最新刊期
卷 20 ， 期 19 ， 2014
- 摘要：Objective: To optimize formulation process of ursolic acid solid lipid nanoparticles and investigate its physicochemical properties and antitumor activity. Method: Ursolic acid solid lipid nanoparticles was prepared by film dispersion method,orthogonal test was adopted to optimize formulation process by taking encapsulation efficiency as index.Its physical and chemical properties were studied by transmission electron microscope and laser particle sizer/Zeta potential system,MMT was used to detect its in vitro anti-tumor activity. Result: The best formulation process was as following:ursolic acid 90 mg,lecithin 30 mg,stearic acid 10 mg,PBS concentration of 10 mmol·L-1.These prepared nanoparticles showed spherical or ellipsoidal under transmission electron microscope,average particle size,encapsulation efficiency and drug loading were (184.7±18.3)nm,(82.6±0.6)% and (11.9±0.5)%,respectively;release was 81.2% at 48 h,particle size and encapsulation efficiency had no significant change in 30 d.When the concentration of ursolic acid were 5,10, 20, 40, 80 μmol·L-1,inhibition rates of ursolic acid on human hepatoma cell line SMMC-7721 were (9.0±1.2)%,(15.7±2.8)%,(42.3±4.6)%,(78.7±6.9)% and (79.3±7.2)%,inhibition rates of ursolic acid solid lipid nanoparticles were (9.0±1.3)%,(23.6±2.2)%,(59.3±6.1)%,(84.7±8.3)% and (85.0±8.1)%,respectively. Conclusion: These prepared ursolic acid solid lipid nanoparticles have appropriate size,good in vitro release and stability with excellent antitumor activity.关键词：ursolic acid;lipid nanoparticles;formulation process;physicochemical properties;antitumor activity
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To prepare CZ48 sustained-release nanoparticles and investigate its in vitro release. Method: Using polylactic acid(PLA) as material,CZ48 sustained-release nanoparticles was prepared through W/O/W double emulsion technique,its micromorphology was observed by transmission electron microscope,particle size was determined by particle size analyzer and in vitro release was investigated. Result: CZ48 sustained-release nanoparticles showed obvious spherical structure with particle size of (260.6±3.2)nm,encapsulation efficiency of (88.7±2.55)% and drug-loading of (11.8±1.29)%,in vitro release studies showed that 85% of CZ48 was released from sustained-release nanoparticles in the first 48 h. Conclusion: These prepared CZ48 sustained-release nanoparticles are successfully prepared through W/O/W double emulsion technique with satisfactory encapsulation efficiency and drug-loading and obvious sustained-release,which provides data support for further research on pharmacokinetic.关键词：CZ48;sustained-release nanoparticles;polylactic acid;in vitro release
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare different processing technologis of steamed Cibotii Rhizoma and optimize its steaming process. Method: HPLC was employed to determine contents of protocatechuic acid and protocatechuic aldehyde with mobile phase of methanol-1% acetic acid(5: 95) and detection wavelength at 280 nm.Taking composite score of contents of protocatechuic acid and protocatechuic aldehyde as indicator,compare among processing technologies of cutting after whole steamed,steaming after freshly cut,steaming after drying the fresh slices,steaming after the fresh slices heavily oxidized and so on.Then orthogonal design was adopted to optimize steaming process with infiltration time,steaming time and moistening time as factors. Result: It is reasonable processing method to be steamed after drying the fresh slices,optimal processing technology was as following:infiltrated 1 h at ambient temperature,steamed by high-heat steam for 4 h and then moistened for 4 h;mass fractions of protocatechuic acid and protocatechuic aldehyde were 1.561,0.107 mg·g-1,respectively. Conclusion: This optimized processing technology is reasonable and practical,which can provide a reference for processing in produciton place of Cibotii Rhizoma.关键词：Cibotii Rhizoma;protocatechuic acid;protocatechuic aldehyde;steaming-cutting process
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish odor fingerprint by electronic nose technology and distinguish different processed products of Alpiniae Oxyphyllae Fructus. Method: Crude,stir-frying with water and salt products of Alpiniae Oxyphyllae Fructus were detected by electronic nose technology,then response values were obtained by the sensors,data was analyzed by chemometrics methods,such as principal component analysis,discriminant factor analysis,statistical quality control analysis. Result: The crude,stir-frying with water and salt products could be distinguished from each other obviously through chemometrics analysis.Odor of the crude was quite different from its processed products,odor of stir-frying with water and salt products was similar. Conclusion: Electronic nose technology can be used for distinguishing processed products of Alpiniae Oxyphyllae Fructus,this study indicate that odor changes of Alpiniae Oxyphyllae Fructus mainly caused by heating up.关键词：Alpiniae Oxyphyllae Fructus;electronic nose;stir-frying with water products;stir-frying with salt products;odor fingerprint
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To prepare Prpnephrium triphyllum-Rheum officinale solid lipid nanoparticles and investigate its morphology. Method: HPLC was adopted to determine contents of emodin and triphyllin A with mobile phase of acetonitrile-0.1% formic acid-water for gradient elution and detection wavelength at 226 nm.Film-ultrasonic wave dispersion technique was used to prepare P. triphyllum-R.officinale solid lipid nanoparticles,on the basis of single factor tests,orthogonal test was employed to optimize preparation technique by taking entrapment efficiencies of emodin and triphyllin A as comprehensive evaluation index. Result: Optimal formulation was as following:extract of P. triphyllum-R.officinale 150 μL,stearic acid-soybean lecithin (1: 1),tween-80 of 1.0%;mean particle size diameter was (301±2.8) nm,Zeta potential was (-19.94±3.82)mV,entrapment efficiencies of triphyllin A and emodin was 61.8% and 81.2%,respectively. Conclusion: These prepared P. triphyllum-R.officinale solid lipid nanoparticles show spherical,this preparation process is stable and feasible.关键词：Pronephrium triphyllum;Rheum officinale;solid lipid nanoparticles;emodin;film-ultrasonic wave dispersion technique;triphyllin A
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize flash extraction process of active components from Eucommiae Folium. Method: HPLC was employed to determine contents of chlorogenic acid and geniposidic acid with mobile phase of methanol-water-glacial acetic acid(15: 85: 1),flow rate of 1.0 mL·min-1 and detection wavelength at 237 nm.Taking yields of chlorogenic acid and geniposidic acid as index,based on single factor tests,orthogonal test was adopted to optimize extraction process with ethanol concentration,liquid-solid ratio,extracting time and times as factors. Result: Optimal process conditions were as follows:extracted thrice with 25 times the amount of 70% ethanol for 6 min each time;yields of chlorogenic acid and geniposidic acid were 1.82% and 0.47%,respectively. Conclusion: This optimized process is simple,accurate and suitable for extracting active ingredients from Eucommiae Folium.关键词：Eucommiae Folium;chlorogenic acid;geniposidic acid;flash extraction
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro release law and mucosal permeability mechanism of linarin in Chrysanthemi Indici Flos total flavonoids bioadhesive bilayer sustained-release patches. Method: Taking linarin as index components,in vitro release law of total flavonoids bioadhesive bilayer sustained-release patches was investigated;abdominal skin of bullfrog was adopted,mucosal permeability mechanism of this preparation was explored through transdermal diffusion test. Result: Equations of in vitro release and transdermal diffusion were fitted lnQ=1.387lnt-0.461(r=0.990) and lnQ=0.969lnt+3.240(r=0.994 3),which were in line with Ritger-Peppas equation.Release system of this preparation in release medium was synergy results of Fick diffusion and gel matrix erosion. Conclusion: These prepared patches have good sustained-release and a certain mucosal permeability.关键词：total flavonoids from Chrysanthemi Indici Flos;bioadhesive preparation;bilayer sustained-release patches;in vitro release;mucosal permeability
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Pinelliae Rhizoma Praeparatum Cum Alumine. Method: Taking mass fractions of extract,total acids and Alumen as indexes,orthogonal test was adopted to optimize processing technology with mass ratio of herbs to Alumen,soaking time and decocting time as factors. Result: Optimum processing technology was that soaked 2 hours,boiled 6 hours,ratio of herbs to Alumen of 10: 1;mass fractions of extract,total acids and Alumen were 0.83%,10.5% and 6.4%,respectively. Conclusion: Contents of extract,total acids and Alumen are in line with requirements of Pinelliae Rhizoma Praeparatum Cum Alumine in the 2010 edition of‘Chinese Pharmacopoeia’,calcium oxalate crystals significantly reduce,this study show that water decoction process can reduce toxicity and irritation of Pinelliae Rhizoma with a relatively short time by comparing with conventional processing technology.关键词：Pinelliae Rhizoma Praeparatum cum Alumine;total acid;extract;Alumen
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of compound Niuhuang capsules. Method: UV was employed to determine the content of total saponins with detection wavelength at 560 nm and 545 nm.Taking quality of total saponins as index,orthogonal test was adopted to optimize water extraction process with the amount of water,soaking time,extration time and times as factors;the same method was used to investigate effects of ethanol concentration,ethanol amount,extraction time and times on alcohol extraction process. Result: The best water extraction process was:soaked 0.5 h with 10 times the amount of water,decocted two hours for three times;optimum alcohol extraction process was as following:decocted thrice with 8 times the amount of 70% ethanol for 2 h each time. Conclusion: These extraction process is stable and feasible,this study can provide an experimental basis for clinical application of compound Niuhuang capsules.关键词：compound Niuhuang capsules;total saponins;astragaloside;ginsenosides Rg1
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate effects of steaming time and baking time on contents of six kinds of lignans from Schisandrae Chinensis Fructus in processing by vinegar in order to improve its processing parameters. Method: HPLC was employed to determine contents of six kinds of lignans(schizandrol A,schizandrol B,schisantherin A,schizandrin A,schizandrin B and schizandrin C) with mobile phase of acetonitrile(A)-water(B) in gradient elution (0-17 min,50%A;17-25 min,50%-55%A;25-30 min,55%-75%A;30-55 min,75%A;55-65 min,75%-50%A) and detection wavelength at 254 nm.Effects of steaming time and baking time on content of lignans from Schisandrae Chinensis Fructus in vinegar processing were investigated by single factor tests. Result: Steaming time and baking time had a certain effects on contents of six kinds of lignans in processing,optimum processing technology was as following:moistened 1.5 h with 20% times the amount of vinegar,steamed 3 h,baked 11 h at 50 ℃;mass fractions of six kinds of lignans were 0.524%,0.151%,0.085%,0.125%,0.185% and 0.046%,respectively. Conclusion: After being processed with vinegar,in addition to the content of schisantherin A increases slightly,the other five kinds of lignans are down slightly without significant difference.Schisandrae Chinensis Fructus can not bake a long time after being steamed.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare decoction efficiency of compressed pieces and traditional pieces of Epimedii Folium in different time points. Method: Traditional decoction extraction was adopted,taking the content of icariin and dry extract yield as comprehensive evaluation index,decoction efficiency of traditional pieces and compressed pieces in different times was compared and f2similarity factor evaluation was investigated. Result: The content of icariin and dry extract yield in compressed pieces were a little higher than traditional pieces in the single decoction and compound decoction without significant difference,f2=97,their decocting amount of icariin were 4.101,3.498 mg·g-1 when boiled 60 min. Conclusion: Quality of Epimedii Folium do not change after being compressed,which can not affect decoction of active ingredients,it provide new ideas for application form innovation of traditional Chinese medicine pieces.关键词：Epimedii Folium;compressed pieces;decoction efficiency;icariin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasonic-assisted extraction process of total flavonoids from Clinacanthus nutans and evaluate its in vitro antioxidant activity. Method: Based on single-factor tests,Box-Behnken design model was applied to investigate effects of ethanol concentration,temperature and ratio of solid to liquid on extraction efficiency. Besides,in vitro antioxidant activities were evaluated simultaneously. Result: Optimum extraction process was as following:extracted 30 min with 42 times the amount of 67% ethanol at 53 ℃;yield of total flavonoids was as high as 12.15 mg·g-1 under these conditions,whose deviation was 1.86% by comparing with the predictive value of 12.38 mg·g-1.Total flavonoids from C. nutans turn out to be equipped with strong reducing power with an C50(the concentration when absorbance was 0.5) of 179.03 mg·L-1,as well as significant scavenging activity to DPPH free radical and ABTS free radical with half inhibitive concentration(IC50) of 89.72,51.92 mg·L-1,respectively.Extract also demonstrated significant protective effect against H2O2-induced hemolysis with an IC50 of 237.22 mg·L-1. Conclusion: Total flavonoids from C. nutans have desired antioxidant activity.关键词：Clinacanthus nutans;total flavonoids;ultrasonic-assisted extraction;antioxidant activity;rutin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize ultrafine grinding technology of Gastrodiae Rhizoma. Method: Taking flour yield,particle size D50,gastrodin content as indexes,single factor tests were adopted to optimize ultrafine grinding technology by taking grinding equipment,particle size of enter shatter,water content,grinding time,medium filling rate and grinding temperature as factors. Result: Bailey grinder was used,particle size of enter shatter selected coarse powder,water content of enter shatter controlled 5%-7%,grinding time was 10-15 min,medium filling rate was 60%-70%,there is no influence to gastrodin content in Gastrodiae Rhizoma when grinding temperature at 0-15 ℃. Conclusion: This study establish some standardized ultrafine grinding process parameters of Rhizoma Gastrodiae,which can provide a reference for application of this herbs.关键词：Gastrodiae Rhizoma;ultrafine grinding;single factor experiment;gastrodin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To analyze reasons why transfer rates of small molecules substances were different through Al2O3 ceramic membrane. Method: Taking four kinds of small molecules effective substances in Huanglian Jiedu decoctions as object of study,computer simulation technology was adopted to analyze interaction between four kinds of effective substances and Al2O3 ceramic membrane,then confirmed by experimental results. Result: Al2O3 ceramic membrane showed some adsorption on berberine hydrochloride,palmatine chloride,baicalin and jasminoidin,transfer rates were 94.21%,90.17%,83.71%,85.61%;adsorption energies were -101.451,-548.142,-797.793,-792.970 kcal·mol-1. Conclusion: Difference of adsorption energies may be main causes of different transfer rates in membrane process.关键词：Huanglian Jiedu decoctions;ceramic membrane;molecular dynamics simulation;interaction;berberine hydrochloride;palmatine chloride;baicalin;jasminoidin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare content differences of geniposide,chlorogenic acid and crocin-Ⅰ from water extracts of different processed products of Gardeniae Fructus for exploring its processing principle. Method: Crude,stir-fired and stir-baked products of Gardeniae Fructus was prepared by standard processing technology in the 2010 edition of‘Chinese Pharmacopoeia’,then extracted by heat reflux water-extraction,HPLC-DAD was employed to determine contents of geniposide,chlorogenic acid and crocin-Ⅰ with detection wavelength of 240,330,440 nm,respectively;mobile phase of methanol-acetonitrile(9: 1,A) and 0.3% formic acid aqueous solution(B) for gradient elution(0-15 min,10%-30%A;15-20 min,30%A;20-60 min,30%-65%A;60-65 min,65%A;65-75 min,65%-10%A) and flow rate of 0.8 mL·min-1. Result: Contents of three ingredients from the crude,stir-fired and stir-baked products were reduced in turn;geniposide content were 2.84%,2.33%,1.57%;chlorogenic acid content were 0.13%,0.09%,0.04%;crocin-Ⅰ content were 0.18%,0.16%,0.11%,respectively. Conclusion: Contents of main medicinal ingredients in water extracts of different processed products exist differences,speculating that it may be processing principle of easing properties of bitter-cold,enhancing cold-blood and hemostatic effect after processing.关键词：Gardeniae Fructus;processed products;geniposide;chlorogenic acid;crocin-Ⅰ
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This study aimed To analyze the volatile constituents of Hydnocarpus anthelminthica to provide the foundation of reasonable utilization of this herb. Method: The volatile oil was extracted from H. anthelminthica by team distillation. The chemical constituents were analyzed by GC-MS. Result: Sixty-three compounds were separated and 49 of them were identified, which accounted for 93.48% of all the volatile oil. The major components were 1,8-cineole (17.28%), trans-anethole (9.28%), 1-isothiocyanato-propane (7.76%), bicyclo[7.1.0]decane (4.73%), peonol (3.25%). Conclusion: The volatile oil of H. anthelminthica was extracted by team distillation and analyzed by GC-MS.关键词：Hydnocarpus anthelminthica;Steam Distillation;GC-MS;volatile oil
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to study onthe determination method of microscopic characteristic constants of pollen grains in Carthami Flos and detect the content of hydroxysafflor yellow A, and analysis of the correlation between the two sideswere analyzed. Method: Capacity analysis was used to quantify pollen grains of Carthami Flos and the content of hydroxysafflor yellow A was determined by HPLC. The data were analyzed by SPSS statistics software. Result: The microscopic characteristic constants of pollen grains and the content of hydroxysafflor yellow A in Carthami Flos were significantly correlated (positive correlation). In other words, results indicated that herbal samples with more pollen grains would contain greater amounts of the pharmacologically active components in Carthami Flos, thus be of better quality in clinical application. Conclusion: It is scientific and feasible to determine the quality of Carthami Flos is by the microscopic quantitative method. The method of based on the microscopic characteristic constants of pollen grains has been preliminarily established. It can be applied to the quality control of Carthami Flos. This approach can be further applied to other medicinal herbs, extending the use of microscopic techniques to the field of quality control of medicinal materials.关键词：Carthami Flos;microscopic quantitative method;pollen grain;hydroxysafflor yellow A;correlation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish a high-performance liquid chromatography(HPLC)method for the determination of both hyodeoxycholic acid and henodeoxycholic acid in the hydrolysate of Fel Suillus simultaneously. Method: The column named Diamonsil C18 (4.6 mm×200 mm, 5 μm) was used for the chromatographic separation. Acetonitrile-0.1%phosphoric acid(45:55)was used as the mobile phase. The flow-rate was set at 1.0 mL·min-1. The detection wavelength was set at 192 nm. The column temperature was maintained at 35 ℃ and the injection volume was 10 μL. Result: Both hyodeoxycholic acid and chenodeoxycholic acid showed good linear relationship in the range of 1.027-8.216 μg and 0.963-7.704 μg, with the correlation coefficients of 0.999 9 and 0.999 0, respectively. The average recovery rates were 99.0% (RSD 1.94%) and 99.5%(RSD 1.76%). Conclusion: The method is accurate, suitable, simple, and can be used for the simultaneous determination of hyodeoxycholic acid and chenodeoxycholic acid in the hydrolysate of Fel Suillus by HPLC.关键词：HPLC;the hydrolysate of Fel Suillus;hyodeoxycholic acid;chenodeoxycholic acid
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The study aimed to determine the contents of the total isoflavones, soy saponins, polysaccharide, protein, and fibrinolytic enzyme of Semen Sojae Praeparatum (SSP)from different regions. And clustering analysis was performed, to provide scientific basis for the study and evaluation of the SSP quality. Method: Genistein was used as a reference to determine the content of total isoflavones using UV spectrophotometry, with measurement wavelength at 260 nm, which showed a good linear relationship in the range of 0.996 4-14.946 mg·L-1. By using vanillin-perchloric acid colorimetric France, oleanolic acid was used as reference to determine the content of soybean saponin with measurement wavelength at 520 nm, and oleanolic acid showed a good linear relationship in the range of 2.2-11 mg·L-1. By using Phenol-sulfuric acid method glucose was used as reference to determine the content of polysaccharides with measurement wavelength at 490 nm, and glucose showed a good linear relationship in the range of 0.002 5-0.02 g·L-1. Determination of content of total protein was performed through Coomassie brilliant blue method with the detection wavelength at 595 nm. The fibrinolytic activity was determined by fibrin plate method, using urokinase as control, which showed a good linear relationship in the range of 100-10 000 U·mL-1. The cluster analysis of five active ingredients in SSP was performed by using SPSS16.0. Result: There was significant difference in the contents of main active components in commercially available SSP from different areas, with the lowest isoflavones content of 0.518 mg·g-1, and the highest of 4.511 mg·g-1; the lowest soybean saponin content was 0.873 mg·g-1, while the highest was 8.623 mg·g-1; the lowest polysaccharide content was 0.28% and the highest was 1.81%; the lowest content of total protein was 4.675%, while the highest was 36.133%. Seven SSP had no plasmin activity, however, among the other SSP with active products, the minimum of plasmin activity was 15.49 U·g-1, and the highest activity was 1 752.08 U·g-1. Cluster analysis showed good correspondence with locations. Conclusion: There are significant differences in the composition of active compounds in SSP from 21 different origins.关键词：Semen Sojae Prapearatum;active component;total isoflavones;soy saponins;polysaccharide;protein;fibrinolytic enzyme
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to study the chemical constituents of essential oils of flower from Gentiana nubigena. Method: The chemical constituents of essential oils were identified and analyzed by GC-MS and area normalization method. Result: Seventy-six compounds were isolated and identified. It included furfural (11.078%), 5-methyl-2-furancarboxaldehyde (7.259%), benzoic acid(6.495%), 2-methoxy-vinyl-phenol (2.120%), palmitic acid(12.623%), (Z,Z)-9, 12-octadecadienoic acid (4.445%), (Z,Z,Z)-9, 12, 15-octadecadienoic acid(3.228%), octadecanoic acid(2.614%),n-tricosane(5.435%)and so on. Conclusion: These compounds were isolated from the flower of the plant for the first time关键词：Gentiana nubigena;volatile oils;GC-MS
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to develop an HPLC method with programmed UV detection wavelength for the simultaneous determination of shanzhiside methyl ester, 8-O-acetyl shanzhiside methyl ester and 2',4'-dihydroxy chalcone in Qizheng Xiaotong plaster. Method: The samples were separated on a Syncronis C18 Dim.(mm) column(4. 6 mm×250 mm, 5 μm)by a linear gradient elution(0-12 min,11% A; 12-17 min, 11%-18% A; 17-25 min, 18% A; 25-40 min, 60% A)using acetonitrile (A) and 0.05% [phosphoric acid solution] (B) as mobile phase at a flow rate of 1.0 mL·min-1 and the eluate was detected by programmed wavelength (0-24 min, 235 nm for shanzhiside methyl ester, 8-O-acetyl shanzhiside methyl ester;24.01-50 min, 365 nm for 2',4'-dihydroxy chalcone ). Result: Shanzhiside methyl ester, 8-O-acetyl shanzhiside methyl ester and 2',4'-dihydroxy chalcone were well separated excellentlyin less than 48 min within the linear range of 15.12-90.72 mg(r=0.999 9), 15.90-95.40 mg(r=0.999 9) and 4.50-27.00 mg (r=0.999 9), respectively. The average recoveries of the compounds listed above were 97.12%, 104.90%, and 105.63%, respectively. Conclusion: Verys Satisfactory and reproducible results arewere obtained for the simultaneous determination of the 3 active compounds in Qizheng Xiaotong plaster.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: A method of high-performance liquid chromatography was developed to evaluate the quality of a traditional Chinese medicine Acorus tatarinowii through analyzing the content of β-asarone and α-asarone. Method: The conditions of sample preparation, chromatography column, detected wavelength and mobile phase were optimized. The linear ranges, precision, reproducibility, stability and accuracy of the method were evaluated based on a Shimadzu HPLC system. And six batches of A. tatarinowii were detected. Result: The determination was performed on a Kromasil C18 column at 257 nm wavelength.The mobile phase was isocratic elution of methanol-water(0.1% formic acid)(70:30). The flow rate was set at 1.0 mL·min-1, and column temperature was at 35 ℃.The regression equation of β-asarone was Y= 2×106X+205.93(r=0.999 9,0.026 96-539 2 μg), and that of α-asarone was Y=6×106X-117.6 (r=0.999 9, 0.016 04-0.320 8 μg); the RSDs of precision were 0.119% (β-asarone) and 0.116% (α-asarone); the RSDs of repeatability were 0.877% (β-asarone) and 0.815% (α-asarone); the RSDs of stability test within 20 hours at room temperature were 0.42% (β-asarone) and 0.25% (α-asarone); the average recovery of β-asarone was 97.7% (RSD 1.9% ), for α-asarone it was 102.3% (RSD 1.5%). The content of β-asarone and α-asarone in A. tatarinowii samples were in the range of 1.12%-3.51% and 0.03%-1.01%, respectively. Conclusion: This method is convenient, accurate and reliable, which can be applied to be a quality evaluation method for A. tatarinowii.关键词：Acorus tatarinowii;β-asarone;α-asarone;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was tTo establish an HPLC method for the determination of cirsimarin in the leaves of Abrus precatorius. Method: HPLC was performed on Hypersil BDS C18 column (4.6 mm×150 mm,5 μm ), and the mobile phase was methanol(A)-0.2% formic acid solution(B) with the gradient elution program(0-25 min, 15%-70% A). The detection wavelength was set at 330 nm, the flow rate was 1.0 mL·min-1. Result: Cirsimarin showed a good linear relationship from 0.327 to 3.27 μg(r=0.999 5), and the regression equation was Y=1933.4X+ 57.897 (r=0.999 5). The average recovery was 99.46% with RSD of 1.78%. Conclusion: The HPLC method is simpley and accurate with agood specificity. It can be used to determine the content of cirsimarin in the leaves of A. precatorius.关键词：Abrus precatorius;HPLC;cirsimarin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This article was aiming to study the volatile components of the compound Jiawei Wendan decoction and to explore its pharmacodynamic material basis of the treatment on autism and related symptoms. Method: The study used HS-SPME, steam distillation(SD) methods combined with GC-MS technique to analyze the volatile components of the compound Jiaweiwendan decoction; the study used HS-SPME-GC-MS to compare the volatile components before and after the decoction. Result: A total of 187 peaks were identified; the highest content was α-asarone, with D-limonene followed. Seventy-four volatile components were detected by SD-GC-MS, among which 66 were identified, accounting for 99.89% of the total volatile components. One hundred and four volatile components were detected before the decoction by HS-SPME-GC-MS, with 85 identified, accounting for 99.79% of the total volatile components. Seventy-seven volatile components were detected after the decoction by HS-SPME-GC-MS, with 65 identified, accounting for 95.45% of the total volatile components. Conclusion: Five volatile components such as α-asarone, D-limonene, L-borneol etc. Paly a role in regulating the central nervous system, improving cerebral function, being anticonvulsant, improving stability and so on, thus can improve the symptoms of autism. The study provided not only a methodological basis for the volatile components Jiawei Wendan decoction, but also a reference to the phamacodynamic material basis of the decoction on the therapy of autism.关键词：Jiawei Wendan decoction;volatile components;autism;HS-SPME;steam distillation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The purpose of the study was to establish a method for the determination of the content of quercetin and total flavonoids in Trichosanthis Pericarpium, and to determine the content of quercetin and total flavonoids in Trichosanthis Pericarpium from different areas. Method: Diverse concentrations of ethanol and methanol were chosen as the extraction solvents for quercetin while it was extracted by immersion extraction, ultrasonic extraction, and refluxing process, then the content was determined by HPLC. The content of total flavonoids was determined using ultraviolet spectrophotometry after it was extracted by ethanol (75%). Result: Refluxing process with methanol proved to be the optimal extraction method for quercetin. The Pericarpium trichosanthis from Jiangsu province showed higher content of quercetin, additionally, the content of total flavonoids in Trichosanthis Pericarpium from Anhui province was much more than other areas. Conclusion: The method established to determine the content of quercetin and total flavonoids in Trichosanthis Pericarpium in this study exhibits good reproducibility and accuracy. The contents of quercetin and total flavonoids in Trichosanthis Pericarpium were significantly different in different areas.关键词：HPLC;UV spectrophotometric method;Trichosanthis Pericarpium;quercetin;rutin;total flavonoids
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This paper mainly studied the application of near infrared spectroscopy with PLS for rapid determination of moisture in Aucklandiae Radix. Method: Through collecting near-infrared spectraby of Aucklandiae Radix by Near-infrared diffuse reflectance spectroscopy technique, with toluene method measurements as a reference value, the quantitative model of moisture was established by partial least squares (PLS), and it was verified by the unknown samples. Result: The correlation coefficients(R2), the root-mean-square error of calibration (RMSEC) and the root-mean-square error of cross-validation (RMSEP) of the quantitative calibration model of moisture were 0.982 9, 0.178 and 0.196, respectively; test value of NIR prediction values and the reference values of the validation set was -0.615 (bilateral P>0.05), implying the difference was not significant. Conclusion: The method is simple, quick, and accurate, and it can be applied to the rapid determination of moisture in Aucklandiae Radix.关键词：near-infrared spectroscopy;partial least squares;Aucklandiae Radix;moisture;rapid determination
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To improve the standard of quality control of Mailuotong Jiaonang. Method: TLC identify Salviae miltiorrhizae radix et rhizoma, Crataegi fructus,Chuanxiong rhizome, Pheretima.The Kromasil 100-5C18(4.6 mm×250 mm, 5 μm) was column adopted with the temperature at 35 ℃, mobile phase A was methanol, mobile phase B was 1% glacial acetic acid solution(0-45 min,25%-45% A; 45-55 min,45%-25%A)the flow rate at 1.0 mL·min-1, the detection wavelength was 254 nm. Result: The calibration curve of puerarin was linear in the range of 0.130 6-1.959 6 μg with the correlation of 0.999 9(n=9). The average recovery of puerarin was 97.77%, RSD 0.70%(n=6). The calibration curve of rutin was linear in the range of 1.054 2-15.813 6 μg with the correlation of 0.999 9(n=9). The average recovery of rutin was 98.66%, RSD 0.89%(n=6). The calibration curve of salvia acid B was linear in the range of 0.184 9-2.773 2 μg with the correlation of 0.999 5(n=9). The average recovery of salvia acid B was 99.55%, RSD 1.15%(n=6). Conclusion: The method is specific, reliable and accurate, so it can be used for the quality control of Mailuotong Jiaonang.关键词：Mailuotong Jiaonang;standard of quality control;puerarin;rutin;salvianolic acid B;TLC;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to isolate and purify the polysaccharide from Plantaginis Semen and to analyse the monosaccharide composition of the polysaccharide. Method: The crude polysaccharide from hot water extraction was subject to remove protein and preliminary isolated through Amberlite FPA90-Cl-+Amberlite IRC-84, purified by DEAE-cellulose chromatography and Sephacryl S-400 chromatography, a polysaccharide fraction named Plantago depressa L. polysaccharide B10(PP-B10) was obtained. The structural characterization and purity of PP-B10 were identified by Ultraviolet-visible Spectrophotometry and fourier transform infrared spectroscopy (FTIR). The monosaccharide composition analyses of PP-B10 was conducted by PMP pre-column derivatization high performance liquid chromatography. Result: A acidic polysaccharide fraction in high purity named PP-B10 was obtained from Plantaginis Semen and it was mainly composed of mannose, ribose, galacturonic acid, galactose, xylose, arabinose and fucose. Conclusion: PP-B10 was a novel polysaccharide with new monosaccharide composition which was found in Plantaginis Semen.关键词：Plantaginis Semen;polysaccharides;monosaccharide composition;pre-column derivatization
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This article aimed to study chemical constituents of Gnaphalium affine. Method: Compounds were obtained from the ethyl acetate extraction of G. affine by silica gel column chromatography and Sephadex LH-20.Their structures were elucidated according to spectral data and physiochemical properties. Result: Thirteen compounds were isolated from G. affine and identified as ethyl caffeate(1), protocatechuic acid(2), E-β-hydroxy-cinnamic acid(3), 4-hydroxyacetophen-one(4), protocatechuic acid ethyl ester(5),3-(4'-formylphenoxy)-4-methoxybenzaldehyde(6), desmethylyangonine-4'-glucopyranoside(7), 4'-hydroxydehydrokawain(8), chlorogenic acid(9), 1,4,5-tri-O-caffeoylquinic acid(10), 1,5-di-O-caffeoylquinic acid(11), 1,5-di-O-caffeoylquinic acid methyl ester(12), and 1,3-di-O-caffeoylquinic acid(13). Conclusion: Compounds 1,2,4-7 and 10 were isolated from Gnaphalium for the first time, and compounds 3 and 8 were isolated from Compositae for the first time.关键词：Gnaphalium affine;chemical constituent;Gnaphalium;structural identification
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study chemical constituents andantibacterial activity of the liposoluble extract from the fruit of Trichilia connaroides var. microcarpa. Method: The liposoluble components was extracted by cold-soak extraction, and their componentswas analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was assayed by paper disk diffusion method. Result: Forty-five peaks were separated and forty-four of them were identified, representing about 92.82% of the total content. The liposoluble extract of the Fruit of T. connaroides var. microcarpa exhibited inhibitoryeffect on Staphylococcus aureus. Conclusion: The liposoluble componentswere extracted for the first timeandthe compoundswere identified. The fatty acids were the main components, accounting for 60.88% of the total liposoluble components. This research could providea scientific basis for further research and development on the fruit of T. connaroides var. microcarpa.关键词：Trichilia connaroides var. microcarpa;liposoluble components;GC-MS;antibacterial activity
- 0
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to study the chemical constitutes of the stem of Myrica nana. Method: Compounds were obtained through reflux extraction with MeOH, column chromatography for separation, and IR, MS and NMR were used for structural identification. Result: Eight compounds were isolated from M. nana, and identified as β-sitosterol (1), taraxerol (2), myricanol (3), kaempferol (4), quercetin (5), myricetin (6), myricitrin (7) and rutin (8), respectively. Conclusion: The chemical constituents of the stem of M. nana were reported for the first time, and compounds 2,4 were obtained from this plant for the first time.关键词：Myrica nana;chemical constituents;myricitrin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This study aimed to conduct a qualitative analysis on chemical components in water extract of Epimedii Folium by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry(HPLC-ESI-Q-TOF-MS). Method: LC-MS-MS was adopted, using C18(4.6 mm×250 mm,5 μm) column with methanol-0.1%formic acid as the mobile phase for gradient elution;ESI ion source was used for mass spectra,and data were collected in positive and negative mode. Result: Ten compounds from water extract of Epimedii Folium were identified by analyzing positive and negative ion mass spectra information and element composition, and comparing controls of data from relevant literatures. Conclusion: After the separation by ultra high performance liquid chromatography,relative molecular mass was determined by mass spectra,and chemical compounds in water extract of Epimedii Folium were determined by information search through relevant literature data,in order to provide powerful therapeutic material basis for water extract of Epimedii Folium.关键词：water extract of Epimedii Folium;chemical components;HPLC-ESI-Q-TOF-MS
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The purpose of this article was to study the chemical constituents of Vitex negundo var. cannabifolia fruits. Method: Constituents were separated and repeatedly purified through column chromatography on silica gel column, ODS and preparative HPLC. Their structures were elucidated by spectral analysis. Result: Eight compounds were obtained from the ethyl acetate extracts, their structures were identified as:casticin (1), detetrahydroconidendrin (2), 6-hydroxy-4β-(4-hydroxy-3-methoxyphenyl)-3α-hydroxymethyl-7-methoxy-3,4-dihydro-2-naphthaldehyde (3), ethyl p-hydroxybenzoate (4), 5,4'-dihydroxy-3,6,7,8,3'-pentamethoxyflavone (5), 5-hydroxy-6,7,3',4'-tetramethoxyflavone (6), 5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone (7), isolariciresinol (8). Conclusion: Compounds 4,5 and 7 were obtained from this genus for the first time. Compound 2 was obtained from this plant for the first time.关键词：Vitex species;Vitex negundo var. cannabifolia fruits;chemical constituents
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to extract, separate and identify the chemical constituents from Hirudo. Method: Compounds were isolated by various chromatographic methods, and their structrues were identified by physicochemical properties and spectroscopic analysis or comparison with reference compounds. Result: From the MeOH extract of the Hirudo, eighteen compounds were isolated and identified as cholesterol (1), hexadecyl ethers of glycerol (2), campesterol (3), nicotinic acid (4), uracil (5), uridine (6), hypoxanthine (7),inosine (8), xanthine (9), phenylalanine (10), propylamine (11), adenosine (12), proline (13), valine (14), L-isoleucine (15), glycerin (16), palmiticacid(17), and succinic acid (18). Conclusion: Compounds 4-6,8-10,12-14,16 and 17 were all obtained from the Hirudo for the first time.关键词：Hirudo;chemical constituents;alkaloid;amino acid
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To prepare dl-tetrahydropalmatine multivesicular liposomes,investigate its in vitro release property and pharmacokinetics in rats. Method: HPLC was adopted to determine the plasma concentration of dl-tetrahydropalmatine with mobile phase of methanol-(KH2PO4-NaOH buffer salts,pH 6.0) (45: 55),flow rate of 0.9 mL·min-1 and detection wavelength at 280 nm.dl-Tetrahydropalmatine multivesicular liposomes were prepared by multiple emulsion method,its shape,particle size and in vitro release characteristics were evaluated.Pharmacokinetics were evaluated in rats following intramuscular injection of dl-tetrahydropalmatine multivesicular liposomes or the reference drug of dl-tetrahydropalmatine injections. Result: Particle sizes of these prepared multivesicular liposomes were even and 80% of them were well-distributed in the range of 15.0-30.0 μm.Encapsulation efficiency was more than 88.47%,in vitro cumulative release was more than 80% in 72 h.Main pharmacokinetic parameters of dl-tetrahydropalmatine injections and multivesicular liposomes were as follows:t1/2 of (3.09±0.37)h and (33.97±4.78)h,Cmax of (289.05±30.37)μg·L-1 and (68.34±8.72)μg·L-1,Tmax of (0.93±0.15)h and (3.92±0.43)h;the same parameter of these two preparation had significant differences by intercomparison. Conclusion: This optimized preparation technology is feasible with good reproducibility,dl-tetrahydropalmatine multivesicular liposomes shows a significant sustained-release property.关键词：dl-tetrahydropalmatine;multivesicular liposome;sustained-release;pharmacokinetics;multiple emulsion method
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The total flavonoids of Broussonetia papyrifera(TFBP ) was investigated for its antitumor activity and induction of apoptosis in vitro, using human HepG-2 hepatocellular carcinoma cell line. Method: The hepatocellular carcinoma HepG-2 cells in logarithmic phase of growth were randomly divided into drug group and control group, with the concentration of 3,6,9,12 g·L-1 TFBP in hepatocellular carcinoma HepG-2 cells intervention durations were 24,48,72 h accordingly. The viability of HepG-2 cells was measured by MTT. Morphology of cell apoptosis was observed by Hoechst 33342 fluorescence staining. The protein expression of B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated X protein(Bax) was analyzed by immunocytochemical method(SP). Result: TFBP inhibited the growth of cells and caused apoptosis significantly. The suppression was both in a time-and dose-dependent manner. When HepG-2 cells were treated with the concentration of 9,12 g·L-1 TFBP in 72 h, the inhibition rate can be achieved 52.46%,64.72% respectively. Compared with control group, there has significant difference (P<0.01). When HepG-2 cells.were treated with the concentration of 9,12 g·L-1 TFBP in 48 h,the typical apoptotic body can be observed under the fluorescence microscope.The increase of caspase-3 activity induced by TFBP showed a concentration-efficiency.TFBP down-regulated the Bcl-2 expression and up-regulated the Bax expression. The ratio of Bcl-2/Bax was evident decrease. Conclusion: TFBP has apparent inhibition and apoptosis-inducing effect on HepG-2 cells. TFBP may induce apoptosis via down-regulating Bcl-2 expression, up-regulating of Bax expression,decreasing the ratio of Bcl-2/Bax and enhancing caspase-3 activities in HepG-2 cells.关键词：total flavonoids of Broussonetia papyrifera;HepG-2 cell line;apoptosis;Bcl-2;Bax;Caspase-3
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To clarify the rationality of traditional Chinese medicine after processing and to provide references for using rational them in the clinic, the pharmacodynamic differences of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma based on the theory of crude and cooked traditional Chinese medicine decoction pieces using in different ways advocated by ZHANG Zhong-jing was investigated respectively. Method: By mice hot-board and acetic-acid-induced twisting experiments, the analgesic effect of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma was observed. By mice auricular swelling model by injecting xylene in the abdomen of mice, the anti-inflammation effect of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma on kunming mouse was observed;by the contractile activity on isolated rat small intestine smooth muscle, the spasmolysis effect of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma on Wistar rat was observed;by turbidimetry method, the anti-platelet aggregation effect induced by ADP or collagen on Wistar rat was observed. Result: Compared to blank group, raw Corydalis Rhizoma and vinegar Corydalis Rhizoma for the analgesic effects caused by the heat conduction and chemical stimulation had obvious difference, and there were also obvious difference between raw Corydalis Rhizoma and vinegar Corydalis Rhizoma(P<0.05,P<0.01). Compared to blank group, raw Corydalis Rhizoma and vinegar Corydalis Rhizoma could inhibit the swelling degrees induced by injecting xylene (P<0.01), but there were no difference between raw Corydalis Rhizoma and vinegar Corydalis Rhizoma;compared to raw Corydalis Rhizoma, vinegar Corydalis Rhizoma could inhibit the contractile activity of isolated rat small intestine smooth muscle (P<0.05,P<0.01). There were no difference between raw Corydalis Rhizoma and vinegar Corydalis Rhizoma on anti-platelet aggregation induced by ADP and collagen. Conclusion: It indicates that Corydalis Rhizoma after processing with vinegar can improve analgesic and spasmolysis effects,corresponding the Chinese medicine processing regulation that the Chinese medicine after processing can increase effects of raw drugs. Using vinegar Corydalis Rhizoma in prescriptions can strengthen analgesic and spasmolysis effects for pain symptoms in the clinic.关键词：Corydalis Rhizoma;pharmacological action;processing
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe protective effect of Modified Sini decoction on immunological liver injury in mice induced by concanavalin A(Con A)and the role of T lymphocyte subsets in which. Method: The mouse were randomly divided into normal saline(NS)group,Con A group,bifendate group(LB,150 mg·kg-1), Modified Sini decoction low,high dosage group(120,240 mg·kg-1).All groups,except for the NS group,were given Con A aqueous solution(18 mg·kg-1)via tail vein injection 1 hour after drug administration at d1 and d3,peripheral blood was collected 12 h after Con A was injected. The percentage of T lymphocyte subsets CD4+,CD8+cells were detected in plasma by a flow cytometer, using enzyme-linked imunosorbent assay(ELISA) for determination the serum cytokines:interferon-γ(IFN-γ),tumor necrosis factor alpha(TNF-α),interleukin(IL)-10 and activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST). Result: The activity of AST and ALT of Modified Sini decoction treatment groups significantly dropped in mice of Con A-mediated liver injury;SND-treated groups could significantly reduced the liver injury model group had elevated inflammatory cytokines IFN-γ or TNF-α level(P<0.05, P<0.01);Modified Sini decoction groups could improve the liver injury model group had lowed T-cell subsets CD4+,CD8+rate(P<0.05). Conclusion: Modified Sini decoction is effective in protecting immunological liver injury in mice induced by Con A and this mechanism maybe closely associated with its action in regulating the T-cell subsets and in reducing the inflammatory cytokines.关键词：Modified Sini decoction;concanavalin-A;immunological liver injury;T lymphocyte subsets
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the mechanism of protection and immune regulation of Wushen Erlian granules in mice with viral myocarditis myocardial. Method: BALB/c mice with viral myocarditis model was established by Coxsackie B3 virus(CoxB3 ).The BALB/c mices were randomized into the normal group,model group, positive control ribavirin group(1 mg·kg-1)and Wushen Erlian granule low-dose group, middle-dose group, high-dose group (4,12,20 mg·kg-1). After modeling, corresponding drugs were respectively given to each mice in all groups at d0, d1,d2,d3,d4,d5 for 5 days. Weighing and specimen collection according to the testing requirements were carried out. Myocardial enzyme indexes detection, viscera index detection and natural killer cells(NK)killing experiment were performed. Result: After second days, micein model group appeared apathetic, reduced action, rough, hypothermia. After fifth days, mice were killed and the heart was dark, texture was not clear, the heart surface appeared white spot funicular lesions. Pathological HE staining indicated inflammatory cell and necrotic lesionsof myocardial tissue. At the same time, Compared with normal group, creatine kinase isoenzyme, troponin and myoglobin of model animals were increased significantly (P<0.05), which showed the model was effective. Compared with model group, creatine kinase isoenzyme and troponin in ribavirin group, high dose group and middle dose group of Wushen Erlian granule were declined, with statistically significant difference(P<0.05). Spleen index and thymus index in ribavirin group, high dose group and middle dose group of Wushen Erlian granule were increased (P<0.05). The test result of killing effect of NK showed ribavirin, high dose and middle dose of Wushen Erlian granule group compared with model group and normal group were significant different(P<0.05). Conclusion: Wushen Erlian granules can alleviate myocardial injury caused by CoxB3 and stimulate immune regulation mechanism of the body, with has certain protective effect on myocardial cells.关键词：Wushen Erlian granules;viral myocarditis;immunomodulatory
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate effect and mechanism of new Weinaian preparation (NWP) on the rat chronic superfical gastritis(CSG) induced by iodoacetamide. Method: SD rats were randomly divided into normal control group(NC, n=10), CSG group(n=15),NWP(9.10, 2.87,0.91 g·kg-1) groups(n=15, respectively)and Weinaian capsule group (n=15). The CSG rats were induced by 0.1% iodoacetamide for 3 weeks, the drug groups were given by gavage once a day for 3 weeks with appropriate drugs simultaneously, and the nomal contral and CSG groups were given the same amount of water.The effects of NWP on the CSG rats were evaluated by naked eye, pathological section and transmission electron microscope. The action mechanism of Weinaian was studied by detection the level of malondialdehyde(MDA) and superoxide dismutase(SOD) in the rat gastric mucosal. Result: Contrasted with the NC group,there was obvious injury and inflammation cell in the gastric mucosa of the CSG group. The effects of anti-injury were found in the ranitidine and high-dose group of Weinaian groups (P<0.01), there was dose-reponse relationship in the three Weinaian groups. The ultrastructure injury of chief and parietal cell were improved by the high-dose group of Weinaian.The group also reduced the level of MDA and increased the activity of SOD in the gastric mucosa(P<0.01, vs CSG group). Conclusion: The new Weinaian preparation can alleviate the gastric mucosal injury of CSG rat induced by iodoacetamide which maybe related to clearing gasrica mucosa radical.关键词：Weinaian;iodoacetamide;chronic superfical gastritis;radical
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the antidepressant-like activity of extracts from Pericarpium citri, and its mechanism related to brain derived neurotrophic factor (BDNF) in mice exposed to chronic mild unpredictable stress (CUMS). Method: Male ICR mice were randomly divided into 6 groups:control group, CUMS group(20 mg·kg-1), fluoxetine group and P. citri groups (100, 200, 400 mg·kg-1). The treatment groups, except control group, were exposed to CUMS for 8 weeks and administrated for 4 weeks. Sucrose preference test, novelty suppressed feeding test and forced swimming test were used to evaluate the antidepressant-like effect of extracts from P. citri. BDNF expressions were measured by qPCR and ELISA. Result: CUMS induced reduction of sucrose preference, increase of latency to feed and immobility time, decrease of hippocampal BDNF expressions. Compared with CUMS mice, extracts of P. citri significantly increased sucrose preference, decreased latency to feed and immobility time. In addition, P. citri elevated the expression of BDNF in hippocampus. Conclusion: Our results suggest that extracts from P. citri exhibit antidepressant-like activity, which may be mediated by elevation of hippocampal BDNF.关键词：Pericarpium citri;antidepressant;chronic mild unpredictable stress;brain derived neurotrophic factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate effects of Dendrobium nobile Lindl. alkaloids (DNLA) on blood glucose and gene expression of insulin receptor substrate 2(IRS-2) and insulin-like growth factor 1(IGF-1) in liver of rats with diabetes. Method: Male Spragye-Dawley rats were fed with high-fat diet for 6 weeks. Then streptozotocin(STZ) 40 mg·kg-1 was injected intraperitoneally once to induce diabetic rat model. Diabetic rats were randomly divided into model group, metformin (metformin 100 mg·kg-1) and DNLA 20, 40, 80 mg·kg-1 group. And normal control group was fed with basal feed. After intragastric administration of metformin, DNLA and isopyknic distilled water daily for 4 weeks to rats of metformin group, DNLA group and normal control group respectively, the fasting plasma glucose (FPG) and fasting insulin (FINS) were determined, and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. The expressions of IRS-2 mRNA and IGF-1 mRNA in liver were determined by real time RT-PCR. Result: Compared with the normal control group, the levels of FPG, FINS, HOMA-IR were significantly increased (P<0.05). The FPG, FINS and HOMA-IR were significantly decreased in the metformin group and DNLA 40, 80 mg·kg-1 group compared with those in the model group(P<0.05). Contents of IRS-2 mRNA and IGF-1 mRNA in model group were decreased; compared with model group, contents of IRS-2 mRNA and IGF-1 mRNA in the metformin group and DNLA 40, 80 mg·kg-1 group were increased. Conclusion: DNLA can significantly decrease the levels of FPG of diabetic rats, and its mechanism may be related with enhancing the expressional levels of IRS-2 mRNA and IGF-1 mRNA in liver, improvement of insulin resistance.关键词：Dendrobium loodigesii;alkaloids;diabetes mellitus;insulin receptor substrate 2;insulin-like growth factor 1
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the curative effect and mechanism of the water extract from Siraitiae Fructus on acute pharyngitis model rats. Method: For 5 consecutive days,the ammonia saray was used to establish the animal model of acute pharyngitis. the modeling rats were randomly divided into blank group, dexamethasone group, the water extract of Siraitiae Fructus groups (0.5,1.5,4.5 g·kg-1), and lavage administration for 5 days.The pharyngeal pathological changed, white blood cell count (WBC) and the expression of interleukin (IL)-1β,IL-6 and tumor necrosis factor alpha(TNF )-α in serum were observed. Result: The middle and high dose of the water extract of Siraitiae Fructus could improve the pathological changes (P<0.01) and WBC (P<0.05,P<0.01). It had obvious inhibitory effect on the expression of IL-1β(P<0.05,P<0.01),IL-6 (P<0.01) and TNF-α (P<0.01) in serum. Conclusion: The water extract of Siraitiae Fructus has good treatment effect on the acute pharyngitis, its mechanism may be related to inhibition of pharyngeal pathological changes and the expression of inflammatory cytokine.关键词：Siraitiae Fructus;acute pharyngitis;animal model;pharmacological effects;inflammatory cytokine
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effects of Buyang Huanwu decoction compound granules(BYHWDCG) on the plasma dynorphin(Dyn) and neuropeptide Y(NPY)contents in ischemic cerebral stroke rats. Method: The model was reproduced by the four-vessel occlusion (4-VO) method with some modifications.The rats were treated with BYHWDCG (30.4,15.2 g·kg-1). The Dyn and NPY levels were assayed by ELISA. Result: The plasma Dyn and NPY levels in model group were increased compared with control group. However, after treated with BYHWDCG, the contents of Dyn and NPY in plasma significantly decreased. Conclusion: The BYHWDCG can protect rats with the ischemic stroke by down-regulation of neurotrophic factors.关键词：Buyang Huanwu decoction compound granules;ischemic cerebral stroke;dynorphin;neuropeptide Y
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare the efficacy of synthetic borneol and S101 of rabbit blood pressure, heart rate and to study whether S101 can replace synthetic borneol. and provide a new medicinal resource of borneol on clinical. Method: Using RM6240 biological signal acquisition system, in which the condition is not modeling observe within 1-5 mg·kg-1 dose, diastolic blood pressure, the impact of the two drugs on systolic heart rate in normal rabbits. Result: ①Within the dose of 1-5 mg·kg-1, the efficacy of the two drugs has significantly positive correlation with dose.②At the dose of 5 mg·kg-1, synthetic borneol group and S101 group decrease systolic, diastolic blood pressure and heart rate than control group (P<0.01).S101 has lowering effect than synthetic borneol. (P<0.05, P<0.01). Conclusion: Both synthetic borneol and S101 can reduce systolic and diastolic blood pressure. slow heart rate. The effect of S101 is better than synthetic borneol. S101 has the significance of alternative synthetic borneol.关键词：synthetic borneol;S101;blood pressure;heart rate
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effects of the anti-inflammation and analgesia and the change of the serum 5-hydroxytryptophan (5-HTP)and 5-hydroxyindole acetic acid (5-HIAA) by Duhuo Jisheng decoction on adjuvant arthritis rats. Method: The experiment set up blank group, model group, positive group, low, middle and high dose groups of Duhuo Jisheng decoction. Except the blank group, other groups were injected Complete Freund's adjuvant (FCA). In the administration for 14 days, the blank group and model group were perfused with carboxymethyl cellulose sodium(CMC-Na),Duhuo Jisheng decoction low, middle and high dose groups were fed with Duhuo Jisheng decoction 0.85,1.7,3.4 g·kg-1 and the positive group were given aspirin 0.20 g·kg-1. Determination of serum 5-HTP and 5-HIAA by ELISA method. Result: Duhuo Jisheng decoction can significantly increase the thymus weight, and can obviously reduce the content of the serum 5-HTP and 5-HIAA compared with the model group (P<0.05). Conclusion: Duhuo Jisheng decoction has a good anti-inflammatory and analgesic function, and the mechanism of Duhuo Jisheng decoction curing the adjuvant arthritis may relate with its reducing the content of the serum 5-HTP and 5-HIAA.关键词：Duhuo Jisheng decoction;adjuvant arthritis;5-hydroxytryptophan;5-hydroxyindole acetic acid
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to observe the effects of pomegranate ellagic polyphenol (PEP)on pacreatic islet function of diabetes mice. Method: Streptozotocin was intraperitoneally injected to induce diabetes in mice, and the diabetes mice were randomly divided into model group, low-dose PEP group, high-dose PEP group and glibenclamide group,while normal mice were taken as control group. PEP was intragastricly administrated for 30 d,body weight, fasting blood glucose,sugar tolerance were tested, HE and immunohistochemistry pancreas slice were made to observe pathohistological changes and expression of transcription factor pancreatic duodenal homeobox-1(PDX-1). Result: Compared with diabetes mice, in mice with PEP administration, the body weight, pacreatic islet area and PDX-1 expression increased, while the fasting blood glucose decreased(P<0.05). Conclusion: PEP has a certain degree of effect on the improvement of pacreatic islet function in diabetes mice,and the machanism might be involved in upregulating the expression of protein PDX-1.关键词：pomegranate ellagic polyphenol;diabetes;islet function;pancreatic duodenal homeobox-1
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to study the effect of Fufang Xueshuantong on albumin-creatinine ratio(ACR), liver function and lipid metabolism of patients with early-stage diabetic nephropathy. Method: Patients with early stage diabetic nephropathy in our hospital from 2011 June to 2013 July were enrolled and given support treatment and hypoglycemic therapy. Then patients were divided into observation group given enalapril combined with fufangxueshuantong and control group only given enalapril. ACR, blood urea nitrogen (BUN), creatinine clearance rate (CCr), aspertate aminotransferase (AST) and alanine aminotransferase (ALT), triglycerides (TG), cholesterol (TC), low density lipoprotein (LDL), high-density lipoprotein (HDL) between two groups were compared. Result: In observation group, the ACR,urine protein, BUN, CCr, ALT, AST,TC,TG,LDL (4.5±2.4)mg·g-1,(0.12±0.06)mg/24 h,(5.8±1.4)mmol·L-1,(86.4± 15.6)μmol·L-1,(37.8±7.2),(56.4±7.5)U·L-1,(2.9±0.3),(1.1±0.2),(2.1±0.3)mmol·L-1 were significantly lower than control group(6.6±2.3)mg·g-1,(0.19±0.07)mg/24 h,(6.5±1.3)mmol·L-1,(97.8±22.3)μmol·L-1,(53.5±10.9),(50.9±7.4)U·L-1,(5.7±0.7),(1.8±0.3),(3.8±0.5)mmol·L-1;HDL(1.3±0.2)mmol·L-1was significantly higher than control group(0.7±0.1)mmol·L-1 (P<0.05 or P<0.01). Conclusion: Fufang Xueshuantong is an ideal therapy in treating early-stage diabetic nephropathy for it can improve renal function, liver function and lipid metabolism.关键词：diabetic nephropathy;Fufang Xueshuantong;liver function;lipid metabolism
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This study aimed to observe the clinical efficacy and safety of Bushen Huoxue Kaiqiao fang in the secondary prevention of the diabetes-induced vascular mild cognitive impairment (kidney deficiency and blood stasis). Method: Eighty-five cases of diabetes-induced vascular mild cognitive impairment were randomly divided into treatment group (43 cases) and control group (42 cases). The treatment group received the Bushen Huoxue Kaiqiao fang treatment three times a day orally and an oral administration of aspirin 100 mg once daily, while the control group were given oral administration of aspirin 100 mg once daily alone. The treatment continued for six months, every 6 months after the treatment there was a follow-up study till 12 months after the treatment.Daily living scale (ADL), Montreal Cognitive Assessment Beijing Edition (MoCA), P300 and traditional Chinese medicine(TCM) symptom scores were used for evaluation. Result: The variance analysis of repeated measurements analysis of variance revealed that, in both groups, the ADL scale, MoCA scale, P300 and TCM symptom score after treatment were all better than those before treatment at different periods, with the differencesstatistically significant (P<0.05). However, as for the comparison between treatment group and control group, there was no statistically significant difference in both sharply after the treatment and 6 months after the treatment, by t test of ADL scale, MoCA scale, P300 and TCM symptom rating scale scores;while the ADL scale, MoCA scale, P300 and TCM symptom rating scale scores of treatment group all showed statistically significant difference (P<0.01) between sharply after the treatment and 12 months after the treatment, compared with the control group. The incidence of adverse events in both groups showed no statistical significance by χ2 test. Conclusion: Bushen Huoxue Kaiqiao fang can only effectively prevent diabetes-induced vascular cognitive dysfunction continuing to develop, delaying its natural course of the disease, and can improve the patient's activities of daily living, cognitive function and clinical symptoms.关键词：Bushen Huoxue Kaiqiao fang;diabetes-induced vascular mild cognitive impairment;secondary prevention;aspirin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This study aimed to observe the effect of Jiangtang Baoxin pill on the inflammatory factors and endothelial function in patients with type 2 diabetes(T2DM)and unstable angina pectoris(UAP). Method: Seventy-eight patients with T2DM and UAP were randomly divided into two groups:the control group (40 cases) was given conventional therapy;the observation group(38 cases) was given Jiangtang Baoxin pill(6 grams once,three times daily)in addition to conventional treatment. These patients of two groups were treated for 6 months. At the same time, 30 healthy people were selected as the healthy control group. Lipids,inflammation and endothelial function indicators were detected before and after treatment,clinical efficacy in patients of two groups were observed before and after 6 months of treatment,cardiovascular events were followed up for 6 months. Result: After 6 months of treatment,the total efficacy of the observation group was significantly higher than that of the control group(P<0.05);the levels ofinterleukin-6(IL-6), tumor necrosis factor-α(TNF-α), matrix metalloproteinase 9(MMP-9), high-sensitivity C-reactive protein(hs-CRP)of patients in the observation group were significantly lower than those in the control group(all P<0.01);the improvement of low-density lipoprotein-cholesterol(LDL-C),triacylglycerol(TG),nitric oxide(NO),endothelin-1(ET-1) in the observation group were significantly better than those of the control group(all P<0.01);Incidence of cardiovascular events of the observation group was significantly lower than that of the control group within 6 months(P<0.05). There was no adverse reaction associated with Jiangtang Baoxin pill in the observation group within 6 months. Conclusion: It was safe and effective that Jiangtang Baoxin pill can be used in the treatment of patients with T2DM and UAP,in which the antagonism of inflammatory reaction and improvement of endothelial function may be one of the mechanisms.关键词：type 2 diabetes;unstable angina pectoris;inflammatory factor;endothelin;Jiangtang Baoxin pill
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of oral Kangmin decoction and acu-point application of traditional Chinese medicine on children with allergic rhinitis (AR), and the influence on the levels of total immunoglobulinE (IgE), interleukin-4 (IL-4), interleukin-10 and (IL-10). Method: One hundred and sixteen patients with AR were divided into control group and observation group of 58 patients each, and 25 healthy subjects were assigned to a healthy control group. Patients in control group took oral 5-10 mg levocetirizine dihydrochloride tablets once daily for 4 weeks, and took budesonide nasal spray for 10 days when needed. Based on the treatment of control group, patients in observation group received oral Kangmin decoction one dose daily plus acu-point application of traditional Chinese medicine once weekly for 4 weeks.Rhinitis symptoms score was conducted before and after treatment, and attack rate of AR were recorded before and one week after treatment. Quality of life were assessed and the levels of total IgE, IL-4, IL-10 were detected before and after treatment. Result: The total clinical effective rate of 100% in observation group was better than 86.2% in control group (P<0.05). Rhinitis symptom score, AR attack rate and quality of life score after treatment in observation group were lower than those in control group (P<0.01). Blood levels of total IgE and IL-4 in patients with AR in two groups were higher, and IL-10 level was lower than those in healthy control group before and after treatment (P<0.01). The levels of total IgE, IL-4 in two groups decreased, IL-10 increased after treatment, as compared with that before treatment (P<0.01). While levels of total IgE and IL-4 were lower, IL-10 level was higher in observation group than those in control group (P<0.01). Conclusion: Based on the conventional western medicine therapy, oral Kangmin decoction and acu-point application of traditional Chinese medicine have significant short-term curative effect in treating AR, which may be achieved by reducing level of IgE and regulating balance of Thl/Th2.关键词：allergic rhinitis;Kangmin decoction;acu-point application;immunoglobulin E;interleukin-4;interleukin-10
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of the extract of ginkgo biloba leaves injection (EGBLI) on microcirculation and hemorheology in patients with lung cancer undergoing chemotherapy. Method: One hundred patients with lung cancer undergoing chemotherapy were divided into EGBLI group and heparin sodium injection (HIS) group of 50 patients each by a stratified random sampling method, and 25 healthy subjects were assigned to a healthy control group. Patients with lung cancer received first-line chemotherapy. Patients in HIS group received 70-80 units/kg/day heparin sodium injection (iv drip, twice daily). Patients in EGBLI group received 20 mL extract of ginkgo biloba leaves injection (iv drip, once daily). The patients received two 10-day periods of treatment separated by 2 days without treatment. Plasma fibrinogen (Fib), D-dimer (D-D), platelets (PLT) and maximum platelet aggregation rate (MPAR) and hemodynamic indexes were detected before and after treatment. Result: The levels of Fib, D-D and PLT in lung cancer patients were obviously higher than those in healthy subjects (P<0.01). The levels of Fib, D-D,MPV and MPAR after treatment in EGBLI group were lower than those in HIS group (P<0.01). The better improvement of blood viscosity (high shear,low shear), whole blood reductive viscosity (high shear, low shear), plasma viscosity, hematokrit and erythrocyte sedimentation rate were found in EGBLI group as comparied with in HIS group (P<0.01). No significant impact on effective rate of gender and pathological factors were found. Significant higer clinical effective rate were found in patients with tumor metastasis than that with no umor metastasis (P<0.05). The cumulative adverse reactions rate in EGBLI group was 12%, which was lower than 40% in HIS group (P<0.01). Conclusion: There was significant improvement of hypercoagulable state in lung cancer patients by extract of ginkgo biloba leaves injection. Besides, it is better and safe for lung cancer patient with no tumor metastatic.关键词：lung cancer;chemotherapy;extract of ginkgo biloba leaves injection;fibrinogen;D-dimer;blood platelet
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To discuss the short-term and long-term effects of oral traditional Chinese medicine combined with retention enema of Qingkui decoction in treating patients with ulcerative colitis (UC). Method: One hundred and sixty patients with UC were randomly divided into control group and observation group of 80 patients each. Patients in control group received oral sulfasalazine tablets plus prednisone for 8 weeks, then received maintenance treatment with sulfasalazine tablets for 2 months for patients in remission. Patients in observation group received oral traditional Chinese medicine combined with retention enema of Qingkui decoction for 8 weeks, then received maintenance treatment with corresponding Chinese patent medicines according to differentiated syndrome for 8 weeks for patients in remission. And patients in remission received a 12 month follow-up. Before and after treatment, colonoscopy was performed and the results were evaluated by Baron scoring method. The disease activity was scored by Sutherland DAI. Quality of life in inflammatory bowel disease (IBD) was assessed by IBD questionnaire (IBDQ). The main signs and symptoms, were scored, and the recurrence rate of patients was recorded. Result: The total effective rate in observation group was 91.2% superior to 75% in control group(P<0.05). Baron score and Sutherland DAI score in observation group were lower than those in control group (P<0.01). The score of diarrhea, bloody purulent stool and stomachache in observation group were lower than those in control group (P<0.01). IBDQ scores including intestinal tract symptom, constitutional symptom, emotional capability and social competence in observation group were higher than those in control group (P<0.01). Recurrence rate in observation group within 12 months was (0.76±0.41) less than (1.28±0.64) in control group (P<0.01). Conclusion: Induction and maintenance treatment of oral Chinese patent medicine combined with retention enema of Qingkui decoction is an effective therapy to prevent the activity of UC, improve the healing of anabrosis, control clinical symptoms and reduce recurrence rate.关键词：ulcerative colitis;Qingkui decoction;retention enema;induction and maintenance treatment;quality of life;recidivation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Yishen Jianpi decoction on renal function and bone metabolism in patients with chronic kidney diseases stages 3 and 4 (CKD stages 3 and 4) combined with deficiency of spleen and kidney in Chiese medicial syndrome. Method: Eighty patients were randomly divided into control group and Yishen Jianpi group of 40 patients each. Patients in control group received 0.25 μg of calcitriol pills plus 0.16 g of vitamin D2 calcium lactate tablets po. Once daily for a total of 3 months as conventional western medicine treatment. Based on the conventional western medicine treatment, patients in Yishen Jianpi group received a modified Yishen Jianpi decoction one dose daily. Blood urea nitrogen(BUN), serum creatinine(SCr) and 24-hours urine protein ration(24 h) were detected before and after 3 month treatment. Glomerular filtration rate (eGFR) was calculated. Deficiency syndrome of spleen and kidney was graded every month. Levels of intact parathyroid hormone (iPTH), blood calcium, serous phosphorus (P), bone gla protein (BGP), bone alkaline phosphatase (BAP) and leptin (LP) were detected.Before and after treatment. Result: The total clinical effective rate of 52.5% in Yishen Jianpi group was better than 30% in control group with a significant difference between the two groups(P<0.05). The levels of BUN, SCr and 24 h Upr after treatment in Yishen Jianpi group had a decrease than before treatment (P<0.05), and the SCr in Yishen Jianpi group were lower than those in control group(P<0.05). The spleen and kidney deficiency scores in Yishen Jianpi group were significantly lower as compared with those after two or three month treatment in control group (P<0.05 or P<0.01). The level of serum Ca in Yishen Jianpi group were higher (P<0.05) and iPTH, serum P, BGP in Yishen Jianpi group were lower after treatment than those in control group (P<0.05). The levels of serum BAP and LP had a decrease in two groups than before (P<0.01), which was lower in Yishen Jianpi group than in control group (P<0.01). Conclusion: Yishen Jianpi decoction can improve the renal function of patients with CKD stages 3 and 4 Meanwhile, Yishen Jianpi decoction exerts certain adjustment to bone metabolic disorders.关键词：chronic kidney diseases;bone metabolic abnormalities;Yishen Jianpi decoction;bone alkaline phosphatase;leptin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To discuss the influence of Fuzheng Huadu decoction on hypercoagulable state by assessing blood coagulation factor, platelet activity, blood fat and hemorheology in patients with diabetic kidney disease(DKD) undergoing maintenance hemodialysis. Method: Ninety-six patients with DKD and uremic were randomly divided into control group and observation group of 48 patients each. All patients received basic therapy of conventional and supportive treatment. Patients in control group took oral 5 g Niaodu Qing granules 4 times daily. Patients in observation group took oral Fuzheng Huadu decoction 1 dose daily. All patients in two groups received one 4 month periods of treatment. The levels of fibrinogen(FIB), maximum aggregation rate (MPAR), D-dimer (D-D), von willebrand factor (vWF), thromboxane B2 (TXB2), 6-Ketone-prostaglandin F1a (6-Keto-PGF1a) and platelet α-glair of membrane particle (GMP-140) were detected. Also, hemorheology indexes and levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol(LDL-C) were detected before and after treatment.Result: After treatment, the level of 6-keto-PGF1a in observation group was higher than that in control group, and levels of TXB2, MPAR and GMP-140 were lower than those in control group (P<0.01). The levels of D-D, FIB and vWF were lower than those in control group (P<0.01). Ameliorate of whole blood viscosity (high and low shear), plasma viscosity, hematokrit and erythrocyte sedimentation rate were superior to those in control group (P<0.01). The levels of TC, TG and LDL-C in observation group were lower, and the level of HDL-C was higher than in control group (P<0.01). Conclusion: Fuzheng Huadu decoction can improve hypercoagulable state in patients with DKD undergoing maintenance hemodialysis. This effect is likely mediated by reducing promote clotting factor, inhibiting platelet activity, regulating lipid metabolism, preventing thrombus formation and improving microcirculation in patients.关键词：diabetic kidney disease;Uremia;hypercoagulable state;Fuzheng Huadu decoction
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 -
Clinical Observation of Ershi Wuwei Shanhu Pills Combined with Acupuncture on Migraine with 55 Cases
摘要：Objective: Clinical curative efficacy and mechanism of action of Ershi Wuwei Shanhu pills combined with acupuncture in treating migraine were investigated. Method: One hundred and ten patients with migraine were randomly divided into control group and observation group (55 cases,respectively) by random number table. Patients in control group received treatment of acupuncture, and patients in observation group received Ershi Wuwei Shanhu pills and acupuncture. Course of treatment in two groups were both 5 weeks. Frequency of headache attack, degree of headache, days of headache and accompanied symptoms were observed before and after treatment. Visual analogue scales were recorded before treatment and at the 2, 12, 24, 36, 48, 60, 72 h after treatment. Cerebral blood flow velocity was detected by transcranial Doppler (TCD) before and after treatment. And levels of β-endorphin (β-EP), endothelin(ET), 5-hydroxytryptamine(5-HT) and nitric oxide(NO) in serum were detected. Result: VAS scores in two groups decreased significantly at the 2nd hour after treatment(P<0.01),and there was a decline of VAS scores in two groups at the 12, 24, 36, 48, 60, 72 h after treatment. VAS score in observation group was lower than in control group at the 72 nd hour after treatment (P<0.05).Scores of times of headache attack, degree of headache, days of headache and accompanied symptoms of headache and total scores in observation group were all lower than those in control group(P<0.01).Coronary flow velocity of middle cerebral artery(MCA), anterior cerebral artery(ACA), posterior cerebral artery(PCA), vertebral artery(VA) and basilar artery(BA) were all lower than those in control group(P<0.01). Levels of β-EP, NO and 5-HT in observation group were higher than those in control group and level of ET was lower than in control group(P<0.01).The total effective rate in observation group was 94.5%, which is superior to that in control group 84%(P<0.05). Conclusion: Ershi Wuwei Shanhu pills combined with acupuncture had obvious curative efficacy in treating migraine, the mechanism may be related to improving levels of β-EP, NO and 5-HT, reducing level of ET, and ameliorating brains'blood flow velocity.关键词：migraine;Ershi Wuwei Shanhu pills;acupuncture and moxibustion;clinical evaluation- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This investigation aimed at applying data mining technology to explore the relationship among symptoms,patterns and medication regularity of hypertension. Method: All the relative literatures were collected from sinomed database,then subject to data slicing algorithm to mine out the relationship among symptoms, patterns and medication regularity of hypertension. Result: The most common symptoms of hypertension are headache(2 650), dizziness(1 734),and the patterns are manifested as liver Yang hyperactivity, Yin deficiency and Yang excess as well as upward liver fire, the most injured organ are liver and kidney.Treating methods would be to suppress the upward liver yang and strengthen liver and kidney which should be accompanied by activating Qi and Blood.The most common applied herbs are Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis. Conclusion: Text mining is a branch of data mining and a practicable technology which can be used in the field of traditional Chinese medicine(TCM).Through putting this technology in the research of TCM clinical practice,both doctors and patients could benefit from it.关键词：text mining;data slicing algorithm;hypertension;medication regularity
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of the study was to evaluate the effectiveness of Xipayi Mouth Rinse in the treatment of simple gingivitis. Method: We systemically searched China National Knowledge Infrastructure (CNKI)(1979—2012), Weipu Database for Chinese Technical Periodicals (1989—2013), PubMed and Cochrane Library.The methodological quality of the included trials was assessed based on the Jadad scale and the available data were analyzed with RevMan software (version 5.2.0). Result: A total of 6 studies involving 490 participants satisfied the minimum criteria for Meta-analysis.The evidence showed that the Xipayi Mouth Rinse was compared with placebo for treatment of simple gingivitis sulcus bleeding index(SBI)[WMD=-0.59,95%CI(-0.76,-0.42)], plaque index(PLI)[WMD=-0.67,95%CI(-1.03,-0.30)];the Xipayi Mouth Rinse was compared with western medicine for treatment of simple gingivitis [WMD=-0.02,95%CI(-0.14,0.10)],PLI[WMD=0.03,95%CI(-0.24,0.30)]. Conclusion: The current clinical evidence showed that the effectiveness of Xipayi Mouth Rinse in the treatment of simple gingivitis higher than the Placebo, similar effects with western medicine. Bedides,it can reduce the sulcus bleeding index and plaque index with no significant side effects, and it is easy to take. However, due to the low quality studies and small sample, it is still need to be confirmed by the high-quality, multi-center, large sample, randomized, double-blind clinical controlled trials.关键词：Xipayi Mouth Rinse;gingivitis;systematic review;Meta-analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Many achievements in the mechanisms of compatibility of traditional Chinese medicine (TCM) based on the effect of drug metabolism have been procured by new technologies in drug metabolism and metabonomics in recent years. Interaction of drug metabolism was one of the mechanisms of decreasing toxicity and increasing efficacy on the compatibility of TCM. The behaviors of active compounds of TCM in vivo, such as absorption, distribution, metabolism and excretion probably changed significantly after compatibility, therefore its toxicity and activity changed. This paper summarized the studies on compatibility of TCM based on the effect of drug metabolism for the first time.关键词：drug metabolism;metabonomics;compatibility of traditional chinese medicine
- 5
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：The quality control of Chinese herbs plays important roles in clinical application and practice. At present, it is insufficient to indicate the quality control of Chinese herbs by determination of single-component or a few components. Therefore, improving the standard of quality control of Chinese herbs is of great significance to evaluation, planting and deep processing of Chinese herbs. Based on the current approach of quality control of Chinese herbal medicine, we put forward the research idea that combines the spectrum effect relationships with the multi-components quantities by one marker to improve overall quality control standard of Chinese herbal medicine. First of all, we must elucidate the chemical composition that is related to curative effects through spectrum. Second, their structures were determined by HPLC, LC-ESI-MSn, and column chromatograph. Finally, the quality of medicinal materials was appraised through the multi-components quantities by one marker. It has been used to provide a research method of quality control of medicinal materials and promote internationalization of Chinese herbal medicine.关键词：Chinese herbs medicine;quality control standards;fingerprint;spectrum effect relationships;multi-components quantities by one marker
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：The aim of this study was to summarize the processing induced osteogenic differentiation and modern applications of bone marrow mesenchymal stem cells so as to provide a reference for its further study.By referring to the relevant literatures,the processing research of bone marrow mesenchymal stem cells was summarized and analyzed.The review mainly focused on the biological characteristics of bone marrow mesenchymal stem cells,induced osteogenic differentiation, the application of tissue engineering bone,the problems of bone marrow mesenchymal stem cells at present and the research direction in the future. It has been revealed that bone marrow mesenchymal stem cells have the multi-potential of differentiation and can be induced to differentiate into osteogenic ex vivo. Bone marrow mesenchymal stem cells can be used for wound healing, cell replacement therapy, hematopoiesis and gene therapy due to their characteristics. Understanding the mechanisms of induction and differentiation will be of important value to the research method of differentiation of bone marrow stromal cells into osteoblasts.关键词：bone mesenchymal stem cells;osteoblast cells;osteogenic differentiation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：The related issues on the development of Chinese innovative drugs were mainly discussed in this paper. The author argued that 1) the breakthrough point of Chinese innovative drugs should focused on the Chinese clinical practice;2) the combination of component or composition derived from Chinese medicine may be an important research direction but can not substitute the compatibility of Chinese pieces;3) the research on the component combination under the guidance of the traditional Chinese medical theory can apply to medical therapy following syndrome differentiation, whose purpose is to enhance drug safety,clinical suitability,and to use drugs with clear ingredients and action mechanisms,for more stable and controllable product quality for commercial production.So the way the component combination founctions still relies on the multi-components, multi-targets and multiplex regulation.Chinese medicine should not blindly pursue and meet the international standards but to establish its own characteristicquality standards. The serve target of Chinese innovative drugs should focus on Chinese people.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：To provide reference for the clinical application and rational development of Hedyotis diffusa, according to its recent development of research on the chemical constituents and pharmacological actions. Data of the chemical constituents and pharmacological actions of Hedyotis diffusa were collected, summarized and analyzed in domestic and foreign database such as CNKI, Wanfangdate, PubMed, SpringerLink, systematically. Terpenoids, flavonoids, anthraquinones, sterols, etc, were the main chemical composition in Hedyotis diffusa. Anticancer, antioxidant and anti-inflammatory effect were the main pharmacological effects of this drug. Treating a variety of cancers is the main clinic effect of this drug, especially the effect in the treating of esophageal cancer and breast disease. Water extract and alcohol extract were its main effective parts and terpenoids were its main effective components. The data mentioned above will provide reliable literature information for the further studies of this plant.关键词：Hedyotis diffusa;chemical constituents;pharmacological actions;advance
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0