Methods:According to its functional composition,Prescription Ⅱ was divided into four groups:Reforcing vital energy group(VE)、Promoting qi to activate blood group(QB)、Cultivating kidney and liver group(KL)and Eliminating stagnation and removing lump group(SL).Their aqueous extracts were prepared respectively.MTT assay was used for A549 growth and combinative effect with DDP.Results:Both Prescription Ⅱ and QB group showed depressive effect on A549 growth.The IC50 was 33.21 mg crude drug/mL and 9.55 mg crude drug/mL respectively.Both of them had synergistic action with DDP,the multiple was 1.23 and 1.77,complicating index was 1.05 and 0.83 dccordingly.Conclusion:PⅡ can inhibit A549 grow,and it has synergistic action with DDP to some extent.Among the functional groups,QB group has the similar effects with PⅡ.It indicates that herbs in QB group play the main active effect against tumour and synergetic action in the whole prescription.  
        关键词：Gubenyiliu PrescriptionⅡ;anti-tumour;synergetic action   
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      • ZHANG Gan-lin~
        Issue 1, Pages: 35-38(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.015
        摘要：Objective:To study the effect of Gubenyiliu Prescription Ⅱ（Prescription Ⅱ,PⅡ） and its functional groups on A549 lung carcinoma cell’s growth and combinative effect with diamminedichloroplatinum（DDP）.Methods:According to its functional composition,Prescription Ⅱ was divided into four groups:Reforcing vital energy group（VE）、Promoting qi to activate blood group（QB）、Cultivating kidney and liver group（KL）and Eliminating stagnation and removing lump group（SL）.Their aqueous extracts were prepared respectively.MTT assay was used for A549 growth and combinative effect with DDP.Results:Both Prescription Ⅱ and QB group showed depressive effect on A549 growth.The IC50 was 33.21 mg crude drug/mL and 9.55 mg crude drug/mL respectively.Both of them had synergistic action with DDP,the multiple was 1.23 and 1.77,complicating index was 1.05 and 0.83 dccordingly.Conclusion:PⅡ can inhibit A549 grow,and it has synergistic action with DDP to some extent.Among the functional groups,QB group has the similar effects with PⅡ.It indicates that herbs in QB group play the main active effect against tumour and synergetic action in the whole prescription.  
        关键词：Gubenyiliu PrescriptionⅡ;anti-tumour;synergetic action   
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      • LIU Wei,CHAO Xin-yi,HU Huan-ming,CHEN Hua-yang,YIN Na
        Vol. 13, Issue 1, Pages: 39-40(2007)
        摘要：Objective:To investigate the protective effects of Jin Kui Gastric Drug(JK) on gastric mucosal lesion in rats.Methods:The model of gastric mucosal lesion in rats was induced by aspirin,the indexes of gastric mucosal lesion in experimental groups were observed and determined respectively,the contents of tumour necrosis factor-α(TNF-α),endothelin(ET) and interleukin-1β(IL-1β) in blood serum were measured. Results:The indexes of gastric mucosal lesion after administration of JK was obviously reduced compared with model group.The coutents of TNFα was markedly decreased and the levels of IL-1β was significantly increased. The contents of ET was also reduced,but it showed no difference between JK group and control.Conclusion:JK can protect gastric mucosal lesion in rats and its mechanism may be related to decrease in contents of TNF-α,ET and increase of the IL-1β level.  
        关键词：Jin Kui Gastric Drug;gastric mucosal lesion;TNF-α;IL-1β;ET   
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      • LIU Wei,CHAO Xin-yi,HU Huan-ming,CHEN Hua-yang,YIN Na
        Issue 1, Pages: 39-40(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.016
        摘要：Objective:To investigate the protective effects of Jin Kui Gastric Drug（JK） on gastric mucosal lesion in rats.Methods:The model of gastric mucosal lesion in rats was induced by aspirin,the indexes of gastric mucosal lesion in experimental groups were observed and determined respectively,the contents of tumour necrosis factor-α（TNF-α）,endothelin（ET） and interleukin-1β（IL-1β） in blood serum were measured. Results:The indexes of gastric mucosal lesion after administration of JK was obviously reduced compared with model group.The coutents of TNFα was markedly decreased and the levels of IL-1β was significantly increased. The contents of ET was also reduced,but it showed no difference between JK group and control.Conclusion:JK can protect gastric mucosal lesion in rats and its mechanism may be related to decrease in contents of TNF-α,ET and increase of the IL-1β level.  
        关键词：Jin Kui Gastric Drug;gastric mucosal lesion;TNF-α;IL-1β;ET   
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      • CHEN Xue-gong1,ZHOU Xue-mei1,ZHANG Qiong-yu2,LONG Zi-jiang1,FANG Xiang-ming1,DONG Chang-wu1,WANG Jian-qing1
        Vol. 13, Issue 1, Pages: 41-43(2007)
        摘要：Objective:To evaluate the effects of DongMeiYin(DMY) on advanced glycation end products(AGEs) in the diabetic rats,and investigate the mechanism of preventing and treating early diabetic nephropathy(DN).Methods:The rat early DN model was induced by injection of streptozotocin(STZ).The changes of the blood glucose,uA1b,uα1-MG and AGEs in the renal cortex and the effects of DMY were observed.Results:The blood glucose,uA1b,uα1-MG and AGEs of the model rats were obviously increased.DMY could decrease the parameters effectively.Conclusion:DMY could inhibits the AGEs and reduce the ua1b,uα1-MG,blood glucose.  
        关键词：diabetic nephropathy;DongMeiYin;Advanced glycation end products   
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      • CHEN Xue-gong~
        Issue 1, Pages: 41-43(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.017
        摘要：Objective:To evaluate the effects of DongMeiYin（DMY） on advanced glycation end products（AGEs） in the diabetic rats,and investigate the mechanism of preventing and treating early diabetic nephropathy（DN）.Methods:The rat early DN model was induced by injection of streptozotocin（STZ）.The changes of the blood glucose,uA1b,uα1-MG and AGEs in the renal cortex and the effects of DMY were observed.Results:The blood glucose,uA1b,uα1-MG and AGEs of the model rats were obviously increased.DMY could decrease the parameters effectively.Conclusion:DMY could inhibits the AGEs and reduce the ua1b,uα1-MG,blood glucose.  
        关键词：diabetic nephropathy;DongMeiYin;Advanced glycation end products   
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      • ZHANG Shuo-feng1,SHEN Xin2,WU Jin-ying1,JIA Zhan-hong1
        Vol. 13, Issue 1, Pages: 44-45(2007)
        摘要：Objective:To observe the effects of Yangxueqingnao Granule(YXQNG) on analgesia and cerebral blood flow in mice.Methods:Microcirculatory impairment of mice was induced by intravenous injection of high molecular dextran(HMD).The possible improvement of cerebral blood flow by YXQNG was investigated.The other animal model was pain in mice induced by pressure and acetic acid.Changes produced by YXQNG in mechanical withdrawal threshold and body twisting were evaluated.Results:YXQNG(1,2 g/kg) could effectively increase the value of mechanical withdrawal threshold in mice.YXQNG(1,2 and 4 g/kg) could effectively decrease the number of body twisting of mouse and increase cerebral blood flow in mice with HMD injection.Conclusion:YXQNG may have the effects of relieving pain and increasing cerebral blood flow.  
        关键词：Yangxueqingnao Granule;Microcirculatory impairment;analgesia   
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      • ZHANG Shuo-feng~
        Issue 1, Pages: 44-45(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.018
        摘要：Objective:To observe the effects of Yangxueqingnao Granule（YXQNG） on analgesia and cerebral blood flow in mice.Methods:Microcirculatory impairment of mice was induced by intravenous injection of high molecular dextran（HMD）.The possible improvement of cerebral blood flow by YXQNG was investigated.The other animal model was pain in mice induced by pressure and acetic acid.Changes produced by YXQNG in mechanical withdrawal threshold and body twisting were evaluated.Results:YXQNG（1,2 g/kg） could effectively increase the value of mechanical withdrawal threshold in mice.YXQNG（1,2 and 4 g/kg） could effectively decrease the number of body twisting of mouse and increase cerebral blood flow in mice with HMD injection.Conclusion:YXQNG may have the effects of relieving pain and increasing cerebral blood flow.  
        关键词：Yangxueqingnao Granule;Microcirculatory impairment;analgesia   
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      • Issue 1, Pages: 46-48(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.019
        摘要：目的:观察以益气养血法组方药物对正常及便秘模型小鼠胃肠动力的影响。方法:用酚红法检测胃排空功能;墨水推进法检测小肠、结肠运动功能;用NADPH-黄递酶（NDP）组化技术和分层铺片方法检测结肠肌间神经丛一氧化氮合酶（NOS）阳性神经元的灰度值。用复方地芬诺酯制备便秘动物模型。结果:益气养血法组方药物3个剂量组的小鼠胃排空率,小肠和结肠推进率与正常对照组相比都有显著性差异（P<0.0010.05）。便秘状态下3个剂量组的小鼠胃排空率,结肠推进率和结肠肌间神经丛NOS阳性神经元的灰度值与模型组相比都有显著性差异（P<0.0010.05）。结论:以益气养血法组方药物对小鼠胃肠动力具有促进作用,可调节便秘小鼠结肠肌间神经丛NOS阳性神经元的兴奋性。  
        关键词：胃肠动力;肠肌间神经丛;一氧化氮合酶;便秘   
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      • REN Ying,SONG Chong-shun,YIN Jun-xiang,SUN Hai-yun,GUO Jing,DAI Xin,YANG Jin-duo,CUI Jie-feng,TAN Wei
        Vol. 13, Issue 1, Pages: 49-51(2007)
        摘要：Objective:To explore the protective effect of Jiaweibiejiajian Pill(JWBJ) on hepatic fibrosis of rats induced by Carbon Tetrachloride(CCl4).Method:Rats model with hepatic fibrosis was induced by the multiple subcutaneous injections of 40% CCl4,3 mL/kg,twice a week for 6 weeks.When hepatic fibrosis was verified to come into being by pathological examination,the rats were divided into different groups.JWBJ was administrated orally for 8 weeks.Then all rats were sacrificed and their liver were taken out and the pathological sections were stained by HE and Masson.The pathological change,the content of hepatic collagen and hepatic plastin of lives were observed.Result:Compared with model group rats,the number of lipoid vacuolation in liver tissue,collagen fibers deposition,Pseudolobuli and fibroblast hyperplasia,the degree of inflammatory cell filtration,degeneration and necrosis in liver tissue were decreased and alleviated obviously in rats of mediate-dose group and high-dose group of JWBJ.Conclusion:JWBJ showed the protective function for hepatic fibrosis and could alleviate the degree of degeneration and necrosis induced by CCl4.  
        关键词：Jiaweibiejiajian pill;hepatic fibrosis;CCl4   
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      • REN Ying,SONG Chong-shun,YIN Jun-xiang,SUN Hai-yun,GUO Jing,DAI Xin,YANG Jin-duo,CUI Jie-feng,TAN Wei
        Issue 1, Pages: 49-51(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.020
        摘要：Objective:To explore the protective effect of Jiaweibiejiajian Pill（JWBJ） on hepatic fibrosis of rats induced by Carbon Tetrachloride（CCl4）.Method:Rats model with hepatic fibrosis was induced by the multiple subcutaneous injections of 40% CCl4,3 mL/kg,twice a week for 6 weeks.When hepatic fibrosis was verified to come into being by pathological examination,the rats were divided into different groups.JWBJ was administrated orally for 8 weeks.Then all rats were sacrificed and their liver were taken out and the pathological sections were stained by HE and Masson.The pathological change,the content of hepatic collagen and hepatic plastin of lives were observed.Result:Compared with model group rats,the number of lipoid vacuolation in liver tissue,collagen fibers deposition,Pseudolobuli and fibroblast hyperplasia,the degree of inflammatory cell filtration,degeneration and necrosis in liver tissue were decreased and alleviated obviously in rats of mediate-dose group and high-dose group of JWBJ.Conclusion:JWBJ showed the protective function for hepatic fibrosis and could alleviate the degree of degeneration and necrosis induced by CCl4.  
        关键词：Jiaweibiejiajian pill;hepatic fibrosis;CCl4   
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      • HAN Tao1,LAN Yong-mei2,AN Dong-qing3
        Vol. 13, Issue 1, Pages: 52-54(2007)
        摘要：Objective:To study the effects of Shukang Pingchuan Capsule(SKPCC) on IFN-γ,IL-4,IL-5 and IFN-γ/IL-4 in Asthmatic Rats.Methods:Rats are divided into 6 groups:normal control group,asthmatic model group,SKPCC with large,medium,and small dose group,and hormone(dexamethasone) group.Asthmatic model was established by sensitizing and challenging rats with OVA.The level of IFN-γ,IL-4 and IL-5 and the IFN-γ/IL-4 ratio in BALF was measured by ELISA.Results:The IFN-γ level was higher(P<0.05),the IL-4 and IL-5 level significantly lower(P<0.01 orc P<0.05) in large and medium SKPCC treatment group compared with asthmatic model group.The IFN-γ/IL-4 ratio in SKPCC treatment group was significantly higher than that in asthmatic model group(P<0.01).Conclusion:SKPCC can adjust Th1/Th2 in asthmatic rats,inhibite the inflammation in trachea and lower its high reaction,which may be one of the mechanisms of SKPCC in treating asthma.  
        关键词：Shukang Pingchuan Capsule;asthma;IFN-γ;IL-4;IL-5   
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      • HAN Tao~
        Issue 1, Pages: 52-54(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.021
        摘要：Objective:To study the effects of Shukang Pingchuan Capsule（SKPCC） on IFN-γ,IL-4,IL-5 and IFN-γ/IL-4 in Asthmatic Rats.Methods:Rats are divided into 6 groups:normal control group,asthmatic model group,SKPCC with large,medium,and small dose group,and hormone（dexamethasone） group.Asthmatic model was established by sensitizing and challenging rats with OVA.The level of IFN-γ,IL-4 and IL-5 and the IFN-γ/IL-4 ratio in BALF was measured by ELISA.Results:The IFN-γ level was higher（P<0.05）,the IL-4 and IL-5 level significantly lower（P<0.01 or P<0.05） in large and medium SKPCC treatment group compared with asthmatic model group.The IFN-γ/IL-4 ratio in SKPCC treatment group was significantly higher than that in asthmatic model group（P<0.01）.Conclusion:SKPCC can adjust Th1/Th2 in asthmatic rats,inhibite the inflammation in trachea and lower its high reaction,which may be one of the mechanisms of SKPCC in treating asthma.  
        关键词：Shukang Pingchuan Capsule;asthma;IFN-γ;IL-4;IL-5   
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      • Issue 1, Pages: 55-57(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.022
        摘要：目的:研究开心散及其各成分（人参、远志、石菖蒲和茯苓）的体外抗氧化作用,并对其有效成分进行研究,寻找最佳配比。方法:研究开心散的体外抗脂质过氧化（LPO）作用,再选用均匀设计和正交设计,研究开心散体外抗LPO作用的配伍规律,找出其中的有效成分,并对其有效成分进行研究。结果:选用320、160、40mg/mL三个剂量组,通过与阴性对照组比较,发现开心散具有降LPO的作用,其中高剂量组和低剂量组抗LPO作用更明显,但3个剂量组与维生素E组比较后提示开心散抗LPO的作用不如维生素E;应用U10*（108）设计对开心散进行研究,发现四个组成成分中远志具有抗LPO的作用,而茯苓的作用则正好相反,其它成分作用不显著,而通过L12（211）设计验证,仅远志具有较明显的抗LPO作用;另对远志与维生素E比较后发现,0.5g/mL远志与10mg/L维生素E的抗LPO作用相当。结论:开心散具有降LPO的作用,其中起作用的为远志。  
        关键词：开心散;体外抗脂质过氧化作用;均匀设计;正交设计;远志   
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      • ZHAO Long-po,XU Zheng,ZHANG Wen,SUN Hui-chen,TANG Fei
        Vol. 13, Issue 1, Pages: 58-61(2007)
        摘要：Objective:To establish in vitro culture of rat Sertoli cells and to investigate the effects of Chinese herbal medicines to their functions and the mechanism.Methods:Sertoli cells aged 18～22 days were separated and cultured from rat testis;the effect of Fructus Lycii,Radix Astragali and the combined administration of the two medicines on the proliferation of Sertoli cells in vitro were detected by means of MTT assay;the effect of Fructus Lycii,Radix Astragali and the combined administration of the two medicines on the level of INHβB in Sertoli cells in vitro were investigated in both normal environment and peroxide-damaging environment by means of immunohistochemistry.Results:The proliferation of Sertoli cells in vitro was promoted when treated with Fructus Lycii,Radix Astragali and the combined administration of the two medicines;Sertoli cells INHβB was up regulated by Fructus Lycii,Radix Astragali and the combined administration of the two medicines in vitro;Fructus Lycii and Radix Astragali were found to have protective effect on synthesis of INHβB in Sertoli cells with injury induced by peroxide(H2O2).Conclusion:The function of promotion and protection were found when Sertoli cells cultured in vitro treated with Fructus Lycii,Radix Astragali and the combined administration of the two medicines.  
        关键词：Fructus Lycii;Radix Astragali;Sertoli cells;Inhibin   
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      • ZHAO Long-po,XU Zheng,ZHANG Wen,SUN Hui-chen~,TANG Fei
        Issue 1, Pages: 58-61(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.023
        摘要：Objective:To establish in vitro culture of rat Sertoli cells and to investigate the effects of Chinese herbal medicines to their functions and the mechanism.Methods:Sertoli cells aged 18～22 days were separated and cultured from rat testis;the effect of Fructus Lycii,Radix Astragali and the combined administration of the two medicines on the proliferation of Sertoli cells in vitro were detected by means of MTT assay;the effect of Fructus Lycii,Radix Astragali and the combined administration of the two medicines on the level of INHβB in Sertoli cells in vitro were investigated in both normal environment and peroxide-damaging environment by means of immunohistochemistry.Results:The proliferation of Sertoli cells in vitro was promoted when treated with Fructus Lycii,Radix Astragali and the combined administration of the two medicines;Sertoli cells INHβB was up regulated by Fructus Lycii,Radix Astragali and the combined administration of the two medicines in vitro;Fructus Lycii and Radix Astragali were found to have protective effect on synthesis of INHβB in Sertoli cells with injury induced by peroxide（H2O2）.Conclusion:The function of promotion and protection were found when Sertoli cells cultured in vitro treated with Fructus Lycii,Radix Astragali and the combined administration of the two medicines.  
        关键词：Fructus Lycii;Radix Astragali;Sertoli cells;Inhibin   
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      • Issue 1, Pages: 62-63(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.024
        摘要：目的:研究中药复方“五子衍宗丸”对糖尿病性白内障小鼠氧化损伤的影响。方法:小鼠60只随机分为5组,空白组（A组）,模型组（B组）,维生素C治疗组（C组）,中药预防组（D组）,中药治疗组（E组）。B、C、D、E 4组按20 mL/kg腹腔注射10%D-半乳糖溶液20 d造模,空白组注射相应剂量的生理盐水,D组以1.875 g/kg五子衍宗丸水溶剂灌胃,1次/d。造模结束后,C组以维生素C水溶液灌胃治疗,E组用五子衍宗丸水溶剂灌胃治疗,测定晶状体和血清中MDA含量,SOD和GSH-Px的活性。结果:五子衍宗丸可提高红细胞超氧化物歧化酶（SOD）,谷胱甘肽过氧化物酶（GSH-PX）的活性（P<0.05或P<0.01）,降低丙二醛（MDA）的含量（P<0.01）,能保持小鼠透明晶状体的百分率达40%。结论:五子衍宗丸是通过对抗D-半乳糖所致的氧化损伤,延缓糖尿病性白内障病变发展的进程。  
        关键词：白内障;糖尿病;自由基;五子衍宗丸   
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      • WANG Xiao-juan,LEI Ying,GUO Jian-sheng,GUO Xuan,HU Fang
        Vol. 13, Issue 1, Pages: 64-66(2007)
        摘要：Objective:To observe the effect of Shen Shuai KeLi on endothelin(ET) and nitrogen monoxide(NO)of chronic renal failure(CRF).Method:60 patients with CRF were randomly divided into two groups.30 patients in treatment group were treated with ShenShuai KeLi;the other 30 patients in control group were treated with oxyamyli tectus aldehydum.The blood urea nitrogen(BUN),serum creatinins(Scr),NO,ET and creatinine clearance rate(Ccr) were detected in both groups.Results:Compared with those in control group,the BUN,Cr,ET in treatment group decreased significantly(P<0.01),and the NO,Ccr in treatment group increased significantly(P<0.01).Conclusion:ShenShuai KeLi can decrease the BUN,ET,and Scr and increase NO and Ccr in patients with chronic renal failure,so it may delay the progress of chronic renal failure.  
        关键词：ShenShuai KeLi;chronic renal failure;endothelin;Nitrogen monoxide   
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      • WANG Xiao-juan,LEI Ying,GUO Jian-sheng,GUO Xuan,HU Fang
        Issue 1, Pages: 64-66(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.025
        摘要：Objective:To observe the effect of Shen Shuai KeLi on endothelin（ET） and nitrogen monoxide（NO）of chronic renal failure（CRF）.Method:60 patients with CRF were randomly divided into two groups.30 patients in treatment group were treated with ShenShuai KeLi;the other 30 patients in control group were treated with oxyamyli tectus aldehydum.The blood urea nitrogen（BUN）,serum creatinins（Scr）,NO,ET and creatinine clearance rate（Ccr） were detected in both groups.Results:Compared with those in control group,the BUN,Cr,ET in treatment group decreased significantly（P<0.01）,and the NO,Ccr in treatment group increased significantly（P<0.01）.Conclusion:ShenShuai KeLi can decrease the BUN,ET,and Scr and increase NO and Ccr in patients with chronic renal failure,so it may delay the progress of chronic renal failure.  
        关键词：ShenShuai KeLi;chronic renal failure;endothelin;Nitrogen monoxide   
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        发布时间：2024-09-23
      • Issue 1, Pages: 67-68(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.026
        摘要：目的:探讨早产儿脐血及母血β-内啡肽（-βEP）浓度与胎龄、体重的关系。方法:选择早产产妇60例,在第一产程采母亲静脉血3 mL,脐血3 mL,早产儿30例分别于生后12 h、24 h3、d、5 d采血,用放射免疫法测定血浆-βEP浓度。结果:胎龄≤32周、体重≤1 500 g组早产儿脐血-βEP浓度高于胎龄>32周、体重>1 500 g组,有非常显著性差异（P<0.01）;胎龄≤32周、体重≤1 500 g组母血-βEP浓度高于胎龄>32周、体重>1 500 g组,有显著性差异（P<0.05）;早产儿出生后不同时间血浆-βEP水平不全相同;早产儿脐血-βEP水平高于足月儿（P<0.01）,有非常显著性差异;早产儿母血-βEP水平高于足月儿母血组（P<0.01）,有非常显著性差异。结论:早产儿胎龄越小,体重越低,脐血及母血-βEP浓度越高,生后5 d内-βEP浓度高于足月儿,与早产儿原发性呼吸暂停的发生呈负相关,为出生后立即应用纳络酮预防原发性呼吸暂停提供了试验依据。  
        关键词：早产;脐血;原发性呼吸暂停;β-内啡肽   
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      • YANG Yuan-xiao,LI Chang-yu
        Vol. 13, Issue 1, Pages: 69-72(2007)
        摘要：objective: To discuss the relation between proteomics and TCM syndrome.Methods: By broadly reference to literatures,to make an analysis on the current research status of TCM syndrome and the feasibility of the research aimed at finding out the essential of TCM syndrome by taking advantage of proteomics.Results: Although many achievements have been made in respect of TCM syndrome,but the essential has not been revealed so far;the study of TCM syndrome by proteomics is based on certain theories and practice.Conclusion:The wild use of proteomics is surely going to bring a turning point for studying of TCM syndrome,which contributes to the final discovery of mechanism of diseases and vital activities.  
        关键词：syndrome;essence;proteomics   
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      • YANG Yuan-xiao,LI Chang-yu
        Issue 1, Pages: 69-72(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.027
        摘要：objective: To discuss the relation between proteomics and TCM syndrome.Methods: By broadly reference to literatures,to make an analysis on the current research status of TCM syndrome and the feasibility of the research aimed at finding out the essential of TCM syndrome by taking advantage of proteomics.Results: Although many achievements have been made in respect of TCM syndrome,but the essential has not been revealed so far;the study of TCM syndrome by proteomics is based on certain theories and practice.Conclusion:The wild use of proteomics is surely going to bring a turning point for studying of TCM syndrome,which contributes to the final discovery of mechanism of diseases and vital activities.  
        关键词：syndrome;essence;proteomics   
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      • Vol. 0, Issue 1, Pages: 73(2007)
          
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      • Vol. 0, Issue 1, Pages: 74(2007) DOI: 10.13422/j.cnki.syfjx.2007.01.001
          
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