最新刊期
卷 20 ， 期 20 ， 2014
- 摘要：Objective: To optimize β-cyclodextrin(β-CD) microcapsule extraction process of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma. Method: β-CD and sodium sulfate were employed as extraction agents in aqueous two-phase system, with composite score of volatile oil yield, quality of methyl eugenol and phthalides (in the content of ligustilide) as index, L9 (34) orthogonal test was adopted to optimize extraction and inclusion technology of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by β-CD microcapsule, the concentration of β-CD and sodium sulfate in extract, extraction methods and temperature were selected as main influence factors.Taking O-xylene and dibutyl phthalate as internal standards, components of volatile oil was analyzed by GC-MS. Result: Optimal extraction process conditions were as follows:soaked 24 h with 3 times the amount of 40% ethanol, heated to temperature of 60 ℃ and stirred for 60 min, then vacuum suction filtrated, β-CD and sodium sulfate were added to filtrate to the concentration of 300 g·L-1 and of 50 g·L-1, respectively;stirred and extracted for 30 min at 50 ℃.Under these conditions, yield of volatile oil was 86.54%, contents of methyl eugenol and phthalides (in the content of ligustilide) were 75.88 mg and 353.91 mg. Conclusion: Technologies for extraction and inclusion of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma are feasible and suitable for industrial production of volatile oil preparations for β-CD inclusion.关键词：Chuanxiong Rhizoma;Asari Radix et Rhizoma;β-cyclodextrin;methyl eugenol;microcapsule aqueous ewo-phase system;ligustilide;internal standard
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发布时间：2024-01-04 - 摘要：Objective: To prepare temperature sensitive type of Qingkailing ophthalmic in situ gels and evaluate its quality. Method: Gels matrix was optimized by single factor tests, Qingkailing ophthalmic in situ gels was prepared by the 2010 edition of 〈Chinese Pharmacopoeia〉, its quality was measured by factors of osmotic pressure, eye retention capacity, viscosity and in vitro release. Result: Taking mixture of 16% poloxamer 407 and 0.1% sodlium hyaluronate as gel matrix, Qingkailing injections lost completely in 9.9 min, while Qingkailing ophthalmic in situ gels could stay for a longer time of 30.6 min. Conclusion: Qingkailing ophthalmic in situ gels has good formability, namely accords with requirement of in situ gels.Compared with injections, this product can stay a longer time in application site and significantly increase viscosity with a good sustained release characteristics.关键词：temperature sensitive type;Qingkailing ophthalmic in situ gels;poloxamer 407;sodium hyaluronate;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To analyze main ingredients of essential oil from Fructus Alpiniae Zerumbet (EOFAZ) against human umbilical vein endothelial cells (HUVECs) injury induced by lipopolysaccharide (LPS). Method: Four factors were selected from EOFAZ (α-pinene, β-pinene, 1, 8-eucalyptol, camphene) as research object, each factor was selected two levels, including contained or not contained, experiment was arranged according to L8(27) Orthogonal test table.MTT activities and NO detection were used as indexes and data were analyzed by SPSS software.In component analysis method, protective effects of ingredients with different concentrations were assayed by testing cell viability and NO contents in medium. Result: Protective extent of four factor was camphene>α-pinene > 1, 8-eucalyptol > β-pinene, there were significant differences among these ingredients.Single component analysis showed that pretreatment with ingredients at different concentrations could obviously enhance cell viabilites and NO contents, and effects had dose-dependent. Conclusion: EOFAZ has a protective effect on HUVECs injury induced by LPS, and camphene, α-pinene, 1, 8-eucalyptol, β-pinene are main active components.关键词：essential oil from Fructus Alpiniae Zerumbet;lipopolysaccharide;human umbilical vein endothelial cells;single component analysis;cell viability
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of limonoids in Citri Reticulatae Semen by macroporous resin. Method: Static adsorption and desorption tests were adopted to select optimal type from 16 kinds of macroporous resin with different manufacturers and polarities.Purification process was optimized by single factor tests. Result: HPD-722 macroporous resin was selected, optimal purification process was as following:the concentration of sample solution between 3.503 3-5.403 4 g·L-1, sampling speed of 1-2 BV·h-1, pH of sample solution 6, washed impurity by 1 BV of water and eluted by 6 BV of 80% ethanol with elution rate of 2 BV·h-1, eluent pH of 7;under these conditions, purity of limonoids was 82.82%, including limonin of 22.53% and nomilin of 45.90%. Conclusion: Limonoids can be purified by HPD-722 macroporous resin, this process can effectively improve the purity of limonoids.关键词：Citri Reticulatae Semen;limonoids;macroporous resin;nomilin
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发布时间：2024-01-04 - 摘要：Objective: To prepare vinblastine hydrophilic group-modified cationic liposomes and investigate their physicochemical properties. Method: Vinblastine hydrophilic group-modified cationic liposomes were prepared by pH gradient method.Morphology of liposomes was detected by transmission electron microscopy, particle size distribution and Zeta potential was investigated by laser scattering, degree of oxidation was measured by oxidation index method, sephadex G-50 was applied to separate free drug and liposomes in order to determine entrapment efficiency and leakage efficiency of liposomes. Result: Particle size of liposomes was (102.4±3.8)nm with a narrow polydispersity index of 0.18±0.01, bilayer of these liposomes was observed obviously with a Zeta potential of (27.4±0.58)mV.Oxidation index of liposomes was less than 0.2, entrapment efficiency of vinorelbine was 86.42%.Leakage rate was less than 8.0% prevented in refrigerator (<4 ℃) after nine months, while this index was 34.8% at room temperature. Conclusion: Vinblastine hydrophilic group-modified cationic liposomes have uniform particle size and fine-looking with high entrapment efficiency.Leakage rate of liposomes is close to storage temperature.关键词：vinblastine;modified by hydrophilic group;cationic liposomes;physicochemical properties
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发布时间：2024-01-04 - 摘要：Objective: Nasal mucosa toxicity of cremophor RH40 and labrafil M 1944 CS were investigated, in order to explore feasibility of their application in nasal drug delivery system. Method: Compared with span-80 and tween-80, safety of surfactant was evaluated by testing cilia movement of in situ toad palate model and hemolysis test of erythrocyte membrane in rabbits. Result: When concentrations of these four kinds of surfactants were 1%, their effect on cilia movement was in order of cremophor RH40 < labrafil M 1944 CS < tween-80 < span-80. By survey of toxic effects on cell membranes, cremophor RH40 had little cell membrane toxicity, tween-80 and span-80 had moderate cell membrane toxicity, but labrafil M 1944 CS had serious cell membrane toxicity.Dosage of cremophor RH40 that had no effect on integrity of cell membrane was 15 times the amount of tween-80 and span-80. Conclusion: Cremophor RH40 has little toxic effect on nasal mucosa under a certain range of concentration that can be applied in nasal drug delivery system.关键词：nasal drug delivery system;toxicity of nasal mucosa;labrafil M 1944 CS;cremophor RH40
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of vinegar processing on contents of three characteristic ingredients(cyperotundone, nootkatone and α-cyperone) in Cyperi Rhizoma. Method: HPLC was employed to determine contents of these three characteristic ingredients in Cyperi Rhizoma before and after vinegar processing. A Phenomenex C18 column(4.6 mm×250 mm, 5 μm) was adopted with mobile phase of methanol-water(68: 32), detection wavelength of 242 nm, column temperature at 30 ℃ and flow rate of 1.0 mL·min-1. Result: This established determination was stable and feasible, linear ranges of these three characteristic ingredients were 0.260-0.912, 0.028-0.098, 0.082-0.286 μg, their average recoveries were 98.34%, 97.87%, 99.12% with RSD of 1.48%, 2.03%, 1.37%, respectively.Contents of cyperotundone, nootkatone and α-cyperone in Cyperi Rhizoma from eleven different origins before vinegar processing were 1.52-4.80, 0.04-0.20, 0.70-1.99 mg·g-1, but after been processed, they were 1.55-4.96, 0.04-0.19, 0.61-1.97 mg·g-1, respectively. Conclusion: Contents of these three ingredients are very different in Cyperi Rhizoma from different origins.The content of cyperotundone increases, while contents of nootkatone and α-cyperone decrease after been processed by vinegar.This study can provide a reference for mechanism study of vinegar processing research on Cyperi Rhizoma.关键词：Cyperi Rhizoma;vinegar processing;cyperotundone;nootkatone;α-cyperone
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Wenyang Huoxue granules. Method: HPLC was adopted to determine the content of paeoniflorin with mobile phase of acetonitrile-0.1% phosphoric acid solution (14:86) and detection wavelength at 230 nm;HPLC-ELSD was employed to determine the content of ginsenoside Rg1, Re, Rb1with mobile phase of acetonitrile(A)-water(B) for gradient elution (0-35 min, 19%A;35-55 min, 19%-29%A;55-70 min, 29%A;70-100 min, 29%-40%A).With yield of dry extract and total contents of paeoniflorin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 as comprehensive evaluation index, orthogonal design was taken for investigating effects of the amount of water, extraction times and time on extraction technology. Result: Optimal extraction conditions were as followings:reflux extracted thrice with 8 times the amount of water for 1.5 hours each time;yield of dry extract was 39.64%, contents of ginsenoside Rg1, Re, Rb1 and paeoniflorin were 3.49, 2.79, 6.64, 218.60 mg. Conclusion: This optimized technology is reasonable, simple and suitable for industrial production of Wenyang Huoxue granules.关键词：Wenyang Huoxue granules;Ginsenoside Rg1;Ginsenoside Re;ginsenoside Rb1;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To investigate influencing factors of kneading method for preparing solid dispersions of water-insoluble drugs by dissolution test under condition of supersaturation.Stability and formulation of solid dispersions were also studied preliminarily. Method: Indomethacin was selected as model drug, solid dispersions were prepared by kneading method, impacts of carrier types, kneading solvent and time on dissolution effects were investigated by single factor tests.Indomethacin solid dispersions were characterized by powder X-ray diffraction;stability test was performed under condition of 40 ℃ and relative humidity of 75% in an environmental test chamber;solid dispersions was formulated in a tablet dosage form by direct compression method. Result: Indomethacin solid dispersions were prepared with poloxamer 188 as a carrier, ethanol-water(1:1) as kneading solvent and kneading time of 30 minutes, which performed well in dissolution test and the maximum solution concentration was 29 times that of indomethacin material;solid dispersions was stable within one month under these test condition;these compressed solid dispersion tablets were bright and clean, whose parameters fitted the standard of 2010 edition of 〈Chinese Pharmacopoeia〉. Conclusion: Dissolution of solid dispersions can be enhanced to a great extent by kneading method, they can easily compress to tablets by direct compression method.关键词：kneading method;solid dispersions;supersaturation;powder X-ray diffraction;powder direct compression method;indomethacin
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发布时间：2024-01-04 - 摘要：Objective: To investigate feasibility of dynamic optimization extraction method (DOEM) in extraction process of Gastrodiae Rhizoma. Method: Extraction amounts of gastrodin and parishin were regarded as indexes, based on single factor test, orthogonal design was adopted to optimize extraction process of Gastrodiae Rhizoma with solvent amount, extracting time and times as factors.Based on orthogonal design, DOEM was employed to optimize extraction condition, then extraction effects of these two processes were compared by concents of marker components, extract yield and similarity evaluation of fingerprint. Result: Optimum extraction process of orthogonal design was as follows:extracting for thrice with 8-folds of 50% ethanol, 2 hours each time;contents of gastrodin and parishin were 0.50 mg·g-1 and 1.44 mg·g-1.Extraction technology of DOEM was as follows:extracting for 3 times with 8-folds of 50% ethanol, extracting time of 80, 40, 20 minutes;contents of gastrodin and parishin were 0.43 mg·g-1 and 1.37 mg·g-1.Relative deviations of marker components concents and total solids yield in these two processes were less than 2% with a 100% of similarity in fingerprint, indicating basic coincidence between extracts by these two processes.DOEM could save 67% of extraction time and 62% of power consumption. Conclusion: Extracts of these two methods are no difference, DOEM can significantly reduce extraction time and production costs, which means it is worth spreading in traditional Chinese medicine industry.关键词：dynamic optimization extraction method;Gastrodiae Rhizoma;gastrodin;parishin;fingerprint similarity
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发布时间：2024-01-04 - 摘要：Objective: To optimize molding technology of Qigui dropping pills. Method: Taking dissolution time of dropping pills, pill weight coefficient of variation, appearance score(roundness, hardness, color) as a comprehensive assessment index, based on single factor tests, orthogonal test was adopted to optimize molding technology by taking proportion of drug extract-matrix, dropping distance, material temperature and dropping speed as factors. Result: The best molding technology of Qigui dropping pills was as following:with polyethylene glycol 4000 as matrix, proportion of drug extract-matrix 1:2, material temperature 85 ℃, dropping speed of 45 d·min-1, dropping distance of 9 cm, with atolin as coolant, cooling column length of 110 cm, coolant temperature at 12 ℃;dissolution time was 5.02 min, pill weight difference was less than 10%. Conclusion: This optimized molding technology is stable and feasible.These prepared Qigui dropping pills have high molding rate to meet quality requirements of dropping pills.关键词：Qigui dropping pills;molding technology;liquid paraffin;polyethylene glycol;pill weight difference
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Fufang Guangjinqiancao granules. Method: With composite score of contents of schaftoside and total flavonoids, yield of dry extract as index, orthogonal test was adopted to optimize water extraction technology by taking the amount of water, extracting time and temperature as factors;taking composite score of contents of total polysaccharides and total flavonoids, removal rate of impurity as index, alcohol precipitation process of water extract was optimized by orthogonal test.With composite score of contents of 23-acetyl alisol B and total flavonoids, yield of dry extract as index, alcohol extraction technology of this compound was optimized by orthogonal test.Contents of schaftoside and 23-acetyl alisol B were determined by UPLC, mobile phase were methanol-0.4% phosphoric acid solution(32:68) and acetonitrile-water(73:27), respectively;UV was employed to measure contents of total flavonoids and total polysaccharides. Result: Optimum water extraction conditions were as follows:extracted 3 times with 8-folds of water for 1.5 h each time;optimal alcohol precipitation process was:alcohol precipitation concentration 60%, initial density of water extract 1.0 g·mL-1, alcohol sedimentation time for 8 h;optimum alcohol extraction conditions were as follows:extracted 2 times with 8, 6-folds of 60% ethanol, extracting time of 1.0, 0.5 h, respectively. Conclusion: These optimized extraction processes are stable, feasible and suitable for industrial production of Fufang Guangjinqiancao granules.关键词：Fufang Guangjinqiancao granules;schaftoside;23-acetyl alisol B;total flavonoids;total polysaccharides;apigenin;rutin
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发布时间：2024-01-04 - 摘要：Objective: To optimize spray drying process of Ejiao powder. Method: Taking dry powder yield and moisture content as indexes, effects of outlet and inlet air temperature, relative density of glue, feed rate on spray drying process were investigated by single factor tests and orthogonal test. Result: Optimum process conditions was as following:relative density of 1.20 (60 ℃), feed rate of 40 mL·min-1, inlet air temperature 165 ℃, outlet air temperature of 65 ℃;dry powder yield and moisture content were 81.6% and 4.26%, respectively. Conclusion: This optimized process is stable and feasible, itcan provide experimental basis of raw material drying method selection for granules, tablets, capsules and convenient dosage forms, and it provides ideas for drying methods of animal glue oftraditional Chinese medicine.关键词：spray drying process;Ejiao powder;dry powder yield;moisture content
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination equilibrium solubilities of total saponins extract from Aralia chinensis in different mediums. Method: Taking araloside A as index, an HPLC method was performed and adopted to detect the concentration of araloside A in water, 0.1 mol·L-1 HCl and different phosphate buffer with pH of 2, 4, 6, 7, 8, 10 at 37 ℃, mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution (0-18 min, 35%-43%A;18-19 min, 43%A;19-25 min, 43%-35%A), detection wavelength was set at 205 nm. Result: Equilibrium solubilities of araloside A in different mediums were 1.780, 0.241, 0.420, 0.472, 1.196, 1.746, 0.180, 0.242 g·L-1. Conclusion: Total saponins extract from A. chinensis dissolves in water, its equilibrium solubility is influenced greatly by pH values.关键词：total saponins from Aralia chinensis;araloside A;equilibrium solubility;phosphate buffer
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish an HPLC-DAD method for the determination of 2-methy-3-methoxyanthraquinone, 2, 3-dimethoxy-6-methyanthraquinone in Hedyotidis Herba. Method: The assay was performed on a ZORBAX Extend-C18 column(4.6 mm×250 mm, 5 μm) eluted with acetonitrile -0.5% phosphate(48:52).The detection wavelength was 265 nm, the flow rate was 1.0 mL·min-1, and the column temperature was set at 25 ℃. Result: The calibration curves were linear in the ranges of 0.998-9.98 μg(r=0.999 6)for anthraquinone I and 0.675-6.75 μg(r=0.999 9)for anthraquinone II. The average recoveries of anthraquinone I and anthraquinone II were 99.87%(RSD 1.13%)and 99.51%(RSD 0.69%), respectively. Conclusion: The method is simple and accurate for the quality control of 2-methy-3-methoxyanthraquinone and 2, 3-dimethoxy-6-methyanthraquinone in Hedyotidis Herba.关键词：Hedyotidis Herba;2-methy-3-methoxyanthraquinone;2, 3-dimethoxy-6-methyanthraquinone;HPLC-DAD
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish a method for simultaneous assay of eight kinds of active ingredients in Lonicerae Japonicae Flos (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, cynaroside, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C) by quantitative analysis of multi-components by single marker (QAMS) and external standard method (ESM), and determine the application value of QAMS in the quality control of Lonicerae Japonicae Flos. Method: The chromatographic separation was carried out on a ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm) column with gradient elution of acetonitrile and 0.1% phosphoric acid at a flow rate of 1 1 mL·min-1. The detection wave-length was 325 nm and 350 nm.A characteristic spectrum was used for identification of eight components. By using chlorogenic acid as reference, the relative corrective factors (RCF) of eight components were calculated.The method was evaluated by comparison of the quantitative results through external standard method and QAMS method. Result: The results from QAMS method were not significantly different from those from external standard method. Conclusion: The method is of high sensitivity and nice reproducibility, and it could be used to control the quality of Lonicerae Japonicae Flos with QAMS.关键词：Lonicerae Japonicae Flos;quantitative analysis of multi-components by single marker;relative correction factor;content determination
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发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to provide basis for grading of Coptis Rhizoma pieces through with the inner quality and appearance features of Coptis Rhizoma pieces. Method: Eighteen batches of Coptis Rhizoma pieces were selected as analysis object, subjected to measurement of the size (length, width, thickness and weight), color (external color, internal color), and content (table of berberine, hydrastis, martin, berberine). Then SPSS cluster analysis was conducted based on the measurement results. Result: Qualified ones were divided into two grades:superior class and ordinary class. Conclusion: Inner quality evaluation and appearance feature quantification can be used to classify Coptis Rhizoma pieces through the mathematical statistics analysis.关键词：Two-step mothod;Coptis Rhizoma pieces;appearance property features;inner quality evaluation quantification;classification
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发布时间：2024-01-04 - 摘要：Objective: This article aimed to study the chemical components of essential oils from Chloranthus henryi and their antioxidant activity. Method: The essential oil was extracted by steam distillation, and its constituents were identified by GC-MS.The antioxidant activity of the essential oil was evaluated on its ability of total reducing and scavenging of 1-diphenyl-2-picrylhydrazy (DPPH) free radical by spectrophotometry. Result: Twenty-one compounds, accounting for 82.30% of the essential oil, were identified. The ability of scavenging DPPH free radical of the essential oils was weak, while the total reducing ability was strong. Conclusion: The main chemical constituents of the essential oil from C. henryi were bornyl acetate (45.43%), 3-methylene-2-norbornanone(12.36%) and camphene (8.74%). And the essential oil showed some antioxidant activity.关键词：Chloranthus henryi;volatile oil;chemical constituents;GC-MS;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: This study was aimed to establish disperse liquid-liquid microextraction coupled with high performance liquid chromatography(DLLME-HPLC) method for determination of five pyrethroid pesticides (flucythrinate, fenvalerate, lambda-cyhalothrin, permethrin, bifenthrin) in Lonicerae Janponicae Flos. Method: The five pyrethroid pesticides in Lonicerae Janponicae Flos were extracted by trichlormethane, with acetonitrile as dispersant, then analysed by HPLC.Some important parameters that influenced the extraction efficiency were optimized. The five pesticides were separated by an Agilent ZORBAX XDB-C18(4.6 mm×250 mm, 5 μm) column in the wavelength at 230 nm. The mobile phase was acetonitrile-H2O with gradient elution and the flow rate was 1.0 mL·min-1. Result: Flucythrinate, fenvalerate, lambda-cyhalothrin, permethrin and bifenthrin showed good linear relation within range of 0.05-5.0 mg·L-1 (r>0.999 0). The enrichment factors ranged from 18.6 to 29.7.The average recovery of high, medium and low concentration ranged from 72.1% to 110.4%. Conclusion: The method is accurate and sensitive, simple and fast, and can meet the requirement of pyrethroid pesticedes residue analysis in Lonicerae Janponicae Flos.关键词：Lonicerae Janponicae Flos;pyrethroid pesticedes;disperse liquid-liquid microextraction;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The puepose of this study was to determine the protein content in the root and stalk of Stellera chamaejasma , and to analyze the amino acid composition. Method: The protein content was determined by spectrophotometry with colored by coomassie brilliant blue G-250;the amino acid composition were analyzed by automatic amino acid analyzer. Result: The protein content in the root and stalk of S. chamaejasma were 12.26 mg·g-1 and 3.93 mg·g-1, respectively. There were 14 kinds of amino acids both in the root and stalk of S. chamaejasma, with 13 common amino acids. Of the 14 amino acids, the root contained met but in the absence of ser, and the stalk lacked met, while contained ser. The root contained 6 kinds of essential amino acids for human body, while the stalk contained 5.The amino acid content in the root was in a range of 0.116 4-39.561 3 mg·g-1, while that in the stalk was in a range of 0.026 1-0.469 1 mg·g-1. Conclusion: The contents of protein and amino acid in the root were higher than those in the stalk of S. chamaejasma. So the root has higher medical value than stalk in terms of protein and amino acid content.关键词：Stellera chamaejasma;bovine serum albumin;protein;amino acid
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发布时间：2024-01-04 - 摘要：Objective: The aim of this stydy was to establish a method to determine paeoniflorin, ferulic acid and hesperidin in Ruanjian Xiaoying granules. Method: The chromatographic separation was performed on Agilent TC-C18(4.6 mm×250 mm, 5 μm) column. The mobile phase was acetonitrile-0.2% acetic acid(18:82). The flow rate was 1.0 mL·min-1and column temperature was maintained at 25 ℃. The detection wavelength was set at 230 nm within 0-12 min, 325 nm within 12-20 min, 283 nm within 20-40 min. Result: The three compounds were separated well. The linear ranges of paeoniflorin, ferulic acid and hesperidin were 0.061 7-1.541 7 mg(r=0.999 9, average recovery was 97.25%, RSD 1.45%), 0.005 9-0.146 7 mg(r=0.999 7, average recovery was 98.66%, RSD 1.45%) and 0.136 7-3.416 7 mg(r=0.999 6, average recovery was 97.55%, RSD 1.92%). Conclusion: The method is rapid, simple and accurate, and can be used for quality control of Ruanjian Xiaoying granules.关键词：Ruanjian Xiaoying granules;paeoniflorin;ferulic acid;hesperidin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous assay of 3 kinds of monoterpene paeoniflorin class and paeonol in Moutan Cortex by quantitative analysis of multi-components by single marker(QAMS). Method: A HPLC method was developed as QAMS to determine paeonol, paeoniflorin, oxidation of paeoniflorin and benzoyl paeoniflorin. in Moutan Cortex.Using paeonol as the internal reference substance, the relative correction factor(RCF) of the three components was determined by HPLC with good reproducibility.The contents of the four components were determined by QAMS and external standard method. Result: No siginificant differences between the quantitative results of QAMS method and external standard method were observe. Conclusion: It is accurate and feasible to control the quality of Moutan Cortex with QAMS of paeoniflorin, oxidation of paeoniflorin, benzoyl paeoniflorin.关键词：quantitative analysis of multi-components by single marker;Moutan Cortex;relative correction factor;paeonol;monoterpene paeoniflorin ingredient
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发布时间：2024-01-04 - 摘要：Objective: The self contrast and correction factor method was established to determine the content of the related substances in lisinopril and hydrochlorothiazide tablets. Method: Chromatography was carried out by reversed-phase technique to a Ultimate XB-C18 (4.6 mm×250 mm, 5 μm) with a mobile phase composed of 0.05 mol·L-1 phosphate buffer (pH 7.3±0.1) -acetonitrile (85:15) pumped at a flow-rate of 1.0 mL·min-1. The detection was carried out at a wavelength of 210 nm and a column temperature of 30 ℃. The injection volume was 10 μL. The slope of linear equation was used to determine the correction factor of lisinopril related compound A and benzothiadiazine related compound A. Result: The relative retention times of lisinopril related compound A and benzothiadiazine related compound A were 1.88 and 0.69, respectively. The correction factors of lisinopril related compound A and benzothiadiazine related compound A were 1.16 and 0.87, respectively. The contentsof benzothiadiazine related compound A from three batches of samples were 0.671%, 0.673% and 0.660%. Other impurities werent detected. Conclusion: The method was simple, efficient and accurate for analyzing the related substances in lisinopril and hydrochlorothiazide tablets.关键词：lisinopril and hydrochlorothiazide tablets;the related substances;the correction factor;the self contrast
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发布时间：2024-01-04 - 摘要：Objective: To establish one quick method for the determination of five carbamate pesticides including carbaryl, methomyl, aldicarb, suncide and carbofuran in Rosa Roxburghii Fructus by using SPE and HPLC-MS-MS. Method: The sample was cheated by high speed homogenizer, extracted by acetonitrile, cleaned by carbon/NH2 cartridge, separated by gradient elution, the chromatographic condition were column: Thermo Hypersil GOLD columns(2.1 mm ×100 mm, 1.9 μm), temperature 20 ℃, speed 300 μL·min-1, inject 10 μL.Five pesticides were detected with positive-ion electro-spray ionization mass spectrometry in MRM model. The Mass spectrometry condition were spray volage 3.0 kv, sheath gas 40 psi, collision gas pressure 1.5 mTorr, capillary temperature 350 ℃, vaporizer temperature 250 ℃. Result: The calibration curve was liner in the range of 0.5-100 μg ·L-1(r>0.999), the limits of detection was 0.2 μg·kg; the recoveries were in the range from 77.9% to 107.2% at low, middle and high levels, RSD were 4.5%-13.2%. Conclision: The method is rapid, sensitive and suitable for analyzing carbamate pesticides residues in Rosa Roxburghii Fructus .关键词：liquid chromatography-tandem mass spectrometry;carbamate pesticides;Rosa Roxburghii Fructus
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical components of essential oils from Kadsurae Oblongifoliae Radix and investigate their effect on proliferation of breast cancer cell line MCF-7. Method: The essential oil was extracted by steam distillation, and analyzed by GC-MS. Comparing with the data base NIST08 and WILEY275, and combing with Kováts retention index to identify the essential oil constituents. MTT assays were used to measure the inhibitory effect on the human breast cancer cell MCF-7. Result: Fifty compounds, account for 98.421% of the essential oils, were identified.The volatile oil has inhibitory effect on the growth of MCF-7 cells, and the effect is concentration dependant, IC50 377.61 mg·L-1. Conclusion: The main chemical constituents of the essential oils from Kadsurae Oblongifoliae Radix are camphene (17.770%), borneol (12.080%), bicyclo heptan-2-ol, 1, 7, 7-trimethyl-2-acetate (8.239%) and (+)-delta-cadinene (7.315%). And the essential oils of Kadsurae Oblongifoliae Radix can inhibit proliferation of MCF-7 cells, which shows concentration dependence.关键词：Kadsurae Oblongifoliae Radix;essential oil;GC-MS;retention index;MCF-7;cell proliferation
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发布时间：2024-01-04 - 摘要：Objective: To study fat-soluble components in leaves of Malus halliana Koehne. Method: The fat-soluble components of in leaves of M. halliana were analyzed by GC-MS for the first time. Result: Eighty-eight compounds were identified from the leaves of M. halliana, accounting for 80.79% of the total constituents. Conclusion: Esters (25.66%), acids (19.01%), alkanes (14.68%) and alcohols (11.33%) were the main compound types in leaves of M. halliana.Its main components were palmitic acid (14.49%), phytol (10.72%), ethyl palmitate (5.32%), linoleic acid (4.65%), 2-methyl tetrahydrofuran (4.22%), methyl palmitate (4.07%) and hexane (4.03%).关键词：Malus halliana Koehne;fat-soluble components;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-hepatitis B virus (HBV) constituents of Pogonatherum crinitum. Method: The compounds were isolated and purified by recrystallization and column chromatography over silica gel, polyamide and Sephadex LH-20.Their structures were elucidated on the basis of physicochemical properties andspectral analysis. Result: Six compounds were identified as luteolin-6-C-β-D-chinovoside (1), vitexin rhamnoside (2), apigenin-6-C-β-D-glucopyrano-4'-O-α-L-rhamnoside (3), hyperin (4), quercetin-3-O-methyl ether (5), D-mannitol (6). Conclusion: All compounds were isolated from the genus of Pogonatherum for the first time.关键词：Gramineous;Pogonatherum;Pogonatherum crinitum;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Zanthoxylum scandens. Method: The compounds were isolatedfrom the ethanol extract of Z. scandens by silica gel column chromatography etc. Their chemical structures were elucidated by spectral data. Result: Six compounds were isolatedas 6, 7-dimethoxy-coumarin(1), eudesmin(2), sesamin(3), N-benzoyl-L-phenylalaninol(4), 3β, 28-dibydroxylup-20(29) ene (betulin) (5), β-sitosterol(6). Conclusion: Compounds 1-5 were separated from this plant for the first time.关键词：Miao medicine;Zanthoxylum scandens;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the anti-inflammatory fraction of Caesalpinia decapetala. Method: The compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography and their structures were determined by chemical evidence and pectral analysis. Result: Nine compounds were obtained from C. decapetala and they were identified as (±) protosappanin B (1), scopoletin-7-O-β-D-glucopyranoside (2), 4-hydroxy-3-methoxypheny-1-O-β-D-glucopyranoside (3), (1'R, 3'S, 5'R, 8'S, 2Z, 4E)-dihydrophaseic acid-3'-O-β-D-glucopyranoside (4), (1'R, 3'S, 5'R, 8'S, 2E, 4E)-dihydrophaseic acid-3'-O-β-D-glucopyranoside (5), liquiritigenin (6), isoscopoletin (7), 7-hydroxy-3-(4'-hydroxybenzylidene)-chroman-4-one (8), 3-deoxysappanchalcone (9). Conclusion: Compounds 2-5, 7 were isolatedfrom thegenus C. for the firs time, and compounds 6, 8 were obtained from the C. decapetala for the first time.关键词：Fabaceae;Caesalpinia decapetala;chemical constituents;structure determination
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发布时间：2024-01-04 - 摘要：Objective: To study the non-alkaloid constituents of Buxus sinica. Method: The compounds from ethyl acetate and n-butanol fractionsof B. sinica were isolated byvarious chromatographic techniques including MCI gel, normal phase silica gel, RP-18 Silica geland Sephadex LH-20.The structures were identified by their physical-chemical properties and spectroscopic analysis. Result: Ten compounds were isolated and identified as 4', 5-dihydroxy-3, 3', 6, 7-tetramethoxyflavone(1), 4', 5-dihydroxy-3, 6, 7-trimethoxyflavone(2), 3', 4', 5-trihydroxy-3, 6, 7-timethoxyflavone(3), pinoresino(4), 3'-O-demethylepipinoresionl(5), 4-hydroxy-3-methoxycinnamic acid methyl ester(6), methyl-4-hydroxycinnamate(7), cleomiscosin A(8), syringin(9) and isolariciresinol-4-O-β-D -glucopyranoside(10). Conclusion: Compounds 1, 4-7, and 9-10 were isolated from this plant for the first time.关键词：Buxus sinica;non-alkaloid constituents;flavones;lignans
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Eucommiae Folium. Method: The compounds were isolated and purified byvarious column chromatography techniques, the chemical structureswere elucidated by physiochemical properties and spectral analysis. Result: Ten compounds were isolated and identified asarctiin(1), epipinoresinol(2), dihydroxydehydrodiconiferyl(3), 3, 4-dihydroxy-benzoic acid(4), D-ribono-1, 4-lactone(5), L-ribono-1, 4-lactone(6), uridine(7), β-D-glucopyranosyl- (1→3) -α-D-glucopyranosyl- (1→2) -β-D-fructofuranoside(8), eucommiol(9), 1-deoxyeucommiol(10). Conclusion: Compounds 1, 4-8 were isolated from this plantfor the first time.关键词：Eucommiae Folium;chemical constituents;eucommiol
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发布时间：2024-01-04 - 摘要：Objective: To study the transport behavior of aconitine in the Caco-2 cell monolayer model. Method: MTT method was employed to confirm the safe concentration of aconitine towards Caco-2 cell.Aconitine concentrations in the samples were analyzed by high performance liquid chromatography (HPLC).The apparent permeability(Papp)value and the total amount of transport were calculated. Bi-direction transport behavior of aconitine by incubating in different concentration and time were studied. Result: The transport amount of aconitine was positively correlated with the incubation time and drug concentration on the Caco-2 monolayer model. The Papp values in both apical to basal (AP-BL)and basal to apical (BL-AP) directions were stable with no significant differences in selected concentrations. The BL-AP flux of aconitine was more than 1.5-fold higher than AP-BL flux(Papp 1×10-5cm·s-1). Conclusion: The results suggested that the aconitineis well absorbed through the Caco-2 monolayer model by a passive transportation. Besides, efflux protein may be involved in the transport process.关键词：aconitine;Caco-2 monolayer model;absorption;effuxion
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Buyang Huanwu decoction (BYHWD) on learning and memory ability and amyloid precursor protein (APP) of Hippocampal in Alzheimer's disease mouse. Method: APP/PSI double transgenic mice were randomly divided into control group, model group, treatment group (Piracetam 0.62 g·kg-1), BYHWD high-dose group(37.06 g·kg-1), BYHWD medial-dose group(18.53 g·kg-1), BYHWD low-dose group(9.26 g·kg-1). The mice were killed after 28 days continuous administration.The Morris water maze was used to observe the learning and memory ability for the mouse. Biochemical methods were used to determine the content of APP protein in the hippocampal tissue. Result: Compared with the control group, model group latencies and swimming distances significantly prolonged(P<0.05), APP significantly increased(P<0.05);compared with the model group, BYHWD could significantly improve the learning and memory ability of model mouse(P<0.05). BYHWD could decrease the content of APP significantly in the hippocampal (P<0.05). Conclusion: BYHWD could improve the ability of learning and memory of model mouse. BYHWD plays a certain role in the treatment of Alzheimer's disease through inhibiting APP.关键词：Buyang Huanwu Decoction;Alzheimer's disease;learning and memory ability;amyloid precursor protein
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of different proportions of Puerariae Lobatae Radix(PLR) and Coptidis Rhizoma(CR) on fasting blood glucose(FBG) of different diabetic mice model, and serum triglyceride (TG)and total cholesterol(TC)levels of diabetic mice mode induced by streptozotocin (STZ), further to explore the best ratio of PLR and CR against diabetes. Method: Male SPF mice were randomly divided into blank control group, metformin group, PLR and CR 10:0, 8:3, 6:5, 3:8, 0:10 groups;the mice diabetes model weas induced by alloxan and STZ, the tail blood was collected to determine the levels of FBG after 72 hours, the die mice were randomly divided into control group, model group, metformin group, PLR-CR 10:0, 8:3, 6:5, 3:8, 0:10 groups.To observe impact of different proportions of PLR and CR on the FBG of normal mice and alloxan-induced diabetic mice, FBG, TC and TG levels were measured. Result: 10:0, 8:3, 6:5, 3:8, 0:10 all groups could significantly reduce the FBG in mice induced by alloxan or STZ, reduce the serum TG and TC levels in mice induced by STZ combined with high fat diet. The group with 3:8 of PLR to CR had more obvious effect than CR group. Conclusion: The single and combined administration of PLR and CR and have efficacy against diabetes, and could significantly improve diabetes-related biochemical marker. The combine administration is better than single one.关键词：Puerariae Lobatae Radix;Coptidis Rhizoma;combine compatibility of traditional Chinese medicine;fasting blood glucose
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Biejiajian pills (BP)on human umbilical vascular endothelial cell(HUVEC) proliferation and vascular endothelial growth factor(VEGF) expression in HepG2 and to explore the mechanism of BP to suppress angiogenesis of hepatocellular carcinoma(HCC, HepG2). Method: Twenty-four Wister rats were randomized equally into 3 groups for gavage of BP at 20-fold and 10-fold clinical doses and normal saline for 3 days. Blood samples were then collected from the rats, and the serum was separated and added in HepG2 cell cultures. HUVEC was cultured in conditioned medium (CM) supplemented with culture medium supernatant of HepG2 for 48 h. Then the proliferation of HUVEC was measured by MTT colorimetry. The effect of drug serum on HUVEC proliferation stimulated by CM was investigated by MTT colorimetry. After cultured in the medium containing drug serum for 48 h, the concentration of VEGF in HepG2 culture medium was assayed by ELISA and the expression of VEGF mRNA was investigated by qRT-PCR. Result: High and middle dose BP inhibited the proliferation of HUVEC in a concentration-and-time depending manner. High and middle dose BP decreased the secretion of VEGF as well as the expression of VEGF mRNA significantly, which is also correlated with the concentration of BP. Conclusion: BP can effectively inhibit the proliferation of HUVEC and lower the expression of VEGF. It may serve as a potential anti-angiogenesis agent in HCC.关键词：hepatocellular carcinoma;Biejiajian pills;vascular endothelial growth factor;human umbilical vascular endothelial cell
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Cistanche deserticola polysaccharides herb (CDPS) on learning and memory ability of D-galactose(D-gal) induced aging mice and possible mechanisms. Method: Ninety mice were randomly assigned into control group, model group, CDPS low, middle and high dose group (25, 50, 100 mg·kg-1), positive control group, 15 mice in each group. The model, CDPS and positive control groups were injected 150 mg·kg-1 D-gal subcutaneously to establish aging-mouse model, and the control group was given the same volume of saline water;meanwhile, the CDPS groups were administered orally corresponding concentrations of CDPS liquid and positive control group was given 10 mg·kg-1 piracetam, while the control and the model groups were given the same volume of distilled water.After six weeks of continuous administration, the water maze performance and step-down passive avoidance test were applied to determine learning and memory ability of mice. Moreover, superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in brain tissue of mice were detected by kits. Morphological changes of neurons in the hippocampus of brain tissue were observed with HE staining. Furthermore, the expression level of cAMP response element binding protein(CREB) was examined by immunohistochemistry. Result: In water maze performance, CDPS(25, 50, 100 mg·kg-1) significantly decreased latency and the first time arrived platform and increased travel times in D-gal-treated mice. In step-down passive avoidance test, the step down latencywas significantly prolonged and the number of errors was decreased in CDPS(25, 50, 100 mg·kg-1)groupcompared to those in the D-gal-control group. In addition, CDPS(25, 50, 100 mg·kg-1) significantly increased the activity of SOD and decreased the level of MDA in the brain tissues of D-gal-treated mice. At the same time, CDPS increased the number of neuron in hippocampal CA1 of mouse brain and ameliorated the pathological changes according to HE staining and CDPS(25, 50, 100 mg·kg-1) increased CREB expression levels in the hippocampus of mice comparing with those in D-gal-control group. Conclusion: CDPS canimprove the learning and memory ability of D-gal induced aging model mice, and the mechanism may be related to increasing CREB expression.关键词：Cistanche deserticola polysaccharides;aging;learning and memory;CREB
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effects of Zuozhu Daxi on rats with chronic atrophic gastritis (CAG)caused by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG). Method: CAG model was established by drinking 170 mg·L-1 MNNG for 8 weeks. Then the model rats were randomly divided into 5 groups, which was administered with distilled water(10 mL·kg-1·d-1), Moluodan(0.36 g·kg-1·d-1), Zuozhu Daxi (0.4, 0.2, 0.1 g·kg-1·d-1). At the end, the pathologic changes of gastric mucosa were observed, and malondialdehyde (MDA), superoxide dismutase(SOD), interleukin 12(IL-12), nitric oxide(NO)in serum were tested. Result: Compared with the control group, the IL-12 of model group were decreased significantly(P<0.01).Compared with the model group, IL-12 levels in large and middle doses of Zuozhu Daxi were increased significantly (P<0.05, P<0.01), the amount of NO and MDA was reduced significantly(P<0.05, P<0.01). SOD had no significant difference between groups. The stomach tissue sections stained by HE showed that the relief of pathological changes of gastric mucosa in the treatment groups were much better than model group. Conclusion: Zuozhu Daxi has therapeutic effect on rats with chronic atrophic gastritis caused by MNNG.关键词：Zuozhu Daxi;chronic atrophic gastritis;N-methyl-N'-nitro-N-nitrosoguanidine;malondialdehyde;interleukin 12
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of different polar fraction of Erzhi pill on acute liver injure of rats induced by alcohol and its mechanism. Method: Water decoction of Erzhi pill was extracted in order by different solvents. After drying, petroleum leve, acetoacetate and n-butanol fractions were obtained, and dissolved by water. Seventy male Wistar rats were randomly divided into seven groups. The model of alcoholic liver injury in rats was established by giving alcohol ig per day for 28 days. The serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alcohol dehydrogenase (ADH) and triglycerides (TG), serum endotoxin, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-18 (IL-18) were assayed. And hepatic superoxide dismutase(SOD), malondialdehyde(MDA) were measured respectively. Hepatic histopathology was observed by microscope. Result: Combined with the control group, serum levels of ALT, AST, ADH, TG, serum endotoxin, TNF-α, IFN-γ and IL-18 of the model group increased significantly (P<0.01), indicating that the model was established successfully;and the increasing levels of ALT, AST, ADH, TG, serum endotoxin, TNF-α, IFN-γ and IL-18 of all the treatment groups were inhibited (compared with model group, P<0.05 or P<0.01).Combined with the control group, hepatic levels of MDA and SOD in model group increased or decreased significantly (P<0.01), the increasing levels of MDA in the tiopronin group, petroleum leve and acetoacetate groups were inhibited (compared with model group, P<0.05 or P<0.01). The decreasing levels of SOD in the tiopronin group, acetoacetate, n-butanol and raffinate groups were inhibited (compared with model group, P<0.05 or P<0.01). The histopathological analysis suggested that all of the treatment groups could significantly ameliorate pathological changes of hepatocytes. Conclusion: Ethyl acetate fraction of Erzhi pill has the protective effect on liver injury of rats induced by alcohol, the mechanism might be related to endotoxin-CD14/TLR4.关键词：Erzhi Pill;alcohol;different polar fraction;liver injury
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Oviductus Ranae on the content of testosterone, substance metabolism and anti-fatigue ability of rats after exercise. Method: By using the model of high-intensity endurance training, fifty-five male Wistar rats were randomly divided into 5 groups, with 10 in each group. The rats which did not meet the requirement were removed:still in control group (C group), motion control group (M group), exercise +ig low-dose Oviductus Ranae group (OML group), exercise+ig middle-dose Oviductus Ranae group (OMM group), and exercise+ig high-dose Oviductus Ranae group (OMH group). Gavage was performed using professional device once a day. The rats in Oviductus Ranae groups were gavaged with 0.5, 1.0, 3.0 g·kg-1 with ig volume of 5 mL·kg-1. The rats in C and M groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: The body weights of the rats in M groups were lower than those in C group (P<0.05), and those in all doses of Oviductus Ranae groups were higher than in M group (P<0.05) which showed no differences between groups. Swimming time in all doses of Oviductus Ranae groups was longer than M group (P<0.01)with a dose-effect relationship, But there were no differences between C and M groups. Serum testosterone in M group was lower than C group (P<0.01), in the same time, serum testosterone was higher in all doses of Oviductus Ranae groups than M group [OML:(4.96±1.60) nmol·L-1;OMM:(5.19±1.60) nmol·L-1;OMH:(5.35±1.66) nmol·L-1, P<0.01]. The serum corticosterone levels in each group showed no significant differences. Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. Liver glycogen and muscle glycogen (P<0.01) in M group were lower than in C group, and in all doses of Oviductus Ranae groups were higher than in M group (liver glycogen, P<0.05;muscle glycogen, P<0.01). There were no differences in luteinizing hormone and follicle-stimulating hormone between groups. The serum urea nitrogen in M group was higher than C group (P<0.01), and in all doses of Oviductus Ranae groups was lower than the M group (P<0.01). Hemoglobin in M group was lower than C group (P<0.01), and in all doses of Oviductus Ranae groups were higher than in M group(P<0.01). Conclusion: Oviductus Ranae can alleviate the impact of high-intensity exercise on serum testosterone, and maintained it at normal physiological levels;it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.关键词：Oviductus Ranae;testosterone;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to ascertain the synergistic analgesic interaction and potential mechanism of sodium ferulate (SF) and ginsenoside Rg1 (Rg1). Method: Mice and rats were divided into SF low, medium and high dose groups, Rg1 low, medium and high dose groups, the combination low, medium and high dose groups and the control groups. After 5 days of intragastric administration, the hot plate test, the acetic acid writhing test and the formalin test were used to observe the analgesic effects of SF, Rg1 and the combination. The whole-cell patch-clamp was used to observe the effects of SF, Rg1 and on transient receptor potential vanilloid 1 (TRPV1). Result: Hot plate 55 ℃, 0.6% acetic acid solution 0.02 mL·g-1, 5% formaldehyde solution 0.1 mL caused obvious pain responses. The medium and high dose groups of SF, the high dose group of Rg1 and the low, medium and high dose groups of the combination showed good analgesic effects on the three models (P<0.05, P<0.01), and the effects of the combination were better than SF or Rg1 administrated alone (P<0.05, P<0.01).1 μmol·L-1 capsaicin (CAP) activated TRPV1 and induced specific inward current, SF, Rg1 and the combination inhibited the current and the inhibition of the combination was stronger than SF and Rg1 group (P<0.05, P<0.01). The 50% inhibition concentration (IC50) and 95% confidence interval of SF, Rg1 and the combination were 32.4 (27.5-38.7), 82.6 (71.0-98.4), 71.0 (20.2-41.0) mg·L-1respectively. The isobologram showed that the inhibition of SF and Rg1 on TRPV1 had a synergistic effect. Conclusion: The combination of SF and Rg1 had analgesic effect, the synergistic inhibition of may be one of its mechanisms.关键词：sodium ferulate;ginenoside Rg1;analgesic;transient receptor potential vanilloid 1
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Tangnaikang (TNK) on the smads signaling pathway of human renal tubular epithelial cells (HK-2) induced by transforming growth factor-β1(TGF-β1). Method: The HK-2 cells were cultured by DMEM/F12(1:1) with 10% fetal bovine serum and divided into control group, TGF-β1 group (TGF-β110 μg·L-1), rat serum control group(TGF-β110 μg·L-1 +10% rat serum), TNK -containing rat serum therapy groups (TGF-β110 μg·L-1 + 20% TNK or + 10% TNK or + 5% TNK). After 24 h, the expression of transforming growth factor β receptor I(TβRI), transforming growth factor β receptor II (TβRII) mRNA were tested by fluorescence quantitatiye PCR assay, and the expression of Smad 2, Smad 3 protein by Western-blot assay. Result: The expression of TβRI, TβRII mRNA and Smad 2, Smad 3 protein of HK-2 cultured with TGF-β1 were much notable than the control, and significantly decreased in HK-2 cultured with TGF-β1 plus TNK compared with only TGF-β1 (P<0.05), but only rat serum had no such effect. Conclusion: TNK could regulate the smads signaling pathway of human renal tubular epithelial cells HK-2 induced by transforming growth factor-β1, and could prevent the development of renal fibrosis to a certain extent.关键词：Tangnaikang;transforming growth factor-β1;HK-2 cell;transforming growth factor β receptor I;transforming growth factor β receptor II;Smad 2;Smad 3
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of meth-ferulic acid (MFA) on the proliferation and expression of α-smooth muscle actin (α-SMA) in transforming growth factor beta 1(TGF-β1)-induced human hepatic stellate cells (HSC-LX2). Method: TGF-β1 5×107/L induced human hepatic stellate cell line (HSC-LX2) in vitro were cultured with different dose of MFA(the final concentration being 5, 10, 20 mg·L-1). MTT method was used to detect the proliferation of TGF-β1-induced HSC-LX2. Western blotting and RT-PCR were used to detect the expression of α-smooth muscle actin (α-SMA) of HSC-LX2. Result: MFA could inhibit the proliferation of TGF-β1-induced HSC-LX2 with a dose dependent, the inhibitory effect by 5 mg·L-1 concentration was more obvious, compared with the TGF-β1-induced the model group(P<0.05);and compared with TGF-β1-induced the model group, the expression of α-SMA mRNA and protein by 5 mg·L-1concentration was more obvious. Conclusion: MFA pretreatment on human hepatic stellate cells can reduce α -SMA mRNA and protein levels.关键词：meth-ferulic acids;transforming growth factor-β1;hepatic fibrosis;human hepatic stellate cells
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发布时间：2024-01-04 - 摘要：Objective: To measure polysaccharide content in different parts of Camellia chrysanthaand explore the differences in antioxidant activity in vitro. Method: Extract polysaccharides from different parts of C. chrysantha by ultrasonic extraction, phenol-sulfuric acid method was used to determine polysaccharides content and measure antioxidant capacity in different parts of Camellia water extract by ABTS method, DPPH assay, pyrogallol method and phenanthroline assay. Result: C. chrysantha flowers, leaves, bud tip, husk polysaccharide contents were 32.88, 29.48, 35.89, 30.02 g·kg-1.The ability to clear ABTS+·order was ascorbic acid (0.100 g·L-1) flowers (0.135 g·L-1) > nutshell (0.165 g·L-1) > bud tip (0.243 g·L-1) > leaf (0.330 g·L-1), the ability to clear DPPH order was Ascorbic acid (0.200 g·L-1) > flowers (0.344 g·L-1) > nutshell (0.435 g·L-1) > bud tip (0.881 g·L-1) > leaf (1.011 g ·L-1). The ability of clear Superoxide anion radical order was Ascorbic acid (0.2 g·L-1) ≥ bud tip (25.0 g·L-1) > nutshell (25.0 g·L-1) > flowers (25.0 g·L-1) > leaves (25.0 g·L-1). When crude drug concentration was 25 g·L-1, the flowers, the cusp, nutshell elimination rate of hydroxyl radicals were greater than 50%, however all were less than ascorbic acid (0.2 g·L-1).The comprehensive score of bud tip (55.05) > flowers (52.79) > nutshell (51.97) > leaf (23.73). Conclusion: Phenol-sulfuric acid colorimetry polysaccharide content in different parts of C. chrysantha was viable and stable and aqueous extract of C. chrysantha has a strong antioxidant capacity, the antioxidant capacity are significant differences and depend on different parts of C. chrysantha.关键词：Camellia chrysantha;different fractions;polysaccharides;content determine;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects and its mechanismof Xiebai San on allergic asthma. Method: Sixty BALB/c were divide into six groups:normal group, model groups, Xiebai San high dose(12 g·kg-1), middle dose(6 g·kg-1), low dose(3 g·kg-1), dexamethasone positive group [(2 mg·kg-1), groups mice (except those in the normal group) were sensitized with 0.2 mL sensitized solution(0.2 mL sensitized solution contains 0.1 mg OVA and 0.02 mg Al(OH)3)].Fifteen day after modeling , and corresponding drugs were given , 30 min later, mice were stimulated with 5% OVA. Blood simples were collectesd for counting numbers of eosinophilic granulocyte(EOS), and bronchoalveolar laverge fluid(BALF)was used for counting inflammatory cells.ELISA was used to assay interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) levels , Western blotting was Use to detect GATA3 and T-bet. Result: The middle dose of Xiebai San could obviously reduce numbers of EOS in the blood (P<0.01), high dose and low dose of Xiebai San could also reduce numbers of EOS(P<0.05), high and middle dose of Xiebai San could obviously reduce numbers of EOS in the BALF(P<0.01), low dose Xiebai San could also reduce numbers of EOS(P<0.05), and Xiebai San obviously reduced IL-6 and TNF-α, and GATA3, and increased T-bet. Conclusion: Xiebai San can inhibit allergic aasthma.关键词：Xiebai San;allergic asthma;GATA-binding protein-3;T-box expressed in T cell
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of the Xinjiangtang granule on the serum levels of insulin, superoxide dismutase (SOD), malondialdehyde (MDA), C-reactive protein(CRP) and the expression of hepatic glucokinase (GK) mRNA in type 2 diabetes mellitus (T2DM) rats, and to explore its anti-diabetic mechanisms. Method: SD rats were randomly divided into normal group and diabetic group. The model of T2DM rats were established by feeding with high fat diet and injecting low dosage of streptozotocin (STZ) at 28 mg·kg-1. Then the rats in diabetic group were randomly divided into model group, metformin group (0.15 mg·kg-1), and low-, medium-, high-dosage groups of Xinjiangtang granule (22.8, 11.4, 5.7 g·kg-1). The rats in normal and model groups were given the same dosage of normal saline. All rats received oral administration of corresponding medicines for 5 weeks. The levels of fosting blood glucose(FBG) were assayed by oxidase method. The serum levels of fasting insulin(FINS) and CRP were assayed by ELISA. The serum levels of SOD and MDA were detecteded by assay kits. The expression of GK mRNA was detected by RT-qPCR. Result: Compared with the normal group, the levels of FBG, insulin resistance index(IRI), FINS, CRP showed obvious increase (P<0.01), and serum SOD level showed an obvious decrease in the model group (P<0.01). The levels of FBG, IRI, MDA, CRP of the high-, medium- and low-dosage Xinjiangtang granule group showed obvious decrease (P<0.01), the levels of FBG, IRI, FINS, CRP showed obvious decrease (P<0.05, P<0.01)in the low-dosage Xinjiangtang granule group, and serum SOD level showed an obvious increase as compared with those in model group (P<0.05, P<0.01). The expression of hepatic GK mRNA showed an obvious decrease in model group as compared with that in normal group(P<0.05). While it showed an obvious increase in medium-and low-dosage Xinjiangtang granule group as compared with that in model group(P<0.01). Conclusion: The Xinjiangtang granule can significantly reduce the blood glucose level in T2DM rats, which may be achieved by reducing FINS level, enhancing the capacity of resistance to oxidative stress, relieving inflammation and improving the hepatic GK mRNA level.关键词：diabetes;Xinjiangtang granule;oxidative stress;insulin resistance;glucokinase
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发布时间：2024-01-04 - 摘要：Objective: To explore the mechanisms of Huazhuo Jiedu recipe in treating reflux esophagitis (RE). Method: Sixty male Wistar rats were randomly divided into control group, model group, omeprazole group, Huazhuo Jiedu recipe group of fifteen rats each group. The RE model was established using partial pyloric ligation and cardiomyotomy except the rats in control group. The rats in control and model groups were intragastrically administrated with distilled water. The rats in omeprazole group were intragastrically administrated with 3.6 mg·kg-1 omeprazole. The rats in Huazhuo Jiedu recipe group were intragastrically administrated with 12.5 g·kg-1 Huazhuo Jiedu recipe. The histopathology in esophagus tissues was observed after 8 weeks treatment. Meanwhile, the content of gastrin(GAS), motilin(MTL) and vasoactive intestinal peptide(VIP) in serum were tested by ELISA. Result: Omeprazole and Huazhuo Jiedu recipe could improve the pathological changes of esophageal mucosa, there were statistically significant difference as compared with model group (P<0.05). Huazhuo Jiedu recipe could increase contents the GAS, MTL, decrease the content of VIP, there were significant difference as compared with model group (P<0.05). Conclusion: Huazhuo Jiedu recipe has effect on RE rats, which may be chieved by increasing the contents of GAS and MTL, reducing the content of VIP in serum.关键词：Huazhuo Jiedu recipe;reflux esophagitis;gastrin;motilin;vasoactive intestinal peptide
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect on diabetic peripheral neuropathy of DL-thioctic acid combined with the Chinese medicine foot massage under the guidance of dynamics-immobility-order-succession and eight methods therapy. Method: Seventy patients were enrolled according to the inclusion and exclusion criteria in this study. Then the patients were randomly divided into the control group and the observation group. The patients in both groups received basis treatment and patients in observation group received Chinese medical foot massage for two two-weeks courses. Scores of Chinese medical symptom, neural symptoms and signs were graded before and after treatment. The ankle-brachial index (ABI) in lower extremity arterial blood flow, common peroneal nerve conduction velocity including motor conduction velocity (MCV) and sensory conduction velocity (SCV) were evaluated before and after treatment. The adverse reactions of treatment were carefully monitored in this study. Result: Scores of Chinese medical symptom, neural symptoms and signs in observation group were lower than that in control group after treatment (P<0.05). Scores of ABI, MCV and SCV were significantly higher in observation group as compard with that in control group (P<0.05). No serious adverse reactions were found. Conclusion: DL-thioctic acid combined with Chinese medicine foot massage under the guidance of dynamics-immobility-order-succession and eight methods therapy can improve the clinical symptoms and the lower extremity arterial blood flow in patients with diabetic peripheral neuropathy, which provide a new method for treatment of diabetic peripheral neuropathy using Chinese medicine.关键词：dynamics-immobility-order-succession and eight therapies;DL-thioctic acid;diabetic peripheral neuropathy;foot massage;promoting blood circulation and removing blood stasis
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发布时间：2024-01-04 - 摘要：Objective: To observe the safety and efficacy of cytokine-induced kill(CIK) cells combined with cisplatin and traditional Chinese Medicine(TCM) in treating patients with malignant pleural effusion (MPE). Method: A prospective randomised single-blind study was performed, seventy-three MPE patients who meet the inclusion criteria were divided into the experimental group and the control group. Patients in both groups received conventional central venous catheter drainage for pleural effusion. Thirty-six patients in experimental group received thoracic perfusion with cisplatin and 2 weeks later added CIK cellsonce daily for 3 days.Thirty-seven patients received thoracic perfusion with cisplatin alone.Patients in both groups took orally Wenfei Huayin recipe for 4 weeks.The disease control rate (DCR)of MPE, symptoms score and Karnofsky performance status (KPS) score were evaluated after the treatment.T lymphocyte subsets was analysised and adverse reactions were carefully monitored before and after the treatment. Result: The DCR of MPE was 95.0% in the experimental group and that incontrol group were 82.0% with statistically significant differences(P<0.05). Scoresof symptoms and KPS in experimental group were superior to that in control group(P<0.05).The CD3+T, CD4+T, CD8+T and NK lymphocytesinboth groupsshowed an increase after the treatment, and there was statistically significant differences for CD3+T and NK lymphocytesin experimental group (P<0.05).Less adverse reactions were found in experimental group than that in control group. Conclusion: Thoracic perfusion with CIK cells combined with cisplatin and TCM canreduce watery phlegm, increase scores of symptoms and KPS, boost the immune systemand improve the quality of life in MPE patients. Meanwhile, it has good safty and few adverse reactions. Therefore, it is worthy to be applied widely in clinic.关键词：malignant pleural effusion;cytokine induced kill cells;Wenfei Huayin recipe
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发布时间：2024-01-04 - 摘要：Objective: To investigate and analysis the clinical curative effect and the clinical value of modified Shashen Maidong tang combined with chemotherapy treatment for patients with locally advanced non-small cell lung cancer (NSCLC). Method: Sixty-eight patients were enrolled and randomly divided into control group and observation group.Patients in both group received conventional chemotherapy treatment, and patients in observation added modified Shashen Maidong tang. The improvementin quality of life, stablility of lesions and indexes of therapeutic effectwere compared between two groups. Result: Therewas no significant differencein quality of lifescore between the two groups before the treatment.While the quality of life score, the stability rate of lesions and the total curative rate showed significant improvement after the treatment, and observation group was superior to control group (P<0.05). Conclusion: Clinical application of Shashen Maidong tang combined chemotherapy treatment has remarkable curative effect in patients with NSCLC in terms of improving the survival and quality of life, and this method has certain clinical value.关键词：middle-late stage non-small cell lung cancer;Shashen Maidong tang;chemotherapy;clinical curative effect analysis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the safety and effectiveness of Gangdi Pingchuan decoction in the treatment of patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). Method: Seventy-nine patients according with the standard diagnosis of AECOPD and lung distension (phlegm and blood stasis of TCM syndrome differentiation standard) were randomly divided into control group (40 cases)and observation group(39 cases). Patients in both group received conventional western medicine treatment. Patients in observation group added one dose Gangdi Pingchuan decoction enema daily for 1 week. Changes of TCM symptom scores, pulmonary function, arterial blood gas analysis and safety indexes were observed before and after treatment. Result: The improvement in TCM clinical symptoms of shortness of breath, anorexia and bloating, constipation in the observation group were better than those in the control group (P<0.01). The pulmonary ventilation function, forced vital capacity (FVC)as a percentage of predicted value(FVC%) of patients in observation group were significantly improved as compared with that in control group(P<0.05).Partial pressure of oxygen and blood oxygen saturation had an increase, partial pressure of carbon dioxide had a decrease in observation group, which is superior to those in control group(P<0.05).The observation group had good curative effect in control infection than the control group(P<0.05).There were no apparent effects on liver and renal function after 1-week treatment. Conclusion: Gangdi Pingchuan decoction can improve the therapeutic effect of western medicine in patients with AECOPD and no apparent effects on liver and renal function were found. The regimen of Gangdi Pingchuan decoction plus western medicine is superior to western medicine alone, which may be worth further study.关键词：Gangdi Pingchuan decoction;acute exacerbation of chronic obstructive pulmonary disease;enema
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical curative effect of traditional Chinese medicine Fufang Luobuma pills combined with amiodarone in the treatment of ventricular arrhythmias coronary heart disease (VACHD). Method: One hundred and twenty-five patients with VACHD were randomly divided into three groups. Forty-five patients in the treatment group received treatment of Fufang Luobuma pills combined with amiodarone, forty-five patients in amiodarone group received treatment of amiodarone alone, and thirty-five patients in the control group received treatment of propafenone alone. The efficacy and adverse reaction of treatment were compared among the three groups. Result: After treatment, the total effective rate in the treatment group, the amiodarone group and the control group were 95.6%, 86.7%and 74.3%, respectively, with statistically significant differences between each other groups (P<0.05). The premature ventricular number, nonsustained ventricular tachycardia were obviously improved in the three groups after treatment, and the ventricular premature number, nonsustained ventricular tachycardia in the treatment and amiodarone groups were lower than those in the control group with statistically significant difference (P<0.05). The premature ventricular number, nonsustained ventricular tachycardia number in the treatmentgroup were lower than that in amiodarone group with statistically significant difference (P<0.05). Conclusion: Using Fufang Luobuma pills combined with amiodarone and amiodarone alone have efficacy in treating patients with VACHD, and the combination therapy is better and safer than amiodarone alone. It can be the preferred choice in the treatment of VACHD because of low price, convenient administration route and satisfactory curative effect, which is worthy to be applied widely in clinic.关键词：Fufang Luobuma pills;amiodarone;coronary heart disease;ventricular arrhythmia
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical short-term curative effect and the mechanism of Tongbi Zhitong decotion combined with acupuncture in patients with sciatica of nerve root. Method: One hundred and twenty patients were randomly divided into control group and observation group of 60 patients each. Patients in control group received acupuncture treatment alone. Patients in observation group received differentiated treatment of Tongbi Zhitong decotion combined with acupuncture. All patients received two 10 days periods of treatment separated by 2 days without treatment. Pain degree on visual analog scale (VAS), Oswestry disability index and Japanese orthopaedic association (JOA) score were evaluated before and after treatment. Levels of β-endorphin(β-EP), interleukin-1 (IL-1), interleukin-1 (IL-6) and tumor necrosis factor-α (TNF-α) were detected. Result: The total clinical effective rate in observation group was 95%, superior to 81.6% in control group (P<0.05). The VAS scores in both groups exhibited a declining tendency at 3, 10 and 22 day after the treatment(P<0.01). The VAS scores in observation group were lower than that in control group at 10, 22 day after the treatment (P<0.01). The oswestry dysfunction index in observation group was worse than that data in control group, while JOA scores in observation group were superior to that in control group (P<0.01). The level of β-EP in observation group was higher (P<0.01), while the levels of IL-1, IL-6 and TNF-α were lower in observation group than that in control group (P<0.01). Conclusion: Compared with the treatment using acupuncture alone, acupuncture combined with medicine therapy has significant improvements in pain relieving, functional statue and clinical effective rate for patients with sciatica of nerve root. Its mechanisms may be achieved by raising β-EP level and reducing inflammatory reaction.关键词：sciatica;acupuncture;Tongbi Zhitong decotion;β-endorphin;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effect of extract of ginkgo biloba injection combined with donepezil in treating vascular dementia (VD) and to explore its effect on anti-oxidation activity, vascular endothelial function and cerebral hemodynamics. Method: Seventy patients with VD were randomly divided into control group and observation group of 35 patients each by random number table. Patients in control group took 10 mg donepezil hydrochloride tablets orally daily for 3 months. Based on the treatment of control group, patients in observation group added extract of ginkgo biloba injection intravenous drip, 20 mL/time, twice daily for six 10 days courses of treatment separated by 5 days without treatment. Scores of activities of daily living (ADL), digital simultaneous voice and data (DSVD) and mini-mental state examination were graded before and after treatment. Levels of serous endothelin (ET), nitric oxide (NO), superoxide dismutase (SOD) and malonaldehyde (MDA) were detected. Average blood velocities of arteria cerebri anterior (ACA) and middle cerebral artery (MCA) pulsatility index were detected by transcranial doppler. Result: The total effective rate of cognitive ability in observation group was 77.14%, which is superior to that in control group 51.43%(P<0.05). The total effective rate of activity of daily living in observation group was 80%, which is superior to that in control group 57.14%(P<0.05). Grades of ADL and MMSE in observation group were higher, and grade of DSVD was lower than those in control group after treatment (P<0.01). Levels of NO, ET and MDA in observation group were lower than those in control group, level of SOD was higher than that in control group (P<0.01). Average blood velocities of ACA and MCA in observation group were higher than those in control group (P<0.01). ACA and MCA pulsatility index in both groups had a decreasing trend, but there was no statistically significancent difference. Conclusion: Extract of ginkgo biloba injection combined with donepezil can improve the ability of living and understanding of patients with VD. Its mechanism of action may be relevant to ameliorating endothelial function, anti-oxidation and preventing free radical damage, promoting cerebral hemodynamics.关键词：vascular dementia;ginkgo biloba extract injection;Donepezil hydrocloride tablets;endothelin;nitric oxide;superoxide dismutase;Malonaldehyde
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical curative efficacy of Hugan Jiangzhi decoction and the influence on levels of serous adiponectin, leptin and superoxide dismutase(SOD) in patients with non-alcoholic fatty liver disease(NAFLD). Method: Ninety-eight patients with NAFLD were randomly divided into control group and observation group of 49 patients each by random number table.Patients in control group received metformin hydrochloride tablets (0.25 g orally 3 times daily), lovastatin tablets (20 mg orally once daily), and polyene phosphatidylcholine capsules (2 capsules orally 3 times daily).Based on the treatment of control group, patients in observation group added modified Hugan Jiangzhi decoction 1 dose daily.The patients in both groups received two 12 weeks periods of treatment.Patient underwent abdominal computed tomography (CT) before and after treatment, and liver/spleen CT ratio was calculated.Levels of alanineaminotransferase(ALT), aspertate aminotransferase(AST), triglyceride(TG), total cholesterol(TC), high density lipoprotein-cholesterol(HDL-C) and low density lipoprotein-cholesterol(LDL-C) were detected.Levels of serum adiponectin, leptin and superoxide dismutase (SOD) were detected.Body mass index(BMI), waist hip rate and homeostasis model assessment of insulin resistance(HOMA-IR) were measured before and after treatment. Result: The total effective rate in observation group was 95.92%, which is superior to that in control group(81.63%, P<0.05).The liver/spleen CT ratio was higher in observation group than that in control group after treatment(P<0.05).The levels of ALT, AST, TC, TG and LDL-C were lower, and the level of HDL-C was higher in observation group than those in control group(P<0.01).The SOD and adiponectin level were higher, leptin level was lower in observation group than those in control group after treatment(P<0.01).The BMI and waist, hip circumference in both two groups after treatment increased as compared with those before, but there was no statistically significant difference between two groups.IR levelin observation group was lower than that in control group after treatment (P<0.01). Conclusion: Hugan Jiangzhi decoction haseffectsonliver protection and lipid-loweringin patientswith NAFLD.Beside it has obvious effect in improving NAFLD and increasing clinical curative effect.The mechanism may be achieved by regulating inflammatory cytokines, increasing antioxidant capacity and improving IR of patients.关键词：non-alcoholic fatty liver disease;Hugan Jiangzhi decoction;insulin resistance;superoxide dismutase;adiponectin;leptin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the curative effect of Kuntai capsules and the influence on serum sex hormone and blood fat in patients with climacteric syndrome(CS). Method: Eighty-six patients with CS were randomly divided into control group and observation group of 43 patients each by random number table. Patients in both groups received basic treatment including health education, psychological guidance, dietary guidance, sport coaching, etc.Patients in control group took 2.5 mg tibolone tablets orally once daily. Patients in observation group took 4 Kuntai capsules orally 3 times daily. The patients in both groups received two 12 weeks periods of treatment. Grades of Kupperman(KI) and liver-kidney yin deficiency were recorded before and 4, 8 and 12 weeks after the treatment. Levels of serous estradiol2(E2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), triglyceride(TG), total cholesterol(TC), high density lipoprotein-cholesterol(HDL-C) and low density lipoprotein-cholesterol(LDL-C) were detected before and after treatment. Result: The total curative ratewas 86.04%in observation group and 81.39% in control group with no significant difference between two groups.Scores of KI in both groups decreased(P<0.01) at 4, 8 and 12 weeks after treatment, but there was no significant difference from each other. Score of liver-kidney yin deficiency in observation group was lower than that in control group(P<0.01). Level of E2 in both groups increased after treatment than before (P<0.01), and levels of FSH, LH decreased(P<0.01)at 4, 8 and 12 weeks after treatment, but there was no significant difference from each other. TG, TC, LDL-C in observation group were lower, and HDL-C was higher than those in control group after treatment(P<0.05). The people who have normal TG, TC, LDL-C and HDL-C levels in observation group were more than those in control group(P<0.05). Conclusion: Kuntai capsules has the same curative effect as tibolone tablets in improving clinical symptomsand treatment of patients with CS, it has higher efficacy than tibolone tablets in improving estradiol leveland regulating lipid metabolism.关键词：climacteric syndrome;Kuntai capsules;blood fat;sex hormone
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发布时间：2024-01-04 - 摘要：Objective: The bacteriostatic effects of 53 Chinese antipyretic herbs against pseudomonas aeruginosa and their antibacterial difference were classified and confirmed by cluster analysis in order to instruct clinical administration. Method: Antibacterial effect of traditional Chinese medicine was ascertained by determination of diameter of inhibition zone K-band in liquid culture. Result: The cluster analysis showed that the classification of bacteriostatic Pseudomonas aeruginosa effects were not consistent with traditional classification of Chinese antipyretic herbs group. Conclusion: For the clinical prevention of P. aeruginosa infection, the optional Chinese antipyretic herbs were suggested as paeoniae radix rubra, picrorhizae rhizoma, coptidis rhizoma, taraxaci herba, luffae fructus retinervus, scutellariae radix, buddlejae flos, etc.关键词：Chinese antipyretic herbs;pseudomonas aeruginosa;antibacterial effect;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To analyze the composing principles of herbs in the prescriptions for treating cerebral ischemia-reperfusion injury. Method: In the prescriptions for experimentally treating cerebral ischemia-reperfusion injury in China National Knowledge Infrastructure (CNKI) were collected and a database was set up, then by using traditional Chinese medicine (TCM) inheritance system inherent the composing principles of herbs in the prescriptions were analyzed. Result: Based on the analysis of 137 cases of the prescriptions in the database , the frequency of each herb appeared in the database was computed, combination of herbs, associations principles and relationship framework between herbs were mined from the database. Conclusion: Composing principles of herbs in the prescriptions for treating cerebral ischemia-reperfusion injury be showed by TCM inheritance system.关键词：cerebral ischemia-reperfusion injury;the Chinese medicine heritage auxiliary system;prescription law
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发布时间：2024-01-04 - 摘要：To provide new ideas for the technology and equipment development by means of reviewing the research status of the vacuum extraction from traditional Chinese medicine(TCM). Through analysis and statistics of the literature, contrast vacuum extraction process with other extraction technology, analyses the advantage and problems of the vacuum extraction, providing thought for the application of vacuum extraction equipment and the development and improvement. Vacuum extraction can be extracted in lower temperature at the same time guarantee the dynamic, improved the extraction yield of heat sensitive drugs, decrease types of impurities dissolution, etc. Vacuum extraction in the application of TCM compound and the drug efficacy of the vacuum extraction should be further research. Vacuum extraction has unique advantages in the TCM extraction, based on the characteristics of drug itself, selecting suitable vacuum extraction process, and can be applied to practical production.关键词：vacuum extraction;principles;adaptability;industrialization;pharmaceutical equipment
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发布时间：2024-01-04 - 摘要：As traditional Chinese medicine industry played more and more important role in national economy, many researchers changed their focuses on traditional Chinese medicine industry chain. After literature review of definition, existing questions, organizational model, construction and optimization, and industry cluster, it was found that existing researches mostly applied theory of industry chain into traditional Chinese medicine industry from perspective of theory, few conducted detailed studies considering the modernization of traditional Chinese medicine industry and traditional Chinese medicine industry chain characteristic. Existing research lacked of the traditional Chinese medicine industry chain optimization and integration of research from the perspective of the whole industry chain. This paper pointed out that research of the traditional Chinese medicine industry chain formation cause and mechanism, evolution path and method still remained research blank. Future research should focus on industry chain theory and traditional Chinese medicine industry chain characteristic to explore the unique operation mechanism and resource allocation mode, regional traditional Chinese medicine industry chain development status, strategic choice and optimal path, and empirical research of traditional Chinese medicine industry chain.关键词：traditional Chinese medicine industry;industrial chain;definition;organizational model;literature review
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发布时间：2024-01-04 - 摘要：Objective: Chemical constituents of Goniothalamus genus involved summarized by retrieval literature;main chemical structure types for Goniothalamus gens involved styrene lactones, alkaloids, flavonoids, acetogenins, and essential oil;and 51 styrene lactones, 40 alkaloids, 10 flavonoids, 4 acetogenins were isolated from Goniothalamus genus;to provide a clue for the modern research work, this paper reviewed progress on chemical constituents of Goniothalamus genus.关键词：Goniothalamus;chemical constituents;styrene lactones;alkaloids;flavonoids;acetogenins
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