最新刊期
卷 20 ， 期 16 ， 2014
- 摘要：Objective: Traditional Chinese medicine (TCM) safety has drawn more concerns from domestic and abroad because of its adverse reactions that were occasionally reported.In this article, recent achievements and research progress in TCM safety evaluation were reviewed, which included more research grants on TCM toxicology, GLP practice, TCM injections safety evaluation, systematic study of ‘toxic TCMs’, and so on.Finally, TCM safety problems that currently exist, such as classification of ‘toxic TCMs’, methodology on the control of TCM toxicity, early discovery of its toxicity, research and development of a new drug from ‘toxic TCMs’, and so on, were discussed, and then, a study strategy of TCM toxicology was proposed to be aimed at overcoming the problems.关键词：toxicity of traditional Chinese medicine(TCM);toxicology;toxic TCM;Rhei Radix et Rhizoma;Bupleuri Radix;Senecionis Scandentis Hebra
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发布时间：2024-01-04 - 摘要：Objective: To synthesize and characterize superporous hydrogel hybrids of konjac glucomannan (KGM-SPHH). Method: KGM-SPHH was prepared by foam polymerization method.Synthesis conditions, including the amount of each reacting agents, were optimized by single factor tests according to swelling kinetics of these obtained samples.Morphology and chemical structure of KGM-SPHH were analyzed by SEM and FTIR. Result: Optimized synthesis conditions were as follows:50% acrylamide (AM) 2 mL, 50% acrylic acid (AA) 0.3 mL, 2.5% N, N', -methylene-bis-acrylamide 2 mL, 10% pluronic f127 0.3 mL, 20% ammonium persulfate 0.2 mL, konjac glucomannan (KGM) 0.1 g, 20% N, N, N', N', -tetramethylethylene diamine 0.2 mL, NaHCO3 0.2 g.A homogeneous KGM-SPHH could be prepared under proper ratio of reagents, and it could quickly absorb large amount of water to reach swelling equilibrium.KGM-SPHH showed a large number of connected pores under SEM, AA-co-AM and cross-linked KGM in FTIR spectra. Conclusion: Homogeneous KGM-SPHH with porous structure and quick swelling property could be obtained by foam polymerization method.关键词：konjac glucomannan;superporous hydrogel hybrids;foam polymerization method;swelling ratio;acrylic acid;acrylamide
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发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasonic wave-enzymatic assisted semi-biotic extraction technology for Zanthoxyli Radix. Method: With yields of total alkaloids, nitidine chloride and dry extract as indexes, orthogonal test was adopted to optimize extraction technology by taking ultrasonic power, extraction time and solvent amount as factors, then extraction time was selected by dynamic process.HPLC was employed to determine the content of nitidine chloride with mobile phase of acetonitrile-water-phosphoric acid-triethylamine (30:70:0.15:0.15) and detection wavelength at 271 nm;UV was adopted to determine the content of total alkaloids at 328 nm. Result: Optimal extraction process of Zanthoxyli Radix was as follows:after pretreated by cellulase-pectinase, extracted thrice by 5 times the amount of sodium hydrogen phosphate-citric acid buffer with pH of 2.0, 7.5 and 8.3, extraction time of 18, 15, 12 min, respectively;ultrasonic power of 250 W;yields of total alkaloids, nitidine chloride and dry extract were 1.20%, 0.135% and 14.65% with RSD of 1.75%, 1.86% and 1.23%, respectively. Conclusion: Dry extract yield of this optimized process is significantly higher than ethanol reflux process, while extracting rates of nitidine chloride and total alkaloids are equal with or little lower than the later, this study could provide a reference for industrial production of Zanthoxyli Radix.关键词：ultrasonic wave-enzymatic;semi-biotic extraction technology;Zanthoxyli Radix;dynamic process;nitidine chloride;total alkaloids
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发布时间：2024-01-04 - 摘要：Objective: To prepare emodin-loaded soluplus polymeric micelles (Emo-PMs) and evaluate their quality. Method: Emo-PMs were prepared by film dispersion method using an amphiphilic copolymer soluplus.Emo-PMs were characterized by particle size analyzer, transmission electron microscopy (TEM), X-ray diffraction (XRD);release curve was determined by dynamic membrane dialysis;HPLC was employed to determine entrapment efficiency and drug loading with mobile phase of methanol-0.1% phosphoric acid(75:25) and detection wavelength ar 437 nm. Result: Emo-PMs showed spherical with a mean particle size of (65±3.8)nm, polydispersity index of 0.099±0.022, Zeta potential of-(12.7±0.19) mV, average encapsulation efficiency of (88.25±3.51)% and average drug loading of (4.51±0.72)%.Emodin entrapped in polymer micelles with amorphous or molecular form;release methanism of Emo-PMs fitted Higuchi equation. Conclusion: These prepared Emo-PMs had exhibited in vitro sustained release, their particle size, encapsulation efficiency and drug loading were controllable.关键词：emodin;soluplus;Polymeric micelles;quality evaluation;cumulative release;drug loading;encapsulation efficiency
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发布时间：2024-01-04 - 摘要：Objective: To optimize matrix formulation of Fumikang gels. Method: With viscosity (32℃), thixotropy (32℃) and stability as dependent variables, amounts of sodium carboxymethyl cellulosef (CMC-Na), glycerine and polysorbate 80 (tween-80) as independent variables, single factor test was adopted to screen ranges of each independent variable, matrix formulation of Fumikang gels was optimized by central composite design-response surface methodology. Result: Optimal formulaiton was as following:CMC-Na 3.5 g, glycerine 8.0 g, tween-80 5.0 g;these measured values of stability, thixotropy and viscosity were 19.5 point, 7 686.9 Pa· s-1· mL-1 and 4 325.9 mPa· s;these predicted values were 20 point, 7 815.843 Pa· s-1· mL-1 and 4 442.731 mPa· s;absolute values of deviations between the measured value and the predicted value were 2.50%, 1.65% and 2.63%, respectively. Conclusion: These prepared Fumikang gels had appropriate viscosity, good stability and thixotropy, this study could provide a reference for clinical practice of this preparation.关键词：central composite design-response surface methodology;Fumikang gels;integrated balance method;rheology;Cnidii Fructus;Sophorae Flavescentis Radix;Calamina
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发布时间：2024-01-04 - 摘要：Objective: To optimize water extracting technology of Jiawei Baiteng granules. Method: Withcomposite score of contents of berberine hydrochloride, paeoniflorin and solid-containing as index, orthogonal test was adopted to optimize extraction process by taking the amount of water, extraction time and boiling times as factors.Contents of berberine hydrochloride and paeoniflorin were determined by HPLC coupled with DAD detector, mobile phase consisted of 10 mmol· L-1 potassium dihydrogen phosphate solution (A)-acetonitrile(B) with gradient elution program of(0-9 min, 19.5% B;9-10 min, 19.5-45% B;10-25 min, 45% B), detection wavelengths were set at 347 nm and 231 nm, respectively. Result: Optimal extracting technology was as follows:decocted 3 times with 10 times the amount of water for 1 h at each time;under these conditions, contents of berberine hydrochloride, paeoniflorin and solid-containing were 0.092 5 g, 0.333 7 g and 23.69%, respectively. Conclusion: This optimizeprocesswas reasonable, feasible and reproducible, which could guide industrial production of Jiawei Baiteng granules.关键词：Jiawei Baiteng granules;Thlaspi arvense;berberine hydrochloride;paeoniflorin;comprehensive evaluation method;solid-containing content;Phellodendri Chinensis Cortex
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of anti-diabetic active parts from Rosae Laevigatae Fructus. Method: With composite score of in vitro anti-α-amylase and anti-oxidation activities as dependent variable, ethanol concentration, solid-liquid ratio, extraction time and times as independent variables, test data were analyzed by Design Expert 8.05 software, then multivariate quadratic regression equation was established, Box-Behnken response surface methodology was adopted to optimize extraction process of anti-diabetic active parts from Rosae Laevigatae Fructus. Result: Optimal process parameters were as follows:extracted twice with 11 times the amount of 85% ethanol for 120 min per time;average inhibition rate of α-amylase was 95.39% with RSD of 4.59%, average clearance rate of DPPH was 91.47% with RSD of 4.12%, composite score was 0.967 2, which was fit with its predictive value of 0.977 8. Conclusion: Optimization of extraction process was scientific and conformed with clinical practical application by biological activity evaluation.关键词：Rosae Laevigatae Fructus;Box-Behnken experimental design;response surface methodology;composite score;α-amylase;DPPH radical scavenging
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total tannins in Polygoni Cuspidati Rhizoma et Radix and investigate its hygroscopicity. Method: The content of total tannins was determined by complexometric titration. Taking extracting amount of total tannins as index, extraction temperature was selected by single factor test, orthogonal test was adopted to optimize extraction process with acetone concentration, extracting times and solid-liquid ratio as factors.Total tannins powder was placed in environment with constant temperature, humidity and pressure, weight change was measured periodically to investigate its hygroscopic properties. Result: Optimum extraction process was as following:extracted thrice with five times the amount of 30% acetone for 30 min each time;average extracting amount of total tannins was 102.8 mg· g-1 with RSD of 1.25%.Critical relative humidity of total tannins in Polygoni Cuspidati Rhizoma et Radix was about 50%, it reached hygroscopic balance after 24 h in the environment with constant temperature, humidity and pressure. Conclusion: Optimized extraction process was stable and feasible, total tannins in Polygoni Cuspidati Rhizoma et Radix had strong hygroscopicity.关键词：Polygoni Cuspidati Rhizoma et Radix;total tannins;hygroscopicity;critical relative humidity;complexometric titration
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of different proportions between Puerariae Lobatae Radix and Ephedrae Herba on dissolution of puerarin. Method: HPLC was used to determine the content of puerarin with mobile phase of methanol-water (25:75) and detection wavelength at 250 nm, methodological study of puerarin from couplet medicines of Puerariae Lobatae Radix and Ephedrae Herba was established.Effects of couplet medicines of Puerariae Lobatae Radix and Ephedrae Herba with different proportions (3:1, 2:1, 4:3, 1:1, 1:2, 1:3) on dissolution of puerarin were investigated. Result: This established determination was stable and feasbile with average recovery of 99.67% (RSD 2.2%).The maximum dissolution (2.408 g· L-1) of puerarin was achieved when proportions of Puerariae Lobatae Radix and Ephedrae Herba was 4:3, it was closed to Puerariae Lobatae Radix decoction (2.455 g· L-1), the minimum dissolution (2.254 g· L-1) of puerarin was achieved when proportions of Puerariae Lobatae Radix and Ephedrae Herba was 2:1. Conclusion: Different proportions of Puerariae Lobatae Radix and Ephedrae Herba had some influence on dissolution of puerarin.关键词：couplet medicines of Puerariae Lobatae Radix-Ephedrae Herba;compatibility proportion;puerarin;dissolution
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of total tannins from Juglans mandshurica leaf by macroporous resin. Method: Adsorption and elution capacities of AB-8, D101, NKA-9, Daion HP-20 macroporous resin were investigated by static adsorption-elution tests, single factor tests were adopted to optimize purification process of total tannins from J. mandshurica leaf with sample volume, water amount, elution solvent as factors.The content of total tannins was measured by casein, detection wavelength was set at 760 nm. Result: AB-8 macroporous resin was selected, its optimal purification technology was as follows:the concentration of sample solution 2.672 g· L-1, sample volume of 250 mL, elution rate of 2 BV· h-1, washed impurity with 6 BV of water, collected 10 BV of 50% ethanol eluent;elution rate and purity of total tannins were 73.32% and 35.02%, respectively. Conclusion: AB-8 macroporous resin had goog separation performance for total tannin from J. mandshurica leaf, this optimized purification process was suitable for industrial production.关键词：Juglans mandshurica leaf;Macroporous resin;total tannins;casein law
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Herba Polygoni Chinensis and other Thirteen kinds of medicinal materials in Ganyou capsules. Method: With composite score of extraction amounts of polydatin and resveratrol as index, orthogonal test was adopted to optimize water extraction technology of Ganyou capsules by taking the amount of water, soaking time, extraction time and times as factors.Contents of polydatin and resveratrol were determined by HPLC, mobile phase consisted of methanol-0.5% phosphoric acid solution (35:65), detection wavelength was set at 306 nm and flow rate was 0.9 mL· min-1. Result: Optimum extraction conditions were as follows:soaked 0.5 h with eight times the amount of water, extracted twice for 2 h per time;under these conditions, average extracting amounts of polydatin and resveratrol were 0.676, 0.198 mg· g-1, RSD were 0.56% and 0.91%, respectively. Conclusion: This optimized extmction technology was stable and feasible, it would provide experimental basis for improvement of quality stability and clinical drug safety of Ganyou capsules.关键词：Ganyou capsules;extraction technology;polydatin;resveratrol;Polygoni Cuspidati Rhizoma et Radix;Abri Herba
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of immunologically active componentsinAstragali Radix. Method: Taking extracting amounts of astragaloside and total polysaccharides from Astragali Radix, dry extract yieldas comprehensive evaluation index, methods of 1% NaOH soaking, 1% CaO boiling and water extraction were investigated by single factor test.HPLC was adopted to determine the content of astragaloside with mobile phase of acetonitrile-water (30:70), air pump pressure of 0.4 MPa, ELSD drift tube temperature at 105℃ and carrier gas flow rate of 2.7 L· min-1;the content of total polysaccharides from Astragali Radix was determined by UV, detection wavelength was set at 489 nm. Result: By extracting of these three kinds of methods, extracting amounts ofastragaloside were 0.826, 1.016, 0.842 mg· g-1, extracting amounts oftotal polysaccharides were 14.49, 5.82, 17.04 mg· g-1, respectively;but dry extract yield had no significant difference. Conclusion: Water extraction process could achieve better extraction effect by comparing with the other two methods, itcould reduce production costs for industrial mass production of immunologically active componentsinAstragali Radix.关键词：Astragali Radix;astragaloside;total polysaccharides;extraction method;dry extract yield
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total saponins from Chaenomelis Fructus. Method: Taking yield of total saponins as index, extraction method was selected by single factor test, orthogonal test was adopted to optimize extraction process with ethanol concentration, extracting temperature, ratio of material to liquid and extraction time as factors.UV was employed to determine the content of total saponins with detection wavelength at 550 nm. Result: The best extraction process was as following:extracted 3 h with 20 times the amount of 80% ethanol at 90℃;yield of total saponins from Chaenomelis Fructus was 1.69%. Conclusion: This process was stable and feasible, it could provide a reference for development of series products of Chaenomelis Fructus.关键词：Chaenomelis Fructus;total saponins;single factor test;orthogonal test;extraction process
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发布时间：2024-01-04 - 摘要：Objective: To compare effects of different drying methods on contents of active components in Cortex Daphnes. Method: HPLC was adopted to determine contents of syringin, daphnetin and umbelliferone with mobile phrase of acetonitrile-0.1% formic acid (15:85) and detection wavelength at 265, 326 nm, respectively.The content of total coumarins was determined by UV at a wavelength of 326 nm.Effects of different drying methods on contents of active components in Cortex Daphnes were investigated by single factor test. Result: The content line of daphnetin by different drying methods was vacuum drying>oven drying at 80℃>oven drying at 40℃>oven drying at 50℃>drying in the shade>oven drying at 60℃>oven drying at 105℃>drying in the sun, the content line of umbelliferone was oven drying at 80℃>drying in the shade>vacuum drying>oven drying at 105℃>oven drying at 50℃>oven drying at 60℃>oven drying at 40℃>drying in the sun, the content line of syringin was oven drying at 40℃>drying in the shade>drying in the sun>vacuum drying≈oven drying at 50℃>oven drying at 60℃>oven drying at 80℃>oven drying at 105℃, the content line of total coumarins was vacuum drying>drying in the shade>oven drying at 50℃>drying in the sun>oven drying at 60℃>oven drying at 80℃>oven drying at 40℃>oven drying at 105℃. Conclusion: Vacuum drying could maximally preserve active components, followed by natural drying in the shade and oven drying at 50℃.It recommended natural drying in the shade when processing in production place;if conditions allowed in origin or pieces manufacturers, vacuum drying at 35℃ was the best method to dry Cortex Daphnes, followed by oven drying at 50℃.关键词：Cortex Daphnes;drying method;daphnetin;umbelliferone;total coumarins;syringin
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发布时间：2024-01-04 - 摘要：Objective: This study investigated the changes of chemical ingredient between the crude and processed Psoralea Fructus to provide scientific evidence for elucidating the reason that the toxicity of extremely dry Psoralea Fructus was alleviated after salt-processed. Method: Ultra-performance liquid chromatography method (UPLC) coupled with quadrupole time of flight mass spectrometry (Q-TOF/MS) was used for the analysis of the crude and the processed Psoralea Fructus. And the results were analyzed by Markerlynx software Result: There were significant differences between the crude and processed Psoralea Fructus. The contents of psoralen, isopsoralen, bavachin, corylin, isobavachalcone, bavachalcone showed an obvious increase, while the contents of bavachromanol and bakuchiol decreased in salt-processed Psoralea Fructus. This could be the material foundation for the alternation of pharmacological effects after processing. Conclusion: By Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (OPLS-DA), the indicative marker, like bavachinin, bavachalcone and bavachin, could be used to identify the crude and processed Psoralea Fructus. The experimental results can not only be of great importance to the processing principle of salt-processed Psoralea Fructus, but also provide crucial basis for clarifying effective material basic of salt-processed Psoralea Fructus.关键词：Psoralea Fructus;salt-processed Psoralea Fructus;UPLC-Q-TOF/MS;flavone
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发布时间：2024-01-04 - 摘要：Objective: This study aimed to establish the RAPD molecular marker system and to probe into the genetic background of Pueraria lobata. Method: RAPD analyses of selected plants from eleven various habitats in China were carried out using arbitrary primers, in combination with the chemical investigation of puerarin. Result: The percentage of puerarin ranged from 3.26% to 7.10%. A total of 45 DNA fragments were obtained using 8 primers, 37 of which were polymorphic, accounting for 82.22%, which can tell that the samples had a high level of genetic diversity. Conclusion: P. lobata has significant genetic differentiation that further support the prospects for germplasm selection.关键词：Pueraria lobata;puerarin;genetic diversity;random amplified polymorphic DNA
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发布时间：2024-01-04 - 摘要：Objective: This study was to investigate the dynamic changes of five phenylethanols from Ligustri Lucidi Fructus affected by different steaming time spans with wine. Method: The contents of echinacoside, acteoside, salidroside, tyrosol and hydroxytyrosol in Ligustri Lucidi Fructus were determined by HPLC in crude Ligustri Lucidi Fructus and wine-steamed Ligustri Lucidi Fructus after different processing time(4, 8, 12, 16, 20 and 24 h). Result: Within 24 h, as wine-steamed time prolonged, the content of echinacoside decreased, while Salidroside increased thoughtout the steaming process, tyrosol and hydroxytyrosol increased before they reach the stable state, however acteoside increased at first and then decreased. Obviously, salidroside showed the greatest change of the five phenylethanols. Conclusion: The contents of phenylethanols from Ligustri Lucidi Fructus changed significantly after processing. In Ligustri Lucidi Fructus, echinacosideand acteoside were in qualitative alteration and salidroside, tyrosol and hydroxytyrosol were in quantitative alteration after alcohol-steaming. This could provide experimental evidence to the processing mechanism, as well as to the processing standardization and research of the slices of Ligustri Lucidi Fructus.
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to determine the content of chlorovaltrate and valjatrate B in total Iridoids of Valerianae Jatamansi Rhizoma et Radix. Method: HPLC method was adopted. The chromatographic column was waters C18 column (4.6 mm×250 mm, 5 μm); the mobile phase for chlorovaltrate and valjatrate B was acetonitrile water (60:40), (50:40); the detection wavelength for chlorovaltrate and valjatrate B were 254, 203 nm; the flow rate was 1.0 mL· min-1; the column temperature was 30℃. Result: The fine linear correlation displayed when the injection volumn were 15.5-155 pg (r=0.998 6), 20-200 pg (r=0.998 7) for chlorovaltrate and valjatrate B in total iridoids of Valerianae Jatamansi Rhizoma et Radix, respectively. The contents of chlorovaltrate and valjatrate B were 8.91 mg· g-1 and 7.00 mg· g-1, respectively. Conclusion: The contents of chlorovaltrate and valjatrate B in total Iridoid of Valerianae Jatamansi Rhizoma et Radix were determined to provide evidence for quality control of total iridoids of Valerianae Jatamansi Rhizoma et Radix.关键词：total iridoids of Valerianae Jatamansi Rhizoma et Radix;chlorovaltrate;valjatrate B
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发布时间：2024-01-04 - 摘要：Objective: This study was aimed to investigate the chemical constituent of Kadsura longipeduculata collected from Guizhou province, and to explore the active ingredients in this herb. Method: Methods including silica gel column chromatography, macroporous resin column chromatography, molecular sieve chromatography, recrystallization and HPLC preparation were used. And the constituents were separated and purified from the petroleum ether and ethyl acetate fractions of ethanol extract of K. longipeduculata. According to their physical and chemical properties, coupled with the data of 1H-NMR, 13C-NMR and EIS-MS, the structures were elucidated. Result: Eight compounds were isolated and identified as tubiferolide methyl ester(1), schizandronic acid(2), sorghumol(3), schizandriside(4), lancilactone C(5), kadsurarin(6), β-sitosterol(7), (+)-anwulignan(8). Conclusion: Compounds 2-5 are isolated from the plant for the first time. 1, 2, 3, 5 are triterpene compounds, and 4, 6, 8 are lignans compounds.关键词：Kadsura longipedunculata;chemical constituents;lignans;triterpene
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发布时间：2024-01-04 - 摘要：Objective: This study aimed at establishing the quantitative model of moisture content in Gynoste Pentaphylli Herba Seu Radix by near infrared reflectance spectroscopy(NIRS). Method: The near infrared spectra of Gynoste Pentaphylli Herba Seu Radix with different batch numbers from various areas were collected, and were subject to the analysis by TQ Analyst 8.0 software. Near infrared spectra of all the samples were pretreated with First Derivative and Norris filter (3 segment length and 3 gap between segments). The wavebands containing 10 000-4 000 cm-1 were collected and the first 10 factors were used. The calibration model was built with partial least squares (PLS). Result: The quantitative calibration model had good correlation coefficients(R) and low root mean square error of cross validation(RMSECV), with a r value equal to 0.968 8, RMSECV value equal to 0.303 and root mean square error of prediction(RMSEP) value equal to 0.284. The average rate of recovery of validation was 101.14% (n=11), RSD 2.16%.At the same time, NIR instrument was good in precision.The samples had great performance in reproducibility and stability within 2 h. Conclusion: The quantitative calibration model of moisture content in Gynoste Pentaphylli Herba Seu Radix was proven to be credible and could be used to predict the moisture content in Gynoste Pentaphylli Herba Seu Radix accurately. The simple, fast and non-destructive advantages of NIRS can be used for quality control and exploitation of Gynoste Pentaphylli Herba Seu Radix.关键词：Gynoste Pentaphylli Herba Seu Radix;moisture content;near infrared spectroscopy
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to develop a RP-HPLC method for simultaneous determination of six ingredients (chlorogenic acid, caffeic acid, galuteolin, forsythoside A, forsythin and quercetin) in Lonicerae Japonicae Flos and Forsythiae Fructus extracts. Method: The chromatographic separation was achieved on a Thermo Scientific ODS-2 HYPERSIL (4.6 mm×200 mm, 5 μm) column with gradient elution of acetonitrile and 0.2% (v/v) formic acid aqueous solution at a flow rate of 1.0 mL· min-1. The detection wavelength was set at 280 nm, and the column temperature was set at 30℃. Result: The ranges for linear correlation of chlorogenic acid, caffeic acid, forsythoside A, galuteolin forsythin and quercetin were 40.40-404.0 mg· L-1 (r=0.999 5), 0.2032-2.032 mg· L-1 (r=0.999 9), 30.20-302.0 mg· L-1 (r=0.999 8), 1.012-10.12 mg· L-1 (r=0.999 9), 6.980-69.80 mg· L-1 (r=0.999 9), and 1.980-19.80 mg· L-1 (r=0.999 9), respectively. The recoveries of the injection samples were 102.6%, 99.6%, 100.4%, 98.3%, 100.8% and 99.7% with RSD of 0.9%, 2.0%, 1.1%, 0.4%, 2.1% and 0.9%, respectively. Conclusion: The method is simple and accurate. It can be applied for the quality control of the Lonicerae Japonicae Flos and Forsythiae Fructus extracts.
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发布时间：2024-01-04 - 摘要：Objective: This study was to develop a method for determination of trace elements in Erythronium sibiricum's bulb. Method: An inductively coupled plasma mass spectrometry(ICP-MS) method was used for the determination of the contents of 12 trace elements in E. sibiricum's bulb after microwave digestion of the samples. Result: Satisfactory linearity of working curves for the 12 elements was obtained, with all the correlation coefficients over 0.999 3.The precision of measurement ranged from 1.45% to 3.58%.The recoveries of detection was in the range of 90.1% to 115.6%. The results showed that E. sibiricum's bulb contained rich trace elements, such as Fe, Al, Zn, Mn and Cu. Conclusion: The proposed method had the advantages of simplicity, speediness and sensitivity. It can satisfy the need for determination of trace elements.关键词：Erythronium sibiricum;trace elements;ICP-MS
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发布时间：2024-01-04 - 摘要：Objective: The study aimed to establish a method to determine the content of protocatechuic acid in Euonymus Fortunei. Method: High performance liquid chromatography (HPLC) was used for the determination of protocatechuic acid in Euonymus Fortunei. This method was established on a Ulitimate XB-C18(2) column with the mobile phase consisting of methanol-0.6% glacial acetic acid (27:73) and the flow rate of 0.7 mL·min-1 at the room temperature, the detection wavelength was 260 nm. The injection volume was 10 μL. Result: The regression equation of protocatechuic acid was A=1.39×107C-1.12×104, the correlation coefficient r=0.999 9.The protocatechuic acid in the range of 0.005-1 μg displayed a good linear relationship. And the average recoveries of protocatechuic acid were 105.3%, RSD were 1.52%. Conclusion: The determination of the protocatechuic acid content in Euonymus Fortunei by this method is accurate and reliable, which can be used for quality control of the medicines.关键词：Euonymus Fortunei;protocatechuic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The study was conducted to establish an HPLC method for the determination of quercetin and naringenin in the moxa flavone cream. Method: Quercetin and naringenin were separated on Gemini C18 RP-column(4.6 mm×250 mm, 5 μm), the flow rate was 1.0 mL· min-1, column temperature was 25℃, and the detection wavelength was 330 nm, the injection volume was 10 μL, with 0.5% aqueous acetic acid as the mobile phase A was and acetonitrile as B for gradient elution. Result: Quercetin and naringenin showed good linear relationship in the range of 0.010 26 to 0.307 8 μg (r=0.999 9), 0.009 87 to 0.296 1 μg (r=1) respectively.Their average recovery rates were 97.32% with RSD of 3.3% and 97.22% with RSD of 2.7%, respectively. Conclusion: The method is simple, rapid, reproducible, and it can be used for the quality of control moxa flavone cream.
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发布时间：2024-01-04 - 摘要：Objective: This study aimed at inspecting the content changes of amino acids in curculigo. Method: The HPLC analysis was performed after derivatization by using phenyl isothiocyanate (PITC) as a derivative reagent. Ecosil C18 (4.6 mm×250 mm, 5 μm) chromatographic column was used, and the column temperature was 41℃, with a detection wavelength of 254 nm and a flow rate of 1.0 mL· min-1. Dual gradient elution method was applied in determination of amino acid content in curculigo before and after toasting with wine. Result: The concentration of 17 amino acids showing good linear relationship (r=0.996 7-0.999 6) was in the range of 7.51 to 144.96 mg· L-1.The average recovery was between 95.3% and 104.2%. The RSD was less than 3.0%. The total contents of 17 amino acids in curculigo increased by 23.39% after roasting with wine, while the total contents of 7 essential amino acids increased by 36.19%. Conclusion: Roasting with wine can increase the amino acid contents in curculigo for better nourishing function.关键词：curculigo;pre- column;amino acids;derivationphenylisothiocyanate (PITC)
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for ultrasonic-assisted extraction and high performance liquid chromatography (HPLC) determination of shikonin in the roots of Arnebiae Radix. Method: Shikonin was extracted by ultrasonic-assisted-methyl alcohol method. The HPLC determination condition consisted of Shim-pack CLC-ODS-18 (4.6 mm×250 mm, 10 μm) and shikonin was detected at 516 nm wavelength by using methyl alcohol-water(85∶ 15)as the mobile phase, column temperature at room temperature and flow rate 1.0 mL·min-1. Result: Shikonin had a good linearity relation in the range 0.127-1.143 μg (r= 0.999 8), regressive equation were Y=338 763X-198 347. The average recovery was 99.3%, RSD 1.18% (n=6). In contrast with refluxing process, the extraction efficiency of ultrasonic extraction was 1.29 times. Conclusion: The methods of ultrasonic extraction and HPLC determination were proved to be accurate, reliable and repeatable. It can be used to determine natural shikonin content of Arnebiae Radix easily.关键词：shikonin;HPLC;ultrasonic-assisted extract;Arnebiae Radix
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of gardenin, geniposide, albiflorin, paeoniflorin, liquiritigenin, paeonol in Danzhi Xiaoyaosan by HPLC. Method: The Cosmosil 5 C18 column(4.6 mm×250 mm, 5 μm)was used with a mobile phase of acetonitrile(A)- 0.5% phosphoric acid solution (B) gradient elution, the flow rate was 1 mL· min-1, and the detection wave-lengths were set at 230 nm. Result: The linear ranges were 0.009 46-0.946 mg· L-1 for gardenin, 0.009 58-0.958 mg· L-1 for geniposide, 0.008 68-0.868 mg· L-1 for albiflorin, 0.004 88-0.488 mg· L-1 for paeoniflorin, 25.0-500 mg· L-1 for liquiritigenin, 25.0-500 mg· L-1 for paeonol respectively. The average recoveries of three constituents were 96.1%, 104.3%, 100.5%, 94.7%, 97.4%, 95.4% with the RSD of 1.32%, 2.55%, 0.67%, 1.29%, 1.36%, 2.38%. The contents of six compounds, gardenin, geniposide, albiflorin, paeoniflorin, liquiritigenin, paeonol in Danzhi Xiaoyaosan were 1.203, 0.960, 0.720, 0.157, 0.104, 0.127 mg· g-1. Conclusion: Determination of Danzhi Xiaoyaosan could effectively control the product quality of Danzhi Xiaoyaosan.
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发布时间：2024-01-04 - 摘要：Objective: The study aimed to compare the difference of volatile components in lavender by different extraction methods. Method: Volatile components from lavender were extracted by steam distillation (SDE), microwave extraction (MWE), head space-solid phase microextraction (HS-SPME) respectively, and analyzed by gas chromatography-mass spectrometry (GC-MS). Result: There was a certain difference among the volatile compounds extracted by the above three methods. Totally, 54 volatile components were identified, including 17 alcohols, 11 alkenes, 11 esters, 3 ketones, 2 aldehydes, and 10 other components such as phenols, amines, caryophyllene oxide and so on. Among the detected compounds in the samples, 34 terpenoids with pharmacological activity were identified, and 7 common compounds were identified by three methods, namely α-terpinene, linalool oxide, linalool, linalyl acetate, cryptone, (Z)-β-farnesene and caryophyllene oxide. Characteristic components were specific to each extraction method:hotrienol, nerol, nerolidol, cuminol, α-cedrol and camphor were characteristic components from SDE method; α-santalene, germacrene, tau-cadinol, pichtosin, α-epoxy-terpenyl acetate and three other compounds were the characteristic components by MWE method; 8-acetoxy linalool, 1-hydroxy linalool, butyric acid-3-hexenyl ester, limonene oxide, 3, 16-diacetyl pseudo solasodine and thirteen other compounds were the main characteristic components via HS-SPME method. Conclusion: The above three methods combined with GC-MS can be used for the analysis of different volatile compounds in lavender and thus be feasible to establish GC-MS representation system for it.关键词：lavender;steam distillation;microwave extraction;head space-solid phase microextraction;terpenoids
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发布时间：2024-01-04 - 摘要：Objective: This study aimed at investigating the distribution of coumarins in the different parts of Cortex Daphnes. Method: The contents of daphnetin and skimmetin in the different parts of Cortex Daphnes were determined by HPLC. The contents of total coumarins in the different parts of Cortex Daphnes were determined by UV. Result: The content of daphnetin in root bark and stem bark was higher than other parts. The content of skimmetin in leaf was the highest. Both daphnetin and skimmetin in bark had higher content than in elder pith. The total coumarins were mainly distributed in bark and leaf, whose content is 4-7 times that of the elder pith. Conclusion: The root bark and stem bark in Cortex Daphnes are not only the medicinal parts in higher quality, but also the parts which are easy to peel and obtain. The leaf of Cortex Daphnes can be developed and utilized in medicine by further research.关键词：Cortex Daphnes;daphnetin;skimmetin;total coumarin;different fraction
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发布时间：2024-01-04 - 摘要：Objective: The research was conducted to study the chemical constituents of the rhizome of Matteuccia struthiopteris. Method: The constituents were separated and purified by column chromatography with normal phase silica gel and RP silica gel. Their structures were identified on the basis of physical and chemical properties, in combination of spectral data. Result: Six compounds were isolated and identified as 5, 7-dihyd-roxy-6-methyl-4'-methoxydihydroflavone(1), methoxymatteucin(2), thunberginol C(3), demethoxym-tteucinol-7-O-β-D-glucopyranoside(4), matteucinol-7-O-β-D-glucopyranoside(5), gaylussacin(6). Conclusion: All compounds were isolated from this plant for the first time, in which compounds 1 and 4 are new natural products.关键词：Matteuccia struthiopteris;chemical constituents;structure determination
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发布时间：2024-01-04 - 摘要：Objective: To develop a novel method for the determination of salicylic acid in fruits of elm. Method: The HPLC separation was performed on an Elite Hypersil C18column (4.5 mm×200 mm, 5 μm) at a flow rate of 1.0 mL· min-1 and column temperature of 30℃. Detection was monitored at 327 nm. The mobile phase was methanol-0.3% phosphoric acid (36:64). Result: Salicylic acid showed good linearity from the concentration range of 8.82 to 282.0 mg· L-1(r=0.999 8). The sample solution is steady within 8 h. The recovery was 95.23% -105.3%, RSD was 1.50%, 4.85%, 4.68%. Conclusion: The method is simple, reliable and reproducible. It can be used in the determination of salicylic acid in fruits of elm.关键词：fruits of elm;salicylic acid;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: To study the effections of Angelicae Sinensis Radix film mulching modes on the yield and component factors during the medicine formation period. Method: An experiment research was made to plant Angelicae Sinensis Radix by 6 different film mulching modes:half-covered with white film, full-covered with white film, half-covered with black film, full-covered with black film, the film side planting, and earthing the whole film planting, compared with open field cultivation. Result: Results indicated that among plastic film mulching cultivation modes, the film side cultivation was the lowest in the Angelicae Sinensis Radix emergence rate, number of plot harvested plants, single fresh root weight and yield. However, all of plastic film mulching cultivation treatments were better than that of open field cultivation. The fresh weight of single root of angelica was the heavest, 42.95 g/plants and yield was the highest up to 6 056.25 kg· hm-2 by using white film half covering mode while that was the lowest level by using open field cultivation, respectively, 28.47 g/plants and 3 087.50 kg· hm-2. Compared with CK, half-covered with white film, full-covered with white film, half-covered with black film, full-covered with black film, the film side planting, and earthing the whole film planting modes in turn was increased by 96.15%, 91.90%, 89.27%, 70.65%, 58.10%, 13.97%. Conclusion: Recommended that cultivation modes of half-covered and full-covered with black film were used in more weeds areas to plant the Angelicae Sinensis Radix in order to save the weeding labor, achieve costs saving and increase the yield; cultivation modes of half-covered and full-covered with white film were used in less weeds areas.关键词：Angelicae Sinensis Radix;film mulching modes;medicine formation period;growth;root weight
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发布时间：2024-01-04 - 摘要：Objective: The syndrome and treatment pharmacokinetics model was carried out based on the ‘real-time, dynamic, minimally invasive, in vivo’ microdialysis sampling method. Method: The intermin-gled phlegm and blood stasis syndrome coronary heart disease SD rats model was replicated by feeding with high fat diet for 7 weeks combined with left anterior descending branch coronary artery ligation. The normal group and the model group were was divided into high and low dose group with 6 rats in each group, the extract of Danshensu dosage was respectively 25.3, 50.6 mg· kg-1. The pharmacokinetics study was carried out on normal and coronarg heatt disease(CHD) rats by intragastric administration of different dose. The Danshensu concentration of microdialysis samples were detected with HPLC and the pharmacokinetic parameters was obtained using noncompartmental model. Result: The results showed that serum total cholesterol(TC), triglyceride(TG), creatine phosphokinase(CK) in the model group increased, blood viscosity of high, medium and low shear also increased. The results had highly statistically significant differences compared with the normal group(P<0.01).The results showed that Danshensu peak concentration increases, but without statistical significance, the peak time shorten, area under the curve(AUC) and area under the first moment curve(AUMC) increases in CHD rats comparing with the normal group, the results were statistically significant(P<0.05). Conclusion: This study can be the reference for the similar research mode.关键词：phlegm and blood stasis type coronary heart disease;blood microdialysis;danshensu;syndrome and treatment pharmacokinetics
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发布时间：2024-01-04 - 摘要：Objective: To investigate small intestine absorption characteristics in rats of glucoraphenin in Raphani Semen extract. Method: Taking in situ rat single-pass intestinal perfusion model, effects of different drug mass concentrations (0.02, 0.05, 0.1 g· mL-1) on intestinal absorption characteristics of glucoraphenin was investigated, and calculated their absorption rate constant (Ka) and apparent absorption coefficient (Papp).HPLC was employed to determine the content of glucoraphenin with mobile phase of 0.1% phosphoric acid solution-acetonitrile (87:13) and detectopm wavelength at 225 nm. Result: Glucoraphenin had the best stability in K-R test solution with pH of 5.12.In Raphani Semen extract with different concentrations, Ka of glucoraphenin in small intestine of rats were (7.750±1.744)×10-3, (7.204±1.045)×10-3, (7.148±0.837)×10-3 min-1, Papp were (1.519±0.507)×10-3, (1.504±0.617)×10-3, (1.592±0.345)×10-3 cm· min-1, these data had no significant changes. Conclusion: Glucoraphenin was easy-to-absorped component, its absorption mechanism in small intestine of rats was passive diffusion.关键词：Raphani Semen;glucoraphenin;in situ single-pass intestinal perfusion model;intestinal absorption characteristics;sulfur glycosides
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发布时间：2024-01-04 - 摘要：Objective: To reveal the therapeutic effects of water-solubility alkaloid after using of Aconitum carmichaelii on heart-failure cell model by studying its influence on myocardial cells' membrane ATP enzyme and relative ions. Method: Culture the myocardial cells, gotten from the neonatal rats, for 5 days until the cells became mature, then nembutal (0.8%) was used to establish the heart-failure cell model for 5 min administrate them with 3 concentrations of A. carmichaelii water-solubility alkaloid (0.01, 0.02, 0.04 g· L-1) and deslanoside injection (4 mL·L-1) determine the vitality of the myocardial cells' membrane ATP enzyme and the concentration of relative ions 90 min later. Result: Compared with the blank control group, in the model group the survival rates for cells were restrained, the concentrate of Na+, Mg2+ and the activity of Ca2+-ATP enzyme, Ca2+-Mg2+-ATP enzyme was decreased. On the contrary, the concentrate of K+, Ca2+ and the activity of Na+-K+-ATP enzyme was increased. Deslanoside injection (4 mL·L-1) and A. carmichaelii water-solubility alkaloid (1.25×10-3, 2.50×10-3, 5.00×10-3g·kg-1) could increase the the survival rates of heart-failure cells and the concentrate of Na+, Mg2+, and also enhance the activity of Ca2+-ATP enzyme, Ca2+-Mg2+-ATP enzyme. Conversely, all the administrated groups decreased the concentrate of K+, Ca2+ in the myocardial cells and inhibited the activity of Na+-K+-ATP enzyme. Conclusion: Water-solubility alkaloid of A. carmichaelii has the therapeutic effect on heart-failure cells.关键词：heart failure;water-solubility alkaloid of Aconitum carmichaelii;Myocardial cells;enzyme;ions
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发布时间：2024-01-04 - 摘要：Objective: To observe effects of Artemisiae Argyi Folium aqueous extract and its ferment substance on candida albicans vaginitis in mice. Method: The Candida albicans vaginitis model was induced by the vagina in mice which were vaccinated 1×107cfu/mL C. albicans suspension 20 μL. The model mice were randomly divided into control group, model group, Artemisiae Argyi Folium aqueous extract group(20 g· L-1), Artemisiae Argyi Folium ferment substance (AAFS) groups with different doses (10, 20, 40 g· L-1) and fluconazole group (2 g· L-1), 15 mice each group. The mice in each group were given therapeutic drugs by vagina vaccination for 7 d, once per day. The candida albicans and epithelial cells in vaginal lavage liquid were determined by microscopic examination. HE staining observed pathological changes of vaginal tissue. Result: The candida albicans infection was found in model group, but not in blank control group. Compared with the model group, AAFS with different doses and fluconazole could remarkably increase negative-converting rate of candida albicans and epithelial cells, decrease CFU number, and reduce the damage of vaginal tissue at 5 or 8 day after the treatment (P<0.05, P<0.01), and AAFS with middle and high doses had more remarkable effects at 8 d after administration(P<0.01), but Artemisia Argyi aqueous extract group had no difference on above indexes. Conclusion: Compared with Artemisia Argyi aqueous extract, AAFS had significant therapeutic effects on C. albicans vaginitis in mice.关键词：Artemisiae Argyi Folium;ferment;Candida albicans;vaginitis
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Danggui Buxue decoction (DGBXD) on phenylhydrazine hydrochloride-induced oxidative hemolysis and investigate its mechanism related to antioxidant capacity in mice. Method: The mouse model of oxidative hemolysis was established by intraperitoneal injection of phenylhydrazine hydrochloride. Mice were divided into normal group, model group, DGBXD high, medium and low dose groups (10, 5, 2.5 g· kg-1· d-1) and prednisone treatment group (0.005 g· kg-1· d-1). Then, the effects of DGBXD on model mice were observed. The contents of hemoglobin(Hb) and red cell malondialdehyde (MDA) were measured. The numbers of red blood cells (RBC) and activity of superoxide dismutase (SOD) was also determined and the body weight was analyzed. Result: Compared with the control group, the number of RBC, level of Hb and activity of SOD were significantly decreased in the model group (P<0.01 or P<0.05), the content of MDA in RBC was markedly increased in mice model with oxidative hemolysis (P<0.05). Compared with the model group, DGBXD could increase the number of RBC and level of Hb, decrease the content of MDA and raise the activity of SOD in RBC (P<0.01 or P<0.05). Conclusion: DGBXD has good efficacy in treating drug-induced oxidative hemolysis in mice. Its mechanism might be related to the promotion of hematopoietic function, enhancing red cell antioxidant capacity and protection of erythrocytes against oxidative damage.关键词：Danggui Buxue decoction;oxidative hemolysis;red blood cell;hemoglobin;superoxide dismutase;malondialdehyde
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发布时间：2024-01-04 - 摘要：Objective: To study antioxidant activity of the different part of Elaeagnus bockii in vitro and in vivo, and evaluate the value of development and utilization of aboveground parts of elaeagnus and anti-atherogenic effects. Method: Evaluation of its antioxidant activity in vitro were based on effect of copper ion-induced low density lipoprotein(LDL)oxidation and· OH and O2 . scavenging at low, middle and high concentration(5, 10, 20 g· L-1). The model KM mice were induced by injecting subcutaneous 5% D-galactose for 8 weeks. Intragastric administration for 14 days and then the orbital blood was taken to test total antioxidant capacity (TAC), superoxide dismutase(SOD), glutathione peroxidase(GPX) antioxidant enzymes activities and malonyldialdehyde(MDA) content in serum(2, 5, 10 g· kg-1). Result: The rate of free radical scavenging for O2 . at different concentration of Root and rhizome(UP) part were (58.26±6.14)%, (68.21±3.47)%, (74.67±4.10)%, the radical scavenging for· OH were(48.24±6.24)%, (78.21±7.24)%, (90.17±6.58)%. The free radical scavenging rate of the ground part was higher than that of underground parts. Extracts from different parts significantly increased oxidation of delay time(Lag time) and the maximum rate of oxidation time (Tmax). The result was consistent with reduced MDA formation induced by copper ion. Serum TAC, SOD, GPX activity of antioxidant enzymes could be increased obviously, and decreased the content of MDA in serum of mice. Conclusion: Different part of E. bockii has good antioxidant activities in vitro and in vivo, significantly increase LDL and serum antioxidant capacity. Antioxidant activity of elaeagnus on aerial parts is significantly superior to the underground parts, which can be developed from above ground parts of elaeagnus for its great development value.关键词：Elaeagnus bockii;fruit;root and rhizome;antioxidant activity;Low density lipoprotein
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Xuefu Zhuyu decoction on ventricular remodeling and cardiac function in the rabbits with coronary heart disease at the stage of blood stasis syndrome and discuss its therapeutic mechanisms. Method: Sixty healthy rabbits were randomly divided into control group, sham-operation group, model group, and Xuefu Zhuyu decoction low-dose, middle-dose, high-dose groups six groups with ten rabbits in each group. Left coronary artery was implanted constrictor ring to establish the model of coronary heart disease at the stage of blood stasis syndrome. The sham-operation group was just opened the chest but not implanted the constrictor ring. On the basis of the model group from the first day of the twelfth-week after operation, the low-dose, middle-dose, high-dose groups were respectively intragastric administrated(ig) with Xuefu Zhuyu decoction according to 10, 20, 40 g· kg-1, once a day for continuous twenty-one days while the control group, the model group and the sham-operation group were ig the same volume normal saline, then to record left ventricular end diastolic volume(LVEDV), left ventricular end systolic vulume (LVESV), left ventricular end diastolic diameter (LVDd), left ventricular end systolic diameter (LVDs), stroke volume (SV), left ventricular ejection fraction(LVEF), left ventricular weitht index(LVWI), right ventricular weitht index(RVWI).To examine the levels of atrial natriuretic peptide(ANP)and angiotensin Ⅱ(AngⅡ)by ELISA and the expression of α-myosin heavy chain(α-MHC)mRNA and β-myosin heavy chain(β-MHC) mRNA by RT-PCR. To test nicotinamide adenine dinucleotide phosphate oxidase(NADPH oxidase) activity by revivification test of cytochrome C. Result: Compared with the control group, in the model group the levels of AngⅡ increased significantly(P<0.01), NADPH oxidase activity increased significantly(P<0.01), the expression of α-MHC mRNA reduced significantly(P<0.01)while the expression of β-MHC mRNA increased significantly(P<0.01), meantime, LVEDV, LVESV, LVWI and RVWI all increased (P<0.05), LVDs, SV and LVEF all reduced significantly(P<0.01). Compared with the model group, in the low-dose group the level of ANP reduced(P<0.05). In the middle-dose group NADPH oxidase activity reduced(P<0.05)and the levels of AngⅡ and ANP reduced significantly(P<0.01).In the high-dose group NADPH oxidase activity, the levels of AngⅡ and ANP all reduced significantly(P<0.01). LVEDV, LVESV, LVWI, RVWI, LVDs, SV and LVEF in both the middle-dose group and the high-dose group were improved significantly than those in the low-dose group(P<0.05 or P<0.01). Conclusion: Xuefu Zhuyu decoction could recover the level of AngⅡ in the rabbits with coronary heart disease at the stage of blood stasis syndrome and regulate NADPH oxidase activity, the levels of ANP, α-MHC and β-MHC by AngⅡ mediating, which might be the part of its therapeutic mechanisms.关键词：Xuefu Zhuyu Decoction;coronary heart disease;blood stasis syndrome;angiotensin Ⅱ
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antimicrobial fractions of Galla Chinensis. Method: The minimum inhibitory concentrations (MICs) of portions of petroleum ether, chloroform, ethyl acetate, n-butanol, ethanol and water of Galla Chinensis against five bacterias and Candida albicans were determined by agar dilution method. The data were analyzed using statistical software SPSS 17.0, and multifactor analysis of variance (ANOVA) and independent samples t test were used to compare the differences between 2 active fractions of ethyl acetate, n-butanol against same bacterial species, and between Clotrimazole and ethyl acetate fraction of Galla Chinensis against C. albicans. Result: The 3 portions of petroleum ether, chloroform and ethanol of Galla Chinensis had no effect on bacteria and C. albicans, and water fraction only had weak activity against S. aureus, with MICs 733.69 g·L-1, n-butanol fraction only had antibacterial activity, with MICs ranging from 2.06 g· L-1 to 32.96 g· L-1. However, ethyl acetate fraction of Galla Chinensis had strong antibacterial and antifungal activities, with MICs ranging from 2.88-23.07 g· L-1 and 0.36-27.28 g· L-1, respectively. The ethyl acetate fraction and n-butanol fraction of Galla Chinensis had higher activity against Gram-positive isolates than Gram-negative isolates (P<0.01, P<0.05), and the two active-fractions had similar antibacterial activity against sensitive and resistant bacteria. The ethyl acetate fraction of Galla Chinensis had strong effect on C. albicans from sensitive and resistant clinical isolates, with MICs ranging from 0.36 g· L-1 to 0.72 g· L-1, but the resistance rate of Clotrimazole was 14.29% (2/14). Conclusion: Antibacterial and antifungal active fraction of G.chinensis was ethyl acetate fraction, which had broad-spectrum antimicrobial activity so to make it a potential candidate as antimicrobial agent to treat infectious diseases.关键词：Galla Chinensis;Active fraction;in vitro antibacterial activity;in vitro antifungial activity
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of breviscapine(Bre) on serum glucose, serum lipid and insulin resistance in type 2 diabetes mellitus rats with insulin resistance and find its mechanism. Method: Experimental diabetic insulin resistance was duplicated in rats by feeling on high-fat and high-carbohydrate diet four weeks plus injecting low-does streptozotocin(STZ, 40 g· L-1).Then the rats whose free blood glucose (FBG) level was equals to or surpasses 16.7 mmol· L-1 were selected and divided into model group of Bre, the low does group, and the high does group. The medication was given for 14 days. FBG, fasting insulin(FINS), free fatty acids(FFA), triglycerides(TG), total cholesterol (TC) were measured and calculated the insulin resistance index(HOMA-IR) after 14 days.The pancreas, myocardial tissues were stained by HE so as to observe its morphologic changes. Result: FBG, FINS, TG, TC, FAA, HOMA-IR increased in the model group as compared with those in nomal group. FBG, FINS, TG, TC, FAA, HOMA-IR reduced in the Bre group compared with those in model group. Curative effect of Bre-H group was better than Bre-L group (P<0.05).Through HE-stain in model group the organizational structure of the pancreas and myocardial tissues was abnormal. The pathologic changes in different does Bre groups were significantly improved. Conclusion: Bre can reduce levels of blood glucose, regulate components and content of blood lipids, and improve the insulin resistance.关键词：breviscapine;type 2 diabetes mellitus;insulin resistance
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发布时间：2024-01-04 - 摘要：Objective: To study the antidepressant activity and mechanism of extracts from Artocarpus styracifolius Pierre (AS). Method: Antidepressant activity was tested by tail suspension test, force swimming test and reserpine model of different polar extracts from AS, and serotonin (5-HT) content was determined by fluorescence spectrophotometry. AS was given by intragastric administration for 10 days (fluoxetine group 50 mg· kg-1; each AS group 200 mg· kg-1). Result: The different polar extracts of AS could shorten immobility time in tail suspension test and force swimming test on mice(P<0.05 or P<0.01). Action of n-butanol extract of AS could have an antagonistic action on reserpine caused drop of body temperature and blepharoptosis and improved 5-HT content in hippocampal convolution and corpus striatum on mice(P<0.05 or P<0.01). Conclusion: Extracts of AS had antidepressant activity, and activity part may be n-butanol extract.关键词：Artocarpus styracifolius;antidepressant;extract;serotonin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of ligustrazine microemulsion gel on the expression of ctokine in peritoneal fluid and antioxidase in the ahension tissue in rats with peritoneal adhesion. Method: Seventy SD rats were randomly divided into 7 groups:normal control group, model group, blank microemulsion gel groups (5 mL· kg-1), ligustrazine group(15 mg· kg-1) and low-, medium-and high-dose ligustrazine microemulsion gel groups(in the dose of 7.5, 15, 30 mg·kg-1 respectively).Except the normal control group, the rats in other groups were induced ankylenteron. Intraperitoneal injection of saline was administered to the rats in the normal control group and the model group, and ligustrazine groups were given ligustrazine injection to abdominal cavity.The treatment lasted 10 days.In the low-, medium-and high-dose ligustrazine microemulsion gel groups, the microemulsion gel were scribbled evenly on the wound by syringe in the abdominal cavity after modeling.On the 11th day after surgery, the peritoneal fluid and the adhesion tissue of each group were collected.The levels of interleukin-18(IL-18), interleukin-6(IL-6) and tumor necrosis factor-a(TNF-a) in peritoneal fluid were determined by enzyme linked immunosorbent assay and the contents of superoxide dismutase (SOD), nitric oxide synthase (NOS), glutathione(GSH), Malondialdehyde(MDA), nitric oxide(NO), catalase(CAT), glutathione peroxidase(GPx) in the adhesion tissue were measured by kits.Grades of intestinal adhesion were ranked by macroscopic observation.The intestine tissue were adopted to observe the change of pathomorphology. Result: Comared with the contol group, ligustrazine microemulsion gel could significantly relieve the experimental intestinal adhesion and decreased the levels of IL-18, IL-6 and TNF-α in peritoneal fluid obviously(P<0.05, P<0.01).The activity of MDA, NO, NOS in the adhesion tissue were reduced significantly(P<0.05, P<0.01), but the activity of CAT, GPx, GSH, SOD were increased markedly(P<0.05, P<0.01)in the low-, medium-and high-dose ligustrazine microemulsion gel groups. Pathomorphology investigation showed that small intestine injury was restored in low-, medium-and high-dose ligustrazine groups. Conclusion: Ligustrazine microemulsion gel can effectively prevent the formation of postoperative ankylenteron by inhibiting the expression of inflammatory cytokines and be correlated with anti-intestine tissue peroxidation.关键词：ligustrazine;microemulsion gel;postoperational intestinal adhesion;interleukin-6;interleukin-18;tumor necrosis factor-α;peroxidation
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the prevention and treatment action of Shashen Maidong decoction and thus explore the possible mechanisms. Method: One hundred forty-one SD rats were randomly divided into five groups, control group, model group, Shashen Maidong decoction 10.3 g·kg-1 prevention group, Shashen Maidong decoction 10.3 g·kg-1 treatment group, and dexamethasone 10.4 mg·kg-1 treatment group. The rats model of radiation pneumonitis was established by exposeing rats to the chest X-ray except control group. The prevention group was given Shashen Maidong decoction one week before modeling, while the corresponding drug solutions of other four groups were administrated from the second day after modeling respectively once per day. Rats were anesthetized by intraperitoneal injecting 10% chloral hydrate. The blood was collected. The levels of plasma interleukin-6(IL-6), transforming growth factor-β1(TGF-β1) and tumour necrosis factor-α(TNF-α) were measured by ELISA at 2, 4, 6 weeks seperately after modeling. Result: Compared with control group, IL-6, TGF-β1 and TNF-α increased in model group (P<0.01) at 2, 4, 6 weeks. Compared with model group, the levels of serum IL-6, TGF-β1, TNF-α in Shashen Maidong decoction prevention group, Shashen Maidong decoction group and dexamethasone treatment group were significantly lowered (P<0.01, or P<0.05). The levels of IL-6, TGF-β1, TNF-α in Shashen Maidong decoction prevention group were lower than Shashen Maidong decoction group and dexamethasone treatment group (P<0.01), and in dexamethasone treatment group which was higher than that of Shashen Maidong decoction treatment group (P<0.01). Conclusion: The Shashen Maidong decoction may inhibit the inflammation of alveolar and improve the process of pulmonary fibrosis in radiation peumonitis by reducing the levels of IL-6, TGF-β1, TNF-α.关键词：Shashen Maidong decoction;radiation pneumonitis;interleukin-6;transforming growth factor-β1;tumour necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: This study aimed to investigate the regulation roles of lycium barbarum polysaccharides (LBP) on cytokines in type 2 diabetic (NIDDM) rats. Method: High-fat and high-sugar diet in combination with low-dose injection of streptozotocin(STZ) was used to prepare type 2 diabetic (NIDDM) rat model. Forty diabetic immune model rats were randomly selected to divide into five groups:model group, low dose of LBP(100 mg· kg-1) intervention group, medium dose of LBP (200 mg· kg-1) intervention group, high dose of LBP (400 mg· kg-1) intervention group, glibenclamide intervention group (positive drug group). Rats in similar weight fed with basal diet were used as normal control group. Western bolt assay was used to detect the effect of LBP on the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α) in type 2 diabetic rats' spleen tissue. Result: Compared with the normal control group, the expression of IL-1β, IL-6, TNF-α in the spleen tissue of NIDDM rats was significantly increased. After 4 weeks' intervention of different doses of LBP, the expression of IL-1β, IL-6, TNF-α in diabetic rats' spleen tissue in each treatment group significantly decreased. Conclusion: LBP may regulate the immune function of type 2 diabetic rats by inhibiting the expression of IL-1β, IL-6 and TNF-α.关键词：total lycium barbarum polysaccharide;diabetic rats;interleukin-1β;interleukin-6;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Bushen Huatan decoction on tumor necrosis factor (TNF-α) and interleukin-6(IL-6) in ovariectomized rats and to explore the therapeutic mechanism of the drugs. Method: Female SD rats were divided into five groups:model group(A), sham operation group(B), livial group(C), Bushen Huatan decoction low dose group(D) and high dose group(E). Except for group B, all rats were ovariectomized. Drug treatment began at three months after the operation. Group C was treated with livial at the dose 0.23 mg· kg-1, group D and E were treated with the low dose 9.4 g· kg-1 the large dose 18.8 g· kg-1 and Bushen Huatan decoction respectively A, B group recewed the same volume of physiological saline. After 8 weeks intragastric administration by once a day, the rat's bone mineral density was measured by dual energy X-ray absorptionmetry. Levels of TNF-α and IL-6 in bone tissue were determined by using immunohistoehemistry techniques. Result: Bushen Huatan decoction can evidently increase bone density as well as the trabecular bone volume and density, and decrease the levels of TNF-α and IL-6 in bone. Conclusion: Bushen Huatan decoction can effectly prevent osteoporosis in female rats induced by ovariectomy, and the mechanism maybe lowing levels of TNF-α and IL-6 in bone tissue.关键词：Bushen Huatan decoction;osteoporosis;tumor necrosis factor-α;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: The aim of the study was to explore the effect of Chinese medicine in combination with rehabilitation training on extremity function and life quality in cerebral infarction patients. Method: One hundred and sixty cases with acute cerebral infarction were randomly divided into control group and experience group. All the patients received routine western medical treatment and early rehabilitation training. Experience group was given nourishing Qi and activating blood circulation medicine therapy. The course of treatment was 1 month. Fugl-meyer assessment (FMA) and Spitzer scales were used to evaluate extremity function and life quality. Meanwhile, the change of blood rheology indexwas compared between two groups. Result: The effective rates in experience group and control group were 91.3% and 75.0% (P<0.05), respectively;after treatment, FMA and Spitzer scores in experience group were much higher than those in control group (P<0.01), and blood rheology index of experience group was superior to control group (P<0.01). Conclusion: Chinese medicine in combination with rehabilitation training can greatly improve the extremity function and life quality of cerebral infarction patients.关键词：cerebral infarction;early stage rehabilitation;extremity function;life quality
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Huoxin prescription on therapeutic efficacy of angina pectoris of coronary heart disease and serum level of vascular endothelial growth factor(VEGF). Method: Sixty patients with angina pectoris were randomly assigned into traditional Chinese medicine(TCM) group (31 patients) and control group (29 patients). All the patients involved were treated with western medicine, and the patients in TCM group were given the Chinese medicine, Huoxin prescription, in addition. Then we observed the therapeutic efficacy of the TCM syndrome and angina pectoris, left ventricular ejection fraction (LVEF) and serum level of VEGF. Result: Compared with the control group, TCM syndrome score of the patients in TCM group was further decreased(P<0.05), and the total efficiency of TCM syndrome and angina pectoris were higher(P<0.05). Compared with before treatment, the LVEF and serum level of VEGF of the patients in TCM group were increased(P<0.05, P<0.01), and there were significant differences between the two groups after treatment(P<0.05). Conclusion: The symptoms of the patients in TCM group were further improved by treating with Huoxin prescription, and the therapeutic efficacy was further increased as well. Simultaneously, the LVEF and serum level of VEGF of the patients in TCM group were increased by treatment of Huoxin prescription.关键词：Huoxin prescription;angina pectoris of coronary heart disease;therapeutic efficacy;vascular endothelial growth factor
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发布时间：2024-01-04 - 摘要：Objective: To study the Wuwei tang combined with gemcitabine and cisplatin in the treatment of non-small cell lung cancer with postoperative adjuvant chemotherapy. Method: Eighty-four patients underwent surgical treatment of non-small cell lung cancer were divided into observation group and control group in accordance with the principle of randomization group, 42 cases in each group, two groups of gemcitabine and cisplatin scheme is adopted for postoperative adjuvant chemotherapy and 28 days for a cycle, a total of four cycles, observation group of combined therapy with Wuwei tang, daily 1 agent, treatment course for four months. Observe two groups of patients after chemotherapy in the near future curative effect and drug toxicity reaction. Result: The observation group of syndromes was objective response rate and total effective rate were better than control group(P<0.05), drug toxicity reaction significantly lower than control group (P<0.05). Conclusion: Wuwei tang combined with gemcitabine and cisplatin for non-small cell lung cancer with postoperative adjuvant chemotherapy, can significantly enhance chemotherapy curative effect, reduce drug toxicity reaction.关键词：Wuwei tang;gemcitabine;cisplatin;non-small cell lung cancer;postoperative adjuvant chemotherapy
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发布时间：2024-01-04 - 摘要：Objective: To discuss the influence of severe acute pancreatitis(SAP)intestinal mucosal barrier function and inflammatory factor by taking Yidan decoction oral and enema. Method: Eighty-three patients were randomly divided into control group (42 cases) and Chinese medicine group(41 cases) according to digital method.Patients of the two groups received conventional western medicine treatment, which referred to diagnosis and treatment of severe acute pancreatitis' guidance, octreotide acetate injection, 0.6 mg dissolved in 50 mL saline, picked up intravenous infusion pump, pumping continuous 24 hours.Omeprazole sodium for injection, 40 mg/day, intravenous drip.Imipenem and cilastatin sodium for injection, 1.0 g/time, 2 does/day, intravenous drip.Based on the treatment of control group, patients in Chinese medicine group added Yidan decoction taken orally combined with enema.Course of treatment in two groups were both 14 days.Occurrence and conversive rates of acute respiratory distress syndrome(ARDS) and multiple organ dysfunction syndrome(MODS) were recorded.And time of stomachache, remission of abdominal distension, restoration of gurgling sound and the first time of ventilation defecation were also recorded.At the zeroth, third and seventh day after treatment, acute physiology scores and acute physiology and chronic health scores(APACHEⅡ) were made.Levels of D-lactic acid in plasma, diamine oxidase (DAO), endothelin(ET), nitric oxide(NO) and malonaldehyde(MAD) were monitored.Levels of tumor necrosis factor-α(TNF-α), interleukin-1β((IL-1β), interleukin-6(IL-6) and interleukin-10(IL-10) were monitored. Result: At the third and seventh day after treatment, levels of serous D-lactate, DAO, ET, NO and MDA in Chinese medicine group were lower than those in control group of corresponding period(P<0.01).And levels of TNF-α, IL-1β and IL-6 were lower than those in control group, but level of IL-10 was higher than in control group(P<0.01).Time of stomachache, remission of abdominal distension, restoration of gurgling sound and the first time of ventilation defecation were all less than those in control group(P<0.01).At the seventh and fourteenth day after treatment, score of APACHE in Chinese medicine group was lower than in Chinese medicine group(P<0.01).For chinese medicine group, occurrence rate of ARDS was 14.63% lower than 38.09% in control group.Besides occurrence rate of MODS was 12.19% lower than 30.95% in control group(P<0.05). Conversion rate of operation in Chinese medicine group was 9.75% lower than 19.05% in control group, but there was no statistical significance from the differences between two groups. Conclusion: Yidan decoction taken orally combined with adjuvant therapy of enema in treating SAP, can reduce occurrence rate of ARDS and MODS, promote recovery of patients with SAP, and its mechanism of action may be related to regulation of inflammatory factors, reducing inflammatory response, ameliorating microcirculation disorder of intestine and protecting integrity of intestinal mucosal barrier.关键词：severe acute pancreatitis;Yidan decoction;function of intestinal mucosal barrier;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: Discuss near-term and forward analgesic effect of Jiawei Shaofu Zhuyu decoction combined with moxibustion therapy and influence to hemorheology, prostaglandin E2 (PGE2)and prostaglandin 2α (PGF2α) in treating primary dysmenorrhea(PD). Method: One hundred and eight patients who had by random number table were divided into control group (53 cases) and observation group (55 cases).Period of this study was divide into introduction period(the first menstrual cycle, D1), cure period(the second, third and fourth menstrual cycle, D2-4) and follow-up period(the fifth and sixth menstrual cycle, D5 and D6). Patients in two groups were received aspirin effervescent tablets in introduction period and cure period, 0.5 g/time, 3 times/day, taken orally necessarily visual analogue scale(VAS≥5 scores). In cure period, patients in control group received aspirin effervescent tablets, 0.5 g/time, 3 times/day, and used at the third day before menstruation and stopt at the first day after menstruation.Patients in observation group received jiawei Shaofu Zhuyu decoction(taken orally), 1 dose/day, and used at the fifth day before menstruation and stopt at the first day after menstruation.moxibustion therapy used at the third day before menstruation and stopt at the second day after menstruation.Recording the degree of dysmenorrhea score(VAS), duration of dysmenorrhea and symptoms of dysmenorrhea from D1 to D6.Before and after treatment, hemorheology was detected.And levels of serous of PGF2 and PGE2α were detected. Result: At the point-in-time of D2, compared with the point-in-time of D1, scores of VAS, duration and symptoms of dysmenorrhea in two groups decreased(P<0.01), and at the point-in-time of D3 and D4, there was a decreasing trending of scores of VAS, duration and symptoms of dysmenorrhea in two groups.At the point-in-time of D5 and D6, scores of VAS, duration and symptoms of dysmenorrhea in two groups increased, and which were higher than those in two groups of the point-in-time of D4(P<0.01).And at the point-in-time of D2 to D6, scores of VAS, duration and symptoms of dysmenorrhea in observation group were lower than those in control group(P<0.01).After treatment, amelioration of whole blood viscosity(low shear and high shear), plasma viscosity, whole blood reduced viscosity, fibrinogen and hematokrit in observation group were superior to those in control group(P<0.01).And levels of PGE2 in observation group was higher than in control group, but levels of PGF2α and PGF2α/PGE2were lower than those in control group (P<0.01). Conclusion: Jiawei Shaofu Zhuyu decoction combined with moxibustion therapy had significant near-term and forward analgesic effect in treating primary dysmenorrhea, the mechanism of action may be related to improving blood circulation, adjusting prostaglandin and giving play to analgesia effect.关键词：primary dysmenorrhea;jiawei Shaofu Zhuyu decoction;moxibustion therapy;hemorheology;prostaglandin E2;prostaglandin 2α;combined effect
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发布时间：2024-01-04 - 摘要：Objective: Observe the clinical effects of Bushen Qiangdu decoction on cure of spinal joint disease(SpA) added western medicine treatment and the serum interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α). Method: Fifty-eight patients were divided into western medicine group for (28 cases)and observation group for (30 cases).For patients in control group took celecoxib capsules, 0.2 g/time, 1 does/day, stopped as the pain control.Besides they took leflunomide tablets, 20 mg/time, 1 does/day, sulfasalazine enteric-coated tablets, 1.0 g/time, 2 does/day.On the basis of western medicine group treatment, observation group used Bushen Qiangdu decoction for dialectical add and subtract treatment.Both groups' treatment courses were four months.Conducted PAP disease activity index(BASDAI) Bath ankylosing spondylitis functional index(BASFI), spinal pain Likert four level score and overall assessment four level score from doctor before and after treatment.Graded joint pain, joint swelling and attachment points pain for pre-therapy and post-treatment.Test erythrocyte sedimentation rate (ESR), C-reactive protein(CRP), interleukins-6(IL-6) and tumor necrosis factor(TNF-α). Result: After treatment, BASDAI, BASFI, Likert and doctor's overall assessment score for observation group were lower than those data in western medicine group(P<0.01).Patients in observation group'scores of joint pain, joint swelling and attachment points pain were inferior to those data in western medicine group(P<0.01).The level of ESR, CRP, IL-6 and TNF-α for observation group were below to the level in western medicine group after treatment (P<0.01).There were 14 patients(50%) reached ankylosing spondylitis activity score-50 (ASAS50) in western medicine group and 19 patients(63.3%) in observation group.ASAS50 in observation group was higher than that in western medicine group.But there was no obvious statistically difference. Conclusion: Syndrome differentiation treatment of Bushen Qiangdu decoction combined with western medicine in treating spondyloarthropathy can ameliorate pain, swelling et acute symptoms.It was able to improve joint and spine dysfunction and had function of anti-inflammatory and immunoregulation.It can western medicine the disease activity.关键词：spinal joint disease;Bushen Qiangdu decoction;interleukin-6;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To discuss curative effect of big plant rhodiola capsule to treat unstable angina(UA) and the influence of hemorheology and hematoblastic activation function. Method: Ninety-two patients were randomly divided into control group (46 cases) and observation group (46 cases) by the sequence of being in hospital. Patients in control group received aspirin enteric-coated tablets, 100 mg/time, metoprolol succinate sustained-release tablet, 47.5 mg/time, 1 does/day, taken in the morning, enalapril maleate tablets, 10 mg/time, 2 does/day, atorvastatin calcium tablets, 20 mg/time, 1 does/day, taken after dinner. For patients who were hard to relieve himself with angina, took nitroglycerin tablets, 1 piece/time, keeping under tongue when necessary. Patients in observation group took big plant rhodiola capsule based on the treatment of control payients, 4 pieces/time, 3 does/day. Both treatment courses continued 8 weeks. Test electrocardiogram before and after treatment. Record angina pectoris' attack frequency and dosage of nitroglycerin tablets during observation time. Give a mark of seattle angina questionnaire(SAQ) before and after treatment. Detect hemodynamic indexes and the level of platelet-α membrane protein particles(GMP-140), thromboxane B2(TXB2), false von willebrand factor(vWF), plasminogen activator inhibitor 1(PAI-1) before and after treatment. Result: After treatment, levels of GMP-140, TXB2, vWF and PAI-1 in observation group were lower than those in control group(P<0.01).Amelioration of whole blood viscosity(low shear and high shear), blood reduced viscosity, plasma viscosity, fibrinogen and platelet aggregation rate in observation group were superior to those in control group(P<0.01).Attack times of angina every week and dosage of nitroglycerin tablets in observation group were both less than those in control group(P<0.01).SAQ scores in observation group was higher than in control group(P<0.01). And the total effective rate of electrocardiogram curative efficacy in observation group was 93.48% superior to 78.26% in control group(P<0.05). Conclusion: Based on the conventional western medicine treatment, Dazhu Hongjingtian capsules can reduce attack times of angina and improve SAQ scores, the curative efficacy may be related to ameliorate patients'hemorheology and functions of platelet, and inhibit thrombogenesis.关键词：unstable angina;Dazhu Hongjingtian capsules;seattle angina questionnaire scores;platelet activation function;thrombogenesis
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发布时间：2024-01-04 - 摘要：Objective: The observe taken orally the Yiqi Huadu decoction which combined with cinobufagin Bladder irrigation to prevent and cure postoperative recurrence of superficial bladder cancer to survey the influence of patients' cellular immune function and vascular endothelial growth factor(VEGF). Method: Ninety patients were randomly divided into western medicine group (45 cases) and observation group (45 cases) by random number method. Both group adopt urinary bladder tumor resection (TUR-BT).After the surgery, the patients in western medicine group took pirarubicin hydrochloride, 30 mg/time, once a week, by bladder perfusion, 8 times in total.Since then, 1 time in a month, 10 times in total.Treatment courses were 12 months.Use Huachan hormone injections (30 mL/time)and pirarubicin hydrochloride (30 mg/time)by bladder perfusion for observation group.The methods and treatment were the same as the western medicine group.In the meantime, observation group patients added Yiqi Huadu decoction, one does a day in the first three months.Since then, took a potion in interval one day.The total treatment courses were 12 months.Using B ultrasound and cystoscope et to detect patients' relapse within a year.Adopt flow cytometry instrument method test the level of peripheral blood T lymphocyte subsets(CD3+, CD4+, CD8+和CD4+/CD8+)and natural killer cells(NK) at the third, sixth and twelfth month before and after treatment.Detect the level of vascular endothelial growth factor(VEGF) and record quality of life (KPS) score at the third, sixth and twelfth month before and after treatment.Record adverse reactions, such as nausea, vomiting, abdominal pain, diarrhea, etc.Besides monitor the liver function and blood routine. Result: During one year after operation, numbers of relapsing cases in observation group were 7 and the rate was 15.56%, numbers of relapsing cases in western medicine group were 15, and the rate was 33.33%, and rate of relapsing cases in observation group was lower than in western medicine group(P<0.05).At the sixth mouth after treatment, levels of CD3+, CD4+, CD4+/CD8+ and NK in observation group were higher than those in western medicine group(P<0.01), and level of CD8+ was lower than in western medicine group(P<0.01).After three mouths of treatment, compared with the time before treatment, score of KPS in observation group decreased(P<0.05), which in western medicine group was not significant.After three, six and twelve mouths of treatment, scores of KPS in observation group were higher than those in western medicine group(P<0.05, P<0.01).After six and twelve mouths of treatment, levels of serous VEGF in observation group were lower than those in western medicine group(P<0.01).Cumulative happened of adverse reactions in western medicine group were 26 cases, and the rate was 57.7%, cumulative happened of adverse reactions in observation group were 15 cases, and the rate was 33.3%, which in observation group were lower than those in western medicine group(P<0.05). Conclusion: Cinobufacini bladder perfusion combined with oral Yiqi flower and reduce the recurrence of bladder cancer after operation, reduce the adverse reaction induced by chemotherapy, improve the quality of life, and its mechanism may be related to improving cellular immune function of patients, reduce the level of serum VEGF.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effect of Guipi decoction compared with Fluoxetine for depression patients with Heart-spleen deficiency. Method: Sixty cases of patients were randomly divided into Guipi decoction group and the Fluoxetine group with 30 cases in each group. Patients were given with Fluoxetine (20 mg, once a day) in the Fluoxetine group and Guipi decoction was given (half of one decoction, twice a day) in the treatment group. The treatment course was 6 weeks. Hamilton rating scale for depression (HAMD) and traditional chinese medicine (TCM) symptoms scale were measured at week 1, 2, 4, and 6 after the treatment, and the effective rate was assessed according to HAMD and TCM symptoms scale to evaluate therapeutic effects. Adverse reactions were recorded. Result: Both of the effective rates of HAMD and TCM symptoms increased in two groups along with treatment time. After the first week of treatment, the total effective rate of HAMD in Guipi group (3.33%) was higher than that in the Fluoxetine group (0%) (P<0.01). However, there was no difference for total effective rate of HAMD between groups after 2, 4, or 6 weeks of treatment. The total effective rate of TCM symptoms in Guipi group were 10% and 53.33% after first and second week of treatment respectively, which were significantly higher (P<0.01) than that in the Fluoxetine group (3.33% and 16.67%). But no difference was found for effective rate of TCM symptoms between groups after 4 or 6 weeks of treatment. Furthermore, no adverse event was found in Guipi decoction group, while 1 case was dropped out after one week of treatment because of diarrhea in the Fluoxetine group, thus indicated the adverse reaction rate was 3.33% in the Fluoxetine group (P<0.01). Conclusion: Compared to Fluoxetine, Guipi decoction has similar effect on improving HAMD scores and TCM symptoms for depression patients with Heart-spleen deficiency, but it has fewer adverse reactions and faster onset of action. In conclusion, Guipi decoction is safe and effective for Heart-spleen deficiency depression.关键词：Guipi decoction;depression;Heart-spleen deficiency;Fluoxetine
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the efficacy and safety of sodium cantharidin injection plus radiotherapy compared with radiotherapy alone, in the treatment of esophageal cancer, we performed a Meta-analysis based on the literature. Method: Electronic databases of CBM, CNKI, Wangfang, VIP and PUBMED were searched, and the randomized controlled trials (RCTs) about sodium cantharidin injection plus radiotherapy for esophageal cancer were included. Quality of the included randomized clinical trials was assessed by Jadad scoring. And Meta-analyses were performed by using Stata software. Result: Three RCTs involving 253 patients were included in Meta-analyses. The quality of all studies was in low quality. The results of Meta-analyses showed that sodium cantharidin injection plus radiotherapy in the treatment of esophageal cancer, compared with control groups, could increase effective rate [RR=1.283,95%CI(1.076,1.530)] and clinical benefit rate [RR=1.112,95%CI(1.024,1.207)] and decrease leucocyte reduction rate[RR=0.239,95%CI(0.110,0.522)]. Conclusion: Sodium cantharidin injection plus radiotherapy can improve the therapeutic efficacy of radiotherapy for esophageal cancer and decrease bone marrow suppression, still, high quality RCTs were needed to provide further evidence to confirm its clinical efficacy and safety.关键词：sodium cantharidin;esophageal neoplasms;radiotherapy;randomized controlled trial;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: This study aimed to systematically review the efficacy and safety of Guanxin Danshen drop pill for coronary heart disease (CHD). Method: We search the literatures on randomized controlled trials (RCTs) using Guanxin Danshen drop pill for the treatment of coronary heart disease from public database such as Pubmed, Cochrane library, China Biology Medicine disc(CBM), CNKI, and Wanfang Data. RevMan5.2 was used in the analysis. Result: Fifty RCTs including 1 455 patients met the inclusion criteria. All the trials were not adequate in methodological quality. None of them was double-blinded trial. There was a significant funnel plot asymmetry, which means publication bias has been found. Compared with the control group, the treatment group showed significantly higher total effective rate at improving angina symptoms, and the total effectiveness rate at ameliorating the electrocardiogram effect, as well.As for the indexes of blood rheology, blood viscosity, plasma viscosity, hematocrit and fibrinogen in treatment group were superior to the contrast group(P<0.01), weighted mean difference were [-0.86,95%CI(-1.43,-0.28)],[-0.35,95%CI(-0.56,-0.15)],[-3.3,95%CI(-4.07,-2.53)],[-0.76,95%CI(-1.05,-0.48)]. There were no obvious side-effects found in the trials. [WTHZ]Conclusion:[WTBZ]The present Meta-analysis suggested that on the basis of conventional western medicine, Guanxin Danshen drop pill is helpful to improve symptoms, alleviate the ischemia of electrocardiogram and improve blood rheology indexes, however, high-quality evidences of evidence-based medicine(EBM) are needed due to publication bias and low quality of RCT.关键词：Guanxin Danshen drop pill;coronary heart disease;randomized controlled trials;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: The study aimed to predict the mechanism of effective components in anticoagulant herbs in molecular level through network pharmacology technology. Method: Virtual screening and network pharmacology were applied to study the interaction between natural molecules and the targets. Result: Herbs for promoting blood circulation and removing blood stasis had predictive anti-coagulative effects. Several potential effective components were screened out. Conclusion: Computational techniques can improve our understanding of the complex mechanisms of traditional medicine, and instruct the research in the future.关键词：anticoagulation;network pharmacology;promoting blood circulation and removing blood stasis;virtual screening
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发布时间：2024-01-04 - 摘要：According to the current research status of pesticide residues in Chinese herbal medicines, the best pesticide detection and removal method should be screened in order to reduce or avoid pesticide residue in Chinese herbal medicines. On the basis of recent literatures on Chinese pesticide residue, we summarized the type, detection method and the removal of pesticide residue in Chinese herbal medicines, and screened out the best methods for pesticide detection and removal. At present, the organochlorine pesticides were the main residue matters. The main detection methods of pesticide residue were gas-mass spectrometry (GC-MS) or gas chromatography (GC) testing, and pesticide residue removal relied mainly on supercritical fluid extraction method.关键词：pesticide residues;detection method;removal
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发布时间：2024-01-04 - 摘要：This article summarized the mechanism of ventricular remodeling after intervention of Chinese herbal compound in acute myocardial infarction based on the collection and analysis of the relavant literatures from different periodicals in recent years. Databases such as wangfang, CNKI and other abroad database were searched to consult the research papers which are about mechanisms of ventricular remodeling after intervention of Chinese herbal compound in acute myocardial infarction. The mechanisms mainly related to reduction of the load on the ventricular stimulation, improvement of ventricular wall resilience, inhibition of inflammation, regulation of neuroendocrine and anti-oxidative response, etc. Current research indicated that Chinese herbal compound can function in ventricular remodeling after intervention of Chinese herbal compound in acute myocardial infarction through various ways, which showed great advantages and prospects, however, in short of sufficient evidence-based clinical research. In addition, the molecular mechanisms should be further strengthened.关键词：Chinese herbal compound;acute myocardial infarction;ventricular remodeling
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发布时间：2024-01-04 - 摘要：Myocardial no-reflow phenomenon still have induced the incidence of major cardiovascular events after percutaneous coronary intervention in patients with acute myocardial infarction and can severe affect the prognosis, and its pathological proecsses includes vascular endothelial injury, inflammatiory reaction, oxygen free radical damage and platelet activation, etc. Although many drugs have been studied for intervention of specific pathological proecsses in no-reflow phenomenon, the effects were rarely observed because of its single target intervention. So it is urgent to resolve that how to improve blood flow perfusion of myocardium microcirculation after revascularization for acute myocardial infarction. Traditional Chinese medicines have been taken on significant effects on myocardial no-reflow phenomenon although few reports are showed. We deduced that insidious phlegm blocking collaterals was the major pathogenesis of myocardial no-reflow phenomenon after myocardial ischemia reperfusion by analysis of related literature under the guidance of the basic theory of traditional Chinese medicine (TCM), and insidious phlegm could closely relate to the hyperlipidemia and inflammation. The interaction between the hyperlipidemia and inflammation in pathophysiological processes demonstrated pathogenic characteristics of the phlegm inducing blood stasis and phlegm-stasis blocking collaterals in TCM, that is basic cause leading to poor effects and prognosis of PCI. Thus, it is an important method to improve no-reflow or slow blood flow after PCI that applying treatment of boosting qi for resolving phlegm and promoting blood circulation for removing obstructionin collaterals. This method could combine multiple effects in pathological proecsses of myocardial ischemia reperfusion and delay the endpoint events, so that improve the quality of life and reduce mortality at maximun degree. There is no doubt that the effective methods of myocardial no-reflow prevention and treatment are well widen in clinical medicine of TCM, which has the certain directive meaning in clinically.关键词：percutaneous coronary intervention;no-reflow;insidious phlegm;pathogenesis
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发布时间：2024-01-04
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