最新刊期
      卷 16 ， 期 2 ， 2010
      • Vol. 16, Issue 2, Pages: 1-3(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.042
        摘要：目的:探讨甜橙枳实黄酮类成分的最佳提取工艺。方法:以甜橙枳实中主要黄酮类成分橙皮苷和柚皮芸香苷含量为指标,研究甜橙枳实提取挥发油后药渣采用乙醇提取黄酮类成分的最佳提取工艺。结果:甜橙枳实提取黄酮类成分的最佳工艺条件为:10倍量70%乙醇回流提取2次,每次0.5 h,提取液静置,上层药液浓缩后与沉降物合并。结论:甜橙枳实含有在冷水中溶解度小的有效成分,在制剂工艺提取后,提取液静置出现的沉降物不应轻易作为杂质弃去。  
        关键词：甜橙枳实;黄酮类成分;提取工艺;沉降物   
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      • Vol. 16, Issue 2, Pages: 3-5(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.043
        摘要：目的:筛选二类中兽药畜禽抗应激颗粒中黄柏总生物碱提取工艺。方法:采用L9（34）正交设计法对黄柏药材提取时所用乙醇浓度、乙醇用量、提取时间、提取次数进行考察;采用单因素考察法对黄柏药材提取粒度进行考察。结果:黄柏提取总生物碱最佳提取工艺为:黄柏细丝23 mm,用药材10倍量75%乙醇回流提取3次,每次提取1.5 h。结论:该工艺稳定,质量可控,可用于产业化生产。  
        关键词：黄柏;总生物碱;提取;正交设计   
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      • Vol. 16, Issue 2, Pages: 6-7(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.044
        摘要：目的:用大孔吸附树脂层析技术分离丹参酮ⅡA。方法:通过HPD-100树脂对丹参酮ⅡA的吸附穿透曲线与静态实验所得饱和吸附量来确定上样量,进行乙醇梯度洗脱,用高效液相色谱检测丹参酮ⅡA,初步确定树脂的洗脱条件进而分析出分离的优化方案。结果:丹参酮ⅡA的分离纯化提取工艺为:丹参酮提取液,用HPD-100大孔吸附树脂,80%乙醇洗脱。结论:该工艺可以将丹参酮ⅡA的浓度由提取液中的17%左右富集到42%左右,浓度提高了2.5倍左右。  
        关键词：丹参;丹参酮ⅡA;微波辅助提取;大孔树脂   
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      • Vol. 16, Issue 2, Pages: 8-10(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.005
        摘要：目的:为保证蒲金凝胶剂最佳疗效,对该方剂的水提部分工艺进行研究,以确定最佳提取工艺。方法:采用正交实验法,以蒲公英与金银花合提的水提液的抑菌效果和绿原酸含量作为水提效果的考核指标,考察浸泡时间A、加水倍数B、提取时间C,确定最佳工艺路线。结果:蒲公英与金银花合提的最佳工艺为在室温条件下,金银花和蒲公英浸泡0.5 h,煎煮2次,分别加入15和10倍的水,提取时间分别为1 h和0.5 h。结论:采用该水提工艺,水提液的抑菌效果最好,稳定性好。  
        关键词：蒲金凝胶剂;正交试验法;绿原酸;水提工艺   
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      • Vol. 16, Issue 2, Pages: 11-13(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.006
        摘要：目的:建立黄连解毒凝胶的制备工艺。方法:根据高分子凝胶的物理化学性质,和黄连解毒提取物中3个有效部位的的物理化学性质,同时结合口服凝胶的特点,对高分子凝胶辅料及制备工艺进行探索。结果:研制出了具有胃黏膜良好黏附性能的黄连解毒口服凝胶。结论:该制备工艺可操作性强,制备工艺可行。  
        关键词：黄连解毒;凝胶;制备工艺   
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      • Vol. 16, Issue 2, Pages: 14-16(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.007
        摘要：目的:建立女贞子中红景天苷的HPLC的含量测定方法,测定女贞子不同市售品、不同炮制品及不同果实部位中红景天苷含量,为女贞子的质量控制研究提供参考。方法:回流提取法获得供试品溶液,HPLC法测定其含量;色谱条件为Kromasil-C18,250 mm×4.6 mm,5μm;流动相:甲醇-水（17∶83）;流速:1 mL.min-1;柱温:40℃;检测波长:275 nm。结果:采用此HPLC法检测药材中红景天苷在0.255.02μg范围内r=0.999 9,表明线性关系良好,回归方程为Y=2.90×105X+9.30×103;回收率为99.52%,RSD为2.95%。结论:该方法能够为女贞子的质量控制提供参考数据。  
        关键词：女贞子;质量控制;红景天苷;高效液相色谱法   
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      • Vol. 16, Issue 2, Pages: 17-19(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.008
        摘要：目的:建立双黄连凝胶的质量控制方法。方法:采用薄层色谱法对双黄连凝胶中的黄芩、金银花、连翘进行鉴别;采用HPLC法对黄芩中的黄芩苷进行含量测定。结果:薄层鉴别斑点清晰,分离度好,对应的空白及辅料均未见干扰;HPLC法测定结果显示色谱峰形好,分离度好,杂质无干扰,黄芩苷在0.231.18μg范围内呈良好的线性关系,黄芩苷的平均回收率为100.6%,RSD=1.57%（n=6）。结论:该方法操作简便,准确,重复性好,能有效的控制双黄连凝胶的质量。  
        关键词：双黄连凝胶;薄层色谱鉴别法;高效液相色谱法   
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      • Vol. 16, Issue 2, Pages: 20-22(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.009
        摘要：目的:采用反相高效液相色谱法测定复方姜黄微囊中姜黄素和胡椒碱的含量。方法:以乙腈-3%磷酸水溶液（40∶60）为流动相,Zorbax Eclipse XDB-C8柱（4.6 mm×150 mm,5μm）为固定相,流速为0.7 mL.min-1,检测波长为425 nm,343nm。结果:姜黄素在0.073 440.367 20μg之间、胡椒碱在0.003 390.016 95μg之间呈线性关系,回收率分别为97.84%和97.40%,RSD分别为1.80%和1.03%。结论:该方法简单,灵敏度高,可用于复方姜黄微囊中姜黄素和胡椒碱的含量检测。  
        关键词：复方姜黄微囊;高效液相;姜黄素;胡椒碱   
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      • Vol. 16, Issue 2, Pages: 23-25(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.010
        摘要：目的:建立气相色谱法（GC）测定中药红曲中油酸和亚油酸的含量。方法:采用石英毛细管柱EC-1000（30 m×320μm,0.25μm）;FID检测器;色谱条件:柱温228℃;进样口280℃;检测器FID 300℃。载气高纯N2（纯度大于99.999 9%）,柱前压力16.0 psi;分流比10∶1,空气450 mL.min-1,H245 mL.min-1。结果:油酸和亚油酸的加样回收率分别为100.1%和99.2%,重复性良好。结论:本法测定结果准确、可靠、快速、简便,可对中药红曲药材中油酸和亚油酸的含量进行控制。  
        关键词：红曲;油酸;亚油酸;气相色谱法   
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      • MAO Yan,YANG Wei-jun,MA Yila·maimaitiyiming,HE Jin-hua
        Vol. 16, Issue 2, Pages: 25-27(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.011
        摘要：Objective:To develop a RP-HPLC method for the quantification of adenosine in Weigan Effervescent Tablets.Methods: The analysis was carried out on a Shimadzu C18 column（250 mm×4.6 mm,S-5 μm）and the mobile phase was a mitture of methanol-water（8∶92）with a flow rate of 1.0 mL· min-1.The detection wavelength was 260 nm,and the column temperature was 35 ℃.Results: The linear response ranges of adenosine was 4.3～33.8 μg（r=（0.999 7））,and the average recovery was 101.0%（n=9,RSD=1.4%）.Conclusion: The method is quick,simple and reproducible for the determination of adenosine in Weigan Effervescent Tablets.  
        关键词：Weigan Effervescent Tablets;adenosine;HPLC;Assay   
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      • MAO Yan,YANG Wei-jun,MA Yila·maimaitiyiming,HE Jin-hua
        Vol. 16, Issue 2, Pages: 26-27(2010)
        摘要：Objective:To develop a RP-HPLC method for the quantification of adenosine in Weigan Effervescent Tablets. Methods: The analysis was carried out on a Shimadzu C18 column(250 mm×4.6 mm,S-5 μm)and the mobile phase was a mitture of methanol-water(8∶92)with a flow rate of 1.0 mL· min-1.The detection wavelength was 260 nm, and the column temperature was 35 ℃. Results: The linear response ranges of adenosine was 4.3~33.8 μg(r=0.999 7), and the average recovery was 101.0%(n=9,RSD=1.4%). Conclusion: The method is quick, simple and reproducible for the determination of adenosine in Weigan Effervescent Tablets.  
        关键词：Weigan Effervescent Tablets;adenosine;HPLC;Assay   
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      • WANG Ping~
        Vol. 16, Issue 2, Pages: 28-31(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.012
        摘要：Objective:To study chemical components of Dangguibuxue decoction.Methods:The compounds were separated on a Hypersil-Hypurity C18（150 mm×2.1 mm,5 μm） analytical column.The column temperature was set at 40 ℃.Mobile phase consisted of methanol and water using a gradient elution.The flow rate was set at 0.2 mL·min-1.UV spectra were obtained by scanning from 200 nm to 370 nm.The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650.Results:The peaks were identified by comparing their UV spectra,their molecular weights and their retention behaviors with data reported in the literature.The ten components seem to be tentatively identified.Conclusions: Because of providing UV and molecular mass as well as retention time,LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.  
        关键词：Dangguibuxue decoction;Radix Astragali;Radix Angelicae Sinensis;HPLC-DAD-MS   
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      • WANG Ping,LIANG Yi-zeng
        Vol. 16, Issue 2, Pages: 28-31(2010)
        摘要：Objective: To study chemical components of Dangguibuxue decoction. Methods :The compounds were separated on a Hypersil-Hypurity C18(150 mm×2.1 mm, 5 μm) analytical column. The column temperature was set at 40 ℃. Mobile phase consisted of methanol and water using a gradient elution. The flow rate was set at 0.2 mL·min-1. UV spectra were obtained by scanning from 200 nm to 370 nm. The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650. Results :The peaks were identified by comparing their UV spectra, their molecular weights and their retention behaviors with data reported in the literature. The ten components seem to be tentatively identified. Conclusions: Because of providing UV and molecular mass as well as retention time, LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.  
        关键词：Dangguibuxue decoction;Radix Astragali;Radix Angelicae Sinensis;HPLC-DAD-MS   
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      • Vol. 16, Issue 2, Pages: 32-33(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.013
        摘要：目的:建立野菊花中酚酸类主要活性成分绿原酸的含量测定方法。方法:采用RP-HPLC方法,色谱柱为AgilentXDB-C18（250 mm×4.6 mm,5μm）,流动相选用乙腈-0.4%磷酸水（13∶87）,流速0.8 mL.min-1,检测波长327 nm,柱温为30℃,样品温度20℃。结果:绿原酸在0.0881.76μg范围内线性关系良好;平均回收率分别为98.8%,RSD小于1.0%;不同产地野菊花中绿原酸的含量均大于1‰。结论:本方法方便、准确、可以用于野菊花药材质量的进一步补充和完善。  
        关键词：反相高效液相色谱;野菊花;绿原酸   
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      • Vol. 16, Issue 2, Pages: 32-33(2010)
        摘要：Objective: To study chemical components of Dangguibuxue decoction. Methods :The compounds were separated on a Hypersil-Hypurity C18(150 mm×2.1 mm, 5 μm) analytical column. The column temperature was set at 40 ℃. Mobile phase consisted of methanol and water using a gradient elution. The flow rate was set at 0.2 mL·min-1. UV spectra were obtained by scanning from 200 nm to 370 nm. The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650. Results :The peaks were identified by comparing their UV spectra, their molecular weights and their retention behaviors with data reported in the literature. The ten components seem to be tentatively identified. Conclusions: Because of providing UV and molecular mass as well as retention time, LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.  
          
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      • Vol. 16, Issue 2, Pages: 34-36(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.014
        摘要：目的:建立郁舒颗粒的质量标准。方法:采用薄层色谱法鉴别栀子、柴胡。采用HPLC法测定本制剂中栀子苷的含量。流动相为乙腈-水（13∶87）,流速为1.0 mL.m in-1,检测波长为238 nm,柱温为室温,进样量为10μL。结果:在选定的薄层色谱条件下,层析斑点清晰,分离效果较好。HPLC法测定栀子苷的线性范围为0.774 41.936μg,r=0.999 6。平均回收率为99.52%（n=6）,RSD为1.10%。结论:该方法简便可行、专属性强、重复性好,可控制该制剂的质量。目的:建立郁舒颗粒的质量标准。方法:采用薄层色谱法鉴别栀子、柴胡。采用HPLC法测定本制剂中栀子苷的含量。流动相为乙腈-水（13∶87）,流速为1.0 mL.min-1,检测波长为238 nm,柱温为室温,进样量为10μL。结果:在选定的薄层色谱条件下,层析斑点清晰,分离效果较好。HPLC法测定栀子苷的线性范围为0.774 41.936μg,r=0.999 6。平均回收率为99.52%（n=6）,RSD为1.10%。结论:该方法简便可行、专属性强、重复性好,可控制该制剂的质量。  
        关键词：郁舒颗粒;薄层鉴别;含量测定;栀子苷   
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      • Vol. 16, Issue 2, Pages: 37-38(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.016
        摘要：目的:建立贝参平喘片中盐酸麻黄碱的含量测定方法。方法:采用高效液相色谱法,以A ichromBond柱（AQ-C18,4.6 mm×250 mm,5μm）为分离柱,以乙腈-0.1%磷酸、0.1%三乙胺溶液（3∶97）为流动相,210 nm为检测波长,柱温为室温。结果:盐酸麻黄碱在（0.0250.25）μg范围内,峰面积与其浓度呈良好线性关系（r=0.999 6）,平均回收率为100.5%,RSD为0.74%（n=5）。结论:该方法简便、准确、重复性好,可作为该制剂含量测定方法。  
        关键词：贝参平喘片;HPLC;盐酸麻黄碱   
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      • WANG Fang~1,LI Xue-qing~2,LIU Yu-lin~
        Vol. 16, Issue 2, Pages: 39-41(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.017
        摘要：Objective:To establish the quality standard for Shenhua capsules.Methods:The TLC method was used to identify Szechwan Lovage Rhizome and Danshen Root and the HPLC method was used to determine tanshinone ⅡA in the preparation.Results:TLC spots clear and specific.A good linear relationship between peak areas and concentrations of tanshinoneⅡA was found at the range of 0.03～0.64 μg with a correlation coefficient of r=（0.999 8）.The average recovery of tanshinoneⅡA was 99.57% and RSD was 0.99%（n=6）.Conclusions:The method is reliable,accurate and specific.It can be used for the quality control of Shenhua Capsules.  
        关键词：Shenhua Capsules;Tanshinone ⅡA;TLC;HPLC   
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      • Vol. 16, Issue 2, Pages: 37(2010)
        摘要：Objective: In order to evaluate the safety of Chinese medicine comprehensively, the development of pesticide residues determination in Chinese medicine by gas chromatography (GC) was summarized in this paper. Methods : Through analytic comparison, the developments of sample pretreatments were summarized systematically, including solid-phase extraction (SPE), gel permeation chromatography (GPC), supercritical fluid extraction (SFE), accelerated solvent extraction (ACE), matrix solid phase dispersion (MSPD), solid-phase micro-extration (SPME) and headspace analysis (HS). In addition, the comparison of gas chromatography detectors such as mass spectrometry (MS), electron-capture detector (ECD), flame photometric detector (FPD) and nitrogen–phosphorus detector (NPD) were also discussed. Results: Gas chromatography is a mature technology to accomplish pesticide residues determination in Chinese medicine. Conclusion: Gas chromatography and relevant new techniques are wildly used in safety evaluation of Chinese medicine and its preparations, which will be benefit for the international development and import of Chinese medicine.  
        关键词：Chinese medicine;pesticide residues;gas chromatography;sample preparation   
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      • CAI Qing-yu~1,ZHANG Yi~1,HAO Te~1,TONG Hui-juan~2,KANG Chen~2,LI Man-ling~
        Vol. 16, Issue 2, Pages: 42-44(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.018
        摘要：Objective:To develop a HPLC method for the determination of palmatine and berberine in Shulitong Granule.Methods:A Kromasil-C18（250 mm×4.6 mm,5 μm）column was used for the HPLC determination.The mobile phase consisted of acetonitrile-0.1 mol/L potassium dihydrogen phosphate（28∶72）,and the flow rate was 1 mL/min.The detection wavelength was 345 nm.Results:A good linear relationship was constructed in the range of 0.016～0.080 μg（r=（0.999 8））,for Palmatine hydrochloride and in the range of 0.027～0.135 μg（r=（0.999 9）） for berberine hydrochloride. The average recovery of palmatine hydrochloride was 99.74%（RSD=2.67%,n=5）,and that of berberine hydrochloride was 103.53%,RSD=1.48%（n=5）.Conclusion:The developed method is simple,reproducible,specific and suitable for the quality control of Shulitong Granule.  
        关键词：Shulitong Granule;berberine hydrochloride;palmatine hydrochloride;HPLC   
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      • Vol. 16, Issue 2, Pages: 16(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.001
          
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      • WANG Fang,LI Xue-qing,LIU Yu-lin,LIU Zi-dong
        Vol. 16, Issue 2, Pages: 39-41(2010)
        摘要：Objective: To establish the quality standard for Shenhua capsules. Methods: The TLC method was used to identify Szechwan Lovage Rhizome and Danshen Root and the HPLC method was used to determine tanshinone ⅡA in the preparation. Results: TLC spots clear and specific.A good linear relationship between peak areas and concentrations of tanshinoneⅡA was found at the range of 0.03~0.64 μg with a correlation coefficient of r=0.999 8. The average recovery of tanshinoneⅡA was 99.57% and RSD was 0.99%(n=6). Conclusions: The method is reliable,accurate and specific.It can be used for the quality control of Shenhua Capsules.  
        关键词：Shenhua Capsules;Tanshinone ⅡA;TLC;HPLC   
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      • Vol. 16, Issue 2, Pages: 36-41(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.015
          
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      • CAI Qing-yu,ZHANG Yi,HAO Te,TONG Hui-juan,KANG Chen,LI Man-ling
        Vol. 16, Issue 2, Pages: 42-44(2010)
        摘要：Objective: To develop a HPLC method for the determination of palmatine and berberine in Shulitong Granule. Methods: A Kromasil-C18(250 mm×4.6 mm, 5 μm)column was used for the HPLC determination.The mobile phase consisted of acetonitrile-0.1 mol/L potassium dihydrogen phosphate (28 ∶72),and the flow rate was 1 mL/min.The detection wavelength was 345 nm. Results: A good linear relationship was constructed in the range of 0.016~0.080 μg (r=0.999 8), for Palmatine hydrochloride and in the range of 0.027~0.135 μg (r=0.999 9) for berberine hydrochloride. The average recovery of palmatine hydrochloride was 99.74% (RSD=2.67%, n=5),and that of berberine hydrochloride was 103.53%,RSD=1.48%(n=5). Conclusion: The developed method is simple, reproducible, specific and suitable for the quality control of Shulitong Granule.  
        关键词：Shulitong Granule;berberine hydrochloride;palmatine hydrochloride;HPLC   
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      • Vol. 16, Issue 2, Pages: 44-47(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.028
          
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      • Vol. 16, Issue 2, Pages: 44(2010)
        摘要：Based on the current foundation of Radix Isatidis, possibility of quality evaluation of Radix Isatidis by bioassay is discussed. That is to evaluate quality by controlling the overall effect of Radix Isatidis. A new idea and method are provided for quality control of Radix Isatidis.  
        关键词：bioassay;Radix Isatidis;quality evaluation   
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      • Vol. 16, Issue 2, Pages: 45-47(2010)
        摘要：Objective: To establish the quality standard for Shenhua capsules. Methods: The TLC method was used to identify Szechwan Lovage Rhizome and Danshen Root and the HPLC method was used to determine tanshinone ⅡA in the preparation. Results: TLC spots clear and specific.A good linear relationship between peak areas and concentrations of tanshinoneⅡA was found at the range of 0.03~0.64 μg with a correlation coefficient of r=0.999 8. The average recovery of tanshinoneⅡA was 99.57% and RSD was 0.99%(n=6). Conclusions: The method is reliable,accurate and specific.It can be used for the quality control of Shenhua Capsules.  
          
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      • Vol. 16, Issue 2, Pages: 93-103(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.046
          
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      • Vol. 16, Issue 2, Pages: 45-47(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.029
        摘要：目的:探讨鲜切饮片工艺的可行性,完善饮片操作规程。方法:以脂溶性和水溶性两类成分含量为指标,考察了直接鲜切饮片、水洗鲜切饮片和水蒸鲜切饮片对质量的影响。结果:最佳工艺是晾晒至67成干货时,抢水洗后,切薄片,阴干或低温烘干。结论:加工方式对饮片质量影响较大,采用鲜切饮片,可最大化保证其质量;鲜切饮片是产地适宜的加工方法,具有良好的推广前景。  
        关键词：甘肃丹参;鲜切饮片;加工方式   
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      • TIAN Neng,KONG Xiang-ying,WAN Rong,LIN Na,LI Li,CHEN Wei-heng
        Vol. 16, Issue 2, Pages: 48-51(2010)
        摘要：Objective: To investigate the effect of Jianpi Huoxue Decoction on the expression of vascular endothelial growth factor (VEGF) in the femoral head of chicken with Steroid-induced Osteonecrosis of the Femoral Head(SONFH), as well as to explore the mechanism of prevention and treatment for SONFH. Methods: The animal model of SONFH was established with injection of methylprednisolone in chest muscle of chickens. 64 Leghorn chickens were randomly divided into normal control group, model group, lovastatin group and Jianpi Huoxue group, 16 in each group. At 8th and 16th weeks, blood samples were obtained to determine the level of serum total cholesterol(CHO)and triglycerid (TG), then femoral heads of both sides were taken off to observe the histopathological changes with hematoxylin and eosin staining and the expression of VEGF with immunohistochemical method. Results: At 8th and 16th weeks, in model group, relatively typical pathological changes were observed in the femoral head, the ratio of empty lacuna and fat area increased obviously, Compared with normal group, the level of serum CHO and TG increased and the expression of VEGF decreased significantly(P<0.05); Both lovastatin and Jianpi Huoxue Decoction could improve the morphological changes of steroid-induced femoral head necrosis at different levels, increased the expression of VEGF, decreased the level of serum CHO and TG(Compared with model group, P<0.05). Conclusions: Jianpi Huoxue Decoction can up-regulate the expression of VEGF, this may be one of the mechanisms for improvement in microcirculation in femoral head and the preventive and therapeutic effect for SONFH.  
        关键词：steroil-induced osteonecrosis of the femoral head;Jianpi Huoxue;CHO;TG;VEGF   
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      • TIAN Neng~1,KONG Xiang-ying~1,WAN Rong~1,LIN Na~
        Vol. 16, Issue 2, Pages: 48-51(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.030
        摘要：Objective:To investigate the effect of Jianpi Huoxue Decoction on the expression of vascular endothelial growth factor（VEGF） in the femoral head of chicken with Steroid-induced Osteonecrosis of the Femoral Head（SONFH）,as well as to explore the mechanism of prevention and treatment for SONFH.Methods:The animal model of SONFH was established with injection of methylprednisolone in chest muscle of chickens.64 Leghorn chickens were randomly divided into normal control group,model group,lovastatin group and Jianpi Huoxue group,16 in each group.At 8th and 16th weeks,blood samples were obtained to determine the level of serum total cholesterol（CHO）and triglycerid（TG）,then femoral heads of both sides were taken off to observe the histopathological changes with hematoxylin and eosin staining and the expression of VEGF with immunohistochemical method.Results:At 8th and 16th weeks,in model group,relatively typical pathological changes were observed in the femoral head,the ratio of empty lacuna and fat area increased obviously,Compared with normal group,the level of serum CHO and TG increased and the expression of VEGF decreased significantly（P<0.05）;Both lovastatin and Jianpi Huoxue Decoction could improve the morphological changes of steroid-induced femoral head necrosis at different levels,increased the expression of VEGF,decreased the level of serum CHO and TG（Compared with model group,P<0.05）.Conclusions:Jianpi Huoxue Decoction can up-regulate the expression of VEGF,this may be one of the mechanisms for improvement in microcirculation in femoral head and the preventive and therapeutic effect for SONFH.  
        关键词：steroil-induced osteonecrosis of the femoral head;Jianpi Huoxue;CHO;TG;VEGF   
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      • LIU Jian-gang,LI Hao,YAO Ming-jiang,ZHAO Wen-ming
        Vol. 16, Issue 2, Pages: 52-55(2010)
        摘要：Objective: To observe the effects of Huannao Yicong Prescription(HYP) on learning and memory impairment,level of brain β-amyloid Protein (β-AP)and substance P(S.P)in aging rats. Methods: Morris water maze test was used to measure the spatial learning and memory of rats. 40 rats with learning and memory impairment were divided randomly into four groups (10 in each group):the model group (learning and memory impairment aged rats group),the high-dose HYP group (13.68 g crude drug/kg),the low-dose HYP group (6.84 g crude drug/kg),the positive control group (Hydrochloricdonepezil Tablets 0.45 mg/kg),besides,a normal aged rats group with 10 rats was set up,they were given tales doses of distilled water,all other groups were orally administered with drugs for 8 weeks. Then the spatial learning and memory of rats were measured again by Morris water maze. Rats were sacrificed after anesthesia and the brain homogenate was made for measuring the content of β-AP and S.P. The activity of superoxide dismutase (SOD) and the content of maleic dialdehyde (MDA) in serum was measured,the histomorphological changes in the brain were observed after hematoxylin eosin (HE) staining. Results: The latency and path length in water maze test in the model group were significantly prolonged. After 8 weeks of administration,the latency and path length in both of the HYP groups were significantly shortened than the model group,and the strategy in finding the platform was improved. Compared with the normal aged rats group,the content of MDA was significantly increased while the activity of SOD was significantly decreased in the model group. After 8 weeks of administration,compared with the model group,the level of β-AP was decreased while the S.P was increased,the activity of SOD was significantly increased(P<0.01)and the histomorphology in the brain was improved in the HYP groups. Conclusion: HYP can reduce the deposition of β-AP in the brain tissue,protect the integrity of histomorphology in the hippocampus,and thus improve the spatial learning and memory for aged rats.  
        关键词：Huannao Yicong Prescription;Alzheimers disease;learning and memory;Morris water maze test;β-amyloid protein;substance P   
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      • LIU Jian-gang~,LI Hao,YAO Ming-jiang,ZHAO Wen-ming
        Vol. 16, Issue 2, Pages: 52-55(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.031
        摘要：Objective:To observe the effects of Huannao Yicong Prescription（HYP） on learning and memory impairment,level of brain β-amyloid Protein（β-AP）and substance P（S.P）in aging rats.Methods: Morris water maze test was used to measure the spatial learning and memory of rats.40 rats with learning and memory impairment were divided randomly into four groups（10 in each group）:the model group（learning and memory impairment aged rats group）,the highdose HYP group（13.68 g crude drug/kg）,the low-dose HYP group（6.84 g crude drug/kg）,the positive control group（Hydrochloricdonepezil Tablets 0.45 mg/kg）,besides,a normal aged rats group with 10 rats was set up,they were given tales doses of distilled water,all other groups were orally administered with drugs for 8 weeks.Then the spatial learning and memory of rats were measured again by Morris water maze.Rats were sacrificed after anesthesia and the brain homogenate was made for measuring the content of β-AP and S.P.The activity of superoxide dismutase（SOD） and the content of maleic dialdehyde（MDA） in serum was measured,the histomorphological changes in the brain were observed after hematoxylin eosin（HE） staining.Results:The latency and path length in water maze test in the model group were significantly prolonged.After 8 weeks of administration,the latency and path length in both of the HYP groups were significantly shortened than the model group,and the strategy in finding the platform was improved.Compared with the normal aged rats group,the content of MDA was significantly increased while the activity of SOD was significantly decreased in the model group.After 8 weeks of administration,compared with the model group,the level of β-AP was decreased while the S.P was increased,the activity of SOD was significantly increased（P<0.01）and the histomorphology in the brain was improved in the HYP groups.Conclusion:HYP can reduce the deposition of β-AP in the brain tissue,protect the integrity of histomorphology in the hippocampus,and thus improve the spatial learning and memory for aged rats.  
        关键词：Huannao Yicong Prescription;Alzheimer’s disease;learning and memory;Morris water maze test;β-amyloid protein;substance P   
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      • YE Jin-xia,WANG Lan,YANG Bin,LIANG Ri|xin
        Vol. 16, Issue 2, Pages: 56-59(2010)
        摘要：Objective: To investigate the antioxidant effects of water extract of Carthamus tinctorius L. on injury of cardiomyocytes induced by hydrogen peroxide (H2O2) and to detect reactive oxygen species (ROS) for exploring the mechanisms. Methods: In cultured neonatal rat cardiomyocytes, the antioxidant effect of water extract of Carthamus tinctorius L. was investigated after H2O2 (1 mmol·L-1) induced damage. The spontaneous beating, the survival rate and release of LDH, MDA, SOD, NO, NOS, GSH-Px and XOD enzyme activity in supernatant were investigated, and cell suspension was collected for detecting reactive oxygen species (ROS) by electron spin resonance (ESR). Results: Preconditioning by water extract of Carthamus tinctorius L. increased the spontaneous beating and the survival rate, and decreased the release of LDH, MDA and SOD with the rise of SOD, NO, NOS and GSH-Px enzyme activity, while in the cell suspension ROS signal decreased significantly. Conclusion: Water extract of Carthamus tinctorius L. has antioxidative effect. The mechanisms are likely related with scavenging of free radicals, enhancing its clearance, enhancing endogenous antioxidant activity.  
        关键词：water extract of Carthamus tinctorius L.;cardiomyocytes;hydrogen peroxide(H2O2);electron spin resonance   
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      • YE Jin-xia~
        Vol. 16, Issue 2, Pages: 56-59(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.032
        摘要：Objective:To investigate the antioxidant effects of water extract of Carthamus tinctorius L.on injury of cardiomyocytes induced by hydrogen peroxide（H2O2） and to detect reactive oxygen species（ROS） for exploring the mechanisms.Methods:In cultured neonatal rat cardiomyocytes,the antioxidant effect of water extract of Carthamus tinctorius L.was investigated after H2O2(1 mmol·L-1) induced damage.The spontaneous beating,the survival rate and release of LDH,MDA,SOD,NO,NOS,GSH-Px and XOD enzyme activity in supernatant were investigated,and cell suspension was collected for detecting reactive oxygen species（ROS） by electron spin resonance（ESR）.Results: Preconditioning by water extract of Carthamus tinctorius L.increased the spontaneous beating and the survival rate,and decreased the release of LDH,MDA and SOD with the rise of SOD,NO,NOS and GSH-Px enzyme activity,while in the cell suspension ROS signal decreased significantly.Conclusion: Water extract of Carthamus tinctorius L.has antioxidative effect.The mechanisms are likely related with scavenging of free radicals,enhancing its clearance,enhancing endogenous antioxidant activity.  
        关键词：water extract of Carthamus tinctorius L.;cardiomyocytes;hydrogen peroxide（H2O2）;electron spin resonance   
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      • YU Xiao,WU Da-zheng
        Vol. 16, Issue 2, Pages: 60-64(2010)
        摘要：Objective: To investigate the protective effects of water extract of Coptis chinensis and Evodia rutaecarpa on ethanol-induced acute gastric lesions in rats. Methods: In this study, animals were intraperitoneally pretreated with vehicle (normal saline) or Coptis chinensis and Evodia rutaecarpa water extract at 1050 (6 ∶1), 1800 (6 ∶6), 1050 (1 ∶6) mg·kg-1. Three hours later, gastric lesions were induced by topical application of 50% ethanol for 10 min. The length index of gastric lesions were determined by morphological analysis in a gastric intubatton model. The rat gastric transmucosal potential difference (PD) was recorded continuously by ex-vivo chamber technique. The pH value and H+ content of gastric perfusate were mesured by pH meter and pH-stat technique, respectively. Results: Compared with control, Coptis chinensis and Evodia rutaecarpa water extract (1 ∶6) accelerated PD recovery and reduced gastric morphologic lesions. Coptis chinensis and Evodia rutaecarpa water extract (1 ∶6) elevated H+ content and reduced pH value. Coptis chinensis and Evodia rutaecarpa water extract (6 ∶6) reduced gastric morphologic lesions. Conclusions: Coptis chinensis and E. rutaecarpa water extract (1 ∶6) showed effectively protective actions on ethanol-induced acute gastric lesions in rats, and the gastroprotective mechanisms maybe due to the strengthening action on gastric mucosal lining and the inhibition of acid backdiffusion. This investigation also indicated that E. rutaecarpa maybe have a critical role in gastroprotective effect.  
        关键词：Coptis chinensis;Evodia rutaecarpa;gastric potential difference;gastric lesion index;rat   
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      • YU Xiao~
        Vol. 16, Issue 2, Pages: 60-64(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.033
        摘要：Objective:To investigate the protective effects of water extract of Coptis chinensis and Evodia rutaecarpa on ethanol-induced acute gastric lesions in rats.Methods:In this study,animals were intraperitoneally pretreated with vehicle（normal saline） or Coptis chinensis and Evodia rutaecarpa water extract at 1050（6∶1）,1800（6∶6）,1050（1∶6） mg·kg-1.Three hours later,gastric lesions were induced by topical application of 50% ethanol for 10 min.The length index of gastric lesions were determined by morphological analysis in a gastric intubatton model.The rat gastric transmucosal potential difference（PD） was recorded continuously by ex-vivo chamber technique.The pH value and H+ content of gastric perfusate were mesured by pH meter and pH-stat technique,respectively.Results:Compared with control,Coptis chinensis and Evodia rutaecarpa water extract（1∶6） accelerated PD recovery and reduced gastric morphologic lesions.Coptis chinensis and Evodia rutaecarpa water extract（1∶6） elevated H+ content and reduced pH value.Coptis chinensis and Evodia rutaecarpa water extract（6∶6） reduced gastric morphologic lesions.Conclusions:Coptis chinensis and E.rutaecarpa water extract（1∶6） showed effectively protective actions on ethanol-induced acute gastric lesions in rats,and the gastroprotective mechanisms maybe due to the strengthening action on gastric mucosal lining and the inhibition of acid backdiffusion.This investigation also indicated that E.rutaecarpa maybe have a critical role in gastroprotective effect.  
        关键词：Coptis chinensis;Evodia rutaecarpa;gastric potential difference;gastric lesion index;rat   
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      • LI Shu-wen,WU Qing-he,HUANG Ping,CAO Hong-ying
        Vol. 16, Issue 2, Pages: 64-67(2010)
        摘要：Objective: To explore the mechanism for kidney deficiency and diuresis by studying the action of adenine to ALD synthase. Methods: The contents of Cort,ALD in blood were determined and the mRNA expression of CYP11B2 was evaluated with ELISA and RT-PCR in model group and control group. Results: Compared with control group, adenine could remarkedly decrease the contents of Cort,ALD in rat blood; and downregulate the mRNA expression of CYP11B2. Conclusion: Adenine can decrease the contents of Cort,ALD in blood and downregulate the mRNA expression of CYP11B2 in rats. It can decrease the synthesis of ALD by adjusting the ALD synthase. The results may expound the mechanism of kidney deficiency and diuresis induced by adenine from the angle of ALD synthase approach.  
        关键词：adenine;kidney deficiency and diuresis;ALD synthase   
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      • LI Shu-wen~
        Vol. 16, Issue 2, Pages: 64-67(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.034
        摘要：Objective:To explore the mechanism for kidney deficiency and diuresis by studying the action of adenine to ALD synthase.Methods:The contents of Cort,ALD in blood were determined and the mRNA expression of CYP11B2 was evaluated with ELISA and RT-PCR in model group and control group.Results:Compared with control group,adenine could remarkedly decrease the contents of Cort,ALD in rat blood;and downregulate the mRNA expression of CYP11B2.Conclusion:Adenine can decrease the contents of Cort,ALD in blood and downregulate the mRNA expression of CYP11B2 in rats.It can decrease the synthesis of ALD by adjusting the ALD synthase.The results may expound the mechanism of kidney deficiency and diuresis induced by adenine from the angle of ALD synthase approach.  
        关键词：adenine;kidney deficiency and diuresis;ALD synthase   
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      • Vol. 16, Issue 2, Pages: 68-70(2010)
        摘要：Objective: To investigate the antioxidant effects of water extract of Carthamus tinctorius L. on injury of cardiomyocytes induced by hydrogen peroxide (H2O2) and to detect reactive oxygen species (ROS) for exploring the mechanisms. Methods: In cultured neonatal rat cardiomyocytes, the antioxidant effect of water extract of Carthamus tinctorius L. was investigated after H2O2 (1 mmol·L-1) induced damage. The spontaneous beating, the survival rate and release of LDH, MDA, SOD, NO, NOS, GSH-Px and XOD enzyme activity in supernatant were investigated, and cell suspension was collected for detecting reactive oxygen species (ROS) by electron spin resonance (ESR). Results: Preconditioning by water extract of Carthamus tinctorius L. increased the spontaneous beating and the survival rate, and decreased the release of LDH, MDA and SOD with the rise of SOD, NO, NOS and GSH-Px enzyme activity, while in the cell suspension ROS signal decreased significantly. Conclusion: Water extract of Carthamus tinctorius L. has antioxidative effect. The mechanisms are likely related with scavenging of free radicals, enhancing its clearance, enhancing endogenous antioxidant activity.  
          
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      • Vol. 16, Issue 2, Pages: 68-70(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.035
        摘要：目的:探讨寒热中药成分对瞬变感受器离子通道蛋白（TRP）家族中薄荷醇受体离子通道蛋白（TRPM8）功能的影响。方法:以薄荷醇为通道激动剂,采用共聚焦显微成像法在体外观察原代培养背根神经节（DRG）神经元胞内[Ca2+]i的变化。结果:寒性中药的成分黄芩甙和大黄素均可显著上调TRPM8通道蛋白的功能;热性中药的成分桂皮醛可显著下调TR-PM8通道蛋白的功能,热性成分吴茱萸碱对TRPM8通道蛋白功能虽未见显著影响,但有下调趋势。结论:寒热性中药成分对TRPM8功能的调节可能与中药的寒热药性相关,这可能是寒热性中药临床上发挥寒热调节作用的分子机制之一。  
        关键词：中药药性;瞬变感受器离子通道蛋白;背根神经节神经元   
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      • Vol. 16, Issue 2, Pages: 71-72(2010)
        摘要：Objective: To investigate the protective effects of water extract of Coptis chinensis and Evodia rutaecarpa on ethanol-induced acute gastric lesions in rats. Methods: In this study, animals were intraperitoneally pretreated with vehicle (normal saline) or Coptis chinensis and Evodia rutaecarpa water extract at 1050 (6 ∶1), 1800 (6 ∶6), 1050 (1 ∶6) mg·kg-1. Three hours later, gastric lesions were induced by topical application of 50% ethanol for 10 min. The length index of gastric lesions were determined by morphological analysis in a gastric intubatton model. The rat gastric transmucosal potential difference (PD) was recorded continuously by ex-vivo chamber technique. The pH value and H+ content of gastric perfusate were mesured by pH meter and pH-stat technique, respectively. Results: Compared with control, Coptis chinensis and Evodia rutaecarpa water extract (1 ∶6) accelerated PD recovery and reduced gastric morphologic lesions. Coptis chinensis and Evodia rutaecarpa water extract (1 ∶6) elevated H+ content and reduced pH value. Coptis chinensis and Evodia rutaecarpa water extract (6 ∶6) reduced gastric morphologic lesions. Conclusions: Coptis chinensis and E. rutaecarpa water extract (1 ∶6) showed effectively protective actions on ethanol-induced acute gastric lesions in rats, and the gastroprotective mechanisms maybe due to the strengthening action on gastric mucosal lining and the inhibition of acid backdiffusion. This investigation also indicated that E. rutaecarpa maybe have a critical role in gastroprotective effect.  
          
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      • Vol. 16, Issue 2, Pages: 71-72(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.036
        摘要：目的:探讨不同类型醋炮制延胡索镇痛作用;对不同类型醋进行安全性比较,为筛选炮制辅料用醋提供依据。方法:采用前实验筛选出的6种醋炮制延胡索,比较其镇痛作用;采用不同类型的醋比较对小鼠单次ig的毒性。结果:各醋炮制品组与对照组比较均有明显的镇痛作用,组间比较无明显差异;小鼠ig食用米醋未见毒副反应,工业醋酸及用食用醋酸配制的白醋,各组小鼠ig醋72 h内均出现不同程度异常反应及死亡。结论:筛选的6个品牌醋可作为延胡索炮制辅料用醋,6种醋炮制延胡索镇痛作用无显著差异;工业醋酸及用食用醋酸配制的白醋较发酵米醋安全性差。  
        关键词：不同类型醋;炮制延胡索;镇痛;毒副反应   
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      • WANG Huan,TANG Yu-ping,GUO Jian-ming,Ding An-wei,LI Wei-xia,JIANG Wei,DUAN Jin-ao
        Vol. 16, Issue 2, Pages: 73-77(2010)
        摘要：Objective: To observe the effect of Radix Angelica and Rhizoma Ligusticum with different proportions (1 ∶0,2 ∶1,1.5 ∶1,1 ∶1,1 ∶1.5,1 ∶2,0 ∶1) extracted by different methods (by water、by ethanol、firstly by alcohol then by water) on platelet aggregation and prothrombin time (PT), thrombin time (TT). Methods: Take blood from carotid artery of rabbit and measure the rate of platelet aggregation induced by ADP and PT, TT to compare their different effects. Results: Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods showed certain effects of anti-platelet aggregation and antithrombin (P<0.01 or P<0.05). The effect on PT and TT enhanced along with the ratio of Radix Angelica gradually increases; The platelet aggregation effect was the weakest While the ratio of Danggui-Chuanxiong was 1.5 ∶1. Compared with other extracts, the solution extracted by water showed a weaker effect of platelet aggregation, while solution extracted by ethanol showed a stronger effect on PT than TT. Conclusion: The different traditional efficacy of Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods may be related with their different effects of anti-platelet aggregation and antithrombin, their intrinsic material basis and relevant mechanism are worthy of further study.  
        关键词：Radix Angelica;Rhizoma ligusticum;crude drug pair;proportion and preparation method;platelet aggregation;prothrombin time;thrombin time   
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      • WANG Huan,TANG Yu-ping~,GUO Jian-ming,Ding An-wei~,LI Wei-xia,JIANG Wei,DUAN Jin-ao
        Vol. 16, Issue 2, Pages: 73-77(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.037
        摘要：Objective:To observe the effect of Radix Angelica and Rhizoma Ligusticum with different proportions（1∶0,2∶1,1.5∶1,1∶1,1∶1.5,1∶2,0∶1） extracted by different methods（by water、by ethanol、firstly by alcohol then by water） on platelet aggregation and prothrombin time（PT）,thrombin time（TT）.Methods: Take blood from carotid artery of rabbit and measure the rate of platelet aggregation induced by ADP and PT,TT to compare their different effects.Results: Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods showed certain effects of anti-platelet aggregation and antithrombin（P<0.01 or P<0.05）.The effect on PT and TT enhanced along with the ratio of Radix Angelica gradually increases;The platelet aggregation effect was the weakest While the ratio of Danggui-Chuanxiong was 1.5∶1.Compared with other extracts,the solution extracted by water showed a weaker effect of platelet aggregation,while solution extracted by ethanol showed a stronger effect on PT than TT.Conclusion: The different traditional efficacy of Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods may be related with their different effects of anti-platelet aggregation and antithrombin,their intrinsic material basis and relevant mechanism are worthy of further study.  
        关键词：Radix Angelica;Rhizoma ligusticum;crude drug pair;proportion and preparation method;platelet aggregation;prothrombin time;thrombin time   
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      • REN Jun-guo,MA Xiao-bin,LIN Cheng-ren,WANG Yang-hui,LI Hong-hai,WANG Min,LI Jun-mei,LIU Jian-xun
        Vol. 16, Issue 2, Pages: 78-80(2010)
        摘要：Objective: To investigate protective effect of Jiawei Shengmaisan against acute myocardial ischemia/reperfusion injury in rats. Methods: The acute myocardial ischemia/reperfusion injury in rats was induced by ligating the anterior descending coronary artery in SD rats and administered different dosage jiawei shengmaisan decotion into duodenum, myocardial infarction degree(MID) was calculated by detcting myocardial infarction area with nitroblue tetrazolium(NBT) assay,SOD activities and MDA、NO contents were detected with biochemical assay. Results: Jiawei shengmaisan can significantly decreased MID and MDA contents in serum,increased SOD activities and NO contents in serum. Conclusions: The results showed that Jiawei shengmaisan has protective effect against acute myocardial ischemia/reperfusion injury in rats,which may be relate to its antioxygen action and NO.  
        关键词：Jiawei Shengmaisan;Shengmaisan;myocardial ischemia/reperfusion injury   
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      • REN Jun-guo,MA Xiao-bin,LIN Cheng-ren,WANG Yang-hui,LI Hong-hai,WANG Min,LI Jun-mei,LIU Jian-xun
        Vol. 16, Issue 2, Pages: 78-80(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.038
        摘要：Objective:To investigate protective effect of Jiawei Shengmaisan against acute myocardial ischemia/reperfusion injury in rats.Methods:The acute myocardial ischemia/reperfusion injury in rats was induced by ligating the anterior descending coronary artery in SD rats and administered different dosage jiawei shengmaisan decotion into duodenum,myocardial infarction degree（MID） was calculated by detcting myocardial infarction area with nitroblue tetrazolium（NBT） assay,SOD activities and MDA、NO contents were detected with biochemical assay.Results: Jiawei shengmaisan can significantly decreased MID and MDA contents in serum,increased SOD activities and NO contents in serum.Conclusions: The results showed that Jiawei shengmaisan has protective effect against acute myocardial ischemia/reperfusion injury in rats,which may be relate to its antioxygen action and NO.  
        关键词：Jiawei Shengmaisan;Shengmaisan;myocardial ischemia/reperfusion injury   
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      • ZHANG Jun-li,JIA Cheng,YUAN Bo,WANG Yang,CHENG Jia-yi
        Vol. 16, Issue 2, Pages: 81-83(2010)
        摘要：Objective: To study the effect and mechanism of Radix Notoginseng compound on inhibition of thrombosis. Methods: The rat thrombosis nodel was induced by electric stimulation. The influences of Radix Notoginseng Compound on thrombus formation and hemorheological parameters were investigated. The rat model of blood stasis was established by combined application of epinephrine with ice bath. The influences of the compound on thrombixin A2 and prostaglandin I2 were observed for the model. Results: Compared with the control group,each dose of Radix Notoginseng Compound and aspirin tablet could extend the latency period for thrombus formation, and decrease blood viscosity. The compound could significantly increase the plasma levels of 6-Keto-PGF1α and the ratio of 6-Keto-PGF1α/TXB2 for the blood stasis rats (both P<0.01). The level of TXB2 was significantly decreased (P<0.05). Conclusion: Radix Notoginseng compound has inhibitive effect on thrombosis,the mechanism of action is closely related with decrease in blood viscosity and chang in the PGI2/TXA2 ratios.  
        关键词：Radix notoginseng;thrombus;hemorhelogy;TXA2;PGI2   
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      • ZHANG Jun-li~1,JIA Cheng~2,YUAN Bo~1,WANG Yang~1,CHENG Jia-yi~
        Vol. 16, Issue 2, Pages: 81-83(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.039
        摘要：Objective:To study the effect and mechanism of Radix Notoginseng compound on inhibition of thrombosis.Methods: The rat thrombosis nodel was induced by electric stimulation.The influences of Radix Notoginseng Compound on thrombus formation and hemorheological parameters were investigated.The rat model of blood stasis was established by combined application of epinephrine with ice bath.The influences of the compound on thrombixin A2 and prostaglandin I2 were observed for the model.Results: Compared with the control group,each dose of Radix Notoginseng Compound and aspirin tablet could extend the latency period for thrombus formation,and decrease blood viscosity.The compound could significantly increase the plasma levels of 6-Keto-PGF1α and the ratio of 6-Keto-PGF1α/TXB2 for the blood stasis rats（both P<0.01）.The level of TXB2 was significantly decreased（P<0.05）.Conclusion:Radix Notoginseng compound has inhibitive effect on thrombosis,the mechanism of action is closely related with decrease in blood viscosity and chang in the PGI2/TXA2 ratios.  
        关键词：Radix notoginseng;thrombus;hemorhelogy;TXA2;PGI2   
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      • SHEN Ye-hua,FU Jie,LIU Lu-ming,SHEN Jin,LI Dong-liang
        Vol. 16, Issue 2, Pages: 84-86(2010)
        摘要：Objective: To explore the effect of the herbal decoction Qingyi Huaji formula (QYHJ) combined with gemcitabine on apoptosis and expression of Bcl-2 protein of human pancreatic cancer SW1990 in vivo. Methods: Tumor-burdened nude mice were randomized into control group, Chemo group, groups of Chemo plus QYHJ at different doses. After treatment, inhibiting rates of tumor growth were calculated. Cell apoptosis of each group was extracted by flow cytometry(FCM). Expression of Bcl-2 protein was learned by method of immunohistochemistry. Result: Tumor weight in the Chemo plus QYHJ groups at different doses was significantly lower than that in the Chemo group (P<0.05). Compared to the Chemo group, cell apoptosis rate in the Chemo plus QYHJ groups at middle dose was higher (P<0.05), while the expression of Bcl-2 was lower (P<0.05). Conclusion: QYHJ formula may have synergtic action on Gemcitabine, to inhibit the proliferation of human pancreatic cancer SW1990 in vivo, possibly by inhibiting expression of Bcl-2 protein and inducing cell apoptosis.  
        关键词：Qingyi Huaji Formula;pancreatic cancer;apoptosis;Bcl-2 protein   
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      • SHEN Ye-hua~1,FU Jie~1,LIU Lu-ming~
        Vol. 16, Issue 2, Pages: 84-86(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.047
        摘要：Objective:To explore the effect of the herbal decoction Qingyi Huaji formula（QYHJ） combined with gemcitabine on apoptosis and expression of Bcl-2 protein of human pancreatic cancer SW1990 in vivo.Methods: Tumor-burdened nude mice were randomized into control group,Chemo group,groups of Chemo plus QYHJ at different doses.After treatment,inhibiting rates of tumor growth were calculated.Cell apoptosis of each group was extracted by flow cytometry（FCM）.Expression of Bcl-2 protein was learned by method of immunohistochemistry.Result: Tumor weight in the Chemo plus QYHJ groups at different doses was significantly lower than that in the Chemo group（P<0.05）.Compared to the Chemo group,cell apoptosis rate in the Chemo plus QYHJ groups at middle dose was higher（P<0.05）,while the expression of Bcl-2 was lower（P<0.05）.Conclusion: QYHJ formula may have synergtic action on Gemcitabine,to inhibit the proliferation of human pancreatic cancer SW1990 in vivo,possibly by inhibiting expression of Bcl-2 protein and inducing cell apoptosis.  
        关键词：Qingyi Huaji Formula;pancreatic cancer;apoptosis;Bcl-2 protein   
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      • Vol. 16, Issue 2, Pages: 87-89(2010)
        摘要：Objective: To investigate protective effect of Jiawei Shengmaisan against acute myocardial ischemia/reperfusion injury in rats. Methods: The acute myocardial ischemia/reperfusion injury in rats was induced by ligating the anterior descending coronary artery in SD rats and administered different dosage jiawei shengmaisan decotion into duodenum, myocardial infarction degree(MID) was calculated by detcting myocardial infarction area with nitroblue tetrazolium(NBT) assay,SOD activities and MDA、NO contents were detected with biochemical assay. Results: Jiawei shengmaisan can significantly decreased MID and MDA contents in serum,increased SOD activities and NO contents in serum. Conclusions: The results showed that Jiawei shengmaisan has protective effect against acute myocardial ischemia/reperfusion injury in rats,which may be relate to its antioxygen action and NO.  
          
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      • Vol. 16, Issue 2, Pages: 87-89(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.040
        摘要：目的:对治疗痛风病疗效确切的院内制剂痛风灵微丸的处方结构进行优化研究,为其临床前研究提供疗效可靠的优化处方,探索中药复方定量优化方法。方法:运用均匀设计改良法,以特异的药效学指标为评价指标,进行处方最佳配伍和最佳剂量配比的研究。结果:以均匀设计法得出痛风灵微丸的最佳配伍和最佳剂量配比的处方值。结论:该实验研究将拆方分析方法与最佳剂量配比研究结合起来,得到了比实践少1味药物的结论,并推荐其用于新药开发研究。  
        关键词：痛风灵;微丸;均匀设计;药物组合优化   
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      • Vol. 16, Issue 2, Pages: 90-93(2010)
        摘要：Objective: To study the effect and mechanism of Radix Notoginseng compound on inhibition of thrombosis. Methods: The rat thrombosis nodel was induced by electric stimulation. The influences of Radix Notoginseng Compound on thrombus formation and hemorheological parameters were investigated. The rat model of blood stasis was established by combined application of epinephrine with ice bath. The influences of the compound on thrombixin A2 and prostaglandin I2 were observed for the model. Results: Compared with the control group,each dose of Radix Notoginseng Compound and aspirin tablet could extend the latency period for thrombus formation, and decrease blood viscosity. The compound could significantly increase the plasma levels of 6-Keto-PGF1α and the ratio of 6-Keto-PGF1α/TXB2 for the blood stasis rats (both P<0.01). The level of TXB2 was significantly decreased (P<0.05). Conclusion: Radix Notoginseng compound has inhibitive effect on thrombosis,the mechanism of action is closely related with decrease in blood viscosity and chang in the PGI2/TXA2 ratios.  
          
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      • Vol. 16, Issue 2, Pages: 90-93(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.041
        摘要：目的:探讨健脾补肾方防治疗激素性股骨头坏死的作用机制。方法:采用激素性股骨头坏死（SINFH）模型,以蒸馏水作为空白对照,观察各实验组治疗后的病理形态学变化,并对各实验组血脂指标进行动态监测。结果:健脾补肾方能明显改善家兔SINFH模型组织形态学,12周时股骨头标本HE染色证明各治疗组空骨陷窝率和髓腔脂肪面积与模型组比较差异显著或非常显著（P<0.05或P<0.01）;健脾补肾方可纠正激素造成的脂质代谢紊乱状况,各治疗组与模型组比较,在治疗后的4周和（或）8周和（或）12周,各项血脂指标差异显著或非常显著（P<0.05或P<0.01）。结论:健脾补肾方对SINFH的防治机理可能与纠正激素造成的脂质代谢紊乱有关。  
        关键词：健脾补肾方;股骨头坏死;激素;脂质代谢   
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      • Vol. 16, Issue 2, Pages: 94-95(2010)
        摘要：Objective: To explore the effect of the herbal decoction Qingyi Huaji formula (QYHJ) combined with gemcitabine on apoptosis and expression of Bcl-2 protein of human pancreatic cancer SW1990 in vivo. Methods: Tumor-burdened nude mice were randomized into control group, Chemo group, groups of Chemo plus QYHJ at different doses. After treatment, inhibiting rates of tumor growth were calculated. Cell apoptosis of each group was extracted by flow cytometry(FCM). Expression of Bcl-2 protein was learned by method of immunohistochemistry. Result: Tumor weight in the Chemo plus QYHJ groups at different doses was significantly lower than that in the Chemo group (P<0.05). Compared to the Chemo group, cell apoptosis rate in the Chemo plus QYHJ groups at middle dose was higher (P<0.05), while the expression of Bcl-2 was lower (P<0.05). Conclusion: QYHJ formula may have synergtic action on Gemcitabine, to inhibit the proliferation of human pancreatic cancer SW1990 in vivo, possibly by inhibiting expression of Bcl-2 protein and inducing cell apoptosis.  
          
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      • Vol. 16, Issue 2, Pages: 94(2010)
        摘要：This article discusses the confused situations when the traditional Chinese medicine material names are translated from Chinese into English, the wrong applications of the original Latin names, the inaccurate expressions of English words, the unscientific translations of Pinyin names.  
        关键词：latin name;traditional chinese medicine material name;english name;pinyin;translation   
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      • Vol. 16, Issue 2, Pages: 94-95(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.021
        摘要：目的:观察茶多酚对小鼠高脂血症和脂肪肝的预防作用。方法:实验小鼠分为正常组、高脂组、脂必妥组和茶多酚组。喂养6周后,测定血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇脂（LDL-c）、高密度脂蛋白胆固醇脂（HDL-c）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活力,测定肝脏匀浆液的丙二醛（MDA）含量并观察肝脏冰冻切片脂肪染色。结果:茶多酚对高脂血症小鼠血清TC,TG,LDL-c,ALT和AST值均有显著降低,HDL-C有明显升高,肝脏组织中MDA含量也有所降低,肝脏冰冻切片的脂肪染色显示茶多酚明显减少肝脏中脂肪含量。结论:茶多酚对高脂血症和脂肪肝的形成有明显的预防作用。  
        关键词：茶多酚;高脂血症;脂肪肝   
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      • LIU Hong-jun,YU Hai-bin,GAO Yuan,ZHU Ming-jun,WANG Yong-xia,LI Si-si
        Vol. 16, Issue 2, Pages: 96-100(2010)
        摘要：Objective: To investigate the effect of Cyclovirobuxine D(CVB-D) on action potentials in isolated guinea pig ventricular myocytes. Methods: The single myocytes were enzymatically obtained from the guinea pig hearts and the action potentials were examined by whole-cell patchclamp technique. Results: 4×10-4 g/L CVB-D decreased action potential amptitude(APA)from 113.3±6.3 mV to 110.2±6.4 mV(n=6, P<0.01), prolonged action potential duration of repolarization 50%(APD50)from 616.3±68.0 ms to 657.4±79.5 ms(n=6, P<0.01),prolonged action potential duration of repolarization 90%(APD90)from 633.5±71.2 ms to 669.2±74.4 ms(n=6, P<0.01), Prolonged action potential duration of repolarization 50% to repolarization 90%(APD50-90)from 15.9±6.0 ms to 20.1±5.2 ms(n=6, P<0.01),and decreased decay slope of phase 3 (V3) from -2.6±0.8 V/s to -2.4±0.7 V/s(n=6, P<0.01).Compared with the baseline,4×10-3 g/L CVB-D makes the APD50-90 pass to increase trend gradually,but the variety trend of the V3 decrease gradually, other index sign varieties are consistent. Conclusion: CVB-D decreased APA and V3, and prolonged APD50,APD90,APD50-90,which may be its mechanism of positive inotropic effect and antiarrhythmic effect.  
        关键词：cyclovirobuxine D;action potential;patch clamp;arrhythmia   
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      • LIU Hong-jun~
        Vol. 16, Issue 2, Pages: 96-100(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.022
        摘要：Objective:To investigate the effect of Cyclovirobuxine D（CVB-D） on action potentials in isolated guinea pig ventricular myocytes.Methods:The single myocytes were enzymatically obtained from the guinea pig hearts and the action potentials were examined by whole-cell patchclamp technique.Results:4×10-4 g/L CVB-D decreased action potential amptitude（APA）from 113.3±6.3 mV to 110.2±6.4 mV（n=6,P<0.01）,prolonged action potential duration of repolarization 50%(APD50)from 616.3±68.0 ms to 657.4±79.5 ms（n=6,P<0.01）,prolonged action potential duration of repolarization 90%(APD90)from 633.5±71.2 ms to 669.2±74.4 ms（n=6,P<0.01）,Prolonged action potential duration of repolarization 50% to repolarization 90%(APD50-90)from 15.9±6.0 ms to 20.1±5.2 ms（n=6,P<0.01）,and decreased decay slope of phase 3（V3） from-2.6±0.8 V/s to-2.4±0.7 V/s（n=6,P<0.01）.Compared with the baseline,4×10-3 g/L CVB-D makes the APD50-90 pass to increase trend gradually,but the variety trend of the V3 decrease gradually,other index sign varieties are consistent.Conclusion:CVB-D decreased APA and V3,and prolonged APD50,APD90,APD50-90,which may be its mechanism of positive inotropic effect and antiarrhythmic effect.  
        关键词：cyclovirobuxine D;action potential;patch clamp;arrhythmia   
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      • WANG Lian-rong~
        Vol. 16, Issue 2, Pages: 100-103(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.023
        摘要：Objective:To observe the effect of Da-Fang-Feng-Tang（DFFT） in treating of Rheumatoid Arthritis（RA）.Methods: The animal model of type Ⅱ collagen induced arthritis was used.Granules of the DFFT extract were administered by gastric gavage,studying in genetics,immunology,physiology and clinical.Results: The results indicated a higher onset rate,a severer redness and swelling for arthritic limb and a longer time of clinic manifestation in contract group than treated group,and the IgG level in sera was significantly increased compared contract group to treated group（P<0.05）.The gene express of IL-1,TNF-α and IFN-γ were higher in contract group.Conclusion: DFFT express obviously suppressive function on model of type Ⅱ collagen induced arthritis in NIH mouse,and support the belief that DFFT is effective in treating human RA.  
        关键词：Da-Fang-Feng-Tang;rheumatoid arthritis;animal model   
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      • WANG Lian-rong,WU Wei-kang
        Vol. 16, Issue 2, Pages: 100-103(2010)
        摘要：Objective: To observe the effect of Da-Fang-Feng-Tang(DFFT) in treating of Rheumatoid Arthritis(RA). Methods : The animal model of type Ⅱ collagen induced arthritis was used. Granules of the DFFT extract were administered by gastric gavage, studying in genetics, immunology, physiology and clinical. Results : The results indicated a higher onset rate, a severer redness and swelling for arthritic limb and a longer time of clinic manifestation in contract group than treated group, and the IgG level in sera was significantly increased compared contract group to treated group(P<0.05). The gene express of IL-1, TNF-α and IFN-γ were higher in contract group. Conclusion : DFFT express obviously suppressive function on model of type Ⅱ collagen induced arthritis in NIH mouse, and support the belief that DFFT is effective in treating human RA.  
        关键词：Da-Fang-Feng-Tang;rheumatoid arthritis;animal model   
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      • YANG Yao-fang,SHEN Xiao-ying,QIAN Wei,QIAN Qing-qing,JI Min-chun
        Vol. 16, Issue 2, Pages: 104-106(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.024
        摘要：Objective:To study the hypoglycemic effect of Dahuang Zhechong Pill on alloxan-induced diabetic mice.Methods: We made up animal models of diabetic mice by intraperitoneal injection of 90 mg·kg-1 alloxan,the levels of glycated hemoglobin and lipids levels were measured.Mice were weighed before and after models made up.We anatomically separated the heart,liver,spleen,lung,kidney and thymus of the mice,weighed the organs and calculated the organ coefficient.Results: The level of blood glucose,glycated hemoglobin were increased,body weight and the weights of spleen,thymus were reduced after the models made up by introperitoneal injecting alloxan(90 mg·kg-1)on mice.Dahuang Zhechong Pill could reduce the level of blood glucose,glycated hemoglobin,increase level of high-density lipoprotein and improved the weight loss of the alloxan-induced diabetic mice,and increase the weights of spleen and thymus of immune organs.Conclusion: Dahuang Zhechong Pill has hypoglycemic effect on diabetic mice.  
        关键词：Dahuang Zhechong Pill;diabetic model;alloxan;hypoglycemic activity   
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      • YANG Yao-fang,SHEN Xiao-ying,QIAN Wei,QIAN Qing-qing,JI Min-chun
        Vol. 16, Issue 2, Pages: 104-106(2010)
        摘要：Objective: To study the hypoglycemic effect of Dahuang Zhechong Pill on alloxan-induced diabetic mice. Methods: We made up animal models of diabetic mice by intraperitoneal injection of 90 mg·kg-1 alloxan, the levels of glycated hemoglobin and lipids levels were measured. Mice were weighed before and after models made up. We anatomically separated the heart, liver, spleen, lung, kidney and thymus of the mice, weighed the organs and calculated the organ coefficient. Results: The level of blood glucose, glycated hemoglobin were increased, body weight and the weights of spleen, thymus were reduced after the models made up by introperitoneal injecting alloxan(90 mg·kg-1)on mice. Dahuang Zhechong Pill could reduce the level of blood glucose, glycated hemoglobin, increase level of high-density lipoprotein and improved the weight loss of the alloxan-induced diabetic mice, and increase the weights of spleen and thymus of immune organs. Conclusion: Dahuang Zhechong Pill has hypoglycemic effect on diabetic mice.  
        关键词：Dahuang Zhechong Pill;diabetic model;alloxan;hypoglycemic activity   
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      • ZENG Xiao-hui,CHEN Yu-xing,ZHAO Zi-ming,HUANG Xue-jun,DU Tie-liang
        Vol. 16, Issue 2, Pages: 107-110(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.025
        摘要：Objective:To study the pharmacokinetics of curcumin microcystin in rats.Methods: The microcystin was orally administrated.After carotid artery cannulation,blood samples were collected after administration of 0.08,0.25,0.5,0.75,1,2,3,4,5.5 h,and tested by HPLC.Results: The various doses of curcumin concentration-time curve is bimodal;Cmax1 and Cmax2 of each dose group are as follows: high-dose（0.90,1.20 mg/L）,medium dose（0.43,0.46 mg/L）,low dose（3.16,1.31 mg/L）; AUC are 3.79,2.60,5.92 mg·h/L respectively.Cmax and AUC of low dose are greater than that of the high and medium doses.Conclusion: The Pharmacokinetics of curcumin were nonlinear process in rat,there may be the phenomenon of enterohepatic circulation.  
        关键词：Curcumin Microcystin;pharmacokinetics;rat   
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      • ZENG Xiao-hui,CHEN Yu-xing,ZHAO Zi-ming,HUANG Xue-jun,DU Tie-liang
        Vol. 16, Issue 2, Pages: 107-110(2010)
        摘要：Objective: To study the pharmacokinetics of curcumin microcystin in rats. Methods: The microcystin was orally administrated. After carotid artery cannulation, blood samples were collected after administration of 0.08, 0.25, 0.5, 0.75, 1, 2, 3, 4, 5.5 h, and tested by HPLC. Results: The various doses of curcumin concentration-time curve is bimodal; Cmax1 and Cmax2 of each dose group are as follows: high-dose(0.90, 1.20 mg/L), medium dose(0.43, 0.46 mg/L), low dose(3.16, 1.31 mg/L); AUC are 3.79, 2.60, 5.92 mg·h/L respectively. Cmax and AUC of low dose are greater than that of the high and medium doses. Conclusion: The Pharmacokinetics of curcumin were nonlinear process in rat, there may be the phenomenon of enterohepatic circulation.  
        关键词：Curcumin Microcystin;pharmacokinetics;rat   
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      • Vol. 16, Issue 2, Pages: 110-112(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.026
        摘要：目的:探讨复方丹参注射液Ⅱ的制备及其指标成分丹酚酸B在大鼠体内的含量测定方法。方法:通过羟丙基-β-环糊精包合增加降香挥发油溶解性;用HPLC法测定主成分丹酚酸B在大鼠体内的含量。结果:成功制备复方丹参注射液Ⅱ,建立注射液中指标成分丹酚酸B在大鼠体内的HPLC测定方法。结论:复方丹参注射液Ⅱ的制备工艺可行,指标成分丹酚酸B在大鼠体内的含量测定方法准确可靠。  
        关键词：复方丹参注射液;丹酚酸B;高效液相色谱法   
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      • Vol. 16, Issue 2, Pages: 110-112(2010)
        摘要：Objective: To investigate the effect of Cyclovirobuxine D(CVB-D) on action potentials in isolated guinea pig ventricular myocytes. Methods: The single myocytes were enzymatically obtained from the guinea pig hearts and the action potentials were examined by whole-cell patchclamp technique. Results: 4×10-4 g/L CVB-D decreased action potential amptitude(APA)from 113.3±6.3 mV to 110.2±6.4 mV(n=6, P<0.01), prolonged action potential duration of repolarization 50%(APD50)from 616.3±68.0 ms to 657.4±79.5 ms(n=6, P<0.01),prolonged action potential duration of repolarization 90%(APD90)from 633.5±71.2 ms to 669.2±74.4 ms(n=6, P<0.01), Prolonged action potential duration of repolarization 50% to repolarization 90%(APD50-90)from 15.9±6.0 ms to 20.1±5.2 ms(n=6, P<0.01),and decreased decay slope of phase 3 (V3) from -2.6±0.8 V/s to -2.4±0.7 V/s(n=6, P<0.01).Compared with the baseline,4×10-3 g/L CVB-D makes the APD50-90 pass to increase trend gradually,but the variety trend of the V3 decrease gradually, other index sign varieties are consistent. Conclusion: CVB-D decreased APA and V3, and prolonged APD50,APD90,APD50-90,which may be its mechanism of positive inotropic effect and antiarrhythmic effect.  
          
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      • Vol. 16, Issue 2, Pages: 113-115(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.027
        摘要：目的:研究中药茜草中遗传毒性成分Lucidin及其苷Lucidin-3-O-primeveroside（EKU-4）在动物体内的代谢,对茜草的用药安全性进行初步考察。方法:采用反相高效液相法测定茜草70%乙醇提取物以及ig该提取物一定时间的动物血浆样品。结果:茜草提取物中均能够检测到Lucidin、EKU-4及大叶茜草素;茜草70%乙醇提取物,经ig给药后,血浆样品中仅能检测到Lucidin及大叶茜草素,并且给药15 min,Lucidin的血药浓度达到最高,给药后30 min,大叶茜草素的血药浓度达到最高。结论:由于ig给药前后血浆中未检测到EKU-4,而Lucidin成分有增加的趋势,因此推断中药茜草中EUK-4成分可能分解为Lucidin的形式参与动物体内代谢。  
        关键词：茜草;遗传毒性;Lucidin;Lucidin-3-O-primeveroside;大叶茜草素   
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      • Vol. 16, Issue 2, Pages: 113-115(2010)
        摘要：Objective: To observe the effect of Da-Fang-Feng-Tang(DFFT) in treating of Rheumatoid Arthritis(RA). Methods : The animal model of type Ⅱ collagen induced arthritis was used. Granules of the DFFT extract were administered by gastric gavage, studying in genetics, immunology, physiology and clinical. Results : The results indicated a higher onset rate, a severer redness and swelling for arthritic limb and a longer time of clinic manifestation in contract group than treated group, and the IgG level in sera was significantly increased compared contract group to treated group(P<0.05). The gene express of IL-1, TNF-α and IFN-γ were higher in contract group. Conclusion : DFFT express obviously suppressive function on model of type Ⅱ collagen induced arthritis in NIH mouse, and support the belief that DFFT is effective in treating human RA.  
          
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      • LIANG Wei,CAI Ying,ZHANG Li-ling,TANG Yong-ming,GONG Ling,JIN Lin,JIANG Ke-chun
        Vol. 16, Issue 2, Pages: 116-119(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.004
        摘要：Objective:To investigate the effect of Qingrehuayuziyin prescription on lymphocyte apoptosis and expression of Fas and Bcl-2 in peripheral blood in the patients with systemic lupus erythematosus（SLE）.Methods: Eighty patients with SLE were selected and divided randomly into two groups,40 in the therapy group and 40 in control group respectively.Patients were got the treatment of Qingrehuayuziyin prescription combined hormone as therapy group and got hormone treating only as control group.After treating three months,the patients lymphocyte were collected and the rate of apoptosis were detected by flow cytometer.Meanwhile,the expression of Fas and Bcl-2 were analyzed by RT-PCR.Results: After 3 months treating,the rate of lymphocyte apoptosis was decreased significantly in therapy group compared with that in control group and with that in pre-treating（P<0.01）.The expression of Fas and Bcl-2,compared with that in control group,were also decreased significantly（P<0.01）.Conclusion: Qingrehuayuziyin prescription is useful for treating SLE by suppressing lymphocyte apoptosis,decreasing the level of Fas and Bcl-2 in peripheral blood and further modifying the immune status.  
        关键词：Systemic lupus erythematosus;Qingrehuayuziyin prescription;apoptosis;Fas;Bcl-2   
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      • LIANG Wei,CAI Ying,ZHANG Li-ling,TANG Yong-ming,GONG Ling,JIN Lin,JIANG Ke-chun
        Vol. 16, Issue 2, Pages: 116-119(2010)
        摘要：Objective: To investigate the effect of Qingrehuayuziyin prescription on lymphocyte apoptosis and expression of Fas and Bcl-2 in peripheral blood in the patients with systemic lupus erythematosus (SLE). Methods : Eighty patients with SLE were selected and divided randomly into two groups, 40 in the therapy group and 40 in control group respectively. Patients were got the treatment of Qingrehuayuziyin prescription combined hormone as therapy group and got hormone treating only as control group. After treating three months, the patients lymphocyte were collected and the rate of apoptosis were detected by flow cytometer. Meanwhile, the expression of Fas and Bcl-2 were analyzed by RT-PCR. Results: After 3 months treating, the rate of lymphocyte apoptosis was decreased significantly in therapy group compared with that in control group and with that in pre-treating(P<0.01). The expression of Fas and Bcl-2, compared with that in control group, were also decreased significantly (P<0.01). Conclusion : Qingrehuayuziyin prescription is useful for treating SLE by suppressing lymphocyte apoptosis ,decreasing the level of Fas and Bcl-2 in peripheral blood and further modifying the immune status.  
        关键词：Systemic lupus erythematosus;Qingrehuayuziyin prescription;apoptosis;Fas;Bcl-2   
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      • Vol. 16, Issue 2, Pages: 119-121(2010)
        摘要：Objective: To study the pharmacokinetics of curcumin microcystin in rats. Methods: The microcystin was orally administrated. After carotid artery cannulation, blood samples were collected after administration of 0.08, 0.25, 0.5, 0.75, 1, 2, 3, 4, 5.5 h, and tested by HPLC. Results: The various doses of curcumin concentration-time curve is bimodal; Cmax1 and Cmax2 of each dose group are as follows: high-dose(0.90, 1.20 mg/L), medium dose(0.43, 0.46 mg/L), low dose(3.16, 1.31 mg/L); AUC are 3.79, 2.60, 5.92 mg·h/L respectively. Cmax and AUC of low dose are greater than that of the high and medium doses. Conclusion: The Pharmacokinetics of curcumin were nonlinear process in rat, there may be the phenomenon of enterohepatic circulation.  
          
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      • Vol. 16, Issue 2, Pages: 119-121(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.002
        摘要：目的:观察足底反射区按摩对老年女性的睡眠和精神压力的影响。方法:将40例老年女性对象分为治疗组和对照组,治疗前后测量睡眠质量和精神压力的变化。结果:治疗组睡眠改善优于对照组,而精神压力两组有所降低,但无明显差异。结论:足底反射按摩对居家女性的睡眠质量改善明显。  
        关键词：足底反射按摩;高龄居家女性;压力;理想睡眠   
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      • Vol. 16, Issue 2, Pages: 121-123(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.045
        摘要：目的:探讨育宫颗粒冲剂合用克罗米芬治疗多囊卵巢综合征（PCOS）不孕的临床疗效。方法:按随机分组法设育宫颗粒合用克罗米芬116例为治疗组;单独使用克罗米芬为对照组,观察治疗前、治疗6个周期后和停药4个周期,患者性腺激素的变化、卵泡发育变化、排卵率和妊娠情况。结果:两组总疗效比较,对照组为76%,观察组为92.2%,具有显著性差异（P<0.01）。结论:育宫颗粒合用克罗米芬治疗多卵巢综合征不孕,较单独使用克罗米芬能显著提高卵泡发育质量以及排卵率和受孕率。  
        关键词：多囊卵巢综合征;育宫颗粒;克罗米芬   
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      • Vol. 16, Issue 2, Pages: 124-125(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.003
        摘要：目的:观察茵陈五苓散对妊娠肝内胆汁淤积症（ICP）患者妊娠结局的影响。方法:68例ICP患者随机分为对照组和治疗组。对照组34例给予熊去氧胆酸片100 mg,口服,3次/d,连用20 d;维生素C 2 g,维生素B6200 mg,三磷酸腺苷40mg,辅酶A 100U,加入10%的葡萄糖液500 mL静脉滴注,1次/d治疗。治疗组34例在对照组基础上加用茵陈五苓散6 g,口服,每日3次治疗,两组疗程均为3周。比较两组治疗前后血清甘胆酸（CG）、总胆红素（TB）、血清谷丙转氨酶（ALT）和谷草转氨酶（AST）的变化,观察两组围产儿情况和妊娠结局。结果:两组治疗前后CG、ALT和AST的差值比较,差异有显著性（P<0.05）;治疗组新生儿窒息及羊水粪染率低于对照组（P<0.05）;对照组妊娠终止时间明显低于治疗组（P<0.01）,剖宫产率高于治疗组（P<0.05）。结论:茵陈五苓散可降低CG、TB、ALT和AST,改善ICP患者妊娠结局。  
        关键词：胆汁淤积;妊娠并发症;茵陈五苓散;妊娠结局   
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      • HE Pei-wen,ZHAO Hai-yu,DU Gang,WANG Yi-tao
        Vol. 16, Issue 2, Pages: 126-134(2010)
        摘要：Objective: In order to evaluate the safety of Chinese medicine comprehensively, the development of pesticide residues determination in Chinese medicine by gas chromatography (GC) was summarized in this paper. Methods : Through analytic comparison, the developments of sample pretreatments were summarized systematically, including solid-phase extraction (SPE), gel permeation chromatography (GPC), supercritical fluid extraction (SFE), accelerated solvent extraction (ACE), matrix solid phase dispersion (MSPD), solid-phase micro-extration (SPME) and headspace analysis (HS). In addition, the comparison of gas chromatography detectors such as mass spectrometry (MS), electron-capture detector (ECD), flame photometric detector (FPD) and nitrogen–phosphorus detector (NPD) were also discussed. Results: Gas chromatography is a mature technology to accomplish pesticide residues determination in Chinese medicine. Conclusion: Gas chromatography and relevant new techniques are wildly used in safety evaluation of Chinese medicine and its preparations, which will be benefit for the international development and import of Chinese medicine.  
        关键词：Chinese medicine;pesticide residues;gas chromatography;sample preparation   
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      • HE Pei-wen,ZHAO Hai-yu,DU Gang,WANG Yi-tao~
        Vol. 16, Issue 2, Pages: 126-134(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.020
        摘要：Objective:In order to evaluate the safety of Chinese medicine comprehensively,the development of pesticide residues determination in Chinese medicine by gas chromatography（GC） was summarized in this paper.Methods :Through analytic comparison,the developments of sample pretreatments were summarized systematically,including solid-phase extraction（SPE）,gel permeation chromatography（GPC）,supercritical fluid extraction（SFE）,accelerated solvent extraction（ACE）,matrix solid phase dispersion（MSPD）,solid-phase micro-extration（SPME） and headspace analysis（HS）.In addition,the comparison of gas chromatography detectors such as mass spectrometry（MS）,electron-capture detector（ECD）,flame photometric detector（FPD） and nitrogen–phosphorus detector（NPD） were also discussed.Results: Gas chromatography is a mature technology to accomplish pesticide residues determination in Chinese medicine.Conclusion: Gas chromatography and relevant new techniques are wildly used in safety evaluation of Chinese medicine and its preparations,which will be benefit for the international development and import of Chinese medicine.  
        关键词：Chinese medicine;pesticide residues;gas chromatography;sample preparation   
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      • MA Li~1,JIN Cheng~3,LI Zu-lun~2,XIAO Xiao-he~
        Vol. 16, Issue 2, Pages: 134-136(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.019
        摘要：Based on the current foundation of Radix Isatidis,possibility of quality evaluation of Radix Isatidis by bioassay is discussed.That is to evaluate quality by controlling the overall effect of Radix Isatidis.A new idea and method are provided for quality control of Radix Isatidis.  
        关键词：bioassay;Radix Isatidis;quality evaluation   
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      • MA Li,JIN Cheng,LI Zu-lun,XIAO Xiao-he
        Vol. 16, Issue 2, Pages: 134-136(2010)
        摘要：Based on the current foundation of Radix Isatidis, possibility of quality evaluation of Radix Isatidis by bioassay is discussed. That is to evaluate quality by controlling the overall effect of Radix Isatidis. A new idea and method are provided for quality control of Radix Isatidis.  
        关键词：bioassay;Radix Isatidis;quality evaluation   
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      • CHENG Ming
        Vol. 16, Issue 2, Pages: 137-138(2010) DOI: 10.13422/j.cnki.syfjx.2010.02.048
        摘要：This article discusses the confused situations when the traditional Chinese medicine material names are translated from Chinese into English,the wrong applications of the original Latin names,the inaccurate expressions of English words,the unscientific translations of Pinyin names.  
        关键词：latin name;traditional chinese medicine material name;english name;pinyin;translation   
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      • CHENG Ming
        Vol. 16, Issue 2, Pages: 137-139(2010)
        摘要：This article discusses the confused situations when the traditional Chinese medicine material names are translated from Chinese into English, the wrong applications of the original Latin names, the inaccurate expressions of English words, the unscientific translations of Pinyin names.  
        关键词：latin name;traditional chinese medicine material name;english name;pinyin;translation   
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      • Vol. 16, Issue 2, Pages: 143(2010)
          
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