最新刊期
卷 20 ， 期 18 ， 2014
- 摘要：Summary of pharmaceutical research data of traditional Chinese medicine should present an overall profile of studies,analysis and self-evaluation,playing an important role for those applicants and reviewers who have a profound understanding of product and its manufacturing process.As booming acknowledge of new drug studies,based on these related guidelines and evaluation experience of authors,this paper gives suggestions contents and main consideration about summary of pharmaceutical research of new drug application for researcher and reviewers,including varieties profiles,prescriptions and accessories,production process,quality research and standards,inspection reports,stability test,instructions,packaging,labeling,analysis and evaluation,which will help to improve quality and efficiency of drug registration.关键词：new drug application of traditional Chinese medicine;summary of pharmaceutical research;writing proposals
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发布时间：2024-01-04 - 摘要：Objective: To investigate anti-tyrosinase and in vitro antioxidant activities of crude extracts from different parts of Blumea balsamifera,in order to screen active ingredients with whitening and antioxidant effects. Method: Functional leaves,tender leaves and immature stems of B. balsamifera were refluxed by aqueous,50% ethanol and 95% ethanol,respectively.Then crude extracts were measured tyrosinase inhibitory by enzymology method,and antioxidant activities were detected by DPPH,ABTS and FRAP methods. Result: DPPH free radical scavenging rate of 95% ethanol extract from functional leaves,tender leaves,immature stems of B. balsamifera was higher than other extracts,which was 95.38%,91.32% and 92.49%,respectively.FRAP,ABTS and anti-tyrosinase experiment's result showed that 50% ethanol and 95% ethanol extracts from functional leaves and immature stems possessed good activity.When concentration of each extracts were 1 500 mg·L-1,scavenging rates of aqueous,50% ethanol and 95% ethanol extracts of functional leaves for ABTS radical were 26.54%,82.48% and 67.46%,data of these three extracts of tender leaves were 34.15%,95.39% and 59.59%,while these data of immature stems were 52.05%,39.35% and 38.77%,respectively.Inhibition rates of aqueous,50% ethanol and 95% ethanol extracts of functional leaves on tyrosinase were 24.24%,32.79%,37.97%;these data of tender leaves were 27.12%,36.53% and 34.19%,while immature stems' were 31.36%,26.22% and 24.00%,respectively. Conclusion: Crude extracts from different parts of B. balsamifera have good antioxidant activities and inhibitory effect on tyrosinase,functional leaves and tender leaves are main active parts.关键词：Blumea balsamifera;antioxidant activities;DPPH radical;ABTS radical;iron reduction ability;inhibitory effect on tyrosinase
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发布时间：2024-01-04 - 摘要：Objective: To optimize rice wine steaming process of Schisandrae Sphenantherae Fructus. Method: According to contents of saponins,lignans,polysaccharides from Schisandrae Sphenantherae Fructus in composite score for index,Box-Behnken experimental design was adopted to investigate effects of wine content,soaking time and steaming time on processing technology,experimental data analysis was disposed by Design Expert 8.05 software,multivariate quadratic regression equation mathematical model between composite score and independent variables was established,then determined technical parameters by response surface methodology.UV was employed to determine contents of index components with detection wavelength of 540,560,484 nm,respectively. Result: Optimal processing parameters were as follows:added 11 g of wine for per 100 g of herbs,soaking time of 1 h,steaming time of 6.5 h;average contents of saponins,lignans and polysaccharides were 6.386%,12.297% and 4.659% with RSD of 2.31%,3.22% and 1.46%,respectively;composite score was 95.58 with deviation of 0.72% by comparing with the theoretical prediction value of 96.28. Conclusion: This optimized process can scientifically and reasonably control quality of Schisandrae Sphenantherae Fructus pieces.关键词：Schisandrae Sphenantherae Fructus;rice wine steaming process;Box-Behnken response surface analysis;Ginsenoside Re;schisantherin A;D-anhydrous glucose
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total polysaccharides from fruiting body of Phellinus igniarius and preliminary analyze its monosaccharide composition. Method: The content of total polysaccharides was determined by phenol-sulfuric acid method.With yield of total polysaccharides as index,single factor tests and orthogonal design were adopted to optimize extraction process by taking liquid-solid ratio,extracting time and times as factors,monosaccharide composition of total polysaccharides from different origin and species of P. igniarius was determined by TLC. Result: Optimum extraction conditions were as follows:extracted 5 times with 50 times the amount of water for 15 minutes each time.Yields of total polysaccharides in P. igniarius from Dongbei mulberry,Dongbei birch,Gansu mulberry and Hunan mulberry were 5.37%,2.21%,3.04% and 3.35%,they all contained glucose and galactose;yield of total polysaccharides in P. igniarius from Shandong mulberry was 2.77%,which contained xylose,galactose, glucose and lactose;yield of total polysaccharides in P. igniarius from un-know place was 3.58%,which contained glucose. Conclusion: Microwave extraction method is stable with high extraction rate.The content of total polysaccharides in P. igniarius from Dongbei mulberry is highest by comparing with other origin and species,TLC can preliminary determine total polysaccharides from fruiting body of P. igniarius all contain glucose.关键词：total polysaccharides from Phellinus igniarius;TLC;monosaccharide composition;phenol-sulfuric acid method
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发布时间：2024-01-04 - 摘要：Objective: To optimize supercritical CO2 fluid extraction process for reducing the content of estrogen in Ranae Oviductus. Method: The content of estrogen(estradiol and estriol) was determined by radioimmunoassay,taking average removal rate of estrogen in Ranae Oviductus as index,orthogonal design was adopted to optimize supercritical CO2 fluid extraction process with entrainer dosage,extraction temperature,time and pressure as factors. Result: Optimum process conditions were as follows:extracted 4 h at temperature of 50℃ and pressure of 30 MPa,with 35 mL of ethanol as entrainer;average removal rate of estrogen was 32.12%,surplus ratio of 1-methyl hydantoin was 88.7%. Conclusion: This optimized supercritical CO2 extraction process is stable and feasible,which has a good effect on reducing the content of estrogen in Ranae Oviductus and basically retains active ingredients for moistening lung to arrest cough.关键词：Ranae Oviductus;estrogen;supercritical CO2 extraction process;radioimmunoassay;estradiol;estriol
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发布时间：2024-01-04 - 摘要：Objective: To optimize alcohol precipitation process of extra-cellular polysaccharides in fermentative fluid of Cordyceps. Method: Phenol-sulfuric acid was adopted to determine the content of extra-cellular polysaccharides with detection wavelength at 489.4 nm.Taking the content of extra-cellular polysaccharides as index,orthogonal test was adopted to optimize alcohol precipitation process with ethanol amount,alcohol precipitation temperature and time as factors. Result: Optimum alcohol precipitation process parameters were as follows:alcohol precipitation for 7.5 h with 4 times the amount of 95% ethanol at 20℃;the content of extra-cellular polysaccharides was 0.671 g with purity of 69.32%. Conclusion: The content of extra-cellular polysaccharides in fermentative fluid of Cordyceps is high and can be utilized sufficiently.This optimized alcohol precipitation is reasonable and practical for industrial production of Jinshuibao capsules.关键词：fermentative fluid of Cordyceps;extra-cellular polysaccharides;phenol-sulfuric acid method;Jinshuibao preparations
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发布时间：2024-01-04 - 摘要：Objective: To investigate stability of Ganoderma spores oil experienced by six months accelerated test. Method: Taking acid value,peroxide value,contents of total triterpenoids and glycerol trioleate as indexes,quality changes of spores oil after 0,1,2,3,6 months accelerated test were investigated.The content of glycerol trioleate was determined by HPLC,which was performed on a Kromasil C18analytical column with mobile phase of acetonitrile-isopropanol (53:47) at flow rate of 0.5 mL·min-1,column temperature was 30℃,detector was evaporative light-scattering. Result: Compared with zero month,after 6 months accelerated test of Ganoderma spores oil,acid value showed a rising trend with rising 32.11%-39.27%;peroxide value showed a decreasing trend with decreasing 41.17%-46.67%;the content of total triterpenoids showed a decreasing trend with the range of 6.72%-8.13%;the content of glycerol trioleate showed a decreasing trend with dropping by 3.78%-4.54%;acid and peroxide values were accorded with gienic standard for edible vegetable oil during 6 months accelerated test. Conclusion: Quality of Ganoderma spores oil remains stable after 6 months accelerated test.关键词：Ganoderma spores oil;stability;accelerated test;glycerol trioleate;total triterpenoids
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发布时间：2024-01-04 - 摘要：Objective: To optimize alcohol extraction process of Shenqi Yiqi capsules. Method: With dry extract yield,contents of total ginsenosides and loganin as indexes,orthogonal design was carried out for optimizing extraction process by taking the concentration and amount of ethanol,extracting time and times as factors,optimum process was determined by multi-index test breakdown formula evaluation.Contents of total ginsenosides and loganin were determined by HPLC,mobile phase were consisted of acetonitrile(A)-water(B) in gradient elution(0-30 min,18.5%A;30-47 min,29%A;47-57 min,29%-40%A;57-65 min,40%-18.5%A) and acetonitrile-water (8:92),detection wavelength were set at 203,240 nm,respectively. Result: Optimal alcohol extraction technology was as following:extracted twice with 6 times the amount of 80% ethanol for 2.0 h per time;extracting amounts of total ginsenosides and loganin were 167.28 mg and 319.51 mg,dry extract yield was 22.54%. Conclusion: This optimized extraction process is reasonable and scientific,which can provide a reference for improving clinical efficacy of Shenqi Yiqi capsules.关键词：multi-index test breakdown formula evaluation;Shenqi Yiqi capsules;loganin;total ginsenosides;dry extract yield
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total phenolic acids in Capparis spinosa fruits. Method: Independent variables were ethanol concentration,solvent amounts and extracting time,dependent variable was yield of total phenolic acids,levels of independent variables were regressed multiple linearity and fitted by binomial,response surface methodology was used to optimize extraction process,then compared both of observed and predicted values. Result: Optimum extraction progress was as follows:extracted 2 twice with 15 times the amount of 70% ethanol for 1 h per time;yield of total phenolic acids was 7.29 mg·g-1,whose deviation was 0.68% by comparing with the predicated value(7.34 mg·g-1). Conclusion: This optimized process was simple with high precision and good predictability.关键词：Capparis spinosa fruits;central composite design-response surface methodology;total phenolic acids;protocatechuic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize microwave-assisted extraction process conditions of total alkaloids from Lycopodii Herba. Method: Acid dye colorimetry was employed to determine the content of total alkaloids with lycodoline as index component.Based on single factor tests,effects of liquid-solid ratio,extraction temperature and time on yield of total alkaloids were investigated with a three-factor and three-level Box-Behnken central composite design,microwave-assisted extraction conditions of total alkaloids from Lycopodii Herba was optimized by response surface methodology and compared with traditional extraction technique. Result: Optimum conditions were as follows:extracted 8 min with 17 times the amount of methanol-water-hydrochloric acid (85:15:1) at 72℃;yield of total alkaloids was 4.092 mg·g-1 under these conditions,which was consistent with the theoretic value of 4.070 mg·g-1. Conclusion: Microwave-assisted extraction process has many advantages over conventional methods,such as time saving,high extraction ratio and low solvent consumption.关键词：Lycopodii Herba;microwave-assisted extraction;total alkaloids;response surface methodology;lycodoline
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发布时间：2024-01-04 - 摘要：Objective: To optimize extracting technology of Rehmanniae Radix in Shiduqing capsules and compared its pharmacodynamic action before and after being processed. Method: HPLC was employed to determine the content of catalpol with mobile phase of acetonitrile-0.1% phosphoric acid (1:99) and detection wavelength at 210 nm.With extracting amount of catalpol as index,orthogonal test was adopted to optimize extraction technology by taking liquid-solid ratio,grinding degree and extracting time as factors.With physiological saline as blank control and dexamethasone as a positive control,pharmacodynamic action of Rehmanniae Radix before and after being processed was compared by anti-inflammatory and analgesic test. Result: Optimum extracting technology was as following:shattered into 5 mm small pieces and extracted twice with 20 times the amount of water for 1.5 hours per time.Before and after being cut,inhibition rates for xylene-induced auricle swelling in mice of high-dose(16 g·kg-1) groups were 33.72% and 48.34%,while low-dose groups were 43.13% and 64.31%,respectively. Conclusion: This optimized extracting technology is stable and feasible,which provides a reference for industrial production of Shiduqing capsules.关键词：Shiduqing capsules;Rehmanniae Radix;catalpol;anti-inflammatory and analgesic trial
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发布时间：2024-01-04 - 摘要：Objective: To optimize extracting process of total flavonoids from acid Litchi Semen. Method: Taking rutin as standard,the content of total flavonoids was determined by UV.With yield of total flavonoids as index,on the basis of single factor tests,Box-Behnken and Plackett-Burman tests were adopted to optimize extraction process by taking temperature,grinding degree,ethanol concentration,liquid-solid ratio,extracting time and stirring speed as factors. Result: Grinding degree and ethanol concentration were significant factors,optimum extraction technology was as following:grinding degree of 120 mesh,extracted 2 h with 14 times the amount of 75% ethanol at 60℃,stirring speed of 90 r·min-1.Under these conditions,average extraction rate of total flavonoids was 17.49% with RSD of 0.63%. Conclusion: This optimized technology is stable and feasible,which is suitable for industrial production of anti-hepatitis B flavonoids from acid Litchi Semen.关键词：response surface methodology;acid Litchi Semen;anti-hepatitis B component;total flavonoids
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发布时间：2024-01-04 - 摘要：Objective: This study was aimed to estabilish a high-performance liquid chromatography (HPLC) method for HPLC characteristics of aqueous extract of Zhenwu decoction (ZWT) and simultaneous determination of makers-characteristic chromatography, benzoylmesaconine, benzoylaconine, atractylenolide Ⅲ, paeoniflorin and 6-gingerol. Method: The separation was carried out on a Phenomenex Luna C18(4.6 mm×250 mm, 5μm) column eluted with mobile phases of acetonitrile(A) and water containing 0.2% phosphoric acid(B) in gradient mode at the flow rate of 1.0 mL·min-1.The column temperature was maintained at 30℃ and the detection wavelength was set at 230 nm. Result: The obtained HPLC characteristics containing 36 common peaks, which were in well shape and high resolution. Five constituents of ZWT were analyzed and quantified paeoniflorin(1.410±0.024) g·L-1, benzoylmesaconine(0.066±0.001) g·L-1, benzoylaconine(0.015±0.001) g·L-1, atractylenolide Ⅲ(0.028±0.003) g·L-1, 6-gingerol(0.069±0.001) g·L-1, with the linear range as follows Y=25.907X+9.741 (r=0.999 7), Y=15.265X-3.202 (r=0.999 5), Y=15.660X-4.699 (r=0.999 1), Y=63.73X-10.740 (r=0.999 6), Y=21.645X-4.074 (r=0.999 5), respectively. The precision(1.72%, 1.76%, 2.78%, 1.20%, 1.05% for RSD of peak area, 0.81%, 0.47%, 0.43%, 0.07%, 0.05% for RSD of retention time), repeatability(1.95%, 2.56%, 2.93%, 2.65%, 3.01% for RSD of peak area;0.03%, 0.19%, 0.28%, 0.11%, 0.12% for RSD of retention time), stability (1.05%, 1.36%, 2.89%, 2.81%, 2.64% for RSD of peak area;0.15%, 0.12%, 0.39%, 0.04%, 0.08% for RSD of retention time), and average recovery (99.85%, 101.67%, 101.91%, 99.82%, 101.44%, respectively) of this method were all satisfied. Conclusion: The method established in this study could be used in simultaneous determination of the five makers of zhenwu decoction and could be used to control the quality of ZWT aqueous extract.关键词：Zhenwu Decoction;characteristic chromatography;benzoylmesaconine;benzoylaconine;Atractylenolide Ⅲ;paeoniflorin;6-gingerol
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发布时间：2024-01-04 - 摘要：Objective: This study was aimed to develop an UPLC method to determine the contents of chlorogenic acid, caffeic acid, baicalin, wogonoside, baicalein and wogonin in Yinhuang granule. Method: Six active components were separated on an ACQUITY UPLC BEH C18column(2.1 mm×50 mm,1.7 μm)eluted with acetonitrile and water containing 0.2% phosphoric acid as mobile phases in a linear gradient mode. The detection wavelength was set at 326 nm. Result: The linear ranges were 0.000 107-0.107 μg for chlorogenic acid, 0.000 244-0.244 μg for caffeic acid, 0.000 272-0.272 μg for baicalin, 0.000 059 0-0.059 0 μg for wogonoside, 0.000 053 0-0.053 μg for baicalein, 0.000 051 0-0.051 μg for wogonin,respectively. The average recoveries of the six components were 96.34%(RSD 0.90%),101.4%(RSD 1.53%), 95.64%(RSD 1.34%), 98.01%(RSD1.72%),98.61%(RSD 1.96%), 102.7%(RSD 1.42%), respectively. Conclusion: The method was proven to be simple, accurate, reliable and can be used for quality control of the six flavones inYinhuang granule.关键词：UPLC;Yinhuang granule;chlorogenic acid;caffeic acid;baicalin;wogonoside;baicalein;wogonin
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发布时间：2024-01-04 - 摘要：Objective: Analysis and comparison the paeonol and paeoniflorinon different part of one Feng-Cortex Moutan by HPLC. Method: Using the Waters VP - ODS - C18 chromatographic column (4.6 mm×150 mm,5 μm).Mobile phase for the paeonol: methanol-water (55∶ 45), detection wavelength 274 nm, column temperature 30℃, the flow rate of 1.0 mL·min-1. Mobile phase for thepaeoniflorin: acetonitrile - 0.1% H3PO4 (20:80),detection wavelength 230 nm, column temperature 30℃, the flow rate of 1.0 mL·min-1. Result: Paeonol and paeoniflorin had good linear relationship in 1.073-5.365 μg and 0.593-2.965 μg, the ‘r' was 0.999 9 and 0.999 1, the average recoveriey for the paeonol was 99.96% (RSD 0.72%), the average recovery for the paeoniflorin was 99.03% (RSD 0.49%). Conclusion: The experiments show that the content of the paeonol and paeoniflorin localed at the thinest part of the chosen Feng-Cortex Moutan is much more higher than the rest part.关键词：Feng-Cortex Moutan;paeonol;paeoniflorin;different parts
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发布时间：2024-01-04 - 摘要：Objective: This investigation aimed to study the chemical constituents of Bletillae Rhizoma. Method: Normal phase silica gel, MCI, and Sephadex LH-20 column chromatographies and semi-prep HPLC were used to isolate and purify the chemical constituents. Their structures were identified by spectroscopic methods, including 1H-NMR, 13C-NMR and ESI-MS. Result: Seven compounds were isolated and identified as α-isobutylmalic acid (1), 4,4'-dihydro xydiphenylmethane (2), spicatolignan B(3), gymnosides V (4), 4,4'-dihydroxybenzyl sulfide (5), gymnoside IX (6) and gymnoside X (7). Conclusion: All compounds were isolated from Bletilla for the first time.关键词：Bletillae Rhizoma;chemical constituents;isolation and purification;structura1 identification
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发布时间：2024-01-04 - 摘要：Objective: The study aimed to the best harvest time through determining the content of rosmarinic acid, total diterpenoids and total flavonoids in the second cutting Linearstripe Rabdosia Herb, as well as to provide basic researching data to formulate relevant standard operating procedure (SOP) according to good agricultural practice (GAP) of Chinese medicinal materials and put them into practice. Method: HPLC and ultraviolet spectrophotometry were used to determine the content of rosmarinic acid, total diterpenoids, and total flavonoids in the second cutting Linearstripe Rabdosia Herb at different harvest time from Linearstripe Rabdosia Herb plantation base. Result: The content of rosmarinic acid varied greatly, with the difference as much as up to 10 times, while the difference of content of total diterpenoids and total flavonoids were both relatively small in the second cutting Linearstripe Rabdosia Herb at different harvest time. The contents of three active compounds were positively correlated with harvest time in Rabdosia serra, however the contents of three active compounds were fluctuated during investigation period in R. lophanthoides and R. lophanthoides (Buch.-Ham.ex D.Don) H.Hara var.graciliflora (Benth.) H.Hara. The author suggested that the best harvest time for the second cutting R. serra, R. lophanthoides, R. lophanthoides (Buch.-Ham.ex D.Don) H.Hara var. graciliflora (Benth.) H.Hara are at the 120-140th day and at the 60th day after first cutting, respectively. Conclusion: The two determination methods are simple, rapid, accurate and repeatable. All the samples used in this study were collected from Linearstripe Rabdosia Herb planting base, which are representative to evaluate the resource utilization value of the second cutting Linearstripe Rabdosia Herb. This study is of some significance to guiding the harvest of the second cutting Linearstripe Rabdosia Herb and building the Linearstripe Rabdosia Herb GAP planting base.关键词：Linearstripe Rabdosia Herb;the second cutting;rosmarinic acid;total diterpenoids;total flavonoids
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发布时间：2024-01-04 - 摘要：Objective: This study was to provide the experimental foundation for reasonable utilization of Poriae Cutis by analyzing the volatile constituents from it. Method: The volatile oil was extracted from the rind of Poriae Cutis by steam distillation. The chemical constituents were analyzed by GC-MS. Result: A total of 104 compounds were separated and 67 of them were identified, which accounted for 79.69% of all the volatile oil. The major components were d-cadinene(9.360%), α-muurolene(6.780%), α-amorphene(4.898%), 1-b-bisabolene(4.756%), nerolidol(3.479%), d-longifolene(3.243%) and α-calacorene(3.041%). Conclusion: This paper reported, for the first time, the composition of volatile oils of Poriae Cutis by GC-MS.关键词：Poriae Cutis;Steam Distillation;GC-MS;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: To study anti-tyrosinase and antioxidation activities of extract from Oroxylum indicum. Method: This paper took different polar fractions of extract from the O. indicum ethanolic extract as research objects, measured its inhibition of tyrosinase and 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·) and the hydroxyl free radical (·OH) scavenging ability. Result: Its alcohol extract of ethyl acetate extract (EAO), n-butanol extract (n-BO) and water extract (WO) had inhibition to tyrosinaes, the values of IC50 reached 3.081 4,8.525 3, 22.750 8 g·L-1 respectively;EAO, n-BO,WO had stonger scavenging effect of DPPH·and·OH, the order of scavenging ability was EAO>n-BO>WO. Conclusion: O. indicum. had better tyrosinase inhibitory and antioxidation, its antioxidation ability had positive correlation with its anti-tyrosinase. Its functional components were mainly distributed in the ethyl acetate phase.关键词：Oroxylum indicum;tyrosinase;inhibition;free radical;scavenging;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to analyze the volatile constituents from the aerial part and the underground part of Piper sarmentosum, toprovide a basis for its development and clinical application. Method: The refluxing method was used to extract the chemical constituents from the root and the aerial part of P.sarmentosum with petroleum ether and dichloromethane. The components were separated and identified by GC-MS. Result: Thirty-six components in aerial part and 32 components in underground part were identified. Both the underground part and aerial part contained 20 common constituents, such as hydrocinnamic acid, α-cubebene, humulene,β-caryophyllene, β-asarone, α-asarone, isoelemicin and so on, which accounted for more than 85% and 55% of the total content of the aerial part and underground part, respectively. And α-asarpneshowed the highest content in each part, which is more than 30%. The aerial part contained 16 specific componentssuch ascalarene,α-selinene,d-cadinene,β-eudesmol,cis-nerolidol,5-octadecene,squalene and so on; whilethe underground part contained 12 specific components such as (1R)-(+)-α-pinene, β-pinene,3-phenyl-propanamide and so on. Conclusion: Most of the volatile constituents from the aerial part and underground part of P.sarmentosumare identical,but their contents are obviously different.Andthere are specific constituents in both two parts, which might relate to theirdifferent medical usage.关键词：Piper sarmentosum;volatility constituents;GC-MS;above the ground part;under ground part
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发布时间：2024-01-04 - 摘要：Objective: The study was conducted to establish a HPLC method for simultaneously determination of rutin, naringin, hesperidin, neohesperidin and quercetin in Jinggu tablet. Method: The separation of compounds was performed on Elite Kromasil-C18 column (4.6 mm×250 mm, 5 μm), with the mobile phase consisted of acetonitrile-0.2% acetic acid. The flow rate was set at 1.0 mL·min-1and detection wavelengths were set at 254 nm for rutin and quercetin, 283 nm for naringin, hesperidin and neohesperidin. Result: The samples showed a good relationship in the ranges of 0.012 5 -0.500 4 g·L-1 for rutin, 0.010 0-0.400 8 g·L-1 for naringin, 0.003 3-0.032 7 g·L-1 for hesperidin, 0.011 3-0.225 6 g·L-1 for neohesperidin and 0.004 1-0.081 3 g·L-1 for quercetin. All of the average recoveries were between 98% and 102%. Conclusion: The method is accurate, reliable and reproducible and can be used for the quality control of Jinggu tablet.关键词：HPLC;Jinggu tablets;rutin;naringin;hesperidin;neohesperidin;quercetin
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study wasto compare the chemical constituents of Radix Podophylli and Fructus Podophylli, and analyze the relationship between chemical constituents and the toxicity, as well as to provide the basis for establishment of the fingerprint. Method: HPLC fingerprint was used to study the chemical composition of Radix Podophylli and Fructus Podophylli. The relationship between chemical composition and toxicity was analyzed through acute toxicity test. Result: There were 14 common chromatographic peaks for Radix Podophylli and Fructus Podophylli, the contents of Podophyllotoxin and 4'-demethylpodophyllotoxin in Radix Podophylli were higher thanin Fructus Podophylli. Median lethal dose for Radix Podophylli and Fructus Podophylli was 0.088 7,0.909 8 g·mL-1, repectively. Conclusion: Radix Podophylli and Fructus Podophyllihave the same chemical composition. But there is a great difference in content. Radix Podophylli hasstronger toxicity than Fructus Podophylli. The content of toxic components Podophyllumshould be strictly controlled in the formulation of medicine quality standard.关键词：Radix podophylli;Ructus podophylli;HPLC fingerprint;Acute toxicity test
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish a rapid method to analyze the volatile components of Artemisiae Argyi Folium and to study the content and distribution characteristics of Artemisiae Argyi Folium from different habitats. Method: The volatile components were investigated by HS-SPME-GC-MS based on the agilent productivity Chem Station and were quantified roughly by peak area normalization method.The data were analyzed by principal component analysis(PCA) and cluster analysis(CA). Result: Eighty-four components were identified preliminarily,which were mainlyketones, alkenes, aldehydes and alcohols. There were significant differences in essential components of Artemisiae Argyi Folium from different habitats. It can be distinguished effectively by principal component analysis and cluster analysis.The quality of Artemisiae Argyi Folium from Qichun of Hubei province was the best,followed by from Guangbu of Hunan province and Tangying of Henan province.The results of the cluster analysis revealed five main clusters. Conclusion: The method is reliable and stable. This method can be applied to analyze the volatile components of Artemisiae Argyi Folium. It will provide the scientific basis for quality assessment of Artemisiae Argyi Folium.关键词：Artemisiae Argyi Folium;volatile components;HS-SPME-GC-MS;principal component analysis;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: The pupose of this study was to establish a rapid detection method for six chemical constitutes illegally added into health foods for diet. Method: This method used on the ultra-performance liquid chromatography-tandem mass spectrum (UPLC-MS-MS) technology,in which an Acquity BEH-C18 column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase of 0.1% formic acid and acetonitrile by gradient elution.The target compounds were analyzed through electrospray ionization, selective ion scan(SIR) and multiple-reaction monitoring(MRM) modes. The compounds illegally added into slimming health foods were qualitatively identified and quantitatively determined according to the quasi-molecular ion peaks and the MRM chromatography peaks by comparison with those of reference substances.This method was used for analysis of 25 batches of health foods for diet. Result: Sibutramine hydrochloride and phenolphthalein were found in 6 batches of slimming health foods. Conclusion: The method is of great specificity, and sensitivity and can be used to detect the chemical compositions into the slimming health foods.关键词：UPLC-MS-MS;selective ion scan;multiple-reaction monitoring;health foods for diet;illegally addition
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发布时间：2024-01-04 - 摘要：Objective: This article was aimed to establish a rapid identification method for the detection of illegally added pigments in Carthami Flos besides golden orange pigment Ⅱin the case of that only golden orange pigment Ⅱcan be detected according to the current national inspection approval of to supplement the inspection of illegally added pigment in Carthami Flos besides golden orange pigment Ⅱ. Method: In TLC method, silicagelG was used as stationary phase, 70% ethanol was used as solvent, and ethylacetate-butanol-ethanol-ammonia-water (1:3:3:1:1) was used as developing solvent, and the result was detected under visible light. The positive samples were further proved by HPLC method. In HPLC method,octadecylsilane bonded silica was used as a filler, methanol-0.05 mol·L-1 ammonium acetate(52∶ 48) was used as mobile phase. The column temperature was 35℃,the detection wavelength was 508 nm, by tested twice with ultraviolet detector and photodiode array detector.Then the result was compared with the peak of the control reagents for further confirmation. Result: The results of two positive samples by TLC were consistent with those by HPLC. Two out of nine groups of Carthami Flos samples were proven to contain the forbidden food pigment acid red 73. Conclusion: The method is suitable for rapid screening of illegally added pigment in Carthami Flos. The established TLC method is simple, rapid, reproducible, cost-effective, to supplement the relevant provisions of national standards, and to play a significant role in the management of medicine timber and Chinese Herbal Medicine.关键词：Carthami Flos;illegal add pigment;TLC;acid red 73;drug testing vehicles
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发布时间：2024-01-04 - 摘要：Objective: To compare the content of four compounds in the flower buds and leave of Daphne genkwa. Method: Apigenin, luteolin, hydroxyl genkwanin, genkwanin were used as reference. The contents of the four compounds in the flower buds and leaves were detected by RP-HPLC method. The difference between the two parts were analyzed according to the data. Kromasil C18 column (4.6 mm × 250 mm, 5μm); mobile phase of methanol - water (58:42:0.5), flow rate 0.8 mL·min-1, the detection wavelength of 350 nm (luteolin, hydroxyl genkwanin), 338 nm (apigenin, genkwanin), the column temperature was set at 35℃. Result: The levels of these four components yuanhuacine were: 0.017%, 0.088 4%, 0.069 9%, 0.059 3%. Daphne leaves the content of these four components as follows: 0.011 8%, not detected, 0.012 3%, not detected. Conclusion: Studies showed that there were different compounds in the flower buds and leaves of the drug, so they should be used in clinic separately according to different clinical purpose.关键词：flower buds of Daphne genkwa;leave of D. genkwa;apigenin;luteolin;hydroxyl genkwanin;genkwanin
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发布时间：2024-01-04 - 摘要：Objective: To establish the method of HPLC about the content of vitamin E in dexamethasone acetate cream and to determine the content of vitamin E in dexamethasone acetate cream. Method: The qualitative analysis was performed on Inertsil ODS-SP(4.6 mm×150 mm,5 μm).The mobile phase was methanol-water(98:2) at the flow rate of 1.0 mL·min-1,the detection wavelength was 284 nm and the column temperature was 40℃. Result: The concentration of vitamin E showed a good liner relationship in the range of 0.029 6-0.296 mg·L-1(r=0.999 9),and the average recovery was 99.70%-101.80%,RSD 0.9%(n=6). Conclusion : After verifying the method, the method is accurate with good reproducibility and can be used for the qualitative analysis of vitamin E in dexamethasone acetate cream.关键词：vitamin E;HPLC;qualitative analysis;dexamethasone acetate cream
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determination of gastrodigenin content in brain tissue of SHR rats and investigate its tissue distribution before and after compatibility of Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis. Method: HPLC was employed to determine the content of gastrodigenin in brain tissue with mobile phase of acetonitrile-0.03%H3PO4 (7:93) and detection wavelength at 221 nm.Distribution of gastrodigenin in brain tissue before and after compatibility of Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis was detected. Result: The linear range of calibration curve was within drug concentrations of 0.5-100 mg·L-1(R2=0.999 9),recoveries of three levels were more than 74.89% and accuracies were more than 98%.Pharmacokinetic parameters of gastrodigenin in brain tissue before and after compatibility were received,such as Cmax were 0.485,0.373μg·g-1,AUC were 111.366,213.585 μg·g-1·min,respectively. Conclusion: This study establish a sensitive and accurate method for determination of gastrodigenin in brain tissue of SHR rats.Compatibility of Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis show a significant effect that first inhibiting and then promoting on distribution of gastrodigenin in brain tissue.关键词：Gastrodiae Rhizoma;gastrodigenin;brain tissue distribution;Uncariae Ramulus Cum Uncis
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发布时间：2024-01-04 - 摘要：Objective: To study the absorption of isochlorogenic acid A in rat intestine. Method: The absorptions of three concentrations (18, 58, 100 mg·L-1) of isochlorogenic acid A in different intestinal segments were studied with phenol red as the marker by in situ rats single pass perfusion model. Result: Effective permeability(Peff) of duodenum, jejunum, ileum and colon in different concentration of isochlorogenic acid A was in (0.69-6.64)×10-5cm·s-1, and there was upgrade tendency between the Peff of duodenum, jejunum, ileum and colon in different concentration of isochlorogenic acid A, and it has obvious difference among each concentration. The Peff in the ileum obviously higher than that in the duodenum, jejunum and colon, namely the ileum was the best absorption section. Conclusion: The absorption of isochlorogenic acid A was not saturated in high concentration, indicating that the transport mechanisms of isochlorogenic acid A was passive diffusion, and had a special absorption window.关键词：isochlorogenic acid A;single-pass intestinal perfusion model;absorption
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of beta-asarone on the behaviors and the expressions of mitogen extracellular kinaes(MEK)and extra cellular signal-regulated protein kinase (ERK) in the hippocampus of depression model rats. Method: Eighty adult Sprague-Dawley rates were divided into 4 groups randomly:the normal control group;the model group;the fluoxetine control group;the β-asarone group,20 in each one. Solitary and chronic mild unpredictability were used to stimulation on each one except the normal group.On the second day of a successful model,the fluoxetine and the β-asarone group were given corresponding drugs once a day by gastrogavage for 21 days(1.2 mg·kg-1·d-1 to the fluoxetine control group,25 mg·kg-1·d-1 to the β-asarone group).At 1,7,14,21 days,weight was measured,Open-field-test and sucrose consumption volume were detected,Real-time PCR was used for quantitative analysis of MEK and ERK mRNA. The protein expression of MEK and p-ERK in the hippocampus were tested by immunohistochemical staining method. Result: Compared with the normal group,the weight of the rats in the model group was decreased,on the 14th day,the rats lose weight obviously(P<0.05).The scores of Open-field-test and sugar water preference were reduced significantly since the 7th day of the experiment(P<0.01).The level of MEK,ERK mRNA were decreased(P<0.01),and the protein expressions of MEK,p-ERK were declined(P<0.05).Compared with the model group,the scores of β-asarone group in the Open-field-test and sucrose consumption volume were improved.The changes of ratsweight was found on the 14thday and 21th(P<0.05).The scores of Open-field-test increased until 14th day(P<0.01).The levels of MEK and ERK mRNA were Up-regulated(P<0.01),the protein expressions of MEK and p-ERK were increased (P<0.05)in the hippocampus.Similar effects were observed in fluoxetin group. Conclusion: Beta-asarone could alleviate the symptoms of depression in rats effectively,its mechanism might be related to increasing the protein expression of MEK and p-ERK in the rat hippocampus.关键词：beta-asarone;hippocampus;depression;mitogen extracellular kinaes;extra cellular signal-regulated protein kinase
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发布时间：2024-01-04 - 摘要：Objective: To explore the expression of Janus kinase 2(JAK2) and signal transducer and activator of transcription 3(STAT3) in hepatic fibrosis rat model impacted by Xiaochaihu Tang. Method: Hepatic fibrosis model was induced by 10% CCl4 for 9 weeks in rat. The rats were treated by Xiao Chaihu Tang for 6 weeks at the beginning of the 9th week. Alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were measured to reflect liver function. The pathological characteristics of hepatic tissues were detected by HE staining. Tissue inhibitor of metalloproteinase-1 was observed by immunohistochemistry. The expression of JAK2 and STAT3 mRNA was detected by real-time RT-PCR. Result: Compared with the model group, serology and histology showed significant improvement. TIMP-1 was reduced in the high-dose group of Xiaochaihu Tang compared with the model group and the expression of JAK2 and STAT3 was downregulated obviously in the middle-dose group (P<0.05) and the high-dose group (P<0.01). Conclusion: Xiaochaihu Tang can improve the liver fibrosis induced by CCl4 and depress the expression of JAK2 and STAT3 in rat.关键词：hepatic fibrosis;Xiaochaihu Tang;TIMP-1;Janus kinase 2/ signal transducer and activator of transcription;signal pathway
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Yiye Anti-influenza capsule (YYAFC) on the resistance to influenza A/H1N1 virus. Method: ICR mice were randomly divided into control group (0.5%CMC, 20 mL·kg-1), ribavirin(0.07 g·kg-1), influenza virus particles group (4.24 g·kg-1) and YYAFC group(1.5, 0.75, 0.38 g·kg-1). The pneumonic model of mice were infected by influenza A(H1N1) with nasal drops, mice were administrated after infection virus in treatment andprecautiongroups. The lung index, death rate, death protection rate, the average survival period and life prolongation were calculated;cytopathic effect(CPE) was used to evaluate the anti-influenza virus(H1N1) effect of YYAFC in vitro. Result: Compared to normal group, mice were infected with the A/H1N1 influenza virus (FM1 and PR8), the lung indexin treatment and precaution groupswassignificantly increased (P<0.01), death rate was 90% and 90% in treatment group and 85%and 55% in precautiongroup, respectively,the average survival period was 9.20 days and 9.25 days in treatment griups and 9.35 days and 11.60 days in precaution group. In the treatment group of high and medium dosage YYAFC, the lung index decreased significantly, while the life span of mice was prolonged.In the precaution group of high and medium dosage YYAFC, the life span of mice was prolongedsignificantly. The IC50 of the sample inhibiting CPE caused by FM1 in vitro with doses of 0.89 g·L-1 and the TI was 6.75. Conclusion: YYAFC showed inhibitory effect on influenza A virus FM1 stain in vivo and in vitro.关键词：Yiye Anti-influenza capsule;influenza virus;viral pneumonia;Madin-Darby canine kidney cell
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of rhynchophylline(Rhy) on glutamate receptor 1 (GluR1) subunit in hippocampus of methamphetamine-induced conditioned place preference (CPP)mice. Method: Mice were randomly divided into five groups randomly with 10 mice in each group:normal control group, methamphetamine model group, methamphetamine with Rhy (40 mg·kg-1), methamphetamine with Rhy (80 mg·kg-1), methamphetamine with ketamine (15 mg·kg-1). Each respective drug was administered daily for 4 days. Established place preference mice models were induced by methamphetamine,to observe the expression of GluR1 was observed by immunohistochemistry technique and Western blot. Result: Compared with normal control group, the time of mice with methamphetamine model group in non-preferred compartment increased significantly (P<0.01), indicating that methamphetamine place preference model was successfully established. Compared with methamphetamine model group, ketamine (15 mg·kg-1),Rhy (40 mg·kg-1)and Rhy (80 mg·kg-1) reduced the time spent on white compartment of CPP mice significantly(P<0.01).Immunohistochemistry showed that the expression of GluR1 in hippocampus was increaseding in the methamphetamine model group(P<0.01), while in the ketamine group, low and high doseage Rhy were decreasing(P<0.01);Western blot showed that the express of GluR1 protein was significantly increased in the model group(P<0.01), while in the ketamine group, low and high doseage Rhy group were significantly decreasing(P<0.01). Conclusion: GluR1 protein plays an important role in the formation of methamphetamine-induced place preference in mice.关键词：rhynchophylline;methamphetamine;conditioned place preference;glutamate receptor 1;hippocampal
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发布时间：2024-01-04 - 摘要：Objective: The purpose of the research is to observe inhibition of Tonglian decoction (TD) on nude mice modeled with gastric cancer cells MGC803 and on proliferating cell nuclear antigen (PCNA) expression in tumor tissue, to provide experimental basis for clinical application of TD. Method: MGC803 cells were inoculated subcutaneously in right forelimbs of all mice. Mice in model group were treated with saline solution 10 mL·kg-1 orally. Mice in positive control group were treated with xiaoaiping tablet at dosage of 10 mL·kg-1, 1.80 g·kg-1 (crude drug) of liquid. Mice in TD groups were subdivided as high (1.50 mg·kg-1), medium (0.75 g·kg-1) and low (0.37 g·kg-1) dose group of drug liquid, with dosage of 10 mL·kg-1. All mice were administrated for 3 weeks and were observed for another 3 weeks continuously. Tumor volumes were measured for 6 weeks every day from the beginning of MGC803 cells transplanted. All mice were killed for isolation of tumor tissue, inhibition ratio of tumor-weight measured. Finally, PCNA expression was examined with immunohistochemistry. Result: TD inhibits tumor growth with positive correlation with time, the inhibitory peak appeared at 4 weeks after medication;tumor weight in TD high-dose group was (1.55±0.68) g, and the inhibition ratio was (58.40±1.56)%, which was significantly different from that in the model group (P<0.05). Positive rate of PCNA expression aslo decreased significantly, showing statistical difference from the model group (P<0.05). Conclusion: TD inhibits tumor growth on nude mice modeled with MGC803, through down regulation of PCNA protein expression.关键词：Tonglian decoction;gastric cancer;MGC803;nude mice transplanted with tumor;proliferating cell nuclear antigen
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发布时间：2024-01-04 - 摘要：Objective: To study the spectrum-effect relationship on anti-inflammatory effective fraction of Echinopsis Radix and the basis for its anti-inflammatory effect of substance. Method: Mice ear swelling experiment was used to observe its anti-inflammatory effect of different polar parts and to screen efficacy site preliminarily. The HPLC fingerprints of its anti-inflammatory effective fraction were established. Each component peak areas of HPLC fingerprints and anti-inflammatory efficacy were associated by the grey correlation analysis. A multiple linear regression analysis was conducted by the backward method to establish a fit regression equation. Result: The chloroform part of Echinopsis Radix had the most significant anti-inflammatory effects. There were significant differences compared with the model group (P<0.01), and there were not significant differences compared with the positive control group. In the nine peaks of the chloroform part fingerprint of Echinopsis Radix, there were seven peaks which had a significant contribution to the anti-inflammatory effect. The compounds represented by the 2nd, the 3rd, the 5th and the 7th peaks(the 7th peak represent α-triple thiophene)had anti-inflammatory effect by a positive correlation, but the compounds represented by the 4th, the 8th and the 9th peaks had anti-inflammatory effect by a negative correlation. Conclusion: The chloroform part of Echinopsis Radix has a good anti-inflammatory effect. One of the main active ingredients of anti-inflammatory effect of Echinopsis Radix is α-triple thiophene. In addition, there are six major ingredients involved in this pharmacological process.关键词：Echinopsis Radix;anti-inflammatory effective parts;fingerprints;spectrum-effect relationship
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发布时间：2024-01-04 - 摘要：Objective: To study blood sugar and glycosylated hemoglobin of different doses and different compatibility of effective parts from Jingluo Shuning capsule. Method: A single intraperitoneal injection of streptozotocin (48 mg·kg-1) was used to induce diabetic rats. The modling rats were randomly divided into control group, modelgroup,Jingluo Shuning groups(34.4,68.7,137.4 mg·kg-1)and the 9 compatility groups according to the combination of three factors and three levels orthogonal design ratio. The oral administration lasted for 28 days. The fasting blood glucose levels and glycosylated hemoglobin(GHb) were measured. Result: The middle dose group had significant effect.On this basis, the different dosage group and two two compatibility were compared, the results showed the optimal dose. Finally,in the formula of the three effective parts the combination 5,indicated the best effect. Conclusion: In Jingluo Shuning effective parts combination 5 has the most significant effect on the blood sugar and GHb.关键词：Jingluo Shuning capsule;effective parts;blood glucose;glycated hemoglobin
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发布时间：2024-01-04 - 摘要：Objective: To study the antibiosis and antiphlogosiseffects of Qianlie Miniao Shuan on acute prostatitis. Method: Sixty mice were randomly divided into six groups:blank group,model group,positive group and high, middle,low dosage groups of Qianlie Miniao Shuan (0.25,0.19,0.13 g·kg-1). The model was induced by bacterial infection of Proteus(clinical isolates) or Escherichia coli (clinical isolates) in vivo by intraperitoneal injection after three days. The drugs were given for 5 days after mice infected 24 hours. The protective effect of mouse death was observed by the mouse model infected by E. coli or Bacillus proteus in vivo Qianlie Miniao Shuan dosing. The Petri dishes mixed qianlie miniao shuan solution and serum broth were made of different drug concentration(250.8,188.1,125.4,62.7,31.4 g·L-1),each concentration was 4 Petri dishes.After added broth dilution, the Petri dishes were cultured at 37℃ for 48 h,and then we observed minimal bactericidal concentration(MBC) and minimal inhibitory concentration(MIC).The antibacterial and bactericidal effect of Qianlie Miniao Shuan on the main pathogens of acute prostatitis was observed by the antibacterial test in vitro. The anti-inflammatory effect of Qianlie Miniao Shuan was observed by the acute prostatitis model in rats infected by E. coli or B. proteus. Result: The mortality rates of the mice infected Proteus infection or E. coli given Qianlie Miniao Shuan in the three dosages groups were lower than the model group(P<0.05).The MBC of Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, E. coli, Proteus (clinical isolates)and E. coli (clinical isolates) were 62.7, 125.4, 250.8, 188.1, 250.8, 188.1 g·L-1, The MIC of S. aureus, Salmonella, P. aeruginosa, E. coli, Proteus (clinical isolates)and E. coli (clinical isolates) were 31.4, 62.7, 188.1, 125.4, 188.1, 125.4 g·L-1.The prostate organ coefficient of acute prostatitis model caused by Proteus or E. coli gived qianlie miniao shuan were less than the model group,the difference had statistical significance(P<0.05).The pathological results showed the tissue renewing in the three dose groups was better than that in the model group.The Qianlie Miniao Shuan could significantly reduce the inflammatory infiltration of prostate tissue in rats(P<0.05). Conclusion: Qianlie Miniao Shuan for acute prostatitis has good antibacterial and anti-inflammatory effects.关键词：Qianlie Miniao Shuan;acute prostatitis;in vivo antibacterial;in vitro antibacterial;anti-inflammatory effect
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Maca polysaccharide on the life span and antioxidative ability of Drosophila melanogaster. Method: Eight hour eclosion unmated D.melanegaster were collected.Male and female were separated,D.melanogaster were randomly divided into 4 groups:control group,Maca polysaccharide group (0.03, 0.08, 0.24 g·L-1). The life span,the survival time in paraquat and hydrogen peroxide treatment, the activities of superoxide dismutase (SOD), catalase(CAT)and the content of lipofuscin were determined. Result: Compared with the control group, the high dose group extends the maximum life span and mean life span of D. melanogaster(P<0.05 or P<0.01), exhibiting a dosage effect. The middle dose group and the high dose group can improve the survival time in paraquat and hydrogen peroxide treatment. The activities of SOD and CAT increase,meanwhile the content of lipofuscin decreases in the experiment. Conclusion: Maca polysaccharide might have a satisfactory effect on life span and anti-oxidant.关键词：Maca polysaccharide;Drosophila melanogaster;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Jiawei Duhuo Jisheng decoction in collagen-induced arthritis (CIA) rats. Method: Ninety male SPF Sprague-Dawley rats were randomly divided into the model control group, the leflunomide group (ig, 10 mL·kg-1), and the low-, medium-, high-dosage groups of Jiawei Duhuo Jisheng decoction (ig, 11, 22, 44 g·kg-1) of 6 rats each group. The CIA model was established except rats in the model control group by injection of 0.2 mg complete Freund's adjuvant at the end of the tail of rats. The rats in normal model group were given the same dosage of distilled water. All rats received oral administration of corresponding medicines once daily for 12 weeks. The body weight, arthritis index (AI), paw index, joint pathological changes of rats were observed at 0, 2, 4, 6, 8, 10 and 12 weeks after treatment. Result: The AI of rats in the low-, medium-, high-dosage Jiawei Duhuo Jisheng decoction groups and the leflunomide group have significant improvement, there was statistically significant difference as compared with that in the model control group (P<0.05, P<0.01). The body weight, paw index changes were significantly improved after treatment than the model control group (P<0.05, P<0.01). The ankle joint cartilage pathological had an improvement in the high-dosage Jiawei Duhuo Jisheng decoction groups as compared with the control model group. Conclusion: Jiawei Duhuo Jisheng decoction can significantly improve the joint arthritis of CIA rats, which is an effective prescription used for treating rheumatoid arthritis.关键词：arthritis;rheumatoid;Jiawei Duhuo Jisheng decoction
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Xiaozhi decoction on sterol regulatory element-blinding protrin-1c (SREBP-1c) mRNA and SREBP-1c protein expression levels in hepatic tissue of rats with non-alcoholic fatty liver disease(NAFLD) and to explore the molecular mechanism. Method: NAFLD model was established by feeding high fat diet for 8 weeds. Then model rats were randomly divided into 5 groups:model group,fenofibrate group,Xiaozhi decoction group(high-dose group,dose group,low-dose group),and additional normal control group. All rats were given normal diet. Meanwhile, rats in medication groups were fed with corresponding herbs, others were fed with corresponding NS. Four weeks after the treatment, all rats were anesthetized by 3% pentobarbital sodium through intraperitoneal injection. total cholesterol(TC), triglyceride(TG) content in liver tissue and TC, TG, glutamine transpeptidase(GGT), aspartase aminotransferase(AST), alanine aminotransferase(ALT), content in the serum was detected by automatic biochemical analyzer. The pathological changes of hepatic tissue were observed by HE staining.The sterol regulatory element-blinding protein-1c (SREBP-1c)mRNA expression levels in liver tissue was examined by reverse transcription polymerase chain reaction(RT-PCR). The SREBP-1c protein expression levels in hepatic tissue was examined by western blot. Result: Compared with normal group, blood lipid, liver fat, free fatty acide(FFA), insulin (INS), insulin resistance index(HOMAR-IR), malondialdehyde(MDA), the SREBP-1c mRNA and protein expression was significantly increased in model group (P<0.05 or P<0.01);superoxide dismutase(SOD) content decreased significantly (P<0.01).Compared with the model group, the hepatic Steatosis degree was alleviated obviously in the Xiaozhi decoction-high-dose group. TC, TG, AST, ALT, GGT content in serum, and TC,TG content in liver tissue were dramatically declined(P<0.05 or P<0.01). The SREBP-1c mRNA and protein in treatment groups was down-regulated (P<0.01),especially in the Xiaozhi decoction-high-dose group, whose expression was much lower compared with model group(P<0.01). Conclusion: Xiaozhi decoction showed certain therapeutic effect on NAFLD, which might be linked to down-regulating SREBP-1c mRNA and protein expression.关键词：non-alcoholic fatty liver disease;Xiaozhi decoction;sterol regulatory element-blinding protrin-1c
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发布时间：2024-01-04 - 摘要：Objective: To investigate the potential antitumour effects of polyphyllin Ⅰ(PPⅠ) on urothelial carcinoma of the bladder. Method: Urothelial carcinoma of bladder cell lines BIU-87 were cultured with or without PPⅠ. Cell proliferation were measured by MTT assay. Cell apoptosis and cell cycle were measured by flow cytometry. The changes of cell morphology were observed by transmission electron microscopy. The expression of Bcl-2 was detected by real-time PCR and western blot. Result: The results showed that the proliferation of BIU-87 cells were significantly inhibited by PPⅠ. The 50% inhibitory concentrations (IC50) of PPⅠ were 9.71, 5.91, 4.68 μmol·L-1 at 24,48,72 h. The inhibition rate of PPⅠwas in a manner of dose- and time-dependent. The treatment of PPⅠ led to activation of apoptosis. After being treated with PPⅠ, the apoptotic rate of BIU-87 cells was remarkably higher than that of the control. The results of real-time PCR and western blot showed that the expression of Bcl-2 was decreased in the group of PPⅠ treated. Conclusion: These results suggest that PP Ⅰpotentially has anticancer effects on urothelial carcinoma of the bladder cells in vitro. To deregulate the expression of Bcl-2 is one of the potential mechanisms.关键词：urothelial carcinoma of bladder;polyphyllin Ⅰ;proliferation;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Sophorae Tonkinensis Radix et Rhizoma (STR) on the activity of cytochrome P450 (CYP450) in rats. Method: Wistar rats were randomly divided into normal control group and STR low dose (1 g·kg-1) and high dose (12 g·kg-1) groups. STR 1, 12 g·kg-1 groups were treated with STR decoction by intragastrical administration,while the normal control group was given distilledwater of the same volume once a day for 21 consecutive days. The blood was collected after 21 day treatment. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin (TB) were measured by using automatic biochemical analyzer. The liver weight index was calculated and the pathological changes of hepatic tissues were observed. The activity of the subtype enzymes of CYP450 including CYP1A2, CYP2E1, CYP3A1, CYP2C9 and CYP2D6 of the liver was measured by probe drugs. Result: Compared with the normal control group, the STR high dose group demonstrated certain liver toxicity, including the increased activity of ALT, ALP and liver weight index (P<0.01), spotty necrosis and inflammatory cell infiltration in the liver of some animals. The high dose of STR induced the activity of CYP3A4, CYP2C9 and CYP2D6 (P<0.05 or P<0.01). Compared with low dose of STR, high dose of STR induced more activity of CYP3A4 (P<0.01). Conclusion: High dose of STR can induce the activity of CYP3A4, CYP2C9 and CYP2D6, and it is more sensitive to CYP3A4, suggesting that it should pay more attention to the drug interactions when STR is used in the combination with other drugs which also have the effects on CYP3A4.关键词：Sophorae Tonkinensis Radix et Rhizoma;liver injury;CYP450
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发布时间：2024-01-04 - 摘要：Objective: To investigate Shenqi Fuzheng injection in combination with interferon-α treatment of advanced primary liver cancer clinical efficacy and adverse reactions. Method: A retrospective case studies selected has lost surgery, radiotherapy and chemotherapy alone in patients with advanced hepatocellular carcinoma and combined interferon-α and interferon-α Shenqi Fuzheng injection rate of tumor regression in patients, survival, Karnofsky functional status score(KPS), weight changes and side effects of treatment during the study comparing the treatment of poisoning. Result: The combination group in tumor shrinkage rate, survival, KPS score, weight changes during treatment and side effects of treatment of poisoning are better than interferon-α alone group. Conclusion: Shenqi Fuzheng injection combination with interferon-α play a synergistic role in the treatment of advanced primary liver cancer when, there is a unique effect in prolong survival and improve quality of life for patients, the adverse reactions were mild and worth clinical application.关键词：middle-late stage primary liver cancer;interferon-α;Shenqi Fuzheng injection
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this investigation was to study the effect of postoperative application of Brucea Javanica oil injection adjuvant chemotherapy on treating gastric cancer. Method: One hundred and eight patients with advanced gastric cancer were divided into observation group and control group. The control group was given XELOX adjuvant chemotherapy (oral capecitabine plus oxaliplatin into intravenous drip), while the observation group was given auxiliary Brucea Javanica oil injection besides XELOX chemotherapy (oral capecitabine plus oxaliplatin into intravenous drip+Brucea Javanica oil injection intravenous drip). The long-term prognosis, adverse reactions, overall life quality and immune function were compared. Result: Complete response(CR)(8.62%), efficiency(58.62%), benefit rate(79.31%), karnofsky(KPS) score(84.37±8.21), QLQ-C30 score(81.84±8.34), CD3+, CD4+, CD8+, CD4+/CD8+, natural killer(NK)(9.31±1.34)%, tumor necrosis factor(TNF-α) (1.53±0.21) ng·L-1 of observation group were higher than control group, while local recurrence(10.34%), distant metastasis(10.34%), nausea and vomiting(10.34%), neurotoxicity(22.41%),liver function damage(10.34%), renal function damage (12.07%) were lower than control group. Conclusion: Postoperative application of Brucea Javanica oil injection adjuvant chemotherapy has positive therapeutic value for it can improve the prognosis and reduce the adverse reaction.关键词：gastric cancer;Brucea Javanica oil injection;adjuvant chemotherapy;prognosis
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this research was to study clinical efficacy of Buyang Huanwu decoction in treating cerebral thrombosis, and to provide a reference basis for clinical work in the future. Method: One hundred patients with cerebral thrombosis admitted were divided into the control group and the observation group according to different treatment methods. The patients in the control group were subject to conventional western medicine treatment, and the patients in the observation group were subject to the western medicine treatment combined with Chinese medicine treatment (Buyang Huanwu decoction treatment on the basis of the treatment applied on patients in the control group), then clinical efficacy and improvements of life quality of patients in two groups were observed. Result: As for the total effective rate of patients in two groups (P<0.05), the basic self-care of life after treatment (P<0.01), the seriously unable self-care of the life (P<0.01), the hematokrit of patients in two groups after treatment (P<0.01), the whole blood viscosity (P<0.01), and the fibrinogen (P<0.01),all the differences were statistically significant;and for the assistance of life needed, there was no significant difference. Conclusion: The Buyang Huanwu decoction delivers significant effect and high safety on treating cerebral thrombosis, and no serious adverse reaction was detected, which is worthy of clinical promotion.关键词：Buyang Huanwu Decoction;western medicine;conventional treatment;cerebral thrombosis
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this paper is to observe the clinical effect of taking Weiwei Tongtiao tang-a prescription from herbal medicine in Guizhou province and combining with injection into acupuncture point on treating chronic atrophic gastritis(CAG). Method: Randomly 120 cases which meet the diagnostic criteria were divided into 4 groups to cureby 4 different methods, the 1st method was only to take Weiwei Tongtiao tang (n=30), the 2nd was only to choose acupuncture point injection (n=30), the 3rd was to combine taking Weiwei Tongtiao tang and injection into acupuncture point(n=30), while the 4th was only to take Weifuchun tablet (n=30). After 3 months treatment, the score changes of clinical symptom and pathological signs before and treatment were compared. Result: We discovered that there was obvious improvement in the 3rd group comparing to others (P<0.05), and the expression of cyclooxygenase-2(COX-2) and Ki-67 of each group also improved evidently (P<0.05). Conclusion: It was suggested that Weiwei Tongtiao tang combining with acupuncture injection can obviously improve clinical symptom of CAG.关键词：Weiwei Tongtiao tang;acupuncture point injection;chronic atrophic gastritis;cyclooxygenase-2;Ki-67
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to compare the acaricidal effect of 5% tea tree oil gel and 2% arilin eye drops on the ocular Demodex folliculorum. Method: Eyelash samples were epilated from out-patient of ophthalmology to collect ocular D. folliculorum, transferred into 6-well plates and examined under the inverted microscope. Each well contained at least 20 live mites which were collected and treated with 5% tea tree oil gel and 2% arilin eye drops, respectively, with normal saline as blank control. Result: 5% tea tree oil gel was demonstrated to show better killing effect on the mite in vitro. After drug treatment, the morphology and activity of D. folliculorum altered with typical changes of excitation-spasm-chalasia-death. In the treatment of 5% tea tree oil gel, the average survival time of mites was (1.43±0.62)h, while in 2% arilin eye treatment, it was (27.65±5.14) h, with a significant difference between them(P<0.01).The survial times of mites, showed significant difference among the three groups(P<0.01). The survival time of D. folliculorum in normal saline group was (23.33±3.98) h, which had significant difference from the 5% tea tree oil gel group(P<0.01);while in the 2% arilin eye drops group the survival time was (27.65±5.14) h, compared with the survival time of control group, there was no significant difference between them. The mortality of mites within 24 hours in 5% tea tree oil gel, 2% arilin eye drops and control group were 100%, 20% and 50% respectively, and there was a significant difference between them(P<0.01). Conclusion: 2% arilin eye drops has no effect on killing D. folliculorum in vitro, while 5% tea tree oil gel has the killing effect on D. folliculorum in vitro. Tea tree oil gel could be developed as a natural drug to kill ocular D. folliculorum in high-efficency.关键词：tea tree oil ocular gel;arilin eye drops;Demodex folliculorum;survival time;mortality within 24 hours
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发布时间：2024-01-04 - 摘要：Objective: Curative effect of Bushen Jiangu decoction on primary osteoporosis (POP) and influence on bone mineral density, patients' qualities of lives and biochemical index of bone metabolism were investigated. Method: One hundred patients with POP were randomly divided into control group and observation group (50 cases) according to the order of diagnosis. Patients in both groups received non-drug guidance and oral administration of calcium carbonate-D3 chewable tablets, 0.6 g per day. Patients in control group took Xianling Gubao capsules orally, 3 grains each time, twice a day. Patients in observation group received modified Bushen Jiangu decoction, 1 dose a day,and took twice daily. Course of treatment in two groups were both for 6 months. Bone mineral density of neck of femur and 2nd and 4th lumbar vertebra (L2-4) were measured by dual energy X ray absorption meter before and after treatment, and levels of serum calcium (Ca), serum phosphorus (P), alkaline phosphatase (ALP) and bone gla protein (BGP) were detected. Qualities of lives were evaluated by determination of the scale of World Health Organization Qualities of Life-Brief(WHOQOL-BRIEF). Main symptoms and signs were graded before treatment and at the 2nd, 4th and 6th mouth after treatment. Occurrence of fracture was recorded. Result: Bone mineral density of neck of femur and lumbar vertebra (L2-4) in observation group were higher than those in control group (P<0.01) after treatment. Levels of Ca and BGP in observation group were higher than those in control group (P<0.01), however level of ALP was lower than that in control group. Total scores of qualities of lives, state of health, overall subjective sensation, physiology, mentality and social relations in observation group were higher than those in control group(P<0.01). Scores of main symptoms and signs in observation group were all lower than those in control group at the 2nd, 4th and 6th mouth after treatment (P<0.01). The total effective rate of treating osteoporosis in observation group was 94%, which is superior to that in control group (78%) (P<0.05). Rates of occurrence of fracture in both groups were lower, but there was no significant difference between two groups. Conclusion: Modified Bushen Jiangu decoction can improve bone mineral density of patients with POP, promote bone formation, restrain bone resorption, relieve clinical symptoms and improve qualities of lives.关键词：primary osteoporosis;Bushen Jiangu decoction;bone mineral density;qualities of life
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发布时间：2024-01-04 - 摘要：Objective: Observe the eradication rate of helicobacter pylori(Hp) of peptic ulcer(PU)and ulcer recurrence in patients treated with Qingyou 1st decoction combined with quadruple therapy. Method: Eighty-eight patients were randomly divided into control group and observation group (44 cases,respectively). Patients in control group received oral administration of omeprazole(20 mg each time), bismuth potassium citrate tablets(2 pieces each time)amoxicillin capsule(1 g each time)and clarithromycin(500 mg each time) twice a day. After 14 days of treatment Famotidine would continue to be used for at a dosage of 20 mg each time (once a day) for another 14 days. Based on the treatment of control group, patients in observation group added decoct medicinal herbs-Qingyou 1st decoction, (1 does per day, taken at two times) for another 28 days. Clearance of Hp was recorded after treatment. Quality of ulcer healing was evaluated with histologic regenerative mucosal maturity. One-year follow-up was made to observe ulcer recurrence. Result: The total curative efficacy in observation group was 97.73%, which is higher than that in control group (81.82%), the difference was statistically significant (P<0.05). Hp clearance rate in observation group was 90.9%, which is higher than that in control group (75%, P<0.05). Histological maturity of regenerative mucosa in observation group was superior to that in control group (P<0.05). Number of cases of ulcer recurrence in control group was 13, and rate of recurrence was 45.16%, while number of ulcer recurrencee in observation group were 8, rate of ulcer recurrence was 20%, which in observation group was lower than that in control group(P<0.05). The average time of ulcer recurrence in observation group(10.1±1.93)months were longer than which in control group(8.7±1.84)months (P<0.05). Conclusion: On basis of routine quadruple therapy, added Qingyou 1st decoction can improve Hp clearance rate and the quality of ulcer healing. The combined method is able to reduce/delay PU recurrence and enhance the clinical curative effect.关键词：peptic ulcer;helicobacter pylori;quadruple therapy;Qingyou 1st decoction;helicobacter pylori clearance;ulcer healing quality
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发布时间：2024-01-04 - 摘要：Objective: Curative effect and mechanism of Tiaojing decoction on polycystic ovary syndrome(PCOS) were investigated. Method: Eighty-four patients with PCOS were randomly divided into control groupand Tiaojing decoction group (42 cases,respectively) by random number table. Patients in control group received ethinylestradiol and cyproterone acetate tablets, clomifene citrate and menotrophin injection. Based on the treatment of control group, Tiaojing decoction was added in Tiaojing decoction group. Course of treatment in two groups were 3 menstrual cycles. Condition of follicular development was monitored by B-ultrasonic wave and the blood flow of follicularis membrane was also detected, pulsatility index (PI) and resistance index (RI) were recorded. And levels of human chorionic gonadotropin(HCG),luteinizing hormone(LH) (injection day), serum testosterone(T), estradiol(E2) and follicle-stimulating hormone(FSH) were detected. Levels of fasting blood-glucose(FBG), fasting insulin(FINS) and leptin(LP) were detected, and homeostasis model assessment of insulin resistance(HOMA-IR) was calculated before and after treatment. Rates of menstruation recovery, ovulation and pregnancy were recorded. Result: Rates of menstruation recovery, ovulation and pregnancy in Tiaojing decoction group were 92.86%, 83.33% and 57.14% respectively, which were superior to those in control group(73.81%,61.90% and 35.71%) (P<0.05). Compared with that before treatment, PI in both groups increased, and which in Tiaojing decoction group was higher than in control group (P<0.01). Compared with that before treatment, RI in both groups decreased, and which in Tiaojing decoction group was lower than in control group (P<0.01). Levels of FSH, LH and E2 in Tiaojing decoction group were higher than those in control group (P<0.01), and level of serous testosterone was lower than in control group (P<0.01). After treatment, compared with those before treatment, FINS, LP and IR in both groups decreased, and which in Tiaojing decoction group were lower than those in control group(P<0.05). Conclusion: Based on the routine western medicine treatment, Tiaojing decoction can ameliorate blood flow ovary, improve quality of follicle, ameliorate hyperandrogenism and insulin resistance and improve rates of ovulation and pregnancy.关键词：polycystic ovary syndrome;infertility;quality of follicle;hormone secretion;leptin;Tiaojing decoction
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发布时间：2024-01-04 - 摘要：Objective: Short-term and long-term effects of modified Hewei Jiangni decoction in treating Gastro-esophageal reflux disease(GERD)(stagnancy heat of the liver and stomach type) and to quality of lives were investigated. Method: One hundred and twenty-eight patients with GERD were randomly divided into western medicine groupand observation group (64 cases,respectively). Patients in western medicine group received Omeprazole Magnesium enteric-coated tablets, 20 mg each time, taken at the morning and evening. Mosapride citrate tablets, 5 mg each time, three times per day. Patients in observation group received Omeprazole Magnesium enteric-coated tablets and the dose was same as which in western medicine group, and also received treatment of modified Hewei Jiangni decoction,1 dose per day. Course of treatment in both groups was 8 weeks. Scores of symptoms of stagnancy heat of the liver and stomach, endoscopic of esophageal mucosa, reflux diagnostic questionnaire(RDQ) and life quality were evaluated before and after treatment. Recovered patients received a 6-moth follow-up, recurrence rates and scores of endoscopic of esophageal mucosa were graded. Result: The total effective rate of improvement of traditional Chinese medicine syndrome in observation group was 93.75%, which is superior to that in western medicine group(81.25%)(P<0.05). The total effective rate of elimination of inflammation in observation group was 90.62%, which is superior to that in western medicine group(75%)(P<0.05). Scores symptoms of stagnancy heat of the liver and stomach, endoscopic of esophageal mucosa and RDQ in observation group were all lower than those in western medicine group (P<0.01). Except for score of physical pain, the SF36 scores of health scale in observation group were higher than those in western medicine group (P<0.01). During the 6-mouth follow-up, recurrence rate in observation group was 23.5%, which is lower than that in western medicine group(64%), and when it relapse, score of endoscopic of esophageal mucosa in observation group was lower than that in western medicine group(P<0.01). Conclusion: Based on the treatment of proton pump inhibitor, treatment of modified Hewei Jiangni decoction not only relieve short-term symptoms of GERD(stagnancy heat of the liver and stomach type), but also improve cure rate and quality of lives, furthermore reduce long-term recurrence rate.关键词：gastroesophageal reflux disease;Hewei Jiangni decoction;stagnated heat of liver and stomach type;recurrence rate;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To observe short-term curative effect of Qihong Jieyu decoction and changes of N-terminal pro-brain natriuretic peptide (NT-proBNP) on patients with depression secondary to chronic heart failure. Method: One hundred and twenty patients with depression secondary to chronic heart failure were randomly divided into control group and observation group (60 cases,respectively) according to order of hospitalization. Patients in both groups' receive routine western medicine treatment of congestive heart failure. Patients in control group received paroxetine. Patients in observation group received Qihong Jieyu decoction,1 dose per day. Treatment in both groups continued to 8 weeks and a 6-month follow-up was made at the end of treatment. Scores of Clinical Lee's heart failure, Hamilton's depression scale(HAMD), self-rating depression scale(SDS) and Minnesota heart failure quality of life questionnaire(MLHFQ) were made before and after treatment. Left ventricular ejection fraction(LVEF) and cardiac output(CO) were measured. Condition of re-hospitalization was recorded during the 6-month follow-up. And level of NT-proBNP was also detected. Adverse reaction were also recorded during the observation period. Result: The total curative efficacy of Clinical Lee's heart failure in observation group was 86.67% and that in control group was 71.67%, the difference was significant (P<0.05). And the total curative efficacy of clinical depression in observation group was 88.33%, higher than that in control group 81.67%, however there was no significant differences between two groups. Scores of Lee's heart failure, HAMD, SDS and MLHFQ in observation group were lower than those in control group (P<0.01). LVEF and CO in observation group were higher than those in control group (P<0.01). Level of NT-proBNP was lower than that in control group (P<0.01).Occurrence rate of adverse reaction in control group was 58.3%, and there was no adverse reaction in observation group. During the 6 mouths' follow-up visit, rate of re-hospitalization in observation group was 36.66%, which is lower than 61.66% in control group (P<0.05). Conclusion: Qihong Jieyu decoction can significantly ameliorate symptoms of heart failure and depression of patients with depression secondary to chronic heart failure, improve patients' qualities of lives, lower rate of re-hospitalization, and there is a definite curative effects and lower adverse reaction.关键词：chronic heart failure;depression;Qihong Jieyu decoction;quality of life;N-terminal pro-brain natriuretic peptide
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发布时间：2024-01-04 - 摘要：Objective: To evaluate Tongluo Qianzheng decoction improvement effects of acupuncture combined with medicine on nerve function defect and the quality of life in stroke patients during rehabilitation. Method: One hundred and twenty-four stroke patients during rehabilitation were randomly divided into control group and observation group (62 cases,respectively) by random number table. Patients in control group received Bobath method, mandatory exercise therapy, body weight support task-specific training, motor relearning program, etc.. Based on the treatment of control group patients, patients in observation group added acupuncture treatment and oral Tongluo Qianzheng decoction. The patients in both groups received 8-weeks periods of treatment. Score the US national institutes of health stroke scale (NIHSS) was graded before and after treatment. Activities of daily living (ADL) was evaluated through Barthel index. Spasm degree was estimated using Ashworth method. Body comprehensive movement function was assessed by Adopt Fugl-Meyer motor function assessment scale and quality of life was evaluated by functional comprehensive assessment (FCA) scale. Besides, Chinese medical syndrome was graded before and after treatment. Result: The total curative rate in observation group was 93.5%, which is superior to that in control group 77.4%(P<0.05). The scores of NIHSS, Ashworth, Fugl-Meyer (Upper limb and lower limb) in observation group were lower than in control group, while the Barthel index in observation group was higher than that in control group (P<0.01) after treatment. FCA scores in both groups increased, which was higher in observation group than in control group (P<0.01) after treatment. Score of Chinese medical syndrome in two groups decreased, which was lower in observation group than in control group (P< 0.01). Amelioration degree of motor function damage of limbs and classification of Ashworth muscular tension in observation group was superior to that in control group (P<0.05). Conclusion: Rehabilitation therapy of acupuncture combined with medicine can ameliorate spasticity and nervous functional defects,and it can also relieve clinical symptoms and signs, and furthermore it can improve ADL and quality of live in stroke patients. Therefore, acupuncture combined with medicine therapy could play an important role in the system of modern rehabilitation treatment by integrated Chinese and western medicine.关键词：stroke;convalescence;neurologic impairment;acupuncture and moxibustion;Tongluo Qianzheng decoction
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发布时间：2024-01-04 - 摘要：Objective: This study aimed to analyze the relationship between the traditional Chinese medicine (TCM) formula with different proportions and the treatment for diseases by using the method of statistical analysis. Method: Totally 1 557 TCM formulae of rhizoma atractylodis macrocephalae and radices paeoniae alba were retrieved from the ‘Traditional Chinese Medicine Formula Database’,and then a new database was built to analyze the relationship among the ratio,the corresponding cured disease and the department. Result: The ratio of 1:1 appeared to be the most(734 times,47.1%) in the 1 557 formulae containing rhizoma atractylodis macrocephalae and radices paeoniae alba, which was most frequently used for the treatment of the diseases in internal medicines (717 times, 46.1%) and gynecology(581 times, 37.3%). Conclusion: Using the method of statistical frequency analysis can effectively elucidate the relationship between rhizoma atractylodis macrocephalae and radices paeoniae alba, which can effectively discover the application pattern of the TCM formulae, to provide a theoretical basis for clinical application, also to provide a reference for TCM formulae research as well as new drug research and development.关键词：Rhizoma Atractylodis Macrocephalae;Radices Paeoniae Alba;herbal pair;ratio;statistical analysis
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发布时间：2024-01-04 - 摘要：Objective: This study was to assess the efficacy and safety of Xiyanping plus azithromycin in curing the mycoplasma pneumonia. Method: Some Chinese-english databases including Pubmed,Embase,CENTRAL,CBM,CNKI,VIP and wanfang were searched to collect the Randomized controlled trials (RCTs). After two reviewers have selected trials independently and assessed methodologically quality by Jadad scale, valid data would be extracted to make a Meta-analysis. Result: A total of 11 RCTs were brought in, including 942 cases. Meta-analysis showed that, the treatment group of Xiyanping combined with azithromycin has advantages on the total effective rate [OR=4.08,95%CI(2.14,7.78)], cooling time [MD=-1.17, 95%CI(-1.49,-0.85)], cough-disappear time [MD=-2.04,95%CI(-2.63,-1.44)], healing time [MD=-2.60,95%CI(-3.02,-2.16)], rales disappeared time [MD=-1.52,95%CI(-2.81,-0.22)] and the reduction of occurrence of adverse reactions [OR=0.48,95%CI(0.30,0.77)]. However, both of the groups didnt show significant difference on X-ray-disappear time [MD=-7.55,95%CI(-17.80,2.70)]. Conclusion: We concluded that the efficacy of treatment group which combine Xiyanping with azithromycin is superior to azithromycin alone.关键词：Xiyanping;azithromycin;mycoplasma pneumonia;Meta-analysis
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发布时间：2024-01-04 - 摘要：Through investigation and analysis for approval of tablets health foods,to investigate its frequency of usage of raw materials,extracts of raw materials and auxiliary materials,summarize problems existed in registration,alteration and evaluation of tablets health foods in recent years,in order to provide data reference for research and development,supervision,reporting of tablets health foods.Taking database of center for health food evaluation of state food and drug administration as data source,statistical summary(6 560 pieces) of registration and approval of health foods during 2005—2013,then involving tablets health foods(1 755 pieces) were analyzed,which domestic products accounted for 97.6% of the total number.Main nutriment claims is added vitamins and minerals,their main function claim is enhance immunity,main raw materials are calcium carbonate and vitamin C.Despite tablets have many advantages,but not all of raw materials are suitable for making into this formulation.关键词：tablets;health food;review;approval;regulation
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发布时间：2024-01-04 - 摘要：Through the analysis of the related research literatures on Aconiti Lateralis Radix Praeparata, we found that its clinical adverse reactions are associated with its toxic components, overdosing, decoction, drug compatibility, processing, methods of administration, misdiagnosis, misuse, individual differences and some other factors. Much attention should be paid to the factors mentioned above to avoid its clinical adverse reactions, that is to say, we should give high considerations on three aspects:clinicians, drugs and patients. This paper aims to discuss the characteristics and causes of clinical adverse reactions due to Aconiti Lateralis Radix Praeparata, so as to provide references for clinical medication safety.关键词：Aconiti Lateralis Radix Praeparata;adverse reactions;drug compatibility;processing
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发布时间：2024-01-04 - 摘要：The principle and characteristics of ultrasonic extraction of chemical constituents in traditional Chinese medicine were introduced in this paper. It also summarized the research progress in ultrasonic extraction of effective components in plant medicine, animal medicine and fungus medicine. The defect and deficiency in the process of ultrasonic extraction were pointed out and the research direction of ultrasonic extraction was also prospected. This paper will provide some reference for researchers in extracting the chemical composition of traditional Chinese medicine using ultrasonic technology.关键词：ultrasonic extraction;traditional Chinese medicine;chemical composition
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发布时间：2024-01-04 - 摘要：Objective: Dioscorea Panthaica Rhizoma is the main raw material of Diao Xinxuekang, which was approved to sell as drug in Holland in 2012.In this review, researches of the chemical components of D. panthaica, their pharmacological actions and clinical applications were summarized based on the domestic and abroad literatures in past 15 years. The researches of the chemical constituents in D.panthaica focused on steroid saponins, which had curing myocardial ischemia, hypolipidemic and antitumous effecst, and its treatment of myocardial ischemia had drawn widely attention of medical field. Compounds isolated from this herb were less, and only dioscin underwent further researches of pharmacological activities. While the discoveries of the other steroidal saponins and their chemical constituents as well as their pharmacological actions were very scarce. Thereby further research of D. panthaica is worthwhile to fulfill the use of plant resources.关键词：Dioscorea Panthaica Rhizoma;chemical components;pharmacological actions;clinical applications
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