最新刊期
      卷 22 ， 期 13 ， 2016
      • LI Chun-qin,WANG Zi-yan,CHEN Lu,LIU Mei-lin,ZHANG Mei
        Vol. 22, Issue 13, Pages: 1-6(2016) DOI: 10.13422/j.cnki.syfjx.2016130001
        摘要：Objective: To investigate the relationship between HPLC fingerprints and hepatoprotective activity of ethyl acetate extract from Herpetospermum caudigerum. Method: Fingerprints of different combination of ethyl acetate extract from H. caudigerum were established by HPLC and were evaluated by hierarchical clustering analysis. In addition,nine samples of ethyl acetate fraction from H. caudigerum with different chemical composition were acquired by uniform design and silica gel column chromatography.The hepatoprotective activity of H. caudigerum were evaluated with models of immunologic liver injury in mice,and the models were induced by injecting bacille calmette-guerin (BCG) and lipopolysaccharide (LPS) through the tail vein.The spectrum-effect relationship between HPLC fingerprints and hepatoprotective activity was investigated by grey relational analysis and partial least squares regression analysis (PLSR). Result: There were significantly differences in HPLC fingerprints of 9 samples,and 16 common characteristic peaks were detected in HPLC chromatography.Compared with the model group,the treatment with 9 samples restored the serum levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST) and decreased the content of malonaldehyde (MDA) in BCG/LPS-induced immunological liver injury mice.The spectrum-efficacy relationship found that the main anti-immunological liver injury effect was mostly due to herpetin,herpetotriol,herpetrione,herpetone and herpetal. Conclusion: Each sample exhibits good activity on anti-liver injury,and that some lignan constituents, such as herpetin,herpetrione,herpetotriol,herpetone and herpetal, might be the main contributive components in H. caudigerum to hepatoprotective activity.  
        关键词：Herpetospermum caudigerum;ethyl acetate extract;fingerprints;immunological liver injury;grey relational analysis;partial least squares regression analysis;spectrum-effect relationship   
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        发布时间：2024-01-04
      • ZANG Qiao-zhen,TANG Tao,LONG Kai-hua,WANG Chun-liu,LI Ye
        Vol. 22, Issue 13, Pages: 7-10(2016) DOI: 10.13422/j.cnki.syfjx.2016130007
        摘要：Objective: To optimize formulation of thermo-sensitive in situ nasal gel of α-asarone nanoparticles and investigate its in vitro release behavior. Method: With amounts of poloxamer 407 and poloxamer 188 as independent variables,gelation temperature as an dependent variable,multilinear and quadratic models were used to estimate the relationship between dependent variable and independent variables and to select the formulation by central composite design-response surface methodology.Artificial simulated nasal fluid was applied as release medium to study in vitro release characteristics of α-asarone nanoparticles in situ gel. Result: Optimal formulation was as follows:poloxamer 407 of 21.85% and poloxamer 188 of 3.8%,gelation temperature was (33.7±0.1)℃.Within 72 hours,accumulative release of α-asarone was 70.42%. Conclusion: α-asarone nanoparticles in situ gel possesses a superior sustained-release effect,the optimum formulation provides a basis for development of new administration routes and dosage forms of α-asarone.  
        关键词：α-asarone;nanoparticles;in situ gel;in vitro release;poloxamer   
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        发布时间：2024-01-04
      • YU Di-jia,LIU Yang,ZHU Ying
        Vol. 22, Issue 13, Pages: 11-14(2016) DOI: 10.13422/j.cnki.syfjx.2016130011
        摘要：Objective: To optimize the formulation of osthole solid dispersible tablets and investigate its in vitro release characteristics. Method: Taking composite score of disintegration and hardness as index,formulation of osthole solid dispersible tablets was optimized by orthogonal test.Release characteristics of osthole solid dispersible tablets and osthole were investigated by RP-HPLC. Result: The proportion of each adjuvant in optimal formulation was 40% of microcrystalline cellulose (MCC),4% of low-substituted hydroxypropyl cellulose (L-HPC),12% of crosslinked polyvidone (PVPP),43.5% of osthole solid dispersion and 0.5% of aerosil.The tablets disintegrated in 80.5 s,the hardness was 4.4 kg,the dispersible uniform was excellent.Release parameters of osthole solid dispersible tablets were remarkly better than these of osthole group;tmax of osthole solid dispersible tablets group was1 h,its Cmax was 37.42 mg·L-1,AUC0-t was 43.04 mg·L-1·h. Conclusion: Osthole solid dispersible tablets has characteristics of uniformly dispersing and quick releasing of active constituents,it can significantly inprove the bioavailability of osthole in rats.  
        关键词：osthole;solid dispersion;dispersible tablets;disintegration;dissolution;pharmacokinetics   
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        发布时间：2024-01-04
      • HU Jun-hua,WANG Xiao-jiao,QIAO Shan-yi,WU Yun,HUANG Wen-zhe,WANG Zhen-zhong
        Vol. 22, Issue 13, Pages: 15-18(2016) DOI: 10.13422/j.cnki.syfjx.2016130015
        摘要：Objective: To establish a method for determining in vitro dissolution of three components in Liuwei Dihuang Gantang tablets. Method: HPLC was adopted to determine contents of morroniside,loganin and paeoniflorin with the mobile phase of methanol-acetonitrile-water for gradient elution and the detection wavelength at 236 nm.Taking water as dissolution medium,cup method was employed to determine in vitro dissolution of these three components. Result: The linear ranges of morroniside,loganin and paeoniflorin were 4.87-155.75,4.86-155.35,2.83-180.85 mg·L-1 with average recoveries of 99.88%(RSD 1.6%),101.10%(RSD 1.8%) and 100.20%(RSD 1.4%),respectively.The accumulation dissolution of each component in 90 minutes was more than 75%. Conclusion: Differences of the accumulation dissolution of morroniside,loganin and paeoniflorin in identical batch and different batches of samples are small.This established method is repeatable for quality control of Liuwei Dihuang Gantang tablets.  
        关键词：Liuwei Dihuang Gantang tablets;dissolution;morroniside;loganin;paeoniflorin   
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        发布时间：2024-01-04
      • ZHAO Li-jian,LIU Shan-xin,SU Ming,BI Jian-yun,WANG Ping,FENG Cheng
        Vol. 22, Issue 13, Pages: 19-22(2016) DOI: 10.13422/j.cnki.syfjx.2016130019
        摘要：Objective: To optimize preparation process of Huoxue cataplasm. Method: Taking composite score of adhesion,appearance uniformity,film residue,skin compliance,spreadability and others as index,based on single factor tests,central composite design-response surface methodology was used to optimize preparation process with amounts of sodium polyacrylate-700,glycerin,aluminium glycinate and povidone K-90 as factors. Result: Optimum substrate composition was sodium polyacrylate-700/polyvinyl/glycerin/aluminium glycinate/povidone K-90/aerosil/water/ointment was 4.7:0.5:29.2:0.5:3:4:22.4:12.9.Cataplasm was coated easily with smooth cream surface,consistent color,good skin followability and no leakage. Conclusion: This preparation process is rational and suitable for production of Huoxue cataplasm.  
        关键词：Huoxue cataplasm;matrix prescription;molding process;sodium polyacrylate-700;aluminium glycinate;aerosil   
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        发布时间：2024-01-04
      • LU Shi-hui,LIN Yao,CHEN Ran,LU Hong-mei
        Vol. 22, Issue 13, Pages: 23-26(2016) DOI: 10.13422/j.cnki.syfjx.2016130023
        摘要：Objective: To optimize technological conditions of ultrasonic wave-enzymes extraction for chelerythrine and its 6-alkoxy derivatives from Zanthoxyli Radix. Method: HPLC was employed to determine contents of chelerythrine and its 6-alkoxy derivatives with mobile phase of acetonitrile-water-phosphoric acid-triethylamine (25:75:0.2:0.25) and detection wavelength at 284 nm.Orthogonal test and single factor tests were adopted to optimize extraction process of chelerythrine and its 6-alkoxy derivatives from Zanthoxyli Radix. Result: The optimal process was as follows:pretreated Zanthoxyli Radix powder with cellulase (1:250) and pectinase (1:250) for 30 min in 3.7 times volume of acetic acid-sodium acetate buffer (pH 5) at ambient temperature (≥30℃),and then extracted 3 times by ultrasonic-wave (250 W) with 40%ethanol (containing 0.5%hydrochloric acid) as solvent,extracted 18 min with 6 times solvent at the first time,extracted 15 min with 3 times solvent at the second time,extracted 12 min with 3 times solvent at the third time.The extraction rate of chelerythrine and its 6-alkoxy derivatives was 90.5%. Conclusion: This optimized process can improve economical benefit and efficiency,save energy and time,which provides experimental base for industrial production of chelerythrine from Zanthoxyli Radix.  
        关键词：Zanthoxyli Radix;chelerythrine;ethoxy chelerythrine;ultrasonic method;enzymic method   
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        发布时间：2024-01-04
      • ZHANG Run-rong,YE Xue-feng,GAO Jin,CHEN Hai-yan
        Vol. 22, Issue 13, Pages: 27-30(2016) DOI: 10.13422/j.cnki.syfjx.2016130027
        摘要：Objective: To optimize molding process of Fengtongling plaster and develop GC for assay of methyl salicylate in this preparation. Method: Orthogonal test was adopted to optimize molding technology of Fengtongling plaster with melting point,gloss,hardness and smooth as indexes.The content of methyl salicylate was determined by GC,chromatographic conditions was PEG-20M column (0.25 mm×30 m,0.25 μm),split ratio was 50:1. Result: The best proportion of paraffin wax,beeswax,white petrolatum and lanolin were 4%,6%,5% and 3%,respectively,liquid temperature was 65-75℃.Three batches of products conformed standards of the 2010 edition of Chinese Pharmacopoeia on ointments.The linear range of methyl salicylate was 0.66-13.25 g·L-1,average recovery was 98.94% with RSD of 0.7%. Conclusion: This optimized molding process is feasible and stable,this determination method is simple,rapid and accurate for quality control of Fengtongling plaster.  
        关键词：Fengtongling plaster;preparation process;methyl salicylate;capillary GC;paraffin wax;beeswax;lanolin   
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        发布时间：2024-01-04
      • LI Xi-xiang,LI Xing-yong,LIU Xiao-shuan,WANG Xue-mei,BAO Qiang
        Vol. 22, Issue 13, Pages: 31-33(2016) DOI: 10.13422/j.cnki.syfjx.2016130031
        摘要：Objective: To optimize percolation technology of Gubi Zhitong liquor and to provide a reference for industrial production of this preparation. Method: Taking composite score of extracting amounts of imperatorin,ferulic acid,cinnamaldehyde and dry extract yield as index,orthogonal test was adopted to optimize percolation technology with powder fineness,soaking time,percolation speed and liquor amount.HPLC was employed to determine contents of imperatorin,ferulic acid and cinnamaldehyde with detection wavelengths of 254,320,290 nm and mobile phases of methanol-water (60:40),methanol-1% glacial acetic acid solution (30:70) and methanol-water (35:65). Result: Optimal percolation technology was as follows:powder fineness of 50 mesh,soaked 24 h with 10 times the amount of liquor,percolation speed of 2 mL·min-1.Under these conditions,extracting amounts of imperatorin,ferulic acid,cinnamaldehyde were 69.888,171.840,123.845 μg·g-1, dry extract yield was 32.16%. Conclusion: This optimized percolation process is simple and stable with good repeatability,it can provide a theoretical basis for further developing of Gubi Zhitong liquor.  
        关键词：Gubi Zhitong liquor;percolation process;composite score;Imperatorin;ferulic acid;cinnamaldehyde   
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        发布时间：2024-01-04
      • ZHAI Hong-wei,PAN Xiao-juan,SHU Guang-ming,ZHOU Xian-jian,YANG Xin-xin,ZHANG Mei
        Vol. 22, Issue 13, Pages: 34-39(2016) DOI: 10.13422/j.cnki.syfjx.2016130034
        摘要：Objective: To optimize percolation technology of Gubi Zhitong liquor and to provide a reference for industrial production of this preparation. Method: Taking composite score of extracting amounts of imperatorin,ferulic acid,cinnamaldehyde and dry extract yield as index,orthogonal test was adopted to optimize percolation technology with powder fineness,soaking time,percolation speed and liquor amount.HPLC was employed to determine contents of imperatorin,ferulic acid and cinnamaldehyde with detection wavelengths of 254,320,290 nm and mobile phases of methanol-water (60:40),methanol-1% glacial acetic acid solution (30:70) and methanol-water (35:65). Result: Optimal percolation technology was as follows:powder fineness of 50 mesh,soaked 24 h with 10 times the amount of liquor,percolation speed of 2 mL·min-1.Under these conditions,extracting amounts of imperatorin,ferulic acid,cinnamaldehyde were 69.888,171.840,123.845 μg·g-1, dry extract yield was 32.16%. Conclusion: This optimized percolation process is simple and stable with good repeatability,it can provide a theoretical basis for further developing of Gubi Zhitong liquor.  
          
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        发布时间：2024-01-04
      • ZHANG Cai-hong,MA Yu-ying,GU Rui,ZHONG Shi-hong,HU Ming-xun,WANG Na
        Vol. 22, Issue 13, Pages: 40-43(2016) DOI: 10.13422/j.cnki.syfjx.2016130040
        摘要：Objective: To study the morphology and histology of Tibetan medicine Gentiana veitchiorum and determine the main identification characteristics. Method: Original plants and herbal properties were identified with pharmacognostical method.Its identification characteristics were described in this paper.The slide made with chloral hydrate and epidermis flaking methods was adopted to observe the microscopic identification of the powder and leaves.By using microscope, the graphs of herbal powders were printed, and parts of the microscopic characters were photographed.The pollen grains and seeds were naturally dried, stuck on test-bed and gold plated, then their micromorphological features were observed by scanning electron microscopy (SEM). Result: The identification characteristics of G.veitchiorum were determined in terms of morphology and histology. Conclusion: The study offers a scientific basis for accurate identification and application of Tibetan medicine G.veitchiorum.  
        关键词：Gentiana veitchiorum;original plant;herbal property;microscopic identification;micromorphological feature   
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        发布时间：2024-01-04
      • ZHANG Peng-ge,AN Lu-sha,SHENG Ping,REN Hui-mei
        Vol. 22, Issue 13, Pages: 44-49(2016) DOI: 10.13422/j.cnki.syfjx.2016130044
        摘要：Objective: To compare the contents of total alkaloids, sipeimine, sipeimine-3β-D-glucoside, adenosine and thymidine in Fritillariae Pallidiflorae Bulbus from different producing areas in Xinjiang, in order to identify the optimal growth area by principal component analysis and cluster analysis. Method: The five ingredients in Fritillariae Pallidiflorae Bulbus from different producing areas were measured by acid dye colorimetric method, high performance liquid chromatography(HPLC) and ultra performance liquid chromatography-evaporative light scattering detector(UPLC-ELSD). Data analysis was performed by SPSS 19.0. Result: The contents in Fritillariae Pallidiflorae Bulbus varied greatly in different producing areas, but similar in the same producing area. The scores and ranks of principal components were higher in Jimusaer County, Mulei County, Qitai County, Guozigou of Huocheng County, Sayram Lake of Yili and Wenquan County in Xinjiang. The scores and ranks of principal components were lower in Xinyuan County. Conclusion: Jimusaer County, Mulei County, Qitai County, Guozigou of Huocheng County, Sayram Lake of Yili and Wenquan County are the optimal producing areas of Fritillariae Pallidiflorae Bulbus. Meanwhile, temperature, moisture, light and soil in different producing areas have impacts on the content of ingredient, and so shall be taken into full account in selecting producing areas of Fritillariae Pallidiflorae Bulbus.  
          
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        发布时间：2024-01-04
      • YIN Yan,DANG Bo-yue,LIU Shi-hui,ZHAO De-gang
        Vol. 22, Issue 13, Pages: 50-54(2016) DOI: 10.13422/j.cnki.syfjx.2016130050
        摘要：Objective: To analyze the differences on photosynthetic characteristics between tetraploid and diploid Polygonum capitatum. Method: LI-6400XT portable photosynthesis system was used to measure various photosynthetic physiological parameters of tetraploid and diploid P. capitatum and their variation regularities. Result: The diurnal variation of net photosynthetic rate (Pn) of tetraploid and diploid P.capitatum s leaves showed double-peak curves with the phenomenon of ‘midday depression’, but the Pn of tetraploid was higher than that of diploid.Both P.capitatum cultivars' diurnal variation of stomatal conduction (Gs) and transpiration rate (Tr) were consistent with that of Pn, and intercellular CO2 concentration (Ci) diurnal variation trend were exactly opposite to that of Pn.Gs, Tr, and water use efficiency (WUE) of tetraploid were higher than that of diploid, but Ci of tetraploid was lower than that of diploid.Light compensation point (LCP) of diploid P.capitatum [67.83 μmol·(m2·s)-1] was higher than that of tetraploid P.capitatum [41.43 μmol·(m2·s)-1], and light saturation point (LSP) of diploid P.capitatum [1225 μmol·(m2·s)-1] was lower than that of tetraploid P.capitatum [1370 μmol·(m2·s)-1].The maximum net photosynthetic rate (Pnmax) of diploid P.capitatum [5.36 μmol·(m2·s)-1] was lower than that of tetraploid P.capitatum [8.83 μmol·(m2·s)-1], and apparent quantum efficiency (AQY) of diploid P.capitatum [0.01 μmolphotons·(m2·s)-1] was lower than that of tetraploid P.capitatum[0.02 μmolphotons·(m2·s)-1]. Conclusion: Photosynthetic performance of tetraploid P.capitatum was superior to that of diploid P.capitatum.  
        关键词：Polygonum capitatum;tetraploid;photosynthetic characteristics;stomatal conduction;photosynthetic rate;transpiration rate   
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        发布时间：2024-01-04
      • HU Pan,XIA Hou-lin,LI Rui-yu,WEN Bing-jie,YANG Meng-ni,ZHANG Hui
        Vol. 22, Issue 13, Pages: 55-58(2016) DOI: 10.13422/j.cnki.syfjx.2016130055
        摘要：Objective: To isolate balsamic acid esters from benzoin, identify the structures and preliminarily study their efficacy. Method: Benzoin was extracted by 95% ethanol, further separated and purified by column chromatography; the isolated compounds were identified by1H-NMR and 13C-NMR data. The efficacy of these isolated compounds was studied. Result: Isolated compound from benzoin was identified as coniferyl benzoate. Pharmacological experiments showed that the coniferyl benzoate and benzoin group exhibited similar effects, both of which significantly increased evans blue content in the brains of normal mice and exhibited protective effect on endotoxin fatal attack in D-galactosamine-sensitized mice. Conclusion: Coniferyl benzoate is an important component in total balsam acid esters of benzoin and has significant correlation with efficacy of crude benzoin.  
        关键词：coniferyl benzoate;benzoin;total balsamic acids;total balsamic acid esters;blood-brain barrier;endotoxin   
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        发布时间：2024-01-04
      • LIU Bin,LIU Guo-liang,LI Yan-wei,MU Lu-lu,FANG Heng,ZHANG Ning,LIU Hai-yang
        Vol. 22, Issue 13, Pages: 59-62(2016) DOI: 10.13422/j.cnki.syfjx.2016130059
        摘要：Objective: To establish GC-MS fingerprint of Clerodendranthus spicatus volatile components. Method: Volatile components in twelve batches of C. spicatus samples were analyzed by GC-MS method combined with temperature-programmed retention index technique. Research was performed on DB-5MS chromatographic column; carrier gas was helium (He),its flow rate was 1.0 mL·min-1, and the injection volume was 1 μL, the refractive ratio of distillation was 100:1.MS conditions were as follows:electron impact (EI) ion source, transmission line temperature of 280℃, ion source temperature of 230℃, quadrupole temperature of 150℃, and mass scan range of m/z 30-600. Result: Thirteen common peaks were identified in the GC-MS fingerprint of volatile components from 12 batches of C. spicatus samples. Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica(Version 2004 A) was used for fingerprint analysis. Similarity of 12 batches of C. spicatus samples was higher than 0.999. Conclusion: GC-MS combined with temperature-programmed retention index can more comprehensively reflect the volatile components of C.spicatus. The established GC-MS fingerprint is highly characteristic and specific, which can be used as an effective method for quality control of volatile components of C.spicatus.  
        关键词：Clerodendranthus spicatus;GC-MS;volatile components;retention index;fingerprint   
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        发布时间：2024-01-04
      • LU Jun-yu,CHENG Hong-ping,HU Yuan,LIU Jia-yu,LIANG Yi-chuan,LIU You-ping,CHEN Lin
        Vol. 22, Issue 13, Pages: 63-67(2016) DOI: 10.13422/j.cnki.syfjx.2016130063
        摘要：Objective: To study the changes of the water content, superoxide dismutase(SOD), peroxidase(POD) and lipid oxidation end-products of malondialdehyde(MDA) during the storage of Lycii Fructus, to study the mechanism of ‘Oil-spilling’, and establish its scientific storage methods. Method: Two storge methods, including temperature at 40℃, humidity of 75%,temperature at 4℃, humidity of 60%,respectively. Observed the appearance of Lycii Fructus at 0, 20, 40, 60 days. While measured polysaccharide and water by Chinese Pharmacopoeia 2010 edition under the medlar method. MDA was tested by TBA method, SOD activity was measured by nitroblue tetrazolium reduction blue, POD was measured by nordihydroguaiaretic phenol method. Result: ‘Oil-spilling’ was started from 20 day at the storage of 40℃, humidity of 75%, inhibited time-dependent, while the moisture and MDA content were increased, SOD and POD activities were decreased. At the same time, there's no significantly changes under the storage of 4℃, humidity of 60%. Conclusion: Imoroer storage and the water content and reactive oxide species of wolfberry increased. Result in the cell structure and function damaged, cell membrane lipid peroxidated, or permeability changed.  
        关键词：Lycii Fructus;water content;malondialdehyde;peroxidase;superoxide dismutase;Oil-spilling   
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        发布时间：2024-01-04
      • LI Yun,WANG Xiao-li,SONG Jia-ling,QI Huan-yang
        Vol. 22, Issue 13, Pages: 68-70(2016) DOI: 10.13422/j.cnki.syfjx.2016130068
        摘要：Objective: To investigate the chemical components from leaves of Gardenia jasminoides, in order to provide a scientific basis for its development and utilization. Method: The compounds were isolated and purified from 95%ethyl acetate fraction and water extract of leaves of Gardeniae Fructus that were collected from Lishui Country, Nanjing. Separation techniques, such as column chromatography over Sephadex LH-20, macroporous resin and C18, were adopted. Structures were identified through spectroscopic techniques(1H-NMR,13 C-NMR) and physicochemical properties. Result: Eight compounds were isolated and identified as β-gardiol (1), α-gardiol (2), β-sitosterol (3), kaempferol (4), quercetin (5), geniposide (6), gardenoside (7) and β-daucosterol(8). Conclusion: Compounds 1, 2, 4-7 are isolated from leaves of G. jasminoidesfor the first time.  
          
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        发布时间：2024-01-04
      • ZHANG Jing,ZHOU Nong,DING Bo,ZHANG Ya-nan,CHEN Xiu-hong,QI Jun-sheng
        Vol. 22, Issue 13, Pages: 71-76(2016) DOI: 10.13422/j.cnki.syfjx.2016130071
        摘要：Objective: To establish a HPLC method for simultaneously determining the contents of three nucleosides, namely uridine, guanosine and adenosine, and compare the content differences of the three nucleosides and the composition ratios in Corydalis Rhizoma from different regions and commercial grades. Method: A Venusil MP C18 (2) chromatographic column (4.6 mm×250 mm, 5 μm) was used, with methanol (A)-water (B) as the mobile phase for gradient elution (0-10 min, 1%-5%A; 10-15 min, 5%-15%A; 15-22 min, 15%-17%A; 22-26 min, 17%-20%A; 26-32 min, 20%-24%A), at a flow rate of 1.0 mL·min-1. UV detection wavelength was set at 260 nm, and the temperature of the column was maintained at 35℃. The above HPLC method was applied to determine 38 batches of Corydalis Rhizoma and a cluster analysis was carried out for the results. Result: The contents of three nucleosides in Corydalis Rhizoma were determined, and showed a good linear relationship within the linear range (r > 0.999 8). The average recovery rate was in the range of 99.63%-104.24%, with the RSD raging from 0.7%to 2.3%. The three nucleosides could be detected in all 38 batches of Corydalis Rhizoma. There were significant differences in contents of uridine, guanosine and adenosine and the composition ratios for Corydalis Rhizoma from different regions; there were significant differences in contents of the three nucleosides and composition ratios for Corydalis Rhizoma from different farmer households of a same region. 38 batches of Corydalis Rhizoma were divided into three categories by cluster analysis. Conclusion: Overall, the contents of three nucleosides in Corydalis Rhizoma showed significant difference while there was no significant difference in nucleosides content for the Corydalis Rhizoma with different commercial grades.  
        关键词：Corydalis Rhizoma;different regions;commercial grades;uridine;guanosine;adenosine;HPLC method;cluster analysis   
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        发布时间：2024-01-04
      • CUI Sheng-fei,QI Guang-cai,XIE Fan-fan,WU Jiang-rui,LIU Zhen-ye
        Vol. 22, Issue 13, Pages: 77-80(2016) DOI: 10.13422/j.cnki.syfjx.2016130077
        摘要：Objective: To establish a HPLC method for simultaneously determining the contents of chlorogenic acid,gardenoside,paeoniflorin and liquiritin in Lingyang Qingfei powder. Method: A double wavelength HPLC method was performed on Kromasil C18 column (4.6 mm×250 mm,5 μm) with acetonitrile and 0.1% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was set at 1.0 mL·min-1 and the column temperature was 28℃. The detector wavelength was 327 nm for chlorogenic acid and 237 nm for gardenoside,paeoniflorin and liquiritin. Result: The chlorogenic acid,gardenoside,paeoniflorin and liquiritin showed good linear relationship with peak area at the sample size of 1.579-78.96 μg(r=0.9998),1.261-63.04 μg(r=1),0.364-18.2 μg(r=0.9999),and 0.329 6-16.48 μg(r=0.9999) respectively. The average recovery rate was 97.6%,97.6%,97.1% and 99.8% respectively for the above components,with RSD of 1.0%,0.7%,1.2% and 1.3%,respectively. Conclusion: The method is simple,rapid and accurate,and can be used for the quality control of Lingyang Qingfei powder.  
          
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        发布时间：2024-01-04
      • SUN Hai-tao,HE Song-qi,WEN Bin,FAN Er-yan,CHENG Guan-xin,SUN Jia-ling,AN Hai-yan,PANG Jie
        Vol. 22, Issue 13, Pages: 81-85(2016) DOI: 10.13422/j.cnki.syfjx.2016130081
        摘要：Objective: To discuss the molecular mechanism of Biejiajian Wan in inhibiting hepatocellular carcinoma by studying its effect on the signaling molecules of Wnt/β-catenin signaling pathway and the expression of target genes on HepG2 cell xenograft in nude mice. Method: The hepatocellular carcinoma mode was established by tumor formation experiment in nude mice, and the mice were randomly divided into the high dose group (2.2 g·kg-1), the middle dose group (1.1 g·kg-1), the low dose group (0.55 g·kg-1), the positive control group, and the normal control group. According to the visual observation, Biejiajian Wan had an inhibitory effect on the growth of implanted tumors of HepG2 cell xenograft in nude mice. Expressions of β-catenin and T-box 3 (TBX3) of HepG2 cell xenograft were measured by the immunohistochemical method. Expressions of p19ARF, murine double minute 2 (MDM2) and p53 in HepG2 cell xenograft were tested by Western blot. Result: Biejiajian Wan can significantly inhibit the growth of the implanted tumor of HepG2 cell xenograft in nude mice, and the expressions of β-catenin and TBX3 (P < 0.05), as well as the expression of the p19ARF, p53 and MDM2 (P < 0.05). Conclusion: Biejiajian Wan can regulate the expression of β-catenin and TBX3 by affecting the Wnt/β-catenin signaling pathway, and then activate the cancer suppressor gene p53 by regulating the TBX3/p19ARF/MDM2 signaling pathway. Finally, Biejiajian Wan can repress the growth of liver cancer cells and induce cell apoptosis, so as to resist hepatocellular carcinoma.  
        关键词：Biejiajian Wan;xenograft;β-catenin;T-box 3;p19ARF;murine double minute 2;p53   
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        发布时间：2024-01-04
      • FANG Zhu-yuan,DING Kang,SHEN Zhu-yang,JIANG Wei-min
        Vol. 22, Issue 13, Pages: 86-91(2016) DOI: 10.13422/j.cnki.syfjx.2016130086
        摘要：Objective: To investigate the effects of Qianyang Yuyin granules (QYYYG) on oxidative stress and inflammation factors in kidney of spontaneously hypertensive rats (SHRs). Method: SHRs were randomly divided into model group,QYYYG group,Benazepril group,and QYYYG+Benazepril group. The rats received QYYYG suspension (5 g·kg-1),Benazepril suspension (1.67 mg·kg-1),QYYYG suspension (5 g·kg-1)+Benazepril suspension (1.67 mg·kg-1) respectively by intragastric administration. WKY rats were used as normal group,and their renal tissues were collected after 8 weeks of same volume administration.The changes of blood pressure,trace albumin,and creatinine in rats were observed;local NADPH oxidase-4 (NOX4) levels,nuclear factor-κB (NF-κB) p65,interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) protein expression levels of kidney were detected by Western blot;the effects of QYYYG on the release of these indicators were observed. Result: Compared with the normal group,the model gorup showed significant increase in blood pressure, trace albumin, and creatinine in rats, and NOX4,NF-κB p65, IL-6, and TNF-α protein expression levels in kidney of SHRs(P < 0.01). QYYYG can inhibit the expression of NOX4,reduce the NF-κB p65,IL-6, and TNF-α protein expression levels in kidney of SHRs,meanwhile it can reduce blood pressure,trace ablumin, and creatintine(P < 0.01),and the synergy effect was more obvious in case of combination of Qianyang Yuyin granules and Benazepril. Conclusion: QYYYG can improve the renal impairment of SHRs,and the mechanism may be associated with inhibiting oxidative stress and reducing the release of inflammation factors.  
        关键词：Qianyang Yuyin granules;hypertensive renal damage;oxidative stress;nuclear factor-κB;spontaneously hypertensive rats   
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        发布时间：2024-01-04
      • LI Xiao-dong,LI Cang-hai,LI Lan-fang,GUO Shu-ying,JIANG Ting-liang
        Vol. 22, Issue 13, Pages: 92-96(2016) DOI: 10.13422/j.cnki.syfjx.2016130092
        摘要：Objective: To study the effects and possible mechanisms of phenylallyl compounds from Guizhi Tang (PCGZT) on learning and memory impairment in APP transgenic Alzheimer's disease(AD) mice. Method: Three-month-old APP transgenic AD mice were randomly divided into model group, aspirin group, piracetam group, huperzine-A group, PCGZT groups (high, medium and low doses), and normal control group (C57BL/6J mice) according to their weight, with 10 in each group. These mice were intragastrically administered with aspirin (20 mg·kg-1·d-1), piracetam (600 mg·kg-1·d-1), huperzine-A (0.3 mg·kg-1·d-1) and different doses of PCGZT (64.4, 32.2, 16.1 mg·kg-1·d-1) respectively for consecutively 5 months. Model groups and normal control group were treated with 0.5%carboxymethy cellulose sodium (CMC-Na). The abilities of learning and memory of mice were detected by step down test and morris water maze test. The contents of matrix metalloprotein-2 (MMP-2) and matrix metalloprotein-9 (MMP-9) in the blood serum and malondialdehyde (MDA) activity in brain tissues were measured. Result: Compared with model group, PCGZT can reduce latent reaction time (P < 0.05), error frequency (P < 0.01) and total time of error period (P < 0.01), improve total time of safety period (P < 0.05) of APP transgenic AD mice in step down test, low dose group of PCGZT can improve percentage of the platform quadrant distance (P < 0.01) and the platform quadrant time (P < 0.05) in morris water maze test. At the same time, PCGZT can decrease MDA and MMP-2 contents in the serum (P < 0.05). Conclusion: PCGZT can dramatically alleviate the learning and memory impairment of APP transgenic AD mice, and its mechanism may involve multiple drug targets.  
          
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        发布时间：2024-01-04
      • LIANG Wei-hai,HUANG Peng,LI Lun,ZHOU Zhen-ting,CHEN Yu-yao,LIU Qiang,LYU Zhi-ping
        Vol. 22, Issue 13, Pages: 97-101(2016) DOI: 10.13422/j.cnki.syfjx.2016130097
        摘要：Objective: To study and explore the effect and mechanism of Kuan Quzhen gelatum on rat model with eczema. Method: The eczema model of rat was established with 2,4-dinitrochlorobenzene. Totally 40 rats were divided into four groups, namely normal group, eczema group, positive control group and Kuan Quzhen gelatum group. The effect was observed by skin lesion score. Pathological changes were observed by paraffin sections. Interferon-γ(IFN-γ), interleukin-4(IL-4), interleukin-17 (IL-17), interleukin-31 (IL-31), leukotriene B4 (LTB4) and leukotriene C4 (LTC4) were measured by enzyme-linked immunosorbent assay. The expressions of cysteinyl leukotriene receptor 1 (CysLTR1) and cysteinyl leukotriene receptor 2 (CysLTR2) were detected by immunohistochemistry method and Western blot. Result: Compared with normal group, eczema model group showed hyperkeratosis, parakeratosis, hypergranulosis and acanthosis, with increases in IL-4, IL-17, IL-31, LTB4 and LTC4 and up-regulation in the expressions of CysLTR1 and CysLTR2.Compared with eczema model group, Kuan Quzhen Gelatum group showed reductions in skin lesion score after 6 days, pathological changes, and the level of IL-4, IL-17, IL-31 and LTC4 (P < 0.05), and down-regulations in the expressions of CysLTR1 and CysLTR2, with no statistically significant difference in the level of LTB4. Conclusion: Kuan Quzhen Gelatum has therapeutic effects on rat model with eczema, which may be related with declines in IL-4, IL-17, IL-31 and LTC4 levels in serum and down-regulation in CysLTR1 and CysLTR2 expressions.  
        关键词：Kuan Quzhen gelatum;eczema;inflammatory factor;cysteinyl leukotriene receptor   
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        发布时间：2024-01-04
      • XU Zheng-xu,ZHU Shi-guo,LUO Jun,PAN Nian-song
        Vol. 22, Issue 13, Pages: 102-106(2016) DOI: 10.13422/j.cnki.syfjx.2016130102
        摘要：Objective: To study the effect of Guizhou Zedoary Turmeric Oil on cell proliferation of human rectal cancer cells SW1463, in order to detect the effect of expressions of immune factor interleukin(IL)-2 and transforming growth factor-β1 (TGF-β1), and the effect of FasL, Capase-3 and matrix metalloproteinase-9 (MMP-9) protein expressions. Method: The steam distillation method was used to extract volatile oil from Zedoary Turmeric Oil with concentrations of 40, 80, 120, 160, 200, 240, 280 mg·L-1 to intervene SW1463 cells for 24, 48, 72 h. Thiazolyl blue tetrazolium bromide (MTT) was used to detect the effect on cells proliferation inhibition rate. Giemsal staining was used to observe the effect of different concentrations of Zedoary Turmeric Oil on the morphology of SW1463 cells apoptosis. Enzyme linked immunosorbent assay (ELISA) method was adopted to detect IL-2 and TGF-β1 expression. Western blot assay was used to detect the expressions of Capase-3, MMP-9 and FasL. Result: The MTT assay showed that the oil had a certain inhibitory effect on cell SW1463 proliferation in a time and dose-dependent manner. The half maximal inhibitory concentration (IC50) was 128.49 mg·L-1. With the increase of drug concentrations, microscope observation the cell became smaller and round; Giemsal staining showed typical apoptotic morphological features. The expression of IL-2 and TGF-β1 contents decreased with increase of oil concentrations, single factor analysis showed significant differences in IL-2 expression (P < 0.05) and TGF-β1 expression (P < 0.01). Western blot showed up-regulations in FasL and Capase-3 proteins, but down-regulation in MMP-9 protein, after Zedoary Turmeric Oil processed SW1463 cells for 24 h. Conclusion: Zedoary Turmeric Oil can significantly inhibit SW1463 cells. Therefore the expression of Caspase-3 was negatively correlated with MMP-9.The mechanism may be related to the up-regulation of FasL-induced cellular immune factors.  
        关键词：SW1463;zedoary turmeric oil;interleukin-2;transforming growth factor-β1;FasL   
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        发布时间：2024-01-04
      • YAO Hong-bo,WANG Yue-jing,LIAN Jie,ZHANG Meng,SUN Li-hui
        Vol. 22, Issue 13, Pages: 107-111(2016) DOI: 10.13422/j.cnki.syfjx.2016130107
        摘要：Objective: To observe the effects of compound Rehmanniae Radix on learning and memory abilities, as well as expressions of Wnt pathway associated proteins in Alzheimer's disease (AD) model rats. Method: The 60 healthy Wistar rats were screened by Morris water maze, and randomly divided into 5 groups:normal group, model group, compound Rehmanniae Radix low does(1.35 g·kg-1) group, compound Rehmanniae Radix high does(3.37 g·kg-1) group and galanthamine(0.6 mg·kg-1) group, n=12 in each group. The AD models were induced by injecting Aβ23-35 in the bilateral hippocampus, 10 μL for each side at one time. On 9th day after injection of Aβ23-35, the rats in compound Rehmanniae Radix groups and galanthamine group were given with corresponding medicines by intragastric administration for a course of 30 days. The water maze test was used to evaluate the learning and memory ability in rats, the positioning and quantitative expressions of Wnt pathway associated proteins (Axin) and cell cycle proteins (Cyclin D1) in hippocampus were detected by immunohistochemisty, Western blot and Real-time PCR. Result: As compared with the normal group, the escape latency and the time to first reach the original platform quadrant were significantly more in model group (P < 0.01). As compared with the model group, the escape latency and the time to first reach the original platform quadrant were significantly reduced in galanthamine group and compound Rehmanniae Radix groups (P < 0.05). As compared with galanthamine group, the time to first reach the original platform quadrant was reduced in compound Rehmanniae Radix high dose group (P < 0.05). Immunohistochemisty results showed that, positive expression was in cytoplasm for Axin and in nucleus for Cyclin D1.Western blot and Real-time PCR test results showed that, as compared with the normal group, the protein and mRNA expression levels of Axin in hippocampus were significantly increased in the model group (P < 0.01), as compared with the model group, the protein and mRNA expression levels of Axin in hippocampus were significantly decreased (P < 0.05), while the protein and mRNA expression levels of Cyclin D1 were significantly increased in compound Rehmanniae Radix groups and the galanthamin group (P < 0.05). Conclusion: Compound Rehmanniae Radix can improve the ability of learning and memory in AD rats, and its mechanism may be closed related to reducing the expression of Wnt pathway related proteins Axin and enhancing the expression of Cyclin D1 in hippocampus of AD rats.  
        关键词：Alzheimers disease;compound Rehmanniae Radix;Wnt;Axin;Cyclin D1   
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        发布时间：2024-01-04
      • LIN Han-jie,ZHANG Jin-wei,LU Yue,WEI Jian-an,SUN Jing,LI Li,HAN Ling
        Vol. 22, Issue 13, Pages: 112-117(2016) DOI: 10.13422/j.cnki.syfjx.2016130112
        摘要：Objective: To evaluate the efficacy of Sijunzi decoction in treatment of 2, 4,6-trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis and investigate its effect on expression of tight junction protein Occludin in mucosal epithelial cells. Method: SD rats were divided into normal group, model group (TNBS-induced colitis), Sijunzi Tang(SJZT) high dose group, middle dose group and low dose group (crude drug 5.6, 2.8, 1.4 g·kg-1), and Salazosulfapyridine treatment group (SASP, 0.4 g·kg-1), n=8 in each group(P < 0.05). Experimental ulcerative colitis models were induced by rectal instillation of TNBS in all groups except normal group. 24 h after modeling, drugs were given intragastrically continuously for ten days according to their respective doses in each group. Their diet conditions, mental state, disease activity index (DAI) scores, stool frequency and body weight were observed. 24 h after the last administration, the rats were sacrificed and their colon was taken. The pathological changes of colonic mucosa were visually observed and their scores of colon macroscopic damage index (CMDI) were recorded. Lesion areas were taken for HE staining, and colon tissues damage index (TDI) was evaluated under light microscopy,expression of Occludin proteins in colon of the rats was detected by immunohistochemical method. Result: DAI scores, CMDI scores and TDI scores in model group were higher than those in control group,Occludin protein expression was lower than in control group. However, SJZT and SASP treatment could reduce damage to mucosa of TNBS-induced UC models, increase the expression of tight junction proteins Occludin, inhibit the increase of colonic permeability, and protect the colonic mucosal barrier function(P < 0.05). Conclusion: SJZT can increase Occludin protein expression and promote the recovery of colonic mucosal barrier function in UC rats, which may be one of its mechanism for treatment of colitis.  
        关键词：Occludin;tight junction;ulcerative colitis;mucosal barrier;Sijunzi Tang   
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        发布时间：2024-01-04
      • CHENG Yue,TANG Zhao-hui,CAO Liang,DING Gang,WANG Tuan-jie,ZHANG Chen-feng,WANG Zhen-zhong,XIAO Wei
        Vol. 22, Issue 13, Pages: 118-121(2016) DOI: 10.13422/j.cnki.syfjx.2016130118
        摘要：Objective: To study the effect of Guizhi Fuling capsule and its 15 active components on pelvic inflammation in rat models,and preliminary investigate its treatment effect for pelvic inflammation. Method: Totally 90 female SD rats were divided into sham-operation group and model group. The pelvic inflammatory models were established by Escherichia coli. The successfully modeled rats were further randomly divided into model group,Guizhi Fuling capsule group (250 mg·kg-1) and 15 active components low dose group,middle dose group and high dose group (75,150, 300 mg·kg-1),n=15 in each group. After intragastric administration for 15 d,the rats in Guizhi Fuling capsule group and active components groups were killed. Then their uterus was taken for weighing the uterus and calculating the uterus weight coefficient. Blood was obtained to measure tumour necrosis factor-α(TNF-α),interleukin-2 (IL-2) levels in serum and immune globulinA (IgA),IgG and IgM levels in plasma. Pathological changes of uterine tissues were also detected by htoxylin eosin (HE) staining method. Result: As compared with the sham-operation group,the uterus weight coefficient was significantly increased in rats of model group.IgA,IgG and IgM levels in plasma were significantly reduced,and uterine pathology composite score was significantly increased (P < 0.01). As compared with the model group,the uterus weight coefficient was significantly reduced in pelvic inflammatory rats of Guizhi Fuling capsule group and active components groups,TNF-αlevel in serum was significantly reduced;IL-2 level in serum was increased,the IgA,IgG and IgM levels in plasma were increased;and uterine pathology composite score was also significantly reduced (P < 0.05,P < 0.01). Conclusion: The 15 active components and Guizhi Fuling capsule have significant effects on anti-pelvic inflammatory disease in rats,indicating that the 15 active components may be the main effective ingredients of Guizhi Fuling capsule in the treatment of pelvic inflammatory disease.  
        关键词：Guizhi Fuling Capsule;15 active components;pelvic inflammatory in rat;tumour necrosis factor-α;interleukin-2;immune globulin   
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        发布时间：2024-01-04
      • LIAO Hai-feng,DENG Xiang-liang,LUO Xia,ZHOU Yuan,ZHOU Lian
        Vol. 22, Issue 13, Pages: 122-126(2016) DOI: 10.13422/j.cnki.syfjx.2016130122
        摘要：Objective: To observe the effects of carboxymethylpachymaran (CMP) on the polarization of macrophages. Method: In vivo, Balb/c mice were injected intraperitoneally with CMP (100 mg·kg-1) as CMP group or saline solutions as control group once daily for 5 consecutive days, and phagocytosis of peritoneal macrophages were investigated. In vitro, RAW264.7 cells were treated with CMP (400, 800, 1 600 mg·L-1), lipopolysaccharides (LPS, 0.1 mg·L-1) and incubated for 48 h. The expression of CD86 molecule on cells was tested by flow cytometry. Fluorescent microspheres phagocytosed by RAW264.7 cells were analyzed under fluorescence microscope. Nitric oxide (NO) was detected by Griess reagent, and interleukin-6 (IL-6), interleukin-10 (IL-10), monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF) were investigated by Cytometric Bead Array (CBA). Result: The results in vivo showed that both the phagocytic rate and phagocytic index of peritoneal macrophages in CMP group were higher than those in the control group (P < 0.05). The results in vitro showed that the expression of CD86 on macrophages in CMP and LPS-treated groups were higher than those in the control group (P < 0.05). The results by fluorescence microscope showed that the number of macrophages phagocytosing more than 2 microspheres in CMP and LPS-treated groups were more than those in the control group. Flow cytometric analysis showed that both the phagocytic rate and phagocytic index of RAW264.7 cells in CMP and LPS-treated groups were higher than those in the control group (P < 0.05). LPS significantly induced NO, IL-6, IL-10, MCP-1 and TNF secretions in RAW264.7 cells. On the contrary, all CMP-treated groups did not show the secretions of NO, IL-6, IL-10, MCP-1 and TNF in RAW 264.7 cells, with lower secretions of NO, MCP-1 and TNF than those in the control group. Conclusion: CMP can stimulate the activation and phagocytosis of macrophage, but cannot induce the production of inflammation cytokines, which suggests that CMP might promote the M2 phenotype polarization of macrophages.  
          
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        发布时间：2024-01-04
      • ZHOU Yuan-yuan,LIU Chun-jie,CHU Xian-xiang,LI Chao-yan
        Vol. 22, Issue 13, Pages: 127-130(2016) DOI: 10.13422/j.cnki.syfjx.2016130127
        摘要：Objective: To observe the treatment efficacy of Allicin and effects on tumor necrosis factor-α(TNF-α), sterol regulatoryelement binding protein-1c (SREBP-1c) protein levels in model rats of non-alcoholic fatty liver disease (NAFLD), and to investigate the molecular mechanism. Method: seventy-six male SD rats were randomly divided into normal group (n=12) and model group (n=64). NAFLD models were established by high fat diet, and the rats in model group were further randomly divided into model group, fenofibrate group (50 mg·kg-1·d-1), allicin low dose, middle dose and high dose groups (10, 30, 60 mg·kg-1·d-1), n=12 in each group. The rats in fenofibrate group and allicin groups received corresponding doses of drugs by ig, and the rats in normal group and model group were given with the same volume of normal saline. After 4 weeks of intervention, the animals were sacrificed. The contents of total cholesterol (TG), triglyceride (TC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and liver tissues were detected. The pathological changes of liver tissues were observed by haematoxylin-eosin(HE) staining. TNF-α and SREBP-1c protein expression levels in liver tissues were detected by immunohistochemistry. Result: As compared with the normal group, the TG and TC levels in serum and liver tissues, ALT and AST levels in liver tissues, as well as TNF-α and SREBP-1c protein expression levels in liver tissues were significantly increased in rats of model group (P < 0.05,P < 0.01). As compared with the model group, the TG and TC levels in serum and liver tissues, ALT and AST levels in liver tissues, as well as TNF-α and SREBP-1c protein expression levels in liver tissues were significantly decreased in rats of fenofibrate group, allicin low dose, middle dose and high dose groups (P < 0.05, P < 0.01) Conclusion: Allicin has lipid lowering and liver protective effect in NAFLD model rats induced by high fat diet, and the mechanism may be relevant to reducing the protein expression levels of TNF-α and SREBP-1c.  
        关键词：non-alcoholic fatty liver disease;allicin;tumor necrosis factor-α;sterol regulatoryelement binding protein-1c   
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        发布时间：2024-01-04
      • WANG Ai-mei,CHEN Ya-qi,LI Min,WEI Xiao-ming,XUE Di,WANG Fang,LIU Hong-en
        Vol. 22, Issue 13, Pages: 131-136(2016) DOI: 10.13422/j.cnki.syfjx.2016130131
        摘要：Objective: To investigate the effects of polysaccharide from Corni Fructus on learning and memory ability, protein expression levels of brain derived neurotrophic factor(BDNF) and tyrosine kinase B(TrkB) in hippocampus, and hippocampal long-term potentiation (LTP) of the aging model rats. Method: Totally 40 clean SD rats were randomly divided into normal group, model group, low dose group and high dose group by random number table, n=10 in each group. The models of aging rats were established by subcutaneously injecting D-galactose (140 mg·kg-1·d-1). The rats in normal group and model group received normal saline every day, while the rats in low dose group and high dose group were given with polysaccharide from Corni Fructus(0.14,0.28 g·kg-1·d-1, correspondingly) by intragastric administration for 6 weeks. The learning and memory ability of rats was tested by Morris Water Maze. The Schaffer collateral of hippocampal CA3 region was stimulated by high frequency stimulation (HFS), and LTP in CA1 region of the ipsilateral hippocampus was induced to detect the synaptic plasticity changes of hippocampal neurons in rats. The protein expression levels of BDNF and TrkB in hippocampal neurons were detected by immunohistochemistry. Result: As compared with the normal group, the average escape latency of model group was prolonged significantly, the percentage of swimming distance of the target quadrant in total distance and the frequency of exploration were decreased significantly, the protein expression levels of BDNF and TrkB in the hippocampus were reduced, and the extent of LTP in hippocampus was decreased significantly (P < 0.01). As compared with the model group, the average escape latency of high dose group was decreased significantly; the percentage of swimming distance of the target quadrant in total distance and the frequency of exploration were increased significantly, the protein expression levels of BDNF and TrkB in hippocampus were increased, and the extent of LTP in hippocampus was increased significantly (P < 0.01). Conclusion: Polysaccharides from Corni Fructus may significantly improve the synaptic plasticity in the aging model rats by increasing the protein expression levels of BDNF and TrkB in hippocampus and increasing LTP of hippocampal CA1 region; this may be one of its important mechanisms for improving the ability of learning and memory of the aging rats.  
        关键词：polysaccharides from Corni Fructus;aging;learning and memory;brain derived neurotrophic factor;tyrosine kinase B;long-term potentiation.   
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        发布时间：2024-01-04
      • ZUO Yan-li,JIA Meng-hui,YU Ling-zhi,SU Dan,LI Zhan-tao
        Vol. 22, Issue 13, Pages: 137-141(2016) DOI: 10.13422/j.cnki.syfjx.2016130137
        摘要：Objective: To observe the effect of Shida Lazhi Wan (SDLZW) on Fas and FasL protein and mRNA expressions in the hippocampus of brain tissues of ischemia-reperfusion injury rats. Method: One week after the adaptive feeding, 108 SPF SD rats were divided into 6 groups according to random number table method:control group, ischemia-reperfusion model group (hereinafter referred to as model group), Jinnaduo group, SDLZW low dose group, SDLZW middle dose group, SDLZW high dose group. Based on different reperfusion time, these groups were further divided into 12, 24, 72 h subgroups, with 6 SD rats in each subgroup. Cerebral ischemia-reperfusion model was established by using the modified rat middle cerebral artery (MCA) occlusion method, and reperfusion was performed 2 h after the operation. Jinnaduo group, SDLZW low dose group, SDLZW middle dose group, SDLZW high dose group were given with the corresponding drugs by gavage for the first time 30 min before making cerebral ischemia-reperfusion model, and later administered for 2 times a day with the SDLZW (low dose 11.92 g·kg-1, SDLZW middle dose 23.83 g·kg-1, SDLZW high dose 47.66 g·kg-1) and Jinnaduo (Ginkgo biloba extract, dose 14.44 g·kg-1), with the gavage volume of 10 mL·kg-1, control group and model group were given by gavage with distilled water according to the same method. Brains were collected from each of the three subgroups after 12, 24,72 h. Western blot and Real-time PCR method were adopted to observe the effects of SDLZW on Fas and FasL protein and mRNA expressions in rat brains after cerebral ischemia-reperfusion. Result: Compared with the control group, the expression of Fas and FasL in the model group and all of treatment groups were increased at each time point (P < 0.01), compared with the model group, Fas and FasL expressions at each time point in observed group were reduced (P < 0.01), compared with the Jinnaduo group, the SDLZW middle dose group showed no significant difference in Fas and FasL expressions, and the SDLZW high dose group showed decreases in Fas and FasL expressions (P < 0.05, P < 0.01), especially obviously reduced in the 72 h group (P < 0.01). Conclusion: SDLZW has a good protection on cerebral ischemia-reperfusion injury, and its mechanism may be related to inhibition of apoptosis of hippocampus.  
        关键词：Shida Lazhi pills;ischemia reperfusion;Fas;FasL   
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      • QIU Jian-li,CHAI Yu-na,LI Yue,YANG Hai-die,LI Jie,XU Hua
        Vol. 22, Issue 13, Pages: 142-145(2016) DOI: 10.13422/j.cnki.syfjx.2016130142
        摘要：Objective: To investigate the effect of drug serum of modified Yinchen Sini Tang on transforming growth factor (TGF)-β1/Smads signaling pathways and Collagen3 of TGF-β1-induced rat hepatic stellate cells (HSC). Method: With rat hepatic stellate cells as the study objects, the cells were divided into blank group, model group, positive drug group (methylprednisolone, 10%drug serum), modified Yinchen Sini Tang high dose, middle dose and low dose groups (10%drug serum). All the other rats except those in blank group were given with TGF-β1 (10 g·L-1) and corresponding 10%drug serum culture was used, the effects of the drug serum on protein expression levels of Smad3, Smad7, Collagen3 in HSC-T6 cells were detected by using Western blot assay. Result: As compared with the blank group, protein expression levels of Smad3, Smad7, Collagen3 in HSC-T6 cells were increased in model group, and the increase of Collagen3 expression was more obvious (P < 0.05). As compared with the model group, the protein expression level of Smad3 was significantly decreased in modified Yinchen Sini Tang low dose group (P < 0.05), the protein expression level of Collagen3 was significantly decreased in modified Yinchen Sini Tang middle dose group (P < 0.05). Conclusion: Drug serum of modified Yinchen Sini Tang could down-regulate the protein expression levels of Smad3 and Collagen3, while there was no significant change in Smad7, suggesting that the drug can delay the process of liver fibrosis under the in vitro conditions.  
        关键词：modified Yinchen Sini Tang;Drug Serum;hepatic stellate cells   
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      • YU Tong-yu,GAO Qiao-ying
        Vol. 22, Issue 13, Pages: 146-149(2016) DOI: 10.13422/j.cnki.syfjx.2016130146
        摘要：Objective: To investigate the molecular mechanism of Rhei Radix et Rhizoma on septic rats. Method: Totally 100 Wistar rats were randomly divided into sham-operation group, model group, Rhei Radix et Rhizoma high dose, middle dose and low dose groups (150, 100, 50 mg·kg-1). The rats in sham-operation group only exposed the cecum, without ligation and perforation; and the rats in other groups received cecum ligation method to establish rat sepsis models. The mortality of rats was recorded in various groups. The number of colon and mesenteric lymph nodes, Staphylococcus aureus and Escherichia coli in blood was detected by bacterial 16S rRNA Real-time fluorescence quantitative polymerase chain reaction (qPCR). The levels of zonula occludens-1(ZO-1) and Occludin were detected by qPCR and Western blot. Result: As compared with the sham-operation group, the mortality was the highest in septic model group, the detectable rate and number of S. aureus andE. coli in the samples of septic model group were significantly increased, while ZO-1 and Occludin mRNA and protein expression levels were significantly decreased (P < 0.01). As compared with the model group, the mortality was deceased in Rhei Radix et Rhizoma high dose and middle dose groups; the detectable rate and number of S. aureus andE. coliin the samples of these two groups were decreased, bacterial translocation was reduced, ZO-1 and Occludin mRNA and protein expression levels in rat colon were significantly increased (P < 0.05, P < 0.01). Conclusion: Rhei Radix et Rhizoma can regulate the expression of tight junction protein to protect the intestinal mucosal barrier and inhibit bacterial translocation in septic rats.  
        关键词：Rhei Radix et Rhizoma;zonula occludens;bacterial translocation;sepsis   
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      • HUANG Mei-song,LIU Peng,WANG Shi-cai,HU Yang-qian
        Vol. 22, Issue 13, Pages: 150-155(2016) DOI: 10.13422/j.cnki.syfjx.2016130150
        摘要：Objective: To investigate the effect of ligustilide (LIG) on angiotensin Ⅱ (AngⅡ)-induced cardiac myocytes hypertrophy and its mechanism in neonatal rats. Method: Myocardial cells of SD rats were isolated and purified for primary culture. The cultured myocardial cells were induced by AngⅡ (0.5 mg·L-1) and treated with LIG (various concentrations:20, 40, 80 mg·L-1) for 1 to 3 days. Another blank group was established. Then the cells morphology was observed under inverted microscope and the cells surface area was determined. Total intracellular protein levels were detected by using commercial BCA protein kit and the rate of apoptosis was determined by flow cytometer. The protein expression levels of p53, Bcl-2 and Bax in myocardial cells were determined by Western blot. Result: As compared with the blank group, the cell morphology was obviously changed after exposure to AngⅡ; hypertrophy effect was present in myocardial cells, the cells surface area, total protein levels, and apoptosis rate were increased significantly (P < 0.05). The protein expression levels of p53 and Bax were increased significantly, and the protein expression level of Bcl-2 was decreased significantly (P < 0.05). As compared with AngⅡ alone group, the combination effect of LIG with different concentrations and AngⅡ can ameliorate the AngⅡ-induced hypertrophy of neonatal rat myocardial cells, the cells surface area, total protein levels and apoptosis rate were decreased in a dose-dependent manner (P < 0.05). Conclusion: LIG can inhibit the AngⅡ-induced hypertrophy of cardiomyocytes, and reduce the apoptosis rate maybe by inducing Bcl-2 protein expression and inhibiting p53 and Bax protein expressions.  
        关键词：cardiovascular disease;ligustilide;angiotensin Ⅱ;hypertrophy;apoptotisis   
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      • WANG Jin-bo,LI Zheng,ZHAO Yuan-hong
        Vol. 22, Issue 13, Pages: 156-160(2016) DOI: 10.13422/j.cnki.syfjx.2016130156
        摘要：Objective: To observe the clinical effect of Ganhuangcao compound in the treatment of alcoholic fatty liver disease (AFLD). Method: The 90 patients with alcoholic fatty liver were randomly divided into treatment group and control group, with 45 cases in each group. Both of the two groups were injected with polyene phosphatidyl choline, and the treatment group was additionally given Ganhuangcao compound. The two groups were all treated for 4 weeks, and then their clinical efficacy, liver function, blood lipid, liver imaging changes and adverse drug reactions were observed. Result: The total effective rate was 80%in treatment group, and 60.22%in control group, indicating a statistically significant difference between the two groups (P < 0.05). And the treatment group was superior to the control group in clinical symptoms, liver function and blood lipid (P < 0.05), with statistically significant differences in clinical symptoms, such as fatigue, jaundice, nacha, loose stools, liver function, such as alanine aminotransferase (ALT), gamma-glutamyltranspeptidase (GGT), γ-glutamine based transfer enzyme (γ-GT), and blood lipids, such as low density lipoprotein cholesterol(LDL-C), triglyceride (TG)(P < 0.05). Conclusion: Ganhuangcao compound is effective in treatment of alcoholic fatty liver, and worthy of clinical application.  
        关键词：alcoholic fatty liver;colyenephosphatidyl choline;Ganhuangcao compound;clinical observation;liver function;blood lipids   
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      • ZHANG Hui,WANG Bao-jun,WANG Zhu-feng
        Vol. 22, Issue 13, Pages: 161-164(2016) DOI: 10.13422/j.cnki.syfjx.2016130161
        摘要：Objective: To observe the effect of Suhong lotion in treating articular swelling and pain after osteoarthritis arthroscopic debridement. Method: Totally 90 osteoarthritis patients after arthroscopic debridement during 2010 to 2014 were randomly divided into groups A, B, C. Group A was given with no medicine. Group B was treated with safflower oil for 2 weeks. Group C was externally treated with Suhong lotion for 2 weeks. Special surgery knee (HSS) score, nitrous oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA) were measured among all patients. Result: Articular swelling and pain was lessened, SOD content in synovia was increased, and NO, MDA content in synovia were decreased after washout with Suhong lotion. Conclusion: Washout with Suhong lotion after osteoarthritis arthroscopic debridement lessens articular swelling and pain and improves articular action by increasing SOD content in synovia, and decreasing NO, MDA content in synovia.  
        关键词：osteoarthritis;arthroscopic debridement;special surgery knee score;nitrous oxide;superoxide dismutase;malondialdehyde   
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      • WANG Chun-xia,LI Yong-wei
        Vol. 22, Issue 13, Pages: 165-168(2016) DOI: 10.13422/j.cnki.syfjx.2016130165
        摘要：Objective: To investigate the clinical efficacy of Bailing Tiaogan decoction in treating infertilitas feminis of liver Qi stagnation type polycystic ovary syndrome (PCOS), and observe its effects on levels of free fat acid (FFA), C-reactive protein (CRP) and β-endorphin (β-EP) in serum. Method: One hundred and ten eligible patients were randomly divided into control group (55 cases) and observation group (55 cases) by random number table.Patients of control group were given with ethinylestradiol and cyproterone acetate tablets and clomiphene citrate capsules for ovulation induction. The patients in observation group were treated with Bailing Tiaogan decoction (1 dose/day, bid) by oral administration based on the observation of control group. The treatment course was four menstrual cycles for both groups. Scores of clinical symptoms, endometrial thickness, and ovarian volume were compared between two groups. Clinical pregnancy rate was recorded within six menstrual cycles (MCs) for both groups. The levels of FFA, CRP and β-EP in serum were detected pre-post treatment in two groups. Result: Traditional Chinese medicine (TCM) symptom scores in treatment group were lower than those in control group after treatment (P < 0.01). Within six MCs, the clinical pregnancy rate was 60% in observation group, significantly higher than 36.36%in control group (P < 0.05). The endometrial thickness in treatment group was significantly higher than that in control group after treatment, while the ovarian volume was significantly lower than that in control group (P < 0.01). The levels of FFA and CRP in serum of observation group were significantly lower than those of control group after treatment, while β-EP level was significantly higher than that of control group, with statistically significant differences (P < 0.01). Conclusion: On the basis of control group treatment, Bailing Tiaogan decoction could significantly improve clinical symptoms, increase endometrial thickness, decrease ovarian volume, and increase clinical pregnancy rate in treating infertilitas feminis of liver Qi stagnation type PCOS, and the action mechanism may be related with reducing the FFA and CRP levels and increasing β-EP level in serum.  
        关键词：Bailing Tiaogan decoction;polycystic ovary syndrome;liver Qi stagnation;infertilitas feminis   
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      • FU Guo-qing,WANG Xiang,LI Shu-fang,XIE Liu
        Vol. 22, Issue 13, Pages: 169-172(2016) DOI: 10.13422/j.cnki.syfjx.2016130169
        摘要：Objective: To investigate the clinical effects of Longdan Xiegan Tang and Gegen Fang in assisting western medicine for sudden deafness. Method: The 110 patients with sudden deafness from January 2013 to December 2014 in Hainan Hospital of Traditional Chinese Medicine were selected and randomly divided into control group (55 patients) with conventional western medicine treatment and treatment group (55 patients) with Longdan Xiegan Tang and Gegen Fang based on the treatment in control group. The clinical efficacy,the symptom disappearance rate,the hearing loss values,pure tone threshold value,the level of tinnitus grading state and blood rheology index before and after treatment of two groups were compared. Result: The total effective rate was 80.00% and 90.91% in control group and treatment group respectively. The clinical efficacy in treatment group was significantly superior to that in control group(P < 0.05). The symptom disappearance rate of treatment group was significantly higher than that of control group(P < 0.05). The hearing loss value and pure tone threshold value in treatment group after treatment were significantly lower than those in control group and before treatment (P < 0.05). The level of tinnitus grading state in treatment group after treatment was significantly higher than that in control group and before treatment(P < 0.05). The level of blood rheology index in treatment group after treatment was significantly lower than that in control group and before treatment(P < 0.05). Conclusion: Longdan Xiegan Tang and Gegen Fang in assisting western medicine for sudden deafness can efficiently accelerate the recovery process of hearing,reduce the hearing loss degree and help to alleviate the symptoms of tinnitus.  
        关键词：Longdan Xiegan Tang;Gegen Fang;sudden deafness;clinical efficacy   
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      • NING Yu-feng,YANG Cui lan,LI Fu shan
        Vol. 22, Issue 13, Pages: 173-176(2016) DOI: 10.13422/j.cnki.syfjx.2016130173
        摘要：Objective: To investigate clinical efficacy of Manwei San in treating stomach Yin deficiency syndrome of chronic atrophic gastritis(CAG) and its influence on serum levels of Proteinase Ⅰ(PGⅠ) and gastrin(GS). Method: One hundred ten CAG cases were were randomly divided into observation group(55 cases) and control group (55 cases) referring to random number table.Cases in control group received Vitacoenzyme tablets 0.2 g (4 tablets/time, tid).Patients in observation group was treated with Manwei san,and Chinese herbal medicines were smashed and orally took with water(9 g/time, tid).Course of treatment was for 3 months for all cases.Scores of symptoms of traditional Chinese medicine(TCM) and pathology scores of gastric mucosa were compared between both groups pre-post treatment,and clinical efficacy was analysed after treatment.Serum levels of PGⅠand GS were detected in two groups before and after treatment. Result: Scores of TCM in observation group were obviously lower than those of control group(P < 0.01).The total efficacy of observation group was 90.74%,and control group was 75.93%with statistical difference between both groups (P < 0.05).After treatment,scores of inflammation of the mucosa,inflammation activity,reducing of gland,and intestinal metaplasia were remarkably lower than control group(P < 0.01).Compared to control group,serum levels of PGⅠand GS in observation group was obviously increased with statistical difference after treatment(P < 0.01). Conclusion: Manwei san could evidently improve clinical symptoms and signs,pathology scores of gastric mucosa,and clinical efficacy in treating stomach Yin deficiency syndrome of CAG,and its action may be related with elevating PGⅠand GS content in serum.  
        关键词：chronic atrophic gastritis;Manwei San;stomach Yin deficiency syndrome;proteinase Ⅰ;gastrin   
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      • SHU Jian-zhong,TANG Jun,LI Chen-yu,XUE Hong,LIU Jian-hui
        Vol. 22, Issue 13, Pages: 177-181(2016) DOI: 10.13422/j.cnki.syfjx.2016130177
        摘要：Objective: To investigate the clinical efficacy of Jieyu Anshen particles combined with paroxetine hydrochloride tablets in treatment of depression after ischemic stroke its effects on levels of interleukin (IL)-6, IL-23, tumor necrosis factor-α(TNF-α), norepinephrine (NE) and 5-hydroxytryptamine(5-HT) in serum. Method: Ninety-four patients with depression after ischemic stroke in our hospital were elected and randomly divided into observation group (48 cases) and control group (46 cases) according to random number table. All patients in both groups were treated with conventional intervention according to ‘Guidelines for Stroke Rehabilitation in China’. Patients in control group orally took paroxetine hydrochloride tablets, 20 mg/day at beginning, increased by 10 mg per week, and with a maximum dose≤40 mg/day. Patients in observation group were given with Jieyu Anshen particles based on the treatment of control group, 5 g/time, bid. Patients in both groups were treated for 6 weeks. Scores of Hamilton's depression scale (HAMD), scores of HAMD factors, and clinical efficacy were compared between two groups. The levels of IL-6, IL-23,TNF-α, NE and 5-HT in serum were detected in both groups. Result: After 3 and 6 weeks' treatment, scores of HAMD scale in observation group were lower than those in control group (P < 0.01). Scores of HAMD factors in observation group were lower than those in control group after treatment (P < 0.01). The total effective rate was 91.67%in observation group, higher than 71.74%in control group (P < 0.05). After treatment, the levels of IL-6, IL-23,and TNF-α in serum of observation group were significantly lower, while NE and 5-HT levels were significantly higher than those of control group, with statistically significant differences (P < 0.01). Conclusion: Jieyu Anshen particles combined with hydrochloride tablets had definite efficacy in treatment of depression after ischemic stroke, and the therapeutic effect may be related with down-regulating the levels of IL-6, IL-23, TNF-α, and up-regulating NE and 5-HT levels in serum.  
        关键词：Jieyu Anshen particles;paroxetine hydrochloride tablets;ischemic stroke;depression   
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      • ZHOU Qian-yi,HUANG Chao-yuan,WENG Xiang-tao,WU Yu-yun,ZHANG Shi-jing,LI Shao-shuo,CHEN Xin-lin
        Vol. 22, Issue 13, Pages: 182-186(2016) DOI: 10.13422/j.cnki.syfjx.2016130182
        摘要：Objective: To review the methodological quality of systematic review/Meta-analysis(SR/MA) of Shuxuetong in treating stroke. Preferred reporting items for systematic reviews and Meta-analysis(PRISMA) entries and Assessment of Multiple Systematic Reviews(AMSTAR) scale were used to evaluate and analyze the quality of the methodology of the literatures. Method: Chinese Biomedical Literature, China National Knowledge Infrastructure, VIP, Wanfang and PubMed databases were searched to collect literatures for systematic review/Meta-analysis of Shuxuetong in treating stroke. The search time was as at May 2015. Result: Ten articles were included. The average PRISMA score was 22 scores, including one article (10%) with severe deficiency. The AMSTAR scale showed 6 scores on average. The main problems included bias in registered scheme, choice of researches, data extraction and methodology, and no source of fund. Conclusion: The systematic review/Meta analysis for the methodological and report quality of Shuxietong in treating stroke is relatively complete. But it still need to further improve the regimen design, control the quality of prospective literatures, report the sources of fund, and standardize the methodological quality.  
        关键词：Shuxuetong;stroke;Meta-analysis;preferred reporting items for systematic riviews/Meta-analysis;Assesment of Multiple Systematic   
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      • HUANG Chao-yuan,SUN Shu-ning,LU Yang,LIU Min
        Vol. 22, Issue 13, Pages: 187-194(2016) DOI: 10.13422/j.cnki.syfjx.2016130187
        摘要：Objective: To systematically review the effectiveness and safety of Xuefu Zhuyu Tang combined with western medicine in the treatment of diabetic peripheral neuropathy (DPN). Method: Such databases as Pubmed, Embase, Cochrane library, CNKI, CBM, VIP database and Wanfang data were searched to collect randomized clinical trials on the effectiveness and safety of Xuefu Zhuyu Tang combined with western medicine in the treatment of DPN. According to the inclusion and exclusion criteria, literatures were screened, data was extracted, and the methodological quality of included studies was also assessed. Then, a Meta-analysis was performed using RevMan 5.3 software. Result: The 23 RCT were included eventually, involving a total of 1 933 cases treated with Xuefu Zhuyu Tang combined with western medicine in the treatment of DPN. The analysis shows that the effectiveness and safety of Xuefu Zhuyu Tang combined with western medicine in the treatment of DPN were superior to those of pure western medicine. The treatment group showed a total effective rate of[RR=1.27, 95%CI(1.21, 1.33), P < 0.001], median nerve motor merve conduction velocity(MNCV) of[MD=5.28, 95%CI(3.16,7.41), P < 0.001], median nerve sensory nerve conduction veldity(SNCV) of[MD=3.66, 95%CI(1.78, 5.55), P=0.001], common peroneal nerve MNCV of[MD=6.97, 95%CI(4.59, 9.35), P < 0.001], common peroneal nerve SNCV of[MD=3.68, 95%CI(2.41, 4.95), P < 0.001], ulnar nerve MNCV of[MD=4.44, 95%CI(0.96, 7.91), P=0.01], ulnar nerve SNCV of[MD=2.83, 95%CI(-0.55,6.21), (P=0.10)], tibial nerve MNCV of[MD=4.05, 95%CI(3.09, 5.01), P < 0.001],and tibial nerve SNCV of[MD=4.21, 95%CI(2.36, 6.07), P < 0.001]. Conclusion: The effectiveness and safety of Xuefu Zhuyu Tang combined with western medicine in the treatment of DPN in the treatment group is as the same as the control group. But the existing researches need to be verified in more high-quality randomized and double-blind multicenter studies due to their poor quality.  
        关键词：Xuefu Zhuyu Tang;western medicine;diabetic peripheral neuropathy;Meta-analysis   
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      • LI Tao,ZHOU Li-mei,CUI Lu,ZHOU Xin-xin
        Vol. 22, Issue 13, Pages: 195-201(2016) DOI: 10.13422/j.cnki.syfjx.2016130195
        摘要：Objective: To find a new method in exploring traditional Chinese medicine (TCM) compounds,with the classical Chinese medicine prescription Buzhong Yiqi Tang (BD) for myasthenia gravis (MG) as the example. Method: In our study,acetylcholinesterase (AChE) was first supposed as the target,and then the TCM prescriptions' chemical component database and acetylcholinesterase inhibitors (AChEi) database were established by data mining method. With the application of Lipinski's five rules,composite technology of molecular two dimensional (2D) similarity and three dimensional similarity (3D),as well as Glide molecular docking technology,virtual screening of the candidate AChEi from the TCM prescriptions' chemical component database was done and verified by experiment. Result: The mapping relationship between the compounds and targets were established in this study to predict appropriate targets,and thus find the key active compounds and their chemical structures. Furthermore,5 new compounds with new AChEi structures were found in the experiment. Conclusion: This study provides new direction and new method for research on TCM compounds as well as the development and research of targeted drugs,which is important for innovation and modernization of Chinese medicine.  
        关键词：myasthenia gravis (MG);Buzhong Yiqi Tang;drug screening;acetyl cholinesterase inhibitors (AChEi)   
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      • CHEN Jie,DING Wei-jun
        Vol. 22, Issue 13, Pages: 202-207(2016) DOI: 10.13422/j.cnki.syfjx.2016130202
        摘要：Recurrent oral ulcer (ROU) is a common disease of oral mucosa, with a high incidence rate and complicated etiology. It is correlated with many factors, such as abnormal immune function, oral flora disorder, infection, microcirculation disturbance, trace element deficiency and endocrine disorders, but the exact cause and pathogenesis are still unknown. The imbalance of oral micro-ecosystem and immune system is a hotspot in current studies. Oral microbial imbalance has been paid more and more attention by domestic and foreign scholars, and the studies on oral bacteria have some progress. However, viral genome, which is the most abundant member of oral microbial community, has not been further studied. With the great advantages of virus macro genomics in the medical field, high throughput sequencing method has been rapidly promoted in the field of viral genomics studies, and grown into a powerful tool for solving virus-host interactions. According to traditional Chinese medicine (TCM), ROU is also known as recurrent aphthous, whose etiology and pathogenesis are dominated by 'inflammation'. The inflammatory syndromes in spleen and stomach are the most common, which is closely related to the imbalance of oral micro-ecosystem and immune disorders. Study on micro ecological regulation, as an advantageous field of TCM, starts with oral viral genome and TCM's therapeutic mechanism for ROU, and focuses on core molecular mechanisms of ROU through high throughput sequencing method. It is expected to observe dynamically oral viral genomes regulation mode of TCM compounds, and reveal TCM's micro-ecological connotation reaching clinical curative effect. It is of important significance to fully understand the ROU pathogenesis, and give better guidance to clinical treatment.  
        关键词：recurrent oral ulcer;imbalance of oral micro-ecosystem;immune system;traditional Chinese medicine   
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      • LYU Shu-quan,ZHANG Shu-fang,SU Xiu-hai,SONG Hui-li,LIU Ai-ru
        Vol. 22, Issue 13, Pages: 208-212(2016) DOI: 10.13422/j.cnki.syfjx.2016130208
        摘要：Diabetic peripheral neuropathy (DPN) is the most common chronic complications of diabetes, and one of microvascular complications. Modern medical studies have not defined the pathogenesis, and generally believed that high blood sugar-induced oxidative stress plays a key role. Modern medicines for hypoglycemic, nutrition, improving microcirculation, inhibiting sorbitol production and resisting oxidation have showed a certain clinical curative effect. However, here is still no effective method to treat stubborn painful neuropathy, which could still be relieved with analgesic drugs. In recent years, traditional Chinese medicine (TCM) has made great progress in clinical treatment of the disease, with further researches on the mechanism of action of TCM. Nearly 10 years of literatures were retrieved to summarize the etiology and pathogenesis, syndrome type differentiation, specially designed medicine,TCM combined with western medicine, combination of acupuncture with medicine and experimental researches. According to the studies, TCM believes that this disease belongs to a ‘collateral disease’, and mainly treated with therapies for tonifying Qi and Yin, warming Yang, promoting blood circulation to remove blood stasis, and dredging collaterals, and combination of internal and external treatment could show the multi-target effect. But the current researches mostly focused on a specific prescription or medicine, and paid more attention to observation indexes in blood sugar, blood fat, superoxide dismutase (SOD), malondialdehyde (MDA), and nerve conduction velocity than the molecular and cellular indexes. Therefore, how to define the mechanism of action of TCM in treatment of DPN and conduct concentration-response relationship and toxicology researches by using modern technologies under the guidance of TCM theory is a key problem to be solved.  
        关键词：diabetic peripheral neuropathy;high blood sugar induced;oxidative stress   
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        发布时间：2024-01-04
      • CHU Bo-wen,ZHANG Qi,LI Zhi-min,WANG Yuan-zhong
        Vol. 22, Issue 13, Pages: 213-222(2016) DOI: 10.13422/j.cnki.syfjx.2016130213
        摘要：Objective: Gentianarigescens,mainly distributed in Yunnan and Sichuan province of China, is widely known as traditional Chinese herbs in folk with long medicinal history. G. rigescens (Gentianaceae), is a kind of perennial herb, whose chemical constituents mainly include iridoids, triterpenes, lignans, flavanoids, alkaloids, gentisides and others. Their main pharmacological effects include liver protection, choleretic effect, analgesia, anti-inflammatory, anti-oxidant, promoting neural activation and so on.This paper would summarize the chemical constituents and pharmacological activity of G. rigescens, and provide reference for further development and utilization of G. rigescens.  
        关键词：Gentiana rigescens;chemical constituent;pharmacological activity;research advances   
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        发布时间：2024-01-04
      • GAO Ming-zhou,WANG Jie-qiong,AN Li,ZHANG Hao,GUO Kai-yuan,GAO Dong-mei
        Vol. 22, Issue 13, Pages: 223-228(2016) DOI: 10.13422/j.cnki.syfjx.2016130223
        摘要：There are corresponding special affinity between specific physical character and pathogenic factors, which is consistent with the origin-analogue theory. With transition from the biomedical model to the biological-psychology-social medical model, the community medicine is shifting to the individual medicine, and individual differences are getting more and more attention. As an important part of life science, the science of traditional Chinese medical(TCM) constitution pays attention to regulating functions of human body as a whole, as well as individual differences in constitution. And constitution is an important form of human life activities, as well as the combination of specific physical quality and psychological quality. On the basis of descriptions about personality and constitution in the Inner Canon of Huangdi, the TCM concept of the ‘integration of body and spirit’, the TCM theory of personality and constitution opens up a new perspective for disease control and prevention. Nowadays, premenstrual syndrome affects life of patients in all aspects. Due to the special constitutions of women of childbearing age, personality becomes the research emphasis of the disease. This article focuses on a brief analysis on the relationship between the physical character and premenstrual syndrome, in order to provide prevention and control measures from the perspective of the constitution and personality, improve personal physique, achieve the health status with the balance of Yin and Yang, and carry forward the theory of preventive treatment.  
        关键词：constitution;personality;premenstrual syndrome;pathogenesis;balance of Yin and Yang   
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        发布时间：2024-01-04
      • YANG Xiao-xia,LIN Hua-qing,LUO Jin-jie
        Vol. 22, Issue 13, Pages: 229-234(2016) DOI: 10.13422/j.cnki.syfjx.2016130229
        摘要：Soft capsules,which are made by vegetable gum replacing the gelatin as the capsule shell,are called vegetable gum soft capsules.With the deep application of gelatin soft capsule,its disadvantages become more prominent,so it is necessary to find a new capsule material for the development of soft capsule.Currently,vegetable gum soft capsules has made some progress with many patent applications,but few mature products come into the market.Based on consulting a large number of related literatures of vegetable gum soft capsules in recent years,this paper classified five kinds of soft capsule materials which has mature research,including the research status of carrageenan,starch,mannan gum,xanthan gum and sodium alginate,and their gel mechanism and advantages are discussed.Therefore,this article will discuss with the prospect of its application prospects and proposing further research,provide a reference for the development and application of new vegetable gum soft capsules.A variety of excellent performance of vegetable gum soft capsules were mixed to make up for the defects when used alone,thereby expanding the scope of use of vegetable gum soft capsules and improving its performance.  
        关键词：vegetable gum soft capsules;capsule materials;carrageenan;starch;mannan gum;xanthan gum;sodium alginate   
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