最新刊期
卷 21 ， 期 15 ， 2015
- 摘要：Objective: Taking drug dissolution and absorption simulating system (DDASS) to evaluate in vitro release and trans-membrane characteristics of Leigongteng bilayer tablets,and study in vivo and in vitro correlation between DDASS and the preparation in Beagle dogs. Method: With wilfordine as the index component,release characteristics of Leigongteng tablets and Leigongteng bilayer tablets were evaluated by the rotating basket method,in order to study in vitro continuous,dynamic,real-time release and trans-membrane characteristics in DDASS model as well as pharmacokinetic characteristics of these two preparations in Beagle dogs and their corresponding in vivo and in vitro correlation. Result: Release processes of wilfordine in Leigongteng bilayer tablets in the rotating basket method and DDASS conformed to the first-order kinetic equation and the skeleton corrosion mechanism.Compared with Leigongteng tablets,Leigongteng bilayer tablets showed obvious sustained release of wilfordine in Beagle dogs with the relative bioavailability of 174.88%.In DDASS,release/trans-membrane characteristics was significantly correlated to dog absorption characteristics,which was superior to the correlation between dissolution of the rotating basket method and Beagle dog absorption characteristics. Conclusion: DDASS model can effectively evaluate in vitro release kinetics and in vivo absorption kinetics of wilfordine in Leigongteng bilayer tablets.关键词：sustained release preparation;Leigongteng bilayer tablets;drug dissolution/absorption simulating system;drug release characteristic;in vitro-in vivo correlation;wilfordine
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Bupleuri Radix with turtle blood. Method: With composite score of saikosaponin a,c,d and ethanol soluble extractives as index,an orthogonal test was adopted to investigate influence of processing time,turtle blood amount and processing temperature on processing technology of Bupleuri Radix processed with turtle blood.HPLC was adopted to determine contents of saikosaponin a,c,d and eluted in gradient with mobile phase of acetonitrile-water,detection wavelength was at 210 nm. Result: Stir-frying time had a significant influence on processing technology.According to optimum processing technology,processing temperature was set at 150 ℃,processing time was 10 min,the amount of turtle blood was 0.05 mL · g-1.Mass fractions of ethanol soluble extractives and saikosaponin a,c,d were 12.42%,0.85%,0.23%,0.90%,respectively. Conclusion: This optimized processing technology is reasonable,reliable and highly reproducible,it can provide a reference for processing of Bupleuri Radix processed with turtle blood.关键词：Bupleuri Radix processed with turtle blood;saikosaponin A;saikosaponin c;saikosaponin D;ethanol soluble extractives
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发布时间：2024-01-04 - 摘要：Objective: To conduct a qualitative and quantitative analysis on volatile oil from raw,bran-fried,honey bran-fried and honey chaff-fried Aurantii Fructus Immaturus from Jiangxi by GC-MS. Method: Volatile oil was extracted from Aurantii Fructus Immaturus by steam distillation method.Volatile oil in different processed products of Aurantii Fructus Immaturus were determined by GC-MS under mass spectrometer conditions of 70 eV,ion source temperature at 230 ℃,accelerating voltage of 34.6 V,multiplier voltage of 1 388 V,quadrupole rod temperature at 150 ℃,scanning frequency of 4.45 times/s.Peak area normalization method was used to determine the relative mass fraction of each component. Result: Altogether 163 components were separated and identified from raw,bran-fried,honey bran-fried and honey chaff-fried Aurantii Fructus Immaturus.A wider varieties of new compound were founded from processed products of Aurantii Fructus Immaturus by excipients,with the most varieties of volatile oils in bran-fried Aurantii Fructus Immaturus.Bran-fried,honey chaff-fried,honey bran-fried and raw Aurantii Fructus Immaturus contained 99,87,81,79 new compounds,including 42 types of ingredients in total.Compared with raw Aurantii Fructus Immaturus,bran-fried products had 52 more types of new compounds;honey bran-fried products had 26 more types of new compounds;and honey chaff-fried products had 28 more types of new compounds. Conclusion: GC-MS is suitable for analyzing chemical components in volatile oil from Aurantii Fructus Immaturus,which can provide an experimental reference for quality evaluation and processing mechanism studies of processed procucts of Aurantii Fructus Immaturus.关键词：Aurantii Fructus Immaturus from Jiangxi;processed products;volatile oil;limonene;γ-terpinene;α-terpineol
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of honey bran-fried Atractylodis Macrocephalae Rhizoma,in order to provide experimental basis for inheriting Jianchangbang processing technology. Method: Central composite design-response surface methodology was used to optimize processing technology of honey bran-fried Atractylodis Macrocephalae Rhizoma and make a predictive analysis with overall desirability (OD) of contents of atractylenolide I,III and water extracts as the dependent variable,honey bran consumption,frying time and frying temperature as independent variables. Result: Optimum processing technology was 55.8% of honey bran fried for 3.4 min at 261 ℃.Mass fractions of water extracts and atractylenolide I,III were 71.5%, 0.028%, 0.069% with ODs of 0.74,0.76,0.72,respectively.Deviation between the predicted value and the actual value of OD was less than 1.4%. Conclusion: Central composite design-response surface methodology for optimizing processing technology of honey bran-fried Atractylodis Macrocephalae Rhizoma is stable and highly predictable,it can provide a reference for standardizing local decoction pieces processing technologies and their quality.关键词：atractylenolide Ι;Atractylenolide Ⅲ;water extract;overall desirability;Atractylodis Macrocephalae Rhizoma
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation technology of Zingiberis Rhizoma,in order to provide a reference for preparing Zingiberis Rhizoma liquor. Method: With composite score of contents of 6-gingerol and volatile oil as evaluation index,effects of slice specifications,drying temperature and drying time on preparation technology of Zingiberis Rhizoma was studied by orthogonal test. Result: Optimal preparation technology was to slice for 0.3 cm,set oven temperature at 55 ℃ and dry for 18 h.Mass fractions of 6-gingerol and volatile oil were 0.914% and 1.273%,respectively. Conclusion: This optimized preparation process is feasible and so can be used as process of pharmaceutical product for Zingiberis Rhizoma liquor.关键词：Zingiberis Rhizoma;6-gingerols;volatile oil;slice specifications
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发布时间：2024-01-04 - 摘要：Objective: To prepare tannin lappaconitine compound,investigate its physicochemical properties and make an evaluation. Method: Tannin was used to deposit alkaloids for preparing tannin lappaconitine compound,which was identified by differential scanning calorimetry (DSC),ultraviolet spectrum (UV) and Fourier transform infrared spectroscopy (FT-IR).The molar ratio between lappaconitine and tannin was determined by UV.The compound's sedimentation capacity in equal amount of proteins was determined in simulated artificial gastric juice,small intestine and colon fluid environments.On the basis of the compound's blood coagulation capacity,surface wound model was established to determine the compound's healing capability in Wistar rats. Result: Lappaconitine and tannin formed a new compound with the molar ratio of 1: 1.In simulated artificial gastric juice,small intestine and colon fluid environments,tannin lappaconitine compound's sedimentation capacity with equal amount of proteins were 0.63,0.46,0.92 mg,respectively.In terms of the wound healing capacity,the compound group was significantly higher than the control group and even superior to the positive tannin group. Conclusion: Lappaconitine and tannin form a type of new compound through the hydrogen bonding interaction.This compound can achieve blood coagulation effect through sedimentation of proteins in blood,and promote wound healing,hemostasis,anti-inflammatory analgesic effects by increasing its biological value.关键词：tannin;lappaconitine;protein sedimentation;surface wound
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of different processing methods on stilbene glycoside and anthraquinones in Polygoni Multiflori Radix (PMR) decoction, in order to provide the basis for establishing suitable processing methods for PMR decoction. Method: Different slice thicknesses, drying methods and temperatures were adopted to process PMR samples. TLC method was adopted to qualitatively analyze PMR. Stilbene glycoside and anthraquinones were determined by HPLC to define the effect of different processing methods on the quality of PMR decoction. Result: TLC showed obvious spot characteristics. Different drying temperatures and drying methods had a greater impact on the content of effective components in PMR. Samples dried in the sun had the highest content according to the comprehensive analysis. Different slice thicknesses had a certain effect on the content of effective components;6 mm slices contained more than 3 mm slices. Conclusion: Different processing methods had a greater impact on the content of effective components in PMR. In this paper, the authors provided a certain basis to improve the quality control of PMR, which is significant to ensure PMR's quality and clinical efficiency.关键词：Polygoni Multiflori Radix;processing method;quality control;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method to identify growing years of Paridis Rhizoma,in order to provide basis for quality control of this plant. Method: A total of 68 epidermises and xylems of taproot samples with seven different growing years of Paridis Rhizoma from the same origin was analyzed by Fourier-transform infrared (FTIR) spectroscopy.The second derivative spectra and fourth derivative spectra of these tested samples were obtained by Omnic8.0 software.With the infrared spectra,the second derivative spectra and the fourth derivative spectra of epidermises and xylems of taproot samples ranging between 1 800 and 900 cm-1 as the sample characteristics,the discriminant model of growing years of Paridis Rhizoma was established by Mahalanbis distance gradual discriminant analysis method on the basis of Fisher linear discriminant criterion,in order to identify and compare these tested samples with unknown growing years. Result: The fourth derivative spectra had a better discrimination effect than other types of infrared spectra,while epidermises samples had a better discrimination effect than xylems samples.Moreover,the discriminant analysis model was established based on the fourth derivative spectra of epidermises to identify growing years of Paridis Rhizoma,with the return discriminant accuracy of training samples of 100%,the predicted accuracy of tested samples of 78.6% and a total accuracy of 95.6%. Conclusion: FTIR combined with gradual discriminant analysis can be utilized to identify growing years of Paridis Rhizoma and provide a new method for prediction of growing years of Paridis Rhizoma in the production practice.关键词：infrared spectroscopy;discriminant analysis;Paridis Rhizoma;growing years;xylem;epidermises
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the quality of different batches of stir-baked Leonuri Fructus and make a comparison by using TOPSIS and grey correlation analysis. Method: The percentage composition of total ash, acid-insoluble ash, extractum and stachydrine hydrochloride in different batches of stir-baked Leonuri Fructus were determined. The analytic hierarchy process was used to determined the four index weights 0.077, 0.077, 0.385, 0.461.MATLAB programming was adopted to build the grey correlation quality evaluation model for stir-baked Leonuri Fructus from different producing areas and compare the TOPSIS results. Result: According to the grey correlation analysis on components in the different batches of stir-baked Leonuri Fructus, Leonuri Fructus with the batch of “Guangdong 20121117” was the most highly correlated with the optimal reference sequence (0.561 685 996) and so the best in quality;Leonuri Fructus with the batch of “Henan 20130401” was the most lowly correlated with the optimal reference sequence (0.402 108 175) and so the worst in quality. The results are basically identical with that of TOPSIS, indicating a good repeatability and stability. Conclusion: The grey recognition model is applicable for the comprehensive quality evaluation of multiple-component and multi-target samples of traditional Chinese medicines, with a good promotion and application prospect.关键词：stir-baked Leonuri Fructus;grey correlation analysis;TOPSIS;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of lactic acid content in subcutaneous micro-dialysis fluid of hepatoma-bearing mice by micro-dialysis combined with HPLC. Method: Micro-dialysis system was adopted to collect subcutaneous micro-dialysis fluid of normal mice and tumor-burdened mice for determining the content of lactic acid in subcutaneous micro-dialysis fluid by HPLC.The ZORBAX SB-Aq column (4.6 mm×150 mm,5 μm) was used with the mobile phase of methanol-0.1% phosphoric acid solution (5: 95) at a flow rate of 1.0 mL · min-1,the detection wavelength was 210 nm, and the column temperature was set at 30 ℃. Result: The linearity ranged between 20.39-611.6 mg · L-1 with the average recovery rate of 97.41% and RSD of 4.1%. Conclusion: This method is simple,reliable,rapid and highly sensitive,it can accurately detect lactic acid content in dialyzate and provide a new way to detect lactic acid.关键词：lactic acid;micro-dialysis;HPLC;H22-hepatoma bearing mice
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC-DAD fingerprint to conduct a quantitative analysis on the 14 index ingredients of Gegen Tang (GGD) and provide a method for the quality control for the water extracts from GGD. Method: The AgelaVenusil MP-C18was adopted and eluted in gradient with acetonitrile and water (consisting 0.1% formic acid) at 30 ℃. The flow rate was 1.0 mL · min-1, and the detection wavelength was set at 207, 230, 254 and 275 nm. Result: Totally 38 common peaks were marked and 10 were identified in this fingerprint, with a good similarity (≥0.978) and less difference among the 11 batches. Specifically, 14 index components in the 11 batches of GGD feature even and stable content, stable sample preparation method and controllable quality. Conclusion: The combination of fingerprint and quantitative analysis can roundly reflected the quality of GGD and can be taken as an effective method for controlling the quality of the water extracts from GGD.关键词：Gegen Tang;fingerprint;HPLC;quality evaluation;primary dysmenorrhea
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发布时间：2024-01-04 - 摘要：Objective: To establish a method to determine the salicylaldehyde in Filipendula ulmari by gas chromatography (GC). Method: By using GC, RTX-1701 column (0.25 mm×30 m, 0.25 μm) was analyzed with FID detector. The detector and sample inlet temperature were set at 250 ℃. The temperature programming began at 70 ℃ and increased to 105 ℃ at the rate of 1 ℃ · min-1 with in 10 min and then to 250 ℃ at the rate of 15 ℃ within 15 min. Nitrogen was the carrier gas and flew at the rate of 1 mL · min-1. Split sampling was adopted, with the split ratio of 80: 1 and sample size of 1 μL. Result: The linear ranges for salicylaldehyde were 0.191 7-1.917 0 g · L-1(r=0.999 9), with the average recovery of 100.12%(RSD 2.5%). Conclusion: The method is simple, accurate and highly reliable and so can be used for the determination of salicylaldehyde in F. ulmari.关键词：Filipendula ulmari;salicylaldehyde;GC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method to evaluate the quality of Tinosporae Radix. Method: By using the HPLC method, the Agilent ZORBXSB-C18 column (4.6 mm×150 mm, 5 μm) was adopted and eluted in gradient with the mobile phase of acetonitrile-water (0.5% phosphoricacid). The flow rate was 1 mL · min-1, the detection wave length was 280 nm, and the injection volume was 10 μL. HPLC fingerprints of different varieties of Tinosporae Radix from different producing areas were established to determine the content of jatrorrhizine hydrochloride and martin hydrochloride in different varieties. Result: There were significant differences between different varieties of Tinosporae Radix in their HPLC fingerprints and contents of jatrorrhizine hydrochloride and martin hydrochloride. The fingerprints for alkaloid constituents in Tinosporae Radix showed eight common peaks, with differences in the alkaloid components in Tinosporae Radix from different producing areas. The index R indicating the content may not high in fingerprints with a high similarity.The index R may not low in fingerprints with a low similarity. The index R changed with the index component content determination results. Conclusion: The index component content determination method and fingerprint established in this study was highly precision, stable and repeatable, and can be used to represent the differences in the quality of Tinosporae Radix and provide a reference basis for the quality study on Tinosporae Radix.关键词：Tinosporae Radix;jatrorrhizine hydrochloride;martin hydrochloride;fingerprint;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for analysis of Penthorum chinense flos by HPLC fingerprint and compare the fingerprints of caulis, folium and flos, and to seek ways to the quality assessment and quality control of different parts of P. chinense. Method: The analysis was performed on an ODS column with acetonitrile-0.1% formic acid as mobile phase in gradient elution mode. The flow rate was 0.8 mL · min-1,the column temperature was 30 ℃ and the injection volume was 10 μL with the detection wavelength of 280 nm. Result: Fifteen common peaks were separated on HPLC fingerprint of the flos of P. chinense, including the fifth peak of rutin peak, the ninth peak of quercetin peaks. The similarities of 10 batches of samples were in the range of 0.925-0.960.When the flos of P. chinense was compared with caulis and folium, we found that flos has the most common peaks and caulis has the least. Besides, significant variability was found among the caulis, folium and flos, the contents of marker peak in the flos were more than that in the caulis and folium. Conclusion: The method showed good accuracy, validity and reproducibility characteristics and provided information for the quality assessment and quality control of different parts of P. chinense. To ensure the quality of Chinese medicine, it is recommended that P. chinense should be harvested during florescence.关键词：Penthorum chinense;flos;caulis;folium;HPLC fingerprint
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发布时间：2024-01-04 - 摘要：Objective: Due to the difficulties of dealing with traditional Chinese medicine quality controlling, systematic quantified fingerprint method (SQFM) and the principal component analysis (PCA) were performed to evaluate the quality of Guchang Zhixie (GCZX) pill and powders. Method: The pill and powders were determined by high performance liquid chromatography with diode array detector (HPLC-DAD). Five absorption wavelengths (254, 345, 230, 240,280 nm) were applied to reflect the characterization of six main components from GCZX pill and powders. The 12 batches analytes were processed by SQFM and PCA approaches and berberine hydrochloride was used as reference peak. Result: Refer to berberine hydrochloride peak, 21 common peaks were obtained. According to the results of the SQFM and PCA analysis, qualities of all batches analytes were qualified, except the results under 230 nm. Compared to GCZX powders` fingerprint, a distinct distance from the pills could be investigated on PCA scores plot, meanwhile, 9 component peaks reduced after prepared and 2 peaks disappeared around 13.05 min. Also, validation of the method has been performed. Conclusion: SQFM and PCA methods were successfully tested in GCZX pill and powders fingerprint studies. Results from assay validations showed that these two methods are selective, sensitive and robust. The developed methods were applied to Chinese traditional medicine evaluation.关键词：Guchang Zhixie pill;intermediate;PCA;fingerprint;chromatography
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发布时间：2024-01-04 - 摘要：Objective: A method for determination of Pb, As, Cd, Hg, Cu in Reduning injection by ICP-MS was established. Method: The five elements were detected simultaneously after the samples digested by microwave digestion.Internal standard method was adopted to reduce matrix effect and other interference effects. Result: The detectionlimit was 0.009-0.113 μg · L-1. It showed a good linearity in the range 0-20 μg · L-1 (Pb),0-20 μg · L-1 (As),0-10 μg · L-1 (Cd),0-200 μg · L-1 (Cu),0-5 μg · L-1(Hg);linear relationship is good as r≥0.999.The precision was<1.9%,and recoveries of samples were of the range 97.8%-110.9%.The contents of 5 elements in 20 batches of samples were in In accordance with the provisions. Conclusion: This method is simple,fast,high sensitivity,which can meet the demand for harmful element analysisin Reduning injection.关键词：microwave digestion;ICP-MS;Reduning injection;Pb;As;Cd;Hg;Cu
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发布时间：2024-01-04 - 摘要：Objective: Study the compatibility based on the interaction of multiple components for Sanguisorbae Radix and Sophorae Flos, and to optimize the excellent compatibility proportion. Method: Different compatibility proportion of Sanguisorbae Radix and Sophorae Flos were designed, and then extract them with hot water, the contents of total flavonoids and total tannins in each extract were determined using ultraviolet spectrophotometric method, and the DPPH radical scavenging activity was used to evaluate the antioxidant capacity, and then analyzed the relationship between DPPH radical scavenging activity and the content of total flavonoids and total tannins. Meanwhile, the HPLC fingerprints of different extracts were tested and comparative analyzed the difference between them. Result: The contents of total flavonoids and total tannins of each extracts were different, and the DPPH scavenging activity was also different. When the compatibility proportion of Sanguisorbae Radix-Sophorae Flos was 2: 1 and 1: 1, the DPPH scavenging activity was better, and the comprehensive score of the contents of total flavonoids and total tannins was the highest, which suggested that the DPPH scavenging activity was positively related to the contents of total flavones and total tannins. And the fingerprints of different extracts were different, which indicated that the compatibility proportion had some influences on chemical components. Conclusion: The contents of total flavonoids and total tannins were higher, and the DPPH scavenging activity was better than others at the compatibility proportion was 2: 1 or 1: 1 of Sanguisorbae Radix and Sophorae Flos. The experimental results can be used as a consideration of the compatibility of Sanguisorbae Radix and Sophorae Flos in clinical.关键词：Sophorae Flos;Sanguisorbae Radix;chemical constituents;compatibility medicines
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for simultaneous determination of geniposide, phillyrin, 5-O-methylvisammioside, baicalin, baicalein, wogonin, and ammonium glycyrrhizinate in Fangfeng Tongsheng pill. Method: The chromatographic separation was on phenomenex Luna 5u-C18(4.6 mm×250 mm, 5 μm) with a mobile phase consisting of methanol (A)-0.1% phosphoric acid solution (B) in a gradient mode at a flow rate of 0.8 mL · min-1. The detection wavelengths were 234 nm and 254 nm. Result: Within the linear range of geniposide, phillyrin, 5-O-methylvisammioside, baicalin, baicalein, wogonin, ammonium glycyrrhizinate, the correlation coefficients were lager than 0.999 1.The average recoveries of the seven components were in the range of 97.3%-99.8%. RSD were less than 2.0%. Conclusion: By method validation, this method is proved to be simple, rapid, sensitive and accurate. It can be used to determine the contents of geniposide, phillyrin, 5-O-methylvisammioside, baicalin, baicalein, wogonin, and ammonium glycyrrhizinate in Fangfeng Tongsheng pill.关键词：Fangfeng Tongsheng pill;geniposide;phillyrin;5-O-methylvisammioside;baicalin;baicalein;wogonin;ammonium glycyrrhizinate
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发布时间：2024-01-04 - 摘要：Objective: To study pharmacokinetic characteristics on paeoniflorin in Xuefu Zhuyu Tang in rats. Method: The paeoniflorin content in serum of rats was determined by HPLC.Diamonsil C18 column (4.6 mm×250 mm,5 μm) was adopted and eluted with the mobile phase of acetonitrile-water (16: 84).The flow rate was 1.0 mL · min-1,the temperature of column was 30 ℃,the detection wavelength was 230 nm,and the sample size was 20 μL with riboflavin as the internal standard. Result: The linear relationship was good with the paeoniflorin concentration at 0.01-1.0 mg · L-1.The limit of quantitation was 0.01 mg · L-1.The average intra-day precision was 3.3%,while the average inter-days precision was 3.9%,the average recovery rate was 101.3%.After rats were administered with Xuefu Zhuyu Tang,the peak concentration of paeoniflorin was(0.363±0.080) mg · L-1,with Tmax of (0.276±0.084) h,Ka of (30.905±10.114) h-1,Ke of (1.638±0.181) h-1,t1/2 of (0.501±0.038) h,CL/F of (69.846±4.624) L · h-1 · kg-1,Vz/F of (36.521±12.287) L · kg-1and AUC0-t of (0.356±0.024) mg · L-1 · h. Conclusion: Paeoniflorin in Xuefu Zhuyu Tang is rapidly absorbed,distributed in a large volume and short in half-life,this study can provide a reference for studying effect of compatibility on paeoniflorin's pharmacokinetics and efficacy of Xuefu Zhuyu Tang.关键词：Xuefu Zhuyu Tang;paeoniflorin;pharmacokinetics;riboflavin
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of extracts from Taxus chinensis on cell growth and apoptosis of breast cancer cells T47D. Method: In the experiment, the blank group, different concentrations of T. chinensis extracts groups (200, 100, 50, 25 mg · L-1) and the Adriamycin group (3 μmol · L-1) were established to treat breast cancer cells T47D for 24 h. The T47D cell proliferation was determined by MTT method. The activity of lactate dehydrogenase (LDH) and Caspase-3 were detected by kit. The cell apoptosis was tested by Hoechst33342 fluorescence. The expressions of proteins related to T47D cell apoptosis, such as pro-apoptosis proteins(Bax), anti-apoptotic proteins (Bcl-2) and Caspase-3, were detected by Western blot method. Result: Compared with the blank group, extracts from T. chinensis significantly inhibited the proliferation of T47D cells, improved the LDH and Caspase-3 activities, induced the apoptosis of T47D, up-regulated the level of Bax and down-regulated the level of Bcl-2, with significantly statistical differences (P<0.05, P<0.01) and concentration dependence. Conclusion: Extracts from T. chinensis have a direct inhibitory effect on breast cancer cells T47D proliferation, with concentration dependence. Its mechanism may be related to the induction of cell apoptosis.关键词：extracts from Taxus chinensis;breast cancer;T47D;cell proliferation;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Changweiqing on survival duration, tumor growth and copper-transporting tumor tissues-related protein expression of nude mice in colon carcinoma liver metastasis model, in order to discuss the synergistic mechanism of Changweiqing on the chemotherapy for colorectal cancers. Method: BALB/C nude mice were selected to establish the nude mice colorectal cancer hepatic metastasis model and randomly divided into the model group, the Changweiqing group, the chemotherapy group and the Changweiqing plus chemotherapy (combination) group, with 20 mice in each group. The Changweiqing group was orally administered with corresponding drugs and intraperitoneally injected with normal saline.The chemotherapy group was intraperitoneally injected with oxaliplation(L-OHP) and 5-fluorouracil(5-FU) and orally administered with normal saline.The combination group was orally administered with Changweiqing and intraperitoneally injected with L-OHP and 5-FU;The model group was orally administered and intraperitoneally injected with normal saline. All group were treated for three weeks. The effect of the Changweiqing on survival duration and tumor growth of colorectal cancer hepatic metastasis in nude mice was observed. Human copper transporter 1(hCTR1), ATPase Cu2+ transporting alpha polypeptide(ATP7A), copper transporting P-type adenosine triphosphotase(ATP7B), multidrug resistanceassociated protein 2(MRP2) and glutathione-S-transferase-π(GST-π) protein expressions in tumor tissue were detected by the immunohistochemical method. The apoptosis rate of transplanted tumor tissues was observed by TUNEL method. Result: The Changweiqing group, the chemotherapy group and the Combination group revealed the tumor inhibition rate of 45.33%, 70.51%, 72.07%, particularly the Combination group (P<0.01).Those group showed a longer nude mice survival time compared with the control group (P<0.05).The Changweiqing group and the Combination group showed higher hCTR1 but lower ATP7A, ATP7B, MRP2, GST-π protein expressions in nude mouse tumors than the model group and the chemotherapy group (P<0.05).The Combination group displayed a higher apoptosis index than the chemotherapy group and the model group(P<0.01). Conclusion: The Changweiqing group can up-regulate the expression of hCTR1 in liver metastases tumor tissues, down-regulate ATP7A and ATP7B protein expressions, reduce GST-π and MRP2 expressions, increase platinum content in tumor tissues, induce apoptosis and increase the apoptosis rate, so as to enhance the chemosensitivity.关键词：Changweiqing;colorectal cancer;survival duration;copper transport protein;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To observe effects of Yishen Huazhuo recipe (YSHZR) on the synaptic morphology and the expression of Ca2+ related kinase, calmodulin kinase Ⅱ (CaMKⅡ) and protein kinase C (PKC), in the hippocampus of Alzheimer's disease (AD) rat, and explore the mechanism of YSHZR on the treatment of AD. Method: Fifty male SD rats were randomly divided into the sham group, model group, low, middle and high dose group of YSHZR. The β-amyloid fragment 25-35 (Aβ25-35) was injected into the left lateral ventricle of rats to establish AD rat model. Each dose group was treated by oral administration with 2.8, 5.6, 11.2 g · kg-1 of YSHZR, sham group and model group were given an equal volume of normal saline, and gavaged once daily for 4 weeks. The morphological changes of synapses in the rat hippocampus were observed by an electron microscope, and Western blot was used to measure the expression of CaMK Ⅱ and PKC in the rat hippocampus. Result: Compared with the sham group, the synaptic cleft in the hippocampus of model group rats was in fusion and blurred, presynaptic components were in severe edema, synaptic vesicles were severe reduced, the size was uneven, the vast majority of mitochondrial membrane and crest was in fusion, blurred and even disappeared. The crest mitochondrial was fractured and had cavitation. However, the three doses of YSHZR improved synaptic morphology to some extent, especially the low-dose group. In addition, compared with the model group, the number of synapses, gap of which was clear, was significantly increased (P<0.05) in low dose group of YSHZR;the number of synapses, gap of which showed partly fusion, had no significant change in each dose group of YSHZR;but the number of synapses, gap of whichshowed fully fusion, was significantly reduced (P<0.05). Western blot assay found that, compared with the sham group, the expression of CaMK Ⅱ and PKC in the rat hippocampus of model group was significantly decreased (P<0.05), three dosages of YSHZR could significantly up-regulate the expression of CaMK Ⅱ and PKC (P<0.05). Conclusion: YSHZR may treat AD through improving the synapses morphology and promoting the expression of CaMKⅡand PKC in the hippocampus.
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-myocardial ischemia effect of Danshen oral solution. Method: Totally 70 SD rats were randomly divided into seven groups, with 10 rats in each group. Specifically, they were the control group, the model control group, the Aspirin group (50 mg · kg-1), the compound Danshen dripping pills group (135 mg · kg-1), the Danshen tablets group (300 mg · kg-1), Danshen oral solution high and low dose groups (5, 2.5 g · kg-1). They were orally administered with corresponding drugs for 7 days. One hour later after the last administration, the normal group was injected with physiological saline (5 mL · kg-1) through femoral veins, while the other groups were included into the blood stasis model by being injected with 10% high molecular dextran (5 mL · kg-1). Another 70 rats were randomly divided into seven groups, with 10 rats in each group. Specifically, they were the control group, the model control group, the Isosorbide mononitrate group (4 mg · kg-1), the compound Danshen dripping pills group (135 mg · kg-1), the Danshen tablets group (300 mg · kg-1), Danshen oral solution high and low dose groups (5, 2.5 g · kg-1). They were orally administered with corresponding drugs for 7 days. One hour later after the last administration, the normal control group was sublingually injected with normal saline, while the other groups were included into the myocardial ischemia model rats by being sublingually injected with pituitrin 0.5 U · kg-1 (1 U · mL-1). The blood and plasma viscosities of rats in the blood stasis model were detected by blood viscometer. The changes of T wave in electrocardiogram(ECG) at different time points of rats in the blood stasis model were recorded, and creatine kinase (CK) and malonyldialdehyde (MDA) in plasma, superoxide dismutase (SOD), Ca2+ and MDA in plasma were determined. Result: Compared with the normal group, the blood and plasma viscosities of the model group increased significantly (P<0.01), T wave at different time points in model rats increased significantly (P<0.05, P<0.01), heart rate, MDA and CK in plasma of model rats increased significantly (P<0.01), MDA and Ca2+content in myocardial tissues of model rats increased significantly (P<0.01), SOD in myocardial tissues of model rats decreased significantly (P<0.05). Compared with the model group, Danshen oral solution high and low dose groups significantly reduce the blood and plasma viscosities in blood stasis rats (P<0.05, P<0.01) and T wave and heart rate in myocardial ischemia rats (P<0.01), MDA, CK in plasma (P<0.05, P<0.01) and MDA and Ca2+ content in myocardial tissues of rats (P<0.05, P<0.01) and significantly increase SOD (P<0.05). Conclusion: Danshen oral solution has the protective effect in promoting circulation and removing blood stasis among blood stasis model rats and myocardial ischemia rats.关键词：Danshen oral solution;blood stasis;myocardial ischemia;promoting blood circulation and removing blood stasis
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-tumor effect and pathological morphology change of phenolic alkaloids of menispermum dauricum (PAMD) in pancreatic cancer cell line BxPC-3 transplanted into nude mice. Method: The 40 metastatic tumor model of tumor-bearing mice was established by injecting BxPC-3 cell line hypodermically at right oxter. After 24 h, the mice were randomly divided into 5 groups:the normal group (normal saline), the model group (normal saline), the 5-FU group (20 mg · kg-1), the high-and low-dose PAMD groups (20, 10 mg · kg-1) of 8 mice each. The mice except the normal group received heterotopic tumor transplantation of pancreatic carcinoma cell BxPC-3 subcutaneously. The corresponding medicines were intraperitoneally administered to mice for 20 days. Histomorphology changes of transplanted tumor tissue were observed by using HE staining. The ultrastructure of areas was observed by using electronicmicroscopy. Result: Compared with the normal group the tumor growth of tumor-bearing mice increased, pathological morphology and ultrastructure had obvious changes in the model group (P<0.01). Compared with the model group, the tumor growth inhibited, the inhibition rates were 51.67%, 37.26%, 33.58%, respectively, in the high-, low-dose PAMD and 5-FU groups (P<0.05). Moreover, pathological morphology and ultrastructure had good improvement. Conclusion: PAMD could effectively suppress tumor-bearing nude mice tumor cell growth of pancreatic cancer cell line BxPC-3 and improve the morphology of transplanted tumor tissue.关键词：phenolic alkaloids of Menispermum dauricum;pancreatic cancer;BxPC-3;morphology;nude mouse
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect and related mechanism of tetrahydroxystilbene glucoside (TSG) on SH-SY5Y cells damaged by 6-hydroxydopamine(6-OHDA). Method: The SH-SY5Y cell damage model was established with 100 μmol · L-1 6-OHDA. The SH-SY5Y cells were pretreated by TSG with the concentrations of 6.25,12.5, 25, 50, 100, 200 μmol · L-1. The blank group was also set up. The protective effect of different concentrations of TSG on cell viability was measured by MTT assay. The changes in the concentration of intracellular calcium was observed by the laser scanning confocal microscopy. The concentration of excitatory amino acids glutamate (Glu) and inhibitory amino acid γ-aminobutyric acid (GABA) in supernatant of different-treated cells were tested by HPLC-FLD. Result: Compared with the blank group, the model group showed lower cell viability and higher free calcium (P<0.01) and extracellular Glu and GABA concentrations(P<0.01). Compared with the model group, the 24-hour pre-treatment with TSG can inhibit the decrease in the cell viability caused by 6-OHDA, with the most significant protective effect in 12.5 μmol · L-1 (P<0.05), decrease intracellular free calcium content and extracellular Glu and GABA concentrations(P<0.05,P<0.01). Conclusion: TSG can protect SH-SY5Y cells damaged by 6-OHDA by relieving calcium overload and excitatory toxicity.关键词：2,3,5,4'-tetrahydroxystobene-2-O-β-D-glucoside;6-hydroxydopamine;calcium ion;amino acid
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity of different fractions and chemical constituents from Semiaquilegiae Radix in vitro. Method: Different extraction fractions from Semiaquilegiae Radix were ethyl acetate extraction(TK-Y), n-butanol extraction(TK-Z), water extraction(TK-S) and polysaccharide(TK-D). Under the chemical simulation conditions in vitro, the antioxidant activities of different extraction fractions and polysaccharide were determined by · OH, O2- · scavenging experiments. Furthermore, the chemical compounds were isolated from bio-active fraction. Result: The antioxidant activities of different extraction fractions from strong to weak were as follows:TK-D>TK-S>TK-Z>TK-Y.Scavenging activity of TK-D for O2- · and · OH were positively correlated (Y=54.94X+27.15, R2=0.998 3), IC50=0.42 g · L-1.Five major components were isolated and identified from TK-Z as lithtospermoside(1), thalictricoside(2), griffonilide (3), β-sitosterol(4), daucosterol(5). Conclusion: The polysaccharide, n-butanol extraction and water extraction are active fractions and it identified the active fractions of Semiaquilegiae Radix for the first time. Lithtospermoside, thalictricoside and griffonilide were found as main contribution.关键词：Semiaquilegiae Radix;antioxidant;·OH;O2- ·chemical compounds
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity of cyclophosphamide (CTX) combined with ethyl acetate extracts from persimmon leaves (PE) on hepatoma H22 bearing mice and their effect on tumor tissue apoptosis-related proteins Bcl-2 and Bax expressions. Method: Kunming mice were selected and subcutaneously inoculated with H22 tumor at right fore armpit to establish the H22 bearing mouse model. The H22 bearing mice were randomly divided into the model group, the CTX group (0.1 g · kg-1), the CTX combined with high, middle and low dose PE groups (2, 1, 0.5 g · kg-1) and were orally given drugs for 10 days. After killing the rats, efforts were made to calculate the anti-tumor rate, detect the combined effect by Jin's formula q, superoxide dismutase(SOD) activity and malonaladehyde(MDA) content in tumor tissues, and analyze Bcl-2 and Bax protein expressions in tumor tissues by immunohistochemistry. Result: Compared with the model group, all treatment groups showed significantly higher tumor inhibition rate, SOD and Bax protein expression and lower MDA and Bcl-2 protein expression (P<0.05, P<0.01). The tumor inhibition rate of CTX combined with PE in each dose group was higher than that of the CTX group, with the Jin's formula q between 0.85 and 1.15, indicating their combined effect. Compared with the CTX group, the SOD activity and bax expression of CTX combined with PE in each dose group increased, but the MAD content, Bcl-2 expression and Bcl-2/Bax ratio decreased (P<0.05, P<0.01). Conclusion: CTX combined with PE had better antitumor effect. Its mechanism is probably related to their combined effect in notably strengthening the antioxidant capacity, down-regulating Bcl-2 expression and up-regulating bax expression of H22 bearing mice.关键词：persimmon leaf;ethyl acetate extract;H22 bearing mice;antioxidant capacity;Bcl-2;Bax
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Pien Tze Huang on nonalcoholic fatty liver disease in rats and to investigate its mechanism. Method: Forty-eighty Wistar rats were randomly divided into 6 groups:the normal group, the model group, the simvastatin group (0.003 g · kg-1 · d-1), the low-, medium-and high-dose Pien Tze Huang groups (0.5, 1,2 g · kg-1 · d-1). The nonalcoholic fatty liver disease model was induced in rats by feeding a high fat diet. After 4 weeks, related specimens were collected and the liver function indexes including aspartate amino transferase (AST), alanine aminotransferase (ALT), albumin (Alb), total bilirubin (TBIL), γ-glutamyl transpeptidase (γ-GT), lipid indexes including total cholesterol (TCH), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) were detected by biochemical analyzer. The mRNA expressions of farnesoid X receptor (FXR), small heterodimer partner (SHP) and sterol regulatory element binding proteins-1c (SREBP-1c) were measured by RT-PCR. The liver histopathological changes were observed by HE staining. Result: Compared with the normal group, the levels of AST, ALT, γ-GT and LDL increased, the mRNA expressions of FXR, SHP decreased, the mRNA expression of SREBP-1c increased and the liver histopathological had varying degrees of injuries in the model group (P<0.01). Compared with the model group, the levels of AST, ALT, γ-GT and LDL decreased, the mRNA expressions of FXR, SHP increased, the mRNA expression of SREBP-1c decreased and the liver histopathological injuries had good improvement in the low-and medium-dose Pien Tze Huang groups (P<0.05, P<0.01). Conclusion: Pien Tze Huang, especially the low and medium dose, is beneficial to treating fatty liver by improving the liver function and reducing blood lipid, which may be related to regulating the pathway of FXR-SHP-SREBP-1c.关键词：Pien Tze Huang;nonalcoholic fatty liver disease;farnesoid X receptor;small heterodimer partner;sterol regulator element binding protein-1c
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Qianyang Yuyin granules(QYG) on renal microcirculation in normal dogs and to investigate the mechanism. Method: The renal microcirculation was determined by PowerLab system before giving QYG (3.08, 6.15,12.30 g · kg-1)and after 15,30,60,90, 120, 180 min respectively. Result: Compared with the normal group,QYG(12.30 g · kg-1)could increase the renal microcirculation(P<0.05,60-180 min), the contents of nitric oxide synthase (eNOS)(P<0.05) and nitric oxide(NO) in the serum(P<0.05,90-180 min) and reduce the contents of angiotensinⅡ(AngⅡ)in the serum(P<0.05,90-180 min)and kidney (P<0.05,180 min);QYG(6.15 g · kg-1) could increase the renal microcirculation(P<0.05,90-120 min), the contents of eNOS and NO in the serum(P<0.05,90-120 min)and reduce the contents of Ang Ⅱ in the serum(P<0.05,90-120 min). The effects of QYG(3.08 g · kg-1) were not obvious. Conclusion: QYG could improve renal microcirculation, its mechanism may be associated with the decreasing circulation and kidney tissue AngⅡ content, increasing the circulation of eNOS and NO contents.关键词：Qianyang Yuyin granules;normal dogs;renal microcirculation;angiotensin Ⅱ
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Jiawei Yinchenhao decoction on the acute airway inflammation and its possible mechanism in the of dampness-heat asthma rat. Method: Fifty healthy male SD rats were randomly divided into five groups:control group, dampness-heat asthma model group and Jiawei Yinchenhao decoction low dose group, Jiawei Yinchenhao decoction high dose group, dexamethasone group, 10 rats in each group.The dampness-heat asthma model was induced by multi-factors. The rats in dampness-heat asthma groups were fed with medicine from 16th day of the experiment. The rats were killed within 24 h after the last asthma provocation test to collect abdominal aortic blood and lung tissue specimens for testing indexes. The bronchoalveolar lavage fluid(BALF) was collected,the total cell and the cell differentials were counted,the level of interleukin-2(IL-2),IL-4,IL-5,IL-6, and interferon-γ(IFN-γ) in BALF were measured by ELISA.The expression of signal transducers and activators of transcription 3 (STAT3) in airway was detected by immunohistochemistry technique.With glyceraldehyde-3-phosphate dehydrogenase(GAPDH)being a control, airway tissue extracts were used for detecting STAT3/GAPDH expression level by reverse transcription-polymerase chain reaction(RT-PCR). SPSS 17.0 software was used to analyze the data. Result: Compared with the control group, the level of IL-2 and IFN-γ in BALF of the other groups was declined while IL-4,IL-5 and IL-6 level was higher than that in the control group. Compared with the dampness-heat asthma group,the IL-4,IL-5 and IL-6 level in treatment groups was significantly lower than that in the model group(P<0.05),while IL-2,IFN-γ was higher. The level of IL-6,STAT3 and STAT3/GAPDH had positive correlations with IL-4,IL-5 and IL-6 in BALF(P<0.05) and negative correlations with IL-2,IFN-γ.All the treatment group could reduce airway inflammation. Conclusion: Clearing heat and removing dampness and phlegm mothed could reduce the acute airway inflammation, its efficacy is dose-related,the Chinese medicine low dose group has better effect. Its mechanism may be related to inhibition of IL-6/STAT3 signaling pathway to regulate the expression of Thl/Th2 cytokine imbalance.关键词：dampness-heat asthma;interleukin-6/signal transducers and activators of transcription 3;airway inflammation
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发布时间：2024-01-04 -
Effects of Yulin Decoction on Secretion Function of Granulosa Cells of Ovarian Reserve Declined Rats
摘要：Objective: To investigate the effect of Yulin decoction on the secretion function of granulosa cells in cytoxan-induced (CTX) ovarian reserve declined rats. Method: The rats were randomly divided into 6 groups:the normal group (distilled water, 20 mL · kg-1), the model group (distilled water, 20 mL · kg-1), the progynova group (0.21 mg · kg-1), the high-, medium-and low-dose Yulin decoction groups (15.2, 30.4, 60.8 g · kg-1) of 10 rats each. The ovarian reserve declined rats model were induced by injecting CTX intraperitoneally. The corresponding medicines were intragastrically administered rats for 21 days. Histomorphology changes of ovaries, the numbers of follicle and corpus luteum were observed, serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-mullerian hormone (AMH) were determined by radioimmunoassay. Result: Compared with the normal group, the levels of FSH and LH increased, AMH level decreased, the numbers of follicle and corpus luteum decreased in the model group (P<0.05). Compared with the model group, the levels of FSH and LH decreased in Yulin decoction groups, AMH level increased in the medium-dose Yulin decoction and progynova groups, the numbers of follicle and corpus luteum increased in the medium-and high-dose Yulin decoction groups (P<0.05). Conclusion: Yulin decoction could improve the ovarian reserve function by regulating the secretion function of granulosa cells of ovarian reserve declined rats.关键词：Yulin decoction;decline in ovarian reserve;granulosa cells;cytoxan- 3
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发布时间：2024-01-04 - 摘要：Objective: To compare the effects of Sishen pills and Gegen Qinlian tablets in treating acute or chronic colitis induced by dextran sulfate sodium (DSS) in mice. Method: In the acute or chronic colitis experiment, C57BL/6J mice were randomly divided into four groups: the normal group, the DSS acute or chronic model group, the Sishen pills group (2.25 g · kg-1) or Gegen Qinlian tablets group (6.5 g · kg-1). The acute colitis model was established by free drinking of 4%DSS for five days, while the chronic colitis model was established by circulating drinking of 3%DSS (1-5, 8-12, 15-19 d and 22-26 d). The acute model was orally given drugs on the second day after drinking DSS for 8 d, while the chronic model was given drugs after the second circulating drinking for 16 d. The disease activity index (DAI) and myeloperoxidase (MPO) in colonic tissues were respectively determined, and the changes in colonic tissue were detected by HE staining. Result: Compared with the normal group, both acute colitis and chronic colitis groups showed significant higher DAI and MPO and notable inflammatory damages in colonic tissues (P<0.05, P<0.01). In the acute DSS model, Gegen Qinlian tablets had an effect on reducing DAI since the 5th day, while Sishen pills significantly reduced DAI since the 7th day (P<0.05),both can significantly relieve inflammatory damages in colonic tissues (P<0.01, P<0.05) and reduce MPO activity in colonic tissues (P<0.05, P<0.01). In the chronic DSS model, Sishen pills significantly reduced DAI since the 18th and 24th day (P<0.05) and relieve inflammatory damages in colonic tissues (P<0.05), while Gegen Qinlian tablets had no significant change in all indexes of rats with chronic colitis. Conclusion: Sishen pills shows a remarkable effect in ameliorating acute colitis and chronic colitis induced by DSS, while Gegen Qinlian tablets can only ameliorate acute colitis.关键词：Sishen pills;Gegen Qinlian tablets;colitis;dextran sulfate sodium
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发布时间：2024-01-04 - 摘要：Objective: To investigate the apoptotic effect of rhein on human proximal tubular epithelial cell line (HK-2) and the underlying mechanisms. Method: HK-2 cells were seeded in 96-well plates at an initial density of 4× 104 cells/mL for 24 h, and then the four different concentration of rhein (0, 25, 50, 100 μmol · L-1) were applied to test cells for 12, 24, 48 h respectively. MTT assay was used to detect the viability of HK-2 cells. Rhein-induced apoptosis in HK-2 cells was evaluated by Hoechst 33258 staining and Flow cytometry. The expression of proto-oncogene c-jun(c-jun),activating transcription factor 2(ATF-2) and cysteine proteinases with specificity for aspartic acid residues 3(Caspase-3) mRNA was detected by real-time quantitative polymerase chain reaction (RT-qPCR).The changes of phosphorylated c-jun N-terminal kinase (p-JNK), c-jun N-terminal kinase(JNK), phosphorylated p38 mitogen-activated protein kinase(p-p38 MAPK), p38 mitogen-activated protein kinase (p38 MAPK) and Cleaved Caspase-3 protein were detected by Western blotting. The specific inhibitors of JNK and p38 MAPK were applied for identifying the roles of the corresponding signal pathways in rhein-induced apoptosis of HK-2 cells. Result: Rhein inhibited the viability of HK-2 cells in a dose-and time-dependent manners. The apoptosis rate of HK-2 cells in rhein groups was apparently increased. The RT-qPCR found that the expression of c-jun, ATF-2, Caspase-3 mRNA was significantly increased in rhein-treated groups. The results of Western blotting showed that there was no significant change in p38 and JNK protein, but an increasing trend in p-p38, p-JNK and Cleaved Caspase-3 was observed. After treatment with SP600125 (an inhibitor of JNK) and SB203580 (an inhibitor of p38) respectively, the apoptosis rates of rhein on HK-2 cells were significantly weak. Conclusion: Rhein induces apoptosis involving MAPK signal transduction pathway in HK-2 cells.关键词：rhein;HK-2 cells;apoptosis;mitogen-activated protein kinase signal transduction pathway;c-Jun N-terminal kinase;p38 mitogen-activated protein kinase
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effect of Qishen Yiqi pills on heart function and hypersensitive C-reactive protein in patients with chronic heart failure. Method: Totally 70 patients with chronic cardiac failure were randomly divided into the control group (35 cases) and the treatment group (35 cases) by the digital table method. Both groups received the standard chronic heart failure therapies, ie oral administration with Cefuroxime tablets (20 mg · d-1, qd), Benaaepril Hydrochleride tablets (10 mg · d-1, qd) and Digaoxin tablets (for patients without contraindication, 0.125 mg · d-1, qd). On the basis of the conventional therapies, the treatment group was additionally given Qishen Yiqi pills (0.5 g, tid). Both groups were treated for eight weeks. Their clinical efficacy, traditional Chinese medicine(TCM) syndrome score,left ventricular and systolic diameter(LVESD), left ventricular and diastolic diameter(LVEDD),left ventricular ejection fraction(LVEF), b-type natriuretic peptide(BNP), 6-min walking test before and after the treatment were observed. Meanwhile, the content of hypersensitive C-reactive protein before and after the treatment was detected. Result: After the 8-week treatment, the treatment groups showed a better clinical efficacy and higher TCM syndrome score and total effective rate than the control group. Both groups showed statistical difference in heart color ultrasound, BNP, hypersensitive C-reactive protein and 6-min walking test (P<0.05). Conclusion: Qishen Yiqi pills can reduce the serum hypersensitive c-reactive protein level by inhibiting the expression of inflammatory factors, further improve heart function of patients with chronic cardiac failure and enhance the clinical efficacy.关键词：chronic heart failure;Qishen Yiqi pills;b-type natriuretic peptide;hypersensitive c-reactive protein
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical therapeutic effect of Yangxue Sanhan granule in treating the diabetic peripheral neuropathy (Qi and blood deficiency, cold-dampness syndrome). Method: Totally sixty patients with diabetic peripheral neuropathy were randomly divided into the treatment group and the control group and treated for the basic hypoglycemia. The treatment group was administered with Yangxue Sanhann granules, while the control group was given mecobalamine tablets. The study lasted for 12 weeks. The traditional Chinese medicine(TCM) symptom score and nerve conduction velocity of both groups were detected during and after the study. Result: The total effective rate of the treatment group was 72.41%, and that of the control group was 14.29%, with statistical differences (P<0.05). Both groups showed lower TCM symptom scores compared with their own scores before and after the treatment (P<0.01) and statistical differences in TCM symptom scores after the treatment (P<0.01), without intra-group and inter-grioup significant difference in the nerve conduction velocity. Conclusion: Yangxue Sanhan granules can treat the diabetic peripheral neuropathy (Qi and blood deficiency, cold-dampness syndrome), effectively improve the clinical symptoms, particularly such symptoms as pain and fatigue, with a better comprehensive clinical efficacy than mecobalamine tablets. However, both groups showed no notable improvement in the nerve conduction velocity.关键词：Yangxue Sanhan granules;Qi and blood deficiency;cold-dampness syndrome;diabetic peripheral neuropathy
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发布时间：2024-01-04 - 摘要：Objective: To study the efficacy of Qingfei Xiaotan Jiejing decoction with salbutamol aerosol in treating asthma. Method: Altogether one hundred and twenty-eight patients with bronchial asthma (cold asthma) treated in our hospital from February 2010 to May 2014 were selected as the study objects and randomly divided into the observation group and the control group, with 64 cases in each group. Both groups of patients were given regular salbutamol aerosol with the dose of 100 μg/time, twice in the morning and evening. On that basis, the observation group is orally given traditional Chinese medicine Qingfei Xiaotan Jiejing decoction, one dose a day, taken warm in the morning and evening. Patients of the two groups were tested for their lung ventilation function, peak expiratory flow (PEF), asthma control test score (ACT) and traditional Chinese medicine (TCM) symptoms integral before the treatment and at the 12th week after the treatment. A comparative analysis was made for its clinical efficacy according to the above results. Result: Various observation indexes in both groups were obviously improved after treatment compared with that before the treatment. Specifically, compared with the control group, the observation group showed significantly increase in forced expiratory volume in one second (FEV1), PEF, ACT, clinical control rate and notable decrease in TCM syndrome integrals and inefficiency, with statistical differences. Conclusion: Qingfei Xiaotan Jiejing decoction combined salbutamol aerosol can effectively improve the pulmonary ventilation function, relieve the clinical symptoms and enhance the clinical control rate of patients with bronchial asthma (cold asthma), with a better efficacy than the single administration of salbutamol aerosol.关键词：bronchial asthma;cold asthma;Qingfei Xiaotan Jiejing decoction;combination of traditional Chinese medicine and Western medicine
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Bogu Huoxue decoction combined with glucosamine hydrochloride tablets in treating knee osteoarthritis (KOA) and its influence on interleukin-1β(IL-1β), tumor necrosis factor-α (TNF-α), matrix metalloproteinases-3 (MMP-3), matrix metalloproteinase inhibitors-1 (TIMP-1). Method: Totally 96 patients with KOA were randomly divided into the control group and the observation group, with 48 patients in each group. The control group was orally administered with glucosamine hydrochloride tablets (480 mg/time, tid). Based on the therapy of the control group, the observation group was additionally given Bogu Huoxue decoction (1 dose/d). Both groups were treated for six weeks. The visual analogue scale (VAS) score and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score were recorded before and after the treatment. The levels of IL-1β,TNF-α, MMP-3,TIMP-1 of the patients were detected before and after the treatment. Result: The obvious curative rate was 72.92% in the observation group, which was higher than 39.58% of the control group (P<0.05). The VAS scores in both groups were lower than before the treatment (P<0.01). The VAS score in the observation group was obviously lower than that of the control group at the 3rd and 6th week after the treatment (P<0.01). The score for daily activities, pain score, joint stiffness score and total integral of the observation group were lower than that of the control group (P<0.01). The TNF-α, IL-1β,MMP-3 in the synovial fluid in the observation group were lower than that in the control group (P<0.01), but with the level of TIMP-1 higher than the control group (P<0.01). Conclusion: Bogu Huoxue decoction combined with glucosamine hydrochloride tablets in the treatment of KOA can significantly relieve postoperative arthralgia and ankylosis and improve daily movement, with superior clinical efficiency to glucosamine hydrochloride tablets. Its mechanism may be related to the inhibition of the levels of IL-1β, TNF-α, MMP-3 in the synovial fluid and the increase in TIMP-1.关键词：Bogu Huoxue decoction;knee osteoarthritis;interleukin-1β;tumor necrosis factor-α;matrix metalloproteinases-3;matrix metalloproteinase inhibitors-1
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Shenqi Jiangtang capsule in regulating sugar and lipid metabolism of patients with type 2 diabetes combined with dyslipidemia and on the levels of serum tumor necrosis factor-α (TNF-α), adiponectin (APN), leptin (LP), hypersensitive c-reactive protein (hs-CRP). Method: Totally 106 patients were randomly divided into the observation group (54 cases) and the control group (52 cases). Both groups were given Metformin Hydrochloride tablets and Acarbose Tablets to control blood sugar. The control group took Atorvastatin tablets (20 mg · d-1, taking before sleeping).The observation group took Shenqi Jiangtang capsule (3 pieces/time, tid). Both groups were treated for 12 weeks. Before and after the treatment, the levels of fasting blood glucose (FBG), 2 hours postprandial blood glucose (PBG) and glycosylated hemoglobin (HbAlc) were detected. The insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein A (ApoA), apolipoprotein B (ApoB), TNF-α, APN, LP, hs-CRP were calculated. A safety evaluation was conducted. Result: According to the Ridit analysis, there was no significantly statistical difference in the blood lipid efficacy between the observation group and the treatment group. After the treatment, the levels of HbA1c, HOMA-IR in the observation group were lower than that in the control group, with higher ISI than that in the control group (P<0.05). The levels of TC, TG, LDL-C and ApoB in both groups declined but HDL-C and ApoA levels increased compared with that before the treatment (P<0.01). The LDL-C level in the observation group was higher than that in the control group (P<0.05), with no statistical difference in other indexes in the inter-group comparison. And the observation group showed lower TNF-α, LP, hs-CRP levels but higher APN than that in the control group (P<0.05). Conclusion: Shenqi Jiangtang capsule has a certain effect in regulating sugar and lipid metabolism and inflammatory factors of patients with diabetes combined with dyslipidemia and improving HOMA-IR, with few side effects.关键词：type 2 diabetes;hyperlipidemia;Shenqi Jiangtang capsule;sugar and lipid metabolism;inflammatory reaction
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Tongmai Yangxin pills and compound Danshen tablets in treating chronic stable angina(Qi-deficency and blood-stasis syndromes) and improving the quality of life. Method: Totally 108 patients were randomly divided into the control group (54 cases) and the observation group (54 cases). The control group took aspirin tablets (100 mg/time, qd), Metoprolol tablets (100 mg/time, bid) and Simvastatin tablets (20 mg/time, qd). Based on the therapies of the control group, the observation group were additionally given Tongmai Yangxin pills (40 pills/time, bid) and compound Danshen tablets (4 pills/time, 2 qd). When necessary, both groups can sublingually take Nitroglycerin tablets (0.5 or 1 tablet/time at the interval of 5 min). Both groups were treated for 12 weeks. During the study, efforts were made to record Angina frequency, score Qi-deficency and blood-stasis syndromes before and after the treatment, detect electrocardiogram before and after the treatment, record nitroglycerin stop and reduction and adopt seattle angina questionnaire (SAQ) to evaluate the quality of life. Result: According to Ridit analysis, the Electrocardiogram efficacy in the observation group was higher than that in the control group (P<0.05). angina efficacy in the observation group was better than that in the control group (P<0.05). The Qi-deficency and blood-stasis syndromes score in the observation group was less than that in the control group at the eighth and twelfth weeks after the treatment (P<0.01), with a higher decline decrease degree than the control group (P<0.01). After the treatment, the nitroglycerin stop and reduction rate in the observation group was 90.74% and higher than 74.07% in the control group (P<0.05). After the treatment, the limited physical activity level, angina stabilization state, angina frequency, treatment satisfaction in the observation group were higher than that in the control group (P<0.05, P<0.01). Conclusion: On the basis of conventional western medicine therapies, Tongmai Yangxin pills and compound Danshen tablets can reduce the angina frequency of patients with chronic stable angina (Qi-deficency and blood-stasis syndromes) and the use of nitroglycerin, relieve Qi-deficency and blood-stasis syndromes and electrocardiogram and enhance the patient's quality of life.关键词：chronic stable angina;Tongmai Yangxin pills;compound Danshen tablets;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Duhuo Jisheng decoction in staging treatment to promote the fracture healing and relieve the osteoporosis of senile osteoporotic simple thoracolumbar compression fracture cases. Method: Totally 94 patients were randomly divided into the control group (46 cases) and the experiment group (48 cases) by treatment methods. The control group was treated by being injected with salcatonin intramuscularly every two days (50 U/time) and administered alendronate sodium enteric-coated tablets (10 mg/time, qd), calcium carbonate and vitamin D3 tablets (1 tablet/day) and lying on hard bed, wearing lumbar replacer, taking lumbar exercises and other conservative western medicine therapies. Based on the therapies of the control group, the experiment group was also treated with Duhuo Jisheng decoction in staging treatment according to the fracture healing stage. Both groups were treated for four months. The vertebral compression and recovery were detected by X-ray film, and the anterior vertebral height, vertebral body angle and kyphotic Cobb angle were recorded. The pain of patients was evaluated by the pain visual analogue scale (VAS). The waist function was evaluated by Japanese orthopaedic association (JOA) score. The dual energy X ray absorptiometry was adopted to measure the bone density of the femoral neck. Result: The clinical efficacy in the experimental group was higher than that in the control group at the fourth month after the treatment according to Ridit analysis (P<0.05). The anterior vertebral height in the experimental group was higher than that in the control group (P<0.01). The vertebral body angle and kyphotic Cobb angle in the experimental group were less than that in the control group (P<0.01). The pain VAS score in the experimental group was inferior to the data in control group at the third and fourth months after the treatment (P<0.01). The pain VAS score in the experimental group declined more than that in the control group (P<0.01). The JOA score and femoral neck BMD in the experimental group were higher than that in the control group (P<0.01). Conclusion: Duhuojisheng decoction in staging treatment on senile osteoporotic simple thoracolumbar compression fracture can accelerate the fracture healing, reduce pain, waist function and enhance bone mineral density, with a superior clinical efficacy to conservative western medicine therapies.关键词：osteoporosis;thoracic and lumbar vertebral compression fracture;Duhuo Jisheng decoction;staging treatment
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of Gualou Xiebai Banxia decoction combined with Tanreqing injection in treating acute attack of chronic pulmonary heart disease (CPHD) and their influence on hypersensitive C-reactive protein (hs-CRP) and N-terminal brain natriuretic peptide precursor (NT-proBNP). Method: Totally 108 patients were randomly divided into the control group (53 cases) and the observation group (55 cases) according to the digital table method. The control group was treated with comprehensive western medicine therapies, including intravenous injection with cefpirome sulfate (2 g/time, qd), oral administration with ambroxol hydrochloride granules (30 mg/time, tid) and of Salmeterol xinafoate aerosol two puffs, (2×25) μg, 2 bid. Based on the therapies of the control group, the observation group was additionally treated with oral administration with Gualou Xiebai Banxia decoction (qd), intravenous infusion with Tanreqing injection (30 mL/time, qd). Both groups were treated for 14 days. Before and after the treatment, New York Heart Association (NYHA) graded and scored main symptoms and signs, tested the forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), peak expiratory flow (PEF), arterial partial pressure of oxygen (PaO2), oxygen saturation (SaO2), hs-CRP and NT-proBNP levels. Result: After the treatment, the total effective rate of cardiac function in the observation group was 90.91% and higher than 77.36% in the control group, but with no statistical significance in their difference. The total effective rate of comprehensive clinical efficacy in the observation group was 89.09% and higher than 69.81% in the control group (P<0.05). The levels of FVC, FEV1, PEF, PaO2 and SaO2 in both group were slightly higher than that before the treatment (P<0.01), with higher PEF, PaO2 and SaO2 in the observation group than that in the control group (P<0.05) and no statistical significance in FVC and FEV1 between the two groups.The cough, expectoration, asthma, oppression in chest and lung rale scores in both groups where notably lower than that before the treatment (P<0.01).Except for asthma, other symptoms and sign scores in the observation group were lower than that in the control group (P<0.01).And the levels of hs-CRP and NT-proBNP in the observation group were lower than that in the control group (P<0.01). Conclusion: Based on the conventional western medicine therapies, Gualou Xiebai Banxia decoction combined with Tanreqing injection can ameliorate symptoms of phlegm, cough, dyspnea and oppression in chest and cardiac and pulmonary functions, relieve the inflammatory response and improve the comprehensive efficacy.关键词：pulmonary heart disease;acute attack;Gualou Xiebai Banxia decoction;Tanreqing injection;hypersensitive c-reactive protein;N-terminal brain natriuretic peptide precursor
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发布时间：2024-01-04 - 摘要：Objective: To study the clinical efficacy of Wenyang Jianpi decoction in treating spleen-kidney yang deficiency type laxative constipation and stem cell factor(SCF)/c-kit signal pathway. Method: The eighty patients with spleen-kidney Yang deficiency type laxative constipation treated in our hospital during January 2013 to September 2014 were randomly divided into the control group (mosapride) and the observation group (Wenyang Jianpi decoction), with 40 patients in each group. After a 4-week treatment, the changes in clinical efficacy, traditional Chinese medicine(TCM) symptom scores before, during and after the treatment were observed;and the protein expressions of colon c-kit and SCF were detected by western blot. Result: Compared with the control group, the total effective rate significantly increased in the observation group, with statistical difference (90.0%VS 70.0%, P<0.05);compared with the tyrosine kinase receptor control group, the TCM symptom scores after the treatment obviously decreased in the observation group, with statistical difference (4.8±2.3 VS 8.2±3.4, P<0.05);compared with the pre-treatment groups, the protein expressions of colon tyrosine kinase receptor (c-kit) and stem cell factor (SCF) in the post-treatment groups significantly increased, with statistical difference (P<0.05);compared with the control group, the protein expression of colon c-kit significantly increased in the post-treatment observation group, with statistical difference (0.39±0.17 VS 0.28±0.15, P<0.05) and the protein expression of colon SCF showed no significant changes and statistical difference (0.72±0.23 VS 0.71±0.20). Conclusion: The Wenyang Jianpi decoction can significantly increase the protein expression of colon c-kit and SCF and relieve the clinical symptoms of patients with spleen-kidney Yang deficiency type laxative constipation through SCF/c-kit signal pathway.关键词：Wenyang Jianpi decoction;spleen-kidney Yang deficiency type;laxative constipation;SCF/c-kit signal pathway
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effectiveness and safety of Wumei decoction in the treatment of ulcerative colitis. Method: Randomized controlled trials (RCTs) about Wumei decoction combined with western medicines and western medicine in treating ulcerative colitis were included. Their qualities were assessed by the Cochrane scale. The therapies, sample size, clinical efficacy, endoscopic improvement and adverse events of the included literatures were collected. A Meta-analysis was made on the efficacy and adverse events. Result: A total of 11 RCTs were included. The results of Meta-analysis showed that compared with the western medicine group, the combined RR of clinical efficacy and endoscopic improvement in the Wumei combined group was 1.57, 95%CI (1.42, 1.75) and 2.26, 95%CI (1.38,3.71), with statistical significance (P<0.05). Both of their adverse events showed no significant difference. The sensitivity analysis and funnel plot analysis showed a higher homogeneity in the literatures and no evidence of publication bias. Conclusion: Compared with the western medicine, Wumei combined with western medicines had a better clinical efficacy and endoscopic improvement and good safety and so is suitable for clinical use. However, due to the low quality of the included literatures and insufficient experimental design, the study needs the support from a large sample-size randomized double-blind controlled trial.关键词：Wumei decoction;ulcerative colitis;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To systematically evaluate the clinical efficacy and safety of Yangxue Qingnao granules in treating chronic cerebral circulation insufficiency (CCCI). Method: The systematical evaluation method was adopted to search Chinese and foreign randomized controlled trails (RCTs) of Yangxue Qingnao granules in treating CCCI, screen out eligible studies and evaluate the quality of the included studies. Heterogeneity test, Meta-analysis, funnel plot analysis and sensitivity analysis were adopted in the statistics of relevant data. Result: The 12 RCTs were selected. The Meta-analysis indicated that the ratio between the combined RR of total effective rate and the 99% confidential interval (CI) was 1.23 (1.14, 1.33). The combined MD of transcranial doppler(TCD) detected carotid artery, vertebral artery, basilar artery blood flows (99%CI) respectively were (2.29,13.94), 2.54 (0.21,4.87), 5.60 (2.35,8.66);PetoOR of the incidence rate of extrapyramidal symptoms and gastrointestinal reaction (99%CI) were 0.11 (0.01,1.77), 8.07 (0.77,84.94), respectively. Conclusion: Meta-analysis shows that Yangxue Qingnao granules has the efficacy in increasing cerebral blood flow of patients with chronic brain and improving their cognitive function, but with uncertain risks in causing adverse reactions. Affected by such factors as lower quality of included studies, the systematic evaluation's conclusion is lowly reliable.关键词：Yangxue Qingnao granules;chronic cerebral circulation insufficiency;systematic evaluation;randomized controlled trial
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effect of Yinchen Wulin powder in treating hyperlipidemia. Method: Randomized control trials for studying the effect of Yinchen Wulin powder in treating hyperlipidemia from January 2000 to January 2013 were retrieved from the Cochrane library, Pubmed, CNKI, Wanfang and VIP database. The inclusion and exclusion standards were followed in screening literatures, extracting materials and evaluating their quality. The study results of the included literatures were analyzed by RevMan 5.2. Result: Totally 10 RCTs were included and involved 711 patients. According to the the results of the Meta-analysis, compared with the control group, the total effective rate of the treatment group increased. In decreasing the cholesterol (TC), Yinchen Wulin powder was similar to statins and betts and superior to other Chinese patent lipid lowering medicines or other intervention factors. In decreasing the triglyceride (TG), Yinchen Wulin powder was superior to statins and other factors and was similar to betts. In increasing the high density lipoprotein (HDL), Yinchen Wulin powder was superior to betts and other factors, and similar to Statins. In decreasing the low density lipoprotein (LDL), Yinchen Wulin powder was superior to betts and other factors, and only next to Statins. Conclusion: Yinchen Wulin powder has a better efficiency in treating hyperlipidemia.关键词：Yinchen Wulin powder;hyperlipidemia;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To analyze pharmacodynamic material basis and mechanism for commonly used anti-diabetic traditional Chinese medicine by molecular docking technology,in order to provide a theoretical basis for modern studies on anti-diabetic traditional Chinese medicines. Method: Chemical components of anti-diabetic traditional Chinese medicines were collected based on literatures to establish the structure database.With seven diabetes therapeutic targets such as dipeptidyl peptidase-IV as the study objects,virtual screening was conducted by the Surflex-Dock module of Sybyl software.With scoring function Total-Score as the standard,interactions between components of traditional Chinese medicine and targets were evaluated.With the Total-Score value at 7,molecules with high score were screened out to further compared with anti-diabetic western medicines in the market in terms of the drug-likeness. Result: There were 52,28,32,150,47,41 and 33 chemical components well combined with dipeptidyl peptidase-IV,glycogen synthase kinase-3,peroxisome proliferator-activated receptor gamma,alpha-glucosidaseare,glucokinase,sodium glucose cotransporter 2 and angiotensin converting enzyme.These small molecule compounds obtained by virtual screening were similar to the western medicine in the market in terms of the drug-likeness. Conclusion: Molecular docking technology can explain pharmacodynamic material basis and mechanism of anti-diabetic traditional Chinese medicines to some extent and provide clues for modern studies on anti-diabetic traditional Chinese medicines.关键词：diabetes;therapeutic target;anti-diabetic traditional Chinese medicine;chemical component;molecular docking;drug likeness
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发布时间：2024-01-04 - 摘要：Objective: To systematically evaluate the efficacy and safety of Yangxue Qingnao granule (YQG) in treating vascular dementia (VD). Method: A computerized literature retrieval was carried out in main Chinese and English databases to collect randomized controlled trials (RCTs) for YQG in treating VD. The quality of all included trials was evaluated according to the Cochrane Handbook 5.1.RevMan 5.3 software was applied in a Meta-analysis on included studies. Funnel plot and Egger's test were used to analyze the publication bias. The quality of evidences was evaluated by GRADE profiler 3.6.1 software. Result: Altogether 10 studies were included. According to the Meta-analysis, compared with the conventional treatment with western medicines, the treatment with YQG showed statistical difference in mini-mental state examination (MMSE) scores [WMD=2.12, 95%CI (1.61, 2.63)], Hasegawa dementia scale(HDS) scores [WMD=3.98, 95%CI (3.16, 4.79)] and activity of daily living (ADL) scores [SMD=-0.62, 95%CI (-0.80,-0.44)]. On the basis of the subgroup analysis on MMSE score, the treatment measures based on the YQG group was superior to that of the donepezil group, the nimodipine group and the piracetam group, with higher safety evaluation and no publication bias. The GRADE profile showed a low quality of evidences. Conclusion: The treatment measures based on YQG was superior to that of the conventional western medicines. However, clinicians shall sue the evidence cautiously due to the low methodology quality, a small number of studies included and lower evidence grade.关键词：Yangxue Qingnao granule;vascular dementia;systematic evaluation;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To analyze the clinical application regularity of Glycyrrhizae Radix et Rhizoma group in 18 incompatible pairs. Method: The application of Glycyrrhizae Radix et Rhizoma group in 18 incompatible pairs in provincial grade 3 class A hospitals in recent three years (November 2009—November 2012) were retrieved, analyzed, summarized and concluded. Result: The order of herbs with the same clinical application frequency with Glycyrrhizae Radix et Rhizoma were as follows:Sargassum> vinegar-Kansui Radix> vinegar-Spurge> vinegar-Genkwa Flos, with Sargassum accounted for over 80%. As for the ratio, except for Sargassum that was consumed more than Glycyrrhizae Radix et Rhizoma (usually two to three times of the consumption of baked Glycyrrhizae Radix et Rhizoma), all of vinegar-Kansui Radix, vinegar-Spurge and vinegar-Genkwa Flos were consumed less than Glycyrrhizae Radix et Rhizoma(mostly 1/10 of the consumption of baked Glycyrrhizae Radix et Rhizoma). Regarding comparable herbs with Glycyrrhizae Radix et Rhizoma group in 18 incompatible pairs, the top six comparable herbs with Glycyrrhizae Radix et Rhizoma group were Poria, Cinnamomi Ramulus,Paeoniae Radix Alba, Moutan Cortex,fired Persicae Semen,Ginseng Radix et Rhizoma Rubra tablets. Conclusion: In the Glycyrrhizae Radix et Rhizoma group in 18 incompatible pairs, the most frequent compatible herbs with Glycyrrhizae Radix et Rhizoma are Sargassum, which is followed by Kansui Radix and Spurge. It suggests that the application of 18 incompatible pairs in the same prescriptions is also an objective exist, instead of an absolute incompatibility. This study will provide certain significance in guiding the clinical application of Glycyrrhizae Radix et Rhizoma group in 18 incompatible pairs.关键词：18 incompatible pairs;compatibility;Glycyrrhizae Radix et Rhizoma;vinegar-Kansui Radix;vinegar-Spurge;vinegar-Genkwa Flos
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发布时间：2024-01-04 - 摘要：To investigate the intervention effect and its mechanism of treatment of cerebral ischemic stroke by Notoginseng Radix et Rhizoma saponins.To analyze and summarize the internal and oversea research in the related area about pharmacological effects and experimental references on treatment of cerebral ischemic stroke by Notoginseng Radix et Rhizoma saponins in recent years.The results suggest that Panax Notoginseng Saponins has obvious protective effects for the cerebral ischemic stroke by several different mechanisms.Notoginseng Radix et Rhizoma saponins and its effective components has obvious inhibiting inflammatory factor expression,toxicity of free-radical damage,anti-platelet aggregation and inhibition of apoptosis control gene,promoting peripheral nerve cell regeneration of ischemic stroke.There for it may become effective drug for the treatment of cerebral ischemic stroke and for its development and utilization are further provides a new thinking.关键词：Notoginseng Radix et Rhizoma saponins;effective component;ischemic stroke;mechanism
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发布时间：2024-01-04 - 摘要：Mulberry Morus alba includes multiple polyhydroxylated alkaloidsi in leaves, branches root barks, which have many pharmacologic effects in reducing blood sugar and blood lipid, resisting virus, enhancing immunity and anti-aging. However, because spolyhydroxylated alkaloids share a similar structure with common carbohydrates in plants, with a strong polarity and alkalinity, they are hard to be separated and purified, which needs complicated separation procedures and multiple column chromatography. Furthermore, there is no literature about stationary phase materials for separating polyhydroxylated alkaloids. Therefore, the authors summarized the separation procedures for mulberry polyhydroxylated alkaloids, including reversed-phase liquid chromatography (RPLC), normal phase liquid chromatography(NPLC), ion exchange chromatography (IEC) and hydrophilic interaction chromatography (HILIC), analyzed their advantages and disadvantages, and proposed the column chromatography method with carbosilane ionic liquid with a new-type adsorbing material. This new-type material is similar with zwitter-ion stationary phase in hydrophilic interaction chromatography but different in the ionic bond connecting zwitterions in the stationary phase, while showing the characteristic zwitter-ion separation, in order to provide reference for separating polyhydroxylated alkaloids from mulberry herbs and developing new-type mulberry drugs, successfully separate polyhydroxylated alkaloid and develop targeted drugs for patients with different types of diabetes. Finally, the authors defined the development and application prospect of polyhydroxylated alkaloids, which can modify the stationary phase in the chromatographic analysis by regulating the ionic liquid.关键词：mulberry;polyhydroxylated alkaloids;ionic liquids;separation;summary
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发布时间：2024-01-04 - 摘要：Because traditional Chinese medicine (TCM) feature complicated active ingredients and diverse compatible herbs, it is difficult to define the active ingredients and their synergistic effect and even conduct a study on the compatibility between single TCM ingredients. The spectrum-effect relationship, as an emerging method, has been gradually applied in studies on the effect material foundation of compounds, component compatibility, processing mechanism, efficiency prediction and process optimization. The study method is a data-based analytical techniques to associate the multidimensional fingerprint data with the pharmacological indexes as well as a crossing field between pharmacy and mathematics. The study relies on the data analysis technique and focuses on selecting objective and reasonable data analysis technique. In this paper, the authors summarized the advance in applications of data analysis technique in the spectrum-effect relationship, including,forecasting the analysis method on the correlation between ingredients and efficacy:grey relational analysis, artificial neural networks, bivariate correlations analysis. Defining the analysis methods on the contribution of each component to the efficacy:multiple linear regression, partial least squares;simplifying the analysis method for finding major active ingredients:principal component analysis, canonical correlation analysis. Besides, the characteristics of currently common data analysis technique, research ideas and their applicable research objects were summarized. At last, the authors discussed the limitations in various data analysis technique, gave suggestions for selecting data analysis technique in the spectrum-effect relationship and recommended the reasonable combination of a variety of data analysis technique, so as to establish an ideal spectrum-effect relationship model and ensure the objectivity and authenticity of the results.关键词：spectrum-effect relationship;data analysis technique;summary
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发布时间：2024-01-04 - 摘要：Polygonatum sibiricum is a traditional Chinese medicine of drug Edible, with many functions of tonifying qi and nourishing yin, strengthening spleen and kidney, moistening lung and so on. It mainly contains polysaccharides, steroidal saponins, anthraquinones, alkaloids, cardiac glycosides, lignin, vitamins and other compounds, and polysaccharide is the main of them. In recent years, the study of composition structure analysis and functional activity of Polygonatum sibiricum polysaccharides (PSP )has made great progress. In this paper, the relevant research of the main chemical composition and molecular weight determination,the structure analysis and functional activity of PSP were systematically summarized by referring to retrieve related documents, the results showed the chemical composed of PSP mainly included arabinose, galactose, glucose, mannose, fructose and galacturonic acid, and small amounts of xylose and glucuronic acid, in terms of pharmacological activity, it has certain anti-tumor antioxidant and anti-aging, enhancing immunity, regulating blood glucose and lipid, anti-inflammatory and regulating cell endocrine effects, and broad application and market prospects. However, the lack of research about PSP chemical composition and biological effects and mechanisms in vivo or vitro, therefore, this article will discuss with the prospect of its application prospects and proposing further research, provide the theoretical basis of health care products and treatment of disease.关键词：polygonatum sibiricum polysaccharides;structure analysis;functional activity
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