最新刊期
卷 22 ， 期 12 ， 2016
- 摘要：Objective: To truly realize quality control of concentration process of Gardeniae Fructus extracting solution, near-infrared spectroscopy (NIR) was used to establish a rapid quantitative analysis model for contents of chlorogenic acid and geniposide, total solid content and density during concentrating process of Gardeniae Fructus extracting solution, which could real-timely reflect its state. Method: Concentration solution samples of Gardeniae Fructus extracting solution in the workshop were collected and on-line NIR were acquired simultaneously. After rejection of abnormal samples, pretreatment of spectra and choice of the corresponding wave band, the multivariate calibration models were respectively established using partial least square method (PLS) between spectra and chlorogenic acid content, geniposide content, total solid content and density, then these models were measured by reference method, which could be used to predicte unknown samples. Result: Correlation coefficients of contents of chlorogenic acid and geniposide, total solid content and density in calibration set were 0.990 5, 0.994 3, 0.986 2 and 0.987 9;correlation coefficients in validation set were 0.987 2, 0.992 5, 0.981 6 and 0.982 3;root mean square errors of cross-valibration (RMSECV) were 0.112, 0.368, 0.148 and 0.013, root mean square error of prediction (RMSEP) were 0.181, 0.302, 0.128 and 0.016, respectively. Relative standard errors of predictions (RSEP) were lower than 6.0%, which can satisfy the quality control standard in production process of traditional Chinese medicine. Conclusion: The method is simple, accurate, reliable and applicable for fast analysis and monitoring of contents of chlorogenic acid and geniposide, total solid content and density in concentration process of Gardeniae Fructus extracting solution from Reduning injection.关键词：near-infrared spectroscopy;Reduning injection;Gardeniae Fructus;concentrated solution;chlorogenic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish a relationship between micromeritic properties of Crataegi Folium blended powder and its compactibility. Method: Taking 50% ethanol extract powder of Crataegi Folium as a model drug, blended powder of Crataegi Folium were obtained by three kinds of drying technology and mixed with microcrystalline cellulose (MCC) and soluble starch at mass ratio of 1:0.5, 1:1 and 1:2, respectively. The compactibility of blended powder was evaluated by monitoring the slope k in regression equation of tensile strength-pressure. Effect of micromeritic properties on compactibility was analyzed by stepwise regression analysis. Result: It was suggested from data of stepwise regression that compactibility was positively correlated to tap density (ρt), cumulative pore volume (PV), moisture content (MC) and critical relative humidity (CRH), and negatively correlated with D50, particle distance (Span), bulk density (ρb), Carr index (CI), Hausner ratio (HR), specific surface area (SSA). Conclusion: Span, ρb, ρt, MC and CRH are proved as the key micromeritic properties involving in compactibility of Crataegi Folium blended powder.关键词：Crataegi Folium;blended powder;micromeritic properties;compactibility;tensile strength;stepwise regression analysis
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发布时间：2024-01-04 - 摘要：Objective: A rapid method was established to judge the end point of blending process of Zhengtian pills by near-infrared spectroscopy, which was in order to improve blending efficiency of this preparation. Method: Near-infrared spectroscopy of blending samples were collected, then blending uniformity was calculated at different time. Principal component analysis (PCA) combined with Mahalanobis distance was used to identify the end point of blending process. HPLC was employed to determine the content of paeoniflorin in order to verify judgment. Result: Blending uniformity of powders increased with blending time extended. After 20 min, blending uniformity reached more than 97%. PCA-Mahalanobis distance results showed that distance remained below 1.0 and kept unchanged basically, suggesting that blending process reached the end point. Quantitative validation results showed that RSD of paeoniflorin content tended to stable after 20 min. Conclusion: Near-infrared spectroscopy technology can be used to determine the end point of blending process of Zhengtian pills.关键词：Zhengtian pills;near-infrared spectroscopy;end point of blending process;Mahalanobis distance;principal component analysis
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发布时间：2024-01-04 - 摘要：Objective: To provide experimental basis for guiding preparation process of Shenling granules by investigating hypnotic efficacy on extraction and purification process. Method: Four groups of extract with different process were prepared. Effects of each extract on synergistic action of pentobarbital sodium with sub-threshold dose and threshold dose on mice were investigated by righting reflex test. Multiple factors model were established with Yin and blood-deficiency induced by cyclophosphamide and hydrocortisone, and sleep deprivation 48 h induced by water platform method. Content levels of amino acid neurotransmitters in brain tissue of rats were determined by HPLC, glutamate (Glu), glycine (Gly), taurine (Tau) and γ-aminobutyric acid (GABA), to name a few. Result: Four groups of extract had different synergistic effect on pentobarbital sodium with sub-threshold dose and threshold dose on mice, especially the group of single volatile oil extraction and alcohol precipitation can significantly shorten sleep latency of mice and prolong sleeping time of mice. Compared with the blank group, contents of Glu, Tau, GABA and ratio of them in brain tissue of the insomnia model rats showed a considerable elevation. While contents of these indicators decreased in different degree after treatment of extract, among them the group of single volatile oil extraction and alcohol precipitation showed more significant, ratio of Glu/(Tau+GABA) was reduced simultaneously. Conclusion: Process route of single volatile oil extraction and alcohol precipitation is the best preparation process of Shenling granules.关键词：Shenling granules;pentobarbital sodium;yin deficiency;insomnia;amino acid neurotransmitters
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation of Platycodonis Radix effervescent tablets. Method: On the basis of single-factor tests, taking effervescent acid-base ratio, the amount of mannitol and effervescent as independent variables, disintegration time and pH as dependent variables. Experiments were based on central composite design, mathematical models were simulated by multiple-linear equation, second-order polynomial equation and triple-order polynomial equation. Optimal formulation of Platycodonis Radix effervescent tablets was predicted by response surface methodology. Result: Optimum formula was as follows:acid-base ratio of 1:1.25 (tartaric acid-NaHCO3), mannitol amount of 52% and effervescent amount of 100% to extract powder. Under these conditions, disintegration time of Platycodonis Radix effervescent tablets was 224 s and pH was 5.32. Conclusion: Platycodonis Radix effervescent tablets can be obtained with good taste, rapidly disintegration and convenience in using. Central composite design-response surface methodology is successfully used to optimize formulation of this preparation with good predictability.关键词：Platycodonis Radix effervescent tablets;Platycodonis Radix decoction;mannitol;disintegration time;tartaric acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize alcohol precipitation technology of Wubie granules intermediates for reducing the daily dose of this preparation. Method: The rationality of alcohol precipitation technology of Wubie granules intermediates was determined by investigating activities of estradiol (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH) of ovariectomized rats and uterus, ovaries of mice. Taking transfer rates of stilbene glucoside, asperosaponin Ⅵ and solid content as indexes, single factor tests were adopted to optimize alcohol precipitation technology. Contents of stilbene glucoside and asperosaponin Ⅵ were determined by HPLC. Result: Optimum alcohol precipitation process was as follows:concentrated extracting liquid of Polygoni Multiflori Radix praeparata group to relative density of 1.10 (60℃), added ethanol until the concentration of ethanol to 60%, standing time of >12 h, concentrated supernatant to relative density of 1.20-1.23 (60℃). Conclusion: Optimal alcohol precipitation technology is reasonable and reliable, which determines by pharmacodynamic screening and single-factor tests. This process can reduce the daily dose and ensure the effectiveness of Wubie granules.关键词：pharmacodynamic test;Wubie granules;alcohol precipitation technology;stilbene glucoside;asperosaponin Ⅵ
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the residues of heavy metals and harmful elements in soil of Province County Jiulong County Gastrodia elata planting base and Gastrodiae Rhizoma, so as to improve the safety evaluation of Gastrodia elate planting. Method: After collecting samples, lead, cadmium and copper contents were determined by atomic absorption spectrometry, and mercury and arsenic contents were detected by atomic fluorescence spectrophotometry, which were adopted as the evaluation criteria for heavy metal content and harmful elements of all samples. Result: The five measured heavy metals and harmful elements in the selected range showed a good linear relationship (r≥0.999), detection limits of lead, cadmium, copper, mercury, arsenic were 8, 0.03, 10, 4, 6 μg·kg-1, with the recovery RSD<2.2%, precision RSD<1.3%, and repeatability RSD<0.9%, indicating that the method is feasible. The established method was used to determine heavy metals and harmful elements of samples held. The results showed that the soil samples conformed to the Soil Environmental Quality Standards (GB15618-1995), and medicine samples were consistent with the Green Industry Standard for Import and Export of Medicinal Plants and Preparations. Conclusion: Soil and herbs in the G. elata planting base conform to GAP standards for lead, cadmium, copper, mercury and arsenic.关键词：soil;Gastrodiae Rhizoma;heavy metals and harmful elements
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发布时间：2024-01-04 - 摘要：Objective: To study the genes related to the synthesis of diterpenoid. Based on the data of transcriptome sequencing, cDNAs encoding 1-deoxy-D-xylulose-5-phosphatereduetoisomerase (DXR) were obtained from the leaves of aseptic seedlings of Rabdosiae Rubescentis Herba. Method: DXR was obtained by reverse transcription PCR. Real-time quantitative PCR was used to detect the relative expression patterns of DXR in different tissues of Rabdosiae Rubescentis Herba. Result: Sequence analysis showed that the full-length cDNA of DXR was 1 500 bp and contains gene open reading frame (ORF) of 1 422 bp encoding 473 amino acids. The theoretical molecular weight was 51.39 kDa and the isoelectric point was predicted as 6.09, suggesting it was a type of hydrophilic protein. The expression pattern of the gene in different tissues was analyzed by Real-time fluorescence quantitative PCR. The results showed the expression of DXR was relatively high in the stem and the lowest in callus. Conclusion: The results will provide a basis for studying the activity and function of DXR from Rabdosiae Rubescentis Herba, and lay a foundation for biosynthesis and gene mining of terpenoids.关键词：Rabdosiae Rubescentis Herba;diterpenoid;1-deoxy-D-xylulose-5-phosphate reductoisomerase
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发布时间：2024-01-04 - 摘要：Objective: To understand the contents of steroidal saponins in Paridis Rhizoma with different growth ages based on numbers of mark of dropped stem bottom. Method: The growing ages of Paridis Rhizoma was identified with the numbers of mark of dropped stem bottom. The contents of polyphyllin Ⅶ, polyphyllin Ⅵ, polyphyllin Ⅴ, polyphyllin Ⅱ and polyphyllin Ⅰ were determined by HPLC. Result: The different batches of samples were divided into the various samples with 3-7, 3-8 and 3-9 years based on the numbers of mark of dropped stem bottom. All tested samples are fulfilled with the require of content limit described in the current Chinese Pharmacopoeia, that the total amounts of polyphyllin Ⅶ, polyphyllin Ⅵ, polyphyllin Ⅱ and polyphyllin Ⅰ are not less than 0.6%. Among the five marker constituents, polyphyllin Ⅶ has the highest amount. The contents of steroidal saponins in the samples have a gradually increasing trend, with the growth years increasing. Conclusion: The mark of dropped stem bottom could be considered as a typical character for the traditional quality evaluation system of Paridis Rhizoma.关键词：Paris polyphylla var. chinensis;rhizoma;mark of dropped stem bottom;growth ages;steroidal saponins
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发布时间：2024-01-04 - 摘要：Objective: To investigate the differences in trace elements between wild and cultivated Hedysari Radix, and analyze the feasibility of trace elements in identifying wild and cultivated Hedysari Radix. Method: The content of trace elements in Hedysari Radix was determined by Atomic Absorption Spectrometry (AAS) and analyzed by one-way analysis of variance, correlation analysis, optimal scaling analysis, cluster analysis and discriminant analysis. Result: There was no significant difference in content of Cu between wild and cultivated Hedysari Radix, but the contents of Fe, Ca, K, Zn, Ni, Na, Mg, Mn, Cr, Li and Co had significant differences between wild and cultivated Hedysari Radix. The contents of Mg, K, Fe, Ca, Cu and Ni in wild Hedysari Radix were higher than those in cultivated samples, but the contents of Zn, Na, Mn, Cr, Li and Co in cultivated Hedysari Radix were higher than those in wild samples. The growth types had highly significantly positive correlation with Fe, Ca, K, Ni and Mg contents, but were significantly negatively correlated with Zn content and had highly significantly negative correlation with Na, Mn, Cr, Li and Co contents. 28 batches of Hedysari Radix samples were divided into two categories:wild Hedysari Radix and cultivated Hedysari Radix. The contents of Fe, Li and Co had Fisher linear relationship with the growth types (wild or cultivated). Conclusion: There was closely relationship between the contents of trace elements and growth types (wild and cultivated) of Hedysari Radix, so there was certain feasibility to identify the wild and cultivated Hedysari Radix based on trace elements.关键词：Hedysari Radix;wild;cultivated;trace elements;identify
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发布时间：2024-01-04 - 摘要：Objective: To determine the contents of trace elements in Hedysari Radix from different places in Gansu province, and provide reliable method for the effective quality control of Hedysari Radix. Method: Wet digestion method was used to process the samples and flame atomic absorption spectrometry was used to determine the contents of Fe, Cu, Ca, Mn, Zn, Mg, Cd, Cr, Co, Na, Li, Ni and K in Hedysari Radix. The analysis of variance and clustering analysis were applied to analyze the results. Result: The elemental contents in Hedysari Radix were as follows: Fe (0.865 9-2.607 1 μg·g-1), Cu (0.185 6-0.467 3 μg·g-1), Ca (2.770 1-3.411 6 μg·g-1), Mn(0.797 6-0.994 4 μg·g-1), Zn (2.732 2-6.482 0 μg·g-1), Mg (88.725 8-99.263 5 μg·g-1), Ni (0.196 6-0.232 4 μg·g-1), Cr (0.059 9-0.068 9 μg·g-1), Co (0.067 0-0.071 6 μg·g-1), Na (20.758 5-27.021 8 μg·g-1), Li (0.341 2-0.423 0 μg·g-1), K (16.695 9-18.753 5 μg·g-1). Cd was not detected in Hedysari Radix. The order for average elemental contents was Mg> Na> K> Zn> Ca> Fe> Mn> Li> Cu> Ni> Co> Cr> Cd from high to low. There was significant difference in contents of Fe, K, Zn, Ni, Na, Mg, Mn and Cr (P<0.05), but the difference was not significant in contents of Cu, Ca, Li and Co in Hedysari Radix from 32 places. The results of clustering analysis showed that 32 samples were clustered into four different groups. The majority of cultivated Hedysari Radix samples were classified into the first group, and wild Hedysari Radix samples were classified into the second group. Conclusion: The differences of trace elements content in Hedysari Radix are not only related to places, but also related to cultivated Hedysari Radix or wild Hedysari Radix.关键词：different places in Gansu province;Hedysari Radix;trace elements;quantitative determination;flame atomic absorption spectrometry
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the quality of Hedysari Radix based on grey incidence degree method. Method: The 31 batches of Hedysari Radix samples were collected in Wudu district, Tanchang county, Minxian county, and Wushan county of Gansu province, and the contents of calycosin and formononetin were determined by HPLC. The content of extract was determined with the method in Pharmacopoeia of China (version 2010). Polysaccharide of Hedysari Radix was determined by ultraviolet spectrophotometry method. Grey incidence degree method was used to establish Hedysari Radix quality evaluation models with the defined relative degree of incidence as the measurement. Result: The results of quality evaluation on 31 batches of Hedysari Radix samples were consistent with those of genuine producing area. The main producing area for Hedysari Radix was Wudu district, and the best quality was from Micang mountain in Wudu district. Few samples of Hedysari Radix in Wudu district had somewhat lower quality, followed by those from Wushan, Minxian, Longxi, and Tanchang of Gansu province. The samples from Xinhua, Pangjia, Chela, and Guanting of Tanchang county had lowest quality, with decayed marks on the head of the herbs. Conclusion: Grey incidence degree method and the established Hedysari Radix quality evaluation models can be used to evaluate the quality of Hedysari Radix.关键词：Hedysari Radix;quality evaluation;grey incidence degree method
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC-DAD method for simultaneous determination of 14 active compounds (baicalin, oroxyloside, wogonoside, jatrorrhizine, copetisine, palmatine, berberine, baicalein, rhein, aloe-emodin, wogonin, emodin, chrysophanol and physcion) in Yiqing capsules. Method: Agilent 1200 HPLC-DAD chromatographic instrument and Agilent ZORBAX SB-C18 chromatographic column (4.6 mm×250 mm, 3.5 μm) were used, with water-acetonitrile-0.2% formic acid in methanol-25 mmol·L-1 KH2PO4 solution as the mobile phase for gradient elution. The flow rate was 0.8 mL·min-1, with the column temperature of 25℃, and DAD detection wavelength was 254, 275, 345 nm respectively. Result: Fourteen compounds showed good linear relationship; the recovery rate was ranged from 97.11%-102.44% with RSD less than 3.0% for all the analytes (n=6). RSD of precision degree was less than 3.0%; and RSD of repeatability testing results was less than 3.0%. Conclusion: This HPLC-DAD method is simple, accurate and reproducible. It could provide scientific basis for the quality control of Yiqing capsules.关键词：HPLC-DAD;Yiqing capsules;quanlity control
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发布时间：2024-01-04 - 摘要：Objective: Study on volatile components and relative content of different fried levels of Zanthoxyli Pericarpium. Method: The volatile componds was extracted by static headspace. The chemical constituents of the volatile componds were detected and analyzed by GC-MS-AMDIS combing with Kováts retention index. Result: A total of 59 compounds was identified in three kinds of fried Zanthoxyli Pericarpium. The sum of relative contents were 98.81%, 98.14%, 97.70%. The componds contents from high to low in three kinds of fried Zanthoxyli Pericarpium were respectively linalyl acetate, linaloo, limonene; the main volatile components of Zanthoxyli Pericarpium pieces were alkenes, alcohols, esters and ketones. The content of allyl compounds in three kinds of Zanthoxyli Pericarpium pieces is the highest, respectively 43.71%, 42.16%, 39.84%; second is alcohols, respectively 25.36%, 24.32%, 24.26%, third is ester compounds, the content were 20.69%, 21.23%, 22.22%; low content is ketones, the contents of which were 8.01% and 8.27%, 7.59%. Conclusion: Different fried levels of Zanthoxyli Pericarpium of volatile components is much the same, but relative content differences.关键词：Zanthoxyli Pericarpium;static headspace;volatile componds;GC-MS;AMDIS;retention index
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发布时间：2024-01-04 - 摘要：Objective: To establish a method to determine the contents of threealkaloids (berberine, jatrorrhizine and palmatine)in Xianglian pills with ionic liquids [BMIM]PF6 as mobile phase additives. Method: The effect of ionic liquid concentration was investigatedby using the Ultimate XB-C18(4.6 mm×250 mm, 5 μm) column for separation, and compared with traditionaltriethylamine (TEA) additives then. Result: Ionic liquid added to the mobile phase couldimprove thepeak pattern of C18separative chromatographiccolumn, improve the separation degree, and the effect was better than that of traditional triethylamine additives. Conclusion: This method can be used for the simultaneous determination of a variety of alkaloids in Xianglian pills.关键词：Xianglian pills;[BMIM]PF6;mobile phase additives;alkaloid
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发布时间：2024-01-04 - 摘要：Objective: To prepare the acetylated Sophora alopecuroides polysaccharide (AC-SAP), and study the conformation characteristics of the modified acetylated S. alopecuroides polysaccharide solution. Method: S. alopecuroides polysaccharide was modified by acetylization, and the modification results were verified by means of the substitution determination method and infrared spectroscopy analysis. The conformation characteristics of acetylated S. alopecuroides polysaccharide was studied by I2-KI reaction and Congo red test. Solution behavior of acetylated S. alopecuroides polysaccharide under different conditions were studied by circular dichroism (CD) spectrum. Result: Acetylated S. alopecuroides polysaccharide was successfully modified and its substitution degree was 0.84. Its solution behavior indicated multiple helixes structure with long side chains and many branches in solution. In addition, its solution conformation could be affected by the concentration, temperature and metalions. Conclusion: Comprehensive research on the solution conformation of successfully modified acetylated S. alopecuroides polysaccharide can provide reference for further research.关键词：Sophora alopecuroides polysaccharide;acetylization;conformation;circular dichroism spectrum
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for quality control of Shujing Fangtong capsule. Method: TLC method was used to qualitatively identify Salvia Miltiorrhiza, Angelica Sinensis Radix, Olibanum and Myrrha in Shujing Fangtong capsule;the contents of paeoniflorin, naringin, neohesperidin and gsalvianolic acid B were simultaneously determined by HPLC, with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution at a detection wavelength of 230 nm. Result: The TLC identification method was distinct and highly specific. Paeoniflorin, naringin, neohesperidin and gsalvianolic acid B showed good linear relationship in ranges of 25.30-506.00 mg·L-1 (r=1.000 0), 12.68-253.60 mg·L-1(r=0.999 9), 15.24-304.80 mg·L-1 (r=0.999 9) and 27.72-554.30 mg·L-1(r=0.999 9) respectively. The average recovery rate of samples was 100.69%(RSD 0.9%), 100.08%(RSD 1.6%), 100.85%(RSD 1.2%) and 100.85%(RSD 2.0%) respectively. Conclusion:This quality control method is simple, accurate and reproducible, which can be used to effectively control the quality of Shujing Fangtong capsule.关键词：Shujing Fangtong capsule;naringin;neohesperidin;salvianolic acid B;quality control
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents in stem barks of Ficus hispida. Method: The constituents were isolated by silica gel, ODS and Sephadex LH-20, and identified based on spectral data and physicochemical properties. Result: The 13 compounds were isolated, and their structures were identified as vanilic acid (1), stigmasterol (2), ursolic acid (3), oleanlic acid (4), syringic acid (5), quercetin (6), rutin (7), stigmasta-5, 22-dien-3β-hydroxy-7-one (8), stigmasta-5-en-3β-hydroxy-7-one (9), 3β-acetoxy-α-amyrin (10), 3β-acetoxy-12-oleanen-11-one (11), oleanonic acid (12), 3β-acetoxy-25-methoxylanosta-8, 23-diene (13). Conclusion: Compounds 5-13 were discovered from this plant for the first time.关键词：Ficus hispida;Ficus;chemical constituent;structural identification
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发布时间：2024-01-04 - 摘要：Objective: To develop a simple UPLC-MS/MS method for simultaneous determination of norephedrine, norpseudoephedrine, ephedrine, pseudoephedrine, methylephedrine, amygdalin and prunasin in rat tissues and to analysis tissue distribution of these compounds in rats after oral administration of Ephedrae Herba-Amygdalus Communis Vas extract. Method: The current method was validated according to the FDA guidelines for the validation of biological sample analysis method. Diphenhydramine hydrochloride and puerarin were selected for quantitative analysis of alkaloids in Ephedrae Herba, amygdalin and prunasin, respectively. Result: Nine active compounds, including 3 pairs of epimers, were separated and quantified within 18 min by a sensitive and reliable UPLC-MS/MS method. Alkaloids in Ephedrae Herba were widely distributed in major tissues. Poor tissue distribution of amygdalin was observed, and prunasin (metabolite of amygdalin) can be detected in tissues except the brain. Contrary to D-amygdalin in vivo, D-prunasin had lower concentration (its concentrations in heart, liver, spleen, lung, kidney were 170.5, 112.8, 98.4, 152.3, 381.7 ng·g-1) in tissues by comparing with L-prunasin (its concentrations in heart, liver, spleen, lung, kidney were 906.4, 652.3, 177.4, 500.9, 2 060.4 ng·g-1). Conclusion: The method is successfully applied to tissue distribution study in rats after intragastric administration of Ephedrae Herba-Amygdalus Communis Vas extract. Reduction of D-prunasin in tissues may be the reason of toxicity antagonism in vivo. High concentration of alkaloids in Ephedrae Herba, amygdalin and prunasin are found in lung, which may be correlated with its synergistic anti-asthmatic effects.关键词：Ephedrae Herba-Amygdalus Communis Vas;herbal pair;amygdalin;prunasin;ephedrine;tissue distribution
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of salvianolic acids compatible with borneol at different concentration on absorption rate constant (Ka) and apparent absorption coefficient (Papp) of salvianolic acid B, lithospermic acid and rosmarinic acid in rat small intestine. Method: An in situ intestinal perfusion model was employed to investigate absorption of salvianolic acids in rat small intestine. HPLC was used to determine contents of salvianolic acid B, lithospermic acid and rosmarinic acid, mobile phase was 1% glacial acetic acid water solution-1% glacial acetic acid methanol solution, detection wavelength was 286 nm, flow rate was 0.8 mL·min-1. Result: Compatibility of salvianolic acids with different concentration of borneol, both Ka and Papp were increased, enhancing effect of borneol with the concentration of 100 mg·L-1 was optimum. Ka of salvianolic acid B, lithospermic acid and rosmarinic acid in borneol with the concentration of 100 mg·L-1 was 2.31, 2.33, 3.38 times as long as the blank group. Conclusion: After compatibility of salvianolic acids with different concentrations of borneol, there is significant promoting effect of salvianolic acid B, lithospermic acid and rosmarinic acid in absorption of all segments of intestine. The capacity of enhancing effect is influenced by the concentration of borneol. When the concentration of borneol increases, the enhancing effect of salvianolic acids in intestinal absorption increases, but it significantly decreases after the concentration of borneol increases to some degree.关键词：Salvianolic acid;borneol;in situ intestinal absorption;single pass intestinal perfusion;salvianolic acid B;lithospermic acid;rosmarinic acid
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Shenling Baizhu particles on intestinal epithelial tight junctions in rats with functional diarrhea. Method: The functional diarrhea spleen deficiency syndrome model in rats was established by using high lactose+small platform standing method. the rats were divided into normal group, model group, pinaverium bromide group, Shenling Baizhu particle high, medium and low dose groups. Shenling Baizhu particle groups were administrated with Shenling Baizhu particle at doses of 3.212, 1.606, 0.803 g·kg-1, respectively, and pinaverium bromide group was given pinaverium bromide of 0.0125 g·kg-1; meanwhile, normal group and model group were given physiological saline intragastrically for 14 successive days. The diamine oxidase(DAO) and tumor necrosis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay(ELISA) method; the zonula occludens-1 (ZO-1), Claudin-1, Occludin mRNA expressions were detected by real-time fluorescent quantitative polymerase chain raction(qPCR) and ZO-1, Claudin-1 protein expressions were detected by immunohistochemistry method. Result: Compared with the normal group, the model group showed significant increases in serum DAO and TNF-α content in colonic mucosa (P<0.01), and decreases in ZO-1, Occludin, Claudin-1 mRNA expressions (P<0.01) and ZO-1, Occludin protein expression (P<0.01); compared with the normal group, Shenling Baizhu particle groups showed decreases in serum DAO and TNF-α content in colonic mucosa (P<0.01), and up-regulations in ZO-1, Occludin, Claudin-1 mRNA expressions (P<0.01) and ZO-1, Occludin protein expression P<0.05, P<0.01) in functional diarrhea model rats. Conclusion: Shenling Baizhu particles can regulate tight junction protein expression in colonic mucosa, and intestinal mucosal mechanical barrier, which may be one of mechanisms of treating functional diarrhea.关键词：Shenling Baizhu particles;functional diarrhea in rats;zonula occludens-1;Occludin;Claudin-1
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发布时间：2024-01-04 -
Protective Effect of Zhizi Dahuang Tang on Carbon Tetrachloride Induced Acute Hepatic Injury in Mice
摘要：Objective: To investigate the protective effect of Zhizi Dahuang Tang (ZDT) on carbon tetrachloride (CCl4)-induced acute hepatic injury in mice. Method: A total of 72 KM mice were randomly divided into normal control group, model group, bifendate pill group, ZDT low-dose group, ZDT medium-dose group and ZDT high-dose group, with 12 mice in each group. Bifendate pill (0.2 g·kg-1) or different doses (6, 12, 24 g·kg-1) of ZDT were given to corresponding groups by gavage for consecutively 5 days. The same volume of saline was infused in normal control group and model group. All groups of mice were intraperitoneally (ip) injected with CCl4, except for normal control group. Mice were sacrificed to collect their blood and liver specimens. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver superoxide dismutase (SOD) and malondialdehyde (MDA) were detected. Histological examination was performed using hematoxylin-eosin staining and light microscopy, and Bax, Bcl-2 and Cleaved-Caspase-3 were detected for apoptosis by using Western blot. Result: Compared with the normal group, the normal group showed significant increases in serum AST and ALT activities (P<0.05) and MDA content, decreases in SOD activity(P<0.05), hepatocyte deformation and necrosis, up-regulations in Bax and Cleaved-Caspase-3 expressions and down-regulation of Bcl-2 expression; compared with the model group, ZDT groups showed significant decreases in serum ALT activity (P<0.05), and ZDT medium and high-dose groups showed significant decreases in serum AST activity(P<0.05), and all of the groups showed significant reduction in MDA content (P<0.05) and rise in SOD activity (P<0.05). According to hepatic tissue sections, all of the treatment groups showed alleviations in hepatocyte deformation and necrosis, particularly the high-dose group, and all of ZDT groups showed down-regualtions in hepatic tissue Bax, Cleaved-Caspase-3 expression, and up-regulation in Bcl-2. Conclusion: ZDT exhibits a protective effect on CCl4-induced hepatic injury by resisting anti-lipid peroxidation and suppressing the apoptosis of liver tissues.关键词：Zhizi Dahuang Tang;acute hepatic injury;carbon tetrachloride;lipid peroxidation;apoptosis- 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Chaihu Shugan decoction on the expression of voltage-gated potassium channel 4.2(Kv4.2) and Kv channel-interacting protein 1(KChIP1) in a rat model of chronic temporal lobe epilepsy induced by pilocarpine. Method: Totally 80 SPF Wistar rats were injected with pilocarpine intraperitoneally, and those with spontaneous recurrent seizures after latent period were selected into the model. All of the modeled rats were randomly divided into five groups: model group, potassium channel agonist retigabine treatment group (12.5 mg·kg-1·d-1), and high, medium and low-dose Chaihu Shugan decoction groups (2.5, 5, 10 g·kg-1), with 10 rats in each group. Another 10 normal rats were included in the normal group and administrated with saline same as model group. All groups were treated for 8 weeks. To evaluate the effect on seizure frequencies, the behavior of the rats were continuously recorded by digital camera during the study. The expression of the voltage-gatedpotass iumchannel 4.2(Kv4.2) and KChIP1 in hippocampus and temporal lobe were detected through immunohistochemistry and Western blot. Result: After 8 weeks of treatment, retigabine group and Chaihu Shugan decoction groups (medium and high doses) showed significantly low seizure frequencies than model group (P<0.05). Compared with normal group, the expressions of the two proteins in model group reduced in hippocampus (P<0.05); retigabine group and Chaihu Shugan decoction group (medium and high doses) showed remarkably higher Kv4.2 expression (P<0.05), and retigabine group was similar with Chaihu Shugan decoction low and high-dose groups. Conclusion: Chaihu Shugan decoction had a significant effect on Kv4.2 and KChIP1 in CA1 region of hippocampus and temporal lobe in rats with chronic temporal lobe epilepsy.关键词：Chaihu Shugan decoction;temporal lobe epilepsy;voltage-gated potassium channel 4.2;kv channel-interacting protein 1
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of baicalin on Phosphatidylinositol 3-kinase/threonine protein kinase/nuclear factor-κB (PI3K/Akt/NF-κB) signaling pathways in cell inflammation models of human colon carcinoma epithelial cell line (HT-29). Method: Tumor necrosis factor-α (TNF-α) and lipopolysaccharides (LPS) were used to induce HT-29 cells and establish cells inflammation models. This experiment was divided into six groups: complete medium was used in the blank control group, without any drug intervention during experiment, the cells in model control group were incubated with hTNF(20 μg·L-1) for 12 h, then incubated with LPS (1 mg·L-1) for 15 h, the cells in positive medicine group were also incubated with salazosulfapyridine (SASP, 500 μmol·L-1) for 24 h on the basis of the processing method in model group, cells in baicalin groups were incubated with different doses (1, 10, 100 μg·L-1) of baicalin for 24 h on the basis of the processing method in model group. MTT assay was used to detect the effect of baicalin (1, 10, 100 μg·L-1) on cell growth, Western blot was used to measure the effect of baicalin (1, 10, 100 μg·L-1) on protein expression levels of PI3K, Akt and NF-κB, enzyme linked immunosorbent assay (ELISA) was used to measure the effect of baicalin (1, 10, 100 μg·L-1) on TNF-α, interleukin (IL)-6 levels in cell supernatant. Result: As compared with the model group, the secretion levels of TNF-α, IL-6, IL-8, and IL-1 in the cell supernatant were reduced in baicalin (1, 10, 100 μg·L-1) groups and SASP (500 μmol·L-1)group (P<0.05), and the combined application of these two medicines reduced more secretion of TNF-α, IL-6, IL-8, and IL-1 levels in the cell supernatant (P<0.01). As compared with the model group, the PI3K protein phosphorylation levels, phosphorylated Akt levels and the activated NF-κB levels were reduced in baicalin groups (1, 10, 100 μg·L-1), and SASP (500 μmol·L-1) group (P<0.05). As compared with the model group, the cyclooxygenase-2 (Cox-2), β-catenin, Caspase-9 and FasL protein expression levels were reduced in baicalin (1, 10, 100 μg·L-1)groups and SASP (500 μmol·L-1) group (P<0.05). Conclusion: Baicalin can reduce HT-29 cell inflammation, inhibit PI3K phosphorylation, down-regulate Akt activation, inhibit the activation of NF-κB, and thus inhibit the secretion of TNF-α, IL-6 and other inflammatory factors to play its anti-inflammatory effect. It suggested that baicalin may play its anti-inflammatory effect by inhibiting the activation of Akt and inhibiting NF-κB signaling pathways.关键词：baicalin;inflammation;serine/threonine protein kinase;nuclear transcription factor-κB
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发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-osteolytic metastasis effects of Zuogui Wan for breast cancer. Method: Balb/C Nu mice were used to establish in vivo experimental bone metastasis models by left ventricle injection of human breast cancer MDA-MB-231 cells. The mice in the experiment was divided into three groups: blank group, Zuogui Wan groups (21, 42 g·kg-1). The cytokeratin 19(CK19) in bone marrow were detected by nest PCR assay. The bone marrow-derived macrophages (BMMs) were in vitro isolated as osteoclast precurosors, and soluble receptor activator of nuclear factor-κB ligand(sRANKL) was exogenously added to simulate the activation of osteoclasts under tumor conditions, and then the effects of Zuogui Wan on activation of osteoclasts, Cathepsin K secretion levels and bone slices absorption were observed. Western blot assay was used to detect its effect on RANK expression levels in BMMs. Result: As compared with the blank group, Zuogui Wan(21, 42 g·kg-1) exhibited significant inhibition on human CK19 expression levels in bone marrow tissues of animals with breast cancer bone metastasis (P<0.01). In vitro serum pharmacological studies showed that Zuogui Wan significantly inhibited RANKL-induced osteoclastogenesis in murine BMMs(P<0.05) and significantly inhibited the secretion of Cathepsin K in RANKL-induced osteoclasts (P<0.05). Bone slices experiment indicated that, the number and area of bone slices resorption pits in 10% Zuogui Wan group were significantly lower than those in normal group (P<0.05). Western blot assay indicated that medicated serum of Zuogui Wan could significantly inhibit the expression of RANK in RANKL-induced osteoclasts. Conclusion: Zuogui Wan have significant anti-osteolytic metastasis effects for breast cancer cells, and the mechanism may be associated with inhibiting RANKL/RANK activation.关键词：Zuogui Wan;osteolytic metastasis;breast cancer;nest PCR;cytokeratin 19;soluble receptor activator of nuclear factor-κB ligand;cathepsin k
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Huangqi San formula (HQS) drug containing serum on osteoblasts (Ob) functions and related genes of diabetic osteoporosis under the condition of high glucose, and discuss the therapeutic mechanism of HQS on diabetic osteoporosis. Method: Ten 5-week-old healthy clean grade Wistar rats were randomly divided into the normal diet group and medicine group. The drug containing serum of HQS and control serum were prepared by serum pharmacology means, four neonatal healthy clean grade SD rats were used for primary culture of Obs. The cells were isolated by enzyme digestion and induced from skull bone of these SD neonatal rats. Cell counting kit (CCK-8) assay was used to detect the effect of drug containing serum (four different concentrations: 5%, 10%, 20% and 30%) on cell proliferation under high glucose condition (25.5 mmol·L-1). The serum concentration which was optimal for cells proliferation was then selected. Real-time PCR and Western blot assay were used to detect the effects of HQS drug containing serum on the expression levels of osteogenic genes osteopontin (OPN), osteocalcin(OC), alkaline phosphatase (ALP), pancrease-bone related gene osteoprotegrin (OPG), and Fork transcription factor 1 (FoxO1). Result: In cell proliferation experiments, 10% and 30% serum concentration groups had significant higher results as compared with their blank serum groups (P<0.05), and 20% serum concentration group also had significant higher results (P<0.01). In the Real-time PCR results, 5% serum concentration group could significantly down-regulate OPN and OPG mRNA expression levels as compared with the blank control group (P<0.05). 10% serum concentration groups could significantly up-regulate OC and ALP mRNA expression levels (P<0.05), and also significantly increase OPN mRNA expression (P<0.01), but significantly down-regulate the OPG and FoxO1 mRNA expression levels as compared with the blank serum groups (P<0.01). Under 20% serum concentration, the differences were identical with those under 10% serum concentration, especially the differences in OPN, OPG and FoxO1 mRNA expression levels were more significant under 20% serum concentration. 20% serum concentration group could significantly up-regulate OC and OPN protein expression levels as compared with the blank control serum group (P<0.01), while OPG protein expression was significantly down-regulated (P<0.01). Conclusion: The therapeutic mechanism of HQS may be related to its proliferation effects on osteoblasts under high glucose condition and its regulatory effects on the expression levels of OPG and FoxO1.关键词：Huangqi San;osteoblasts;osteogenic genes osteopontin;osteocalcin;alkaline phosphatase;pancrease-bone related gene osteoprotegrin;Fork transcription factor 1
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发布时间：2024-01-04 - 摘要：Objective: To investigate whether hederagenin (HG) could inhibit the invasion and metastasis of colorectal cancer by epithelial-mesenchymal transition (EMT) pathway. Method: MTT was used to determine the proliferation of transforming growth factor (TGF)-β1-induced colorectal cancer cells SW480 with different doses of HG treating for 24 h. Transwell assay was used to determine the inhibitory effects of HG on cell invasion in SW480 cells. Western blot and real-time fluorescent quantitative polymerase chain reaction(qPCR) assay were used to observe the expressions of EMT markers such as E-cadherin, N-cadherin, Vimentin, Snail, and invasion and metastasis markers such as signal transducer and activator of transcription (STAT)-3, matrix metalloproteinase (MMP)-9, reversion-inducing cysteine-rich protein with Kazal motif (RECK) and MMP-14. Result: Here we found that HG inhibited the proliferation of SW480 cells which induced by TGF-β1, influenced EMT and reduced the invasion ability. Furthermore, HG reduced the expressions of EMT markers including N-cadherin, Vimentin, Snail and invasion and metastasis markers including STAT-3, MMP-9, MMP-14, enhanced the expressions of E-cadherin and RECK (P<0.05). Conclusion: HG inhibited the invasion and metastasis of SW480 cells by influencing EMT pathway.关键词：hederagenin;epithelial-mesenchymal transition;invasion;colon cancer
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Shenqi Fuzheng injection on proliferation and apoptosis of basal-like type human breast cancer MDA-MB-231 cells, and investigate the treatment mechanism of Shenqi Fuzheng injection for triple negative breast cancer. Method: The human breast cancer MDA-MB-231 cells cultured in vitro were divided into Fuzheng injection groups (six groups with different concentrations of Shenqi Fuzheng injection) and blank group. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of Shenqi Fuzheng injection on proliferation of MDA-MB-231 cells at 24, 48 and 74 h. Flow cytometry (FCM) was used to detect the cell cycle distribution and apoptosis of MDA-MB-231 cells. Real-time PCR and Western blot assay were used to detect the effect of Shenqi Fuzheng injection on p53 up-regulated modulators of apoptosis (PUMA) in MDA-MB-231 cells. Result: Shenqi Fuzheng injection could inhibit the proliferation of MDA-MB-231 cells in a concentration-dependent manner, and the inhibitory effect was most obvious at 48 h in vitro culture. Shenqi Fuzheng injection blocked cell cycle at S phase of the MDA-MB-231 cells, and thus induced apoptosis of MDA-MB-231 cells. Real-time PCR and Western blot assay showed that Shenqi Fuzheng injection could up-regulate PUMA expression of MDA-MB-231 cells. Conclusion: Shenqi Fuzheng injection can significantly inhibit proliferation and block cell cycle at S phase of the MDA-MB-231 cells, thus induce apoptosis of the cells, and the mechanism may be associated with up-regulating PUMA mRNA expression.关键词：Shenqi Fuzheng injection;basal-like type breast cancer;MDA-MB-231 cells;p53 up-regulated modulators of apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Zhali Nusi Fang(ZLNF) intervening bone marrow mesenchymal stem cells (BMSCs) transplantation on synaptic structural plasticity in rats after cerebral ischemia reperfusion injury (CIRI). Method: SD rats were randomly divided into sham-operated group, model group, ZLNF group, BMSCs transplantation group and ZLNF combined with BMSCs groups. Middle cerebral artery occlusion (MCAO) model was duplicated with nylon thread, BMSCs were cultured and amplified by the whole bone marrow adherence method. Drugs were given to the rats by intragastric administration (14.6 g·kg-1·d-1), BMSCs suspension solution was transplanted into rat brain through carotid artery. Brain was taken out at 1, 3, 7, 14 days after transplantation. The changes of neuronal synaptic ultrastructure were observed by transmission electron microscopy, synaptophysin(SYN) and postsynaptic density protein-95(PSD-95)protein expressions were detected by Western blot. Result: The structure of synapses in sham-operated group was integrated, the presynaptic membrane and postsynaptic membrane were clear, the synaptic cleft was visible, and a lots of synaptic vesicles distributed equably. While in model group, the quantity ofsynapses were decreased, the structure was destroyed and the synaptic cleft was dim, the amount of synaptic vesicles was decreased and even disappeared. The quantity of synapses was increased, the structure was close to the normal, and the concave and U form synapses could be seen in ZLNF group, the synaptic vesicles were increased at 3, 7, 14 days in ZLNF group, the synaptic cleft became wider at 14 dayin ZLNF group, the changes of neuronal synaptic ultrastructure in transplantation groups were in keeping with ZLNF group, the damage of synapses in combination groups was significantly lower, the synapses increased, and many concave synapses could be seen at 7, 14 days in combination groups, the synaptic vesicles were significantly increased. The SYN, PSD-95 expressions in model groups were obviously decreased when compared with the sham-operated group (P<0.01). Compared with the model group, the SYN expression was increased at 7 day group in ZLNF and transplantation and in all the combination groups (P<0.01), the PSD-95 expression was increased at 3, 7, 14 days in ZLNF and transplantation and in all the combination groups (P<0.01). Compared with the transplantation groups, the SYN, PSD-95 expressions were increased in all the combination groups (P<0.01, P<0.05), the PSD-95 expression was decreased at 3 day in ZLNF group (P<0.01). Compared with the ZLNF group, the SYN, PSD-95 expressions were increased in all the combination groups (P<0.01). Compared with the same time groups, the SYN expression at 3 day group in ZLNF, transplantation and combination groups was lower than other groups (P<0.01), and the PSD-95 expression in all 7 day groups was higher than other groups (P<0.01), the PSD-95 expression in all 14 day groups was higher than any 3 day group (P<0.01, P<0.05), the SYN expression at 14 day was lower than that at 7 day in ZLNF, transplantation group (P<0.01). Conclusion: ZLNF could increase the synaptic structural plasticity obviously after the transplantation of BMSCs to CIRI, the effect was superior when using the combination of them, and its mechanism may be related to the regulation of the dynamic expressions of SYN and PSD-95.关键词：cerebral ischemia reperfusion;Zhali Nusi fang;bone marrow mesenchymal stem cells;synaptic structural plasticity
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Hui medicine Zhali Nusi Fang (ZLNF) in intervening the impact of bone marrow mesenchymal stem cells (BMSCs) transplantation on neuronal axons regeneration in rats after cerebral ischemia reperfusion injury (CIRI). Method: SD rats were divided randomly into Sham-operated group, model group, ZLNF group, BMSCs transplantation group and ZLNF-BMSC combination group; middle cerebral artery occlusion (MCAO) model was duplicated with nylon thread, BMSCs were cultured and amplified by the whole bone marrow adherence method; drugs were given to the rats by intragastric administration (14.6 g·kg-1·d-1), BMSCs were transplanted into rat brains through carotid artery; rat brains were taken out at 1, 3, 7, 14 days after transplantation; growth associated protein-43 (GAP-43), neurofilament protein-200 (NF-200), myelin associated glycoprotein (MAG) and Nogo-A protein expression were detected by Western blot. Result: The GAP-43, MAG, Nogo-A in the model groups were obviously increased than the sham-operated groups (P<0.01), and with significant decrease in NF-200 (P<0.01); compared with the model groups, the GAP-43 at 3, 14 days in the ZLNF group, at 3, 7, 14 days in the transplantation group and the combination group were increased (P<0.01, P<0.05), the NF-200 in the ZLNF group, the transplantation group and the combination group were increased (P<0.01), but with decreases in MAG and Nogo-A (P<0.05); compared with the transplantation groups, the GAP-43 at 14 day, the NF-200 at 7 day and MAG at 1, 3, 14 days in the ZLNF group were decreased (P<0.01, P<0.05), and the Nogo-A at 3 day in the ZLNF group was decreased (P<0.01) but increased at 7 day (P<0.05), the GAP-43 at 1, 14 days and the NF-200 in the combination group were increased (P<0.01), the NF-200 at 7 day and MAG, Nogo-A in the combination group were decreased (P<0.01); compared with the ZLNF group, the GAP-43 at 3, 7, 14 days and the NF-200 at 14 day in the combination group were increased(P<0.01), the MAG, Nogo-A in all of the groups were decreased (P<0.01); in the intra-group comparison, the GAP-43 and MAG at 7 day in the model group were increased compared with that at 3, 14 days (P<0.01, P<0.05), the NF-200 at 3 day in the model group was increased compared with that at 1 day, the Nogo-A at 3 day reached peak (P<0.01), the GAP-43 at 14 day group in ZLNF, transplantation and combination groups were higher than that at 7 day (P<0.01), the NF-200 at 7 day in ZLNF and transplantation groups were higher than that at 3, 14 days (P<0.01, P<0.05), the NF-200 at 3 day in the combination group was relatively low (P<0.01) and later gradually higher (P<0.05), the MAG, Nogo-A in ZLNF, transplantation and combination groups were increased first and then decreased. Conclusion: Hui medicine ZLNF can obviously promote the neuronal axons regeneration after the transplantation of BMSCs for cerebral ischemia reperfusion injury, with a significant effect in their combination, and its mechanism may be related to dynamic expressions of GAP-43, NF-200, MAG and Nogo-A after the intervention.关键词：cerebral ischemia reperfusion;Zhali Nusi fang;bone marrow mesenchymal stem cells;neuronal axons regeneration
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Hui Medicine Zhali Nusi Fang(ZLNF) intervening bone marrow mesenchymal stem cells(BMSCs) transplantation on Adhesion molecules and neuronal nuclei(NeuN) expression in rats after cerebral ischemia reperfusion injury(CIRI). Method: SD rats were divided randomly into Sham-operated, model, ZLNF, BMSCs transplantation and ZLNF combined with BMSCs groups;middle cerebral artery occlusion(MCAO) model was duplicated with nylon thread, BMSCs were cultured and amplified by the whole bone marrow adherence method;drugs were given to the rats by intragastric administration(14.6 g·kg-1·d-1), BMSCs suspension solution were transplanted into brain through carotid artery;rat neurological scores(NS) were evaluated and brain was taken out at 1, 3, 7, 14 days after transplantation;vascular cell adhesion molecular(VCAM-1) and integrin α4mRNA were detected by real time-PCR and immunohistochemical method to measure the expression of NeuN. Result: The NS and NeuN in model groups were obviously decreased than the sham-operated groups(P<0.01), and the expression of VCAM-1 and integrin α4 mRNA were significantly increased(P<0.01);compared with the model groups, the NS at 1, 3, 14 days in ZLNF and at 3, 7, 14 days in transplantation groups and in all combination groups were increased(P<0.01, P<0.05), the NeuN in all of the groups were increased(P<0.01), the VCAM-1 and integrin α4 mRNA in all of the groups were decreased(P<0.01);compared with the transplantation groups, the NS at 7 day in ZLNF group was decreased(P<0.05) and were significantly increased at 1, 3, 14 days in combination groups(P<0.01), the VCAM-1 mRNA at 1, 3 days in ZLNF groups were decreased(P<0.01), and the integrin α4 mRNA at 1, 7, 14 days in ZLNF groups were decreased(P<0.01), the VCAM-1 and integrin α4 mRNA in all of the combination groups were decreased(P<0.01), the NeuN in all of the ZLNF groups and combination groups were increased(P<0.01);Compared with the ZLNF groups, the NS and NeuN in combination groups were obviously increased and the expression of VCAM-1 and Integrin α4 mRNA were significantly decreased(P<0.01). Compared with the same group, the changes of NS, NeuN and VCAM-1 mRNA at all groups were remarkable in 7 day group, and were improved in 14 days group, the changes of integrin α4 mRNA were remarkable in 3 day group, and can improved in 14 days group. Conclusion: The neurological function and neurons were damaged to some degree after cerebral ischemia;both ZLNF and BMSCs transplantation can improved the neurological function and the condition of neurons, the effect was superior by the combination of them, and its mechanism may be related to the regulation of the dynamic expression of VCAM-1 and Integrin α4.关键词：cerebral ischemia reperfusion;Zhali Nusi fang;bone marrow mesenchymal stem cells;vascular cell adhesion molecular-1;integrin α4;neuronal nuclei
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical efficacy of Wendan Tang on urinary microalbumin/creatinine ratio in patients with resistant hypertension, and clarify its protective effect on renal function. Method: The 90 patients with resistant hypertension from chronic diseases outpatient and inpatient of our hospital during September 2012-September 2014 were randomly divided into treatment group and control group. Blood pressure, traditional Chinese medicine(TCM) syndromes, and biochemical indicators were recorded before and after treatment for both groups. Urinary microalbumin/creatinine ratio levels were detected and analyzed before and after treatment. Result: In respect of blood pressure, the systolic blood pressure and diastolic blood pressure in treatment group were lower than those in the control group, with statistical difference (P<0.05). In respect of TCM syndromes, the results showed that was a total effective rate of 84.1% in the treatment group (44 cases), while in the control group the total effective rate was 58.1%, with significant differences between two groups (P<0.05). In respect of biochemical indicators, urinary microalbumin/creatinine of the biochemical indicators was significantly decreased in the treatment group (P<0.05). After treatment, renal dysfunction was present in treatment group significantly lower than the control group (P<0.05). Conclusion: Wendan Tang has significant effects in reducing the blood pressure for resistant hypertension patients, improving clinical symptoms, and reducing toxins in the blood and urinary microalbumin/creatinine, with a protective effect on renal function, so it is worthy of clinical application.关键词：Wendan Tang;resistant hypertension;urinary microalbumin/creatinine ratio;Professor DENG Tie-tao
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发布时间：2024-01-04 - 摘要：Objective: To observe Qingfei Huoxue methods on serum interleukin (IL)-6, serum interleukin (IL)-8, tumor necrosis factor (TNF)-α, serum high sensitivity (hs-CRP) and plasma fibrinogen (Fg) and exhaled nitric oxide (FeNO) of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD), so as to study the efficacy on AECOPD. Method: Totally 64 cases of AECOPD patients was randomly divided into control and treatment groups. Control group was given routine western medicine therapy, while treatment group was provided Qindan decoction in addition to the routine western medicine therapy. Their clinical effects and changes in serum IL-6, and IL-8, and TNF-α, and hs-CRP, and ESR and the plasma Fg and FeNO levels before and after the treatment were observed. Result: Efficiency of the treatment group was 90.63% (29/32), which was higher than 71.88% (23/32) in the control group (P<0.05), before the treatment, patients of both groups showed no statistically significant difference in IL-6, IL-8, TNF-α, hs-CRP, ESR and Fg and FeNO levels in plasma, after the treatment, the two groups showed no significant change in IL-6 and ESR, with no statistically significant difference, but significant changes in IL-8, TNF-α, hs-CRP and plasma Fg and FeNO levels (P<0.05); however, the treatment group was significantly inferior to the control group (P<0.05). Conclusion: Qindan decoction has a certain effect in regulating inflammatory responses of patients with AECOPD and reducing their serum IL-8, TNF-α, hs-CRP and plasma Fg, FeNO levels.关键词：Qingfei Huoxue methods;Qindan decoction;chronic obstructive pulmonary disease;interleukin-6;interleukin-8;tumor necrosis factor-α;serum high sensitivity;plasma fibrinogen
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发布时间：2024-01-04 - 摘要：Objective: To observe the treatment efficacy of Qingre Jiedu Huoxue Tongluo formula combined with Fufang Huangbai liquid on the diabetic foot. Method: The 178 patients with diabetic foot from the Department of Endocrinology of the Third Affiliated Hospital of Qiqihar Medical University from April 2014 to May 2015 were randomly divided into the observation group (89 cases) and the control group (89 cases). In the observation group, patients were treated with Qingre Jiedu Huoxue Tongluo formula by orally taking and Fufang Huangbai liquid by topical application for three weeks, in the control group, patients were treated with topical Kangfuxin for three weeks. Then the changes of tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF) and interleukin (IL)-6 levels in serum, improvement of clinical symptoms and the changes of blood and nerve indexes were observed in both groups, the correlation between the blood and nerve indexes and related cytokines was analyzed by the method of biological statistics. Result: There was statistically significant difference in therapeutic effect between two groups (P<0.01). There were statistically significant differences in the blood flow rate of dorsalis pedis artery and the conduction velocity of peroneal nerve between two groups (P<0.01). At the first, second and third weeks of treatment, there were statistically significant differences in TNF-α, IL-6 and VEGF levels in the serum between two groups (P<0.01). The blood flow velocity of dorsalis pedis artery was positively correlated with TNF-α and IL-6 levels, but was negatively correlated with VEGF level. The conduction velocity of peroneal nerve was positively correlated with VEGF level, but was negatively correlated with TNF-α and IL-6 levels. Conclusion: Qingre Jiedu Huoxue Tongluo formula combined with Fufang Huangbai liquid has a good therapeutic effect on diabetic foot, so it is worthy of clinical popularizing and application.关键词：diabetic foot;Qingre Jiedu Huoxue Tongluo formula;Fufang Huangbai liquid;tumor necrosis factor-α;vascular endothelial growth factor;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of double dose glucosidorum tripterygll totorum combined with ambrette capsule in the treatment of diabetic nephropathy (DN) and observe its effect on the level of inflammatory factors. Method: The 126 patients with DN were divided into control group (with ambrette capsules alone) and observation group (double dose glucosidorum tripterygll totorum combined with ambrette capsules). 8 weeks were regarded as one course of treatment. Before and after the treatment, the inflammatory indexes in serum[interleukin (IL)-18, IL-6, hypersensitive C-reactive protein(Hs-CRP)], liver and kidney function, 24 h urinary protein. Result: The total effective rate in the observation group was significantly higher than that in the control group, with significant difference between two groups(P<0.05). After treatment, urinary protein was significantly decreased, renal function improved, plasma albumin increased, Hs-CRP, IL-18 and IL-6 significantly decreased in both groups (P<0.01). The observation group was significantly better than the control group in the comparison of above indexes (P<0.01). No liver function damage was seen. Conclusion: Double dose glucosidorum tripterygll totorum combined with ambrette capsules can reduce the inflammation indexes in serum, and improve the clinical efficacy of DN.关键词：diabetic nephropathy;glucosidorum tripterygll totorum;ambrette capsules;interleukin(IL)-18;IL-6;hypersensitive c-reactive protein
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发布时间：2024-01-04 - 摘要：Objective: To retrospectively analyze the treatment principles, methods and medication disciplines of Uyghur medicine for limb fractures. Method: Retrospective analysis method was used to collect the medical records of patients with limb fracture from January 2013 to December 2014 in Uyghur Medicine Hospital of Xinjiang Uyghur Autonomous Region, analyze the treatment principles, methods, formulas and drugs for limb fractures, and explore the characteristics and medication disciplines of Uyghur medical treatment methods for fractures. Result: Among 450 fracture cases included in the study, Uyghur medicine manual reduction was used in 284 cases (63.1%), and surgery bonesetting was used in 166 cases (37.9%). The medicines used were mainly of promoting blood circulation, relieving the swelling and inflammation, and promoting the callus formation. These kinds of medicines accounted for 72% of the used medicines. Shalajit Migao, Maddatulhayat Migao Preparations, and Zingiberis Rhizoma, Cinnamomi Cortex, Anakin Root, Piperis Longi Fructus, Semen Alpiniae Katsumadai, Bletillae Rhizoma, Caryophylli Flos, Pericarpium Zanthoxyli, Semen Myristicae, Tsaoko Fructus, Benzoinum, Olibanum, Margarita and other medicinal materials could enhance the body nature, promote the bone metabolism, callus growth and fractures healing. Conclusion: The Uyghur medical treatment methods and medication disciplines for fractures were in conformity with the clinical practice. After manual reduction with plaster external fixation and surgical treatment, corresponding oral medicines shall be taken. The oral medicines include the agents and corresponding medicinal materials which can activate blood circulation to dissipate stasis, relieve swelling and inflammatory, promote bone metabolism, callus growth, and assist in body nature.关键词：limb fractures;clinical records collation;medication disciplines for Uyghur medicine
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Erlong Zuoci Wan combined with Tongqi San in treatment of chronic secretory otitis media (CSOM) with syndrome of deficiency of liver and kidney and observe its effects on aquaporin (AQP)-1 and AQP-4 levels in serum. Method: One hundred and seventeen patients with chronic secretory otitis media in our hospital were randomly divided into control group (59 cases) and treatment group (58 cases) by referring to the random number table method. Patients in the control group were treated with cefetamet pivoxil hydrochloride dispersible tablets (500 mg/time, bid), desloratadine citrate disodium tablets (1 tablet/time, qd) and xylometazoline nasal drops (2 drops/time, bid). Patients in the treatment group received additional Erlong Zuoci Wan combined with Tongqi San (1 dose/d, bid) based on the treatment of control group. The treatment course was 4 weeks for both groups. The traditional Chinese medicine (TCM) symptom scores and bone conduction threshold under different frequencies were compared between two groups. The AQP-1 and AQP-4 levels in serum were detected in two groups. Result: The TCM symptom scores in treatment group were significantly lower than those in the control group after treatment (P<0.01). The total clinical effective rate was 89.61% in the treatment group, higher than 76.92% in the control group (P<0.05). Bone conduction thresholds under different frequencies (1, 2, 4, 8 kHz) in treatment group were significantly lower than those in control one after treatment (P<0.01). The AQP-1 and AQP-4 levels in serum of treatment group were significantly higher than those of control group after treatment, with statistically significant differences (P<0.01). Conclusion: Erlong Zuoci Wan combined with Tongqi San could significantly improve the clinical symptoms of the patients, decrease bone conduction thresholds, and increase the AQP-1 and AQP-4 levels in serum in the treatment of chronic secretory otitis media with syndrome of deficiency of liver and kidney.关键词：Erlong Zuoci Wan;chronic secretory otitis media;syndrome of deficiency of liver and kidney;Tongqi San
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of Tongnao Huoxin decoction in treatment of primary hypertension with syndrome of internal blockade of static blood and observe its effect on blood pressure variability and levels of homocysteine (HCY) and 25-hydroxyvitamin D [25(OH)D] in serum. Method: One hundred and ten patients were randomly divided into control group and treatment group (55 cases in each group) according to random number table in the study. Patients in control group were treated with amlodipine besylate tablets (5 mg/time, 1 tablet/day) and enalapril maleate tablets (10 mg/time, 1 table/day). Patients in treatment group were treated with Tongnao Huoxin decoction orally based on the treatment in control group (1 dose/day, bid). The treatment course was two months in both groups. Changes of hemorheology indexes and blood pressure variability indexes pre-post treatment were compared between two groups. Clinic effects of two groups after treatment were analyzed. Levels of Hcy and 25 (OH) D in serum of two groups were detected. Result: The whole blood high shear viscosity, middle shear viscosity, low shear viscosity and plasma viscosity in treatment group were significantly lower than those of control group after treatment (P<0.01). The total effective rate was 92.73% in treatment group, higher than 76.36% in control group (P<0.05). Standard deviation of diastolic blood pressure in 24 h (24 hDSD), standard deviation of daytime diastolic blood pressure (dDSD), standard deviation of daytime systolic blood pressure (dSSD), and standard deviation of nighttime diastolic blood pressure (nDSD) in treatment group were significantly lower than those of control group after treatment (P<0.01). After treatment, the level of Hcy in serum of treatment group was significantly lower than that of control group, while 25 (OH) D level was significantly higher than that of control group, with statistically significant difference (P<0.01). Conclusion: Tongnao Huoxin decoction based on western medicine could significantly improve blood viscosity, blood pressure variability and raise clinical effects in treatment of primary hypertension with syndrome of internal blockade of static blood, and the mechanism may be associated with reducing the level of Hcy in serum and elevating 25 (OH) D level.关键词：Tongnao Huoxin decoction;primary hypertension;syndrome of internal blockade of static blood;blood pressure variability
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Fufang Changpu Yizhi decoction for mild cognitive impairment (MCI) with phlegm turbidity and stasis syndrome after stroke and investigate its effects on oxidative damage, blood lipid and cerebral blood flow. Method: One hundred and ninety-two eligible patients were randomly divided into control group (96 cases) and observation group (96 cases) by random number table. Patients in the control group received nimodipine tablets by orally taking, 30 mg/time, 1 time/day, patients in the observation group took Fufang Changpu Yizhi decoction orally, 1 dose/day. The treatment course was 12 weeks for both groups. Before and after treatment, cognitive function was evaluated by mini mental state examination (MMSE) and montreal cognitive assessment (MoCA) scales. Scores of phlegm turbidity and stasis, as well as damage of brain collateral by toxin were graded; levels of superoxide dismutase (SOD), malondialdehyde (MDA), homocysteine (Hcy), hemorheology indexes and blood lipid levels were detected. The mean blood flow rates of anterior cerebral artery (ACA) and middle cerebral artery (MCA) were measured once respectively before and after treatment. Result: The total effective rate of cognitive function was 79.17% in observation group, higher than 56.25% in control group (P<0.05). After treatment, scores of MoCA and MMSE in observation group were higher than those in control group, and scores of phlegm turbidity and stasis and damage of brain collateral were lower than those in control group (P<0.01). The levels of total cholesterol(TC), triglyceride(TG) and low density lipoprotein cholesterin(LDL-C) in the observation group were lower than those in control group after treatment (P<0.05), while the level of high density lipoprotein cholesterin(HDL-C) was lower than that in control group (P<0.05). Hemorheological indexes of whole blood viscosity (high shear and low shear), plasma viscosity, fibrinogen and platelet aggregation rate in the observation group were lower than those in control group after treatment (P<0.05, P<0.01). Levels of Hcy and MDA in the observation group were lower than those in control group after treatment, while the level of SOD was higher than that in control group (P<0.05, P<0.01). Mean blood flow rates of ACA and MCA in treatment were higher than those in control group after treatment (P<0.01). Conclusion: Fufang Changpu Yizhi decoction has better effect than nimodipine tablets in improving the cognitive function of patients after stroke and ameliorating clinical symptoms, in addition, it can improve functions of vascular endothelium and blood circulation, regulate lipid metabolism, and promote cerebral hemodynamics.关键词：mild cognitive impairment;Fufang Changpu Yizhi decoction;oxidative damage;homocysteine;blood lipid;hemorheology
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发布时间：2024-01-04 - 摘要：Objective: To analyze Professor ZHANG Pei-qing's medical experience for membranous nephropathy based on Traditional Chinese Medicine Inheritance Support System (V2.5). Method: ZHANG Pei-qing's prescriptions for membranous nephropathy patients were collected and logged into the Traditional Chinese Medicine Inheritance Support System(V2.5). Prescription frequency of drugs, combination of paired herbs and prescription characters were defined by using the software's integrated regularity analysis, improved mutual information, complex system entropy cluster, unsupervised hierarchical clustering and other data-mining methods and through Professor ZHANG Pei-qing's personal proofreading. Result: The 110 prescriptions were screened out and analyzed, involving 93 herbs. After determining the frequency of each herb and the association rules between all herbs, we got 16 core combinations and 8 new prescriptions, and these were confirmed as the core herbs for treating membranous nephropathy by Professor ZHANG Pei-qing. Conclusion: Professor ZHANG Pei-qing's prescriptions show outstanding characteristics in treating membranous nephropathy, with a high accuracy. The analytical results obtained by the Traditional Chinese Medicine Inheritance Support System were basically consistent with the professor's experience, indicating that the system can be used to analyze and summarize clinical experiences of famous veteran doctors of traditional Chinese medicine.关键词：ZHANG Pei-qing;membranous nephropathy;herbal administration experience;Traditional Chinese Medicine Inheritance Support System (V2.5)
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发布时间：2024-01-04 - 摘要：Objective: To assess the efficacy and safety of Shenfu injection in preventing chemotherapy-induced peripheral neurotoxicity in cancer patients. Method: Randomized controlled trials (RCTs) for Shenfu injection in preventing CIPN efficacy were searched in PubMed, Embase, Cochrane Libraries, CNKI, WanFang Data, VIP database since the date of establishment to June 2015. Statistical analysis was performed with RevMan5.3. Result: Nine RCTs involving 1 047 patients were included. According to the results of Meta-analysis. ① The Shenfu group was more effective than the control group with a decreased overall incidence of CIPN [RR=0.53, 95%CI(0.36, 0.77), P=0.000 8]and incidence of severe CIPN [RR=0.48, 95%CI(0.31, 0.74), P=0.000 8]. ② There were no statistical significance in objective response rate between the two groups. The results of subgroup analysis showed that ① The Shenfu group was more effective than the control group with a decreased overall incidence of peripheral neurotoxicity [RR=0.59, 95%CI(0.43, 0.82), P=0.001]and incidence of severe peripheral neurotoxicity induced by oxaliplatin [RR=0.45, 95%CI(0.26, 0.78), P=0.005]. ② Shenfu injection had no effect on CIPN induced by other chemotherapy regimens (excluding oxaliplatin). Conclusion: Shenfu injection shows a significant advantage in preventing oxaliplatin-induced peripheral neurotoxicity (OIPN).关键词：Shenfu Injection;chemotherapy;prevention;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To systematically evaluate the efficacy and safety of Tanreqing injection in treatment of respiratory infections in children. Method: Randomized controlled trials for Tanreqing injection in treatment of respiratory tract infections in children from 2005 to 2015 were searched in VIP, CBM, Wanfang Data, CNKI and Pubmed. In accordance with the inclusion/exclusion standards, efforts were made to select literatures, evaluate the quality of included studies, extract data, and then conduct Meta-analysis by using Revmen 5.3 software. Result: Totally 12 randomized controlled trials were included in the systematic evaluation, all of which were low-quality study with Jadad score of less than 3. The meta results showed that compared with the control group, Tanreqing injection in treatment of children's upper respiratory tract infection can improve clinical total efficiency [OR=6.42, 95% CI (4.32, 9.53), P<0.000 01], shorten the duration of fever [MD=-1.93, 95% CI (-3.22, -0.65), P=0.003], relieve children's throat congestion [OR=12.79, 95% CI (5.23, 31.31), P<0.000 01]and swollen tonsils [OR=2.98, 95% CI (1.43, 6.23), P=0.004], with statistically significant differences; but in terms of shortening the cough length [MD=-1.47, 95% CI (-2.93, -0.01), P=0.05], there was no statistically significant difference from the control group. Conclusion: Tanreqing injection in treatment of respiratory tract infections in children has a better clinical efficacy and high safety. However, the existing studies have a low evidence level, so more large-sample, high-quality, multi-center randomized controlled trials shall be conducted for further verification.关键词：Tanreqing injection;children;upper respiratory infection;randomized control trial;systematic evaluation
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发布时间：2024-01-04 - 摘要：Objective: To study disease location character and disease feature character of pancreas cancer in order to standardize the characteristic features of pancreas cancer. Method: Prospective observation, clustering and principal component analysis on common disease syndromes of pancreas cancer was done by SPSS 18.0 software. Result: Pancreas cancer cases showed that the main disease location of pancreas cancer were spleen, liver, stomach, gallbladder and kidney, taking 100%, 58.6%, 50.7%, 27.3%, 20.5%;main disease features were blood stasis, Qi stasis, Qi deficiency, dampness, phlegm and hot, taking 100%, 74.1%, 66.2%, 59.7%, 38.8%, 34.5%, respectively. In clinic, commonly seen syndrome types include syndrome of damp-heat in liver and gallbladder, syndrome of stagnation of liver qi and spleen deficiency, syndrome of intermin-gled phlegm and blood stasis, syndrome of yin deficiency of liver and kidney, syndrome of spleen deficiency and water retention. Conclusion: Pancreas cancer cases indicates that the main disease location of pancreas cancer were spleen, liver, stomach, gallbladder and kidney and main disease features were blood stasis, Qi stasis, Qi deficiency, dampness, phlegm and hot.关键词：pancreas cancer;disease location character;disease feature character;case clustering;principal component analysis
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发布时间：2024-01-04 - 摘要：It has been a long-term, arduous task for traditional Chinese medicine practitioners to study the "efficacy-enhancing and toxicity-reducing" effect of Chinese herbal medicines. However, they mostly focused on the traditional Chinese herbs with a higher toxicity, such as Tripterygium wilfordii, Aconite Lateralis Radix Praeparata, Aconiti Radix and Aconiti Kusnezoffii, but paid less attention to commonly used herbs with less toxic and side effects. Polygala Radix is commonly used as a nootropic medicine in the clinic, and one of hot herbs in anti-aging and intelligence-reinforcing experiments at present. Polygala Radix has an obvious gastrointestinal side effect, and contains tenuigenin that has an anti-aging and intelligence-reinforcing effect but toxicity. By summarizing the recent advance in studies on common combinations of Polygala Radix, including Polygala Radix-Acori Tatarinowii Rhizoma, Polygala Radix-Magnoliae Officinalis Cortex, Polygala Radix-Mel, Polygala Radix-Ziziphi Spinosae Semen, Polygala Radix-Ginseng Radix et Rhizoma, Polygala Radix-Poria (Fushen), Polygala Radix-Glycyrrhizae Radix et Rhizoma, Polygala Radix-Bistortae Rhizoma, researchers found almost no experimental study combing the intelligence-reinforcing and toxicity-reducing effects. Considering the effectiveness and safety, based on the compatibility, they studied Polygala Radix's "toxicity-reducing and efficacy-enhancing" effects in the expectation of obtaining valuable evidence from laboratory, which is of great significance to guiding clinical medication of Polygala Radix and developing new drugs.关键词：Polygala Radix;compatibility;experimental study;toxicity-reducing and efficacy-enhancing
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发布时间：2024-01-04 - 摘要：In clinical, allergic rhinitis and asthma often exist simultaneously. The World Allergy Organization (WAO) has formally proposed the new medical diagnostic term combined allergic rhinitis and asthma syndrome(CARAS), which is considered as a whole to treat the upper and lower respiratory tract diseases for combined diagnosis and combined treatment. CARAS is mainly manifested with simultaneous occurrences of clinical or sub clinical symptoms of chronic inflammation in the upper and lower respiratory tract and highly reactive symptoms, including nasal itching, sneezing, runny nose, nasal congestion, cough and wheezing. These symptoms can suddenly attack, but also can relieve spontaneously or quickly disappear after treatment. There is no corresponding name of CARAS in traditional Chinese medicine (TCM). Allergic rhinitis and asthma can be respectively classified into the category of "sniveling nose" and "asthma syndrome" in TCM. In recent years, the TCM research on CARAS has gradually increased, but it is still in the initial stage and has not yet formed a complete syndrome differentiation and treatment system. This paper has summarized the research status of adult CARAS in western medicine and reviewed the research progress of TCM treatment from the pathogenesis, syndrome distribution, clinical treatment, and TCM mechanism of action, indicating that rich experience has been accumulated in TCM treatment, with certain advantages in clinical efficacy, but there is still a lack of systemic research on CARAS. The paper has also put forward the problems of TCM treatment in the syndrome research, clinical research and basic research, which would provide new research ideas for the TCM treatment of adult CARAS.关键词：combined allergic rhinitis and asthma syndrome in adults;traditional Chinese medicine(TCM);pathogenesis;syndrome distribution;clinical treatment;TCM mechanism
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