最新刊期
      卷 16 ， 期 1 ， 2010
      • XU Ping,GUO Li-wei~,HAN Zhi-feng
        Vol. 16, Issue 1, Pages: 1-4(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.042
        摘要：Objective:To investigate Chinese medicine oil-bearing water bodies ultrafiltrate physical and chemical parameters associated with the process of reverse osmosis membrane.Methods: Looking on oil-bearing water bodies ultrafiltrate of Chinese medicine volatile oil as the research object,determinate the physical and chemical parameters,the process of reverse osmosis membrane flux with time,through data mining technology,explorate the relevance of physical and chemical parameters and membrane process.Results: For Chinese medicine oil-bearing water bodies,the main factors that affecting the stability of composite reverse osmosis membrane flux are Turbidity and pH;the main factors that affecting composite reverse osmosis membrane flux decline rate are Conductivity and pH.Conclusion: Chinese medicine oil-bearing water bodiesultrafiltrate physical and chemical properties,especially pH,is an important factor in the membrane process.  
        关键词：ultrafiltration;physical and chemical parameters;reverse osmosis concentrate;data mining;relevance   
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        发布时间：2024-09-23
      • XU Ping,GUO Li-wei,HAN Zhi-feng
        Vol. 16, Issue 1, Pages: 1-4(2010)
        摘要：Objective: To investigate Chinese medicine oil-bearing water bodies ultrafiltrate physical and chemical parameters associated with the process of reverse osmosis membrane. Methods: Looking on oil-bearing water bodies ultrafiltrate of Chinese medicine volatile oil as the research object, determinate the physical and chemical parameters, the process of reverse osmosis membrane flux with time, through data mining technology, explorate the relevance of physical and chemical parameters and membrane process. Results: For Chinese medicine oil-bearing water bodies, the main factors that affecting the stability of composite reverse osmosis membrane flux are Turbidity and pH; the main factors that affecting composite reverse osmosis membrane flux decline rate are Conductivity and pH. Conclusion: Chinese medicine oil-bearing water bodiesultrafiltrate physical and chemical properties, especially pH, is an important factor in the membrane process.  
        关键词：ultrafiltration;physical and chemical parameters;reverse osmosis concentrate;data mining;relevance   
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        发布时间：2024-09-23
      • LIU Qian~1,LIU Yong-gang~2,LA Xiao-jin~1,JIANG Chun-hua~1,LA Wan-ying~
        Vol. 16, Issue 1, Pages: 4-6(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.006
        摘要：Objective:To establish the optimal extraction technology of fufangqiancao capsules.Methods:The content of mollugin and dry extractive material taken were set as indexes for Orthogonal test.Results: The optimum extraction conditions were 10 fold of 90% alcohol marinating 0.5 h,decocting 2 times and 2h each time.Conclusion: The optimum technology of fufangqiancao capsules is simple,stable and effective.  
        关键词：fufangqiancao capsules;orthogonal test;extraction technology   
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        发布时间：2024-09-23
      • LIU Qian,LIU Yong-gang,LA Xiao-jin,JIANG Chun-hua,LA Wan-ying
        Vol. 16, Issue 1, Pages: 4-6(2010)
        摘要：Objective :To establish the optimal extraction technology of fufangqiancao capsules. Methods :The content of mollugin and dry extractive material taken were set as indexes for Orthogonal test. Results : The optimum extraction conditions were 10 fold of 90% alcohol marinating 0.5 h, decocting 2 times and 2h each time. Conclusion : The optimum technology of fufangqiancao capsules is simple, stable and effective.  
        关键词：fufangqiancao capsules;orthogonal test;extraction technology   
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        发布时间：2024-09-23
      • Vol. 16, Issue 1, Pages: 6-8(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.007
        摘要：目的:优化紫草多糖的提取工艺,探测其分子量组成。方法:采用正交实验方法,以总糖含量为评价指标,对紫草多糖提取工艺进行筛选;将提取得到的紫草多糖进行纯化分离,经体外药效学实验表明其中一组分有抗人乳头瘤病毒（HPV）的作用,用高效凝胶渗透色谱方法（HP-GPC）测定该组分分子量的组成。结果:综合考虑,最佳提取工艺为煎煮3次,加水15倍量,煎煮时间1 h;该多糖的重均分子量分别为28 746,4 877,数均分子量分别为27 336,1 152。结论:优选得到的工艺经济、简单、稳定、可行。  
        关键词：紫草多糖;正交设计;高效凝胶渗透色谱方法;提取   
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      • Vol. 16, Issue 1, Pages: 9-11(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.008
        摘要：目的:优选参芍盆炎净合剂的提取工艺;方法:以芍药苷的提取转移率为考察指标,通过正交试验L9（34）对参芍盆炎净合剂的提取工艺进行优选;结果:最佳工艺为加10倍量水,提取3次,每次1 h。  
        关键词：参芍盆炎净合剂;芍药苷;正交试验   
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        发布时间：2024-09-23
      • ZHU Wen-ying,WANG Yu-rong~
        Vol. 16, Issue 1, Pages: 11-13(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.001
        摘要：Objective:To investigate the preparation process of Hulusu Pills.Methods:To study the best preparation technology by Orthogonal experiment.Results:The appearance and the dissolvability of the pills produced by the best preparation conditions（PEG-4000,materials temperature（95±2）℃,condensate temperature 0～（4 ℃,）inner diameter 2.0 mm）are were selected.Conclusion:The performance of the selected preparation technology is easy,the maneuverability and the repeatability.  
        关键词：Hulusu Pills;preparation;orthogonal experiment   
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        发布时间：2024-09-23
      • ZHU Wen-ying,WANG Yu-rong
        Vol. 16, Issue 1, Pages: 11-13(2010)
        摘要：Objective :To investigate the preparation process of Hulusu Pills. Methods: To study the best preparation technology by Orthogonal experiment. Results: The appearance and the dissolvability of the pills produced by the best preparation conditions(PEG-4000,materials temperature (95±2)℃,condensate temperature 0~4 ℃,inner diameter 2.0 mm)are were selected. Conclusion: The performance of the selected preparation technology is easy,the maneuverability and the repeatability.  
        关键词：Hulusu Pills;preparation;orthogonal experiment   
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        发布时间：2024-09-23
      • LI Xiao-lin,SHAO Ai-juan,CHEN Min,HUANG Lu-qi~
        Vol. 16, Issue 1, Pages: 14-17(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.009
        摘要：Objective:To compare seven protein extraction protocols（PVP buffer,HEPES buffer,Lysis buffer A extraction and TCA/acetone,tris-base/acetone,phenol/ammonium acetate,acetone/phenol/ammonium acetate precipitation） and establish a suitable one for total proteins extraction from Semen Seu Fructus Physalis.Methods:The protein yield,protein purity and SDS-PAGE patterns was determined and analyzed.Results:HEPES buffer extraction produce the purer sample,much more clear bands and highest resolution.PVP buffer and Lysis buffer A extraction had most impurities.TCA/acetone,tris-base/acetone,phenol/ammonium acetate,acetone/phenol/ ammonium acetate precipitation methods had the purest sample,but protein yield and band numbers were lowest.Besides,the methods employing Lysis buffer was easy to produce high-abundance proteins and interfered in the electrophoresis resolution.Conclusion:HEPES buffer extraction was the best sample preparation protocol for SDS-PAGE and 2D PAGE.  
        关键词：Semen Seu Fructus Physalis;protein extraction;SDS-PAGE   
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        发布时间：2024-09-23
      • LI Xiao-lin,SHAO Ai-juan,CHEN Min,HUANG Lu-qi
        Vol. 16, Issue 1, Pages: 14-17(2010)
        摘要：Objective: To compare seven protein extraction protocols(PVP buffer, HEPES buffer, Lysis buffer A extraction and TCA/acetone, tris-base/acetone, phenol/ammonium acetate, acetone/phenol/ammonium acetate precipitation) and establish a suitable one for total proteins extraction from Semen Seu Fructus Physalis. Methods: The protein yield, protein purity and SDS-PAGE patterns was determined and analyzed. Results: HEPES buffer extraction produce the purer sample, much more clear bands and highest resolution. PVP buffer and Lysis buffer A extraction had most impurities. TCA/acetone, tris-base/acetone, phenol/ammonium acetate, acetone/phenol/ ammonium acetate precipitation methods had the purest sample, but protein yield and band numbers were lowest. Besides, the methods employing Lysis buffer was easy to produce high-abundance proteins and interfered in the electrophoresis resolution. Conclusion: HEPES buffer extraction was the best sample preparation protocol for SDS-PAGE and 2-D PAGE.  
        关键词：Semen Seu Fructus Physalis;protein extraction;SDS-PAGE   
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        发布时间：2024-09-23
      • LIU Wei~,GUO Lei,FAN Ting,DONG Cheng-ming,CHEN Zhi-hong
        Vol. 16, Issue 1, Pages: 18-21(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.010
        摘要：Objective:Determine the content of Saikosaponin a and d by HPLC-ELSD.To analyze different methods of fertilization of the programme as optimum Fertilization one of the targets.Method:The HPLC method was used,chromatography condition was:Agilent TC-C18（4.6 mm×250 mm,5 μm）column, acetonitrile-water（50∶50） as mobile phase,detection is ELSD,drift temperature 93 ℃,air flow 2.5 L·min-1,column temperature 20 ℃.Results:Establishing the fertilizer programme of Radix Bupleuri.Conclusion:The method is accuate,simple,and can provide the basis for optimization method of fertilization scheme by changes of Saikosaponin a and d.  
        关键词：Radix Bupleuri;different fertilizers;saikosaponin A;saikosaponin D   
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        发布时间：2024-09-23
      • LIU Wei,GUO Lei,FAN Ting,DONG Cheng-ming,CHEN Zhi-hong
        Vol. 16, Issue 1, Pages: 18-21(2010)
        摘要：Objective: Determine the content of Saikosaponin a and d by HPLC-ELSD.To analyze different methods of fertilization of the programme as optimum Fertilization one of the targets. Method: The HPLC method was used,chromatography condition was:Agilent TC-C18(4.6 mm×250 mm,5 μm)column, acetonitrile-water (50∶50) as mobile phase,detection is ELSD,drift temperature 93 ℃,air flow 2.5 L·min-1,column temperature 20 ℃. Results :Establishing the fertilizer programme of Radix Bupleuri. Conclusion :The method is accuate, simple,and can provide the basis for optimization method of fertilization scheme by changes of Saikosaponin a and d.  
        关键词：Radix Bupleuri;different fertilizers;saikosaponin A;saikosaponin D   
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        发布时间：2024-09-23
      • Vol. 16, Issue 1, Pages: 21-22(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.011
        摘要：目的:建立喘宁巴布剂中芥子碱硫氰酸盐的高效液相色谱测定方法。方法:采用Kromasil苯基色谱柱（150 mm×4.6 mm,5μm）;以3%醋酸（A）-乙腈（B）为流动相,梯度洗脱;检测波长为326 nm。结果:芥子碱硫氰酸盐在0.040.40μg范围内呈良好线性关系,回归方程Y=3 931.3X-5.995 9,r=0.999 9,平均回收率为99.14%,RSD=1.69%（n=6）。结论:建立的方法用于测定喘宁巴布剂中芥子碱硫氰酸盐的含量简便、可靠、准确,可用于该制剂的质量控制。  
        关键词：喘宁巴布剂;高效液相色谱法;芥子碱硫氰酸盐   
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      • Vol. 16, Issue 1, Pages: 23-25(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.012
        摘要：目的:测定地黄、不同提取物及熟地黄中是否含有梓醇。有效地去除地黄水提取物所含的梓醇,为进一步提取、纯化地黄中的地黄寡糖、梓醇,比较、验证地黄寡糖和梓醇的降糖作用,奠定一定的基础。方法:采用甲醇为溶媒,超声提取梓醇,流动相用甲醇-0.1%磷酸溶液（1∶99）,流速1.0 mL.min-1,C18色谱柱分离,检测波长210 nm,高效液相色谱法测定。结果:地黄与熟地黄中梓醇含量存在显著性差异,地黄水提取物含有少量的梓醇,15%乙醇洗脱物中不含梓醇,地黄水提取物甲醇超声提取后的残渣不含梓醇。结论:该方法分离效果好、准确、快速、干扰少,适用于检测地黄、熟地黄及地黄不同提取物中是否含有梓醇。  
        关键词：地黄;熟地黄;地黄不同提取物;高效液相色谱   
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      • Vol. 16, Issue 1, Pages: 25-27(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.013
        摘要：目的:建立治带片的质量控制方法。方法:采用薄层色谱法对治带片中的苍术、金樱子进行薄层鉴别,采用高效液相色谱法对治带片中苦参碱和氧化苦参碱进行含量测定。结果:薄层色谱中,色谱斑点清晰,阴性无干扰;测得的苦参碱和氧化苦参碱质量分别在0.333.3μg（r=0.999 8）和0.0380.38μg（r=0.999 9）时与色谱峰面积之间线性关系良好,二者平均回收率分别为100.4%、101.5%,RSD分别为1.4%、1.5%。结论:该方法简便、快速、准确,可作为治带片质量控制的一个有效方法。  
        关键词：治带片;质量标准;薄层鉴别;高效液相色谱法   
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      • Vol. 16, Issue 1, Pages: 28-29(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.014
        摘要：目的:测定白银宫血灵胶囊中东莨菪内酯的含量,建立该制剂的质量控制方法。方法:采用高效液相色谱（HPLC）法,使用Kromasil C18柱（4.6 mm×250 mm,5μm）;以甲醇-0.5%冰醋酸（40∶60）为流动相;检测波长为345 nm。结果:东莨菪内酯在0.03860.7728μg范围内线性关系良好,相关系数为r=0.9999,平均回收率为98.95%,RSD=1.1%。结论:本法简便、快速、重复性好,可作为本品的质量控制方法。  
        关键词：白银宫血灵胶囊;高效液相色谱法;东莨菪内酯   
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      • Vol. 16, Issue 1, Pages: 30-31(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.015
        摘要：目的:建立高效液相法测定乌梅丸中柠檬酸含量的方法。方法:供试品采用甲醇超声提取。分析采用Agilent SBC18色谱柱,流动相:A甲醇-B 0.8%NH4H2PO4水溶液（H3PO4调pH值2.8）（5∶95）,流速:0.5 mL.min-1,柱温:25℃,检测波长:214 nm。结果:样品平均回收率为101%（RSD=1.8%）,样品平均含量为9.74%。结论:本法准确、灵敏、重复性好,为乌梅丸复方的质量控制提供了依据。  
        关键词：乌梅丸;柠檬酸;含量测定;高效液相色谱法   
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      • WEN You-min,YANG Xiao-ying~,MA Xiao-yan
        Vol. 16, Issue 1, Pages: 32-34(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.045
        摘要：Objective:To investigate the stability of Tanreqing injection and its compatibility with Aminoglycosides.Methods: The insoluble particles of Tanreqing injection in five kinds of infusion solution were detected respectively by light-blockage method.The contents of Baicalin in two mixed solutions within 8h were determined by HPLC respectively.And the appearance and pH value of the mixtures were compared with those before mixing.The changes after Tanreqing injection mixed with isepamicin,etimicin or gentamycin were observed.Results: After Tanreqing injection was mixed with each one of the five kinds of infusion solution,the number of particles increased.And the contents of Baicalin and pH value had no obvious change in the solution mixed with 5% GS or 0.9% NS.There are visible substances in Tanreqing injection mixed with aminoglycosides.Conclusions: The insoluble particles of Tanreqing injection should be monitored in clinical application.There is incompatibility between Tanreqing injection and aminoglycosides.  
        关键词：Tanreqing injection;insoluble particles;aminoglycosides;compatibility stability   
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      • WEN You-min,YANG Xiao-ying,MA Xiao-yan
        Vol. 16, Issue 1, Pages: 32-34(2010)
        摘要：Objective: To investigate the stability of Tanreqing injection and its compatibility with Aminoglycosides. Methods: The insoluble particles of Tanreqing injection in five kinds of infusion solution were detected respectively by light\|blockage method. The contents of Baicalin in two mixed solutions within 8h were determined by HPLC respectively. And the appearance and pH value of the mixtures were compared with those before mixing. The changes after Tanreqing injection mixed with isepamicin, etimicin or gentamycin were observed. Results: After Tanreqing injection was mixed with each one of the five kinds of infusion solution, the number of particles increased. And the contents of Baicalin and pH value had no obvious change in the solution mixed with 5% GS or 0.9% NS. There are visible substances in Tanreqing injection mixed with aminoglycosides. Conclusions: The insoluble particles of Tanreqing injection should be monitored in clinical application. There is incompatibility between Tanreqing injection and aminoglycosides.  
        关键词：Tanreqing injection;insoluble particles;aminoglycosides;compatibility stability   
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      • Vol. 16, Issue 1, Pages: 35-36(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.016
        摘要：目的:建立芪参玉液胶囊中丹酚酸B含量测定方法。方法:采用高效液相色谱法,色谱柱:Hypersil ODS C18（5μm,4.6 mm×250 mm）;流动相:甲醇-乙腈-甲酸-水（30∶10∶1∶59）。检测波长:286 nm;流速:1.0 mL.min-1。结果:丹酚酸B的回收率为98.60%,RSD为1.09%（n=6）。结论:该方法简便、准确、可靠,适用于芪参玉液胶囊中丹酚酸B的含量测定。  
        关键词：丹酚酸B;高效液相色谱法;芪参玉液胶囊;含量测定   
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      • Vol. 16, Issue 1, Pages: 37-39(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.017
        摘要：目的:建立壮阳填精口服液中淫羊霍苷的含量测定方法。方法:采用TLC法对淫羊藿、黄芪、蛇床子进行定性鉴别;采用HPLC法测定本制剂中淫羊藿苷的含量。以乙腈-水（31∶69）为流动相,流速为0.8 mL.min-1,检测波长为270 nm,柱温为室温,进样量为15μL。结果:在选定的薄层色谱条件下,层析斑点清晰,分离效果较好。HPLC法测定淫羊藿苷的线性范围为0.4692.814μg,r=0.9995。平均回收率为100.4%（n=6）,RSD为1.7%。结论:该方法简便可行、专属性强、重复性好,可用于控制该制剂的质量。  
        关键词：壮阳填精口服液;薄层鉴别;含量测定;淫羊藿苷   
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        发布时间：2024-09-23
      • NI Shu-mao,QIAN Da-wei~,SHANG Er-xin,DUAN Jin-ao~,GUO Jian-ming,TANG Yu-ping
        Vol. 16, Issue 1, Pages: 39-45(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.028
        摘要：Objective:To analyze and identify the chemical composition of Dachunxiong Fang by LC-MS.Method:The analysis was performed on an AQCUITY UPLC BEH C18（2.1 mm×100 mm,1.7 μm） column.The mobile phases were acetonitrile and water in gradient elution.ESI source was used and data were acquited in positive and negative mode.Result:The structures of 17 compounds of Dachunxiong Fang were identified by direct comparison with MS and MS2 of Q-TOF,the elemental compositions analysis,the standard substance and the data of the literature.Furthermore,the types of the compounds were phenols,organic acids,phthalides and compounds containg nitrogen.Addition,the mass spectrum characterization of the major chemical composition of Dachunxiong Fang are analyzed.Conclusion:It is a rapid and accurate method that the composition of compound prescription of traditional Chinese medicine can be identified in terms of the separation of ultra high performance liquid chromatography,the accurate molecular weights measured by MS and other information,which can trace to the source of compounds in Dachuanxiong Fang.  
        关键词：Dachuanxiong Fang;Szedwan Lovage Rhizome;Tall Gastrodia Tuber;UPLC-ESI-QTOF   
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      • NI Shu-mao,QIAN Da-wei,SHANG Er-xin,GUO Jian-ming,TANG Yu-ping
        Vol. 16, Issue 1, Pages: 39-45(2010)
        摘要：Objective: To analyze and identify the chemical composition of Dachunxiong Fang by LC-MS. Method :The analysis was performed on an AQCUITY UPLC BEH C18(2.1 mm×100 mm,1.7 μm) column.The mobile phases were acetonitrile and water in gradient elution.ESI source was used and data were acquited in positive and negative mode. Result :The structures of 17 compounds of Dachunxiong Fang were identified by direct comparison with MS and MS2 of Q-TOF, the elemental compositions analysis, the standard substance and the data of the literature. Furthermore, the types of the compounds were phenols, organic acids, phthalides and compounds containg nitrogen. Addition, the mass spectrum characterization of the major chemical composition of Dachunxiong Fang are analyzed. Conclusion :It is a rapid and accurate method that the composition of compound prescription of traditional Chinese medicine can be identified in terms of the separation of ultra high performance liquid chromatography, the accurate molecular weights measured by MS and other information, which can trace to the source of compounds in Dachuanxiong Fang.  
        关键词：Dachuanxiong Fang;Szedwan Lovage Rhizome;Tall Gastrodia Tuber;UPLC-ESI-QTOF   
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      • WANG Ming-wei~1,WANG Xue-li~2,XING Dong-ming~2,LEI Fan~2,DU Li-jun~
        Vol. 16, Issue 1, Pages: 46-50(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.029
        摘要：Objective: To investigate the pharmacokinetics of berberine in Coptidis Rhizoma extract in rat intestinal and bile.Method: A simple and accurate high-performance liquid chromatography method was used to determine the concentration of berberine in the rat intestinal and bile samples.Result: Following the intravenous administration of Coptidis Rhizoma extract,B/P（concentration in the bile/concentration in the plasma） of berberine was determined to be 69.1～497.9 from 0.25 h to 4 h.Bile excretion was one of the main excretion ways for rats to eliminate berberine of Coptidis Rhizoma alkaloids.Most of berberine in the intestinal was from the bile.While bile was discharged from rats,berberine still could be determined in the intestinal.Conclusion: The results proved that berberine could be transported from blood to intestinal in the animal model with a direction from basolateral membrane to apical membrane.P-glycoprotein is involved in the transport mechanism.  
        关键词：berberine;intestinal;bile;transport   
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      • WANG Ming-wei,WANG Xue-li,XING Dong-ming,LEI Fan,DU Li-jun
        Vol. 16, Issue 1, Pages: 46-50(2010)
        摘要：Objective: To investigate the pharmacokinetics of berberine in Coptidis Rhizoma extract in rat intestinal and bile. Method: A simple and accurate high-performance liquid chromatography method was used to determine the concentration of berberine in the rat intestinal and bile samples. Result: Following the intravenous administration of Coptidis Rhizoma extract, B/P (concentration in the bile/concentration in the plasma) of berberine was determined to be 69.1~497.9 from 0.25 h to 4 h. Bile excretion was one of the main excretion ways for rats to eliminate berberine of Coptidis Rhizoma alkaloids. Most of berberine in the intestinal was from the bile. While bile was discharged from rats, berberine still could be determined in the intestinal. Conclusion: The results proved that berberine could be transported from blood to intestinal in the animal model with a direction from basolateral membrane to apical membrane. P-glycoprotein is involved in the transport mechanism.  
        关键词：berberine;intestinal;bile;transport   
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      • Vol. 16, Issue 1, Pages: 51-52(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.030
        摘要：目的:建立降香挥发油中指标成分橙花叔醇在大鼠体内的HPLC检测方法。方法:血浆经甲醇沉淀蛋白,以shim-pack vp C18（150 mm×4.6 mm,5μm）为色谱柱,以水（A相）-甲醇（B相）为流动相,采用梯度洗脱:016 min B相由75%线性递增至92%,1617 min B相由92%线性递减至75%,流速1.0 mL.min-1,柱温30℃;检测波长213 nm。结果:橙花叔醇血药浓度为0.052μL.mL-1时与峰面积之间线性关系良好（r2=0.981 2）,低、中、高3个浓度的日内、日间精密度、样品的稳定性、提取回收率RSD均低于10%。结论:此方法简便、快速、准确可靠,适用于大鼠血浆中降香挥发油指标成分橙花叔醇的浓度测定。  
        关键词：降香挥发油;高效液相色谱法;橙花叔醇   
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      • CUI Han-ming~,BAI Ge,HUANG Shi-jing,ZHANG Chun-guang,ZHANG Qiu-yan
        Vol. 16, Issue 1, Pages: 53-56(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.031
        摘要：Objective:To establish a measurement method for determining the levels of monoamines neurotransmitter and their metabolites in rat plasma by HPLC with Coularray Detector-2.Methods:The levels of NE,E,DA,HVA and 5-HT in rat plasma were determined by Agilent 1200 HPLC with ESA Coularray Detector-2.Mobile phase consisted of 80 mmol·L-1 Citric acid monohydrate,73.4 mmol·L-1 Citric acid trisodium salt,0.12 mmol·L-1 1-Octanesulfonic acid sodium salt,0.1 mmol·L-1 EDTA-Na2 and 18% methanol mixture solution（pH4.3）.Flow rate was 1.0 mL·min-1.The Column was Zorbax Eclipse XDB-C18（4.6 mm×150 mm, 5 μm）and Easyguard guard column at（25 ℃.） The voltage of electrode was-150 mv and 400 mv.Results:The minimum measurement limit of NE,E,DA,HVA and 5-HT were 2.0,3.0,5.4,5.2 and 11.1 ng·mL-1 respectively.The average recoveries were 99.72%,85.33%,83.15%,120.43% and 103.56%,respectively.The RSD of intra-day（n=6） and inter-day（n=3） were less than 9.12%.Conclusion:The method was easy to operate,rapid,accuracy and high in sensitivity,and could be applied in quantitative determination and study of monoamines neurotransmitters and their metabolites in rat plasma.  
        关键词：monoamines neurotransmitter;HPLC;Coularray ECD;catecholamines   
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      • Vol. 16, Issue 1, Pages: 50-56(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.044
          
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      • Vol. 16, Issue 1, Pages: 51-52(2010)
        摘要：Objective:To establish a measurement method for determining the levels of monoamines neurotransmitter and their metabolites in rat plasma by HPLC with Coularray Detector-2．Methods:The levels of NE，E，DA，HVA and 5-HT in rat plasma were determined by Agilent 1200 HPLC with ESA Coularray Detector-2．Mobile phase consisted of 80 mmol·L-1 Citric acid monohydrate，73.4 mmol·L-1 Citric acid trisodium salt，0.12mmol·L-1 1-Octanesulfonic acid sodium salt,0.1 mmol·L-1 EDTA-Na2 and 18% methanol mixture solution(pH4.3)．Flow rate was 1.0 mL·min-1 The Column was Zorbax Eclipse XDB-C18(4.6 mm X 150 mm，5 μm)and Easyguard guard column at 25℃．The voltage of electrode was -150 mV and 400 mv．Results:The minimum measurement limit of NE，E，DA，HVA and 5-HT were 2.0，3.0，5.4，5.2 and 11.1 ng·mL-1 respectively．The average recoveries were 99.72%，85.33%，83.15%，120.43% and 103.56%，respectively．The RSD of intra-day(n=6)and inter-day(n=3)were less than 9.12%．Conclusion:The method was easy to operate，rapid，accuracy and high in sensitivity，and could be applied in quantitative determination and study of monoamines neurotransmitters and their metabolites in rat plasma．  
          
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      • CUI Han-ming,BAI Ge,HUANG Shi-jing,ZHANG Chun-guang,ZHANG Qiu-yan
        Vol. 16, Issue 1, Pages: 53-56(2010)
        摘要：Objective :To establish a measurement method for determining the levels of monoamines neurotransmitter and their metabolites in rat plasma by HPLC with Coularray Detector-2. Methods :The levels of NE, E, DA, HVA and 5-HT in rat plasma were determined by Agilent 1200 HPLC with ESA Coularray Detector-2. Mobile phase consisted of 80 mmol·L-1 Citric acid monohydrate, 73.4 mmol·L-1 Citric acid trisodium salt, 0.12 mmol·L-1 1-Octanesulfonic acid sodium salt, 0.1 mmol·L-1 EDTA-Na2 and 18% methanol mixture solution(pH4.3). Flow rate was 1.0 mL·min-1.The Column was Zorbax Eclipse XDB-C18 (4.6 mm×150 mm, 5 μm)and Easyguard guard column at 25 ℃. The voltage of electrode was -150 mv and 400 mv. Results :The minimum measurement limit of NE, E, DA, HVA and 5-HT were 2.0, 3.0, 5.4, 5.2 and 11.1 ng·mL-1 respectively. The average recoveries were 99.72％, 85.33％, 83.15％, 120.43％ and 103.56％, respectively. The RSD of intra-day (n=6) and inter-day (n=3) were less than 9.12%. Conclusion :The method was easy to operate, rapid, accuracy and high in sensitivity, and could be applied in quantitative determination and study of monoamines neurotransmitters and their metabolites in rat plasma.  
        关键词：monoamines neurotransmitter;HPLC;Coularray ECD;catecholamines   
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      • Vol. 16, Issue 1, Pages: 130-131(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.002
          
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      • DU Xin-liang,SUI Feng,ZHANG Chang-bin,ZHAO Bao-sheng,LIU Hong-bin,YAN Mei-juan,LI Lan-fang,GUO Shu-ying,Huo Hai-ru,JIANG Ting-liang
        Vol. 16, Issue 1, Pages: 57-61(2010)
        摘要：Objective: To investigate the influence of Sangjuyin drug serum on the expression of TLRs on RAW264.7. Methods: RAW264.7 cell line was stimulated with the drug serum containing Sangjuyin. Expressions of TLRs mRNA were detected with real-time PCR after 24 hours. Results: The serum contained Sangjuyin could upregulated the expression of TLR-3 mRNA and TLR-7 mRNA in RAW264.7 cells. Conclusion: Promoting the expression of Toll-like receptors mRNA may be the elementary basis of Sangjuyin to play the effect.  
        关键词：Sangjuyin;Drug Serum;pulmonary macrophage;TLR-7;TLR-4   
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      • Vol. 16, Issue 1, Pages: 131-132(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.043
          
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      • DU Xin-liang,SUI Feng,ZHANG Chang-bin,ZHAO Bao-sheng,LIU Hong-bin,YAN Mei-juan,LI Lan-fang,GUO Shu-ying,HUO Hai-ru~,JIANG Ting-liang
        Vol. 16, Issue 1, Pages: 57-61(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.032
        摘要：Objective: To investigate the influence of Sangjuyin drug serum on the expression of TLRs on RAW264.7.Methods: RAW264.7 cell line was stimulated with the drug serum containing Sangjuyin.Expressions of TLRs mRNA were detected with real-time PCR after 24 hours.Results: The serum contained Sangjuyin could upregulated the expression of TLR-3 mRNA and TLR-7 mRNA in RAW264.7 cells.Conclusion: Promoting the expression of Toll-like receptors mRNA may be the elementary basis of Sangjuyin to play the effect.  
        关键词：Sangjuyin;Drug Serum;pulmonary macrophage;TLR-7;TLR-4   
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      • CHENG Bin-bin,LV Xiang,LI Bai,YUE Xiao-qiang,LING Chang-quan
        Vol. 16, Issue 1, Pages: 61-64(2010)
        摘要：Objective: To investigate the difference in effect of components formula of Shengmai Powder and that of decoction of Shengmai Powder (SMP) on regulating glucocorticoid receptor (GR) in rats after thermal injury. Methods: A total of 32 male SD rats were randomly assigned into normal control group, thermal injury group, components of SMP group and decoction of SMP group, with 8 rats in each group. Rats in normal control and thermal injury groups were intragastrically administered with normal saline (NS) at room temperature once daily and rats in components and decoction of SMP groups were treated with mixture of aqueous extracts of Ophiopogonis Japonic, Fructus schizandrae Chinensis and ginsenosides or decoction of SMP respectively once daily for a week. After the last administration, rats except those in normal control underwent thermal injury for one hour, and then were sacrificed immediately by decapitation. Blood serum was collected, and serum corticosterone (CS) and adrenocorticotropic hormone (ACTH) levels were determined with enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) respectively. Cytosols from liver, lung and kidney were used to determine the GR binding capacity by radioligand receptor binding assay. The results were analyzed by one point analysis. Results: The simple point analysis showed that the binding capacities of GR in liver, lung and kidney cytosols after thermal injury were obviously lower than that of normal control (P＜0.01). GR binding capacities in liver and lung cytosols of components and decoction of SMP groups were higher than that of thermal injury group. GR binding capacities in liver cytosol of components of SMP group was higher than that of decoction of SMP group, but no significant differences were detected in lung and kidney cytosols between the two groups. Both the Serum CS and ACTH levels of thermal injury group, components of SMP group and decoction of SMP group were higher than that of normal control group. But there was no significant difference between the three groups. Conclusion: The effect of component of SMP on up-regulating GR is better at least partly than that of decoction of SMP in rats after thermal injury.  
        关键词：Shengmai powder;components formula;thermal injury;glucocorticoid receptor;rats   
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      • CHENG Bin-bin~1,LV Xiang~1,LI Bai~1,YUE Xiao-qiang~2,LING Chang-quan~
        Vol. 16, Issue 1, Pages: 61-64(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.033
        摘要：Objective: To investigate the difference in effect of components formula of Shengmai Powder and that of decoction of Shengmai Powder（SMP） on regulating glucocorticoid receptor（GR） in rats after thermal injury.Methods: A total of 32 male SD rats were randomly assigned into normal control group,thermal injury group,components of SMP group and decoction of SMP group,with 8 rats in each group.Rats in normal control and thermal injury groups were intragastrically administered with normal saline（NS） at room temperature once daily and rats in components and decoction of SMP groups were treated with mixture of aqueous extracts of Ophiopogonis Japonic,Fructus schizandrae Chinensis and ginsenosides or decoction of SMP respectively once daily for a week.After the last administration,rats except those in normal control underwent thermal injury for one hour,and then were sacrificed immediately by decapitation.Blood serum was collected,and serum corticosterone（CS） and adrenocorticotropic hormone（ACTH） levels were determined with enzyme-linked immunosorbent assay（ELISA） and radioimmunoassay（RIA） respectively.Cytosols from liver,lung and kidney were used to determine the GR binding capacity by radioligand receptor binding assay.The results were analyzed by one point analysis.Results: The simple point analysis showed that the binding capacities of GR in liver,lung and kidney cytosols after thermal injury were obviously lower than that of normal control（P<0.01）.GR binding capacities in liver and lung cytosols of components and decoction of SMP groups were higher than that of thermal injury group.GR binding capacities in liver cytosol of components of SMP group was higher than that of decoction of SMP group,but no significant differences were detected in lung and kidney cytosols between the two groups.Both the Serum CS and ACTH levels of thermal injury group,components of SMP group and decoction of SMP group were higher than that of normal control group.But there was no significant difference between the three groups.Conclusion: The effect of component of SMP on up-regulating GR is better at least partly than that of decoction of SMP in rats after thermal injury.  
        关键词：Shengmai powder;components formula;thermal injury;glucocorticoid receptor;rats   
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      • WANG Da-ping,WANG Min,WANG Wen-fang,HAN Fu-sen,MA Chang|yu,HE Rong-rong,LI Yuan-bo
        Vol. 16, Issue 1, Pages: 65-67(2010)
        摘要：Objective: To observe the protective effects of Baoligan capsule on acute alcohol induced liver injury in mice. Methods: After oral administration of Baoligan (300 and 600 mg·kg-1) for seven days, the mice were administrated alcohol(8 g·kg-1)to induce liver injury. Hepatic function was evaluated by assessing alanine aminotransferase (ALT) level in plasma. TBARS method was performed to detect the contents of malondialdehyde (MDA) in liver. Antioxidant capacity of liver was measured by oxygen radical absorbance capacity (ORAC) assay. The contents of glutathione (GSH) were detected in liver by HPLC method. Hepatic glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were detected by enzymology method. Results: Baoligan capsule decreased the levels of ALT in plasma and the content of MDA in liver. It also increased the levels of ORAC, GSH, GSH-Px and SOD in liver as compared with alcoholic control group. Conclusion : Baoligan capsule exerted protective effects on liver injury in mice loaded with alcohol.  
        关键词：baoligan;alcohol;liver injury   
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      • WANG Da-ping~1,WANG Min~2,WANG Wen-fang~1,HAN Fu-sen~1,MA Chang-yu~1,HE Rong-rong~
        Vol. 16, Issue 1, Pages: 65-67(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.034
        摘要：Objective: To observe the protective effects of Baoligan capsule on acute alcohol induced liver injury in mice.Methods: After oral administration of Baoligan(300 and 600 mg·kg-1) for seven days,the mice were administrated alcohol(8 g·kg-1)to induce liver injury.Hepatic function was evaluated by assessing alanine aminotransferase（ALT） level in plasma.TBARS method was performed to detect the contents of malondialdehyde（MDA） in liver.Antioxidant capacity of liver was measured by oxygen radical absorbance capacity（ORAC） assay.The contents of glutathione（GSH） were detected in liver by HPLC method.Hepatic glutathione peroxidase （GSH-Px） and superoxide dismutase（SOD） were detected by enzymology method.Results: Baoligan capsule decreased the levels of ALT in plasma and the content of MDA in liver.It also increased the levels of ORAC,GSH,GSH-Px and SOD in liver as compared with alcoholic control group.Conclusion: Baoligan capsule exerted protective effects on liver injury in mice loaded with alcohol.  
        关键词：baoligan;alcohol;liver injury   
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      • LI Dong,CHEN Zhi-wu,CEN De-yi,WANG Di-sheng
        Vol. 16, Issue 1, Pages: 68-71(2010)
        摘要：Objective: To study the anti-bradycardic mechanism of Ganfu granule in rats. Methods: The bradycardic model in rats was induced by propranolol (Pro). the activity of glutathione peroxidase(GSH-PX),superoxide dismutase (SOD) and the content of Malonydialdehyde (MDA) in heart tissue were detected; the content of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) in heart tissue as well as the content of calcitonin gene-related peptide (CGRP),endothelin (ET) in plasma were detected by radioimmunoassay. Results: Ganfu granule (30,90,270 mg·kg-1) significantly inhibited the Pro-induced decrease of heart rate in rats, reduced the content of MDA,cGMP in heart tissue, markedly increased the content of CGRP in plasma and the ratio of cAMP/cGMP in heart tissue. Gan granule(90, 270 mg·kg-1) markedly increased the activity of SOD and GSH-PX in heart tissue; Compare with model group, the changes of the content of ET in plasma and cAMP in heart tissue were not obviouse. Conclusion: The anti-bradycardic mechanism of Ganfu granule may be related to anti-free radicals, increasing CGRP, regulating the balance between cAMP and cGMP.  
        关键词：bradycardia;free radicals;calcitonin gene-related peptide;cyclic adenosine monophosphate;cyclic guanosine monophosphate \=   
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      • LI Dong~1,CHEN Zhi-wu~2,CEN De-yi~2,WANG Di-sheng~
        Vol. 16, Issue 1, Pages: 68-71(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.035
        摘要：Objective:To study the anti-bradycardic mechanism of Ganfu granule in rats.Methods: The bradycardic model in rats was induced by propranolol（Pro）. the activity of glutathione peroxidase（GSH-PX）,superoxide dismutase（SOD） and the content of Malonydialdehyde（MDA） in heart tissue were detected;the content of cyclic adenosine monophosphate（cAMP）,cyclic guanosine monophosphate（cGMP） in heart tissue as well as the content of calcitonin gene-related peptide（CGRP）,endothelin（ET） in plasma were detected by radioimmunoassay.Results: Ganfu granule(30,90,270 mg·kg-1) significantly inhibited the Pro-induced decrease of heart rate in rats,reduced the content of MDA,cGMP in heart tissue,markedly increased the content of CGRP in plasma and the ratio of cAMP/cGMP in heart tissue. Gan granule(90,270 mg·kg-1) markedly increased the activity of SOD and GSH-PX in heart tissue;Compare with model group,the changes of the content of ET in plasma and cAMP in heart tissue were not obviouse.Conclusion:The anti-bradycardic mechanism of Ganfu granule may be related to anti-free radicals,increasing CGRP,regulating the balance between cAMP and cGMP.  
        关键词：bradycardia;free radicals;calcitonin gene-related peptide;cyclic adenosine monophosphate;cyclic guanosine monophosphate   
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      • Vol. 16, Issue 1, Pages: 71-73(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.036
        摘要：目的:考察玉屏风散乙酸乙酯萃取物（YA）抑制迟发型超敏反应的效应并分析其化学成分。方法:以2,4-二硝基氯苯（DNCB）诱导小鼠变应性接触性皮炎（ACD）,观察不同剂量YA对耳廓肿胀度的影响;以高效液相-质谱联用法（HPLC-MS）对YA的化学成分进行初步分析。结果与结论:YA可剂量依赖性的抑制小鼠变应性接触性皮炎,改善炎症病理状态;HPLC结果表明有21个主要表达的色谱峰,HPLC-MS分析提示其中包括黄芪黄酮（苷）、皂苷、防风色原酮（苷）等成分。YA中的成分与其抑制迟发型超敏反应效应之间的关系有待进一步研究。  
        关键词：玉屏风散;变应性接触性皮炎;迟发型超敏反应;高效液相-质谱联用法   
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      • Vol. 16, Issue 1, Pages: 74-75(2010)
        摘要：Objective:To observe the effects of Chai Shao He Wei Granules (CSHW) on chronic superficial gastritis in mouse model induced by methimazole．Methods:Established mice model of chronic superficial gastritis by administrating methimazole for 28 days；Three weeks after drug administration histopathology of gastric mucosa was observed by HE stain．The contents of PGE2，phosphatide and aminohexose in gastric mucosa were detected by chemical colorimetry；and the content of serum gastrin was detected by radioimmunoassay．Results:Compared with model group，CSHW high dose group could reduce the pathological damage of chronic superficial gastritis in mice，increase the content of aminohexose，elevate the levels of PGE2 and phosphatide and reduce the level of gastrin in serum，respectively．Conclusions:CSHW could obviously reduce the inflammation and damage in gastric mucosa of chronic superficial gastritis induced by methimazole in mice and protect the gastric mucosa．The effect may be related to the increase of aminohexose in gastric mucosa，raise of phosphatide and PGE2，reduction of serum gastrin．  
          
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      • Vol. 16, Issue 1, Pages: 74-75(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.037
        摘要：目的:观察静安口服液对小儿多发性抽动症（Tourette综合征）模型大鼠脑组织、血浆多巴胺（DA）和高香草酸（HVA）含量的影响。方法:Tourette综合征模型用ip DOI（连续20d）的方法,分为空白对照组、模型组、静安口服液组、硫必利组4组。多巴胺（DA）和高香草酸（HVA）用HPLC法测定。结果:静安口服液能降低脑组织中DA和HVA含量（P<0.01）,作用与硫必利相似。血浆中DA含量极微量,血浆中HVA未检出。结论:静安口服液模型组大鼠脑组织DA和HVA含量较空白对照组明显升高,而静安口服液、硫必利二组含量较模型组明显降低,因此,推测静安口服液作用机理与调整脑内DA和HVA神经递质,减少DA和HVA的异常释放,从而降低突触前多巴胺神经元的过度支配有关。  
        关键词：静安口服液;小儿多发性抽动症;多巴胺;高香草酸;大鼠   
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      • WANG Xin,LI Min,XU Wen-pin,LI Wei-dong
        Vol. 16, Issue 1, Pages: 76-79(2010)
        摘要：Objective: To observe the effects of Chai Shao He Wei Granules (CSHW) on chronic superficial gastritis in mouse model induced by methimazole. Methods: Established mice model of chronic superficial gastritis by administrating methimazole for 28 days; Three weeks after drug administration histopathology of gastric mucosa was observed by HE stain. The contents of PGE2, phosphatide and aminohexose in gastric mucosa were detected by chemical colorimetry; and the content of serum gastrin was detected by radioimmunoassay. Results: Compared with model group, CSHW high dose group could reduce the pathological damage of chronic superficial gastritis in mice, increase the content of aminohexose,elevate the levels of PGE2 and phosphatide and reduce the level of gastrin in serum, respectively. Conclusions: CSHW could obviously reduce the inflammation and damage in gastric mucosa of chronic superficial gastritis induced by methimazole in mice and protect the gastric mucosa. The effect may be related to the increase of aminohexose in gastric mucosa, raise of phosphatide and PGE2, reduction of serum gastrin.  
        关键词：Chai Shao He Wei Granules;chronic superficial gastritis;mechanism;mice   
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      • WANG Xin~
        Vol. 16, Issue 1, Pages: 76-79(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.038
        摘要：Objective:To observe the effects of Chai Shao He Wei Granules（CSHW） on chronic superficial gastritis in mouse model induced by methimazole.Methods:Established mice model of chronic superficial gastritis by administrating methimazole for 28 days;Three weeks after drug administration histopathology of gastric mucosa was observed by HE stain.The contents of PGE2,phosphatide and aminohexose in gastric mucosa were detected by chemical colorimetry;and the content of serum gastrin was detected by radioimmunoassay.Results: Compared with model group,CSHW high dose group could reduce the pathological damage of chronic superficial gastritis in mice,increase the content of aminohexose,elevate the levels of PGE2 and phosphatide and reduce the level of gastrin in serum,respectively.Conclusions: CSHW could obviously reduce the inflammation and damage in gastric mucosa of chronic superficial gastritis induced by methimazole in mice and protect the gastric mucosa.The effect may be related to the increase of aminohexose in gastric mucosa,raise of phosphatide and PGE2,reduction of serum gastrin.  
        关键词：Chai Shao He Wei Granules;chronic superficial gastritis;mechanism;mice   
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      • TANG Xi-lan,HUANG Li-xin,ZENG Zhi-jun,ZHANG Qi-yun,SHAN Yi-min,XU Guo-liang
        Vol. 16, Issue 1, Pages: 79-82(2010)
        摘要：Objective: To investigate the effects of the flavonoids of Sarcand glabrain(ZJF-HT) on the expansion of mature megakaryocytes and CFU-Meg in vitro. Methods: Megakaryocytes and megakaryocyte progenitor cells were cultured in liquid and semi-solid colony culture systems respectively. The effects of ZJF-HT and ZJF-HT serum on the expansion of mature megakaryocytes and CFU-Meg were observed. Results: ZJF-HT,up to 125 μg·mL-1,could increase the number of mature megakaryocytes and CFU-Meg compared with model group (P<0.05). ZJF-HT serum could significantly increase the number of mature megakaryocytes over the range of 0.39%~6.25% and also significantly promote the formation of CFU-Meg over the range of 0.20%~6.25%(compared with model group, P<0.01). Conclusions: Both ZJF-HT and ZJF-HT serum can promote the expansion of mature megakaryocytes and CFU-Meg.  
        关键词：flavonoids of Sarcand glabrain;megakaryocytes;expansion   
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      • TANG Xi-lan~1,HUANG Li-xin~2,ZENG Zhi-jun~1,ZHANG Qi-yun~1,SHAN Yi-min~1,XU Guo-liang~
        Vol. 16, Issue 1, Pages: 79-82(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.039
        摘要：Objective:To investigate the effects of the flavonoids of Sarcand glabrain（ZJF-HT） on the expansion of mature megakaryocytes and CFU-Meg in vitro.Methods: Megakaryocytes and megakaryocyte progenitor cells were cultured in liquid and semi-solid colony culture systems respectively.The effects of ZJF-HT and ZJF-HT serum on the expansion of mature megakaryocytes and CFU-Meg were observed.Results:ZJF-HT,up to 125 μg·mL-1,could increase the number of mature megakaryocytes and CFU-Meg compared with model group（P<0.05）.ZJF-HT serum could significantly increase the number of mature megakaryocytes over the range of 0.39%～6.25% and also significantly promote the formation of CFU-Meg over the range of 0.20%～6.25%（compared with model group,P<0.01）.Conclusions:Both ZJF-HT and ZJF-HT serum can promote the expansion of mature megakaryocytes and CFUMeg.  
        关键词：flavonoids of Sarcand glabrain;megakaryocytes;expansion   
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      • Vol. 16, Issue 1, Pages: 83-85(2010)
        摘要：Objective:To observe the Pharmacodynamics of Shanlucha Antihypertensive Tablets on the treatment of hypertension with vertigo and headache．Methods:Through the method of tail suspension test，the open field experiment，the synergetic effect with under threshould sodium pentobarbital and the effect on blood pressure and hyperlipemia in rats，the pharmacological role of Shanlucha Antihypertensive tablets on treatment of hypertension was observed．Results:Autokinesis of the mouse was decreased by Shanlucha Antihypertensive；it had a synergetic effect with under threshold sodium pentobarbital for the sleep of the mice，and it had significant sntihypertensive and hypolipidemic effect． Conclusions:Shanlucha Antihypertensive Tablets have an obvious effects on sedative，hypnogenesis，antihypertensive and hypolipidemic actions．  
        关键词：Shanlucha Antihypertensive Tablets;sedation;hypnogenesis;antihypertension;hypolipidemic action   
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      • Vol. 16, Issue 1, Pages: 83-85(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.040
        摘要：目的:探索温胆汤对精神分裂症模型鼠学习记忆功能的影响。方法:采用MORRIS水迷宫检测法,将50只SD大鼠,随机分为正常组、模型组及温胆汤低、中、高剂量共5组,ig给药,1次/d,21 d后用地卓西平马来酸盐（MK801）造模诱发精神分裂症,3 d后进行空间分辨和空间探索实验,记录各组大鼠每天的逃避潜伏期、穿越平台次数及运动轨迹。结果:与模型组相比,温胆汤各组大鼠的逃避潜伏期明显缩短、跨台次数明显增加。结论:温胆汤能改善精神分裂症模型大鼠的学习记忆功能。  
        关键词：温胆汤;精神分裂症;MORRIS水迷宫;学习记忆;大鼠   
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      • LIU Yuan,WEI Huan-ying,LONG Jie-chao,JIANG Zhen-ou
        Vol. 16, Issue 1, Pages: 86-88(2010)
        摘要：Objective: To observe the Pharmacodynamics of Shanlucha Antihypertensive Tablets on the treatment of hypertension with vertigo and headache. Methods: Through the method of tail suspension test, the open field experiment,the synergetic effect with under threshould sodium pentobarbital and the effect on blood pressure and hyperlipemia in rats, the pharmacological role of Shanlucha Antihypertensive tablets on treatment of hypertension was observed. Results: Autokinesis of the mouse was decreased by Shanlucha Antihypertensive; it had a synergetic effect with under threshold sodium pentobarbital for the sleep of the mice, and it had significant sntihypertensive and hypolipidemic effect. Conclusions: Shanlucha Antihypertensive Tablets have an obvious effects on sedative,hypnogenesis,antihypertensive and hypolipidemic actions.  
        关键词：Shanlucha Antihypertensive Tablets;sedation;hypnogenesis;antihypertension;hypolipidemic action   
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      • LIU Yuan~,WEI Huan-ying,LONG Jie-chao,JIANG Zhen-ou
        Vol. 16, Issue 1, Pages: 86-88(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.005
        摘要：Objective:To observe the Pharmacodynamics of Shanlucha Antihypertensive Tablets on the treatment of hypertension with vertigo and headache.Methods: Through the method of tail suspension test,the open field experiment,the synergetic effect with under threshould sodium pentobarbital and the effect on blood pressure and hyperlipemia in rats,the pharmacological role of Shanlucha Antihypertensive tablets on treatment of hypertension was observed.Results: Autokinesis of the mouse was decreased by Shanlucha Antihypertensive;it had a synergetic effect with under threshold sodium pentobarbital for the sleep of the mice,and it had significant sntihypertensive and hypolipidemic effect.Conclusions: Shanlucha Antihypertensive Tablets have an obvious effects on sedative,hypnogenesis,antihypertensive and hypolipidemic actions.  
        关键词：Shanlucha Antihypertensive Tablets;sedation;hypnogenesis;antihypertension;hypolipidemic action   
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      • Vol. 16, Issue 1, Pages: 88-90(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.041
        摘要：目的:观察糖尿病肾病（DN）模型大鼠肾小管上皮细胞标志蛋白CK-18的表达,探讨通络益肾汤对DN模型大鼠肾脏保护作用的机理。方法:采用单侧肾脏切除+STZ诱导DN大鼠模型,大鼠随机分为正常对照组、模型组、通络益肾汤组、西药治疗组。观察各组大鼠治疗后证候积分、空腹血糖（FBG）、肾功能（BUN、Cre）、24 h尿蛋白定量（24 h Upr）及肾小管上皮细胞标志蛋白CK-18的表达。结果:与模型组比较,通络益肾汤组大鼠的证候积分、FBG、BUN、Cre、24 h Upr均明显下降（P<0.05或P<0.01）,肾小管上皮细胞CK-18表达明显升高（P<0.05）。在改善证候积分、恢复肾小管上皮细胞CK-18表达方面,通络益肾汤组明显优于西药治疗组（P<0.05）。结论:通络益肾汤对DN大鼠模型具有一定的治疗作用,其机理可能与提高DN模型大鼠肾小管上皮细胞CK-18表达,抗肾纤维化有关。  
        关键词：通络益肾汤;糖尿病肾病;肾小管上皮细胞转分化   
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      • Vol. 16, Issue 1, Pages: 91-92(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.020
        摘要：目的:从尿液浓缩机制方面研究六味地黄汤的滋阴作用机制。方法:36只成年雄性SD大鼠分为正常对照组、模型组和中药组。采用放免法测定血浆血管升压素（AVP）水平、免疫组化法检测肾髓质水通道蛋白2（AQP2）表达。结果:与正常对照组相比,模型组大鼠的体重、尿量明显减少,AVP水平上升,AQP2表达增加;中药组上述指标恢复到接近正常组或正常组水平。结论:肾阴虚时机体尿液浓缩机制亢进,六味地黄汤的滋阴作用与其抑制亢进的尿液浓缩机制有关。  
        关键词：肾阴虚;六味地黄汤;血管升压素;水通道蛋白2;尿液浓缩机制   
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      • Vol. 16, Issue 1, Pages: 93-94(2010)
        摘要：Objective:To investigate the effects of Yishen Xiezhuo Huayu Decoction on chronic renal failure (CRF) in model rats and explore its action mechanism．Methods:Rats were randomly divided into the traditional Chinese medicine(TCM) Yishen Xiezhuo Huayu Decoction group，the Western medicine enalapril maleate group，the model group and the sham group．The CRF model was induced by 5/6 subtotal nephrectomy in rats．The blood levels of urea nitrogen(BUN)，creatinine(Cre)，nitric oxide (NO)，endothelin (ET)，clacitonin gene-related peptide (CGRP) and pathological changes of residual kidney were detected．Results:Following treatment，the blood levels of BUN and Ser of both the TCM group and the Western medicine group significantly declined compared with those of the model group (P<0.01)；moreover，the decline in the TCM group was more remarkable than in the western medicine group (P<0.05)．Compared with the sham group，the blood levels of ET and CGRP of the model group significantly rose (P<0.01)．Both the drugs studied could effectively lower the blood level of ET and the decreasing extents were significant in both the TCM group (P<0.O1) and the Western medicine group (P<0.05) when compared with the model group．The blood level of CGRP of the TCM group also declined(P<0.05)．There were no significant differences in the blood level of NO of each group．The most severe pathologic changes in renal tissue were observed in the model group and Yishen Xiezhuo Huayu Decoction could alleviate the changes more effectively than enalapfril maleate．Conclusion:Yishen Xiezhuo Huayu Decoction can protect renal functions of CRF rats more effectively than enalapril maleate．The action mechanism of the TCM might include the down-regulation of the blood ET level，and the alleviation of pathologic changes of renal tissues．  
          
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      • Vol. 16, Issue 1, Pages: 93-94(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.021
        摘要：目的:观察息风止痛口服液对小鼠出凝血时间及耐缺氧时间的影响。方法:60只昆明小鼠按体重随机分空白组,阳性药组,息风止痛口服液大、中、小剂量组,采用常压耐缺氧法和脑循环障碍耐缺氧法观察息风止痛口服液对小鼠缺氧生存时间的影响;采用断尾法、玻片法观察息风止痛口服液对小鼠出、凝血时间的影响。结果:与空白组比较,息风止痛口服液大、中剂量给药组缺氧生存时间均较空白组显著延长;息风止痛口服液3个剂量组小鼠出、凝血时间均较空白组显著延长。结论:息风止痛口服液具有延长小鼠耐缺氧时间和延长小鼠出、凝血时间的作用。  
        关键词：偏头痛;息风止痛口服液;小鼠;出凝血时间;耐缺氧时间   
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      • HUANG Fang,ZHANG Xiao-lu,TAO Ming-long
        Vol. 16, Issue 1, Pages: 95-98(2010)
        摘要：Objective: To investigate the effects of Yishen Xiezhuo Huayu Decoction on chronic renal failure (CRF) in model rats and explore its action mechanism. Methods: Rats were randomly divided into the traditional Chinese medicine(TCM) Yishen Xiezhuo Huayu Decoction group, the Western medicine enalapril maleate group, the model group and the sham group. The CRF model was induced by 5/6 subtotal nephrectomy in rats. The blood levels of urea nitrogen(BUN), creatinine(Cre), nitric oxide (NO), endothelin (ET),clacitonin gene-related peptide (CGRP) and pathological changes of residual kidney were detected. Results: Following treatment, the blood levels of BUN and Ser of both the TCM group and the Western medicine group significantly declined compared with those of the model group (P<0.01); moreover, the decline in the TCM group was more remarkable than in the western medicine group (P<0.05). Compared with the sham group, the blood levels of ET and CGRP of the model group significantly rose (P<0.01). Both the drugs studied could effectively lower the blood level of ET and the decreasing extents were significant in both the TCM group (P<0.01) and the Western medicine group (P<0.05) when compared with the model group. The blood level of CGRP of the TCM group also declined (P<0.05). There were no significant differences in the blood level of NO of each group. The most severe pathologic changes in renal tissue were observed in the model group and Yishen Xiezhuo Huayu Decoction could alleviate the changes more effectively than enalapril maleate. Conclusion: Yishen Xiezhuo Huayu Decoction can protect renal functions of CRF rats more effectively than enalapril maleate. The action mechanism of the TCM might include the down-regulation of the blood ET level, and the alleviation of pathologic changes of renal tissues.  
        关键词：Yishen Xiezhuo Huayu Decoction;chronic renal failure;experimental study   
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      • HUANG Fang~,ZHANG Xiao-lu,TAO Ming-long
        Vol. 16, Issue 1, Pages: 95-98(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.022
        摘要：Objective:To investigate the effects of Yishen Xiezhuo Huayu Decoction on chronic renal failure（CRF） in model rats and explore its action mechanism.Methods: Rats were randomly divided into the traditional Chinese medicine（TCM） Yishen Xiezhuo Huayu Decoction group,the Western medicine enalapril maleate group,the model group and the sham group.The CRF model was induced by 5/6 subtotal nephrectomy in rats.The blood levels of urea nitrogen（BUN）,creatinine（Cre）,nitric oxide（NO）,endothelin（ET）,clacitonin gene-related peptide（CGRP） and pathological changes of residual kidney were detected.Results: Following treatment,the blood levels of BUN and Ser of both the TCM group and the Western medicine group significantly declined compared with those of the model group（P<0.01）;moreover,the decline in the TCM group was more remarkable than in the western medicine group（P<0.05）.Compared with the sham group,the blood levels of ET and CGRP of the model group significantly rose（P<0.01）.Both the drugs studied could effectively lower the blood level of ET and the decreasing extents were significant in both the TCM group（P<0.01） and the Western medicine group（P<0.05） when compared with the model group.The blood level of CGRP of the TCM group also declined（P<0.05）.There were no significant differences in the blood level of NO of each group.The most severe pathologic changes in renal tissue were observed in the model group and Yishen Xiezhuo Huayu Decoction could alleviate the changes more effectively than enalapril maleate.Conclusion: Yishen Xiezhuo Huayu Decoction can protect renal functions of CRF rats more effectively than enalapril maleate.The action mechanism of the TCM might include the down-regulation of the blood ET level,and the alleviation of pathologic changes of renal tissues.  
        关键词：Yishen Xiezhuo Huayu Decoction;chronic renal failure;experimental study   
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      • DONG Liu-yi,WEI Qian
        Vol. 16, Issue 1, Pages: 98-101(2010)
        摘要：Objective: To observe the protective effect of Xingnaojing Emulsion (XNJE) on hemorrhagic shock and septic shock in rabbits. Methods: A rabbit model of hemorrhagic shock was established by bloodletting from femoral artery of rabbits; LPS of E.coli was injected to rabbits via vein to induce septic shock. The successful model rabbits were equally and randomly assigned into 5 groups: normal saline (NS) control group, low, middle and high dose of XNJE (25.7,51.4,102.75 mg·kg-1)group and positive control group (treated with Xingnaojing injection, 51.4 mg·kg-1) (n=8 for each group). The antishock effect of XNJE was then observed. Results: In the hemorrhagic shock model, XNJE could obviously heighten the blood pressure, quicken the heart rate, inhibit the abnormal increase of MDA and enhance the activity of SOD in serum.Besides, in the septic shock model, XNJE could lower the death rate of rabbits, extend the survival time, heighten the blood pressure, inhibit the abnormal increase of MDA, lower the content of NO and enhance the activity of SOD in serum. Conclusion: XNJE exerts a protective effect on hemorrhagic shock and septic shock in rabbits, which could be related with the improvement of microcirculation and antioxidation.  
        关键词：Xingnaojing Emulsion;hemorrhagic shock;septic shock;endotoxin;mean arterial pressure   
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      • DONG Liu-yi~,WEI Qian
        Vol. 16, Issue 1, Pages: 98-101(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.023
        摘要：Objective:To observe the protective effect of Xingnaojing Emulsion（XNJE） on hemorrhagic shock and septic shock in rabbits.Methods: A rabbit model of hemorrhagic shock was established by bloodletting from femoral artery of rabbits;LPS of E.coli was injected to rabbits via vein to induce septic shock.The successful model rabbits were equally and randomly assigned into 5 groups: normal saline（NS） control group,low,middle and high dose of XNJE(25.7,51.4,102.75 mg·kg-1)group and positive control group(treated with Xingnaojing injection,51.4 mg·kg-1)（n=8 for each group）.The antishock effect of XNJE was then observed.Results: In the hemorrhagic shock model,XNJE could obviously heighten the blood pressure,quicken the heart rate,inhibit the abnormal increase of MDA and enhance the activity of SOD in serum.Besides,in the septic shock model,XNJE could lower the death rate of rabbits,extend the survival time,heighten the blood pressure,inhibit the abnormal increase of MDA,lower the content of NO and enhance the activity of SOD in serum.Conclusion: XNJE exerts a protective effect on hemorrhagic shock and septic shock in rabbits,which could be related with the improvement of microcirculation and antioxidation.  
        关键词：Xingnaojing Emulsion;hemorrhagic shock;septic shock;endotoxin;mean arterial pressure   
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      • Vol. 16, Issue 1, Pages: 102-104(2010)
        摘要：Objective:To study the effect of different solvent extracts of Blumea riparia (BI) DC on bleeding time (BT)，coagulation time (CT) and plasma recalcification time (RT)．Methods:BT was determined by the cutting tail method in mice．CT was tested by the capillary method and the slice method in mice respectively，and RT was tested in vitro in rabbits．Results:BT，CT and RT were shortened by the different solvent extracts of Blnmea riparia (BI) DC．in some degree；The results also showed that 70％ alcohol extract could significantly shorten BT (P<0.01)，CT (P<0.01) in mice，and had very remarkable shortening RT (P<0.01) effect in rabbit．Conclusion:70％ alcohol extract of Blumea riparia (B1.) DC．has stronger hemostasis effect than other extracts．  
        关键词：Blumea riparia (B1.) DC.;extract;cruor effects;comparison   
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      • Vol. 16, Issue 1, Pages: 102-104(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.024
        摘要：目的:探讨五味子冻干粉改善果蝇睡眠作用的量效与时效关系。方法:在恒温、恒湿、自动光控条件下,以7日龄果蝇作为观察对象,分别给以含药浓度为0.25%,0.5%,1%,2%,4%,8%的培养基（每只培养指管分别含冻干粉30,60,120,240,480,960 mg）,利用果蝇活动监测仪（DAMS）,以静止时间持续5 min以上作为睡眠标准,观察并分析24 h内睡眠总时间的变化,探讨量效关系;在此基础上以不同时长给药,观察其24 h内睡眠总时间变化,探讨时效关系。结果:雌果蝇在8%药物培养基、给药6 d情况下睡眠延长最明显;而雄果蝇在8%药物培养基、给药4 d情况下睡眠延长最明显。结论:五味子冻干粉可以改善果蝇睡眠,并在一定范围内呈现量效和时效关系。  
        关键词：五味子;果蝇;睡眠;量效与时效   
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      • JIANG Jian-ping,CHEN Chen,LAN Ren-qing,LIU Xi-hua
        Vol. 16, Issue 1, Pages: 104-106(2010)
        摘要：Objective: To study the effect of different solvent extracts of Blumea riparia (Bl) DC on bleeding time (BT), coagulation time (CT) and plasma recalcification time (RT). Methods: BT was determined by the cutting tail method in mice. CT was tested by the capillary method and the slice method in mice respectively, and RT was tested in vitro in rabbits. Results: BT,CT and RT were shortened by the different solvent extracts of Blnmea riparia (BI) DC. in some degree; The results also showed that 70% alcohol extract could significantly shorten BT (P<0.01),CT (P<0.01) in mice, and had very remarkable shortening RT (P<0.01) effect in rabbit. Conclusion: 70% alcohol extract of Blumea riparia (Bl.) DC. has stronger hemostasis effect than other extracts.  
        关键词：Blumea riparia (Bl.) DC.;extract;cruor effects;comparison   
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      • JIANG Jian-ping~,CHEN Chen,LAN Ren-qing,LIU Xi-hua
        Vol. 16, Issue 1, Pages: 104-106(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.025
        摘要：Objective: To study the effect of different solvent extracts of Blumea riparia（Bl） DC on bleeding time（BT）,coagulation time（CT） and plasma recalcification time（RT）.Methods: BT was determined by the cutting tail method in mice.CT was tested by the capillary method and the slice method in mice respectively,and RT was tested in vitro in rabbits.Results: BT,CT and RT were shortened by the different solvent extracts of Blnmea riparia（BI） DC.in some degree;The results also showed that 70% alcohol extract could significantly shorten BT（P<0.01）,CT（P<0.01） in mice,and had very remarkable shortening RT（P<0.01） effect in rabbit.Conclusion: 70% alcohol extract of Blumea riparia（Bl.） DC.has stronger hemostasis effect than other extracts.  
          
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      • Vol. 16, Issue 1, Pages: 107-108(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.026
        摘要：目的:观察安痔消炎片止血、抗肛门溃疡的作用。方法:分别采用剪尾法;毛细玻璃管;玻片法;乙醚致大鼠肛门溃疡法试验。结果:安痔消炎片可使小鼠的出血时间和凝血时间明显缩短;减少大鼠肛门溃疡愈合时间。结论:安痔消炎片有较好的凝血、止血、抗肛门溃疡的作用。  
        关键词：安痔消炎片;凝血、止血;抗肛门溃疡   
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      • Vol. 16, Issue 1, Pages: 109-112(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.027
        摘要：目的:观察伊血安颗粒止血、行气止痛等功效及对有效性作出评价。方法:以小鼠出血及凝血时间、兔凝血酶原时间和优球蛋白溶解时间、大鼠血小板聚集性、豚鼠在体子宫收缩性为指标,考察伊血安颗粒的促凝血作用;以及对兔耳皮下血斑吸收的影响,考察其活血作用;以缩宫素诱发小鼠痛经及醋酸致痛为指标,观察其镇痛作用;以对幼龄小鼠子宫重量的影响为指标,考察雌激素样作用。结果:伊血安颗粒有缩短小鼠出血时间和凝血时间、缩短兔凝血酶原时间、延长兔优球蛋白溶解时间的作用,有增强大鼠血小板聚集性的作用趋势;对豚鼠在体子宫有明显的兴奋作用,使其张力明显提高、收缩力明显增强、收缩频率明显加快,但在实验剂量下未见引起强直性收缩。对缩宫素诱发小鼠痛经模型及醋酸所致小鼠腹痛均有一定的镇痛作用。对兔耳皮下血斑吸收及幼龄小鼠子宫重量无明显影响。结论:伊血安颗粒有明显的促凝血、缩宫、镇痛作用,未表现出雌激素样作用。  
        关键词：伊血安颗粒;凝血;活血;镇痛;雌激素   
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      • Vol. 16, Issue 1, Pages: 113-115(2010)
        摘要：This article is an attempt to compare six surfactants for injection，such as polysorbate 80，polyoxyethylene castor oil derivatives，poloxamerl88，lecithin，Solutol HS 15，cyclodextrins，from various countries'standards of pharmacopoeias and respectively gives an overview of their chemical compositions，physical appearance，incompatibility of medicines and safety，which provides reference data for new drug research and clinical study of injections．As for the surfactants for injection that are not recorded in the chinese pharmacopoeia (2005)，we suggest that we introduce those foreign surfactants and make corresponding quality standards by referring to forejgn standards．  
          
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      • Vol. 16, Issue 1, Pages: 113-114(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.003
        摘要：目的:观察足底反射按摩对老年女性生理方面的影响。方法:分别选取20例作治疗组和对照组测定治疗前后的血压、心率、心脏负荷、唾液中分泌型免疫蛋白A（SIgA）及皮质醇含量。结果与对照组比较,治疗组可降低血压和心脏负荷升高唾液中SIgA含量（有统计学意义）。结论:足底反射区的按摩可以改善老年女性的血压、心脏负荷和免疫功能。  
        关键词：足底反射按摩;老年女性;血压;心率;分泌型免疫球蛋白A;皮质醇   
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      • YI Hong,GAO Jin,YANG Hua,SUN Hui
        Vol. 16, Issue 1, Pages: 115-119(2010)
        摘要：This article is an attempt to compare six surfactants for injection, such as polysorbate 80,polyoxyethylene castor oil derivatives, poloxamer188, lecithin, Solutol HS 15, cyclodextrins,from various countries' standards of pharmacopoeias and respectively gives an overview of their chemical compositions, physical appearance, incompatibility of medicines and safety, which provides reference data for new drug research and clinical study of injections. As for the surfactants for injection that are not recorded in the chinese pharmacopoeia (2005), we suggest that we introduce those foreign surfactants and make corresponding quality standards by referring to foreign standards.  
        关键词：injections;surfactants;quality specification;safety   
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      • YI Hong~1,GAO Jin~1,YANG Hua~
        Vol. 16, Issue 1, Pages: 115-119(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.018
        摘要：This article is an attempt to compare six surfactants for injection, such as polysorbate 80,polyoxyethylene castor oil derivatives,poloxamer188,lecithin,Solutol HS 15,cyclodextrins,from various countries’ standards of pharmacopoeias and respectively gives an overview of their chemical compositions,physical appearance,incompatibility of medicines and safety,which provides reference data for new drug research and clinical study of injections.As for the surfactants for injection that are not recorded in the chinese pharmacopoeia（2005）,we suggest that we introduce those foreign surfactants and make corresponding quality standards by referring to foreign standards.  
        关键词：injections;surfactants;quality specification;safety   
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      • ZHOU A-gao,LI Yan
        Vol. 16, Issue 1, Pages: 120-123(2010)
        摘要：Objective: To compare the effect of traditional Chinese medicine with western medicine therapy for chronic atrophic gastritis. Methods: Relevant literatures were reviewed via database and assessed via randomized controlled trial (RCT) scale.A1l reviewed literatures were assessed via Jadad quality scale. Data statistical analyses were performed by RevMan4.1 software. Results: Clinical efficient: since test for heterogeneity: P=0.31,it indicates the data have homogeneity,so we use the fixed effect model. OR=3.99,95%(2.98,5.33);Z=9.34,P<0.000 01. It means that Chinese medicine treatment for chronic atrophic gastritis symptom in improving clinical has obvious advantages compared with western medicine. For Pathologic biopsy and endoscopy, the test for heterogeneity: P=0.000 1,it indicates the data have heterogeneity,so we use the random-effect models OR=4.67,95%(3.64,5.99);Z=12.13,P<0.000 01. It means that Chinese medicine treatment for chronic atrophic gastritis symptom in improving laboratory medicine has obvious advantages compared with western medicine. Conclusion: TCM treatment for chronic atrophic gastritis shows curative effect, and there are obvious advantages compared with western medicine.  
        关键词：chronic atrophic gastritis;traditional Chinese medicine;randomized comparative trial;Meta analysis   
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      • ZHOU A-gao~
        Vol. 16, Issue 1, Pages: 120-123(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.004
        摘要：Objective:To compare the effect of traditional Chinese medicine with western medicine therapy for chronic atrophic gastritis.Methods: Relevant literatures were reviewed via database and assessed via randomized controlled trial（RCT） scale.A1l reviewed literatures were assessed via Jadad quality scale. Data statistical analyses were performed by RevMan4.1 software.Results:Clinical efficient: since test for heterogeneity: P=0.31,it indicates the data have homogeneity,so we use the fixed effect model.OR=3.99,95%（2.98,5.33）;Z=9.34,P<（0.000 01.） It means that Chinese medicine treatment for chronic atrophic gastritis symptom in improving clinical has obvious advantages compared with western medicine.For Pathologic biopsy and endoscopy,the test for heterogeneity: P=（0.000 1）,it indicates the data have heterogeneity,so we use the random-effect models OR=4.67,95%（3.64,5.99）;Z=12.13,P<（0.000 01.） It means that Chinese medicine treatment for chronic atrophic gastritis symptom in improving laboratory medicine has obvious advantages compared with western medicine.Conclusion: TCM treatment for chronic atrophic gastritis shows curative effect,and there are obvious advantages compared with western medicine.  
        关键词：chronic atrophic gastritis;traditional Chinese medicine;randomized comparative trial;Meta analysis   
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      • ZHAO Zhen-dong
        Vol. 16, Issue 1, Pages: 122-125(2010)
        摘要：Objective: To observe the effect of curcumin on anti-liver fibrosis and its mechanism. Methods: Model of liver fibrosis was induced by intraperitoneal injection of dimethylnitrosamine. Rats were divided into 5 groups after four weeks, each group of rats were treated with corresponding drug. The serum HA,PCIII and Hyp in liver tissue were determined.The changes of liver and expression of PDGF-BB,PDGFRβ,ERK1 of liver were observed by immunohistochemistry. The level and of PDGFRβ,ERK1 mRNA were observed by RT-PCR. Results: Curcumin could obviously decrease relevant index on liver fibrosis and the level of Hyp. Pathological results indicated curcumin could obviously improve the pathological lesion of rat liver fibrosis. The protein expression of PDGF-BB,PDGFRβ,ERK1 was inhibited,and the mRNA expression of PDGFRβ,ERK1 mRNA was down regulated by curcumin. Conclusion: Curcumin is effective in treatment of liver fibrosis, and its mechanism of anti-liver fibrosis is related to inhibiting the expression of PDGFRβ,ERK1 in liver.  
        关键词：curcumin;experimental liver fibrosis;mechanism of action   
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      • SU Shu-lan,DUAN Jin-ao,LI Wen-lin,TANG Yu-ping,FAN Xin-sheng
        Vol. 16, Issue 1, Pages: 123-129(2010)
        摘要：The "Eighteen Incompatible Medicaments" has been the important contents of incompatibility of Chinese herbs since JIN and YUAN dynasty. With the raising of medical treatment for human health and scientific cognition level, the incompatibility of Chinese herbs is an increasing concern and becomes a burning and crucial scientific question. This article analyzed the chemical components study situation of "Eighteen Incompatible Medicaments" and discussed the scientific nature based the toxic chemical components changes, mechanisms of action, and so on. These data may provide instructions for mechanism research of "Eighteen Incompatible Medicaments", supply some references for the rational application in clinic.  
        关键词：Eighteen Incompatible Medicaments;chemical compositions;toxicity mechanism   
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      • SU Shu-lan,DUAN Jin-ao~,LI Wen-lin,TANG Yu-ping,FAN Xin-sheng
        Vol. 16, Issue 1, Pages: 123-129(2010) DOI: 10.13422/j.cnki.syfjx.2010.01.019
        摘要：The "Eighteen Incompatible Medicaments" has been the important contents of incompatibility of Chinese herbs since JIN and YUAN dynasty.With the raising of medical treatment for human health and scientific cognition level,the incompatibility of Chinese herbs is an increasing concern and becomes a burning and crucial scientific question.This article analyzed the chemical components study situation of "Eighteen Incompatible Medicaments" and discussed the scientific nature based the toxic chemical components changes,mechanisms of action,and so on.These data may provide instructions for mechanism research of "Eighteen Incompatible Medicaments",supply some references for the rational application in clinic.  
        关键词：Eighteen Incompatible Medicaments;chemical compositions;toxicity mechanism   
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      • Vol. 16, Issue 1, Pages: 137(2010)
          
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