最新刊期
卷 23 ， 期 6 ， 2017
- 摘要：Objective: To prepare celastrol-Coicis Semen oil microemulsions (CC-MEs) and evaluate its physicochemical properties and in vitro anticancer activity. Method: The optimal oil phase,emulsifier and co-emulsifier of CC-MEs were selected by examining solubility of celastrol in different media.According to determination of particle size,Zeta potential,morphology,encapsulation efficiency and drug loading,the prescription was optimized.The stability and in vitro release of the microemulsions was investigated.The cytotoxicity of CC-MEs on cervical cancer HeLa cell was studied by MTT assay and its in vitro anti-tumor activity was evaluated. Result: Optimum prescription was as following:celastrol of 10 mg,Coicis Semen oil of 400 mg as material and oil phase,cremophor RH40 of 450 mg and polyethylene glycol 400 (PEG-400) of 150 mg.CC-MEs displayed the spherical surface with mean particle size of (31.63±0.63) nm,polydispersity index (PDI) of 0.06±0.01 and Zeta potential of (-10.14±1.35) mV.The cumulative in vitro release rate of celastrol was (19.89±0.59)% in 48 h with a sustained-release characteristics.MTT experiment showed that 50%inhibitory concentrations (IC50) of CC-MEs were 0.82 μmol·L-1 counted as celastrol and 20.7 mg·L-1 counted as Coicis Semen oil;the combination index (CI) was 0.93,indicating that celastrol and Coicis Semen oil had a synergistic effect. Conclusion: CC-MEs presents small particle size,well-distributed,good stability and less excipients.In addition,preparations with the compatibility of celastrol and Coicis Semen oil can enhance the inhibitory effect on proliferation of HeLa cell.关键词：celastrol;Coicis Semen oil;microemulsions;Hela cell;antitumor activity
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发布时间：2024-01-04 -
Preparation of Scutellarin Sustained-release Microspheres and Study on Its Pharmaceutical Properties
摘要：Objective: To select the best formula and technology for the preparation of scutellarin sustained-release microspheres and study on its pharmaceutical properties. Method: S/O/W emulsification solvent evaporation method was adopted to prepare scutellarin sustained-release microspheres,taking composite score of drug loading,encapsulation efficiency and yield as index,orthogonal test was used to optimize preparation process of scutellarin sustained-release microspheres and its in vitro release was investigated.This microspheres was charaterized in some ways,such as laser particle size analyzer,scanning electron microscopy,Fourier transform infrared spectroscopy and X-ray diffraction. Result: Optimum preparation process was as following:scutellarin dosage of 25 mg,poly(lactide-glycolide acid) (PLGA) amount of 200 mg,polyvinyl alcohol (PVA) amount of 1.0%,dichloromethane-acetone (1.7:0.3),stirring speed of 1 000 r·min-1.The drug loading,encapsulation efficiency and yield were (6.18±0.11)%,(50.79±2.01)% and (91.18±2.19)%,respectively;in vitro cumulative release of 30% needed ≥600 h,average particle size was (126.0±2.10) μm.The surface of scutellarin sustained-release microspheres was round without adhesion.The internal of microspheres had some crystals of scutellarin.FT-IR and XRD indicated that scutellarin still existed in the form of crystals. Conclusion: Sustained-release microspheres of scutellarin is successfully prepared with emulsification solvent evaporation method.This optimized process is simple and reasonable,which is basis for further research of scutellarin preparations.关键词：scutellarin;poly(lactide-glycolide acid);microspheres;solvent evaporation method;polyvinyl alcohol;drug loading;encapsulation efficiency- 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate urine-reducing components in petroleum ether part of salt-processed Alpiniae Oxyphyllae Fructus and verify their pharmacodynamics for clarifying its material basis and mechanism of urine-reducing. Method: HPLC and other chromatographic means were employed to separate and purify medicinal compositions,the structures of these compounds were identified by physicochemical properties and 1H-NMR,13 C-NMR spectral data.Guinea pig bladder detrusor was prepared by isolated tracheal thermostatic perfusion experimental method and isolated in the Kerbs solution.The tension of 7-epi-teucrenone B on the isolated guinea pig bladder detrusor and the effect of 7-epi-teucrenone B on guinea pig bladder detrusor contraction incubation by four antispasmodic agents including acetylcholine,histamine phosphate,BaCl2 and dopamine were measured by BL-420S biological function experiment system. Result: Nine compounds were isolated and identified from petroleum ether part of salt-processed Alpiniae Oxyphyllae Fructus,such as nootkatone,11-hydroxy-valenc-1(10)-en-2-one,teucrenone,7-epi-teucrenone B,12-hydroxynootkatone and so on.By confirmation of pharmacodynamics,7-epi-teucrenone B was closely related to urine-reducing. Conclusion: 7-Epi-teucrenone B inhibited contractile tension of isolated bladder detrusor may be through the antagonizing release of H1 receptor of histamine phosphate,it may be the urine-reducing component in petroleum ether part of salt-processed Alpiniae Oxyphyllae Fructus fruits.关键词：Alpiniae Oxyphyllae Fructus;salt-processed product;medicinal composition;bladder detrusor;7-epi-teucrenone B;urine-reducing;chemical separation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the difference of squeezing juice from Zingiberis Rhizoma Recens(SJZRR),boiling juice from Zingiberis Rhizoma Recens(BJZRR) and Zingiberis Rhizoma(BJZR) in gastrointestinal function. Method: The intestinal propulsion ratio and anti-diarrhea index were applied to observe the effect of different ginger juice on gastrointestinal motility, and the heterogeneous behavior after yielding of kaolin was oberseved to determine the antiemetic effect of these ginger juice. Result: SJZRR,BJZRR and BJZR all had the effects on promoting intestinal propulsion,and BJZRR had the strongest role;but they all had no anti-diarrhea effect.Compared with the model group,the amounts of kaolin in SJZRR,BJZRR and BJZR groups were all decreased,and the BJZRR group showed significant differnce. Conclusion: BJZRR shows the best effects on gastrointestinal function by comparing with SJZRR and BJZR.The results indicate that suitable ginger juice should be chosen for processing Chinese materia medica in order to get a better clinical curative effect.关键词：Zingiberis Rhizoma Recens;squeezing juice;boiling juice;Zingiberis Rhizoma;small intestinal propulsion rate;diarrhea index;kaolin
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发布时间：2024-01-04 - 摘要：Objective: To discuss on feasibility of Halloysitum Rubrum and Limonitum as substitutes of Terra Flava Usta by comparing their pyrolysis characteristics and infrared spectral similarity. Method: Pyrolysis characteristics was analyzed by thermogravimetric differential thermal analysis.Infrared spectrum test was employed potassium bromide tabletting method with the spectral resolution of 4 cm-1 and the measurement range of 4 000-500 cm-1,the data were handled by the Ominc software for automatic baseline correction,smooth and ordinate normalization.Data processing and charting were done with Origin 8.0 software. Result: Pyrolysis characteristics of Halloysitum Rubrum,Limonitum and Terra Flava Usta in 400-600℃ all showed a period of weightlessness steps;however,the pyrolysis characteristics of Halloysitum Rubrum and Limonitum did not show the complexity of pyrolysis characteristics of Terra Flava Usta.Taking Terra Flava Usta as the control,similarity values of Halloysitum Rubrum and Limonitum were relatively low,but similarity values between Limonitum and Terra Flava Usta were at the medium level. Conclusion: In terms of pyrolysis characteristics and infrared spectral similarity,Limonitum is more suitable for a substitute of Terra Flava Usta by comparing with Halloysitum Rubrum.关键词：processing accessories;Terra Flava Usta;Halloysitum Rubrum;Limonitum;pyrolysis characteristics;infrared spectrum
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method of total cholic acid,hyodeoxycholic acid and chenodeoxycholic acid in Arisaema Cum Bile,which could provide an valuable mean to control its quality. Method: The analysis was performed on ultraviolet spectrophotometry to determine the content of total cholic acid in Arisaema Cum Bile with chenodeoxycholic acid as marker substance.HPLC-CAD was used to analyze determination of hyodeoxycholic acid and chenodeoxycholic acid in Arisaema Cum Bile. Result: In the range of 0-2.435 g·L-1,it showed good linear relationships between the concentration of chenodeoxycholic acid and absorbance.The average recovery was 98.98% with RSD of 1.0%.Hyodeoxycholic acid and chenodeoxycholic acid were linear with the peak area in the range of 4.7-94,5.44-108.8 mg·L-1,the average recovery was 99.53% and 99.58%,respectively. Conclusion: This method is simple,accurate and suitable for the quality evaluation of Arisaema Cum Bile.关键词：Arisaema cum Bile;Ultraviolet spectrophotometry;total cholic acid;hyodeoxycholic acid;chenodeoxycholic acid;methodological review
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发布时间：2024-01-04 - 摘要：Objective: To set up the HPLC fingerprint chromatograms analytical methods of different processed products of Armeniacae Semen Amarum with the personality characteristics. Method: 50% Methanol was used for ultrasonic extraction of three processed products of Armeniacae Semen Amarum.HPLC was employed with mobile phase of acetonitrile-0.1% phosphoric acid water solution for gradient elution,the column temperature at 25℃,the detection wavelength at 225 nm,the flow rate of 1.0 mL·min-1.HPLC fingerprint differences of different processed products of Armeniacae Semen Amarum were compared. Result: There were significant differences in fingerprints between crude products and processed products,but fingerprints of blazed processed products and stir-fried products were relatively similar. Conclusion: This estabilished method can be used for determining fingerprint chromatograms of different processed products of Armeniacae Semen Amarum.HPLC fingerprints have changed significantly after being processed.关键词：Armeniacae Semen Amarum;processed products;fingerprint;prunasin;amygdalin;stir-frying;blazed
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发布时间：2024-01-04 - 摘要：Objective: To detect aroma characteristics of Aromi Fructus from different originsby using electronic nose combined with automatic headspace-gas chromatography-mass spectrometry (HS-GC-MS)in two aspects, namely overall aroma profile and specific aroma composition, in order to study material basis of Aromi Fructus's odors. Method: With Aromi Fructus of different varieties and from different origins-Guangdong,Yunnan,Burma,Vietnam and Laos as a research subject,the volatile components were detected by using electronic nose and HS-GC-MS technique.A bioplot was used to analyze the correlation among samples,E-nose gas sensors and chemical volatile compounds. Result: E-nose showed a good differentiation of Aromi Fructus from different origins, with the identification index of 83.9%.Homebred Amomum villosum was classified in class A, and imported Aromi Fructus was classified in class A.A total of 70 volatile components were identified by HS-GC-MS, including terpenes,esters,alcohols,ketone and alkane.Aromi Fructus of different varieties and from different origins have different composition and content, with the same classification with the results of E-nose.A bioplot of PLS displayed the high correlation between sensors s14,s8/s6/s3 and terpenes,esters and alcohols indicating that special sensors were highly correlated with some aroma constituents. Conclusion: Advanced chemometrics and E-nose are used to discriminate Aromi Fructus from different origins.By combining with HS-GC-MS technique, this study provides further experimental evidence for material bases of Aromi Fructus's odors.关键词：aroma characteristics;bioplot;electronic nose;HS-GC-MS;Aromi Fructus
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发布时间：2024-01-04 - 摘要：Objective: To conduct correlation analysis between roots, stems and leaves of Sophora flavescens from different habitats of Guizhou and latitude and longitude, altitude, mean humidity and mean temperature, and investigate the alkaloid contents accumulation in different parts of S. flavescens from different environments. Method: UV spectrophotometry method was applied to detected the contents of alkaloids in roots, stems and leaves of S. flavescens, and the correlation analysis was conducted to study the correlation between cumulative alkaloids difference and ecological factors. Result: The contents of alkaloids in different parts of S. flavescens were in the order of roots > leaves > stems, and the contents were highest in those from Bijie City Dafang County, whose total contents in roots, stems and leaves were 20.45 μg·g-1. The different parts of S. flavescens were differently affected by ecological factors. Conclusion: There were significant differences in alkaloid content of S. flavescens roots, stems, and leaves from different areas. Relative humidity and mean annual temperature among the ecological factors showed greatest impact on alkaloid content. The results can provide a theoretical basis to S. flavescens standardized cultivation and management as well as targeted cultivation, and provide a theoretical basis for S. flavescens high quality production and comprehensive utilization of its non-medicinal parts.关键词：Sophora flavescens;alkaloids;ecological factors;correlation
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发布时间：2024-01-04 - 摘要：Objective: To analyze the genetic relationship and quality differences among different regional Chrysanthemi Flos by evaluating the quality of germplasm resources of Huai Chrysanthemi Flos. Method: The methods of field observation, visiting herbalist were adopted to investigate and analyze the germplasm resources of Huai Chrysanthemi Flos. The contents of chlorogenic acid, luteoloside and 3, 5-O-double coffee acyl quinic acid were determined by the method in the Chinese Pharmacopoeia of 2015. The content of flavonoids was determined by ultraviolet-visible spectrophotometry. The contents of moisture, total ash, acid insoluble ash content, extract, volatile oil were determined by using the method in the Chinese Pharmacopoeia of 2015 to evaluate the quality of medicinal materials from Chrysanthemi Flos of different regions. Random amplified polymorphic DNA (RAPD) molecular marker method was used to analyze the genetic relationship among Chrysanthemi Flos from different regions. Result: By analyzing the six indicatiors of Chrysanthemi Flos, its effective composition all conform the Chinese Pharmacopoeia of 2015. The results show that Wen county and Wuzhi county are suitable for the growth of Chrysanthemi Flos to be original regions. Seven RAPD primers had amplified into 50 clear bands including 46 polymorphic bands, so the polymorphism ratio was 92.00%. Huai Chrysanthemi Flos, Hang Chrysanthemi Flos, Gong Chrysanthemi Flos and Chrysanthemi Indici Flos can't be clustered, not only indicating that the genetic relationship among them was far, but also proving that abundant genetic diversity existed in the Chrysanthemi Flos germplasm resources. Huai Chrysanthemi Flos was clustered into a broad category, showing that the genetic purity of Huai Chrysanthemi Flos was higher. Conclusion: Analysis on Huai Chrysanthemi Flos germplasm resources, not only provided scientific reference basis for the standardization of Huai Chrysanthemi Flos planting, but also offered a scientific foundation for protection of Huai Chrysanthemi Flos resources and reasonable development and utilization of medicinal materials.关键词：Huai Chrysanthemi Flos;germplasm resources;random amplified polymorphic DNA molecular marker
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发布时间：2024-01-04 - 摘要：Objective: To study the separation technology of total flavonoids from leaves of Lonicera japonica and investigate their antibacterial activity in vitro against nine common pathogenic bacteria. Method: The leaves of L. japonica from Henan Fengqiu were used, and the total flavonoids were obtained by macroporous resin column separation as the main investigation indexes. Then their antibacterial activity in vitro were evaluated by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and inhibition zone. Result: A simple and convenient separation technology was established, proving the broad and better antibacterial activity against nine common pathogenic bacteria (MIC values were 1.95-62.5 g·L-1, and MBC values were almost 125 g·L-1). Meanwhile, when the total flavonoids fraction coexisted with plenty of chlorogenic acid (4.2%), its yields (10.5%) and activity were higher. Conclusion: The total flavonoids from the leaves of L. japonica could be developed as the antibacterial agent or feed supplement for livestock and poultry because of their broad and better antibacterial activity, and this study will lay the foundation for the rational development and utilization of the leaves of L. japonica.关键词：leaves of Lonicera japonica;total flavonoids;pathogenic bacteria;antibacterial activity
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发布时间：2024-01-04 - 摘要：Objective: To study on the intraspecific variation of Forsythiae Fructus,clarify the morphological characteristics,quality and yield in different habits and different fruit types, and lay the foundation for better development, utilization and protection of germplasm resources as well as breeding of fine varieties of Forsythiae Fructus. Method: The morphological characteristics of different fruit types were observed, and their length,width and yield were measured as basis for determining the type of fruit.The contents of chemical components in different fruit types were measured by using high performance liquid chromatography (HPLC)and the comprehensive evaluation was then carried out. Result: Upon investigation of intraspecific variation,the fruits of Forsythiae Fructus were classified into long oval, oval, spindle, elongated and round types. The economic indexesand properties of these different fruit types were analyzed and described. The results showed that the yield of spindle shape was highest, so it was among the high yield types.The phillyrin,forsythiaside A, ethanol soluble extraction and weight per 100 fruits in agricultural economic index of different fruit types were measured, and a comprehensive evaluation of different sources of Forsythiae Fructus was carried out by the method of principal component analysis.The comprehensive evaluation of spindle shape was better. Conclusion: This experiment could lay foundation for the breeding of new varieties and the quality improvement of Forsythiae Fructus.关键词：Forsythiae Fructus;fruit types;chemical composition;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Bufonis Venenum. Method: Positive silica gel, glycol silica gel and RP-18, Sephadex LH-20 and other materials were used to isolate and purify the petroleum ether, ethyl acetate, acetone and methanol extracts from Bufonis Venenum, and the structures of the compounds were identified according to their physicochemical properties and 1D (1H, 13C) and 2D-NMR (COSY, NOESY, HSQC, HMBC) spectroscopy analysis. Result: The 12 compounds were identified as:resibufogenins including isobufalin methyl ester (1), cinobufotalin (2), bufalin (3), telocinobufagin (4), bufotalin (5), bufotenidines including 5-hydroxy-N-acetyltryptamine (6), hippophamide (7), and other types including n-pentadecanoic acid (8), 4,4'-diphenylmethane-bis(methyl) carbamate (9), obtucarbamate A (10), di(2-ethylhexyl) phthalate (11), and di(6-ethylhexyl) phthalate (12). Conclusion: Compound 1 was a new natural compound and compounds 6-8, 10 and 12 were obtained from Bufonic Venenum for the first time.关键词：Bufonis Venenum;chemical constituents;structure identification;isobufalin methyl ester
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发布时间：2024-01-04 - 摘要：Objective: To study the correlation between the GC-MS fingerprints of volatile oil from Angelicae Sinensis Radix of different regions and its effect on proliferation. Method: The volatile oil from Angelicae Sinensis Radix was extracted by steam distillation, and GC-MS analysis technology was used to establish the fingerprints of volatile oil from 44 batches of Angelicae Sinensis Radix of different regions. Mass spectrometry database and retention index were used to identify the chemical components indicated in various chromatographic peaks, and then Methyl thiazolyltetazolium(MTT) was used to investigate the effect of volatile oil from Angelicae Sinensis Radix of different regions on HUVECs proliferation. Finally, the fingerprint-effect relationship of volatile oil and promoting HUVECs proliferation effect could be established and analyzed by linear regression analysis. Result: Linear regression analysis showed that, X1 and X2 peaks were negatively correlated with cell proliferation rate, and other peaks showed positive correlation. Among them, X3, X6 and X7 peaks had larger linear regression coefficient, showing main constituents for promoting HUVECs proliferation. Conclusion: This method can quickly and effectively establish the spectrum-effect relationship for volatile oil from Angelicae Sinensis Radix, providing experimental basis for studying their pharmacological properties.关键词：Angelicae Sinensis Radix;volatile oil;HUVECs;fingerprint-effect relationship;linear regression analysis
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发布时间：2024-01-04 - 摘要：Objective: To develop a UPLC method for the determination of linarin luteolin, acacia-7-O-β-D-glucopyranoside and acacetin contents in different parts (stem, leaf, flower and fruit) of Buddleja lindleyana, and compare the distribution of 4 kinds of flavonoids in different parts of B. lindleyana. Method: The optimal conditions of separation and detection were achieved on an Acquity BEH C18 column (2.1 mm×50 mm, 1.7 μm), with acetonitrile (A)-0.1%formic acid solution (B) as the mobile phase for gradient elution, at a flow rate of 0.15 mL·min-1, with a detection wavelength of 330 nm. The column temperature was 30℃ and sample volume was 2 μL. Result: Linarin, luteolin, acacia-7-O-β-D-glucopyranosideand acacetin showed a good linearity in the range of 0.002 0-0.600 3 (r=0.999 7), 0.000 4-0.040 1(r=0.999 9), 0.000 4-0.039 9(r=0.999 9) and 0.000 2-0.020 0 μg (r=0.999 9) respectively. Their average recoveries were 100.2%, 97.1%, 94.3% and 92.4%. Conclusion: The method was accurate and simple with high repeatability and stability, and can be used for the determination of multiple flavonoids in different parts of B. lindleyana.关键词：Buddleja lindleyana;linarin;luteolin;acacia-7-O-β-D-glucopyranoside;acacetin;stem;leaf;flower;fruit
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发布时间：2024-01-04 - 摘要：Objective: To separate and identify flavonoids extraction from Mori Cortex rapidly, and investigate their fragmentation behavior and secondary fragmentation pattern upon electrospray ionization. Method: The extraction was separated and identified inelectrospray ionization(ESI) negative mode by ultra performance liquid chromatography coupled with quadrupoletiem-of-flight mass spectrometry (UPLC-Q-TOF MS) technique.It was conducted on Waters BRH (2.1 mm×50 mm, 1.7 μm) column with 0.1% formic acid solution (phase A) and acetonitrile (phase B) as the mobile phase for gradient elution.The column temperature was 25℃ and the flow rate was 0.3 mL·min-1. Result: Accurate relative molecular mass obtained by UPLC-Q-TOF-MS technique was combined with secondary mass spectrumfragmentation information for data statistics and analysis by using Masslynx 4.1.By referring to SciFinder database, 13 flavonoids, including 7 Diels-Alder compounds (represented by sanggenon C and sanggenon D) and 6 prenyl flavonoids (represented by morusin), were separated and identified from the flavonoids extraction of Mori Cortex. Conclusion: The loss of H2 and H2O was characterized here by using UPLC for the first time and their fragmentation behavior upon electrospray ionization was discussed. providinga rapid, simple and reliable method for the identification of flavonoids extraction from Mori Cortex.关键词：Mori Cortex;total flavonoids;Diels-Alder compounds;prenylated flavonoid;UPLC-Q-TOF-MS
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发布时间：2024-01-04 - 摘要：Objective: To analyze and compare the headspace solid-phase microextraction methods (PA, PDMS, and PDMS-DVB) on adsorption property of volatile compounds in the dried whole plants of Hedyotis diffusa, and then compare them with traditional steam and distillation coupling with gas chromatography-mass spectrometry (SD-GC-MS). Method: Three types of SPME fibers including PDMS, PDMS-DVB and PA as well as steam distillation method were used for extraction, and GS-MS was combined for determination of the contents of volatile compounds. Result: The 50 compounds were identified by using PA method; 51 compounds were identified by using PDMS-DVB; 18 compounds were identified by using PDMS; and 41 components were identified by using SD-GC-MS. As compared with SPME method, PA method had the highest similarity, with 21 common components; followed by PDMS-DVB (15 common components) and PDMS (6 common components); 16 components were not identified by SPME method. Conclusion: HP-SPME-GC-MS is a simple, rapid and effective method for detecting the volatile components in H. diffusa, but cannot completely take the place of SD-GC-MS method.关键词：Hedyotis diffusa;HP-HPME-GS-MS;PA;PDMS-DVB;PDMS;volatile constituents
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发布时间：2024-01-04 - 摘要：Objective: To study flavonoids of Zanthoxyli Pericarpium(ZP) correlated with appearance color and provide a reference for its quality evaluation. Method: Introducing chroma space system CIE L*, a*, b* to quantify of ZP's powder color objectively. Studying its correlation with color values by the indexes of total flavonoids, rutin, hyperoside, quercetin, and hesperidin. Result: Zanthoxylum bungeanum's content of total flavonoids, rutin and quercetin are positive correlated with L* and a* significantly (P<0.05). There is no significant correlation between total flavonoids, lutin and b*. The content of hyperoside and quercetin are positive correlated with b* significantly (P<0.05). Z. schinifolium's content of hesperidin is negative correlated with L*, a* and b* significantly (P<0.05). Conclusion: There is a certain degree of correlation between ZP's total flavonoids, rutin, hyperoside, quercetin, and hesperidin and its color values.关键词：Zanthoxyli Pericarpium;color;correlation;flavonoids
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发布时间：2024-01-04 - 摘要：Objective: To establish a targeted profiling qHNMR (TP-qHNMR) method for content determination of gentiopicroside and loganic acid in 4 kinds of Gentianae Macrophyllae Radix (Gentiana macrophμlla, G. straminea, G. crassicaulis and G. dahurica). Method: TP-qHNMR software of Chenomx NMR suit was employed and standard 1H-NMR spectra of gentiopicroside and loganic acid were collected. Gentianae Macrophyllae Radix was extracted by using methanol ultrasonic method and the dried extraction was reconstituted in CH3OD before spectra were recorded. All spectra were recorded in the same condition, and then targeted profiling quantification was achieved in reference to chemical shape indicator (CSI) by Chenomx profiler and further validated by HPLC-UV method. Result: The established TP-qHNMR method was accurate and reliable for determination of gentiopicroside and loganic acid in Gentianae Macrophyllae Radix, showing no significant difference with HPLC-UV method. The contents of gentiopicroside and loganic acid in 4 kinds of Gentianae Macrophyllae Radix were as follows:G. crassicaulis > G. macrophμlla > G. straminea > G. dahurica. Conclusion: TP-qHNMR was accurate and reproducibly for quality control of Gentianae Macrophyllae Radix, and can be widely applied for quality control of traditional Chinese medicine.关键词：Gentianae Macrophyllae Radix;gentiopicroside;loganic acid;TP-qHNMR;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To investigate the activity change of six kinds of cytochrome P450 (CYP450) subunit in the acute depression model rats induced by reserpine based on cocktail probe substrates method,in order to explore the pathogenesis of the depression from interaction of drug metabolism. Method: Based on the literatures at home and abroad to establish the acute depression model induced by reserpine,rats were randomly divided into the blank group,the model group and the venlafaxine group.Theophylline,chlorzoxazone,tolbutamide,dextromethorphan,omeprazole and midazolam were selected as probe substrates of CYP1A2,CYP2C6,CYP2D1,CYP2D2,CYP2E1 and CYP3A2 in rats,LC-MS/MS was developed for determining concentrations and pharmacokinetic parameters of six kinds of mixed probe in rats. Result: The concentration of tolbutamide in the model group rats increased significantly with slow metabolism,the concentration of midazolam in rats decreased significantly and metabolism accelerated.After administration of venlafaxine,concentrations of theophylline,chlorzoxazone and midazolam in rats increased significantly with slow metabolism. Conclusion: Acute depression model state induced by reserpine has strong inhibition effect on CYP2D1 and CYP2D2 in rats,and has strong induction on CYP3A2.Venlafaxine has strong inhibition effect on CYP1A2,CYP2C6,CYP2E1 and CYP3A2 in the model rats.关键词：depression;reserpine;acute depression model;Cocktail probe method;CYP450 subunit;tinidazole
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of different compatibility on pharmacokinetics in rats of main components in Panacis Majoris Rhizoma from Tengzhu Weikang prescription after oral administration. Method: SD rats were randomly divided into Panacis Majoris Rhizoma (PMR) group,PMR-Actinidia chinensis (AC) group,PMR-Atractylodis Macrocephalae Rhizoma (AMR) group,PMR-Polygonati Rhizoma (PR) group,PMR-Sparganii Rhizoma (SR) group and Tengzhu Weikang prescription group.After oral administration with dosage of 10.0 g·kg-1,HPLC was employed with the mobile phase of acetonitrle-0.02%phosphoric acid aqueous solution for gradient elution and detection wavelength at 205 nm.The pharmacokinetic parameters of ginsenoside Re,ginsenoside Rb1,ginsenoside Ro and chikusetsusaponin Ⅳa were calculated by DAS 3.0 software. Result: Compared with PMR group,most of PMR-AC group,PMR-AMR group,PMR-PR group,PMR-SR group and Tengzhu Weikang prescription group showed a higher Cmax and AUC0-t for ginsenoside Re,ginsenoside Rb1,ginsenoside Ro and chikusetsusaponin Ⅳa,and all showed a lower clearance rate/bioavailability (CL/F).Compared with PMR group,Tengzhu Weikang prescription group showed slower metabolism and higher plasma concentration of ginsenoside Re,ginsenoside Rb1,ginsenoside Ro and chikusetsusaponin Ⅳa. Conclusion: Different compatibility has effect on pharmacokinetic parameters of main efficacy components in PMR from Tengzhu Weikang prescription.关键词：Tengzhu Weikang prescription;Panacis Majoris Rhizoma;Ginsenoside Re;ginsenoside Rb1;ginsenoside Ro;chikusetsusaponin Ⅳa;compatibility;pharmacokinetics
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发布时间：2024-01-04 - 摘要：Objective: To analyze and identify the constituents in rat serum after oral administration of total saponins from Panacis Quinquefolii Radix fruit (TSPQF),and provide a reference for the pharmacodynamics material basis research. Method: The male Wistar rats were selected as experimental animals,rats were intragastric administered TSPQF at a dose of 2.6 g·kg-1.The serum samples were collected and pretreated.The absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified by combination of UPLC-Q-TOF/MS and multivariate statistical analysis. Result: A total of 9 absorbed components were detected and identified,including 5 prototype components,such as pseudoginsenoside F11,ginsenoside Rc,Rb3,Rd and protopanaxatriol.The other 4 metabolites were protopanaxadiol,pseudoginsenoside RT5,ginsenoside CK,Rh2,respectively. Conclusion: These nine constituents absorbed into blood may be the potential bioactive components in TSPQF,this result can provide a scientific basis for clarifying its substance basis of pharmacodynamics.关键词：Panacis Quinquefolii Radix fruit;total saponins;absorbed constituents in rat serum;ginsenoside;UPLC-Q-TOF/MS;intragastric administration
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发布时间：2024-01-04 - 摘要：Objective: To investigate the immunomodulatory effect of oxymatrine on concanavalin A(ConA)-induced liver injury in mice. Method: In the present study, Balb/C mice were randomly divided into five groups:normal group, model group, low and high-dose oxymatrine groups (60, 120 mg·kg-1) and bicyclol group (156 mg·kg-1). The mice in the treated groups were administered with oxymatrine (intraperitoneally) or bicyclol (orally) for 5 consecutive days. 30 min after the last medication, all the mice except the normal group were injected with 15 mg·kg-1 of ConA intravenously to establish liver injury models. 8 hours later, blood samples were taken to test the levels of alanine transaminase(ALT),aspartate aminotransferase(AST), and total bilrubin(TBIL) in serum, and the liver tissues were taken for HE-staining to evaluate the histopathology changes. The expression levels of serum cytokines interferon-γ(IFN-γ),tumour necrosis factor-α(TNF-α),interleukin-4(IL-4),interleukin-6(IL-6), interleukin-10(IL-10) and interleukin-27(IL-27) were measured by luminex. Spleen lymphocytes were isolated and the expression levels of CD4+IL-17A and the percentage of CD4+CD25+FoxP3+Treg were tested by flow cytomerty. Results showed that, after con-A injection, the expression levels of ALT, AST and TBIL were increased (P<0.05, P<0.01) as compared with the normal group. As compared with the model group, bicyclol and oxymatrine (60, 120 mg·kg-1) could markedly decrease the activity of ALT (P<0.05, P<0.01); oxymatrine at 120 mg·kg-1 could significantly decrease the levels of AST (P<0.05), and oxymatrine at both 60, 120 mg·kg-1 could decrease the levels of TBIL (P<0.05). There were no statistical differences in other groups. All treatment groups could relieve the liver pathological changes to different degrees, and reduce the infiltration of inflammatory cells. After treatment with high dose OMT, the levels of IL-10 and IL-27 were significantly increased (P<0.05). Flow cytomerty results revealed that high dose OMT could significantly decrease the expression levels of CD4+IL-17A (P<0.05) and up-regulate the percentage of CD4+CD25+foxP3+Treg cells (P<0.05). Conclusion: oxymatrine could alleviate ConA-induced liver injury by inhibiting the expression of IL-17A and promoting the proliferation of Treg cells.关键词：oxymatrine;concanavalin A;interleukin;Tregs
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发布时间：2024-01-04 - 摘要：Objective: To establish a migration platform for neural stem cells(NSCs) in vitro and verify the applicability of this platform by using Buyang Huanwu Tang, a Chinese classical formula. Method: NSCs were isolated from rat embryos, and used in establishing a migration platform for neural stem cells in vitro by radial cell migration assay, scratch repair dynamic detection, and Transwell chemotaxis system. Then this platform was used to evaluate the effects ofof Buyang Huanwu Tang(300, 600 mg·L-1), Tetramethylpyrazine (10, 50 mg·L-1), as well as stromal-derived factor 1(SDF-1) on NSCs migration. ELISA assay was used to detect the contents of migration regulatory factor SDF-1 and vascular endothelial growth factor(VEGF) in the radial cell migration and transwell system. Result: NSCs migration in varying degrees was observed at different time points in radial migration, scratch repair dynamic detection and transwell chemotaxis system. As compared with the blank group, Buyang Huanwu Tang and Tetramethylpyrazine could significantly promote NSCs migration in radio migration system, but also significantly increase the number of NSCs migrated to lower chamber in Transwell system (P<0.05, P<0.01) in a dose-dependent manner. Additionally, Buyang Huanwu Tang and Tetramethylpyrazine could promote the expression levels of SDF-1 and VEGF in NSCs culture supernatant (P<0.01), where the up-regulation effect on SDF-1 was higher than that on VEGF. We also found that the effect of Buyang Huanwu Tang (600 mg·L-1) and Tetramethylpyrazine (10, 50 mg·L-1) were inhibited after AMD3100 pretreatment, where the number of NSCs migrated to lower champ was reduced significantly. Conclusion: The NSCs migration research platform established in this study is dynamic and multi-dimensional. It can simulate different clinical pathophysiological processes, and in addition, this platform is economical, practical and easy to operate.关键词：Buyang Huanwu Tang;neural stem cells;migration;stromal-derived factor 1;vascular endothelial growth factor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the regulatory effect of the water extract from Mori Folium on the gene expressions of Toll-like receptors (TLRs) in the liver from diabetic mice, and explore its action mechanism. Method: ICR mice were randomly divided into normal group (n=6) and diabetic group. The mice in diabetic group received high-fat and high-sugar fodder for 10 weeks and injection with 100 mg·kg-1 streptozotocin (STZ) for 3 days continuously to establish the diabetic mice models. The successfully modeled mice were then randomly divided into model group, metformin gorup (600 mg·kg-1), Mori Folium groups (high dose 8 g·kg-1, middle dose 4 g·kg-1, and low dose 2 g·kg-1) for 10 weeks, n=6 in each group. The drugs were given by ig administration, and the fasting blood glucose was detected every week. Oral glucose tolerance test was conducted in the mid and later phases of experiment. After ig administration for 10 weeks, serum insulin (INS), total cholesterol (T-CHO), triglyceride (TG) and tumor necrosis factor-α (TNF-α) were detected, and the expression level of TLR 1-9 in the liver was measured with quantitative real-time PCR. Result: After the diabetic model mice were treated with Mori Folium water extract, their water intake was reduced; fasting blood glucose was significantly reduced; glucose tolerance was improved; blood fat was significantly reduced; insulin concentration was dramatically increased; insulin resistance was improved; and serum TNF-α level was reduced (P<0.05, P<0.01). Quantitative Real-time PCR data showed that TLR1, TLR2, TLR3, TLR7, TLR8 and TLR9 mRNA expression levels were significantly increased in livers of the diabetic rats (P<0.05, P<0.01, as compared with normal group), and TLR5 expression level in model group was significantly lower than that in normal group. In addition, the expression levels of TLR7, TLR8 and TLR9 in liver of Mori Folium middle dose group were significantly lower than those in model group (P<0.05, P<0.01). Conclusion: The water extract of Mori Folium could ameliorate diabetes, and its mechanism may be partly related to inhibiting the TLRs expression in the liver.关键词：diabetes;water extract of Mori Folium;Toll-like receptors (TLRs);tumor necrosis factor-α (TNF-α)
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发布时间：2024-01-04 - 摘要：Objective: To investigate the molecular targets of Modified GanDou decoction (MGDD) treatment in Cyt C/Caspase signaling pathway in neurons of TX neonatal mice, and explore its corresponding mechanism on regulating Cyt C/Caspase signaling pathway. Method: neurons of TX neonatal mice were obtained by using primary culture method, and were divided into normal group (normal DL neonatal mice neurons, cultured by complete medium), model group (TX neonatal mice neurons, cultured by 10% blank rabbit serum), MGDD group (TX neonatal mice neurons, continuously cultured by rabbit serum containing 5%, 10%, 15% and 20% MGDD), and Butylphthalide group (TX neonatal mice neurons, cultured by rabbit serum containing 10% Butylphthalide). Atomic absorption method was used to detect the effect of different concentrations of MGDD rabbit serum on microelement in the neurons of TX neonatal mice after 24 h treatment flow cytometry was used to analyze the expression of reactive oxygen (ROS) in the neurons of TX neonatal mice after treatment with rabbit serum containing MGDD; Western blot was used to detect the protein expression levels of Cyt C, Caspase-9 and Caspase-3 in the neurons of TX neonatal mice after treatment with rabbit serum containing MGDD. Result: As compared with the model group, MGDD could reduce the contents of copper and iron in the neurons of TX neonatal mice, and increase the content of zinc in a dose-dependent manner (P<0.01). Flow cytometry results showed that MGDD can significantly reduce the release of ROS as compared with model group (P<0.01). Western blot results indicated that MGDD significantly decreased the protein expression levels of Cyt C, Caspase-9, and Caspase-3 in the neurons of TX neonatal mice as compared with the model group (P<0.01). Conclusion: MGDD could relieve the high copper-induced neurons damage, probably by promoting the copper excretion and inhibiting the expression levels of Cyt C, Caspase-9 and Caspase-3 in TX neonatal mice neurons. MGDD achieves the treatment effect by reducing copper content in brain and regulating Cyt C/Caspase signaling pathway.关键词：Wilson's disease;modified Gandou decoction;Cyt C/Caspase signaling pathway;neuron
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of polyphyllin I(PPI) on the cell cycle of human colon cancer cell line HCT 116, and investigate the mechanism. Method: 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to detect the inhibitory activity of PPI on HCT116 cells in vitro, then effect of PPI on the number of HCT116 cellular nuclear was observed by Hoechst 33258 staining; flow cytometry was used to detect the cell cycle changes of HCT116 cells. Western blot assay was used to detect the expression and activity of cell division cyclin2 (CDC2), cell division cyclin25 homolog C (cdc25c) protein in cells; and immunofluorescence and confocal laser scanning microscope were used to detect the effect of PPI on cell microtubules of HCT116 cells. Result: MTT assay showed that PPI inhibited the proliferation of HCT116 in a dose-time dependent manner. Hoechst 33258 nuclear staining showed that there the number of dual-core cells in PPI group was significantly increased(P<0.05,P<0.01); flow cytometry showed that the cell cycle was arrested in G2/M phase. Western blot assay indicated that PPI could not inhibit but could promote the expression and activity of the G2/M phase related proteins CDC2 and CDC25C. In addition, in confocal laser scanning microscope detection, we found that PPI could lead to cell microtubules disorder. Conclusion: PPI could make the HCT116 cells arrested in G2/M phase, and the action mechanism is related to interference with microtubule structure.关键词：polyphyllin I;HCT 116;G2/M phase arrest;microtubule
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-tumor effect of astragaloside combined with curcumin on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis and discuss whether the combination of astragalosideand curcumin has the synergies effect against tumor. Method: HO-8910 ovarian carcinoma animal models withfluorescent protein transfectionwere selected,including G1 model group, G2 Cisplatin group, G3 Astragaloside group, G4 curcumin group, G5 Astragaloside+curcumin group, n=8 in each group. The tumor was weighted and the inhibitory rate was calculated. The protein expression levelsof matrix metallopeptidase 2 (MMP2) and apoptosis regulator (Bcl-2) were detected by immunohistochemical method, and thegene expression levels of MMP2, Bcl-2, miR21, miR15a and miR200a were detected by Real-time PCR. Result: Tumor weights of the tumor-bearing nude mice after treatment were lower than those in model group; the tumor weight in Astragaloside+curcumin group was significantly lower than that in other groups(P<0.05).Immunohistochemical results showed that the levels of the protein expressions of MMP2,Bcl-2 in G2, G3 and G4 groups were lower than those in model group, and the levels in G5 group were significantly reduced (P<0.05). Real-time PCR results showed that the gene expression levels of MMP2, Bcl-2 and miR21 in Chinese medicine groups were lower than those in model group, and the levels in G5 groupweresignificantly reduced (P<0.05); the gene expression levels of miR15a, miR200a were higher than those in model group, and the levels in G5 group were significantly increased (P<0.05). Conclusion: The combination of astragaloside and curcumin has the suppression effect on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis, which may be associated with the down-regulation of MMP2, Bcl-2 and miR21 expressions and the up-regulation of miR15a and miR200a expressions. Therefore, the combination of astragaloside and curcumin has the synergies effect against tumor.关键词：astragaloside;curcumin;ovarian cancer;apoptosis regulator (Bcl-2);matrix metallopeptidase 2 (MMP2)
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protection effect and mechanism of Shengjiangsan on learning and memory abilities of vascular dementia (VD) rats. Method: SD rats were randomly divided into normal group, sham-operation group, model group, positive drug group (donepezil hydrochloride, 1 mg·kg-1), Shengjiangsan low, middle and high dose groups (0.67,1.33,2.66 g·kg-1). VD models were reproduced by improved two vessel occlusion method (2VO), and the intragastric administration of the drugs was started 1 week after surgery, for 8 consecutive 8 weeks. The effect of Shengjiangsan on learning and memory abilities of VD rats, as well as the orientation navigation and space exploration behaviors were observed by using 2VO method. HE staining was used to investigate the pathomorphology in hippocampus; Western blot and immunofluorescence assays were used to explore the expression levels of VEGF, Nestin, and key proteins (p-LRP, p-GSK3β, LRP, GSK3β and β-catenin) in hippocampus. Result: As compared with the model group, all doses of Shengjiangsan could prolong the latency of VD rats in passive avoidance test, shorten the latency of the orientation navigation and space exploration in Morris water maze test and promote the repair of pathological injury in some degree in a dose-dependent manner. In addition, Shengjiangsan up-regulated the protein expression levels of VEGF, Nestin, p-LRP, p-GSK3β and β-catenin in hippocampus in a dose-dependent manner (P<0.05, P<0.01). Conclusion: Shengjiangsan could improve the behaviors of VD rats in learning and memory as well as spatial recognition by activating Wnt signal pathway.关键词：Shengjiangsan;vascular dementia (VD);learning and memory;nerve injury;Wnt parthway
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发布时间：2024-01-04 - 摘要：Objective: To study the regulation effect of Ganle granules(GLG) on transforming growth factor(TGF-β1)/Smad signaling pathways in rats with hepatic fibrosis, and to explore its possible mechanisms for prevention and treatment of hepatic fibrosis. Method: The SD rats were randomly divided into normal group, model group, GLG (1.5, 3.0, 6.0 g·kg-1) groups and colchicine (Col, 0.1 mg·kg-1) group. The hepatic fibrosis rat models were induced by subcutaneous injection of CCl4(50%) twice each week for 12 weeks continuously. Then different doses of GLG (1.5, 3.0, 6.0 g·kg-1) and colchicines (0.1 g·kg-1) were administrated via gavage once a day for 6 weeks from week 7 after modeling. 8 h after the last administration, rats were sacrificed, and their liver samples were taken to observe the pathological and histological degree of liver fibrosis by HE and Masson. Moreover, the protein expression levels of CollagenⅠ, TGF-β1 and Smad2 or TβRⅠ in the liver tissues were determined by immunohistochemistry and Western blot. The mRNA expression levels of CollagenⅠ, Smad2, and TβRⅠin liver tissues were detected by the real-time fluorescent quantitative PCR. Result: As compared with the normal group, the pathological and histological degree of liver fibrosis, as well as protein and mRNA expression levels of Collagen I, TGF-β1, Smad2, and TβR I in model group were obviously increased in model group (P<0.01). As compared with model group, GLG middle and high doses not only obviously improved histopathological changes, but also effectively reduced the protein and/or mRNA expression levels of Collagen I, TGF-β1, Smad2, and TβR I (P<0.05, P<0.01). Conclusion: GLG has protective effect on liver fibrosis rats and its mechanism may be related to regulating the TGF-β1/Smad signaling pathways, inhibiting HSC activation, and reducing excessive extracellular matrix deposition.关键词：Ganle granules;liver fibrosis;TGF-β1/Smad signaling pathway
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Shennao Fuyuan decoction in treatment of ischemic stroke andobserve its effect on hemorheology. Method: According to the diagnostic criteria and inclusion criteria, 96 patients were divided into experimental group and control group with random number table. Patients in control group received conventional western medicine treatment, and patients in experimental group received Shennao Fuyuan decoction on the basis of treatment in control group. Chinese stroke scale(CSS) scores,activities of daily living(ADL) scores, traditional Chinese medicine(TCM) symptom scale scores, clinical efficacy and hemorheology indexes were observed before treatment, 2 weeks and 4 weeks after treatment. Result: After treatment for 2 and 4 weeks, CSS scores, TCM symptom scale scores and hemorheology indexes in experimental group were obviously better than those in control group (P<0.01). After treatment for 2 weeks, ADL scores in treatment group were increased and significantly higher than those in control group(P<0.05), and were extremely significantly higher than control group after treatment for 4 weeks (P<0.01). Clinical efficacy in experimental group was significantly better than that in control group after treatment(P<0.01), and there were no statistical differences in safety indexes between the parameters before and after treatment. Conclusion: Shennao Fuyuan decoction can significantly improve the neurological function, the ability of daily living and clinical efficacy, reduce blood viscosity, and enhance their blood fluidity with high safety in patients with ischemic stroke.关键词：Shennao Fuyuan decoction;ischemic stroke;clinical efficacy;hemorheology;Chinese stroke scale(CSS) scores;activities of daily living(ADL)
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of method of Supplementing Qi, Resolving Phlegm and Promoting Blood Circulation on glucose and lipid metabolism and obestatin in obesity type 2 diabetes mellitus, and investigate its action mechanism. Method: The 86 cases of overweight/obese patients with type 2 diabetes were randomly divided into treatment group and control group, each consisting of 43 cases. Patients in treatment group received traditional Chinese medicine (supplementing Qi, resolving phlegm and activating blood circulation)+basic therapy, and patients in control group received placebo+basic therapy. Both before and after treatment, the fasting blood glucose, postprandial 2 h plasma glucose, fastinginsulin, glycosylated hemoglobin, blood lipids, obestatin, safety indexes (three routine tests, liver and kidney function) were detected; the insulin resistance indexes (HOMA-IR)were calculated, and changes in symptoms, signs and drug adverse reactions were observed in detail. Result: The curative effect of traditional Chinese medicine in treatment group was better than that in control group after treatment(P<0.05), and it can significantly improve the shortness of breath and fatigue/lazy words, drying mouth and polydipsia, limbs numbness or tingling, and other symptoms. After treatment, the body mass index, 2 h postprandial blood glucose, glycosylated hemoglobin, and insulin resistance index in treatment group were lower than those in control group(P<0.05), while the level of obestatin was higher than that in control group (P<0.05); the levels of cholesterol and low density lipoprotein in treatment group were significantly lower than those in control group (P<0.01), while the level of high density lipoprotein was significantly higher than that in control group(P<0.01); there were no statistically significant differences in fasting blood glucose and triglyceride between two groups after the treatment. Conclusion: The method of Supplementing Qi, Resolving Phlegm and Promoting Blood Circulation can improve glucose and lipid metabolism disorder, insulin resistance and obestatin level in treatment group, which may be one of the active ways for Chinese herbal compound of supplementing Qi, resolving phlegm and activating blood circulation.关键词：overweight/obese type 2 diabetes mellitus;method of Supplementing Qi, Resolving Phlegm and Promoting Blood Circulation;traditional Chinese medicine therapy;Obestatin
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Duhuo Jisheng Tang for wind-cold-damp retention type arthritis, and investigate its effects onsynovial fluid inflammatory factors, oxidative stress and adipokines. Method: The 124 patients diagnosed of wind-cold-damp retention type arthritis in Dongguan Hospital of Traditional Chinese Medicine were selected and randomly divided into control group(62 cases) and observation group (62 cases).Patients in control group received conventional therapy, and patients in observation group also received Duhuo Jisheng Tang based on treatment in control group. Clinical symptom scores, synovial fluid indexes, and adipokines in both groups were observed and recorded; the clinical efficacy and complications were compared between two groups after treatment. Result: The effective rate in control group was lower than that in observation group (P<0.05). As compared with the levels before treatment, Visual analogue scale(VAS) and Western Ontario and McMaster Universities Osteoarthritis (WOMAC) scores were decreased. Lyshom scores were increased; tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukine-1β(IL-1β) in synovial fluid were decreased; nitric oxide(NO), malondialdehyde (MDA) levels in synovial fluid were decreased; superoxide dismutase(SOD) and adiponectin(APN) levels were increased. Leptin and visfatin levels were decreased after treatment in both groups, all with significant difference (P<0.05).As compared with the control group, VAS and WOMAC scale scores were lower in observation group after treatment, Lyshom score was higher; synovial fluid TNF-α, IL-6 and IL-1β levels were lower; NO and MDA levels were lower. SOD level was higher. APN level was higher, and the levels of leptin and visfatin were lower, all with significant difference(P<0.05). Conclusion: Duhuo Jisheng Tang is very effective for wind-cold-dampretention type arthritis, and its mechanism may be associated with reducing the levels of synovial fluid inflammatory factors and oxidative stress.关键词：Duhuo Jisheng Tang;wind-cold-dampretention type arthritis;inflammatory factors;oxidative stress;adipokines
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Busui Shengxue decoction on bone marrow stromal cell related cytokines in patients with chronic aplastic anemia(CAA), and discuss its mechanism of action. Method: The 112 cases of CAA patients from March 2012 to October 2015 were randomly divided into control group (56 cases) and observation group(56 cases). Patients in control group received Stanozolol and ciclosporin, and patients in observation group received Busui Shengxue decoction on the basis of treatment in control group. The treatment effects of two groups were observed, and the clinical symptom scores, hemoglobin (Hb), white blood cell count (WBC), platelet count (PLT), bone marrow proliferation degree percentage, the percentage of hematopoietic cells, alkaline fibroblast growth factor (bFGF) and bFGF receptors (bFGFR) mRNA expression levels before and after treatment in two groups were compared. Result: The total effective rate was 87.50%in observation group, higher than 67.86%in control group (χ2=6.231, P<0.05). After treatment, the clinical symptom scores were decreased in both groups, and the scores in observation group were lower than those in control group(P<0.05). After treatment, WBC, PLT, and Hb levels were increased in observation group, and those levels were higher than those in control group (P<0.05). After treatment, the bone marrow hyperplasia degree and percentage in observation group were improved and better than those in control group(P<0.05); hematopoietic cells percentage in observation group (41.68±7.85)%, was significantly lower than that of control group (53.45±8.78)%(P<0.05); bFGF and bFGFR expression levels were increased in both groups, and the levels in observation group were significantly higher than those in control group (P<0.05). Conclusion: Busui Shengxue decoction has better clinical efficacy in treatment of patients with chronic aplastic anemia, which can improve bone marrow hematopoietic function, and the mechanism may be associated with regulating the mRNA expression levels of bFGF and bFGFR.关键词：chronic aplastic anemia;Busui Shengxue decoction;bone marrow stromal cells related cytokines;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To analyze and summarize composition principles of the gynecological Zhengjia prescriptions in ancient medical books by using the method of data mining, in order to provide reference for clinical treatment. Method: The information of gynecological Zhengjia prescriptions collected in the book of Traditional Chinese Medical Classics was retrieved. The composition principles were analyzed using revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering in the Traditional Chinese Medicine Inheritance Support System after data entry. Result: A total of 108 prescriptions were included, involving 188 herbs, including 36 highly used herbs with the use frequency of more than 8 times, 47 commonly used two-herb combinations, 14 three-herb combinations, four core combinations after evolution and 2 new prescriptions. Conclusion: The frequently used herbs in ancient gynecological Zhengjia prescriptions mostly have the effects of blood-enriching, blood circulation promotion, blood-breaking, Yang-warming, Qi regulating and purgation. The core prescriptions are Siwutang and modified Guizhi Fuling Wan.关键词：traditional Chinese medicine inheritance support system;gynecological Zhengjia;date mining;composition principle
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发布时间：2024-01-04 - 摘要：Objective: To analyze composition principles of kidney-tonifying Chinese medicine prescriptions(KTCMP) in Chinese Pharmacopoeia. Method: Traditional Chinese medicine(TCM) prescriptions for tonifying kidney were collected from the 2015 edition of Chinese Pharmacopoeia. We used TCM inheritance auxiliary system V2.5 to analyze the principles of prescription composition. Result: Soreness and weakness of waist and knees, dizziness andtinnitus are common symptoms of kidney deficiency. KTCMP mainly involes 52 syndromes, with liver and kidney deficiency, spleen and kidney deficiency and deficiency of liver-Yin and kidney-Yin as common syndromes. The 142 KTCMPs contain 290 herbs, and Rehmanniae Radix Praeparata, Poria and Angelicae Sinensis Radix were frequently used. Rehmanniae Radix Praeparata-Poria-Dioscoreae Rhizoma-Angelicae Sinensis Radix is the main core combination. As forliver and kidney deficiency syndrome, the drug combination of Liuwei Dihuangwan and Siwutang could be used and combined with Polygoni Multiflori Radix and Lycii Fructus. As for spleen and kidney deficiency, Sijuntang could be used and combined with Lycii Fructus and Dioscoreae Rhizoma, which was derived from Lingzhu Tusi Wan. Conclusion: Based on the relationship between kidney and other viscera, considerations are given to both deficiency and excess. With kidney tonifying as the core, emphasis was given to pathological changes in kidney deficiency, characteristics of Yin and Yang of kidney and combination of reinforcing and reducing methods.关键词：kidney deficiency;kidney tonifying;Chinese patent medicine;Chinese pharmacopoeia
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发布时间：2024-01-04 - 摘要：Objective: To investigate the prescription rules of professor JIANG Liang-duo for treatment of chronic hepatitis B, explore the new prescriptions for chronic hepatitis B, and provide new thought on its prevention and treatment. Method: Retrospective research was used to collect professor JIANG's outpatient prescriptions for the treatment of chronic hepatitis B and establish a relevant database. Then the professor JIANG's prescription rules for the treatment of this disease were explored through Traditional Chinese Medicine Inheritance Support Platform(TCMISP). Result: A total of 160 prescriptions for chronic hepatitis B were screened, and the results showed 61 kinds of common herbs in professor JIANG Liang-duo's prescriptions for the disease, including Paeoniae Rubra Radix, Paeoniae Alba Radix, Bitter Orange, Malt, et al. A total of 116 core combinations were obtained, including 43 pair herbal combinations, 46 triangle herbal combinations, and 27 combinations with four or more herbs. At the same time, evolutionary method was used to achieve 12 new prescriptions for the treatment of chronic hepatitis B. Conclusion: Based on TCMISP, the data mining methods such as text mining and association rules better reflected the prescription rules of JIANG Liang-duo for treatment of chronic hepatitis B, namely:good at using triangle herbal combinations, with lighter and thinner drugs, and mainly based on liver softening and soothing principles to propose differentiation treatment from the state, and pay attention to strengthening the body resistance and eliminating pathogenic factors.关键词：Traditional Chinese Medicine Inheritance Support Platform;prescription rules of professor JIANG Liang-duo;chronic hepatitis B
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发布时间：2024-01-04 - 摘要：Flavonoids are important ingredients in many anti-tumor prescriptions in traditional Chinese medicine(TCM).With the deeper investigation of Chinese herbs' anti-tumor function,and anti-tumor drug development turning from cytotoxic regents towards cell specific signaling targets, flavonoids have gained more and more attentions in drug developing fields because of their high anti-tumor activity and low-toxicity characteristics. This paper reviewed the molecular targets of natural flavonoids that were studied in recent years in the fields of cell proliferation, agiogenesis, invasion and metastasis, apoptosis, DNA and chromosome regulations, as well as multi-drug resistance production. This will help in searching anti-tumor lead compounds among natural flavonoids, and provide reference for screening and structural modification of multi-targeting anti-tumor drugs as well as the polypharmacology research. In addition, the latest achievements and technologies in modern molecular biology were used to reveal the molecular mechanism of TCM anti-tumor and prescription basis, providing a revolution way for TCM modernization. Some suggestions of anti-tumor research on natural flavonoids in the future were also given in the present review.关键词：flavonoids;anti-tumor;molecular target;research progress
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发布时间：2024-01-04 - 摘要：Property, performance and application regularity of traditional Chinese medicine (TCM) is an important part of TCM theory. During explorations for TCM property theory in all aspects and at different levels in recent years, some experimental studies have made a progress in key elements of properties of a specific herb. Heat-clearing herbs are cold in nature, with efficiencies of clearing heat, resolving toxin, draining fire, cooling blood, drying dampness, clearing deficiency heat, and mainly used to treat such interior heat symptoms as exogenous evil invasion and transformation into heat, direct attack of pathogenic heat, hyperactivity of fire due to Yin deficiency, with main symptoms of fever, red tongue and rapid pulse, particularly damp-heat diarrhea and dysentery, jaundice, welling-abscess swellings and sores, heat toxin with skin eruption, carbuncle toxin and hectic fever due to Yin deficiency. With a high value of clinical application, it can be used to treat many infectious diseases and non-infectious diseases in modern times. Due to its cold property, it is commonly used as a representative herb in TCM property theories and experimental studies. In this paper, we summarized all of studies for property theory of TCM about heat-clearing herbs (four Qi, five flavors, channel entry and toxicity) in recent years, in the hope to reveal the progress of studies for properties and mechanisms of heat-clearing herbs, systematically summarize study methods and results, provide enlightenment and reference for further studies and advance a new leap in modern studies for TCM theory.关键词：heat-clearing herbs;property theory of traditional Chinese medicine;modern study;four Qi;five flavors;channel entry;toxicity
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发布时间：2024-01-04
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