最新刊期
卷 20 ， 期 21 ， 2014
- 摘要：Objective: To determine dissolution of different extracts of Scutellariae Radix and investigate its oxidation resistance. Method: According to pre-treatment process to collect extracts of Scutellariae Radix containing different associated substances(The content of baicalin were 19.75%,62.83%,84.55% and 98.0%),HPLC was employed to determine dissolution of baicalin in each sample with mobile phase of methanol-water-phosphoric acid(47:53:0.2) and detection wavelength at 280 nm.Antioxidant capacity of different extracts of Scutellariae Radix was measured by DPPH,detection wavelength was set at 516 nm. Result: The higher baicalin content in different associated substances,the lower bioavailability;cumulative dissolution rates of these four extracts in 3 h were 38.24%,25.91%,23.49% and 22.98%.With increasing the content of baicalin in extracts,antioxidant capacity increased,there were no obvious changes in antioxidant capacity when the content of baicalin reached above 85%. Conclusion: Different quality and quantity of associated substances have a certain effect on dissolution and oxidation resistance of baicalin.关键词：Scutellariae Radix extracts;dissolution;DPPH radical scavenging method;antioxidant;associated substances
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发布时间：2024-01-04 - 摘要：Objective: To prepare bakuchiol-baicalin compound microemulsions and investigate its in vitro transdermal diffusion characteristics. Method: With ethyl oleate as oil phase,tween-80 as emulsifier and polyethylene glycol-400(PEG-400) as co-emulsifier,aqueous phase was gradually added into mixture containing drug to produce bakuchiol-baicalin compound microemulsions.Morphology,particle size distribution and stability were characterized.Transdermal characteristics of bakuchiol and baicalin in solution and microemulsions were studied by modified Franze diffusion cell. Result: Clear and transparent microemulsion were prepared,they were showed nearly spherical under transmission electron microscope,particle size was (17.30±0.17)nm with excellent stability.Cumulative permeation amounts of baicalin in solution and microemulsions were (208.80±5.26)μg·cm-2 and (232.38±15.07)μg·cm-2 in 24 h,there were no significant difference of their transdermal characteristics.Cumulative permeation amounts of bakuchiol in solution and microemulsions were (324.81±56.63)μg·cm-2 and (721.30±108.88)μg·cm-2 in 24 h,compared with steady-state infiltration rate and apparent permeability coefficient in solution,these two parameters 5.0 times significantly improved. Conclusion: Delivery system of microemulsions can significantly improve percutaneous absorption.This study provides experimental foundations for development of a new preparation of bakuchiol-baicalin compound skin delivery system.关键词：bakuchiol;baicalin;bakuchiol-baicalin compound microemulsions;percutaneous penetration characteristics;apparent permeability coefficient;steady-state infiltration rate
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction and purification procedures of Xiyue granules. Method: HPLC-ELSD was employed to determine the content of astragaloside IV with mobile phase of acetonitrile-water(35:65). HPLC was adopted to determine the content of gentiopicroside by taking methanol-water(27:73) as mobile phase,flow rate was 0.8 mL·min-1 and detection wavelength was 271 nm.Taking composite score of contents of astragaloside IV and gentiopicroside as index,effects of the amount of water,extracting time and times on extraction process was optimized by orthogonal design.Single factor tests were adopted to optimize purification procedure with dry extract rate and contents of astragaloside IV and gentiopicroside as indexes. Result: The best extraction process was as follows:extracted 3 times with 10 times the amount of water for 1.0 h each time;contents of astragaloside IV and gentiopicroside were 4.34,43.7 mg,respectively.Optimum purification process was ethanol precipitation which contained 50% alcohol;contents of astragaloside IV and gentiopicroside,dry extract rate were 21.30 mg,216.32 mg and 22.13%. Conclusion: Optimized extraction and purification processes are stable and reliable,they can provide references for industrial production of Xiyue granules.关键词：Xiyue granules;astragaloside IV;gentiopicroside;alcohol precipitation;Ultrafiltration
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发布时间：2024-01-04 - 摘要：Objective: To improve extraction efficiency of DNA in Asini Corii Colla and provide a reference for extracting DNA in other products containing high protein content. Method: DNA in Asini Corii Colla was extracted by SDS method and CTAB method,according to their own characteristics of Asini Corii Colla products,reaction time of proteinase K and protein elution step in SDS method were optimized. Result: Under the same conditions,average extraction capacity of DNA in Asini Corii Colla by SDS was higher than CTAB,whose numerical difference was 0.022 g·L-1.Optimized SDS method was as following:reaction time of proteinase K 1.5 h,eluted protein in Asini Corii Colla by phenol-chloroform-isoamyl alcohol for three times,purity of DNA in Asini Corii Colla increased 48.3% by comparing with the original process. Conclusion: Scavenging effects on protein in Asini Corii Colla by SDS method is higher than CTAB,this optimized SDS method is suitable for extraction of DNA in Asini Corii Colla,it provides conditions for biological detection of animal derived molecules from Asini Corii Colla.关键词：Asini Corii Colla;DNA extraction;proteinase K;phenol-chloroform-isoamyl alcohol
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Shihu Qingyan lozenges by Box-Behnken design-response surface methodology. Method: HPLC was employed to determine the content of dendrophnol with detection wavelength at 278 nm and mobile phase of acetonitrile(A)-0.5% formic acid(B) for gradient elution(0-20 min,27% A;20-30 min,27% -40% A;30-45 min,40% -65% A;45-50 min,65% -27% A).On the basis of single factor tests,a three-factor and three-level Box-Behnken experimental design was employed to investigate effects of extraction time,solid-liquid ratio and soaking time on composite score of extracting amount of dendrophnol and dry extract rate. Result: Optimum process conditions were as follows:soaked 35 min with 11 times the amount of water,extracted thrice for 1 h each time;extracting amount of dendrophnol was 0.189 6 mg·g-1 and dry extract rate was 41.27%. Conclusion: This optimized extraction technology is practical and rational by Box-Behnken design-response surface methodology and overall desirability,it provides a reference for application of Shihu Qingyan lozenges.关键词：Shihu Qingyan lozenges;Box-Behnken design;overall desirability;Platycodonis Radix;Dendrobium aurantiacum;dendrophnol
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining dissolution of lactucin in total sesquiterpenes from Cichorium glandulosum dropping pills. Method: Paddle method was adopted in which artificial gastric juice was taken as solvent with a rotating speed of 100 r·min-1.Dissolution of lactucin was determined by HPLC and dissolution curves were drawn,mobile phase is made up of methanol(A)-0.2% formic acid(B) for gradient elution(0-30 min,28% -72% A) and detection wavelength was set at 256 nm. Result: Lactucin was linear over the range of 0.015-0.075 g·L-1 with r of 0.999 and average recovery was 101.04% with RSD of 1.38%.Taking artificial gastric juice containing 0.2% sodium dodecyl sulfate as dissolution medium,dissolution of samples were all more than 80% in 30 min. Conclusion: This method is simple,accurate and reliable for dissolution determination of total sesquiterpenes from Cichorium glandulosum dropping pills.关键词：Cichorium glandulosum;total sesquiterpenes;dissolution;lactucin
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发布时间：2024-01-04 - 摘要：Objective: To prepare pH-dependent total alkaloids from Coptidis Rhizoma colon targeted pellets for treatment of ulcerative colitis and finish its in vitro and in vivo release performance evaluation. Method: Acrylic resin S100(eudragit S100) and eudragit L100-55 double-coated was adopted to prepare colon targeted pellets of total alkaloids from Coptidis Rhizoma.In vitro release and in vivo site-specific drug release of this preparation were evaluated with berberine hydrochloride as index. Result: In vitro release test showed that cumulative release rate of berberine hydrochloride was less than 0.1% in artificial gastric juice after 2 h and less than 10% in artificial intestinal fluid after 4 h,but cumulative release rate in artificial colon juice after 3 h was more than 90%.In vivo test showed that coated pellets could complete arrive to the cecum or colon in rats,and began to disintegrate and release. Conclusion: Colon targeted pellets of total alkaloids from Coptidis Rhizoma can achieve targeted release in the colon.关键词：colon targeted pellets;Coptidis Rhizoma;total alkaloids;in vitro release;berberine hydrochloride
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total polyphenols from seeds of Cleome gynandra and evaluate its antioxidant activities. Method: UV was adopted to determine the content of total polyphenols with detection wavelength at 760 nm.Taking the content of total polyphenols as index,selecting solid-liquid ratio,extracting temperature,solvent concentration,extracting time as factors,extraction conditions of total polyphenols were optimized by single factor tests and orthogonal test,its scavenge activity on DPPH·free radical was discussed. Result: The best extraction technology was as follows:extracted 40 min with 20 times the amount of 70% ethanol;the content of total polyphenols was 1.93 mg·g-1.Antioxidative activity test showed that DPPH·scavenging abilities of total polyphenols extract from seeds of C. gynandra and vitamin C had no difference when its concentration of 1.28 g·L-1,they were more than 96%. Conclusion: This optimized ultrasonic extraction technology is stable and feasible,total polyphenols have strong antioxidant activity,this article can provide experimental basis for development and utilization of seeds of C. gynandra.关键词：seeds of Cleome gynandra;total polyphenols;antioxidant activities;ascorbic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of effective fraction in Garidi powders and provide experimental evidence for pilot production at later stage. Method: The content of total alkaloids was determined by non-aqueous titration;UV was adopted to determine the content of total flavonoids with detection wavelength at 500 nm.Taking contents of total alkaloids and total flavonoids as indexes,orthogonal test was used to optimize extraction process with ethanol concentration,solid-liquid ratio,extracting time and times as factors,TLC and LC-MS were employed to confirm alkaloids types in extract. Result: Influencing factors acting on yield of total flavonoids was in the order of reflux time > solid-liquid ratio> ethanol concentration > extraction times,optimum extraction conditions of total flavonoids was:extracted 5 h with 22 times the amount of 70% ethanol.Factors acted yield of total alkaloids by ethanol concentration>reflux time>solid-liquid ratio>extraction times,optimum extraction conditions was as followings:extracted 4 h with 26 times the volume of 80% ethanol;mass fractions of total alkaloids and total flavonoids were 0.139% and 0.465%,respectively. Conclusion: According to actual conditions of production and cost,optimum extraction conditions of Garidi powders is as following:reflux extract 5 h with 22 times the volume of 70% ethanol.Main compositions of alkaloids in Papaver nudicaule are nudicauline,amurensinine,reframidine and amurensine.关键词：Garidi powders;Papaver nudicaule;Artemisia frigida;effective parts;ulcerative colitis
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction procedure of Guizhi Gancao Tang. Method: Contents of liquiritin,cinnamic acid,cinnamaldehyde and glycyrrhizic acid were determined by HPLC,mobile phase was consisted of acetonitrile(A)-water containing 0.1% phosphoric acid(B) for gradient elution(0-10 min,5% -20% A;10-15 min,20% -23% A;15-30 min,23% -32% A;30-40 min,32% -35% A;40-50 min,35% -40% A;50-60 min,40% -50% A), detection wavelength was at 254 nm.Orthogonal design was adopted to optimize extraction process with composite score of contents of liquiritin,cinnamic acid,cinnamaldehyde and glycyrrhizic acid as index,the amount of water,extracting time and times as factors. Result: Optimal extraction technology was:extracted thrice with 12 times the amount of water for 1.0 h each time;extracting amounts of liquiritin,cinnamic acid,cinnamaldehyde and glycyrrhizic acid were 5.93,11.73,12.25,12,34,172.86 mg·g-1. Conclusion: Syhthetic weighted mark method can reasonably reflect overall efficacy of Guizhi Gancao Tang,this article provides experimental basis for clinical application and development of this compound.关键词：comprehensive weighted scoring method;Guizhi Gancao Tang;liquiritin;cinnamic acid;cinnamaldehyde;glycyrrhizic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize vacuum-belt drying process parameters of Yangzheng Xiaoji capsules extract. Method: HPLC-ELSD was employed to determine the content of oleanolic acid with mobile phase of acetonitrile-methanol-0.5% acetic acid(20:70:10).Taking water content of dry extract and transport rate of oleanolic acid as indicators,orthogonal design was adopted to investigate effects of feeding speed,belt speed and heating system temperature on vacuum-belt drying process of Yangzheng Xiaoji capsules. Result: Optimal parameters of vacuum-belt drying process were as follows:belt speed of 6 cm·min-1,feeding speed of 12 mL·min-1,heating system temperature at 110 ℃,material temperature of 30 ℃,cooling zone temperature at 45 ℃,initial solid content in extract about 45%,vacuum degree Conclusion: This optimized technology is reasonable and feasible with low water content and good quality,which is suitable for large scale production of Yangzheng Xiaoji capsules.关键词：Yangzheng Xiaoji capsules;vacuum-belt drying;oleanolic acid;moisture content
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发布时间：2024-01-04 - 摘要：Objective: To optimize percolation parameters of Pheretima. Method: By using external hemolysis method,with solid content and protease activity as indicators,single factor tests were adopted to optimize percolation process. Result: The concentration of urokinase in 25-125 U·mL-1 showed a good linear relationship with soluble circle area,the best percolation processing parameters were:crushed Pheretima to a coarse powder,soaked 0.5 h with percolation rate of 2 mL·min-1,added 8 times the amount of 60% ethanol for percolation;percolate volume,solid content and protease activity were 220 mL,3.252 g,5 624.7 U·g-1. Conclusion: Fibrinogen plate method can be fast,easy and accurate to determine enzyme activity in Pheretima,these optimized process parameters was stable and feasible,it provides new choice for full utilization of active ingredients from Pheretima.关键词：Pheretima;enzyme activity;percolation process;fibrinogen plate method
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Arctii Fructus in Vitamin C Yinqiao Pian by dynamic optimization extraction method(DOEM). Method: HPLC was employed to determine the content of arctiin with detection wavelength at 280 nm and mobile phase of acetonitrile-water(20:80).Taking yield and content of arctiin,fingerprints similarity as indexes,feasibility of DOEM using in Arctii Fructus from Wei C Yinqiao tablets was evaluated,and compared with extraction process in 2010 edition of . Result: There was no difference in extract between dynamic optimization process and ordinary extraction process.Optimized DOEM was as following:reflux extracted twice with 8 times the amount of 60% ethanol for extracting time of 140 min and 60 min;it could save 58% of extraction time and 53% of power consumption. Conclusion: DOEM can be applied to optimize extraction process of Arctii Fructus in Vitamin C Yinqiao Pian,it can significantly reduce extraction time and production costs.关键词：dynamic optimization method;Arctii Fructus;arctiin;extraction time
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Pingchuan Futie plasters. Method: Taking composite score of extracting amounts of astragaloside,total polysaccharides,ephedrine hydrochloride and pseudoephedrine hydrochloride as index,based on single factor tests,effects of the amount of water,extraction time and times on extraction process was investigated by L9 (34) orthogonal test.HPLC-ELSD was adopted to determine the content of astragaloside with mobile phase consisting of acetonitrile-water(32:68).HPLC was adopted to determine contents of ephedrine hydrochloride and pseudoephedrine hydrochloride with mobile phase of acetonitrile-0.6% phosphoric acid(3:97) and detection wavelength at 208 nm.Phenol-sulfuric colorimetric method was used to determine the content of total polysaccharides. Result: A good linearity was obtained over concentration ranges of 1-17,0.2-2,0.1-1 μg for astragaloside,ephedrine hydrochloride and pseudoephedrine hydrochloride,respectively;while 3.27-7.63 mg·L-1 for total polysaccharides.Optimum process was adding 10 times amount of water for extracting three times,1.5 h each time;extracting amounts of astragaloside,total polysaccharides,ephedrine hydrochloride and pseudoephedrine hydrochloride were 0.204,0.900,1.063 g·g-1,respectively. Conclusion: This extraction process is simple and feasible with good repeatability,which can be effectively used to evaluate quality of Pingchuan Futie plasters.关键词：Pingchuan Futie plasters;astragaloside;ephedrine hydrochloride;total polysaccharides;Armeniacae Semen Amarum;Astragali Radix;Ephedrae Herba
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发布时间：2024-01-04 - 摘要：Objective: To develop a method of high performance liquid chromatography coupled with a photodiode array detector(HPLC-DAD)for the content determination of six active flavonoids (quercetin-3-O-sambubioside, rutin and quercetin-3-O-β-D-glucopyranside, kaempferol-3-O-β-D-glucopyranside, quercetin, kaempferol)in Oldenlandia diffusa. Method: The analyses were performed on a Diamonsil-C18 column (4.6 mm×250 mm, 5 μm) eluted with acetonitrile and 0.05% phosphoric acid in gradient mode. The flow rate was 1 mL·min-1, the detection wavelength was 254 nm, the column temperature was set at 30 ℃. Result: Six flavonoids have good linear relationship, precision, stability, and repeatability according to the requirements of the methodology determination. The recoveries were 99.40% -99.70%. Six flavonoids in O. diffusa from different origins were determined by HPLC-DAD. Conclusion: The method is simple,accurate,replicable,and can be used for the quality control of flavonoids in olden landia diffusa.关键词：Oldenlandia diffusa;HPLC-DAD;active flavonoids
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发布时间：2024-01-04 - 摘要：Objective: To set up a HPLC method for the determination of loganin in Zuogui Wan, Yougui Wan and their disassembled prescriptions. It can provide a quality control standard for Zuogui Wan, Yougui Wan and their disassembled prescriptions. Method: HPLC, a ZOREAX SB-C18 column served as the solid phase, the mobile phase was acetonitrile-water in gradient elution mode. The flow rate was 1.0 mL·min-1 and the sample volume was 20 μL each time, the detection wave length was 240 nm and the column temperature was 30 ℃. Result: The topic establishing method can better determine the content variation of loganin.Both before and after the peak position without impurity peak interference, and the peak was intact.The linear range of loganin was 0.289 0-7.225 0 μg(r=0.999 6).The average recoveries were 99.70%, with RSD of 1.5%.Stability, precision and the rate of sample recovery were all well.According to the results, the content of loganin in common prescription was the highest, while it was low in Zuogui Wan. Yougui Wan and kidney-Yin-nourishing drug has a similar level. Conclusion: The method is simple, accurate and reliable with good reproducibility.It can be used to provide the reference value and guiding significance for Zuogui Wan, Yougui Wan and their disassembled prescriptions' quality control.关键词：Zuogui Wan;Yougui Wan;loganin
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发布时间：2024-01-04 - 摘要：Objective: To study the isolation and determination of verbascoside from Callicarpa longissima. Method: Verbascoside was extracted by 95% ethanol, isolated and purified by column chromatography and PHPLC from the stem and branch of C. longissima. A HPLC method was set up to determine the content of verbascoside in C. longissimae, using ECOSIL ODS-EXTEND(4.6 mm×250 mm, 5 μm) column, and acetonitrile -0.2% phosphoric acid water solution as the mobil1-9e phase, the UV detection wavelength was at 334 nm, with the flow rate of 1.0 mL·min-1 at room temperature. Result: A good linear was obtained in the range of 0.051 9-0.829 6 μg and the average recovery was 99.13% (RSD 1.80%). Conclusion: Verbascoside was isolated from C. longissima for the first time.The method can provide useful references to quality control of C. longissima.关键词：Callicarpa longissima;verbascoside;isolation;determination
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发布时间：2024-01-04 - 摘要：Objective: To study the rapid detection of phenolphthalein content adulterated to Chinese pattern diet drugs by Raman Spectroscopy. Method: In this paper, 4 portions of Chinese pattern diet drugs were extracted by 5 mL of methanol, sonicated for 10 min, then filtered and tested directly by raman spectroscopy. Result: Calibration curve was defined and analysis was performed on samples with different contents of Phenolphthalein. Results were in accordance with real amount of adulteration. The concentration of phenolphthalein and the intensity of its raman signal was in good linear at the concentration above 0.1% (r=0.998 1, RSD 3.7%). Conclusion: Samples adulterated with phenolphthalein, pretreated by a suitable method could be fast screened by raman spectroscopy. This method is fast, simple and low-cost with the detection limit at 1% for phenolphthalein. Either qualitative or quantitative results can be obtained simultaneously.关键词：Raman spectroscopy;Chinese pattern diet drugs;phenolphthalein;adulteration
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发布时间：2024-01-04 - 摘要：Objective: To establish method of analyze volatile constituents from Artemisiae Argyi Folium by headspace solid-phase microextraction with gas chromatography-mass spectrometry (HS-SPME-GC-MS) technique, and analysis different factors' results on extraction effect. Method: After single factor test, the sum of the peak area of the volatile constituents was as index to optimum extraction conditions, while consider pair wise interactions. Result: The optimum HS-SPME parameters were selected as:1.0 g sample, 80 ℃ extracted for 40 minutes. The volatiles were extracted using a 100 μm polydimethylsiloxane (PDMS) fiber, the desorption time was 3 minutes at 250 ℃ and adjustable depth gauge was #2.Analyzing the sample with the optimum methods, a total of 84 chromatographic peaks were resolved, and 66 constituents were identified, which accounted for 94.46% of the total relative contents. Conclusion: Extraction temperature is the main factor, followed by the interaction between the extraction temperature and sample quality, then the extraction time, and finally the quality of the sample. Other factors have a significant impact on the extraction effect in addition to the sample quality (P<0.05). It provides the basis for development and utilization of the Artemisiae Argyi Folium.关键词：Artemisiae Argyi Folium;headspace solid-phase microextraction;volatile constituents;Orthogonal Design
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发布时间：2024-01-04 - 摘要：Objective: A rapid and sensitive method for HPLC was established to simultaneouly determine chlorogenic acid,forsythin, baicalin,wogonin and apigenin. Method: Chromatographic separation was carried out on Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm) under gradient elution(0-0.01 min,A phase is 25%;0.01-10 min,A phase is 25% -53%;10-16 min,A phase is 53% -78%) with methanol (A phase)-water/0.2% phosphate(B phase) as mobile phase at a flow rate of 1.00 mL·min-1 to elute at 25 ℃. The injection volume was 20 μL and the detection was adopted UV detector at 278 nm. Result: These five components were seperated basically within 25 minutes with good linearity.The regression equations were Y=2 350.4X-137.27,r=0.999 6(for chlorogenic acid),Y=1 161.7X+18.474,r=0.999 6(for forsythin),Y=3 204.2X-423.45,r=0.998 8(for baicalin), Y=4 792.7X-93.15,r=0.998 5(for wogonin),Y=1 434.1X-8.413 2,r=0.999 3(for apigenin).The average recoveries were:chlorogenic acid 99.47% (RSD 0.8%), forsythin 98.26% (RSD 0.9%),baicalin 100.4% (RSD 1.9%),wogonin 99.29% (RSD 1.1%),apigenin 98.79% (RSD 1.1%). Conclusion: The HPLC method has good accuracy,reliability, and repeatbility.It is suitable for compound Honeysuckle granules and traditonal Chinese medicines containing the same ingredients to improve their quality control.
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发布时间：2024-01-04 - 摘要：Objective: To improve the quality standard of Lancong Cha. Method: Canarii Fructus,Perillae Folium and Zingiberis Rhizoma Recens in the recipe were identified by TLC. The contents of rosmarinci acid and gallic acid were determined by HPLC. Result: The TLC spots were clearly and separated well, and the negative control did not impose any disturbance. Rosmarinci acid had good linearity in a range of 0.098-4.912 μg(r=0.999 9). The average recovery was 100.24%, and RSD was 2.9%. Gallic acid had good linearity in a range of 0.100 2-1.503 0μg (r=0.999 97). The average recovery was 99.02%, and RSD was 1.8%. Conclusion: The method was specific, sensitive and repeatable, can be used to control the quality of Lancong Tea better.关键词：Lancong Cha;TLC;HPLC;rosmarinci acid;gallic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish evaluation method on antioxidant activity of Pyracantha Fortuneana extract by DPPH. Method: The effect of DPPH and ethanol concentration,light,temperature and pH on relative absorbance of DPPH ethanol solution,as well as the effect of reaction time and temperature,pH,light and ethanol concentration diluting Pyracantha Fortuneana extract were both investigated thoroughly. Result: DPPH ethanol solution must be prepared just before using and can be saved in the refrigerator (2 ℃) for no more than 4 hours without light,whose concentration should be controlled between 0.02 and 0.2 mmol·L-1. The absorbance of sample solution could be measured at 517nm after being diluted with anhydrous ethanol and reacted for 30 min under 25 ℃ excluding light,along with the optimal pH is from 3 to 8.We had better controlled the sample dilution times and ensured that DPPH clearance ratio is between 17.70% and 53.43%. Conclusion: This evaluation method is reliable and stable,as well as good repeatability.关键词：DPPH evaluation method;Pyracantha Fortuneana extract;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Scutellaria barbata. Method: The compounds were isolated and purified by chromatographies on silica gel column and Sephadex LH-20 column together with recrystallization method, and their structures were elucidated on the basis of physical and spectral analyses. Result: Eight compounds were isolated and identified as p-hydroxyacetophenone (1),p-hydroxybenzaldehyde(2), 2-hydroxy-3-methylanthraq-uinone (3), vanillin (4), 5-hydroxy-7, 3', 4', 5'-tetramethoxyflavone (5), dihydrooroxylin A (6), 5, 7, 3', 4', 5'-pentamethoxyflavone (7), hyperoside (8). Conclusion: Compounds 4,7 and 8 were obtained from Scutellaria for the first time. Compound 6 was obtained from the title plant for the first time.关键词：Scutellaria barbata;chemical constituents;structure identification
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发布时间：2024-01-04 - 摘要：Objective: To analysis on the volatile components of Mentha haplocalyx from Baotianman mountan, Banping town and Kaifeng in Henan Province by GC-MS. Method: The volatiles were analyzed by head-space solid micro-extraction, coupled with GC/MS and Kovats. Result: The 21 compounds were identified from the M. haplocalyx collected from Baotianman, 29 from the M. haplocalyx collected from Banshanping town and 33 from the M. haplocalyx collected from Kaifeng, 81.9%, 96.61% and 67.79% of the total volatiles, respectively. The major volatile components of M. haplocalyx collected from Baotianman were caryophyllene oxide (46.59%), spathulenol (6.79%), caryophyllene (4.57%) and D-(+)-carvone (3.89%). That collected from Banshanping town were isomenthol (61.1%), caryophyllene (10.22%), and menthone (5.64%).That from Kaifeng were pulegone (35.97%), β-cubebene (5.78%), isomenthone (5.10%), piperitone epoxide (4.17%) and menthyl acetate (4.03%). Conclusion: There was a difference in the composition and the rate of composition in the volatile components of M. haplocalyx from three different origin in Henan province.关键词：Mentha haplocalyx;volatile constituents;SPME;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for simultaneous determination of four catechins in Laportea bulbifera. Method: The chromatography separation was performed on a Diamonsil C18 (4.6 mm×250 mm, 5 μm) column with a gradient solvent system of acetonitrile-0.1% phosphoric acid as the mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm and the column temperature was kept at 35 ℃. Result: The linear ranges of (-)-gallocatechin, (±)-epigallocatechin, (+)-catechin, (-)-epicatechin were 0.050 50-1.010 μg (r=0.999 9),0.050 05-1.001 μg (r=0.999 9),0.025 75-0.515 0 μg (r=0.999 9) and 0.016 65-0.333 0 μg (r= 0.999 9), respectively. The average recoveries of four components were 97.39%, 98.45%, 99.17% and 97.87%, respectively. Conclusion: The method is simple, accurate and repeatable, and could be used to control the quality of L. bulbifera.关键词：Laportea bulbifera;HPLC;(-)-gallocatechin;(±)-epigallocatechin;(+)-catechin;(-)-epicatechin
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发布时间：2024-01-04 - 摘要：Objective: To establish a suitable inter-simple sequence repeat (ISSR)-polymerase chain reaction (PCR) system for analysis of genetic differences in Nerviliae fordii. Method: The ISSP-PCR was optimized using orthogonal design of five factors (dNTP, template DNA, primer, Mg2+,Taq DNA polymerase) at four levels. Single factor analysis was conducted using Duncan's new multiple range method. Result: An optimum ISSR-PCR reaction system established for N. fordii was as follows:20 μL ISSR-PCR system contained 60 ng template DNA, 2.0 μL of 10×PCR buffer, 1.0 U Taq polymerase, 225 μmol·L-1 dNTP mix, 2.5 mmol·L-1 of Mg2+, 0.4 μmol·L-1 of primers. Sixteen ISSR primers with stable amplification and abundant polymorphism were selected from 100 ISSR primers. Conclusion: The optimized and established ISSR reaction system is stable and credible according to the testing results of 24 samples of N. fordii, which provides methodology basis for the genetic analysis of N. fordii.关键词：Nerviliae fordii;inter-simple sequence repeat;optimization of reaction system;primers screening
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from Physalis Calyx Seu Fructus. Method: The chemical constituents were isolated and purified by silica gel, ODS column chromatography, Sephadex LH-20 and semi-preparing HPLC. Their structures were identified on the basis of ESI-MS, UV and NMR spectra. Result: Eight compounds were isolated and elucidated as (+)-syringaresinol-O-β-D-di-glucopyranoside (1), (+)-pinoresinol-O-β-D-di-glucopyranoside (2), (+)-medioresinol-O-β-D-di-glucopyranoside (3), apigenin-7-O-β-D-glucoside (4), chrysoeriol-7-O-β-D-glucoside (5), diosmetin-7-O-β-D-glucoside (6), scopoletin-7-O-β-D-glucoside (7) and syringalide B (8). Conclusion: Compounds 1-8 were firstly obtained from this plant.关键词：Physalis Calyx Seu Fructus;chemical constituents;structural identification
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发布时间：2024-01-04 - 摘要：Objective: The chemical constituents of extract of petroleum ether soluble part from leaves of Sambucus chinensis were investigated. Method: After being extracted with ethanol percolation, the chemical constituents were isolated and purified by column chromatography and their structures were identified by spectral analysis. Result: Eight compounds were obtained from leaves of S. chinensis and identified as ursolic acid (1), oleanic acid (2), β-sitosterol (3), daucosterol (4), squalene (5), phytol (6), n-pentacosanol (7), 4-hydroxyphenethyl heptadecanoate(8). Conclusion: Compounds 5-8 were isolated from genus Sambucu for the first time.关键词：Sambucus chinensis;petroleum-ether soluble part;chemical constituents;isolated and purified
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Blumea balsamifera. Method: The compounds were isolated by various chromatographic techniques including Silica gel, Sephadex LH-20, preparative HPLC, and their structures were identified by physicochemical and spectral data. Result: Eight compounds were isolated from B. balsamifera, and their structures were identified as liquiritigenin (1), β-sitosterol (2),kumatakenin (3), glycyrol (4), isoglycyrol (5), focosyl caffeate (6), inuchinenolide B (7), neogaillardin (8). Conclusion: Compounds 1, 3-8 were isolated from B. balsamifera for the first time.关键词：Huangyao’ in Yi nationality's medicine;Blumea balsamifera;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To investigate the components and antitumor activity against human tumor cell lines of fatty oil of Annona squamosa seeds. Method: The samples were derivated by methyl esterification, the components were identified by gas chromatography (GC) analysis and comparison of references, and the relative amount of these components were determined by peak area normalization method. In vitro anti-proliferative activity of the fatty oil against human liver cancer (SMMC-7721 and HepG2), human uterine cervix cancer (Hela), human lung cancer (A549) and human breast cancer (MCF-7) cell lines were tested by MTT assay. Result: The major chemical constituents of the fatty oil were fatty acid (80.9%), and the content of unsaturated fatty acid were 51.1%. The IC50 of the fatty oil against the 5 human cancer cell lines tested above was 10.4, 0.57, 30.3, 44.3, 6.7 mg·L-1 respectively. Conclusion: The main components of the fatty oil of A. squamosa seeds were methyl palmitate, methyl stearate, methyl oleate and methyl linoleicacid. They showed high selective inhibitation towards the proliferation of HepG2 cell line.关键词：Annona squamosa seeds;fatty oil;gas chromatography;human tumor cells
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents from the ethyl acetate and buthanol fractions of ethanol extract of the leaves of Polygonati Odorati Rhizoma. Method: The compounds were isolated by ODS chromatography and preparative HPLC, and their structures were elucidated on the basis of chemical and spectroscopic methods including MS, 1D and 2D NMR spectral techniques. Result: Ten compounds were isolated from the ethyl acetate and buthanol fractions of ethanol extract of the leaves of Polygonati Odorati Rhizoma, and they were identified as vanillic acid(1), trans-p-hydroxycinnamic acid(2), cis-3-hexenyl-β-D-glucopyranoside(3), N-trans-p-hydroxyphenethyl coumaramine(4), N-trans-p-hydroxyphenethyl ferolamine(5), apigenin(6), chrysoeriol(7), adenosine(8), saponarin(9), isovitexin(10). Conclusion: All compounds were obtained from the leaves of this plant for the first time, and compounds 1-4, 6, 8 and 10 were firstly isolated from genus Polygonatum.关键词：Liliaceae;leaves of Polygonati Odorati Rhizoma;chemical constituent
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发布时间：2024-01-04 - 摘要：Objective: An sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the determination of oxyresveratrol in rat bile. Method: The analyte and the internal standard (carbamazepine) were extracted from bile samples using simple liquid-liquid extraction technique with ethyl acetate,seperated on a ZORBAX SB-C18 column (2.1 mm×50 mm,1.8 μm) with mobile phase of acetonitrile and water containing 0.2% formic acid.Detected by triple quadruple mass spectrometry with an positive electrosprayionization interface, and quantified using multiple reaction monitoring (MRM) mode.A guard column was used and the flow rate was 0.4 mL·min-1. Result: It showed the highest biliary excretion at 0.5-1 h after administration.The biliary excretion rate of oxyresveratrol was 0.737% within 12 hours. Conclusion: This analytical method is rapid (3 min for each sample), selective, precise, accurate, and reliable for in vivo analysis of oxyresveratrol.关键词：oxyresveratrol;liquid chromatography-tandem mass spectrometry;bile;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprint for comparative studying on chemical substances difference between stems and leaves of Uncariae Ramulus Cum Uncis,in order to lay scientific basis for follow-up studies of Uncariae Ramulus Cum Uncis leaves. Method: Similarity of HPLC fingerprint was calculated by 2004A version of "traditional medicine fingerprint similarity software evaluation system" for 10 batches of stems and 10 batches of leaves in Uncariae Ramulus Cum Uncis.Taking rhynchophylline and isorhynchophylline as index components,HPLC analysis was performed on a Kromasil column(4.6 mm×250 mm,5 μm) with gradient eluting system consisted of methanol(A)-0.02% ammonia(B)(0-45 min,30% -60% A;45-75 min,60% -64% A;75-90 min,64% -70% A;90-95 min,70% -70% A),flow rate was 1.0 mL·min-1,column temperature was maintained at 30 ℃ and detection wavelength was set at 245 nm,injection volume of 10 μL. Result: Sixteen common peaks were obtained in 10 batches of Uncariae Ramulus Cum Uncis stems with similarity higher than 0.87 in each batches of samples;sixteen common peaks were obtained in 10 batches of Uncariae Ramulus Cum Uncis leaves with similarity higher than 0.80 in each batches of samples. Conclusion: The content of isorhynchophylline in leaves is higher than that in leaves,but the content of rhynchophylline have little difference.This established fingerprint conditions can be used as initial comparative studies on main chemical components of Uncariae Ramulus Cum Uncis stems and leaves,it provide a reference for medicinal research of Uncariae Ramulus Cum Uncis leaves.关键词：Uncariae Ramulus Cum Uncis stems and leaves;fingerprint;similarity evaluation;rhynchophylline;isorhynchophylline
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发布时间：2024-01-04 - 摘要：Objective: To compare monoester-alkaloids content of different batches of Xiaojin Wan from different manufacturers and provide a reference for clinical drug safety of this preparation. Method: Determination of monoester-alkaloids from Aconiti Kusnezoffii Radix Cocta in Xiaojin Wan was established,including benzoylmesaconine,benzoylaconine and benzoylhypacoitine;mobile phase was consisted of acetonitrile-20 mmol·L-1 sodium dihydrogen phosphate(adjusted pH to 6.5 with triethylamine)(47:53),detection wavelength was set at 235 nm.The content of these three components in 8 batches of Xiaojin Wan from four manufacturers was compared. Result: Linear ranges of these three components were 4-60 mg·L-1,recoveries were 98.42%,101.79% and 102.13%,respectively.Total content of these three components was up to 214.718 μg·g-1with the lowest value of 18.452 μg·g-1. Conclusion: There is a big difference among 3 kinds of monoester-alkaloids from different manufacturers and different batches of the same manufacturer,which may be related to quality of raw materials,preparation process,production equipment and other factors.关键词：Aconiti Kusnezoffii Radix Cocta;benzoylmesaconine;benzoylaconine;benzoylhypacoitine;Xiaojin Wan
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Xiaoer Fuxie Waifu powder on the urine of senna-induced diarrheal rats, identify the biomarkers, and explore its therapeutic mechanism. Method: Rat urine was collected and analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). The acquired data were analyzed using principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The concentration changes of endogenous metabolic biomarkers in the urine were studied. Result: Metabolite profiles of the urine samples among groups had been obviously classified. And samples showed clustering character in each group. Six metabolic biomarkers were tentatively identified as urobenzoic acid, citric acid, α-oxoglutarate, 2-aminobenzoicacid,L-citrulline and N-acetyl-5-HTA, which could be obviously affected by Xiaoer Fuxie Waifu powder. Conclusion: The administration of Xiaoer Fuxie Waifu powder could affect endogenous metabolites of urine, which may be its therapeutic mechanism.关键词：ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry;Xiaoer Fuxie Waifu powder;metabonomics
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发布时间：2024-01-04 - 摘要：Objective: To establish a quantitative analysis method for pharmacokinetics and bioavailability of breviscapine phospholipid complex self-microemulsions in Beagle dogs. Method: Beagle dogs were administered with breviscapine phospholipid complex self-microemulsions and breviscapine tablets at single dose,respectively.HPLC was adopted to determine the concentration of scutellarin in plasma with mobile phase of methanol-0.2% phosphoric acid solution(45:55) and detection wavelength at 335 nm.Pharmacokinetics parameters and bioavailability was calculated by DAS2.1.1 software program. Result: Plasma concentration-time profiles of scutellarin in these two preparations were fitted to double-compartment model,Tmaxof breviscapine phospholipid complex self-microemulsions and breviscapine tablets were 190 min and 160 min,Cmax were 78.98 mg·L-1 and 33.63 mg·L-1,AUC0-twere 18 674.619 mg·L-1·min and 9 132.475 mg·L-1·min,respectively.Relative bioavailability of self-microemulsions relative to tablets was 204.49%. Conclusion: Breviscapine phospholipid complex self-microemulsions can significantly improve bioavailability of scutellarin in Beagle dogs,which provides a new direction for development of oral preparations of breviscapine.关键词：breviscapine;phospholipid complex;self-microemulsions;pharmacokinetics;scutellarin;bioavailability;protocatechualdehyde
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发布时间：2024-01-04 - 摘要：Objective: By establishing UPLC method for determining binding rate between salvianolic acid B,danshensu and rat,bovine serum albumin(BSA),human plasma proteins,to analysis difference of plasma protein binding rate of these two components. Method: UPLC method was employed to determine contents of salvianolic acid B and danshensu with mobile phase of acetonitrile(A)-1% formic acid solution(B) for gradient elution(0-2 min,18% A;2-3 min,18% -30% A;3-6 min,30% A) and detection wavelength at 280 nm.Equilibrium dialysis method was used to investigate binding rates of salvianolic acid B and danshensu with different plasma proteins. Result: Linear ranges of salvianolic acid B and danshensu in 3 kinds of plasma were 2-200 mg·L-1,and in dialysis filtrates were 0.5-20 mg·L-1.Mean protein binding rates of salvianolic acid B in rat,human plasma and BSA were 90.60%,92.25%,87.55%,respectively;these of danshensu were 35.44%,37.71%,33.49%,respectively. Conclusion: Binding rates of different concentrations of salvianolic acid B and danshensu in the same plasma have no significant difference,but binding rates of salvianolic acid B with 3 kinds of plasma protein are higher than danshensu.关键词：salvianolic acid B;danshensu;plasma protein binding rate;equilibrium dialysis;UPLC
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发布时间：2024-01-04 - 摘要：Objective: To observe the long-term application of diazepam on drug tolerance in mice and different sedative medicine for its inhibitory effect,and discussed its mechanism. Method: Mice were divided into blank control group,model control group, Pinelliae Rhizoma-Husked Sorghum group(3.25 g·kg-1·d-1),Magnetitum-Cinnabaris group(0.33 g·kg-1·d-1),Coptidis Rhizoma-Asini Corii Colla group(2.73 g·kg-1·d-1), Coptidis Rhizoma-Cinnamomi Cortex group(2.15 g·kg-1·d-1) and Gardeniae Fructus-Sojae Semen Praeparatum group(1.69 g·kg-1·d-1),orally once a day for 60 days.During the 60 days,except the blank control group,each group mice were injected with diazepam in 30 minutes after taking drugs(25 mg·kg-1·3 d-1).Continuously monitor the mice sleep latency,determination of autonomic activity frequency and time,using fluorescence quantitative PCR determination of whole brain gamma-aminobutyric acid receptors(GABAA-R) mRNA in mice, glutamic acid decarboxy lase 65(GAD65) mRNA expression. Result: During the medication,sleep latency period of diazepam group extended gradually,Coptidis Rhizoma-Asini Corii Colla group and Coptidis Rhizoma-Cinnamomi Cortex group changed little;Medication after 2 months, compared with diazepam group,sleep latency period of Coptidis Rhizoma-Asini Corii Colla group and Coptidis Rhizoma-Cinnamomi Cortex group was obviously shortened(P<0.01), and mice autonomic activity times and activity time of Coptidis Rhizoma-Asini Corii Colla group,Coptidis Rhizoma-Cinnamomi Cortex group and Pinelliae Rhizoma-Husked Sorghum group significantly reduced (P<0.05,P<0.01),and Coptidis Rhizoma-Asinicorii Colla group,Coptidis Rhizoma-Cinnamomi Cortex group,Pinelliae Rhizoma-Husked Sorghum group, magnetitum-cinnabaris group and gardeniaefructus-sojaesemenpraeparatum group mice brain GABAA-R mRNA and GAD65 mRNA expression significantly higher (P<0.05,P<0.01). Conclusion: Coptidis Rhizoma-Asini Corii Colla,Coptidis Rhizoma-Cinnamomi Cortex and Pinelliae Rhizoma-Husked Sorghum can alleviate body tolerance in mice because of the long-term application of diazepam alone,the mechanism may be related to increase GABAA-R and GAD65 in the brain.关键词：diazepam;sedative Chinese medicine pairs;tolerance;gamma-aminobutyric acid
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effects and mechanisms of modified total alkaloids patch from Strychnou nux-vomica(MTAP) on adjuvant arthritis rats. Method: Sixty rats were randomly divided into adjuvant arthritis(AA) model group, normal group, Diclofenac Diethylamine Emulgel group (24 mg·kg-1), and MTAP groups (12,24,48 mg·kg-1). Complete Freund's adjuvant (FCA) was used to induce AA in rats. MTAP groups were treated with patch on abdominal hair removal area, the model group and the normal group were given blank matrix patch every other day for continuous 30 days. Paw swelling of AA rats was measured with volume meter. Meanwhile, splenic lymphocyte proliferation response induced by concanavalin A(Con A) or lipopolysaccharide(LPS) was examined with MTT assay. interleukin-1β(IL-1β), tumor necrosis factor α(TNF-α) production of peritoneal macrophage (PMΦ) were estimated by enzyme linked immune sorbent assay (ELISA). HE staining was used to examine the pathological changes in joint synovium of the rats. Result: Compared with normal group, more inflammatory cells infiltrated joint synovial tissues with congestion and edema in AA model group;T and B cell proliferation was significantly increased (P<0.05), the contents of IL-1β and TNF-α from PMΦ were also increased significantly (P<0.05). Compared with model group, AA rats with MTAP significantly attenuated primary and secondary hind paw swelling(P<0.05). Meanwhile, it remarkably regulated the splenic T and B cell proliferation response, down-regulated TNF-α production(P<0.05, P<0.01), improved the pathological impairment of joints. Conclusion: MTAP can ameliorate the symptom of adjuvant arthritis in rats. Its active mechanism may be related to modulation of the immune function of abnormal cells and the regulation of cytokines.关键词：modified total alkaloids patch from Strychnou nux-vomica;adjuvant arthritis;inflammation;immunomodulation
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of ethanol componentons from Aconitum vilmorinianum(AVEC) on inflammatory cytokines in lipopolysaccharide(LPS)-stimulated macrophage model. Method: The in vitro model of inflammation model based on the growth of well RAW 264.7 cells was established by treating with LPS (10 μg·L-1) for 24 h. The activities of macrophages were detected by MTT assay. The production of nitric oxide (NO) was assayed by Griess reagent. ELISA were used to assay the production of inflammatory mediators,such as tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in cell supernatant. Result: The cell viability was not significantly affected by up to 400 mg·L-1 of AVEC. Compared with the control group, LPS could induce RAW 264.7 cells to secrete inflammatory mediators like TNF-α, IL-6 and NO (P<0.01). Compared with the model group,100-400 mg·L-1 of AVEC in LPS-stimulated RAW 264.7 cells greatly inhibited the production of inflammatory mediators,such as TNF-α and IL-6 in a good dose dependent manner (P<0.05, P<0.01). Conclusion: AVCE can inhibit LPS-induced inflammatory response in RAW 264.7 cells, and its anti-inflammatory effect may be related to reducing the inflammatory cytokines like TNF-α, IL-6 and NO.关键词：Aconitum vilmorinianum;lipopolysaccharide;tumor necrosis factor α;interleukin 6
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发布时间：2024-01-04 - 摘要：Objective: Investigate the regulation of Danshen injection on intrauterine infection/inflammation in premature rats is periventricular leukomalacia brain pathology and synaptophysin (synapsin, SYP), nerve growth associated protein (growth-protein-43, GAP-43). Method: Using pregnant rat by intraperitoneal injection of LPS preparation intrauterine infection/inflammation in premature rats periventricular leukomalacia animal models, intraperitoneal injection with saline injection as normal control group, select the model group and the control respectively after birth group of pregnant rats uterus, placenta and brain tissue rats HE staining, after the success of the model, the model 40 preterm rats divided into Danshen high dose group A (13.5 g·kg-1), Danshen low dose group B (6.75 g·kg-1), ganglion glucoside grease sodium group C(6 mg·kg-1), model control group D (9 mL·kg-1) for each 10, normol control group E (normal saline 9 mL·kg-1) full term rats 10, each group in the eighth days after birth to intraperitoneal injection of intervention under 14 days, after 21 days of feeding the same conditions, neurological behavior detection, decapitated, SYP GAP-43 expression, detection HE staining, immunohistochemistry. days, after 21 days of feeding the same conditions, neurological behavior detection, decapitated, SYP GAP-43 expression, detection HE staining, immunohistochemistry. Result: Drug intervention group SYP, GAP-43 expression in the control group compared with the model group was significantly increased (P<0.01), with the control group was significantly lower (P<0.01). The model group that compared with normal control group expression reduced significantly (P<0.01).Drug intervention group Danshen injection high and low dose group, ganglion glucoside grease sodium group SYP, GAP-43 expression, no statistically significant difference in the three group. Conclusion: Danshen injection of intrauterine infection/inflammation-induced mechanism of preterm rats periventricular leukomalacia, and may raise SYP GAP-43 expression related.关键词：Danshen injection;periventricular leukomalacia;synapsin;growth-protein -43
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Wuji Wan decoction,compound and recipe granules on analgesic, anti-diarrheal and antagonism acute gastric ulcer functions. Method: The animals were divided into control group, positive control group, high,middle,low dose groups of Wuji Wan decoction,compound and recipe granules(mice:4.0,2.0,1.0 g·kg-1;rats:2.8,1.4,0.7 g·kg-1), 3-8 days after administration, analgesic, anti-diarrheal and acute gastric ulcer were observed with acetic acid writhing test, the diarrheal model was induced by senna and the model of acute gastric ulcer was induced by ethanol. Result: Compared with the control group, high, middle and low dose(4.0,2.0,1.0 g·kg-1) of Wuji Wan decoction, compound and recipe granules reduced the writhing times, decreased frequency of diarrhe, obviously reduced gastric mucosal injury in rats. Conclusion: Wuji Wan decoction, compound and recipe granules can decoction,compound and recipe granules show obvious analgesic, antidiarrheal and antagonism, acute gastric ulcer effects.关键词：Wuji pills;different dosage form;analgesic;anti-dianrrheal;anti-gastric ulcer
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Gegen Tongmai Yin on the endoplasmic reticulum stress related C/EBP-homologousprotein (CHOP) expression induced by homocysteine (HCY), in order to discuss anti-atherosclerosis (AS) molecular mechanism. Method: Thirty-six rabbits were randomly divided into 6 groups, the normal control group (the normal feed), the high-fat group (the normal feed+1% cholesterol+0.02% methionine), the rosuvastatin group (1 mg·kg-1+high-fat feed), the Gegen Tongmai Yin low, middle and high dose group (5,7.5,10 g·kg-1+high-fat feed).The rabbit AS model was induced by feeding the high-fat diet for 9 weeks. The superior segment of thoracic aorta was taken for the pathological observation, the CHOP expression was detected by situ hybridization and Western-blotting hybridization. Result: Pathological observation showed that the typical lipid plaque formed under the tunica intima in high-fat group, the pathological changes in the Rosuvastatin group were weaker than high-fat group, with small lipid plaque formed. The lipid plaque only formed in local areas in the Gegen Tongmai Yin low-dose group, the tunica intima structural disordered in the middle-dose group, only a small quantity cellular proliferation of smooth muscle in the high-dose group. Tissue in situ hybridization detection showed that CHOP gene expressed in the cytoplasm of tunica intima and tunica media in all groups. Small brownish yellow granules appeared in the endothelial cells and smooth muscle cells in normal control group. The brownish yellow granules were strongly expressed in the smooth muscle cells and foam cells in the high-fat group. The positive signal decreased expression in the smooth muscle cells in Gegen Tongmai Yin groups compared with the high-fat group. Western-blotting hybridization showed that CHOP protein were all expressed in 6 groups, CHOP protein expression increased in high-fat group compared with normal group. The difference was statistically significant(P<0.01);CHOP protein expression decreased in Gegen Tongmai Yin low, middle and high dose groups, the difference was significant(P<0.01). Conclusion: The Gegen Tongmai Yin's anti-AS mechanism may be correlated with the CHOP expression. The Gegen Tongmai Yin can improve the injury of blood vessel induced by HCY and depress the CHOP expression in the rabbit's thoracic aorta.关键词：Gegen Tongmai Yin;homocysteine;atherosclerosis;C/EBP-homologousprotein;thoracic aorta
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Huoxue capsule on atherosclerotic plaque in rabbits and explore the possible mechanism of stabilizing atherosclerotic plaque. Method: Fifty-three healthy male rabbits were randomly divided into two groups. The rabbits in blank group were fed with common forage. The rabbits in experimental group were fed with high lipid forage. After 10 weeks, rabbits in experimental group were randomly chose to be dissected and atherosclerotic plaque was found and high density lipoprotein-cholesterol(HDL-C) level in the blood was examined to verify whether the model is completed. Then all rabbits in model group were randomly divided into 4 groups:including the model group, the positive control group, and the groups of high and low dose Huoxue capsule, which were fed high lipid forage and blank group fed common forage continuously. Twenty weeks later,blood simples were collected to determine the serum total cholesterol(TC),triglyceride(TG), lew density lipoprotein chlesterol(LDL-C) and HDL-C,the thickness ratio of intima and tunica media were measured and the expression intensity of vascular endothelial growth factor(VEGF) and vascular cell adhesion molecule 1(VCAM-1) in the thoracic aorta was determined with immunohistochemical staining. Result: After treatment, the serum levels of TC,TG,LDL-C,and the intima/tunica media thickness ratio significantly reduced(P<0.05). There was a significant difference between the groups of high dose and low doseof Huoxue capsule (P<0.05).Positive expressions of VEGF and VCAM-1 found was in the model group, high-dose and low-dose of Huoxue capsule,and the simvastatin one.The expressions mainly existed in the atherosclerotic plaque, but there was no positive expressions of VEGF and VCAM-1 in the blank group. The results of integral optical density suggested that the expressions in treatment groups were decreased compared with the model group(P<0.01, P<0.05). Conclusion: Huoxue capsule can inhibit the expressions of VEGF and VCAM-1 in the thoracic aorta of the rabbits, and this maybe a mechanism in postponing the process of the atheriosclerosis and stabilizing atheromatous plaque.关键词：Huoxue capsule;atherosclerotic;vascular endothelial growth factor;vascular cell adhesion molecule 1
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effect of chelerythrine on experimental ulcerative colitis and possible mechanism. Method: Acetic acid-induced ulcerative colitis(UC) mice model was prepared.60 mice were randomized into model group,positive group,chelerythrine low,medium and high dose groups(25,50,100 mg·kg-1),and a normal group was set up,10 mice was included in each group.The general condition and pathological changes were observed.Myeloperoxidase(MPO) activity and nitric oxide (NO) levels in colon tissue and serum were determined by biochemical assay.Tumor necrosis factor (TNF-α) levels in colon tissue and serum were detected by enzyme-linked immunosorbent assay (ELISA). Result: Compared with the normal group,the injury of colonic tissue in model group was serious, the level of MPO,NO,TNF-α was significantly lower(P<0.01).Compared with the model group,chelerythrine and etiasa significantly alleviated the damage of colonic tissue.The activities of MPO and NO,TNF-α levels were also obviously decreased (P<0.05,P<0.01). Conclusion: Chelerythrine may play its therapeutic role through inhibiting the inflammation due to the generation of NO,TNF-α.关键词：chelerythrine;ulcerative colitis;myeloperoxidase;nitric oxide;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: Observe the protective effects of Bushen Houxue fang(BSHXF) on 6-hydroxydopamine (6-OHDA) type of Parkinson's disease in substantia nigra of injection of 6-OHD rat dopaminergic neurons. Method: Substantia nigra once injection of 4 μL 6-OHDA to model the Parkinson's disease rat, and divide those models into 6 different groups: normal control (0.9% NaCl)model (0.9% NaCl),madopar comparison (20 mg·kg-1 madopar), high (15 g·kg-1 BSHXF), mid (10 g·kg-1) and low (5 g·kg-1) dosage of BSHXF. Each group intragastric administration one time every day for 15 days. Using ethology detection, immunohistochemistry to determine substantia nigra tyrosine hydroxylase, SOD, MDA and GSH-Px, then observe the protective effects of Bushen Houxue fang on DA neurons. Result: ①Group model rotates clockwise (11.27±1.68) lap/min, there is significant difference (P<0.05) among High dosage of BSHXF group (6.35± 1.49 lap/min, madobar group (5.76±1.23)lap/min and model group. ②Madobar and high dosage group compare to model group have increasing activity of substantia nigra (P<0.05), as well as GSH-Px (P<0.01). Meanwhile, high dosage of BSHXF and madobar have similar increase in SOD, GSH-Px and decrease in MDA. ③TH immunohistochemistry result shows BSHXF high dosage and madobar group rat substantia nigra TH have large number of positive neuron, large soma size, significant outgrowth, the comparison of the two groups shows no statistical difference. PD model group has significant decrease in the number of neuron (P<0.01), even disappearing, neuron soma atrophy, little outgrowth. Conclusion: There are protective effects of BSHXF on 6-OHDA type of Parkinson's disease in substantia nigra of rat dopaminergic neurons, its effect mechanism could achieved through anti-oxidative stress and increase level of tyrosine hydroxylase in substantia nigra.关键词：Bushen Houxue fang;dopaminergic neuron;tyrosine hydroxylase;superoxide dismutase;malondialdehyde;glutathione peroxidase
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发布时间：2024-01-04 - 摘要：Objective: To observe the immune promotion and anti-fatigue of effect of Exocarpium citri polysaccharide in mice. Method: KM mice were randomly divided into control group, model group,Zhenqi Fuzheng granule group (10 g·kg-1), E. citri polysaccharide low dose group (23 mg·kg-1), middle dose group (46 mg·kg-1), high dose group (92 mg·kg-1).The groups were gavaged(ig)with drug daily for 30 consecutive days,at dose of 10 mL·kg-1. On 26th day, the delayed-type hypersensitivity animal model in mice was established by sensation of 2,4-dinitro fluoro benzene(DNFB), the control group was treated with the solvent without DNFB,the weight of right and left ears was measured at 24 h after challenged.Since the third week,dexamethasone was injected to all the groups except for control group every two days to establish immunosuppressed mice model, 40 mg·kg-1 dose,five times.Observed spleen index,thymus index at 1 h after last objection. Using swimming and anti hypoxia experiment to observe the anti fatigue effect. Result: The effect of Zhenqi Fuzheng granules group, the polysaccharide groups at 46, 92 mg·kg-1 could enhance the delayed type hypersensitivity(P<0.01,P<0.05), the difference of mouse ear swelling was (6.57± 1.67), (8.29±2.41)mg,respectively.Zhenqi Fuzheng granule group,polysaccharide group at 92 mg·kg-1 could significantly increase the immune suppression of thymus and spleen index in mice(P<0.01, P<0.05). Zhenqi Fuzheng granules group, polysaccharide groups at 23, 46, 92 mg·kg-1 could significantly prolong the swimming time, swimming time was (286±46), (188± 34), (227±28), (291±48)s. Zhenqi Fuzheng granule group, polysaccharide groups at 23, 46, 92 mg·kg-1 could significantly prolong the hypoxia tolerance time of mice(P<0.01, P <0.05). The effect of enhancement of immune function and anti fatigue action showed a manner of dose depent. Conclusion: E. citri polysaccharide can enhance immunity, and have anti-fatigue effect. Polysaccharide is the effective chemical fraction of E. citri.关键词：Exocarpium citri;polysaccharide;immune promotion function;anti fatigue effect
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发布时间：2024-01-04 - 摘要：Objective: To discusse the molecular mechanisms of impaired glucose tolerance(IGT) development induced by long term high-fat diet by investigating the protein expressions of phosphatidyl inositol 3-kinase (PI3K)/protein kinase B (PKB)/glycogen synthase kinase-3β (GSK-3β)/glucose transporter-4 (GLUT-4) in the rat skeletal muscle with IGT. Method: The IGT rat model was replicated by being fed with high fat diet with 45% lipid energy for twenty weeks. The protein concentration of PI3K in the skeletal muscle was examined by immunohistochemical staining, the protein expressions of PKB/GSK-3β/GLUT-4 were detected by Western Blot, and the relative amount of GLUT-4 protein in IGT rats skeletal muscle tissue was tested by Real Time-PCR. Result: Compared to the control group, the protein expressions of PI3K/PKB in skeletal muscle of IGT group were all decreased, the protein expressions of GSK-3β was obvously increased (P<0.01). The mRNA expression of GLUT-4 in IGT group was decreased as compared with the control group, but there was no significant difference. Conclusion: The protein expressions of PKB/GSK-3β/GLUT-4 in skeletal muscle of IGT rats established by long-term high fat diet with 45% lipid energy are obviously changed, which seems to be one of the molecular mechanisms for IGT in this model formation.
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective effect of Mailuoning injection and hormone on spinal cord damage in radiculitis rats and to investigate the underlying mechanism as well as the evidence for substituting Mailuoning injection for hormone. Method: Wistar rats were divided into five groups randomly:normal group, sham group, model group, Mailuoning-treated group and Kangning Ketong-treated group. Filter paper placement was adopted as model method. Radioimmunologic technique was employed for blood interleukin8(IL-8) and thromboxane B2(TXB2) detetion, Nissl's staining method was used for neurons observation and immunohistochemical technique was used to examine the expression of Bax and Bcl-2 in spinal cord. Result: Compared with normal group, neurons degeneration and necrosis were normally observed in model group. Meanwhile, blood concentration of TXB2 and IL-8 was significantly increased (P<0.01), and Bax expression was obviously up-regulated (P<0.05) in model group. Compared with model group, neurons degeneration and necrosis were greatly decreased in Mailuoning-and Kangningketong-treated group, and at the same time, IL-8 and TXB2 concentration as well as Bax-positive expression were decreased, while Bcl-2 protein was remarkably up-regulated, then ratio of Bax/Bcl-2 was obviously decreased (P<0.01). Furthermore, there was no significant difference between the above two groups. Conclusion: The experiment implied that Mailuoning injection could relieve inflammation of spinal cord in radiculit rats and protect spinal cord neurons from apoptosis, which may be one of the mechanisms of Mailuoning injection on reliving lumbocrural pain through replacing hormone in clinical practice.关键词：Mailuoning;radiculitis;spinal cord;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To preparate the phillyrin and forsythiaside A from the n-butanol fraction of Qingqiao of Forsythia suspensa, and to explore their antibacterial activity and free DPPH radical scavenging capacity. Method: The phillyrin and forsythiaside A from the n-butanol fraction of Qingqiao were isolated and purified by means of silica gel column chromatography and recrystallization, etc. The antibacterial activity of phillyrin and forsythiaside A were tested against Escherichia coli, Staphylococcus aureus and Streptococcus pneumoniae by using K-B paper disk method. DPPH assay was employed to assess the antioxidant activity of the phillyrin and forsythiaside A. Result: The diameter of inhibition zone forming by forsythiaside A against E. coli, S. pneumoniae and S. aureus were 12.35, 9.58,12.29 mm, respectively. In vitro antibacterial tests revealed that forsythiaside A exhibited better effect than n-butanol fraction of Qingqiao, but not good as gentamicin, and phillyrin didn't show any activity. Meanwhile, forsythiaside A was more potent against E. coli than other two strains. Morever, DPPH experimental results showed that forsythiaside A had higher free DPPH radical scavenging capacity than the phillyrin. Conclusion: Forsythiaside A showed a higher antibacterial activity and antioxidant activity in vitro than phillyrin, and it may be the main active components of Qingqiao.关键词：phillyrin;forsythiaside A;antimicrobial activity;free DPPH radical scavenging capacity
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发布时间：2024-01-04 - 摘要：Objective: To investigate regulating effect of Yishen Gukang prescription on protein expression of glial fibillary acidic protein(GFAP),c-fos and P substances in dorsal horn of spinal cord in a rat model of osseous metastasis tumor pain. Method: On the basis of osteolytic osseous metastasis model of adenocarcinoma of lung in nude mice,immunohistochemical staining method was adopted to observe expression of GFAP,c-fos and P substances in dorsal horn of spinal cord in a rat model in each group.Image analysis was conducted by image analysis software MIA4.0,five visual fields were chosen from each section to count optical density value of the positive substances expressed in each high magnification visual field under 400 microscope magnification. Result: 100% osseous metastasis occurred in nude mice of the model group,immunohistochemistry data indicated that GFAP,c-fos and P substances in dorsal horn of spinal cord in the model group were over expressed;no osseous metastasis occurred in nude mice of the group with lower dosage of Yishen Gukang prescription and the Chinese drug plus western drug group,immunohistochemistry data indicated that protein expression of GFAP,c-fos and P substances was at a lower level;osseous metastasis occurred in nude mice of the groups with moderate and higher dosage of Yishen Gukang prescription,but proteins expression in relation to pain such as GFAP,c-fos and P substances in dorsal horn of spinal cord in nude mice of these two groups were obviously lower than that of the model group. Conclusion: Yishen Gukang prescription has a certain abirritation by inhibiting protein expression of GFAP,c-fos and P substances in dorsal horn of spinal cord in nude mice.关键词：Yishen Gukang prescription;dorsal horn of spinal cord;osseous medtastasis tumor pain;glial fibillary acidic protein
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发布时间：2024-01-04 - 摘要：Objective: To discuss the improving effect of Huoluo Roujin decoction on hand dysfunction in patients during ischemic stroke recovery period after oral and fumigating-washing administration. Method: Eighty-three patients were randomly divided into the acupuncture group (41 cases) and the observation group (42 cases) by random number table. Patients in acupuncture group received acupuncture treatment. Based on the treatment of the acupuncture group, patients in observation group received Huoluo Roujin decoction by oral and fumigating-washing administration. All patients in two groups received one 6 week periods of treatment. Lindmark score table, Brunnstrom hand movement function evaluation table, the total active motion table (TAM) and Barthel (BI) index scale were used to evaluate the effect after the treatment. Result: The total curative rate in observation group was 92.86%, which is superior to that in acupuncture group 73.17% (P<0.05). After treatment, the single score of Lindmark rating scale hand movement coordination, sensory function, and the combined total score in observation group were superior to those in acupuncture group (P<0.01). The improvement of Brunnstrom hand movement function in both groups were obviously better after treatment, and the observation group had better results (P<0.05). The TAM improvement in observation group was superior to that in acupuncture group (P<0.05). BI index classification in both groups was markedly improved, and the observation group had better results (P<0.05). Conclusion: Based on acupuncture treatment, oral and fumigating-washing administration of Huoluo Roujin decoction could enhance the self-care abilitis of patients by improving the movement, sensory ability and wrist joint activity, reliving the disable degree and promoting functional recovery of hands. The combination of Huoluo Roujin decoction and acupuncture showed a good synergistic effect.关键词：ischemic stroke;hand dysfunction;Huoluo Roujin decoction;acupuncture;recovery
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发布时间：2024-01-04 - 摘要：Objective: To discuss the curative efficacy of Yiqi Qiangxin decoction and influence on myocardial energy expenditure (MEE) and heart type fatty acid binding protein (H-FABP) in treating patients with chronic heart failure (CHF). Method: Eighty patients with CHF were randomly divided into western medicine group (40 cases) and observation group (40 cases) by random number table. Patients in western medicine group received 10 mg benazepril hydrochloride, 50 mg metoprolol tablets, 20 mg spironolactone tablets and 0.125 mg digoxin once daily. Based on the treatment of western medicine group, patients in observation group added 1 dose Yiqi Qiangxin decoction once daily. The patients in both groups received two 4 month periods of treatment. The New York Heart Association(NYHA) cardiac function class, 6 minute walk test (6 MWT) and Lee's heart failure were observed. The left ventricular end-diastolic volume(LVEDV), left ventricular end-systolic volume(LVESV) and left ventricular ejection fraction (LVEF) were detected using echocardiogram, and the MEE was calculated. Levels of serous N-terminal pro-brain natriuretic peptide (NT-proBNP) and heart type fatty acid binding protein (H-FABP) were detected before and after treatment. Data was analyzed by chi-square test of ordered data. Result: The score of NYHA cardiac function class in observation group was superior to that in western medicine group (P<0.05). After treatment, the score of Lee's heart failure in observation group was lower than that in western medicine group, and score of 6 MWT was obviously higher than that in western medicine group (P<0.01). The LVEDV, LVESV and MEE in observation group were obviously lower than those in western medicine group, while LVEF was significantly higher than that in western medicine group (P<0.01). Levels of NT-proBNP and H-FABP in observation group were lower than those in western medicine group (P<0.01). Conclusion: Yiqi Qiangxin decoction could alleviate the clinical symptoms and ameliorate cardiac function in patients with CHF, which may be chieved by regulating their MEE, blocking or reversing the myocardial remodeling.关键词：chronic heart failure;Yiqi Qiangxin decoction;myocardial energy expenditure;heart type fatty acid binding protein
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect and action mechanism of integrated Tongmai Xiaoshuan decoction and conventional western medicine on patients with deep vein thrombosis (DVT). Method: Eighty-four patients with DVT were randomly divided into observation group (42 cases) and western medicine group (42 cases) by random number table. Patients in western medicine group receivedv 5000 U low-molecular-weight heparins calcium injection (hypodermic injection) once daily, 5 Bu batroxobin injection (intravenous drip) every other day, 100 mg bayaspirin enteric-coated tablets once daily after dinner and 10 mL alprostadil injection (intravenous injection). Based on the treatment of western medicine group, patients in observation group added 1 dose Tongmai Xiaoshuan decoction once daily. All patients in both groups received 2 week periods of treatment. The prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), platelet (PLT) and plasma D-two dimer(D-D)level were detected. Changes of pain, swell, sign and venous flow situation were observed, and patency of veins was detected by color Doppler flow imaging. The blood rheology target was tested before and after treatment. Result: The observation group showed a better effect than western medicine group through order information chi-square test (P<0.05). The PT, APTT, FIB, PLT and D-D levels in both groups declined compared with those before after 7 and 14 days of treatment (P<0.01). Besides, FIB and D-D levels in observation group were lower than those in western medicine group at days 7 and 14 after treatment (P<0.01). Moreover, the pain, swell, sign and venous flow situation in both groups were improved at days 7 and 14 afrer treatment compared with those before treatment (P<0.01). Besides, better effects were obtained in the observation group (P<0.01). The whole blood viscosity, plasma viscosity, blood sedimentation and whole blood reductive viscosity in both groups were improved compared with those before treatment (P<0.01). Besides, better effects were obtained in the observation group (P<0.01). Conclusion: Tongmai Xiaoshuan decoction could improve the patency of vein and relieve the symptoms and signs in patients with DVT. Its mechanism of action may be related to ameliorating blood circulation, promoting thrombolytic effects and recanalization of occluded vessels.关键词：deep vein thrombosis;Tongmai Xiaoshuan decoction;plasma D-dimer;hemorheology
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of adjuvant therapy of Yiqi Tongguan decoction on cardiac function, quality of life, restenosis and major adverse cardiac event in patients after percutaneous coronary intervention (PCI) operation. Method: One hundred and ten patients with PCI were randomly divided into western medicine group (53 cases) and observation group (57 cases) by random number table. Patients in western medicine group received anti-coagulant medication of low molecular weight heparin for 1 week, 5 mg clopidogrel hydrogen sulphate tablets once daily for 9 months, 100 mg aspirin enterie ccoated tablets once daily after dinner and 80 mg simvastatin tablets once daily for 2 months after the PCI operation. Based on the treatment of western medicine group, patients in observation group added Yiqi Tongguan decoction 1 dose daily for 3 months. Patients in both groups received 12 month follow-up. The qualities of life in PCI patients were evaluated by Seattle angina questionnaire (SAQ). Echocardiogram was recorded, left ventricular ejection fraction (LVEF) and stroke volume (SV) were calculated. Heart function class was graded by New York heart association (NYHA). Major cardiovascular events of cardiac, cardiac death, unstable angina pectoris, heart failure, PCI operation and bypass were recorded. Result: Composite scores of SAQ scale of level of limitation of motion, anginal stability, seizure of stenocardia and degree of satisfaction with treatment and total scores in observation group were higher than those in western medicine group after treatment (P<0.05 or P<0.01). LVEF and SV in observation group were higher than those in western medicine group (P<0.01). Cardiac function class of NYHA in both groups ameliorated after treatment (P<0.05 or P<0.01), and there was no statistically significant difference from two groups. Occurrence rate of restenosis in observation group was 5.26%, which is lower than that in western medicine group 11.32% with no statistically significant difference. Combined occurrence rate of cardiovascular events in observation group was 14.03%, which is lower than that in western medicine group 35.84% (P<0.01). Conclusion: Adjuvant therapy of Yiqi Tongguan decoction could improve heart function and quality of life, decrease the incidence rate of the main cardiovascular events and restenosis, improve the prognosis and promote the rehabilitation of patients with PCI.关键词：percutaneous coronary intervention;Yiqi Tongguan decoction;Restenosis;cardiovascular events;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of Fupi pills in treating patients with proctoptosis of deficiency of spleen-yang type in Chiese medicial syndrome. Method: Sixty-two patients diagnosed proctoptosis of deficiency of spleen-yang were randomly divided into the Fupi pills group and the control group of 31 cases each. The patients in the control group received submucous-punctiform injection, while the patients in the Fupi pills group received traditional Chinese medicine by oral and fumigating-washing administration. The efficacy was evaluated by comparing the experiment indexes (defecation frequency, defecation time, proctoptosis degree, and defecography, etc.) after 10 day treatment. Result: The curative rate of the Fupi pills group was higher than the control group (P<0.05). The total effective rate of the Fupi pills group was higher than the control group (P<0.05). After 10 day treatment, the total symptom-score in both groups was significantly decreased (P<0.05), which was lower in the Fupi pills group than that in the control group (P<0.05). The indexes such as defecation frequency, defecation time, proctoptosis degree and defecography were improved after treatment (P<0.05), and the improvement was better in the Fupi pills group (P<0.05). The symptom-score of anal pendant expansion was significantly decreased after the treatment (P<0.01), which was lower in the Fupi pills group when comparing with that in the control group (P<0.05). After treatment, the symptom-score of incomplete evacuation was decreased (P<0.01), which was lower in the Fupi pills group (P<0.05). The rest pressure was improved in both groups after treatment (P<0.05), no significant differenceswere shown between two groups. The maximal squeezing pressure had no significant improvement in both groups after treatment (P<0.05). The defecation pressure was significantly decreased in both groups after treatment (P<0.01). Conclusion: Fupi pills together with fuming-washing of Chinese herb medicine have a good effect on rectal prolapse.关键词：rectal prolapse;‘Lanshi Micang’;Fupi pills;fumigating-washing of Chinese herb medicine
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Bushen Tiaojing decoction in treating patients with premature ovarian failure (POF), and its effect on reproductive axis. Method: Ninty-six patients with POF were divided into three groups by a random number table. Patients in traditional Chinese medicine (TCM) group (32 cases) took Bushen Tiaojing decoction, patients in western medicine (WM) group (32 cases)took hormone replacement therapy (HRT), Chinese and Western medicine (CWM) group (32 cases)took Bushen Tiaojing decoction plus HRT. All patients in three groups received six 4-week or six 1-month periods of treatment and 3-months follow-up after treatment. The clinical efficacy, clinical symptom score and serum sex hormones levels [estradiol(E2), follicle stimulating hormone(FSH), luteinizing hormone (LH)]and recurrence rate in three groups were compared before and after treatment, and after 3-months withdrawal. Result: The total effective rate of TCM group, WM group and CWM group were 83.3%, 70% and 93.3% with statistically significant difference among three groups (P<0.05). At the end of treatment and withdrawal of 3 months, clinical symptom scores in TCM and CWM groups were better than that in WM group (P<0.05, P<0.01). At the end of treatment and withdrawal of 3 months, the improvement in blood E2, FSH, LH and FSH/LH levels in TCM and CWM groups were better than that in WM group (P<0.05, P<0.01). The improvement in the situation and time of menses in CWM group were better than that in TCM and WM groups (P<0.05), and recurrence rate in TCM and CWM groups was lower than that in WM group (P<0.05). Conclusion: Bushen Tiaojing decoction can improve the clinical symptoms of patients with POF, restore normal menstruation and regulate serum sex hormones levels. Morover, it had low recurrence rate and fewer adverse reactions. Its mechanism may be achieved by regulating the reproductive axis of Kidney-Tiangui-Chongren-Uterus of traditional Chinese medicine theory.关键词：premature ovary failure;Bushen Tiaojing decoction;hormone replacement therapy;reproductive axis
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发布时间：2024-01-04 - 摘要：Objective: To investigate whether Naoxintong capsule can improve endothelial function in patients with coronary heart disease after percutaneous coronary intervention (PCI) operation. Method: Sixty patients with coronary heart disease after PCI were randomly divided into the observation group and the control group with 30 cases in each group. Patients in two groups received conventional western medicine, the observation group added Naoxintong capsule. All patients in two groups received one 12 weeks periods of treatment and 6 months follow up. The symptoms integral, endothelial function and adverse cardiovascular events in two groups were observed before and after treatment. Result: ①There was no cardiovascular death in two groups, there was one non-fatal acute myocardial infarction (AMI) in each group. The cases of rehospitalization with angina pectoris and acute coronary syndrome (ACS) were lower in the observation group than that in the control group;②The clinical symptom of the observation group were significantly improved after treatment (P<0.01), while only the chest pain, chest tightness in the control group were improved (P<0.05). The improment of palpitations, fatigue and dyspnea in the observation group were better than that in the control group (P<0.01);③After treatment, the -mediated diameter(FMD) was improved, the serum nitric oxide(NO) was increased, the endothelin-1 (ET-1) and ET-1/NO were decreased in two groups (P<0.01);and the improvement in observation group was better than that in control group (P<0.01). Conclusion: Naoxintong capsule can significantly improve clinical symptoms of patients with coronary heart disease after PCI, reduce the incidence rate of adverse cardiovascular events and improve quality of life. Its mechanism may be relevant to the improvement of endothelial function by regulating blood ET-1, NO levels and ET-1/NO ratio.关键词：coronary heart disease;Naoxintong capsule;endothelial function;PCI operation
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of end stage renal disease (ESRD) serum on apoptosis in human umbilical vein endothelial cells and the intervention action by total flavonoids of astragalus in this process. Method: The serums of 22 healthy volunteers and 25 patients with ESRD undergoing regular hemodialysis were collected. Simulating ESRD environment in vitro, human umbilical vein endothelial cells (HUVECs) were selected as the subject. Then they were divided into blank control group (including 10% fetal calf serum), normal control group (including 10% healthy serum), ESRD group (including 10% ESRD serum), low dose group, medial dose group and high dose group with total flavonoids of astragalus (0.5, 1.0, 2.0 g·L-1 total flavonoids of astragalus, respectively). After being culturing for 24 hours, the morphological change of endothelial cells were observed by microscopy, survival rate was detected by MTT test, the expression of C/EBP homologous protein (CHOP) was examined by immunohistochemical staining, the content of 4-hydroxynonenal (HNE) was detected by ELISA, and the morphological change of apoptosis was observed by TUNEL method. Result: Comparing with the normal control group, HUVECs survival rate was lower in ESRD group(P<0.01), CHOP, 4-HNE(P<0.05) and apoptosis index(P<0.01) were increased. The total flavonoids of astragalus could significantly increase the survival rate of HUVECs(P<0.05), and decrease the expression of CHOP(P<0.05). Besides, better results were found in medial dose group than low dose group(P<0.01), but no statistically significant difference were found between high dose group and middle does group. Morever, the total flavonoids of astragalus could decrease 4-HNE and the apoptosis index(P<0.05), and the effect is dose-dependent(P<0.05). Positive correlation were found between CHOP and 4-HNE(P<0.01). Conclusion: ESRD serum could induce HUVECs apoptosis, and the mechanism may be related to endoplasmic reticulum stress. Total flavonoids of astragalus could inhibit endothelial cell apoptosis induced by ESRD serum through alleviating endoplasmic reticulum stress.关键词：total flavonoids of astragalus;end stage renal disease;C/EBP homologous protein;4-hydroxynonenal;apoptosis
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发布时间：2024-01-04 - 摘要：Most Artocarpus (Moraceae) plants are used as traditional folk medicine and have great medical value. In recent years, a large number of prenylated phenolic compounds with significant bioactivities were isolated from Artocarpus, which attracted many attentions of phytochemical researchers. The recent reports on chemical structures and pharmacological effects of chemical constituents from genus Artocarpus were searched by SciFinder and the retrieve results were analyzed. The results showed that more than 70 new compounds were reported from Artocarpus plants in recent five years. Most of these compounds is isoprenylated phenolic compounds, including flavones, flavanones, chalcones, dihydrochalcones, xanthones, aurones, flavan-3-ols, 2-arylbenzofuran derivative, and 1,2-diphenylethylene. These compounds have anti-inflammatory, antibacterial, antioxidant, antitumor, cytotoxic, resistant trypanosomes and plasmodium, inhibiting pancreatic lipase, inhibiting tyrosinase, inhibiting xanthine oxidase, inhibiting-glucosidase and inhibiting melanin production activity. The paper reviews the new chemical compounds of Artocarpus plants and their bioactivities, which would provide references for the development and utilization of Artocarpus plants.关键词：Artocarpus;prenylated flavonoids;non-phenolic constituents;bioactivity
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发布时间：2024-01-04 - 摘要：Premature ovarian failure (POF) is a common gynecological disease characterized with persistent amenorrhea, atrophy of sexual organ, high level follicle-stimulating hormone, high level luteinizing hormone and low level estrogen in women younger than 40 years of age These women usually have normal menarche or delayed puberty, but normal developed secondary sex characters. POF can cause infertility, aphoria and low level estrogen which seriously affects wemon's physical and mental health. Because of the increasing trend in incidence of POF, POF has become a hot and difficult topic of medical study. The main etiology, pathogenesis, syndrome differentiation of POF and traditional Chinese medicine therapies were reviewed in this paper.关键词：premature ovarian failure;traditional Chinese medicine etiology;syndrome differentiation;traditional Chinese medicine treatment
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