最新刊期
卷 22 ， 期 11 ， 2016
- 摘要：Objective: To clarify pharmacodynamic material basis of salt-processed Alpiniae Oxyphyllae Fructus by investigating the contribution of chemical components from petroleum ether fraction of salt-processed products to the effect on detrusor in bladder of Guinea pig. Method: The fingerprint of petroleum ether fraction of salt-processed Alpiniae Oxyphyllae Fructus was established by HPLC, the mobile phase consisted of water and acetonitrile for gradient elution. Effect of petroleum ether fraction on detrusor in bladder of Guinea pig was investigated, and its material basis was analyzed, grey relational analysis method was used to establish the relationship between spectrum and efficacy. Result: The fingerprint of petroleum ether fraction of salt-processed products was established with similarity > 0.9. Detrusor inhibitory effect was the result of many kinds of components, the contribution of different peaks to relieve polyuria was ranked as 20 > 9 > 11 > 17 > 16 > 13 > 15 > 24 > 18 > 23 > 14 > 32 > 34 > 22 > 25 > 19 > 31 > 27 > 21 > 29 > 28 > 30. Conclusion: The HPLC fingerprints is simple and repeatable. The relationship between fingerprints of petroleum ether fraction and efficacy is paralleled to some extent, and this study will lay a foundation for the further research on inhibitory of salt-processed Alpiniae Oxyphyllae Fructus for polyuria.关键词：Alpiniae Oxyphyllae Fructus;salt-processed products;fingerprint;detrusor of bladder;spectrum-effect relationship;arresting polyuria;nootkatone
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components in raw and processed products of Salviae Miltiorrhizae Radix et Rhizoma by high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Method: Separation was performed on a Halo® C18 column (2.1 mm×100 mm, 2.7 μm), the mobile phase consisted of acetonitrile and 0.1% formic acid water with gradient elution. TOF-MS with electrospray ionization (ESI) ion source was applied for qualitative analysis under positive ion mode. Combining with standard reference positioning, MS data, literatures and database matching, ion peak number and peak area of Salviae Miltiorrhizae Radix et Rhizoma before and after being processed were compared. Result: Sixteen components and fourteen components were obtained from raw and processed Salviae Miltiorrhizae Radix et Rhizoma, respectively. After being processed with wine, the peaks of lithospermic acid or salvianolic acid H and tanshinone ⅡB were disappeared. Peak areas of cryptotanshinone, neotanshinone B, tanshinone ⅡA and miltirone decreased significantly. Peak areas of dihydrotanshinone Ⅰ and tanshinone Ⅰ increased. Conclusion: Changes of type and content of chemical components in Salviae Miltiorrhizae Radix et Rhizoma are remarkable after being processed with wine, part of tanshinones and salvianolic acids have qualitative and quantitative change. It speculates that enhance of blood stasis is related to chemical components of Salviae Miltiorrhizae Radix et Rhizoma transformed into the active components which can be absorbed easily.关键词：Salviae Miltiorrhizae Radix et Rhizoma;processed products with wine;tanshinones;salvianolic acids;miltirone;HPLC-TOF/MS
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of Shaoshang Yuhe hydrogel based on its pharmacodynamic index and determine the content of major pharmacological compositions in this preparation. Method: Hydrogels were prepared by different process, and the scald model in rats were used to evaluate efficacy by observing improvement of pathological structural and healing in wound area, measuring the leves of serum tumor necrosis factor-α, interleukin-1β and myeloperoxidase. HPLC and GC were utilized to measure the content of major pharmacological compositions in hydrogel and cream with the original process, respectively. Result: After treatment of 21 d, the wound areas of rats in Shaoshang Yuhe cream group, half extracted hydrogel group and Shirun Shaoshang cream group were (0.28±0.15), (0.31±0.36), (0.29±0.17) cm2, respectively. While effects of half extracted hydrogel group was better than Shaoshang Yuhe cream on reducing the infiltration degree of inflammatory cell and decreasing the content of three inflammatory factors, and it was closed to the Shirun Shaoshang cream group. The contents of three alkaloids as berberine hydrochloride, palmatine hydrochloride and coptisine hydrochloride in the hydrogel were 1.609, 0.247, 0.335 mg·g-1, respectively, there were higher than these in Shaoshang Yuhe cream. The contents of four volatile components as α-pinene, limonene, isoborneol and borneol were 0.052, 0.149, 1.438, 1.643 mg·g-1, respectively, there were higher than these in the same batch of Shaoshang Yuhe cream. Conclusion: In addition to wound healing area, other pharmacodynamic indexes and the contents of effective components of the hydrogel by the optimized preparation process are better than the cream.关键词：Shaoshang Yuhe hydrogel;Shaoshang Yuhe cream;pharmacodynamic index;alkaloids;α-pinene;limonene;isoborneol
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发布时间：2024-01-04 - 摘要：Objective: To explore a decalcification method with easier operation and shorter time. Method: Forty DBA/1 mice were randomly divided into normal group and model group. Mice in model group were induced with type II collagen. Ankles were achieved from both model group and normal group, and each of groups were demineralized by improved method and conventional method, respectively. Effect of these two decalcification methods was compared from three aspects, including joint pathology, pathological scores and immunohistochemistry. Result: When the temperature was 4℃ and the replacement cycle of decalcification fluid was 24 h, the improved decalcification speed was 7 d, and this operation did not have an obvious effect on the structure and the protein expression of bone tissue. Conclusion: This optimized decalcification method can effectively improve the decalcification speed, which can get pathological diagnosis rapidly.关键词：bone tissue sections;EDTA decalcification;collagen-induced arthritis;immunohistochemistry;decalcific temperature
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发布时间：2024-01-04 - 摘要：Objective: To optimize the preparation process of inclusion complex of volatile oil from Acori Tatarinowii Rhizoma and hydroxypropyl-β-cyclodextrin (HP-β-CD). Method: Taking composite score of inclusion rate of volatile oil, yield of inclusion complex and the content of α-asarone as index, central composite design-response surface methodology was adopted to optimize inclusion process with inclusion temperature, reaction time, ratio of HP-β-CD and volatile-oil as factors. The quality of inclusion complex was evaluated by infrared spectroscopy (IR) and GC-MS. Result: The best process was as follows, inclusion time was 3.85 h, inclusion temperature was 30.06℃, ratio of HP-β-CD and volatile-oil was 8.69: 1. Under these conditions, inclusion rate of volatile oil was 79.74%, yield of inclusion complex was 87.47%, the content of α-asarone in inclusion complex was 2.266 mg·g-1. After inclusion, similarities of GC-MS spectra of volatile oil was high. Conclusion: The characterization results of IR and GC-MS proved the generation of inclusion complex. Central composite design-response surface methodology is suitable for optimizing inclusion process of inclusion complex of volatile oil from Acori Tatarinowii Rhizoma and HP-β-CD.关键词：Acori Tatarinowii Rhizoma;volatile oil;hydroxypropyl-β-cyclodextrin;α-asarone;infrared spectroscopy;gas chromatography-mass spectrometry
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发布时间：2024-01-04 - 摘要：Objective: To study on total polysaccharides in Taraxaci Herba and its in vitro anti-inflammatory activity for screening active ingredient of anti-inflammatory. Method: Water extract-alcohol precipitation method was adopted to gain total polysaccharides in Taraxaci Herba (TMP), and then it was purified by DEAE cellulose column to get three parts of TMP (TMP-0, TMP-1, TMP-2). Anti-inflammatory activity of TMP on RAW264.7 cells induced by LPS was investigated. Result: TMP can significantly reduce the mRNA expression of inflammatory factors [cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β(IL-1β)] in RAW264.7 cells with a dose-dependent manner. The two fractions, TMP-0 and TMP-1, can significantly reduce the mRNA of these above inflammatory factors, they showed a good in vitro anti-inflammatory activity, while the anti-inflammatory effect of TMP-2 at low and middle doses was not obvious. Conclusion: TMP has a strong anti-inflammatory activity, and its main active fractions are TMP-0 and TMP-1.关键词：Taraxaci Herba;total polysaccharides;DEAE;anti-inflammatory;RAW264.7 cell;elution fraction
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发布时间：2024-01-04 - 摘要：Objective: To optimize the conditions of supercritical CO2 extraction and ethanol extraction processes of yolk oil. Method: HPLC-ELSD was employed to determine the content of phosphatidylcholine with mobile phase A of methanol-water-glacial acetic acid-triethylamine (85: 15: 0.45: 0.05) and mobile phase B of n-hexane-isopropanol-mobile phase A (20: 48: 32) for gradient elution. Based on single factor tests, taking yield of yolk oil and the content of phosphatidylcholine as indexes, orthogonal tests were adopted to optimize supercritical CO2 extraction and ethanol extraction processes of yolk oil. Result: Optimum extraction conditions were as follows, moisture content of yolk oil was ≤5%, supercritical CO2 extraction temperature was 50℃, pressure was 35 MPa, flow was 50 L·h-1, extraction time was 2 h, yield of yolk oil was 42.65%, residual powder of supercritical CO2 extraction was extracted four times with four times the amount of 90% ethanol for 1.5 h of each time, the content of phosphatidylcholine was up to 17.24%. Conclusion: These optimized technologies are stable and feasible for development and application of yolk oil.关键词：supercritical CO2extraction method;yolk oil;phosphatidylcholine;lecithin;moisture content
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction conditions of total nucleosides in Codonopsis tangshen. Method: Ultrasonic method was adopted to extracting total nucleosides in C. tangshen. Taking the contents of total nucleosides (cytidine, uridine, guanosine and adenosine) as index, based on single factor tests, orthogonal test was designed to optimize extraction conditions of total nucleosides in C. tangshen. HPLC was employed to determine the contents of cytidine, uridine, guanosine and adenosine with mobile phase of methanol-water for gradient elution and detection wavelength at 260 nm. Result: The influence of various factors on the extraction rate of total nucleosides was on order of volume fraction of methanol > extraction times > solid-liquid ratio > extraction time. Optimal parameters was as follows:extracted twice with 10 times the amount of water for 30 min of each time. Under these conditions, extracting amount of total nucleosides was 1.523 mg·g-1. Conclusion: This established HPLC method is accurate and reliable to detect contents of total nucleosides. At the same time, this optimized extraction technology is stable and feasible with the advantages of energy-saving, rapidity, efficient and so on.关键词：Codonopsis tangshen;ultrasonic extraction;nucleosides;cytidine;uridine;guanosine;adenosine
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发布时间：2024-01-04 - 摘要：Objective: To provide the scientific basis for the reasonable utilization and development of medicinal plant resources on Puti island. Method: In-depth research on the types, compositions and medicinal values of the medicinal plant resources on Puti island was conducted through field inspection, sample collection, data accessing and samples identification. Result: A total of 51 families 96 genera and 108 species of wild medicinal herbs were found in the study. Among them, the dominant families were Asteraceae, Liliaceae, Labiatae, Asclepiadaceae, Rosaceae, Gramineae, Leguminosae and Brassicaceae, accounting for 47.23% of total medicinal plants on Puti island. The medicinal parts and efficacy of the medicinal plants on Puti island were statistically studied, and the wild medicinal plants with great development values were briefly introduced in this study. Conclusion: Puti island has rich resources of wild medicinal plants, with great value of development and utilization.关键词：Puti island;wild medicinal plant resources;dominant family;medicinal part and efficacy;development and utilization
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发布时间：2024-01-04 - 摘要：Objective: To compare the difference of HPLC fingerprints of commercially available Hippophae Fructus pieces, explore the correlation between their HPLC fingerprints and antioxidant activity, and provide reference for exploring the antioxidant material basis of Hippophae Fructus as well as quality evaluation. Method: HPLC fingerprints of different sources of Hippophae Fructus pieces were established, and the difference were analyzed by the similarity and system cluster analysis method;antioxidant effect was determined by 1, 1 diphenyl-2-trinitrobenzene hydrazine (DPPH) method, and the spectrum-effect relationship was studied by gray correlation analysis method. Result: The HPLC fingerprints of 14 batches of Hippophae Fructus were established;10 common peaks were identified, and No. 10 peak was the chromatographic peak for quercetin;the samples came into two categories, with the similarity of 0.862-0.992;the 14 batches of Hippophae Fructus pieces had different levels of free radical scavenging effect;the correlation between 10 common peaks and free radical scavenging was in the range of 0.758-0.662, with an order of x10 > x3 > x7 > x4 > x5 > x9 > x1 > x6 > x8 > x2 from high to low, where No. 10 peak for quercetin had the maximum correlation with DPPH free radical scavenging effect. Conclusion: The quality of commercially available Hippophae Fructus was relatively consistent, but there were some differences in quality between those from different regions, and their antioxidation effect was resulted from the common onset of 'multi-components'. By exploring the antioxidant material basis of Hippophae Fructus through the study of HPLC fingerprints consistency and spectrum-effect relationship analysis, it can provide reference for quality evaluation of Hippophae Fructus.关键词：Hippophae Fructus pieces;HPLC fingerprint;consistency;antioxidant;spectrum-effect relationship
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical components from Prunellae Spica and their anti-tumor activities in vitro. Method: Extracts were separated and purified by column chromatography over silica gel, octadecylisilyl(ODS), Sephadex LH-20, and the component structures were identified by physical and chemical properties and spectral methods including 1H-NMR and 13 C-NMR. MTT method was used to separately establish blank group, positive drug group (different concentrations of cisplatin), and experimental drug groups at different concentrations;and measure the anti-tumor activities of these chemical components against MCF-7, MDA-MB-231 breast cancer cells and MCF-10A normal breast cell lines. Result: The 11 compounds were isolated and identified from the fruits of Prunellae Spica, and their structures were identified as:trans-salviaflaside (1), trans-salviaflaside methyl ester (2), rosmarinic acid (3), 3-(3, 4-dihydroxyphenyl)acrylic acid 1-(3, 4-dihydroxy-phenyl)-2-methoxycarbonylethylester (4), caffeic acid-3-O-glucoside (5), danshengsu (6), 3, 4, α-trihydroxy-mephenylpropionate (7), 3, 4-dihydroxybenzaldhyde (8), (3R, 5S, 6S, 7E, 9R)-megastigman-7-ene-3, 5, 6, 9-tetrol-9-O-β-D-glucopy-ranoside (9), (-)-syringaresinol-4-O-β-D-glucopyranoside (10), and 16-oxygen-17-demethyl-ation-3β, 24-hydroxy oleanolic acid-12-en-3-O-β-D-glucuronic acid (11). Compound 4 showed an inhibitory rate over 88% against all the cells, compounds 2 and 8 showed an inhibitory rate over 60% against the cell lines MCF-7 and MCF-10A, compound 3 showed an inhibitory rate over 60% only against the MCF-7. Conclusion: Compounds 9 and 10 were obtained from the Prunellae Spica for the first time. The results of anti-tumor assay indicated that compounds 2-4 and 8 had significant inhibitory effect against breast cancer cell MCF-7, compound 4 had strong inhibitory effect against breast cancer cell MDA-MB-231;but the compounds 2, 4 and 8 also showed inhibitory effect against normal breast cell lines, and compound 3 can selectively inhibit the cancer cells.关键词：Prunellae Spica;chemical components;anti-tumor activities;breast cancer cells
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for the determination of agaropiric acid in artificial agarwood and study the correlation between agaropiric acid and extract contents. Method: The content of ethanol-soluble extract was determined according to those correlative standards of Aquilariae Lignum Resinatum in Chinese Pharmacopoeia (2015 Version);HPLC was done on CNW Athena C18-WP column (4.6 mm×250 mm, 5 μm), with acetonitrile-0.1% phosphoric acid solution (40: 60) as the mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 217 nm and the column temperature was 35℃. Result: The linear range of agaropiric acid was 0.24-24 mg·L-1 (r=0.999 7). RSD was 0.4% for precision degree and 0.9% for stability within 24 h, the average mass fraction of agaropiric acid was 27.86 μg·g-1, and its average recovery rate was 96.4% with RSD of 2.8%. Conclusion: This HPLC method is accurate for determination of agaropiric acid content. The average content of agaropiric acid was highest in comprehensive way, and the content of agaropiric acid is increased with the increase of ethanol-soluble extract content. This correlation can provide the relativity of contents between the ethanol-soluble extract and agaropiric acid, and provide a reference for quality evaluation of artificial agarwood.关键词：artificial Aquilariae Lignum Resinatum;agaropiric acid;ethanol-soluble extract;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the quality of Semen Armeniacae Amarum collected from various habitats. To establish an HPLC fingerprint of Semen Armeniacae Amarum collected from various habitats, and to make evaluation by principal component analysis and cluster analysis. Method: Inertsil ODS-3 C18 column (4.6 mm×250 mm, 5 μm) was used with acetonitrile-0.05% phosphoric acid solution as mobile phase for gradient elution. The detective wavelength was 215 nm and the flow rate was 1.0 mL·min-1. HPLC fingerprints of Semen Armeniacae Amarum from ten different sources were set up. Similarity evaluation combined with hierarchical clustering analysis (HCA) and principal components analysis (PCA) were used to evaluate the quality of Semen Armeniacae Amarum herb from ten different sources. Result: The common mode of HPLC fingerprints for Semen Armeniacae Amarum was established and 12 common peaks were marked in the fingerprints. The cluster analysis and principal component analysis were applied for pattern recognition. Conclusion: The established fingerprints method provides scientific foundation for the reasonable collection and quality evaluation of Semen Armeniacae Amarum. The Semen Armeniacae Amarum collected in Hebei province has better quality.关键词：Semen Armeniacae Amarum;HPLC;fingerprint;source
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发布时间：2024-01-04 - 摘要：Objective: To analyze the volatile components in Hypericum perforatum from Hebei province. Method: The volatile oil was extracted by steam distillation method and its chemical constituents were analyzed and identified by gas chromatography-mass spectrometry (GC-MS) technology. Result: The 58 compounds were isolated and identified from the volatile oil of H. perforatum, representing 90.0% of the total amount of volatile oil. The main constituents were identified as linalool (14.66%), 4-methoxypropenylbenzene (6.89%), benzeneacetaldehyde (4.66%), mushroom alcohol (4.41%), and viridiflorol (4.38%). Conclusion: The volatile oil of H. perforatum from Hebei province was significantly different from that grown in other areas, indicating that the chemical constituents could be greatly influenced by various geographical locations and environmental conditions. It provides foundation for the rational development and utilization of this plant.关键词：Hypericum perforatum;volatile;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the branches and leaves of Sabina gaussenii. Method: Silica gel, Sephadex LH-20, RP-18, MCI-gel column chromatography, semi-preparative HPLC and other means were used to isolate an purify the constituents, the structures of the compounds were identified by physicochemical properties and spectral data. Result: Seven compounds were obtained from the ethyl acetate fraction of ethanol extract in the branches and leaves of S. gaussenii and identified as(7S, 8R)-dihydro-3-hydroxy-8-hydroxy-methyl-7-(4-hydroxy-3-methoxyphenyl)-1-benzofuranpropanol(1), (8R, 8'S, 7S)-4'-(3″-methoxyrhamnopyranosyl)oxy-8'-hydroxy-3, 3', 4-trimethoxy-8-hydroxymethyl-lign-7-9'-lactone(2), 7S, 8R-erythro-4, 7, 9, 9'-tetrahydroxy-3, 3'-dimethoxy-8-O-4'-neolignan(3), 7S, 8S-threo-4, 7, 9, 9'-tetrahydroxy-3, 3'-dimethoxy-8-O-4'-neolignan(4), leucoseceptoside A(5), coke to yellow benzene glycosides D(6), and blumenol A(7). Conclusion: Compounds 1-7 were all obtained from S. gaussenii for the first time.关键词：Cupressaceae;Sabina gaussenii;chemical constituents;lignans
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发布时间：2024-01-04 - 摘要：Objective: To conduct a global analysis on the infrared spectrum of Chebulae Fructus raw material, water extract, absolute ethanol extract, petroleum ether extract and the main component tannin. Method: Fourier transform infrared spectroscopy (FTIR), second-derivative infrared spectroscopy and two-dimensional correlation infrared spectrum (2D-IR) were adopted. Result: Chebulae Fructus raw material and extracts had respective stable spectral characteristics. The infrared spectrum of α, β-unsaturated ester bonds at 1 714 cm-1, and aromatic skeleton vibration at 1 613, 1 447 cm-1 were the main basis to determine the characteristic peak intensity of tannin in different Chebulae Fructus samples. After extraction, the main component tannin was effectively enriched. The characteristic peak of tannin was stronger in the three extracts, and the intensity of characteristic peak of tannin in water extract was higher than that in absolute ethanol extract, petroleum ether extract. Conclusion:The infrared spectrum macroscopical fingerprint technology could provide large amount of information on the overall structure, and provide reference for the extraction and separation of the main effective ingredients and accurately controlling the overall quality of Chebulae Fructus.关键词：Chebulae Fructus;tannic;extract;FTIR spectra
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining the contents of quercetin, caffeic acid, luteolin, and acacetin in Uygur Medicine Hyssopus cuspidatus. Method: Agilent C18 chromatographic column (4.6 mm×250 mm, 5 μm) was used with methanol-0.2% acetic acid solution as the mobile phase. The detection wavelength was set at 340 nm and the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the injection volume was 10 μL. Result: Quercetin, caffeic acid, luteolin, and acacetin showed good linear relationship in 0.120 2-1.081 8 mg(r=0.998 6), 0.031-0.279 mg(r=0.999 9), 0.052-0.468 mg(r=0.998 9), and 0.098 1-0.882 9 mg(r=0.998 8) respectively, and their recovery rate was 98.62%(RSD 1.4%), 97.38%(RSD 1.7%), 99.41%(RSD 1.5%), and 99.81%(RSD 1.0%) respectively. Conclusion: This RP-HPLC method is feasible and simple for simultaneous determination of 4 components in H. cuspidatus, which can provide theoretical indicators for the content determination of H. cuspidatus.关键词：Hyssopus cuspidatus;RP-HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining the content of songorine (a kind of diterpenoid alkaloids) in Aconiti Kusnezoffii Radix. Method: RP-HPLC was carried out on an Agilent Extend-C18(4.6 mm×250 mm, 5 μm) with acetonitrile-0.1% triethylamine solution (40: 60) as the mobile phase at a flow rate of 1.0 mL·min-1 and column temperature of 25℃. The drift tube temperature of ELSD was set at 96℃, with the gas (air) pressure of 2.50 bar, and the impactor was in a closed status. Result: The songorine showed good linear relationship in the range of 1.5-30 μg (r=0.999 5). Its average recovery rate was 96.86% with RSD of 1.7%. Conclusion: This established method is simple, quick and accurate, which can be used to determine the content of songorine in Aconiti Kusnezoffii Radix and provide reference for quality evaluation of Aconiti Kusnezoffii Radix.关键词：Aconiti Kusnezoffii Radix;songorine;RP-HPLC-ELSD;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To establish a quantitative analysis of multi-components with a single-marker (QAMS) method for the determination of five kinds of water-soluble components in Aconiti Lateralis Radix Praepapata. Method: With hydrochloride dopamine as the reference, chromatographic separation was achieved on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm). The content in five kinds of water-soluble components of aconite was determined and elative correction factors were calculated with methanol-0.2% phosphoric acid water as the mobile phase with gradient elution, at column temperature of 30℃ and flow rate of 1.0 mL·min-1, with detection wavelength of 260 nm and 280 nm. External standard method and QAMS assay were used to respectively determine the contents in 10 batches of aconite medicinal materials and verify the accuracy and feasibility of QAMS. Result: QAMS was established for determining five kinds of water-soluble components of in Aconiti Lateralis Radix Praepapata, and 10 batches of aconite were measured. The difference between the calculated and measured values was small (RSD < 5%). Conclusion: QAMS for measure five kinds of water-soluble components in Aconiti Lateralis Radix Praepapata is simple, effective, accurate, and provides a reference for its subsequent evaluation study.关键词：Aconiti Lateralis Radix Praepapata;quantitative analysis of multi-components by single marker;norepinephrine salsoline;hydrochloride dopamine
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC-ELSD method for simultaneous determination of ginsenosides Rg1, Rb1, Rd and astragaloside Ⅳ in Yixin Xuezhikang capsules. Method: Shimadzu C18 (4.6 mm×150 mm, 5 μm) was used, with acetonitrile-water as the mobile phase for gradient elution. The column temperature was 35℃, with a flow rate of 1.0 mL·min-1. The temperature of ELSD drift tube was set at 60℃, temperature of atomizing chamber at 30℃ and the gas pressure was 23.0 psi. Result: Excellent chromatographic separation was achieved for four active compounds (ginsenosides Rg1, Rb1, Rd, and astragaloside Ⅳ) with good linearity (r≥0.999 1) within the measurement range between mass concentration and peak area. The average recovery rate was 99.60% (RSD 1.6%), 99.89% (RSD 2.8%), 98.13% (RSD 2.7%) and 98.36% (RSD 2.5%) respectively for these four compounds. Conclusion: The method is simple, accurate, reproducible, and can be used as the quality control method for Yixin Xuezhikang capsules.关键词：Yixin Xuezhikang capsules;HPLC-ELSD;Ginsenoside Rg1;ginsenoside Rb1;ginsenoside Rd;astragaloside Ⅳ
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发布时间：2024-01-04 - 摘要：Objective: To investigate the metabolites of aesculin and aesculetin in rat urine. Method: After single oral administration of aesculin and aesculetin to rats, urine was collected within 48 h and pretreated by solid-phase extraction method. Metabolites were determined by a HPLC-MSn method. Result: Five major metabolites were found and their chemical structures were elucidated as hydrolysate, reduzate, methylated metabolite and sulfated product, respectively. Conclusion: According to the rule of metabolism, aesculin is hydrolyzed as aesculetin which were further metabolized in vivo. The method can be used to identify the structures of active components responsible for pharmacological effects.关键词：aesculin;aesculetin;metabolites;HPLC-MSn
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of wogonin on inhibiting the proliferation, migration and invasion of human colorectal cancer cells (HCT116 and HT29 cell lines), and investigate its effect on tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (Ywhaz) expression levels. Method: HCT116 and HT29 cell lines were cultured in vitro, and then treated with wogonin of different concentrations (5, 10, 20, 40 μmol·L-1) for various periods. Another blank group was also set up. Then the cell counting kit(CCK-8) was used to detect the effect of wogonin on proliferation of colorectal cancer cells;Annexin V-FITC/PI double standard flow cytometry was used to detect the apoptosis of colorectal cancer cells;Transwell method was used to detect the cells migration and invasioncapacities after 24 h treatment;Fluorescense real-time quantitative PCR method was used to detect mRNA expression levels of Ywhaz after 24 h treatment with different concentrations of wogonin, and their protein expression level and phosphorylation level were detected by Western blot assay. Result: As compared with blank group, wogonin significantly inhibited the proliferation of colorectal cancer cell lines in a dose-dependent manner, and promoted apoptosis of colorectal cancer cell lines;the inhibitory effect on cells proliferation was significant by 20, 40 μmol·L-1 wogonin (P < 0.01). Moreover, different concentrations of wogonin (10, 20, 40 μmol·L-1) could significantly reduce the number of transmembrane tumor cells (P < 0.05, P < 0.01), down-regulate the protein and mRNA expression levels of Ywhaz and reduce the phosphorylation level of Ywhaz in HCT116 and HT29 cell lines (P < 0.05, P < 0.01). Conclusion: Wogonin can significantly inhibit the proliferation, migration and invasion of HCT116 and HT29 cell lines and induce apoptosis. Its anti-tumor mechanism may be associated with down-regulating the protein expression of Ywhaz and its phosphorylation level.关键词：wogonin;colorectal cancer cells;proliferation;invasion;apoptosis;migration;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of extract from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma(EGNC) on the smooth muscle 22α (SM22α) expressions of replicative senescence vascular smooth muscle cells and cytoskeleton-associated protein. Method: With human aortic smooth muscle cells as the research objects and the replicative senescence 9th generation cells as the aging model, this experiment was divided into youth group (5th generation cells), model group (9th generation cell), EGNC low dose group (100 mg·L-1), middle dose group (200 mg·L-1), and high dose group (400 mg·L-1), resveratrol group (10 μmol·L-1). The drug intervention time was 48 h. β-galactosidase staining method was used to calculate blue dye cell ratio. The cell cycle was analyzed by the flow cytometry. Immunofluorescent staining method was used to observe the morphological changes of SM22α;while the mRNA and protein expressions of SM22α were detected by qPCR and Western blot respectively. Result: Compared with the youth group, the cell size was increased and shape was irregular in model;number of cells in G0/G1 stage was increased and number of cells in S stage was reduced;number of blue-dyed cells in β-galactosidase staining method was reduced (P < 0.01), conforming to the characteristics of aging model;in addition, SM22α staining was fuzzy and bleak, and the fluorescence intensity was weakened significantly, with reduced mRNA and protein expressions of SM22α (P < 0.01). As compared with the model group, drug intervention effectively reduced the number of blue-dyed cells, reduced the number of cells in G0/G1, increased the number of cells in S stage, enhanced the fluorescence staining intensity of SM22α, and increased mRNA and protein expressions of SM22α, with statistically significant difference (P < 0.05, P < 0.01). Conclusion: The replicative senescence vascular smooth muscle cells can be used as aging research models. The morphology, gene and protein expressions of SM22α were changed obviously in the process of cell aging, and it may involve in the process of cell aging together with cytoskeleton. EGNC delayed the aging of vascular smooth muscle cells to a certain extent and had obvious intervention effect on SM22α, so it might delay the aging of vessels with the cytoskeleton indirectly.关键词：aging;vascular aging;cytoskeleton;SM22α;extract from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma
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发布时间：2024-01-04 - 摘要：Objective: To observe the inhibitory effect of Aitongping capsules on tumor growth and metastasis in H22 tumor bearing mice, and explore its mechanism. Method: The ICR mice were inoculated with hepatoma cell line H22 to establish H22 tumor-bearing mice models. The H22 tumor-bearing mice were randomly divided into model group, cisplatin group, Aitongping high and low dose groups. The mice in the model group received the same volume of normal saline by ig daily, mice in Aitongping high-dose and low-dose group received 36, 18 g·kg-1 Aitongping by ig daily, and the mice in cisplatin group received 2 mg·kg-1cisplatin by ip daily. All the groups received continuous administration for 7 d, and then the mice were sacrificed. The tumor was peeled off for weighing, and the tumor inhibition rate was calculated according to the formula. Protein expression of vascular endothelial growth factor (VEGF) in tumor tissues was detected by immunohistochemistry, gene and protein expressions of C-X-C chemokine ligand 12(CXCL12) and its receptor C-X-C chemokine receptor 4(CXCR4) in tumor tissues by using Real-time PCR and Western blot method. Result: Aitongping high and low dose groups significantly inhibited the growth of tumors in H22 tumor-bearing mice. Aitongping high dose group significantly decreased the protein expression of VEGF in the tumor tissues, and inhibited the gene and protein expressions of CXCR4 (P < 0.01). Conclusion: Aitongping capsules have a certain inhibitory effect on tumor growth and metastasis in H22 tumor-bearing mice, and its mechanism may be associated with reducing protein expression of VEGF in tumor tissues and interventing CXCL12/CXCR4 biological axis.关键词：Aitongping capsule;tumor metastasis;mechanism;C-X-C chemokine ligand 12;C-X-C chemokine receptor 4
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发布时间：2024-01-04 - 摘要：Objective: To investigate the treatment effect of Yiqi Jiedu Huoluo formula(YQJDHL) in rats with early diabetic nephropathy (DN) and observe its effect on transforining growth factor-β(TGF-β)/Smad pathways. Method: Totally 72 SPF healthy male SD rats were used to establish diabetes mellitus (DM) models by tail injection of streptozotocin (STZ). Then according to the blood sugar levels, the rats were randomly divided into model group, YQJDHL prevention group (2.4 g·kg-1·d-1), YQJDHL low dose group and high dose group (2.4, 7.2 g·kg-1·d-1), positive drug group (benazepril hydrochloride, 10 mg·kg-1·d-1), another normal group was also set up, n=12 in each group. In the YQJDHL preventive group, YQJDHL compound formula was immediately given after DM models were made. While in YQJDHL high and low dose groups and positive drug group, the medicine was given two weeks after the DM models were made, that was immediately after DN models were made. Four weeks after intragastrical administration of YQJDHL formula, 24 h urine of rats was collected to determine the urine trace albumin, and the blood was taken by abdominal aorta puncture to determine blood glucose in rats;renal tissue samples were collected to observe the renal pathological changes by htoxylin-eosin (HE) staining method;the effects of YQJDHL TGF-β1, Smad3/7 mRNA and protein expression levels in the kidney tissues of early DN rats were observed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay. Result: As compared with the normal group, the blood glucose and urine trace albumin were significantly increased in rats of model group;TGF-β1 and Smad3 expression levels were significantly increased;Smad7 expression level was significantly reduced (P < 0.01);and the renal pathological changes were obvious in pathological observation. QJDHL treatment groups and preventive group could reduce the blood glucose and urine trace albumin in rats, reduce TGF-β1 and Smad3 expression levels in kidney tissues, and increase Smad7 expression (P < 0.05, P < 0.01);in addition, renal pathological changes were significantly improved. Conclusion: The results showed that the YQJDHL formula had the treatment and prevention effect for the DN, and its mechanism of action may be associated with TGF-β/Smad signaling pathways.关键词：Yiqi Jiedu Huoluo formula;diabetic nephropathy;transforming growth factor-β1;Smad
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发布时间：2024-01-04 -
Flavone from Galii Veri Herba et Radix-induced Apoptosis of Acute Promyelocytic Leukemia Cells (NB4)
摘要：Objective: To study the effect of flavone from Galii Veri Herba et Radix (FGVL) against acute promyelocytic leukemia cell line NB4, in order to provide a new evidence for future clinical treatment for acute promyelocytic leukemia. Method: In this study, the growth of acute promyelocytic leukemia cell line NB4 at different dosages of FGVL was observed with trypan blue staining. Methyl thiazoly1 terazolium(MTT) assay was used to detect the proliferation inhibition of NB4 cells. Acridine orange ethidium bromide (AO/EB) staining was used to detect morphological changes in NB4 apoptosis. DNA agarose electrophoresis was adopted to test apoptosis. The expression of pro-and anti-apoptotic genes Bax and Bcl-2 were examined by reverse transoription-polymerase chain reaction(RT-PCR). Result: Compared with the blank group, FGVL (50, 100, 200 mg·L-1) could inhibit the proliferation of NB4 cells(P < 0.01);morphology analysis presented the characteristics of apoptosis, and DNA agarose electrophoresis showed the typical DNA 1adder in 24 h. Accoridng to RT-PCR experiments, compared with the blank group, FGVL could down-regulate the mRNA expression of Bcl-2 and up-regulate the mRNA expression of Bax in NB4 cells (P < 0.01), with the positive correlation with the dosage. Conclusion: FGVL can resist acute promyelocytic leukemia NB4 cell proliferation and induce apoptosis, and its mechanism is related to the expression of Bcl-2/Bax. It provides a new possible clinical chemotherapy for acute promyelocytic leukemia.关键词：flavone from Galii Veri Herba et Radix;NB4 cells;apoptosis;Bcl-2;Bax;acute promyelocytic leukemia- 2
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of iridoid glycoside extract from Corni Fructus (ECF) on mitochondrial oxygen consumption and related genes expression in 3T3-L1 mature adipocytes, and analyze the possible molecular mechanism. Method: With 3T3-L1 adipocytes as the study objects, Seahorse XF Cell Mito Stress Test was used to detect the effect of ECF (at the concentrations of 0.04, 0.2, 1 g·L-1) for 2 d on the oxygen consumption rate in adipocytes, and blank group was additionally set up. Peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α), silent information regulator 2 related enzyme 1 (SIRT1), adenosine monophosphate activated protein kinase (AMPK), mitochondrial uncoupling protein 1 antibodies (UCP-1), PR domain proteins 16 (PRDM16) and peroxisome proliferators-activated receptor γ (PPARγ) mRNA expressions were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: As compared with the blank group, 2 days' intervention of 0.2, 1 g·L-1 ECF in mature 3T3-L1 adipocytes enhanced ATP production, basal respiration and maximal respiration;1 g·L-1 ECF intervention enhanced the spare respiration capacity;0.04 g·L-1 ECF significantly increased the oxygen consumption rate of proton leak. ECF intervention for 2 days at various concentrations significantly up-regulated PGC-1α, AMPK and SIRT1 mRNA levels, and significantly down-regulated PPARγ mRNA expression level, with statistically significant differences (P < 0.05, P < 0.01), but they showed no significant effects on UCP-1 and PRDM16 mRNA expression levels. Conclusion: In mature adipocytes, ECO could enhance the aerobic respiration and increase ATP production, and the adipocytes have no beiging characteristics, so these effects might be related to regulations of AMPK, SIRT1, PGC-1α and PPARγ levels.关键词：extract from Corni Fructus;iridoid glycoside;mature adipocyte;oxygen consumption rate;proton leak
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of apoptosis induced by chelerythrine (CHE) in human hepatocellular carcinoma cell line HepG2 in vitro. Method: With HepG2 cells as the study objects, the cell proliferation effect of CHE was detected by methyl thiazolyl tetrazolium (MTT) colorimetric method, the effect of CHE on apoptotic morphology changes in HepG2 cells was observed by Hoechst 33258 staining, the expressions of X protein related to Bcl-2 (Bax), anti-apoptotic protein (Bcl-xl) in B lymphocyte tumor-2 genes (Bcl-2) family, and pro-apoptotic protein Caspase-3 in cysteine family were detected by Western blot. Detect Bax, Bcl-xl, Caspase-3 mRNA expression levels of influence of CHE by Real-time PCR. Result: Compared with control group, CHE could significantly inhibit the proliferation of HepG2 cells. The 50% inhibitory concentration (IC50)of 6, 12, 24 h was 12.98, 10.53, 11.21 μmol·L-1 respectively. In Hoechst 33258 staining results, CHE group showed typical apoptosis characteristics. CHE in high concentration could up-regulate the protein and mRNA expression levels of Bax, Caspase-3, and decrease the protein and mRNA expression levels of Bcl-xl. As compared with the control group, there was statistically significant difference in above indexes (P < 0.05). Conclusion: Chelerythrine can inhibit the proliferation of HepG2 cells, and induce apoptosis by increasing the pro-apoptotic protein and mRNA expression levels and decreasing anti-apoptotic proteins and mRNA expression levels.关键词：chelerythrine;cancer line HepG2;apoptosis;mechanism;Bcl-2 family;Caspase-3
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发布时间：2024-01-04 - 摘要：Objective: To explore the pro-apoptosis effects of Qiyu Sanlong decoction (QYSL) and its biological immunoregulation effect on Helper T cells in mice with lung cancer. Method: The mice models of lung cancer were established in C57BL/6 mice using lewis lung carcinoma cells line (LLC) by culture and transplantation method. After successful modeling, the mice were randomly divided into model group, chemotherapy group, QYSL group and combination group, 8 cases in each group, another 8 cases normal mice were used as blank group. The survival state of mice was observed, the tumor was weighed and tumor inhibition rate was calculated. Transmission electron microscope was used to observe the ultrastructural changes of tumor cells and determine whether QYSL can induce apoptosis of tumor cells. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of interleukin (IL)-2, interferon (IFN)-γ, IL-4 and IL-10 in the spleen of mice. The transcriptional level of Bcl-2 and Bax mRNA expressions in tumor tissues was detected by Real-time PCR assay. Result: The survival state of QYSL group and combination group was significantly superior to that of chemotherapy group. As compared with the model group, tumor growth could be significantly inhibited by QYSL group, chemotherapy group and combination group, with significant differences (P < 0.01). Under electron microscope, the tumor cells change was mainly based on apoptosis and necrosis was also present in QYSL group, multiple apoptotic bodies were visible, typical necrotic cells were visible in combination group. Expression level of Bcl-2 mRNA in various treatment group was significantly lower than that in model group (P < 0.01). Bcl-2 mRNA expression level in QYSL group and combination group was lower than that in chemotherapy group (P < 0.01). Expression of Bax mRNA in treatment groups was significantly higher than that in model group, and the expression of Bax mRNA in combination group was increased as compared with the chemotherapy group. As compared with the model group and chemotherapy group, the IL-2 level in QYSL group and IFN-γ level in combination group were increased (P < 0.01). The IL-4 and IL-10 levels in QYSL group and combination group were lower than those in model group (P < 0.05), while above two levels in combination group were significantly lower than those in chemotherapy group (P < 0.01). Conclusion: Qiyu Sanlong decoction can improve the survival state and induce apoptosis of lung cancer cells in mice with lung cancer, and the apoptosis mechanism may be related to the down-regulation of Bcl-2 level and up-regulation of Bax level. Qiyu Sanlong decoction can also improve the immune function and inhibit the tumor immune escape by increasing IL-2, IFN-γ levels and decreasing IL-4, IL-10 levels to balance Th1/Th2 drift.
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Sanzi Yangqin Tang drug serum on cysteinyl leukotrienes (CysLTs)-mediated inflammatory pathways in bronchial epithelial cells. Method: Serum pharmacology technique was adopted to treat interleukin-4 (IL-4) and CysLTs-induced bronchial epithelial cells with 1.25%, 2.5% and 5% Sanzi Yangqin Tang drug serum as well as blank serum. The concentrations of eotaxin-1 (Eot-1) and Eot-3 in the culture supernatant were detected by enzyme linked immunosorbent assay (ELISA);the expression levels of cysteinyl leukotrienes receptor 1 (CysLTR1) and CysLTR2 in bronchial epithelial cells were detected by Western blot assay;the mRNA expression levels of CysLTR1 and CysLTR2 in bronchial epithelial cells were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: The concentrations of Eot-1 and Eot-3 in the culture supernatant in 2.50% and 5.00% Sanzi Yangqin Tang drug serum were significantly lower than those in the same serum levels of blank group (P < 0.05);there were no significant differences in protein expression level of CysLTR2 and mRNA expression levels of CysLTR1 between the culture supernatant of 1.25%, 2.50% and 5.00% Sanzi Yangqin Tang drug serum and the blank group;the protein expression level of CysLTR1 and mRNA expression level of CysLTR2 in the culture supernatant of 2.50% and 5.00% Sanzi Yangqin Tang drug serum were significantly lower than those in same serum levels of blank group (P < 0.05). Conclusion: Sanzi Yangqin Tang drug serum could block CysLTs-mediated inflammatory pathways and thus treat bronchial asthma by down-regulating the expression levels of CysLTR, as well as the expression and secretion of Eot-1 and Eot-3.关键词：Sanzi Yangqin Tang;Drug Serum;bronchial epithelial cells;cysteinyl leukotrienes receptor 1
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Yangyin Yishen particle (YYP) on HPA axis in glucocorticoid (GC)-induced kidney Yin-deficiency rats. Method: Totally 60 Wistar rats were randomly divided into normal group, model group, YYP high-, medium-, low-dose groups (6.48, 3.24, 1.62 g·kg-1·d-1) and Liuwei Dihuang wan group (0.39 g·kg-1·d-1), with 10 rats in each group. Except for the normal group, all of rats in other groups were intraperitoneally injected with 50 mg·kg-1·d-1 hydrocortisone for 7 consecutive days to establish setted up the kidney Yin-deficiency rat model by intraperitoneal injection of 50 mg·kg-1·d-1 hydrocortisone for 7 consecutive days. The normal group and the model group rats were gave given distilled water (10 mL·kg-1·d-1) by intragastrically, the YYP high-, medium-, low-dose groups and Liuwei Dihuang wan group were gave given the corresponding drugs by gavage for 7 days. The general situations changes of in general situations, such as rats activity, body hair, tail, food intake, stool and weight, were observed. The bilateran adrenal glands were removaled. The adrenal glands' weight and weight index were measured. The plasma level of corticotropin releasing hormone (CRH), adre-nocorticotropic hormone (ACTH) and cortiso (CORT) were tested by enzyme-linked immuno sorbent assay(ELISA) method. The CRH mRNA of hypothalamus, the ACTH mRNA of pituitary gland, and the CORT mRNA of adrenal gland were checked detected by reverse transcriptasepolyerase chain reaction(RT-PCR). The adrenal histology of adrenal were was inspected by hematoxyin-eosin(HE) staining. Result: Compared with the normal group, the general condition of the model group were poorer, showing the weight lost significantly (P < 0.01), the weight of adrenal gland and adrenal gland index decreased remarkably (P < 0.01), the CRH, ACTH and CORT of plasma reduced (P < 0.01), the mRNA expressions of CRH, ACTH and CORT were down-regulated (P < 0.01), with severe adrenal lesions. Compared with the model group, the general situations and weight of adrenal gland, adrenal gland index, CRH, ACTH and CORT of the YYP high-, medium-, low-dose group and Liuwei Dihuang wan group rised (P < 0.05, P < 0.01), adrenal glands' weight and weight index, the mRNA of CRH, ACTH and CORT were up-regulated (P < 0.01), and CRH, ACTH and CORT mRNA expressions were up-regulated remarkably, and the lesions degree of adrenal reduced. Conclusion: The Yangyin Yishen particle has the antagonistic effect against the inhibited HPA axis for kidney Yin-deficiency rat induced by GC.关键词：nourishing kidney Yin;glucocorticoid;kidney yin-deficiency syndrome;hypothalamus-pituitary-adrenal cortex axis
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发布时间：2024-01-04 - 摘要：Objective: To compare the protective effect of tanshinol and salvianolic acid B on glutamate-induced excitotoxicity in PC12 cells. Method: With PC12 cells as the study objects, the cultured cells were divided into blank group, glutamic acid treatment group (model group), salvianolic acid B group, tanshinol group, and vitamin E group (20 μmol·L-1). The cells in all the other groups except blank group and model group were treated with additional glutamic acid for co-incubation for 24 h after pre-treatment with salvianolic acid B, tanshinol and vitamin E respectively for 1 h. The cell activity was detected by methyl thiazolyl tetrazolium (MTT) method;lactic dehydrogenase (LDH) method was used to detect the leakage rate of lactate dehydrogenase, and the content of intracellular reactive oxygen was detected by the flow cytometry. Result: As compared with the blank group, tanshinol and salvianolic acid B could significantly inhibit the glutamate-induced neurotoxicity in PC12 cells and prevent the leakage of LDH (P < 0.05), showing a dose-response relationship in 50, 100, 200 μmol·L-1 dose. At 100 μmol·L-1 concentration, tanshinol was slightly better than salvianolic acid B in inhibiting reactive oxygen species(ROS) accumulation in PC12 cells and reducing the relative fluorescence intensity (about 12.5%). At the same dose condition, tanshinol was superior to salvianolic acid B in the protective effect on PC12 cells. Conclusion: Under the selective research models and concentrations, both tanshinol and salvianolic acid B had the potential effects on protecting glutamate-induced injury in PC12 cells, and the protective effect of tanshinol was slightly better than that of salvianolic acid B.关键词：comparison of the effects;tanshinol;salvianolic acid B;PC12 cells
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发布时间：2024-01-04 - 摘要：Objective: To provide foundation for further exploring the differences in hypevplasiasuppressorgene(HSG) expressions before and after Chinese medicine prescription treatment for different traditional Chinese medicine (TCM) syndromes of chronic obstructive pulmonary disease (COPD), and provide objective standards for diagnosis of COPD TCM syndromes by detecting the expression of HSG in different TCM syndromes of COPD. Method: The 110 patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and 30 normal people were randomly selected as the study subjects. They were divided into normal group, Qi deficiency group, phlegm heat group, and phlegm turbidity group. Blood was taken in fasting state in all inductees. The kit method was used to extract total RNA from the blood, mRNA expressions of HSG under different groups of AECOPD patients was detected by Real-time PCR, the protein expression of HSG under different groups of AECOPD patients was detected by Western bolt. Result: There was significant difference between normal group and phlegm turbidity group, phlegm heat group, Qi deficiency group. Conclusion: The expression levels of HSG were low in AECOPD patients and high in normal group. Expression levels of HSG in blood of Qi deficiency group were lower than those in phlegm turbidity group and phlegm heat group. HSG and COPD development may have an inherent correlation and HSG expression levels may affect airway reconstruction. It may be involved in the formation of different TCM syndromes in COPD.关键词：hypevplasiasuppressorgene;chronic obstructive pulmonary disease;traditional Chinese medicine syndrome;Real-time PCR;Western blot
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Yiqi Huoxue decoction on National Institute of Health stroke scale (NIHSS), traditional Chinese medicine (TCM) syndromes and heat shock protein-70 (HSP-70) scores of acute cerebral watershed infarction patients at several time points, and explore the correlation between dynamic changes of HSP-70 and disease development as well as evolution of TCM syndromes. Method: The 137 patients within 72 h of cerebral watershed infarction were randomly divided into treatment group (69 cases) and control group (68 cases). Patients in treatment group orally took Yiqi Huoxue decoction on the basis of treatment in control group. NIHSS and TCM Syndrome scores were recorded while HSP-70 data were collected within 3 days and on day 7, day 14 after disease onset. Means were compared and correlation analysis was done. Result: The differential value of NIHSS scores before and after treatment in treatment group was higher than that in control group (P < 0.05). The differential values of Qi deficiency score and blood stasis score before and after treatment in the treatment group were significantly higher than those in control group (P < 0.01). Within 3 days to the day 7 after disease onset, the increased degree of HSP-70 in treatment group was higher than that in control group (P < 0.05). From day 7 to day 14 after onset, the decreased degree of HSP-70 in treatment group was higher than that in control group (P < 0.05). HSP-70 was negatively correlated with NIHSS score, Qi deficiency score and blood stasis score at 3 time points (P < 0.05). NIHSS score was significantly positively correlated with Qi deficiency score and blood stasis score within 3 days and on day 7 after onset (P < 0.01). NIHSS score was positively correlated with Qi deficiency score (P < 0.05) and significantly positively correlated with blood stasis (P < 0.01) on day 14 after onset. Conclusion: Yiqi Huoxue decoction can help to improve the neurologic deficits, and significantly improve Qi deficiency and blood stasis of the patients with acute cerebral watershed infarction. Yiqi Huoxue decoction possibly beneficial to the cells protection effect of HSP-70. Because of the high correlation, the difference and relevance of HSP-70 expression level at different time points after onset can reflect the change rule of neurologic deficits and the evolution trend of TCM syndromes in patients with acute cerebral watershed infarction from micro perspective.关键词：cerebral watershed infarction;traditional Chinese medicine syndromes;heat shock protein-70;Yiqi Huoxue decotion
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Qingying Huoxue decoction in treating primary biliary cirrhosis (PBC) with liver-gallbladder dampness-heat syndrome, and investigate its effect on Th17/Treg levels in serum. Method: Ninety-eight PBC patients with liver-gallbladder dampness-heat syndrome were randomly divided into control group (49 cases) and treatment group (49 cases) by digital table method. The patients of control group received compound glycyrrhizin tablets by oral administration for two weeks (2 tablets/time, tid) and then ursodeoxycholic acid capsules were taken by oral administration after meals, 13-15 mg·kg-1·d-1, tid. The patients of treatment group received Qingying Huoxue decoction based on the treatment of control group (1 dose/d, bid). The treatment course was 24 weeks for both groups. The traditional Chinese medicine(TCM) clinical symptom scores were compared between two groups;the clinical efficacy of two groups after 24 weeks of treatment were analyzed. Helper T lymphocytes (Th17) and regulatory T cells (Treg) in peripheral blood were compared between two groups. The levels of interleukin(IL)-17, IL-6 and transforming growth factor-β(TGF-β) in the serum were detected in two groups. Result: The TCM clinical symptom scores in treatment group were significantly lower than those in control group after treatment (P < 0.01). The total response rate was 93.88% in treatment group, significantly higher than 77.55% in the control group (P < 0.05). Th17 level in peripheral blood of treatment group was significantly lower than that of control group after treatment, while Treg level was significantly higher than that of control group after treatment (P < 0.01). The levels of IL-17 and IL-6 in serum of treatment group were significantly lower than those of control group, while TGF-β level was significantly higher than that of control group after treatment, with statistically significant differences (P < 0.01). Conclusion: Based on the western medicine, Qingying Huoxue decoction could significantly improve clinical symptoms and clinical efficacy in the treatment of PBC with liver-gallbladder dampness-heat syndrome, and its mechanism may be related to regulating Th17/Treg balance in peripheral blood and its cytokine levels.关键词：Qingying Huoxue decoction;primary biliary cirrhosis;liver-gallbladder dampness-heat syndrome;helper T lymphocytes;regulatory T cells
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Mahuang Lianqiao Chixiaodou Tang for allergic rhinitis (AR) and investigate its effect on interferon-γ (IFN-γ), interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-17 (IL-17), interleukin-10 (IL-10) and total IgE(TIgE) levels in serum of the patients. Method: One hundred and twenty-five patients with AR were randomly divided into control group (62 cases) and observation group (63 cases) by random number table, and another 20 cases were used as healthy control group. Patients in control group received budesonide aerosol, 200-1 600 μg/day in 2-4 times. Patients in observation group orally took Mahuang Lianqiao Chixiaodou Tang, 10 g/time, 2 times/day. The treatment course was 7 days for both groups. Before and after treatment, levels of IFN-γ, IL-2, IL-4, IL-5, IL-17, IL-10 and TIgE levels were detected. Scores of nasal obstruction, rhinocnesmus, sternutation and rhinorrhoea as well as the overall symptoms were graded by visual analogue scale (VAS) both before and after treatment. Result: The clinical cure rate was 80.95% in the observation group, higher than 61.29% in the control group according to chi square test (P < 0.05). Total clinical effective rate was 95.24% and 91.94% respectively in observation group and control group, and there was no statistically significant difference between two groups according to chi square test. After treatment, the scores of nasal obstruction, rhinocnesmus and sternutation in observation group were lower than those in control group (P < 0.05). Levels of IFN-γ, IL-2 and IL-10 in serum of two treatment groups were lower than those in healthy control group (P < 0.01), while the levels of IL-4, IL-5, IL-17 and TIgE were higher than those in healthy control group (P < 0.01). As compared with the data before treatment, the levels of IL-2 and IL-10 in serum were significantly increased in both groups (P < 0.01), while the levels of IL-5, IL-17 and TIgE were significantly decreased (P < 0.01). There was no statistically significant difference in IFN-γ level change in both groups. After treatment, the levels of IL-2 and IL-10 in serum of observation group were higher than those in control group, while the levels of IL-5, IL-17 and TIgE were lower than those in control group (P < 0.01). There was no statistically significant difference in IFN-γ and IL-4 between two groups after treatment. The levels of IFN-γ, IL-2 and IL-10 in the two treatment groups were still lower than those in healthy control group after treatment (P < 0.01), while the levels of IL-4, IL-5, IL-17 and TIgE were still higher than those in healthy control group (P < 0.01). Conclusion: Mahuang Lianqiao Chixiaodou Tang can regulate the Th1/Th2, Th17 and Treg levels in patients with allergic rhinitis, promote the recovery of balance and relieve inflammatory response, and its clinical efficacy was superior to budesonide aerosol for allergic rhinitis.关键词：allergic rhinitis;Mahuang Lianqiao Chixiaodou Tang;interleukin-17;interleukin-10
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Guishen pills for premature ovarian failure(POF) with syndrome of kidney-yin deficiency and observe its effect on levels of growth differentiation factor-9 (GDF-9) in serum and bone morphogenetic protein-15(BMP-15). Method: One hundred and sixteen POF patients were randomly divided into control group (58 cases) and comprehensive group (58 cases) by random number table. The patients of control group received conjugated estrogen tablets for 20 days (1 tablet/time, qd);medroxyprogesterone acetate tablets were added from day 16 to day 20 (4 tablets/time, qd). Based on the treatment of control group, patients in comprehensive group were also treated with Guishen pills (1 dose/d, bid, stop in menstrual period). 20 days were regarded as one treatment course, and the patients in both groups were treated for 3 courses. Traditional Chinese medicine(TCM) symptom scores, follicle-stimulating hormone (FSH), estradiol (E2) and luteinizing hormone(LH) levels in serum were compared between two groups. The levels of GDF-9 and BMP-15 in serum were detected in two groups. Result: TCM symptom scores in comprehensive group were significantly lower than those in control group after treatment (P < 0.01). The total clinical effective rate was 94.83% in comprehensive group, significantly higher than 81.03% in control group(P < 0.05). The levels of FSH and LH in serum of comprehensive group were significantly lower than those of control group after treatment, while E2 level was significantly higher than that of control group (P < 0.01). The levels of GDF-9 and BMP-15 in serum of comprehensive group were significantly higher than those of control group, with statistically significant differences (P < 0.01). Conclusion: On the basis of routine western medicine treatment, Guishen pills could obviously improve clinical symptoms, hormonal levels, and clinical efficacy. The mechanism may be associated with increasing GDF-9 and BMP-15 levels in serum.关键词：Guishen pills;premature ovarian failure;syndrome of kidney-yin deficiency;growth differentiation factor-9;bone morphogenetic protein-15
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Shiquan Dabu Tang combined with Wuwei Xiaodu Yin on immune function and living quality in postoperative Qi and blood deficiency patients with esophagus cancer. Method: Ninety eligible patients were selected in this study and randomly divided into control group (44 cases) and treatment group (46 cases). Patients in control group received enteral nutrition supporting therapy on postoperative day 2 to day 9. Patients in treatment group were treated with Shiquan Dabu Tang combined with Wuwei Xiaodu Yin based on the treatment in control group. Symptom scores of traditional Chinese medicine (TCM) were compared between two groups. Levels of serum CD4+, CD8+, IgA, IgG and IgM were detected in serum of two groups. Quality of Life Questionary-Core 30 (EORTC QLQ-C30) V3.0 scores and Esophageal Cancer Supplementary Schema Table (QLQ-OES18) scores were compared between two groups. Result: The TCM symptom scores in treatment group were significantly lower than those in control group on postoperative day 10(P < 0.01), CD4+, CD4+/CD8+, IgA, IgG and IgM levels in treatment group were significantly higher than those in control group on postoperative day 10, while CD8+ level was significantly lower than that in control group(P < 0.01), scores of functional area indexes in treatment group were significantly higher than those of control group, while scores of symptom area indexes in treatment group were significantly lower than those of control group(P < 0.01), on postoperative day 10, scores of EORTC QLQ-OES18 in treatment group were significantly lower than those of control group with statistical differences (P < 0.01). Conclusion: Based on enteral nutrition supporting therapy, Shiquan Dabu Tang combined with Wuwei Xiaodu Yin in treatment of postoperative esophagus cancer with deficiency of Qi and blood could improve TCM symptoms and quality of life, and increase immune function.关键词：Shiquan Dabu Tang;Wuwei Xiaodu Yin;esophagus cancer;deficiency of Qi and blood;CD4+cell;CD8+cell;Ig
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发布时间：2024-01-04 - 摘要：Objective: To investigate the value of combined application of compound Kushen injection and cisplatin intraperitoneal chemotherapy in patients with gastric cancer and malignant ascites. Method: The 96 patients with gastric cancer and malignant ascites in our hospital from January 2012 to December 2013 were selected and divided into study group and control group with random number table method, 48 cases in each group. Patients with gastric cancer and malignant ascites in study group were treated with compound Kushen injection combined with cisplatin intraperitoneal chemotherapy, while patients with gastric cancer and malignant ascites in control group were treated with cisplatin intraperitoneal chemotherapy only. The clinical treatment conditions of patients in two groups were compared and analyzed. Result: As compared with the control group, the ratio of partial remission and total effective rate were increased in the patients with gastric cancer in study group, with significant differences (P < 0.05), the improvement ratio of life quality was increased in the study group, with statistically significant differences (P < 0.05), the ratio of reduction was increased, with highly statistically significant difference (P < 0.01), NK cells and CD3+ cell were increased significantly, with statistically significant differences (P < 0.05), CD4+ cell and CD4+/CD8+ were increased significantly, while CD8+ cell was reduced significantly, with highly statistically significant differences(P < 0.01), the overall incidence of adverse reactions was significantly lower than that in control group, with highly statistically significant difference (P < 0.01). Conclusion: Compound Kushen injection combined with cisplatin intraperitoneal chemotherapy had extremely important practical significance in improving the clinical efficacy, improving quality of life, enhancing immunity, and reducing the adverse reactions for patients with gastric cancer and malignant ascites.关键词：gastric cancer;malignant ascites;compound Kushen injection;cisplatin;chemotherapy
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发布时间：2024-01-04 - 摘要：Objective: To investigate the prescription rules of Professor YANG Mu-xiang in treatment of chronic gastritis. Method: Based on the Traditional Chinese Medicine Inheritance Support System, some data mining methods without supervision, such as asocciation rules Apriori algoritlma, complex system entropy clustering, were used to analyze the use frequency of herbs in the prescriptions as well as the asocciation rules and prescription laws between various herbs, and discuss the prescription experience of professor YANG Mu-xiang in treatment of chronic gastritis. Result: Seven Chinese herbs with highest use frequency, common herb combinations and core herbs in Professor YANG Mu-xiang's prescriptions for chronic gastritis were obtained. Conclusion: Based on the exploration of veteran doctors' experience in traditional Chinese medicine treatment on the Traditional Chinese Medicine Inheritance Support System Platform, new knowledge and information except those in previous traditional medical records collation and statistical research was obtained in this study, which can provide valuable reference for the further exploring and inheritance of professor YANG Mu-xiang's treatment of chronic gastritis.关键词：data mining;YANG Mu-xiang;chronic gastritis;experience
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发布时间：2024-01-04 - 摘要：Objective: To systematically review the efficacy and safety of Kudiezi injection in the treatment of unstable angina pectoris. Method: VIP, CBM, CNKI, Wanfang and PubMed were searched to find the clinical randomized controlled trials on Kudiezi injection treatment for unstable angina. The literature were screened according to inclusion and exclusion criteria, and evaluated according to Jadad quality assessment criteria;Revman 5.3 software was used for Meta-analysis. Result: A total of 10 randomized controlled trials were included in the systematic review, all of which were low-quality studies with Jadad score < 3 points. Meta analysis showed that Kudiezi injection was better than the control group in total effective rate [OR=3.31, 95%CI(2.23, 4.92), P < 0.000 01], markedly effective rate [OR=1.67, 95%CI(1.28, 2.18), P=0.000 1] and electrocardiogram efficacy [OR=2.70, 95%CI(1.95, 3.74), P < 0.000 01] for the treatment of unstable angina pectoris, with statistically significant differences. Conclusion: Kudiezi injection in the treatment of unstable angina can significantly improve the total effective rate, markedly effective rate and electrocardiogram efficacy with satisfactory safety. However, the existing small-sample randomized controlled trials are of low methodological and reporting quality, so large-sample high-quality clinical studies are required for further verification.关键词：Kudiezi injection;unstable angina pectoris;randomized control trial;systematic review
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发布时间：2024-01-04 - 摘要：Objective: To systemically assess the clinical efficacy of compound Xuanju capsule for chronic prostatitis. Method: We systemically searched China National Knowledge Infrastructure (CNKI), VIP Database, PubMed and Cochrane Library for clinical trials. The information was recorded and the methodological quality of the included trials was assessed by two reviewers independently, and the valid data was analyzed by RevMan 5.2 software. Result: The 10 randomized control trials involving 1 506 patients were included in the study. Meta-analysis showed that there was statistically significant difference in clinical efficacy between compound Xuanju capsule plus antibiotics group and the antibiotics alone group in the treatment of chronic prostatitis [OR=2.86, 95%CI (2.28, 3.58), P < 0.000 01]. Conclusion: The current evidence showed that, the compound Xuanju capsule combined with antibiotics in the treatment of chronic prostatitis was more effective than antibiotics alone. However, large and high-quality clinical trials are still required for verification.关键词：compound Xuanju capsule;antibiotics;chronic prostatitis;systematic review;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical efficacy of Xiyanping combined with ribavirin in the treatment of children with hand-foot-mouth disease. Method: Pubmed, Embase, Cochrane library, CNKI, CBM, WanFang Data and VIP were searched for randomized clinical trials (RCT) on Xiyanping combined with ribavirin in the treatment of children with hand-foot-mouth disease. Two independent reviewers strictly evaluated the quality of the included studies and extracted the data, then Meta-analysis was done by using RevMan 5.3 software. Result: Eventually 62 RCT involving a total of 8 945 children were included in the study. Meta-analysis results showed that there was statistically significant difference in total effective rate, antifebrile time, herpes fading time, recovery time of erythra in hands and feet, healing time of oral ulcer, cure time and hospital stay between Xiyanping combined with ribavirin group and control group in the treatment of children with hand-foot-mouth disease (P < 0.05). Conclusion: Xiyanping combined with ribavirin had certain effect in the treatment of children with hand-foot-mouth disease, but the quality of existing studies is poor, so more rigorous high quality multi-center randomized double-blind clinical studies are required for verification.关键词：Xiyanping;ribavirin;children with hand-foot-mouth disease;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the studies and ideas on traditional Chinese medicine treatment for male and female infertility based on data mining in Qing dynasty Yulin Recipe Ropy. Method: The Chinese medicine compound recipes for male and female infertility in Qing Dynasty Yulin Recipe Ropy were input to the computer for establishment of a database, and then statistical methods were used to analyze the rule of medication. Result: Totally 117 compound Chinese medicine recipes involving 199 herbs and 964 medication times were collected;core herbs with high-frequency mainly included Moschus, Caryophylli Flos, Aconiti Lateralis Radix Praeparata, Cinnamomi Cortex, Cnidii Fructus, Pericarpium zanthoxyli, Bufonis Venenum, Epimedii Folium. The drugs mainly included the tonic drugs, interior-warming drugs, resuscitation-inducing drugs, and astringent drugs;the herbs with pungent, sweet, and bitter flavors had a cumulative frequency of 80.68%, the herbs with main nature of warming, flatness and coldness had a cumulative frequency of 87.87%, the herbs returning to kidney, spleen, liver, heart, lung, and stomach had a cumulative frequency of 91.39%. Conclusion: Moschus, Caryophylli Flos, Aconiti Lateralis Radix Praeparata, Cinnamomi Cortex, Cnidii Fructus, Pericarpium zanthoxyli, Bufonis Venenum, Epimedii Folium were used with high-frequency in clinical use as recorded in the ancient book;addition and reduction of warming drug and Yang supplementing drug were regarded as the medication characteristics for male and female infertility syndrome.关键词：Qing dynasty Yulin Recipe Ropy;male and female infertility;medicine thought
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发布时间：2024-01-04 - 摘要：Irritable bowel syndrome (IBS) is a common functional intestinal disease, with abdominal pain or discomfort associated with bowel habit change as the main clinical manifestation, and its etiology has not been fully clear yet. Brain-gut Peptides with dual functions of transmitters or modulators for hormones and nerves, such as corticotrophin releasing factor (CRF), substance P (SP), motilin (MTL), vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), nerve peptide Y (NPY) and somatostatin(SS), play an important role in the pathogenesis of IBS. In vivo brain-gut peptide levels in IBS patients can reflect their disease progression from a certain extent. Chinese medicine interventions can regulate the abnormal changes of brain-gut peptides, thereby improve the symptoms. This review was written based on the brain-gut peptide changes of IBS patients and experimental animals as well as the effects of traditional Chinese medicine interventions, to provide ideas for the research on traditional Chinese medicine prevention and treatment of IBS.关键词：irritable bowel syndrome;brain-gut peptide;traditional Chinese medicine
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发布时间：2024-01-04 - 摘要：Network formulaology has the features of combining network science and characteristics of traditional Chinese medicine(TCM) formulaology, with the help of modern technology, integrating macroTCM holistic thinking and modern molecular medicine microscopic biological perspective, through knowledge-associated network construction and analysis, to establish a systemicformula repository as well as disease and syndrome network, focusing on the integrated regulation mechanism of effective components in the TCM formulae, clarification of compatibility laws, new drug development and the analogous formulae networks. Material foundation research reveals the action mechanism of TCM formulae, so it is the guarantee of quality control and safety of TCM formulae, compatibility law is the core of TCM formulae, TCM formulae has advantages of integrated regulator in complex diseases treatment, so it is an important source to develop new Chinese herbal medicines. Research of analogous formulae can help to reveal the complex systems of TCM formulae and understand the core issues of compatibility law. The above four aspects are the focuses and difficulties in modernization research of TCM formulae, and also act as the important contents and directions of network formulaology. Because of the multi-component, multi-target and multi-channel complex mechanisms of TCM formulae, its scientific connotation has not been clarified yet. The development of network formulaology is beneficial to interpret the scientific connotation of TCM compounds, improve the efficacy of clinical medicine, and expand the ideas of new drugs. However, the scope ofresearch, related results and development directions of the network formulaology are lack of systematic teasing currently, not conducive to promoting modernization of formulaology. The research methods and progress on fundamental research, compatibility theory, new drug development, and analogous formulae research were reviewed in this paper, to clarify the prescription principles, compatibility law and clinical useof TCM formulaology.关键词：network formulaology;material foundation;compatibility law;development of Chinese herbal medicine;analogous formulae research
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发布时间：2024-01-04 - 摘要：Chinese medicine is the foundation of traditional Chinese medicine (TCM) to prevent and cure diseases, its authenticity will directly affect the safety and effectiveness of clinical medication. Standardization is the premise of the modernization of Chinese medicine, because it is a complex system, which has characteristics of multi-level and multi-target. And traditional method to identify Chinese medicine is mainly on the basis of character identification which is difficult to guarantee the objectivity and accuracy, and the standardized digitization in Chinese medicine has become the inevitable direction along with the development of it. And the modern development of digital research can only be a good study on the quality control of medicinal materials, but it can't control the efficacy of Chinese medicine. Standardized digitization of Chinese medicine in recent years were summarized in order to make a contribution to the development of digitization in Chinese medicine. At the same time, the author suggests that the future development of digitization in Chinese medicine should be combined with the character and determination.关键词：Chinese medicine;complex system;standardization;digitization;color
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发布时间：2024-01-04 - 摘要：There are four species from the genus Hosta in China, and they have been used as traditional folk medicines by Han, Mongolian, Thai, Naxi and many other nationalities for a long history. With the functions of clearing away heat and toxic materials, and relieving cough and sore throat, they were collected in Great Dictionary of Chinese Medicine, Chinese Materia Medica, Zhongguo Minzuyao Zhiyao, Chinese Materia Medica-Mongolian Medicine, Inner Mongolia Medicinal Standard and other books. The commercially available Mongolian medicines with flowers of Hosta plantaginea(Lam.) Aschers as the main medicinal material include Yuzan Qingyan Shiwuwei Wan, Yuzan Qingyan Shiwuwei San, and Qingyan Liuwei San, mainly used for swollen and sore throats, asthma, hoarseness, and sternocostal tingling. Comprehensive analysis of the literature from CNKI, Wanfang, Web of Science, SciFinder, Pubmed and other databases showed that steroidal compounds were the major and representative constituents in the plant of Hosta, displaying anti-inflammatory, anti-tumor, and antimicrobial activities. However, the medicinal effectiveness and action mechanisms for the above pharmacological actions are still unknown. The studies showed that steroidal saponins isolated from Chinese herbal medicine had anti-inflammatory effect relying on hypothalamic-pituitary-adrenal cortex axis, similar to the glucocorticoids. Therefore, study on the steroids from the genus Hosta and their anti-inflammatory activities is very import for elucidating the medicinal effectiveness and mechanism of anti-inflammatory effect of genus Hosta. However, there are few studies on the chemical constituents and pharmacological activities of genus Hosta in China. This review aims to provide reference for the quality control, clinical application and reasonable development of Hosta plants by sorting, analyzing and summarizing the researches both at home and abroad on steroids constituents from genus Hosta and their biological activities.关键词：Hosta;steroids;chemical constituents;pharmacological activities
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