最新刊期
卷 20 ， 期 17 ， 2014
- 摘要：Objective: To optimize formulation technology of betulinic acid ethosomes and investigate feasibility of ethosomes as a transdermal carrier for betulinic acid. Method: Betulinic acid ethosomes were prepared by ethanol injection method,taking entrapment efficiency as index,an orthogonal design was used to optimize formulation process of betulinic acid ethosomes with ratio of betulinic acid to phospholipid,phosphate buffer pH and ethanol concentration as factors.Shape and particle size of betulinic acid ethosomes were analyzed by transmission electron microscopy and laser particle size analyzer.In vitro transdermal delivery characteristics of betulinic acid were evaluated by using Franz diffusion cells,and compared with liposomes and 10% isopropanol solution of betulinic acid.HPLC was employed to determine the content of betulinic acid with mobile phase of acetonitrile-0.1% glacial acetic acid(70:30) and detection wavelength at 207 nm. Result: Optimal formulation process parameters for betulinic acid ethosomes were soybean lecithin-betulinic acid(19:1),pH of phosphate buffer 7.4 and ethanol concentration of 30%.Average particle size of ethosomes under these conditions was 94.3 nm,encapsulation efficiency was 86.3%.Accumulative permeation amount of betulinic acid ethosomes within 12 h was (108.33±10.26)μg·cm-2,which was about 2.12 times of liposomes and 5.98 times of 10% isopropanol solution. Conclusion: Ethosomes,as a vector for transdermal drug delivery,could significantly promote transdermal penetration of betulinic acid better than liposomes.This study provided new ideas for anti-tumor and anti-acquired immunodeficiency syndrome treatment of pentacyclic triterpenoids.关键词：betulinic acid;ethosomes;liposomes;transdermal delivery system;in vitro transdermal test
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发布时间：2024-01-04 - 摘要：Objective: To investigate adsorption kinetics and thermodynamic characteristics of macroporous resin for total triterpenoids from Schizandrae Sphenantherae Fructus,in order to provide a reference for separation and purification of these compounds. Method: With adsorption and elution rate as indexes,optimum type of macroporous resin for purification of total triterpenoids was selected from 11 kinds of macroporous resin by static adsorption and desorption test,then adsorption kinetics model and adsorption isotherm model of total triterpenoids were established by AB-8 resin and calculated thermodynamic parameters. Result: AB-8 resin was selected,resin adsorption processor for total triterpenoids showed fast adsorption stage in 0-50 minutes and slow adsorption stage in 50-200 minutes,adsorption after 200 minutes was saturation stage.Adsorption dynamic behavior was well described by quasi-second-order kinetics equation,adsorption rate was mainly controlled by liquid film diffusion and intraparticle diffusion;equilibrium adsorption data were fitted Freundlich isotherm equation which was belonging to multi-molecular layer adsorption;high temperature was advantageous to adsorption. Conclusion: Adsorption process of AB-8 resin for total triterpenoids was driven by entropy,solid-liquid interface molecular motion was more confusion,more water molecules promiscuously moved from solid surface to solution,which had a certain guiding significance for optimizing purification process of total triterpenoids from Schizandrae Sphenantherae Fructus.关键词：Schisandrae Sphenantherae Fructus;total triterpenoids;macroporous resin;adsorption kinetics;adsorption thermodynamics
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Hirudo Zhusan. Method: The content of antithrombin activity substance was determined by anti-thrombin titration.Taking grinding degree as index to screen smash equipment,then effect of water content on powder of Hirudo Zhusan was investigated;with extract yield and transfer rate of antithrombin activity substance as indexes,single factor tests were adopted to optimize extraction process by taking soaking time,the amount of water,decoction time and so on as factors,then compared with traditional pieces of Hirudo. Result: Optimum extraction parameters was as follows:soaked 10 min with 20 times the amount of water,decocted thrice at 100 ℃ for 20 min each time;transfer rate of antithrombin activity substance could reach as highly as 93.31%,which was significantly higher than traditional pieces of 52.44%. Conclusion: This optimized process was stable and feasible,Hirudo Zhusan had advantages of high efficiency,short time,low energy consumption,environmental protection and so on,it could provide an insight for industrial production and new drug development of Hirudo Zhusan.关键词：Hirudo Zhusan;anti-thrombin titration;hirudin;extract yield
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of volatile oil from Houttuyniae Herba dropping pills. Method: Volatile oil from Houttuyniae Herba was extracted by supercritical CO2 fluid extraction.Taking overall desirability of appearance grade,dissolve time limit and methyl-n-nonyl-ketone content as dependent variable,on the basis of single factor tests,central composite design and response surface methodology was adopted optimize preparation technology with dropping distance,proportion of matrix and volatile oil,liquid temperature and upper temperature of condenser pipe as independent variables.The content of methyl-n-nonyl-ketone was determined by GC. Result: Optimum preparation technology was as follows:dropper caliber of 2 mm,dripping speed of 50 d·min-1,matrix of PEG 4000-PEG 6000 (1:1),dimethyl silicone oil as condensate,dropping distance of 6.8 cm,proportion of matrix-volatile oil (3.7:1),liquid temperature at 87.4 ℃ and upper temperature of condenser pipe at 7.9 ℃;average values of appearance grade,dissolve time limit and methyl-n-nonyl-ketone content were 82 points,16.4 min and 1.83 μg·g-1,whose deviation were 2.04%,1.49% and 1.61% by comparing with these predicted values,respectively. Conclusion: Central composite design and response surface methodology was suitable for optimizing preparation process of this preparation with good predictability,this study could provide a reference for development of Houttuyniae Herba preparations.关键词：volatile oil from Houttuyniae Herba;dropping pills;methyl-n-nonyl-ketone;central composite design and response surface methodology;overall desirability
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different processing methods on concents of oligosaccharides and monotropein in Morindae Officinalis Radix. Method: Processed products of Morindae Officinalis Radix were prepared by different methods,contents of oligosaccharides and monotropein in different processed products were compared.The content of oligosaccharides was determined by HPLC-CAD with Shodex Asahipak NH2 column,mobile phase of acetonitrile(A)-water(B) for gradient elution (0-15 min,86%-81%A;15-20 min,81%-75%A;20-35 min,75%-70%A;35-50 min,70%-58%A;50-60 min,58%-40%A;60-65 min,40%-20%A);the content of monotropein was determined by HPLC-DAD with Venusil MP C18 column,mobile phase of methanol-0.4% phosphoric acid for gradient elution (5:95-28.8:71.2,15 min) and detection wavelength at 235 nm. Result: The content of oligosaccharides was in the order of Morindae Officinalis Radix steamed by salt >Morindae Officinalis Radix without the wood >Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma >crude Morindae Officinalis Radix;while according to the content of monotropein was Morindae Officinalis Radix steamed by salt >crude Morindae Officinalis Radix >Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma >Morindae Officinalis Radix without the wood. Conclusion: Processing could greatly change contents of oligosaccharides and monotropein in Morindae Officinalis Radix,which provided a reference for pieces quality control and processing mechanisms analysis of Morindae Officinalis Radix.关键词：processing technology;Morindae Officinalis Radix;oligosaccharides;monotropein
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of chlorogenic acid from Lepisorus ussuriensis. Method: On the basis of single factor tests,taking material-liquid ratio,ethanol concentration and extracting time as independent variables,yield of chlorogenic acid in response to a value,according to Box-Benhnken experiment design principle,extraction process of chlorogenic acid from L. ussuriensis was optimized by response surface analysis. Result: Optimum extraction process was as following:extracted twice with 68 times the amount of 75% ethanol for 41 min per time;extracting amount of chlorogenic acid was 8.26 mg·g-1,which was close to the theoretical prediction of 8.47 mg·g-1. Conclusion: Compared with traditional processes,alcohol extraction process had characteristics,such as high extraction efficiency,less energy consumption,low cost,et al.This optimized process was stable and feasible for providing a reference for industrial production of L. ussuriensis.关键词：Lepisorus ussuriensis;chlorogenic acid;Box-Benhnken Response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology of total polyphenols from Juglandis Semen kernel pellicles by macroporous resin. Method: With Juglandis Semen kernel pellicles as raw material,total polyphenols was extracted by ethanol refluxing method,static adsorption-elution test for total polyphenols were carried out to select proper macroporous resin,single factor tests were adopted to optimize purification process by taking the concentration of sample solution,adsorption flow rate,eluent dosage and so on as factors. Result: X-5 macroporous resin showed the best separation efficiency by comparing with other resins,its optimum purification technology was as follows:the concentration of sample solution 3.0 g·L-1 with pH of 5.0,adsorption rate of 3 BV·h-1,sample volume of 9.5 BV,eluted with 2 BV of 70% ethanol,collected eluent;Purity of total polyphenol was enhanced from 64.52% to 79.64% with transfer rate of 89.83% in extract. Conclusion: This optimized process was stable and feasible,X-5 resin was suitable for initial purification of total polyphenols from Juglandis Semen kernel pellicles.关键词：Juglandis Semen kernel pellicles;total polyphenols;Macroporous resin;gallic acid;static adsorption-elution test
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发布时间：2024-01-04 - 摘要：Objective: To optimize water-extraction process of asperosaponin Ⅵ from Dipsaci Radix. Method: With transfer rate of asperosaponin Ⅵ as index,orthogonal design was used to optimize extraction process by taking liquid-solid ratio,extracting time and times as factors.The content of asperosaponin Ⅵ was determined by HPLC,mobile phase was acetonitrile-water(30:70) and detection wavelength set at 212 nm. Result: Optimum process conditions were as follows:extracted twice with 15,10 times the amount of water,2 h for per time.Under these conditions,transfer rate of asperosaponin VI was (94.83±0.309)%. Conclusion: This optimized process was simple,stable and productive,which was suitable for industrial production of Dipsaci Radix.关键词：Dipsaci Radix;water extraction process;orthogonal test;asperosaponin Ⅵ
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发布时间：2024-01-04 - 摘要：Objective: To optimize water extraction and alcohol precipitation technology of hemorrhoid fumigants. Method: With composite score of the content of total alkaloids and dry extract yield as index,effects of the amount of water,extraction times and time on extraction technology was investigated by orthogonal test;with the content of total alkaloids as index,orthogonal test was adopted to investigate effects of relative density of extract,concentration and time of alcohol precipitation on alcohol precipitation technology.UV was employed to determine the content of total alkaloids with detection wavelength at 416 nm. Result: The best water extraction and alcohol precipitation technology was as following:decocted twice with 10 times the amount of water for 1.5 h per time,relative density of extract of 1.06 g·mL-1 at 25 ℃,alcohol precipitation concentration 60%,storage time of 12 h;the concentration of total alkaloids was 2.040 g·L-1. Conclusion: These optimized technologies were stable and feasible to provide a reference for standardized production of hemorrhoid fumigants.关键词：hemorrhoid fumigants;acid dye colorimetry;total alkaloids;Sophorae Flavescentis Radix;phellodendrine;oxymatrine
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发布时间：2024-01-04 - 摘要：Objective: To optimize water extracting technology of Taibi granules. Method: With dry extract yield,contents of polydatin and emodin as comprehensive evaluation indexes,L9(34) orthogonal test was adopted to optimize extraction process by taking the amount of water,extraction times and time as factors.Contents of polydatin and emodin were determined by RP-HPLC,mobile phases were acetonitrile-water(21:79) and methanol-0.1% phosphoric acid solution(77:23),detection wavelengths were 306,254 nm,respectively. Result: Optimum extraction process was as follows:refluxing extracted 3 times with 10-fold water,1 h for each time;average values of dry extract yield,mass fractions of polydatin and emodin were 25.720%,0.076% and 0.510%,respectively. Conclusion: Optimized extraction technology was stable,feasible and suitable for industrial production of Taibi granules.关键词：Taibi granules;Extracting Technology;overall desirability;polydatin;emodin
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发布时间：2024-01-04 - 摘要：Objective: To probe into feasibility of cutting process of fresh Menthae Haplocalycis Herba,in order to look for the best processing method by cutting. Method: GC chromatography was employed to determine the content of menthol with temperature program(initial temperature at 100 ℃,keeping 7 min;raised to 120 ℃ with 3.5 ℃·min-1,keeping 1 min;raised to 122 ℃ with 0.5 ℃·min-1,then raised to 220 ℃ with 35 ℃·min-1),flow rate of 1.3 mL·min-1,injection volume of 1 μL and split ratio of 5:1.Quantitative analysis of volatile oil from Menthae Haplocalycis Herba was made and influence of different cutting methods on quality of its pieces was evaluated. Result: The content of volatile oil produced by fresh cutting process increased by 14.95% compared with that produced by using traditional cutting process so that the content of menthol increased by 5.60%. Conclusion: This fresh cutting process for Menthae Haplocalycis Herba should be advocated so as to improve pieces quality and processing efficiency.关键词：Menthae Haplocalycis Herba;fresh cutting process;traditional cutting process;Menthol;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: To find out nanofiltration enrichment process of extract solution of Salviae Miltiorrhizae Radix et Rhizoma. Method: Taking salvianolic acid B as detection object,HPLC was employed to determine retention rate of salvianolic acid B with mobile phase of acetonitrile(A)-1% acetic acid(B) for gradient elution(0-20 min,5%-15%A;20-50 min,15%-40%A) and detection wavelength at 281 nm.With retention rate and membrane flux of salvianolic acid B as indexes,effects of operating pressure,adsorption characteristics and other factors on concentration process were investigated by single factor tests. Result: Retention rate of salvianolic acid B increased with decreasing of aperture and unchanged with change of pressure and cycles of concentratin.Membrane flux was positively correlated with intercept relative molecular weight and pressure,decreased as concentration ratio increased.Retention rate of salvianolic acid B was 99.5% when nanofiltration membranes aperture was 300 Da,membrane flux was 12 L·m-2·h-1,this process had no obvious influence to other ingredients in Salviae Miltiorrhizae Radix et Rhizoma with high concentration efficiency. Conclusion: Nanofiltration concentration is useful for concentrating liquid including thermal instability class ingredients,it has high efficiency and low losses of active ingredients.关键词：salvianolic acid B;nanofiltration process;Salviae Miltiorrhizae Radix et Rhizoma;membrane flux;retention rate
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for determining the content of solasonine in different extracts of Solanum torvum fruits. Method: Solasonine from S. torvum fruits was extracted by system solvent extraction method,contents of solasonine in different extracting parts were compared.The content of solasonine was determined by HPLC with mobile phase of acetonitrile-0.2% phosphoric acid aqueous solution(15:85),flow rate of 0.5 mL·min-1,detection wavelength at 250 nm and column temperature at 30 ℃. Result: Solanine showed a good linear relationship within 2-80 μg,average recovery was 100.93% with RSD of 0.60%.Elution parts of water,10% ethanol,30% ethanol,50% ethanol,70% ethanol,95% ethanol and extracting parts in petroleum ether,ethyl acetate,n-butanol,water were 0.062 9,0.939 0,0.020 5,0.118 0,0.005 1,0.004 4,0.010 6,0.031 4,0.070 5,0.113 0 mg·g-1,respectively. Conclusion: This method was simple,accurate and specific,it could be considered as an effective method for determinng the content of solasonine in different extracts of S. torvum fruits,which had quite difference in different extracting parts.关键词：Solanum torvum;different extracting sites;solasonine
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发布时间：2024-01-04 - 摘要：Objective: The study was aimed to develop a HPLC method for the determination of a new active component isoscutellarein in Hedyotidis diffusa. Method: The column was Kromasil-C18(4.6 mm×250 mm,5 μm). The acetonitrile-0.1% phosphoric acid(44:56) was used as mobile phase, with UV detection was at 280 nm,the detector drift tube and column temperature at 35 ℃. Result: The sample showed good linear relationship with sample volume in the range of 0.145-1.595 μg for isoscutellarein(r=1).The average recovery of isoscutellarein was 99.97%, with a RSD value of 0.95%. The content of isoscutellarein in leaf collected in August-September was the highest and was three to four times that of the stem. Conclusion: The method is simple,accurate,replicable,and can be used for the quality control of isoscutellarein in H. diffusa.The study also provides a theoretical basis for reasonable harvest time of H. diffusa.关键词：Hedyotidis diffusa;isoscutellarein;HPLC;harvest time;stem;leaf relationship with sample volume
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发布时间：2024-01-04 - 摘要：Objective: The study was performed optimize the HPLC conditions and to establish a method to determine content of six effective components in the ≤1 000 Da extract of Erhuang decoction (baicalin, baicalein, palmatine, berberine, liquiritin, glycyrrhizic acid) simultaneously. Method: The RP-HPLC method used for the determination, on a chromatographic column of Diamonsil, C18 column (4.6 mm×250 mm, 5 μm); with the was used mobile phase composed of acetonitrile:ammonium acetate solution (0.02 mol·L-1, acetic acid pH 4.0), in gradient elution;the flow rate was fixed at 0.5 mL·min-1;the detection wavelength was set at 280, 265, 250 nm;the column temperature:was maintained at 25 ℃;the injection volume was 20 μL. Result: There is a good linear relationship between the chromatographic peak area and concentration of baicalin, baicalein, palmatine, berberine, liquiritin, glycyrrhizic acid in the linear range, and the recovery are all in the range of 95%-105%, with all the RSD of the recovery rate less than 2%. Conclusion: The method is rapid and simple for the content determination of six components content determination in the ≤1 000 Da extract of Erhuang decoction.关键词：Erhuang Decoction;baicalin;baicalein;palmatine;berberine;liquiritin;glycyrrhizic acid
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发布时间：2024-01-04 - 摘要：Objective: The pupose of this article study the effects of gypsum on the contents of ephedrine, pseudoephedrine, amygdalin, liquiritin and glycyrrhizic acid in Maxingshigan decoction. Method: HPLC method was employed to determine the five components in both decoctions with and without gypsum. The Agilent ZORBAX SB-Aq C18 column (4.6 mm×250 mm, 5 μm) maintained at 30 ℃ with a gradient mobile phase system consisting of ACN-0.1% phosphoric acid solution was used for sample analysis. The wavelength of the UV detector was set at 207 nm for ephedrine, pseudoephedrine and amygdalin, while 237 nm for liquiritin and glycyrrhizic acid with a flow rate of 1.0 mL·min-1. Result: The concentration of ephedrine, pseudoephedrine, amygdalin, liquiritin and glycyrrhizic acid were 197.80, 64.32, 448.05, 56.81, 119.76 mg·L-1 in traditional Maxingshigan decoction with gypsum while in the case of gypsum-free, the concentrations of the five componts were 183.08, 54.57, 368.79, 50.34, 143.92 mg·L-1 in Maxingshigan decoction without gypsum, respectively. Conclusion: Gypsum had influence on the contents of other constituents. It could significantly increase the contents of ephedrine, pseudoephedrine, amygdalin, liquiritin but reduce the content of glycyrrhizic acid.关键词：Maxingshigan decoction;Gypsum;ephedrine;pseudoephedrine;amygdalin;liquiritin;glycyrrhizic acid
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发布时间：2024-01-04 - 摘要：Objective: The study was conducted to establish the method of degree incidence and to evaluate the safety of Angelicae Sinensis Radix from different habitats. Method: The contents of total ash, acid insoluble ash, Pb, Cd, As, Hg, and Cu in Angelicae Sinensis Radix were determined. The grey model was established by DPS V7.0.5 statistical software via the measurement of relative incidence degree. Result: The relative incidence degrees of all evaluation items were between 0.380 5-0.600 0; totally the relative incidence degrees from 25 batches of Angelicae Sinensis Radix were less than 0.500 0, which had better safety, including 9 batches from Min County, Gansu Province, 3 batches from Weiyuan County, Gansu Province, 3 batches from Zhang County, Gansu Province, and 4 batches from Yunnan Province, accounting for 90.0%, 50.0%, 60.0%, and 100% of the total number of their samples, respectively. The results based on the established model showed that the evaluation results were consistent with the genuineness meaning of Angelicae Sinensis Radix. Conclusion: This method and model can be well used to evaluate the safety of Angelicae Sinensis Radix.关键词：Angelicae Sinensis Radix;safety evaluation;grey incidence degree method
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发布时间：2024-01-04 - 摘要：Objective: This study was conduct to establish an UPLC method for the simultaneous determination of five components aesculin, esculetin, lactucin, cichoric acid and lactucopicrin in Uyghur drug Cichorium glandulosum and C. intybus. Method: Hypersil BDS C18 (4.6 mm×250 mm, 5 μm) was used as the stationary phase. The mobile phase was composed of acetonitrile and 0.4% phosphoric acid aqueous with a gradient elution. The flow rate was 1.0 mL·min-1. Column temperature was 30 ℃. The detection wavelength was 254 nm. Result: All calibration curves showed good linear regression (r>0.999 3) within test ranges. The validation data indicated excellent precision and good recovery. Conclusion: The method was successfully applied to quantify five components in C. glandulosum and C. intybus, which could be used as comprehensive quality evaluation of Cichorium.关键词：Cichorium glandulosum;C. intybus;aesculin;esculetin;lactucin;cichoric acid;lactucopicrin
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发布时间：2024-01-04 - 摘要：Objective: This study was performed to evaluate the quality of Euodiae Fructus from different varieties and locations and to provide the foundation for quality control it in the market by UPLC-UV fingerprint and content determination of active compounds of limonin, evodiamine and rutaecarpine. Method: The separation was performed on an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm) with gradient elution using acetonitrile-water asmobile phase. The flow rate was 0.5 mL·min-1. UV detecting wavelength was set at 210 nm, and column temperature was set at 25 ℃. The holistic quality of 24 samples were evaluated by fingerprint combined with unsupervised principal component analysis (PCA). The three active compounds in 24 samples collected from different varieties and locations were determined. Result: An ultra-performance liquid chromatographic method was established for fingerprint analysis, and nine peaks in UPLC profile were found as the same. The result of fingerprint similarity evaluation showed that the quality of Euodiae Fructus collected from Guizhou was stable and consistent, however, that from Zhejiang was inconsistent slightly. The result of PCA showed that there is difference in the components of Euodiae Fructus from different varieties. The contents of limonin, evodiamine and rutaecarpine in Euodiae Frutus were influenced by locations. Conclusion: The fingerprint analysis combined with the content determination of active compounds was available for holistic quality evaluation of Euodiae Fructus, which is directive is directive significance to origin identification and quality control of Euodiae Fructus.关键词：Euodiae Fructus;limonin;evodiamine;rutaecarpine;fingerprint;principal component analysis;UPLC
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发布时间：2024-01-04 - 摘要：Objective: This study was conducted to establish a systematic method for the qualitative and quantitative analysis of the major contents from Polygonati Odorati Rhizoma. Method: TLC method was used to identify the authenticity of Polygonati Odorati Rhizoma by TLC, while HPLC-LISD method was applied to determinate its saponins POD I by HPLC-ELSD. The TCL conditions included three developing systems:A consisted of petroleum ether, chloroform, methanol and formic acid with the ratio of 1:9:0.65:0.05, B consisted of chloroform, acetone and formic acid with the ratio of 7:1.1:0.05 and C consisted of cyclohexane, ethyl acetate, n-butanol and formic acid with the ratio of 5:2:0.3:0.03.Chromogenic agents were composed of 1% potassium ferricyanide and 1% mixing ferric chloride ethanol solution(1:1). Chromatographic separation was carried out on an Agilent ZORBAX SB-C18chromatographic column (4.6 mm×250 mm, 5 μm). The chromatographic conditions were as follows:flow rate of 1.0 mL·min-1;sample injection volume of 10 μL;mobile phase A (acetonitrile-methanol) and mobile phase B (water). For ELSD detection, the operating parameters were as follows:N2 low rate of 1.6 L·min-1;drift tube temperature of 42.6 ℃;gain value of 4. Result: TLC method could be omployed to distinguish Rhizoma Polygonati Odorati from its fake effectively. There was a good linear relationship for PODI in the range of 0.514 5-9.261 0 μg (r=0.999 7), and the average recovery (n=6) was 103.09% with the RSD of 1.52%. Conclusion: The method was simple, accurate and reliable, and it might provide the basis for the more effective quality control of Polygonati Odorati Rhizoma.关键词：Polygonati Odorati Rhizoma;thin layer chromatography;HPLC-ELSD;quality control
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to optimize the extraction technology of protein in Gekko Swinhoanis. Method: The proper extraction method was selected by comparing the effect of ultrasonic and reflux extraction on protein extraction rate of Gekko Swinhoanis. Then single factor analysis was done for four influencing factors, such as pH, ration of liquid to material, temperature and time. The optimized extraction technology was investigated using orthogonal test with the content of protein as the index for the first time. Result: The protein content by ultrasonic extraction was significantly higher than that by reflux extraction. The best extraction condition of protein in Gekko Swinhoanis was proposed, that's the powder of Gekko Swinhoanis was extracted for 80 minutes with 20 times amount of water (pH 12) by ultrasonic. Conclusion: The optimum extraction condition was stable, reliable and feasible, and could provide a experimental basis for formulating the quality standards and further study of bioactive substances of traditional Chinese medicine Gekko Swinhoanis.关键词：Gekko Swinhoanis;protein;content determination;single factor exploration;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: The study was performed to establish the RP-HPLC gradient elution method for determination of three glycosides and gastrodine contents in Gastrodiae Rhizoma. Method: RP-HPLC gradient elution method was used. The extracts were separated on a ZORBAX-SB-C18 analytical column(4.60 mm×250 mm,5 μm) with CH3OH-0.1% phosphoric acid as mobile phase, the UV detection wavelength was set at 270 nm, the flow rate fixed at 0.6 mL·min-1and the column temperature was maintained at 25 ℃. Result: The linear ranges of gastrodine,uridine,guanosine and adenosine were 0.286-4.29 μg(r=0.999 9), 0.028 9-0.433 5 μg(r=0.999 7), 0.066 8-1.002 μg(r=1.000 0), 0.135 6-2.034 μg(r=1.000 0), respectively. Conclusion: This method is fast, simple and accurate, which can be used for quality control of Gastrodiae Rhizoma.关键词：gastrodine;uridine;guanosine;adenosin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The study was aimed to determine the contents of paeniflorin,ferulic acid and hyperoside in Tiaojing Huoxue pills by HPLC. Method: The chromatographic column was Elipse-C18(4.6 mm×150 mm,5 μm) with acetonitrile-0.1% phosphoric acid (14:86) as mobile phase.The flow rate was fixed at 1.0 mL·min-1,the column temperature was maintained 25 ℃ and the detector was DAD.The detection wavelengths were set at 230 nm,316 nm for paeniflorin, ferulic acid and hyperoside, respectively,the detection wavelength of hyperoside was set at 360nm. Result: There is good linear relationship within the range of 160.0-1 280.0 μg for paeniflorin, 67.5-540.0 μg for ferulic acid, and 17.2-137.6 μg for hyperoside. The average revcoveries of paeniflorin, ferulic acid and hyperoside were 98.38%(RSD of 1.51%),98.33%(RSD of 1.21%) and 98.71%(RSD of 1.27%) respectively. Conclusion: This method is simple,accurate and reproducible.It can be used for the quality control of Tiaojing Huoxue pills.关键词：Tiaojing Huoxue pills;paeniflorin;ferulic acid;hyperoside;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The study was performed to establish a HPLC method for simultaneous determination of paeoniflorin,liquiritin and cinnamic acid in Yangyin Qingfei granules. Method: Paeoniflorin,liquiritin and cinnamic acid were determined simultaneously by HPLC on a ZORBAX SB-C18 column with the mobile phase composed of acetonitrile and 0.2% phosphoric acid in gradient elution. Detection wavelength were set at 276 nm and 230 nm.The column temperature was maintained at 28 ℃. The flow rate was fixed at 1.0 mL·min-1. Result: The calibration curves of paeoniflorin, liquiritin and cinnamic acid showed good linearity within 0.2368-4.736 mg·L-1 (r=0.999 9),0.170 8-3.416 mg·L-1 (r=0.999 9), 0.035 2-0.70 4 mg·L-1 (r=0.999 9), respectively. The average recoveries of those were 99.74%, 98.82% and 98.40%, respectively. RSDs were 0.78%, 0.97% and 1.04%, respectively. Conclusion: The developed HPLC method is fast, simple and accurate and can be used for quality control of Granules of Yangyin Qingfei.关键词：Yangyin Qingfei granules;paeoniflorin;liquiritin;cinnamic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The major activity ingredients of Berberis plants are alkaloids. To compare the total alkaloids and individual alkaloid (berberine) contents in roots and stems from five origins of Berberis plants (Berberis soulieana Schneid., B. henryana Schneid., B. triacanthophora Fedde, B. gagnepainii Schneid. and B. bergmanniae Schneid.), whose roots are widely used as a traditional Chinese medicine called "Sankezhen", and provide some references for resource and quality evaluation of the medicine. Method: Acid dye colorimetry and high performance liquid chromatography (HPLC) were used to measure contents of the total alkaloids and berberine, respectively. Result: Contents for the total alkaloids in root and stem samples were in the range of 1.60% to 4.72% and 0.76% to 2.70%, while those of the berberine were 0.70% to 2.92% and 0.23% to 1.07%. With higher contents of the total alkaloids and berberine, the roots of B. soulieana Schneid., B. gagnepainii Schneid. and B. bergmanniae Schneid. are good sources of Sankezhen, meanwhile the contents were also high in stems of the three plants. Conclusion: The methods established for determination of total alkaloids and berberine were simple, rapid and reliable. Furthermore, the present study provided valuable research methods for the quality evaluation of the plants.
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发布时间：2024-01-04 - 摘要：Objective: The aim of this invostigation was to study chemical compositions of essential oil from Zidianling mixture. Method: The essential oil was extracted by hydrodistillation and separated by capillary GC. Result: Eighty-one compounds were obtained and thirty-six compounds were identified by means of indexing the spectrum storeroom, which accounted for 89.07% of total peak area. Among of them, monoterpenes and triterpenes were the main chemical compositions. The characteristic compositions of essential oil were cedrol (16.77%), caryophyllene oxide (9.57%), globulol (9.51%), trans-longipinocarveol (8.21%), 3-[(E)-3-methyl-1-butenyl]-1-cyclohexene (4.40%). Conclusion: The research provided experimental data of Zidianling mixture.关键词：Zidianling mixture;essential oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establishing the quality control standard and provide scientific basis for the utilization and development of Hedyotidis diffusa. Method: Twentytwo batches of H. diffusa were collected. The morphology, inspection, identification and the content of target components in these crude drugs were studied. TLC method was used to identify this crude drug. The contents of deacetyl asperulosidic acid methyl ester in these samples were determined by HPLC. Result: Macroscopic and microscopic characteristics of H. diffusa were described. According to the measurement results, moisture in the crude drug was equal or below 6%, the content of total ash was equal or below 13 %, the content of acid-insolubal ash was equal or below 5%. The extractum by hot water was above 10%. The spot of TLC were clear and well-aparated. The linear range of deacetyl asperulosidic acid methyl ester were 0.019-2.44 μg(r=1.000 0); the average recoveries were 98.28% and RSD of them were 2.73(n=6); the content of deacetyl asperulosidic acid methyl ester were above 0.1 mg·g-1. Conclusion: The quality is suitable for H. diffusa.关键词：Hedyotidis diffusa;quality standard;deacetyl asperulosidic acid methyl ester
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发布时间：2024-01-04 - 摘要：Objective: This study was carried out to investigate the influence of different compatibility combinations of Simiao Yongan tang on the extraction efficiency of sweroside and loganin. Method: The extraction rates of sweroside and loganin were determined by HPLC, using a SunFireTM C18 analytical column (4.6 mm×150 mm, 5 μm) in prescription dismantling of Simiao Yongan tang. The mobile phase was composed of aqueous phosphoric acid (0.1%) and acetonitrile using a gradient elution. Analytes were performed at 35 ℃ with a flow rate of 1.0 mL·min-1 and a UV detection at 250 nm. Result: The sweroside and loganin in eight groups from the original prescription and dismantlementwere detected and analyzed. The regression equations for sweroside and loganin were Y=1 179 101.85X-28 583.08(r=0.999 2) and Y=1 353 095.24X-4 113.75 (r=0.999 1), respectively. The liner ranges were 0.191 2-1.338 4 μg for sweroside and 0.026 4-0.184 8 μg for loganin. The average recoveries of sweroside and loganin were 101.19% (RSD 2.83%) and 102.59% (RSD 2.30%), respectively. Conclusion: Compared with single Lonicerae Japonicae Flos, the extraction rates of sweroside and loganin were improved in other compatibilities of Simiao Yongan tang. The whole prescription was superior to the other groups, which indicated that compatibility was preponderant and reasonable.关键词：Simiao Yongan Tang;prescription dismantlement;compatibility;sweroside;loganin;extraction rate
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发布时间：2024-01-04 - 摘要：Objective: The study was performed to analyze the chemical constituents of volatile oil from domestic Folium Sennae with GC-MS technology. Method: The volatile oil was extracted from the leaves by steam distillation. The constituents of volatile oil were identified by GC-MS technology. Result: Relative content of volatile oil was determined by peak area normalization.Totally,21 compounds were identified,accounting for 66.996% of the total volatile oil content. The principal chemical constituents of the essential oils are farnesyl acetone (16.322%), phytol (13.022%), squalene (6.864%), 6,10,14-trimethyl-2-pentadecanone (6.672%), paeonol (5.995%). Conclusion: The result provided reliable experimental data and theoretical bases for the further development and utilization of domestic Folium Sennae.关键词：domestic Folium Sennae;volatile oil;GC-MS;components analysis
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发布时间：2024-01-04 - 摘要：Objective: This article aimed to to study the main pharmacodynamic substances of pediatric compound preparation Xiefei Pingchuanling by serum pharmacochemistry. Method: UPLC-LTQ-Qrbitrap technology was used to create fingerprints of both compound extract and rat serum after treatment. Then the peaks were compared and predicted to belong to which single herb and their constituents were inferre. Result: 18 transitional ingredients in Xiefei Pingchuanling were detected and identified in rat serum, 10 of which could be directly absorbed into the bloodstream by the body;the 8 remaining prototype components may be new ingredients produced through metabolism. Conclusion: UPLC fingerprint provides much medicinal information in plasma of rats treated with Xiefei Pingchuanling, therefore, it is of great value to studies on the metabolism of traditional Chinese medicine (TCM).关键词：Xiefei Pingchuanling;serum pharmacochemistry;constituents absorbed into blood
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发布时间：2024-01-04 - 摘要：Objective: The Ginseng Radix et Rhizoma was biotransformed under the optimal condition to obtain the new product medicinal fungal substance of ginseng. This study was conducted to investigate the chemical constituents of the ethyl acetate fraction of medicinal fungal substance ginseng, to clarify the substance basis of efficacy. Method: Thin-layer chromatography, column chromatography on silica gel, Sephadex LH-20 gel chromatography and semi-preparative liquid chromatography were used for isolation and purification of the compounds, and their structures were determined by comparison of their chromatographic and spectral data with those of the authentic samples and those reported in the literatures. Result: Six compounds were identified as phthalate acid bis(2-ethylhexyl)ester (1), dibutylphthalate (2), stigmasta-5,22-dien-3-beta-ol (3), a-sitosterol(4), stigmast-22-ene-3,6-dione (5), stigmasta-3,6-dione (6). Conclusion: All compounds were isolated from the medicinal fungal substance of Ginseng Radix et Rhizoma for the first time.关键词：Ginseng Radix et Rhizoma;biotransformation;ethyl acetate fraction;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: This study was carried out to study the chemical constituents from of Polyalthia oblique. Method: Compounds from 95% ethanolic extract of P. oblique were isolated by column chromatography on silica gel, Sephadex LH-20, together with recrystallization, and their structures were identified by their physical characteristics and spectral features. Result: Eight compounds were isolated from P. oblique, and identified as alkaloids isooncodine (1), pendulamine A (2), three steroids stigmasta-4-ene-3,6-dione (3), β-sitosterol (4), stigmasterol (5), and three other compounds including hydroquinone (6), vanillin (7), octadecadienoic acid (8). Conclusion: All compounds were isolated from P. oblique for the first time. Compound 3 showed inhibition activity against Escherichia coli, with minimal inhibitory concentrations(MIC) value of 5 mg·L-1. Compound 1 showed weak inhibition activities against SPC-A-1 and A-549 tumor cells, with IC50 values of 78.6, 49.3 mg·L-1.关键词：Polyalthia oblique;chemical constituents;antimicrobial activity;antitumor activity
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发布时间：2024-01-04 - 摘要：Objective: To investigate gastrointestinal absorption of total flavonoids from Apocyni Veneti Folium in rats. Method: Taking rats in situ perfusion model,absorptivity of hyperin and isoquercitrin in gastrointestinal was counted through detecting contents of index components before and after perfused total flavonoids from Apocyni Veneti Folium by HPLC,mobile phase was acetonitrile-0.05% phosphoric acid (18.5:81.5) at a flow rate of 0.90 mL·min-1 and detection wavelength at 360 nm. Result: A verage absorptivity in stomach of hyperin and isoquercitrin were 36.92% and 36.49%,while in intetines were 47.7% and 48.25%,respectively. Conclusion: This study indicated that hyperin and isoquercitrin had good absorptivity in gastronintestinal department,but absorption of the two ingredients in intestines was better than these in stomach,it could provide scientific basis for dosage form design and clinical rational drug of total flavonoids from Apocyni Veneti Folium.关键词：gastrointestinal absorption;hyperin;isoquercitrin;total flavonoids from Apocyni Veneti Folium;in situ perfusion model
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发布时间：2024-01-04 - 摘要：Objective: To explore tissues distribution characteristics of icariin and its metabolites including icarisid Ⅰ,icarisid Ⅱ and icaritin in the kidney of osteoporosis model rats after intragastric administration of icariin. Method: Kidney tissues were gotten from rats in 40 min,1.5,2.5,4,12, 24 h after intragastric administration of icariin with dosage of 118 mg·kg-1.After pretreated biological samples,HPLC was employed to determine contents of icariin and its metabolites with mobile phase of 0.4% aqueous acetic acid(A)-methanol(B) for gradient elution(0-11 min,43%A;12-30 min,37%A;31-46 min,43%A) and detection wavelength at 270 nm. Result: At 40 min after intragastric administration,icariin(0.017 5 mg·L-1),icarisid Ⅰ(0.014 2 mg·L-1) and icarisid Ⅱ(0.012 7 mg·L-1) could be checked out;these ingredients reached high concentrations at 2.5 h and eliminated basically after 24 h.Icaritin was checked out at 2.5 h and arrived the high concentration at 4 h,but it still exited at 24 h. Conclusion: Icariin distributed fast and coexisted with its metabolites in the kidney of rats,these ingredients had higher concentrations and maintained a certain period of time in the kidney,which pointed their target may be the kidney,it matched the Chinese medicine theory that Epimedii Folium went through the liver and kidney.This established method was accurate and fast,which could provide a reference for in vivo pharmacokinetic research of icariin.关键词：icariin;icarisid Ⅰ;icarisid Ⅱ;icaritin;osteoporosis model rats
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects of monomer salvianolic acid B(Sal B) and hydroxysafflor yellow A(HSYA) in Danhong injection on neuronal with oxygen-glucose deprivation (OGD) injury, and the interaction of anti-freedom injury to optimize the combination ratio. Method: A (whether OGD), B (Sal B different doses) and C (HSYA different doses) were considered three factors, using completely randomized 2×3×3 factorial design, cell morphology was observed under inverted phase contrast microscope, and xanthine oxidase method was used to detect intracellular superoxide dismutase(SOD) activity. Result: Factor A, B and C had a significant effect on SOD activity (P<0.01), besides, factor B and C, factor A and B had an interaction (P<0.01), and when Sal B 75 μmol·L-1, HSYA 60 μmol·L-1, SOD activity was highest. Conclusion: Sal B combined with HSYA plays a protection role and against hypoxic-ischemic reperfusion injury of hippocampal neurons in vitro through. anti-free radical damage关键词：Chinese herbal monomer compound;salvianolic acid B;hydroxysafflor yellow A;cerebral ischemia reperfusion injury;hippocampal neurons;factorial design
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects of Ginkgo terpene lactones meglumine injection (GTLMI)on cerebral focal ischemia reperfusion injury induced by middle cerebral artery occlusion (MCAO) in rats. Method: Ninety six male SD rats were divided into six groups, such as sham, model, nimodipine(0.4 mg·kg-1),and GTLMI(4.8,2.4,1.2 g·kg-1)groups. All of rats were given corresponding drugs by tail vein injection before modeling. Except the sham group, the cerebral ischemic stroke model was used. The effects of GTLMI on the brain neurological deficit score, and the brain infraction areas percentage were observed.The influence of GTLMI on the activities of superoxide dismutase (SOD), content of malondialdehyde (MDA),creatine kinaes BB isozyme (CK-BB) in blood serum and the activities of Caspase-3 in brain were assayed. Result: Compared with MCAO group, GTLMI could obviously reduce the infract area, increase the activities of SOD, and decrease the content of MDA, CK-BB in blood serum and Caspase-3 in brain. Conclusion: GTLMI can protect the focal cerebral ischemia reperfusion injury in rats.The mechanisms may be related to recovering the blood brain barrier, decreasing the content of CK-BB, scavenging free radicals and reduction of the expression of Caspase-3.关键词：Ginkgo terpene lactones meglumine injection;cerebral ischemia reperfusion injury;creatine kinase-BB;superoxide dismutase;malondialdehyde;Caspase-3
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发布时间：2024-01-04 - 摘要：Objective: To invesigate the effect of Jiangzhi Kangyanghua mixture on expression of 5-lipoxygenase(5-LO) pathway in apolipoprotein E-deficient mice (ApoE-/-mice). Method: Male ApoE-/- mice(n=40;8 weeks old) were obtained and were given a high-fat diet. These mice were divided into four groups as follows:model group,low dose of Jiangzhi Kangyanghua mixture group(17.41 g·kg-1·d-1),high dose of Jiangzhi Kangyanghua mixture group(69.66 g·kg-1·d-1) and positive drug group (simvastatin, 0.05 g·kg-1·d-1). The ten normal C57BL/6J mice with same genetic background were as normal control group,fed with common diet. All mice were administered orally for a month. Then, all mice were killed, the serum and abdominal aorta were saved. HE stating of abdominal aorta was established, also 5-LO mRNA expression level was detected by RT-PCR. The serum protein of leukotrienes B4 (LTB4) and leukotrienes D4(LTD4) were detected by ELISA. Result: The gene and protein expressions of 5-LO in the aortic plaques(P<0.01),as well as the serum levels of LTB4 and LTD4(P<0.01) in atherosclerosis group were markedly increased compared with those of the normal control group. Compared with the model group, the expression of Jiangzhi Kangyanghua mixture of high dose could significantly down regulate 5-LO gene (P<0.01), pathological section HE staining showed that the high dose Jiangzhi Kangyanghua mixture changed obviously vascular tissue compared with the model group, which was similar to the positive drug group, showing the mixture had a good therapeutic effect on atherosclerosis;ELISA results showed the high dose Jiangzhi Kangyanghua mixture could significantly reduce the blood LTB4 and LTD4 levels, compared with the model group (P<0.01). Conclusion: These findings demonstrate that Jiangzhi Kangyanghua mixture can reduce the 5-LO level, improve the morphology of atherosclerotic vascular, reduce lipid deposition, but also can reduce the serum LTB4 and LTD4 levels.关键词：Jiangzhi Kangyanghua mixture;5-lipoxygenase;cysteinyl;leukotrienes;inflammation plaque
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发布时间：2024-01-04 - 摘要：Objective: To study the depressive behavior induced by simulated weightlessness in rats and the possible mechanism. Method: Simulating weightlessness(SW) 14 days to rats, the open field test, the novel object test, the novelty suppressed feeding test, the forced swimming test and the shuttle box test were applied to evaluate the depressive-behavior in rats. The concentration of serum corticosterone and adrenocorticotropic were measured by enzyme-linked immunosorbent assay (ELISA), and the changes of the brain-derived neurotrophic factor was measured by western blotting experiment. Result: The results of the open field test were as follows:compared with the control group, the ability of locomotor activity and the number of climbing wall decreased in the SW model rats. The latency of novel object test and the novelty suppressed feeding test in the rats of the model group were longer than those of the control group. The immobility time in the forced swimming test was longer and the number of active avoidance of shuttle box test were lower than the control group. Moreover, the hormone levels of serum corticesterone(CORT) and adrenocorticotropic hormone(ACTH) increased in the rats of the model group than those of the control group. While, the expression of brain-derived neurotrophic factor brain-derived neurotrophic factor(BDNF) in the brain of rats in the model group declined than that of the control group. Conclusion: To simulate weightlessness for 14 days can induce the depressive behavior in rats.关键词：simulate weightlessness;depressive behavior;corticesterone;adrenocorticotropic hormone;brain-derived neurotrophic factor
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Xiaofu Tongjie fang on functional constipation defecation function and gastrointestinal motility index. Method: Fifty adult male SD rats were randomly divided into blank control group, model group, Xiaofu Tongjie fang high dose group,Xiaofu Tongjie fang medium dose group, Xiaofu Tongjie fang low dose group with ten rats in each group. SD rats with functional constipation model were estabilished by oral administration of compound diphenoxylate tablets for 4 weeks. After the success of modeling, the treatment group was given Xiaofu Tongjie fang for 3 weeks. Performance of different groups of rats, food intake, feces weightand general behavior. Rats' colonic electromyography was detected by Power Lab. The serum contents of motilin and gastrin were detected by ELISA. Result: Compared with the control group, in the model group the body weight, food intake, feces quantity were decreased, with significant difference (P<0.01). Compared with the model group, the body weight, food intake and fecal quantity of rats were increased in Xiaofu Tongjie fang high and medium dose group, which were markly increased in the high dose group(P<0.01). Compared with the blank group, the frequency of in vivo colonic electromyography slow wave was decreasedand the amplitudewas increased, in all groups of Xiaofu Tongjie fang, which were markly changed in the high dose group (P<0.01). The serum contents of motilin and gastrin in all groups of Xiaofu Tongjie fang group were decreased as compared with the blank group, and which were increased markly for high dose group as compared with the control group(P<0.01). Conclusion: Xiaofu Tongjie fang can treat functional contipation by impoving the symptoms and the gastrointestinal motility in functional constipation rats.关键词：Xiaofu Tongjie fang;functional constipation rats;gastrointestinal motility
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect and mechanism of Longli Pingchuan paste to episodes of pediatric asthma induced airway inflammation. Method: Forty-eight rats were randomly divided into blank group,model group,dexamethasone group(1 mg·kg-1) and Longli Pingchuan paste high,middle and low dose group (74.8,37.4,18.7 g·kg-1),8 rats each group. Ovalbumin(OVA) was used to copy the rat model of asthma, administration lasted 1 week. The allergic symptoms rhinitis mucosa and lung tissue, pathologic morphological changes between the nasal mucosa and lung tissue of asthmatic rats were observed, and lung tissue inflammation cell apoptosis rate, the expression of proto oncogene Fas mRNA and B cell lymphoma/leukemia-2(Bcl-2) mRNA were detected. Result: In addition to the blank group, other groups showed allergic rhinitis symptoms allergic asthma, and eosnophil infiltration and similar pathologic morplological changer were seen in the nasal mucosa and lung tissue in model group;compared with blank group, the numbers of asthma rats in the model group increased significantly(P<0.01);compared with model group, Longli Pingchuan paste significantly reduced the number of asthma rats(P<0.01);compared with blank group, inflammation cell apoptosis rate was significantly lower in the model group(P<0.01);inflammation cell apoptosis rate was significantly higher than the model group(P<0.05),positive expression rate of control gene Fas mRNA of proinflammatory cell apoptosis increased significantly(P<0.01),positive expression rate of control gene Bcl-2 mRNA of inhibiting inflammation cell apoptosis decreased significantly(P<0.01) in lung tissue in Longli Pingchuan paste high and middle dose group. Conclusion: Longli Pingchuan paste can obviously inhibit airway inflammation in asthma rat, the mechanism may be related to promoting inflammation cell apoptosis and reversing Fas/Bvl-2 ratio imbalance.关键词：Longli Pingchuan paste;infantile asthma;airway inflammation
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发布时间：2024-01-04 - 摘要：Objective: To analyze the rationality of Eryan Huguo decoction(EYHGD). Method: Ammonia water stronger nebulization was used to analyze the effects of preventing cough, and mouse's auricle swelling induced by xylene were used to analyze the effects of antiinflammatory. The indexes of pharmacodynamics were used to screen the suitable extraction solvent. Its dismantle compatibility was studied by removing one kind of herbal medicine of the complex prescription. Result: The suitable solvent was 55% ethyl alcohol. Each kind of herbal medicine in the prescription had some effects, the sequence of which was as follows:Lysionotus pauciflorus >Bergenia scopulosa >Bulbophyllum odoratissimum >Polygonum cuspidatum. Conclusion: The prescription formula of EYHGD is reasonable. Its compatibility relation is as follows:L. pauciflorus is principal drug, B. scopulosa is ministerial drug, B. odoratissimum is adjunctive drug and P. cuspidatum is messenger drug, which was similar to the primitive prescription.关键词：Eryan Huguo decoction;preventing cough;anti-inflammatory;prescription research
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of sodium tanshinone ⅡA sulfonate (STS) on human umbilical vein endothelial cell(HUVEC) inflammatory injury by visfatin. Method: The human umbilical vein endothelial cells (HUVECs) were cultured in vitro, after the cells were treated with visfatin(250 μg·L-1)for 4 hours, STS was added to the cells in different concentration of(30,60,120 mg·L-1) for 24 hours. The levels of inflammatory cytokines as high sensitivity C reactive protein(hs-CRP), tumor necrosis factor α(TNF-α)and metal matrix protein (MMP)-9 were determined by enzyme-linked immunosorbent assay(ELISA). The activation of mitogen-activated protein kinases(MAPKs) was assayed by Western blotting. In order to evaluate the role of MAPKs in the inflammatory injury by visfatin, HUVECs were pretreated with MAPKs inhibitor, and visfatin was added after the pretreatment, then the levels of hs-CRP,TNF-α and MMP-9 was measured. Result: Visfatin led to an increase level of hs-CRP, TNF-α and MMP-9,while the increase of above mentioned cytokines induced by visfatin could be inhibited by STS in a dose dependent way. STS could also inhibit the activation of p38 MAPK and extracellular signalregulated kinase (ERK), but no significant inhibition of Jun N-terminal kinase(JNK). The p38 MAPK inhibitor SB203580(25 μmol·L-1),ERK inhibitor PD98059 (25 μmol·L-1) and JNK inhibitor SP600125(25 μmol·L-1) markedly decreased visfatin-induced enhance in hs-CRP, TNF-α and MMP-9 expression. Conclusion: Inflammatory response of HUVEC could be induced by visfatin, the mechanism may be related to high expression of inflammatory cytokines, which may be mediated by MAPK phosphorylation signaling pathway. STS attenuates visfatin-induced inflammatory injury by interfering with the modulation of MAPK signal pathway.关键词：human umbilical vein endothelial cell;visfatin;sodium tanshinone ⅡA sulfonate (STS);mitogen-activated protein kinases;inflammatory cytokines
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发布时间：2024-01-04 - 摘要：Objective: To investigate the neuroprotective effect of salvianolic acid B(Sal B) against acute focal cerebral ischemia in mice. Method: The acute cerebral ischemic model induced by medial cerebral artery occlusion(MCAO) was reproduced.The modeling mice were given Sal B 11.25, 22.5, 45 mg·kg-1 or Nimhorizon 30 μg·kg-1 by tail vein injection after MCAO immediately. The model group and control group were treated with the same volume of physiological saline. After 6 h, the following indexes were observed including:the behavior, brain index, brain water content, cerebral infarction volume and brain tissue pathological morphology were observed. The glutamate contents in brain tissue was determined by colorimetric methods with commercial kit. Result: After MCAO for 6 h, compared with model group, the nerve functional behavior score, brain index, brain water content and cerebral infarction volume of Sal B dose groups in mice were significantly reduced(P<0.05,P<0.01). According to the results of pathological tissue section, all the Sal B groups could reduce the brain neurons nucleus pycnosis, nuclear dissolve degree, irregular degree, and improve the pathological injury of brain tissue ischemia. The different Sal B dose groups could significantly reduce the content of glutamic acid (P<0.05, P<0.01). Conclusion: Sal B can amelioratee neurological damage in mice with cerebral ischemia injury, and reduce cerebral edema, decrease glutamate contents, inhibit the excitatory amino acid neurotoxicity action. Sal B has neuroprotective effects on acute focal cerebral ischemia in mice.关键词：salvianolic acid B;medial cerebral artery occlusion;glutamate
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发布时间：2024-01-04 - 摘要：Objective: To investigate the total flavone of Hawthorn Leaves(TFHL) on the therapeutic effect and mechanism of spatial learning and memory impairment of the vascular dementia rat. Method: Sixty-four SD male rats were randomly divided into sham control group, model group, TFHL 70, 140 mg·kg-1 group, Sixteen in each group. Permanent bilateral carotid artery ligation was used to prepare vascular dementia model.TFHL 70, 140 mg·kg-1 group were given for consecutive 36 days. Morris water maze was used for evaluation of learning and memory abilities of rats. Colorimetric detection was used to detect hippocampus acetyl cholinesterase (AChE), choline acetyltransferase(ChAT) content. Result: Compared with the sham control group, in vascular dementia model group learning and memory capacity significantly reduced, escape incubation period extended significantly (P<0.05), the number of crossing the platform area significantly reduced (P<0.05), significantly reduced percentage of target quadrant distance(P<0.05);ChAT activity was significantly decreased (P<0.05), AChE activity was significantly increased (P<0.05). TFHL and Yinxingye pian could improve learning and memory ability, shorten the the rats escape latency (P<0.05),increase the number of across the platform and the platform quadrant percentage of swimming distance (P<0.05), enhance ChAT activity (P<0.05), reduce AChE activity (P<0.05). Conclusion: TFHL can improve learning and memory impairment of vascular dementia rat, may enhance ChAT activity, reduce the activity of AChE, increase the acetylcholine content of brain tissue, restore cholinergic nerve system function related to chronic cerebral ischemia in rats.关键词：total flavone of hawthorn leaves;vascular dementia;choline acetyltransferase;acetylcholinesterase
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发布时间：2024-01-04 - 摘要：Objective: To observe hypoglycemic and hypolipidemic effects of Xiaoke capsules on type 2 diabetic rats. Method: Except eight Wistar rats were as the normal control,the rest sixty-two type 2 diabetic rats were induced by feeding with high-fat and high-suger diet and intraperitoneal injection of low-dose streptozotocin (STZ,35 mg·kg-1). According to the values of blood glucose concentration (>7.0 mmol·L-1), the rats were randomly divided into diabetic model group, three Xiaoke capsules groups of high dose (0.4 g·kg-1), medium dose (0.2 g·kg-1) and low dose (0.1 g·kg-1), and positive control (glibenclamide,0.25 mg·kg-1) group. During 36 d of continuous administration, body weights of the rats were measured weekly. The values of fasting blood glucose concentration were measured on 0,8,16,24, 32 d of the experiment. After the last administration, 12 h latter, the rats were decapitated after anesthesia. Blood of the rats were collected for the analysis of serum insulin(INS), total cholesterol(TC), triglycerides(TG) and low-density lipoprotein(LDL-C) levels. Pancreas of the rats were collected for histopathological examination. Result: Compared with normal group, model group, blood glucose was significantly increased (P<0.01), serum INS was significantly decreased (P<0.01), TC,TG,LDL-C was significantly increased(P<0.01). Compared with model group, Xiaoke capsules showed significant hypoglycemic effect (P<0.05),and significant difference was observed compared to diabetic model group on serum INS, TC, TG and LDL-C levels (P<0.05). Histopathological results indicated that more numbers and much more regular shape of islets and islet cells in the medium dose and high dose of treatment groups were observed with comparison to the model group, and the morphology of endocrine cells in islets were close to those of the normal group. Conclusion: Xiaoke capsules can decrease blood glucose, regulate blood lipids in type 2 diabetic rats.关键词：Xiaoke capsules;type 2 diabetics;blood glucose;blood lipid
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发布时间：2024-01-04 - 摘要：Objective: To observe the analgesic effect of Xiongzhi Shigao tang and the influence on the level of nitric oxide(NO),nitric oxide synthase(NOS), calcitonin gene-related peptide(CGRP)in blood plasma and 5-hydroxytryptamine(5-HT),5-hydroxyindole acetic acid(5-HIAA)in the brain tissue in the rat models of migraine. Method: The mice and rats were divided into blank control group,model group,three doses groups of Xiongzhi Shigao tang (mice:6,12,24 g·kg-1; rat:3,6,12 g·kg-1·d-1),Chinese medicine and western medicine control groups.The chemical and physical pain threshold were observed. Nitroglycerin was used to make rat migraine model. The changes of behavior were detected. The contents of plasma NO,NOS,CGRP were measured by the way of radioactive immunoreaction or spectmphotometry. Contents of 5-HT and 5-HIAA in brain tissue were assayed HPLC. Result: Compared with the normal group, the first phase of paw licking time and the second phase of paw licking time were increased significantly in the model group of formalin test(P<0.01). In the test of migraine model induced by nitroglycerin, the forelimb scratching frequency of rats were increased markedly compared with the blank control group (P<0.01) during the period of 0 min to 30 min. The contents of NO,NOS,CGRP in model group were higher than blank group significantly(P<0.05),but the content of 5-HT and 5-HIAA in the brain was decreased (P<0.05).Xiongzhi Shigao tang could suppress Faure Marin induced pain reaction effectively, which could significantly reduce rats' first phase of paw licking time and second phase of paw licking time compared with the model group (P<0.05,P<0.01). And Xiongzhi Shigao tang could significantly increase the pain threshold of mice induced by hot plate compared with the blank control group (P<0.05,P<0.01).Compared to model group, the contents of NO,NOS and CGRP in high and middle dosage group of Xiongzhi Shigao tang were decreased (P<0.05, P<0.01), and the content of 5-HT were increased in brain (P<0.05). Conclusion: The remedial Xiongzhi Shigao tang can treat migraine through adjusting the contents of NO,NOS,CGRP and 5-HT.This might be one of the possible mechanisms of Xiongzhi Shigao tang in the treatment of migraine.关键词：Xiongzhi Shigao tang;migraine;nitric oxide;nitric oxide synthase;calcitonin gene-relatiod peptide;5-hydroxytryptamine;5-hydroxyindole acetic acid
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发布时间：2024-01-04 - 摘要：Objective: Discuss curative effect of addition and subtraction of Paiqi decoction taken orally combined with external application of Chinese medicine at navel to the recovery of gastrointestinal function and influence to levels of gastrin(GAS),motilin(MTL) and vasoaetive intestinal peptide(VIP) after abdominal surgery. Method: One hundred and twenty-six patients with gastrointestinal dysfunction after abdominal surgery were randomly divided into western medicine group (62 cases) and Chinese medicine group (64 cases) by random number table.After the operation,both groups' patients received corresponding basic treatment.Patients in western medicine group received mosapride citrate tablets,5 mg/time,3 times/day,and taken orally.Patients in Chinese medicine group received addition and subtraction of Paiqi decoction combined with external application of Chinese medicine at navel.Course of treatment in two groups were both 7 days.After the operation,recovery time of gurgling sound,the first aerofluxus and defecation time were recorded. At the first,second,third,fifth and seventh day after the operation,scores of patients' main symptoms and signs were recorded.and at the first,second and third day after the operation,levels of GAS,MTL and VIP in blood were detected by radioimmunoassay. Result: After the operation,recovery time of gurgling sound,the first aerofluxus and defecation time in Chinese medicine group were all shorter than those in western medicine group(P<0.01).At the second day after operation,compared with the first day,scores of patients' main symptoms and signs in western medicine group increased(P<0.01),and then decreased gradually.At the second to seventh day after operation,there was a decreasing trending of scores of patients' main symptoms and signs in Chinese medicine group(P<0.01),and at the second,third,fifth and seventh day after the operation,those scores were lower than in western medicine group(P<0.01).At the second day after the operation,compared with the first day,levels of GAS and MTL in western medicine group were decreased,and level of VIP increased(P<0.01).At the third day after the operation,compared with the second day,levels of GAS and MTL in western medicine group were increased,and level of VIP decreased(P<0.01).At the second and third day after the operation, there was a increasing trending of levels of GAS and MTL in Chinese medicine group(P<0.01),and at the second day after the operation,level of VIP increased,but there was no statistical significance from the differences.At the third day after the operation,level of VIP in Chinese medicine group decreased significantly(P<0.01).At the second and third day after the operation,levels of GAS and MTL in Chinese medicine group were higher than those in western medicine group,and level of VIP was lower than western medicine group(P<0.01).The excellent and good rate of gastrointestinal function in Chinese medicine group was 62.5% superior to 40.32% in western medicine group(P<0.05). Conclusion: Paiqi decoction combined with external application of Chinese medicine at navel can promote gastrointestinal function to recovery quickly,ameliorate symptoms and signs, its' mechanism of action may be related to improving levels of GAS and MTL and inhibiting level of VIP.关键词：abdominal operation;gastrointestinal dysfunction;Paiqi decoction;gastrin;motilin;vasoactive intestinal polypeptide
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发布时间：2024-01-04 - 摘要：Objective: The observe clinical effects of treating cor pulmonale at acute phase(phlegm and blood stasis type indicates the lungs)through traditional Chinese medicine combined with western medicine and influence of hemorheology,endothelin-1(ET-1), thromboxane B2(TXB2)and 6-Ketone prostaglandin F_la(6-Keto-PGF_la)'s level. Method: Ninety patients were randomly divided into control group (45 cases) and observation group (45 cases).Patients in control group received conventional western medicine therapy,including controlling oxygen cure,anti-infection,expectorant,smooth wheezing,heart failure and other comprehensive measures.Based on the treatment of control group,patients in observation group added and subtract treatment of Xiaoxianxiong decoction,Sanzi Yangqin decoction and Xuefu Zhuyu decoction.Treatment courses continued for 4 weeks.Measure arterial carbon dioxide partial pressure(PaCO2),oxygen partial pressure(PaO2),pulmonary artery pressure(mPAP)before and after treatment. Test hemorheology, ET-1,TXB2 and 6-Keto-PGF_la's level before and after treatment.Record treatment of main symptoms and signs scores every week. Result: The total effective rate of curative efficacy in observation group was 91.11% superior to 75.56% in control group(P<0.05).The level of mPAP and PaCO2 in observation group was lower than that in control group,while the level of PaCO2 in observation group was higher than that in control group after treatment(P<0.01).Observation group's improvement of blood rheology indexes whole blood viscosity,plasma viscosity, fibrinogen, platelet aggregation rate and erythrocyte sedimentation rate were better than those data in control group(P<0.01).The level of ET-1 and TXB2 in observation group were lower than the data in control group.At the same time, observation group's 6-Keto-PGF_la level was higher than that in control group after treatment(P<0.01).At the first,second,third and fourth mouth after the treatment,main symptoms and signs scores in observation group were lower than those in control group(P<0.01). Conclusion: Traditional Chinese medicine combined with western medicine can relieve symptoms and signs,ameliorate ventilatory function of lung,hypercoagulability of blood,high viscosity of blood and congestion of pulmonary circulation,reduce pulmonary artery pressure in treating cor pulmonale at acute phase,and it can improve clinical efficacy.关键词：chronic pulmonary heart disease;traditional chinese combined western medicine treatment;endothelin-1;thromboxane B2;6-Ketone prostaglandin F_la
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发布时间：2024-01-04 - 摘要：Objective: Discuss curative effect of conservative treatment of using Qiangyao Xuanbi decoction through taken orally and hot compress to cure degenerative lumbar spinal stenosis(DLSS) and influence to hemorheology and serous inflammatory factor. Method: Ninety-five patients with DLSS were randomly divided into control group (48 cases) and Chinese medicine group (47 cases).In control group received glucosomine and indomethacin erterucoated tablets,1-2 tablets/time,2 times/day.Patients in control group received chinese herbs orally and ironing with Chinese medicine,course of treatment in two groups were both 4 weeks.Patients'stepping abilities in two groups were observed.Visual analogue scales(VAS) of lumbar and leg ache in two groups were graded.The overall symptoms were graded by Japan orthopedic assoctation(JOA) scales.Before and after treatment,hemorheology and serous inflammatory factor were detected,which including interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor(TNF-α)and C-reactive protein(CRP). Result: The excellent rate in Chinese medicine group was 76.6% superior to 56.25% control group(P<0.05).After treatment,compared with the time before treatment,scores of stepping abilities and VAS of lumbar and leg ache in two groups decreased(P<0.01),and JOA scores increased(P<0.01).In follow-up period,scores of stepping abilities and VAS of lumbar and leg ache in two groups increased(P<0.01),and JOA scores decreased at the same time(P<0.01).After treatment and in follow-up period, stepping abilities and VAS of lumbar and leg ache in Chinese medicine group were lower than those in control group(P<0.01),and score of JOA was higher than in control group(P<0.01).Amelioration of whole blood viscosity(low shear and high shear),plasma viscosity,fibrinogen and hematokrit of hemodynamic indexes in Chinese medicine group were superior to those in control group(P<0.01).Levels of CRP,IL-6,IL-1βand TNF-α in Chinese medicine group were lower than those in control group(P<0.01). Conclusion: Conservative treatment of Qiangyao Xuanbi decoction curing degenerative lumbar spinal stenosis can remit clinical symptoms and improve the abilities of patients' lives,its' mechanism of action may be related to ameliorating blood circulation,restraining inflammatory substances and relieving inflammatory injury.关键词：degenerative lumbar spinal stenosis;Qiangyao Xuanbi decoction;ironing;hemorheology;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: Discussed synergism and attenuation function of adjuvant of Shengxue Qingdu decoction in treating acute leukemia. Method: Sixty patients with acute leukemia were randomly divided into control group (30 cases) and observation group (30 cases) according to the order in hospital.Patients with acute non-lymphocytic leukemia in the control group received daunorubicin and cytosine arabinoside as DA scheme.patients with lymphocytic leukemia received vincristine,daunorubicin,L-asparaginase and prednisone by VALP method.Each group received corresponding auxiliary symptomatic treatment.Based on the treatment of control group,patients in the observation group added dialectical treatment of addition and subtraction of Shengxue Qingdu decoction,1 dose/day,taken along with chemotherapy and continued taking for 4 weeks after the ending of chemotherapy.Before and after the ending of chemotherapy,patients' hepar and renal function,blood coagulation and peripheral hemogram were monitored.At the forth week after the ending of chemotherapy,condition of returning to normal of inner and peripheral hemogram three-line were monitored.Before chemotherapy and at the forth week after chemotherapy,level of T lymphocyte subgroups of peripheral blood and natural killer cell(NK) were detected by flow cytometer. Result: The total effective rate in the observation group was 83.33% superior to 56.67% in the control group(P<0.05).After chemotherapy,compared with the time before chemotherapy,liver function glutamic-pyruvic transaminase and glutamic oxalacetic transaminase and renal function(blood urea nitrogen and serum creatinine decreased(P<0.01),but which in observation group were lower than those in control group(P<0.01).Hemoglobin(HB) of peripheral blood and platelet(PLT) increased(P<0.01),the degree of increasing in observation group was more significant than in control group(P<0.01).Index of blood coagulation such as prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT) and fibrinogen(FIB) were decreased,PT,APTT and FIB in observation group were lower than those in control group(P<0.01).At the forth week after chemotherapy,cases of returning to normal of Hb,PLT and NK in observation group were more than those in control group(P<0.05).Levels of CD3+,CD4+,CD4+/CD8+ and NK in observation group were higher than those in control group(P<0.01). Conclusion: Shengxue Qingdu decoction can reduce patients' damage of hepatic function of chemotherapy in treating acute leukemia and the inhibition of chemotherapy to medullary hematopoiesis,and it can adjust patients' blood coagulation,ameliorate symptom of bleeding,adjust cellular immune function and improve anti-tumor ability of bodies,and there is a good synergism and attenuation function.关键词：acute leukemia;Shengxue Qingdu decoction;synergism and attenuation;cellular immune
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发布时间：2024-01-04 - 摘要：Objective: Discuss influence of modified Eryin decoction jiawei to glucose fluctuation and impact on oxidative stress and function of vascular endothelium in treating type two diabetes mellitus(T2 DM). Method: Eighty-six patients with T2 DM were randomly divided into control group (43 cases) and observation group (43 cases) by random number table.Patients in control group received repaglinide tablets,1-2 mg/time,3 times/day,and took it at the fifteenth minute before meal.and patients got subcutaneous injection of insulin glargine injection before sleeping.Based on the treatment of control group,patients in observation group added modified Eryin decoction jiawei,1 dose/day.Course of treatment in two groups were both 12 weeks.Before and after treatment,continuous glucoses which including standard deviation of blood glucose level(SDBG),mean amplitude of glycemic excursions(MAGE),mean of daily differences(MODD), and mean postprandial glucose excursion (MPPGE) were monitored;and fasting blood-glucose(FBG),fasting insulin(FINS),two hours' postprandial blood glucose(2h PBG) and glycosylated hemoglobin(HbAlc) were detected,and indexes of homeostasis model assessment of insulin resistance(HOMA-IR),secretion function of pancreas islet B cell and insulin sensitivity(ISI) were calculated.and Levels of methane dicarboxylic aldehyde(MDA),superoxide dismutase(SOD),adiponectin(APN),von willebrand factor(vWF) and nitric oxide(NO) were detected. Result: After treatment,indexes of FBG,2 hPG,HbA1c and HOMA-IR in observation group were lower than those in control group,but indexes of HOMA-IS and ISI were higher than those in control group(P<0.01).and SDBG,MAGE,MODD and MPPGE in observation group were lower than those in control group(P<0.01).levels of APN and NO in observation group were higher than those in control group(P<0.01),but level of vWF was lower than in control group(P<0.01). And level of SOD in observation group was higher than in control group(P<0.01),but level of MDA was lower than in control group(P<0.01). Conclusion: Eryin decoction jiawei can reduce blood glucose of patients with T2 DM,Regulate intraday glucose fluctuation,keep stability of blood glucose,ameliorate secretion function of pancreas islet B cell and improve bodies' insulin sensitivity.And it can also relieve oxidative stress and damage of vascular endothelium, delay the onset of diabetes and its complications.关键词：type 2 diabetes mellitus;glucose fluctuation;Eryin decoction jiawei;insulin resistance;insulin sensitivity;oxidative stress;function of vascular endothelium
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发布时间：2024-01-04 - 摘要：Objective: This study aimed to explore the relationships of procalcitonin(PCT),high sensitivity C-reactive protein (hs-CRP) and central venous catheter-related bloodstream infections (CRBSI) in the intensive care unit(ICU) by measuring serum PCT and hs-CRP levels,to provide methods of treatment and clinic experience and reduce unnecessary removal of central venous catheter. Method: In 62 cases of suspected CRBSI patients,blood samples were analyzed by enzyme-linked fluorescent assay to determine PCT concentration,and by solid-phase double-antibody sandwich immunoassay test for the detection of hs-CRP concentrations;CRBSI diagnosis relied on the blood sample culture and tip culture methods,then the samples were divided into CRBSI group and non-CRBSI group. Receiver operating curve (ROC) was plotted,and the area under the receiver operating characteristic curve (AUC) was compared. Result: 26 patients were diagnosed as CRBSI,with significantly higher (P<0.01) serum PCT level (11.44±6.92) μg·L-1 and hs-CRP level (124.60±56.30) mg·L-1 than the PCT levels (1.93±3.08) μg·L-1 and hs-CRP levels (62.48±49.38) mg·L-1 in the non-CRBSI group.The area under the curve (AUC) for PCT was 0.929,whereas a cut-off value of 2.5 μg·L-1 provided the best sensitivity and specificity with a value of 92.3% and 80.6%,respectively. In contrast, the AUC for hs-CRP was 0.802,whereas a cut-off value of 86.62 mg·L-1 provided the best sensitivity and specificity with a value of 76.9% and 77.8%,respectively.The area under the ROC curve, sensitivity,specificity,positive predictive value,and negative predictive value in PCT diagnose for CRBSI were higher than those of CRP. Conclusion: The serum PCT and hs-CRP levels could be used as sensitive indicators to monitor the CRBSI patients of ICU.PCT has higher sensitivity and specificity than CRP,hence monitoring the PCT level dynamicly is more conducive to early clinical prevention and treatment.关键词：catheter-related bloodstream infections;central venous catheter;procalcitonin;high sensitivity C-reactive
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this research was to study the clinical efficacy of Fuxin decoction in the treatment of heart failure, and provide the reference basis for the future clinical treatment. Method: One hundred and twenty patients with heart failure cured in our hospital from Feb 2012 to Dec 2013 were randomized into the control group (treated with the routine anti-heart failure therapy such as strong heart and dieresis) and the observation group (Fuxin decoction added based on the control group), with 60 patients per group. The efficacy of heart function, traditional Chinese medicine(TCM) syndrome scores, adverse reactions, left ventricular end diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF) and E/A value on pre-and post-treatment in the two groups were observed and compared. Result: compared with the control group, the heart function efficacy (96.7% vs 86.7%) and the TCM syndrome scores efficacy (91.7% vs 73.3%)were significantly increased, the incidence of adverse reactions was obviously reduced (1.7% vs 13.3%)in the observation group, P<0.05;compared with the control group, the LVEF(47.0±1.0 vs 44.4±1.2)and E/A values (1.3±0.4 vs 1.0±0.3)were significantly increased in the post-treatment in the observation group, P<0.05. Conclusion: The efficacy of Fuxin decoction in the treatment of heart failure is significant, it can improve the patient's heart function and prognosis quality with less adverse reactions and higher treatment safety, which is worthy of clinical promotion.关键词：Fuxin decoction;heart failure;efficacy
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发布时间：2024-01-04 - 摘要：Objective: To study clinical efficacy of Herba Hedyotidis Diffusae as major prescription combining the radiotherapy in treating esophageal cancer for the guidance of the better treatment. Method: Eighty patients with esophageal in our hospital during the period from Jan 2010 to Jan 2013 were randomized into the control group (radiotherapy) and the observation group (Herba Hedyotidis Diffusae combined with radiotherapy). The clinical efficacy, life quality and the occurrence of adverse reaction of patients in two groups after treatment, and the tumor necrosis factor-β(TNF-β), high sensitive C creactive protein (hs-CRP), serum tumor markers such as CA125 and CEA in the two groups were observed. Result: Compared with the control group, the total effective rate was significantly increased (60.0% vs 42.5%) and the life quality was obviously impoved (80.0% vs 62.5%) in the observation group after treatment, P<0.05.The comparison of adverse reactions during the treatment period between the two groups was not statistical difference. Compared with the control group, the levels of TNF-β and hs-CRP were significantly reduced (P<0.05), the comparison of CA125 and CEA levles were not statistical difference. Conclusion: The prescription mainly consisting of Herba Hedyotidis Diffusae as major prescription combining with the chemotherapy in treating esophageal cancer delivers significant effect, and it can effectively improve the life quality of patients, extend two-year survival rate of patient, and less adverse reactions are found, being worthy of clinical promotion.关键词：Herba Hedyotidis Diffusae;radiotherapy;esophageal cancer
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to evaluate the efficacy and safety of cinobufacini injection on chronic hepatitis B. Method: We searched the cochrane central register of controlled trials (CCTR), PubMed, CBM, CNKI, VIP databases up to Dec. 2012.The methodological quality assessment and data extraction of the including studies were conducted by two reviewers independently according to the inclusion and exclusion criteria. Meta-analyses were performed for homogeneous studies using RevMan 4.2.10 software. Result: A total of 17 studies involving 1 635 patients met the inclusion criteria. None of the studies enforced allocation concealment and performed blinding. Conclusion: Cinobufacini injection might be effective in normalizing alanine aminotr ansferase(ALT) levels, clearing hepatitis B virus(HBV) DNA, achieving virus seroconversion and improving hepatic fibrosis, without any serious adverse effects. However, the overall combination effect analysis could not be conducted, because the including studies were performed in small sample with low-quality. More evidence is needed to support this analysis.关键词：cinobufacini injection;chronic hepatitis B;efficacy;safety;systematic review
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发布时间：2024-01-04 - 摘要：Objective: To analyze the composing principles of prescriptions for bone metastases with traditional Chinese medicine(TCM) inheritance system. Method: The prescriptions used for bone metastases from China National Knowledge Infrastructure(CNKI) database were collected, the composing principles were analyzed using hierarchical clustering method in the TCM inheritance system after data entry. Result: Based on the analysis on 43 cases of prescriptions were mined from the database which come up with 29 core medicine pairs and eight new formulas. Seven of the eight new formulas contained tonic herbs. Psoraleae Fructus-Epimedii Folium-Cinnamomi Ramulus-Cyperi Rhizoma,Psoraleae Fructus-Drynariae Rhizoma-Cinnamomi Ramulusi-Cyperi Rhizoma,Psoraleae Fructus-Drynariae Rhizoma-Eucommiae Cortex-Poria were widely used Psoraleae Fructus,Drynariae Rhizoma, Epimedii Folium,which belonged to kidney channel, were in high frequency and showed a high-level association as a whole. Conclusion: TCM inheritance system provides an important method in exploring the composing principles for bone metastases through obtaining frequency of dominant empirical analysis as well as association of implicit empirical analysis. The basic therapeutic principle strengthening the body resistance and using kidney channel herbs provides available exploration and is beneficial for clinical use.关键词：bone metastases;channel tropism;regularity;composition principle TCM inheritance system
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发布时间：2024-01-04 - 摘要：With the deeply understanding of the disease pathogenesis, particularly the complex ones, multi-target drug design has become a new tendency of drug development. Nowadays, scientists pay more and more attention on traditional Chinese medicine (TCM), due to its overall regulating characteristics of multi-component and multi-targets. Systematic pharmacological evaluation of the active compounds in Chinese herbal formula and the identification of the corresponding drug targets are the critical issues in the modern research of Chinese materia medica and the TCM. However, the current TCM drug development patterns and strategies have no longer meet the demand of TCM development. Literature researchers found that with the rapid development of systems biology, all kinds of systems biology technology is widely used in Chinese medicine research and development. At the same time, CADD technology shows unique advantages in active ingredients selection, target detection, toxicity prediction, and prescription mechanism exposition in traditional Chinese medicine. The wide application of systems biology and CADD technology, will provide new idea to clarify the mechanism of action of TCM. Hence, this manuscript aims to analyze the current TCM development patterns, review the application of systems biology technology, and CADD technology in the field of traditional Chinese medicine research and development. Base on the review and analysis, a new TCM development strategy that combining systems biology and CADD technology was proposed. This proposed strategy will provide a reference approach to study the complicated theoretical system and achieve modernization of TCM.关键词：systems biology;computer aided drug design;traditional Chinese medicine;research trends;research and development pattern
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发布时间：2024-01-04 - 摘要：Objective: This article was in order to analyse determination of ginseng saponins in Chinese patent drugs,then analysed its detection interference.Five kinds of different dosage-forms of Chinese patent drugs including ginseng saponins were selected,each kind of dosage selected two varieties to carry on comparison,HPLC determination of ginseng saponins in different forms was compared,it showed that ingredients contents had a different effect on determining the contents of ginseng saponins.According to different circumstances,the most suitable chromatographic system should choose by combining ingredients-efficacy elaluation,this article can provide a reference for quality control of Chinese patent drugs including ginseng saponins.关键词：ginseng saponins;Ginseng Radix et Rhizoma;forms;mobile phase;chromatography system
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发布时间：2024-01-04 - 摘要：The research progress on hyaluronic acid (HA) in recent years was summarized. Consulting 30 literatures both domestic and abroad, the preparation methods,derivative structures and application of HA were reviewed. Hyaluronic acid is considered as a new pharmaceutical excipient,with stable physical and chemical properties as well as good compatibility and moisturizing effect,also it can be simply biodegraded. At present,as for the preparation, research and application of HA in China, there is still a large gap compared with the advanced research abroad, however, HA was the vast market potential which provides huge space for development and application.关键词：hyaluronic acid;derivatives;biological materials
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发布时间：2024-01-04 - 摘要：This article was too review the research progress in the production of gallic acid using microbial transformation method. Literatures on microbial transformation in gallic acid production were summarized, including material, mechanism, fermentation condition and production mode. Microbial transformation method has incomparable advantages over traditional alkali and acid catalysis methods in production of gallic acid. Research focusing on the breeding of strains with high conversion rate and the optimization of culture conditions laid the foundation for the further research in the improvement of the yield of gallic acid. Microbial transformation method was a good way to the development and application potential in gallic acid production.关键词：tannic acid;gallic acid;microbial transformation
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