最新刊期
卷 22 ， 期 3 ， 2016
- 摘要：Objective: To establish an artificial neural network for predicting retention rate of total polysaccharides in fibrous rhizome herbs by ultrafiltration technology. Method: Taking pore size of inorganic ceramic membrane,pressure and temperature as input parameters,retention rate of total polysaccharides in enzymatic hydrolysate of Hedysari Radix under different ultrafiltration conditions as output parameter,BP neural network was established after network parameters optimized by Levenberg-Marquardt method.Then performance and applicability of model were evaluated. Result: Topological structure of BP neural network was 3-6-1.Mean prediction error,mean absolute error and mean error rate of BP neural network for retention rate of total polysaccharides in Hedysari Radix was 0.10%,0.98% and 1.55%,respectively.Mean error rate for retention rate of total polysaccharides in Astragali Radix was 2.77%. Conclusion: Accuracy and applicability of BP neural network is good enough to predict retention rate of total polysaccharides in fibrous rhizome herbs by ultrafiltration technology.关键词：Hedysari Radix;Astragali Radix;total polysaccharides;ultrafiltration;prediction model;BP neural network
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发布时间：2024-01-04 - 摘要：Objective: To investigate rationality of vacuum-assisted extraction (VAE) technology for Carthami Flos and optimize its extraction conditions. Method: Response surface methodology was used to optimize VAE technology of Carthami Flos with yield of hydroxy safflower yellow A as index,its major factors were boiling temperature,ethanol concentration,extraction time and cycles,which had influence on yield of hydroxy safflower yellow A were tested by an Box-Behnken design.Besides,micro-morphology of plant materials were observed by scanning electron microscope (SEM). Result: Optimum extraction conditions were as follows:extracted twice with 20 times the amount of 50% ethanol at 65℃,extraction time of 42 min for per time.Under these conditions,yield of hydroxy safflower yellow A reached 1.51%. Conclusion: Compared with conventional heat reflux extraction,VAE has higher extraction efficiency.VAE is an efficient and simple method for extracting hydroxy safflower yellow A from Carthami Flos,which shows great potential for becoming an alternative technique for extraction of heat sensitive compounds from traditional Chinese medicine.关键词：Carthami Flos;hydroxy safflower yellow A;vacuum-assist extraction;scanning electron microscope;response surface analysis
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发布时间：2024-01-04 -
Optimization of Extraction Process and Pharmaceutical Formulation of Wuwei Jinse Dispersible Tablets
摘要：Objective: To optimize extraction process and pharmaceutical formulation of Wuwei Jinse dispersible tablets. Method: Taking composite score of contents of total polyphenols and gallic acid,dry extract rate as index,orthogonal test was adopted to investigate influence of extraction time,extraction times,the amount of water on water extraction process.With appearance,hardness and dispersion uniformity time as indexes,molding process of dispersible tablets was optimized by single factor tests. Result: Optimum extraction process consisted of adding 10-fold of waters,extracting 3 times,90 min for each time.Yield of total polyphenols was 6.29%,the content of gallic acid was 398.37 mg,dry extract rate was 24.28%.This dispersible tablets were made up of amylum pregelatinisatum,microcrystalline cellulose,croscarmellose sodium and micropowder silica gel,and it can be disintegrated completely and dispersed uniformly within 130 s. Conclusion: Optimized extracting process and pharmaceutical formulation are feasible and stable for industrial production of Wuwei Jinse dispersible tablets.关键词：Wuwei Jinse pills;dispersible tablets;gallic acid;total polyphenols;information entropy- 2
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发布时间：2024-01-04 - 摘要：Objective: To improve quality of water extract of Angelicae Sinensis Radix and reduce production cost by investigating feasibility of purification and concentration process of water extract using ultrafiltration and nanofiltration integrated technology. Method: With retention rate of ferulic acid as index,ceramic membrane pore size,working pressure,filtration temperature as factors,orthogonal test was adopted to optimize ultrafiltration process of water extract of Angelicae Sinensis Radix.With leak rate of ferulic acid as index,interception relative molecular weight and solution temperature as factors,concentration process of ultrafiltrate was optimized by comprehensive test. Result: Optimum ultrafiltration process parameters were as follows:membrane pore size of 50 nm,filtration pressure at 0.10 MPa,filtration temperature at 50℃;retention rate of ferulic acid was more than 90%.Optimum nanofiltration process parameters were as follows:interception relative molecular weight of 200 Da,filtration temperature at 45℃;leak rate of ferulic acid was 0.1%. Conclusion: Ultrafiltration and nanofiltration integrated technology has many advantages,such as retention rate of active ingredients is high and material liquid is heated in a short time,it can improve quality of water extract of Angelicae Sinensis Radix with low production cost.关键词：Angelicae Sinensis Radix;water extract;ultrafiltration process;nanofiltration;ferulic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology of oligosaccharides in Morindae Officinalis Radix by macroporous resin and prepare effective parts of oligosaccharides with high purity. Method: Taking adsorption-desorption rate of nystose and oligosaccharides as indexes,effects of the concentration of sample solution,diameter-height ratio,elution agent and other factors on purification technology of oligosaccharides were investigated by single factor tests. Result: Optimum purification technology was as following:the concentration of sample solution 40 g·L-1,diameter-height ratio of resin column 1:6,ratio of sample volume-resin 1:25,then eluted with 12 BV of water with flow rate of 2 BV·h-1,collected eluent.Average content,adsorption-elution rate and transfer rate of oligosaccharides in water eluent were 55.70%,94.95%,91.82%,respectively. Conclusion: This optimized purification technology is simple and stable with low cost,purity of oligosaccharides is more than 50%.关键词：macroporous absorption resin;purification process;nystose;Morindae Officinalis Radix;oligosaccharides
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发布时间：2024-01-04 - 摘要：Objective: To investigate inhibition of baicalin on activity of cytochrome P450 (CYP450) enzyme in rat and human liver microsomes, and then compare species difference between them.In addition,possibility of drug interaction with baicalin was evaluated. Method: Liver microsomes combine with specific probe substrates were used in vitro and metabolites of substrates were determined by LC-MS/MS.The mass spectrometer was operated with electrospray ionization (ESI) and selective reaction monitoring (SRM) scan mode.Chromatographic separation was performed on a ZORBAX Eclipse-plus C18 column with mobile phase of methanol-water (containing 0.1% formic acid) by gradient elution.Hemi-inhibitory concentration (IC50) was calculated to assess inhibition of baicalin on CYP450. Result: In rat liver microsomes,baicalin showed no inhibition on CYP1A2,CYP2B6,CYP2C9,CYP2C19,CYP2D6,CYP2E1 and CYP3A4.However,in human liver microsomes,CYP1A2,CYP2C19 and CYP2E1 were inhibited weakly by baicalin,IC50 of them were 39.72,40.91,32.83 μmol·L-1. Conclusion: Species difference exists in inhibition of baicalin on CYP450,baicalin is a weak inhibitor for CYP1A2,CYP2C19 and CYP2E1.Drug interaction caused by inhibition of CYP450 enzymes should be paid attention,when baicalin is used intravenously in clinic.关键词：baicalin;cytochrome P450 enzyme;inhibition;drug interaction
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发布时间：2024-01-04 - 摘要：Objective: To provide basis for utilization and development of Rhei Radix et Rhizoma through comparing its quality from different areas in Henan. Method: Rhapontin was checked,qualitative identification was determined by TLC,HPLC was employed to determine the content of free anthraquinones with mobile phase of methanol -0.1% phosphoric acid (85:15) and detection wavelength at 254 nm. Result: In 9 batches of samples,4 batches of Rhei Radix et Rhizoma contained rhapontin,it was identified as Rheum franzenbachii;5 batches did not contain rhapontin,the content of free anthraquinones all exceed 1.5% and conformed to rules in the 2010 edition of Chinese Pharmacopoeia,the content of free anthraquinones (4.65%) was higher in samples from Lushan county,followed by samples from Song county with 4.38%,These two kinds of samples were from R.officinale and R. palmatum,respectively. Conclusion: R. officinale and R. palmatum from different areas in Henan are all consistent with rules in the 2010 edition of Chinese Pharmacopoeia,it is worthy of exploitation and utilization.R. franzenbachii cannot be applied in medicinal materials as Rhei Radix et Rhizoma.关键词：Rheum officinale;R. palmatum;R. franzenbachii;aloe-emodin;physcion;rhapontin
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发布时间：2024-01-04 - 摘要：Objective: To establish methods for determining residual of imidacloprid and spinosad in Salviae Miltiorrhizae Radix et Rhizoma,and investigate dynamic residual of these two pesticides. Method: Samples were extracted with organic solvents,purified by solid phase extraction cartridges and detected by HPLC-DAD. Result: These two pesticides were pretreated by solid phase extraction (SPE) method,and purified more thoroughly.Detection methods had high sensitivity,good reproducibility,accuracy,precision and detection limits were in line with requirements of determination of pesticide residues.Degradation dynamics data showed that considering residues of 2 h as initial residues, half-life periods (t1/2) of imidacloprid and spinosad were 3.65 d and 1.64 d.It showed that spinosad degraded faster than imidacloprid in the beginning.After one day,degradation curves of imidacloprid and spinosad in Salviae Miltiorrhizae Radix et Rhizoma consistented with first-order kinetic equations,t1/2 of them were 6.93 d and 11.55 d.It showed that spinosad had a longer duration of pesticide.At the same dosage level of pesticide,residue of spinosad was lower than imidacloprid,it showed that safe periods of spinosad was shorter than imidacloprid.The final residues of imidacloprid in soil and product were 0.02 mg·kg-1 and 0.18 mg·kg-1,and spinosad was not detected (<1.9 μg·kg-1) which showed that the final residue of spinosad was lower. Conclusion: It is concluded that in Salviae Miltiorrhizae Radix et Rhizoma planting,spinosad can be an ideal alternative pesticide to imidacloprid.关键词：Salviae Miltiorrhizae Radix et Rhizoma;imidacloprid;spinosad;half-life;pesticide residues
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发布时间：2024-01-04 - 摘要：Objective: Endophytes, as a kind of good biological resources, have already produced many metabolites with new structures and various activities. In this paper, during the study of medicinal plant's endophytes, the author isolated and obtained Talaromyceson fungi which could produce diverse metabolites. This paper is to isolate and identify cyclopeptides from this endophytic fungus and study on their acetylcholineterase inhibitory activity. Method: The compounds were isolated by column chromatography methods, including silica gel column chromatography, punching resin column chromatography, and MCI gel chromatography;the structures of compounds were identified using wave spectrum methods, including hydrogen nuclear magnetic resonance (NMR), carbon nuclear magnetic resonance and electrospray mass spectrometry;acetylcholineterase inhibitory activity of the compounds was detected using improved Ellman method. Result: Four cyclopeptides were isolated and identified as discarine-M(1), fumitremorgin C(2), fructigenine B(3), spirotryprostatin A(4). Compound 1 showed moderate anti-acetylcholineterase activity with IC50 value of 56 μmol·L-1. Conclusion: All of the four cyclopeptides were isolated and obtained from Talaromyces genus for the first time.关键词：cyclopeptides;Talaromyces;Tripterygium wilfordii
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发布时间：2024-01-04 - 摘要：Objective: To systematically study the chemical compositions of Rodgersia aesculifolia from Taibai Mountains, and provide theoretical basis for its further pharmacological research. Method: The compounds from R. aesculifolia were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography and recrystallization method. Their structures were identified according to their physicochemical properties and spectra analysis. A minimal inhibitory concentration (MIC) method was used in antibacterial activity test for the compounds. Result: Eight compounds were isolated and identified as β-sitosterol(1), bergenin(2), catechin(3), p-hydroxybenzoic acid(4), 3β-hydroxyolean-12-en-27-oic acid(5), gallic acid(6), succinic acid(7), and methylmalic acid(8). Conclusion: Compounds 4, 5, 7 and 8 were isolated from the plant of R. aesculifolia for the first time and compound 5 was first obtained from the genus. Compound 5 showed good inhibitory activities against Escherichia coli, with MIC value of 15.6 mg·L-1.关键词：Rodgersia aesculifolia;p-hydroxy benzoic acid;3β-hydroxyolean-12-en-27-oic acid;succinic acid
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical compositions of Mahuang tincture formula. Method: Compounds were isolated and purified from the formula with silica gel column chromatography, ODS reversed-phase column chromatography and recrystallization, and their structures were identified on the basis of spectral analyses and physicochemical properties. Result: Twelve compounds were isolated from the ethylacetate extract (with strongest anti-inflammatory activity) of Mahuang tincture and their structures were identified as berberine (1), columbamine (2), coptisine (3), 8-oxo-epiberberine (4), 8-oxo-coptisine (5), geniposide (6), brucine (7), strychnine (8), vomicine (9), α-amyrin (10), β-sitosterol (11), daucosterol (12). Conclusion: All 12 compounds were isolated from this formula for the first time and alkaloids were the main compositions, which must be the material basis for pharmacological activities of Mahuang tincture.关键词：Mahuang tincture;ethylacetate extract;alkaloids
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for simultaneous determining p-coumaric acid and cinnamic acid contents in Scutellaria barbata. Method: Eclipse SB-C18column (4.6 mm×250 mm,5 μm) was adopted with a mobile phase of acetonitrile-0.2% phosphoric acid at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 310 nm and 280 nm and the column temperature was 26℃. Result: The good linear range was 0.148-1.776 μg (r=0.9998) for p-coumaric acid and 0.062-0.992 μg (r=0.9996) for cinnamic acid. The average recovery rate was 99.4%(RSD 1.5%) and 99.3%(RSD 1.8%) for p-coumaric acid and cinnamic acid respectively. The sample solution was steady within 12 h. Through the determination of the 8 batches of S. barbata, results showed that the contents of these 2 components in Hubei samples (batch number:130501) were better than those of other areas. Conclusion: This method is precise and accurate for determination of p-coumaric acid and cinnamic acid contents in S. barbata.关键词：Scutellaria barbata;p-coumaric acid;cinnamic acid
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发布时间：2024-01-04 - 摘要：Objective: By establishing HPLC fingerprint of propolis, to evaluate the quality differences of propolis from different areas, and provide experimental evidence for appropriate choice of raw propolis origins. Method: The fingerprints of 14 batches of propolis from four main producing areas were determined by HPLC method, and the fingerprints were compared for similarity by using "Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" (2004 A) issued by Chinese Pharmacopoeia commission and cluster analysis. Result: The common mode for propolis fingerprints was established, and 12 common fingerprint peaks were identified;the similarity was greater than 0.90 in 9 batches of the 14 batches of propolis medicinal herbs, indicating that propolis fingerprints had both similarities and differences either from different main producing areas or different fields within the same producing area. Conclusion: This method is reproducible, precise and steady, and it can be used for quality control and origin identification of propolis medicinal herbs.关键词：propolis;HPLC;fingerprint;similarity assessment;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the content determination of gastrodin, 4-hydroxybenzyi aicohol, ferulic acid and ligustilide in extracts of Ligusticumc chuanxiong-Gastrodia elata. Method: The determination was performed on Inertsil ODS-3 chromatographic column (4.6 mm×250 mm, 5 μm) with mobile phase of methanol-0.05% phosphoric acid for gradient elute, at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 220 nm (gastrodin,4-hydroxybenzyi aicohol), 320 nm (ferulic acid,ligustilide) and column temperature was 25℃. Result: The four compounds were well separated and showed good linearity within the concentration ranges tested. Their average recovery rate was 104.0%, 103.4%, 100.6% and 101.8% for gastrodin, 4-hydroxybenzyi aicohol, ferulic acid and ligustilide;and RSD of the above four components was 0.4%, 0.5%, 4.5% and 2.6%, respectively. Conclusion: This method is simple, precise and repeatable, and it can be used to determine the four active components in Ligusticumc chuanxiong-Gastrodia elata.关键词：gastrodin;4-hydroxybenzyi aicohol;ferulic acid;ligustilide;Ligusticumc chuanxiong-Gastrodia elata
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发布时间：2024-01-04 - 摘要：Objective: To establish the fingerprints of the dry extracts of Ficus Herba and Keteling capsules by HPLC and to identify their main compositions by LC-Q-TOF-MS. Method: HPLC was used to establish the fingerprints of dry extracts of Ficus Herba, and liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) method was used to identify the main chromatographic peaks of the fingerprints. The analysis was performed on Acquity UPLC HSS T3C18column (2.1 mm×100 mm,1.7 μm), with gradient elution of methanol(A)-0.05% formic acid(B) at the flow rate of 0.2 mL·min-1 and the column temperature was 25℃. Part of the marked chromatographic peaks were identified. Result: HPLC fingerprints of the dry extracts of Ficus Herba were established, and the chemical constituents were identified by LC-Q-TOF-MS. 14 compounds were identified in this method. Conclusion: The established fingerprint method is suitable to the extracts of Ficus Herba and Keteling capsules, with accurate identification of the chemical compositions;it has improved the HPLC fingerprints, and provides a basis for the overall quality control of dry extracts of Ficus Herba and its prescriptions.关键词：HPLC;LC-Q-TOF-MS;dry extracts of Ficus Herba;Keteling capsules
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining the contents of ecliptasaponin C, ecliptasaponin IV, ecliptasaponin A, and echinocystic acid 28-O-β-D-glucoside in Eclipta Herba. Method: Samples were extracted with 50% MeOH, and the chromatographic separation was performed on Waters ACQUITY UPLC BEH C18column (2.1 mm×150 mm, 1.7 μm), with a mobile phase of acetonitrile-water at flow rate of 0.3 mL·min-1. PDA detection was carried out at a wavelength of 210 nm. The column was maintained at 30℃, and the injection volume was 5 μL. Result: The measured data showed that the recovery rate of the four compounds ranged from 95% to 105%, and their RSD values were all less than 3%. Conclusion: This method is simple, precise, and rapid, and could be used for the content determination of ecliptasaponin C, ecliptasaponin IV, ecliptasaponin A, and echinocystic acid 28-O-β-D-glucoside in Eclipta Herba.关键词：UPLC;Eclipta Herba;ecliptasaponin C;ecliptasaponin IV;ecliptasaponin A;echinocystic acid 28-O-β-D-glucoside
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发布时间：2024-01-04 - 摘要：Objective: To establish a simple and reliable quality control method for Qizhu Yiqi Runchang granules. Method: The contents of calycosinglucoside, echinacoside, andacteoside were determined by HPLC, and Astragali Radix, Atractylodes Macrocephalae Rhizoma, and Aurantii Fructus were identified by thin-layer chromatography(TLC). Result: The good linear ranges were 0.041 4-0.207 μg for calycosinglucoside, 0.146 7-0.733 5μg for echinacoside, and 0.035 1-0.175 5 μg for acteoside;correlation coefficient was 0.999 5, 0.999 5 and 0.999 6, respectively. The average recovery rate was 97.8%-102.2%, with RSD of 1.8%-2.3%.Simultaneously, the characteristic spots of Astragali Radix, Atractylodes Macrocephalae Rhizoma, and Aurantii Fructus were detected by TLC, and the negative control test showed no interference. Conclusion: This method is reproducible, simple and fast, and can be used to effectively control the quality of Qizhu Yiqi Runchanggranule.关键词：Qizhu Yiqi Runchang granule;calycosinglucoside;echinacoside;acteoside
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for quality control of Qingre Yangwei mixture. Method: The ingredients of Origani Herba and Trollii altaici Flos were identified by thin layer chromatography (TLC). Methylbenzene-ethyl aceate ethyl ethanoate-formic acid (10:7.5:1) was used as developing solvent in the TLC identification of Origani Herba, with caffeic acid as the control product;ethyl aceate ethyl ethanoate-formic acid-water (8:1:0.8) was used as developing solvent in the TLC identification of T. altaici Flos, with rutin and crude T. altaici Flos as the control products.The content of total flavonoids was determined by ultraviolet spectroscopy, and the content of rutin was determined by high performance liquid chromatography (HPLC). Result: The TLC method of Origani Herba and T. altaici Flos from Qingre Yangwei mixture had identification characteristics with clear spots, and showed the same identifying reaction with the control products. In the determination of total flavonoids content, the good linear range was 0.134-0.670 mg for rutin (r=0.999 9), with an average recovery rate of 98.42% and RSD of 1.2%. In the contents determination, the good linear range was 0.400-4.000 μg for rutin (r=0.999 9), with an average recovery rate of 98.91% and RSD of 1.2%. Conclusion: This identification method is selective and sensitive. The quantitative method is simple, rapid, accurate and reproducible, and can be used to control the quality of Qingre Yangwei mixture.关键词：Qingre Yangwei mixture;Origani Herba;Trollii altaici Flos;total flavonoids;rutin
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发布时间：2024-01-04 - 摘要：Objective: To develop a rapid,reliable and sensitive method of UPLC-MS/MS with an atmospheric-pressure chemical ionization(APCI) source for determination of tanshinone ⅡA and salvianolic acid B in rat plasma after oral administration of Yindan Xinnaotong soft capsules. Method: UPLC-MS/MS was employed to measure plasma concentration at different time after ig administration of Yindan Xinnaotong soft capsules with 2.4 g·kg-1.Analysis was detected by APCI in multiple reaction monitoring (MRM) mode.Detection of tanshinone ⅡA and salvianolic acid B was using following transitions of m/z 295.0-249.0 for tanshinone ⅡA,m/z 717.1-519.0 for salvianolic acid B and m/z 271.1-122.8 for baicalein.DAS 2.1.1 software was used to calculate pharmacokinetic parameters. Result: Calibration curve ranges were 1-100 and 5-500 μg·L-1 for tanshinone ⅡA and salvianolic acid B,respectively.Mean recovery of them ranged from 72.43% to 93.92%,intra-day and inter-day precisions were in the range of 2.73%-9.87% and accuracies were between 92.65% to 106.26%.Cmax of tanshinone ⅡA and salvianolic acid B were (38.34±17.35),(32.00±15.43) μg·L-1,Tmax of them were (0.25±0.23),(0.75±0.18) h. Conclusion: This method developed in the present study using UPLC-MS/MS is highly sensitive,accurate,rapid and suitable for pharmacokinetic study of tanshinone ⅡA and salvianolic acid B from Yindan Xinnaotong soft capsules in rats.关键词：UPLC-MS/MS;Tanshinone ⅡA;salvianolic acid B;pharmacokinetic;Yindan Xinnaotong soft capsules
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发布时间：2024-01-04 - 摘要：Objective: To establish an UPLC-MS method for determining the content of daidzein in rat plasma,and compare pharmacokinetic parameters of daidzein in rats after gavage of compound Naomaitong and Puerariae Lobatae Radix extract for evaluating effects of other ingredients in compound Naomaitong extract on pharmacokinetics of daidzein. Method: Twelve rats were randomly divided into two groups,gastric gavage of compound Naomaitong and Puerariae Lobatae Radix extract according to 1.002 5 mg·kg-1 of daidzein,plasma samples collected at different time,and then using UPLC-MS to determine plasma concentrations of daidzein,pharmacokinetic parameters were calculated by DAS 2.0 software,and its one-way ANOVA with SPSS 16.0 software. Result: There was a good linearity over concentration ranges of 6.175-741.0 μg·L-1,limit of quantitation was 6.175 μg·L-1,the lowest detection limit was 1.235 μg·L-1.Cmax and CL/F of daidzein from compound Naomaitong and Puerariae Lobatae Radix extract were significantly different,the other parameters were not significantly different. Conclusion: This UPLC-MS is sensitive,accurate,specific and applicable to determine in vivo plasma concentration of drug with daidzein as a main ingredient.Other ingredients in compound Naomaitong extract have a great impact on absorption and removal of daidzein,so that Cmax and CL/F of daidzein decrease.关键词：compound Naomaitong;Puerariae Lobatae Radix;daidzein;hesperidin;drug metabolism
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发布时间：2024-01-04 - 摘要：Objective: To reveal absorption,distribution and metabolic rule of serum fingerprint component group of traditional Chinese medicine by investigating fingerprint pharmacokinetic of Penthorum chinense. Method: Rats were administered orally,blood sampling by ophthalmic venous plexus,serum was separated by centrifugation.HPLC was employed to analyze serum samples with mobile phase of acetonitrile-0.1% formic acid for gradient elution,flow rate of 0.8 mL·min-1 and detection wavelength at 280 nm.Based on establishment of serum fingerprints of P. chinense,total constituents absorbed into blood were determind by comparing fingerprints of ethanol extract,medicated serum sample and blank serum sample. Result: Six components were found into the blood including three prototype components and three metabolic components.Concentration-time curves revealed that total concentration had reached saturation after 3 days and removed all components after 36 h. Conclusion: Six components may be basis for efficacy substances in P. chinense,change law of total concentration-time curves is similar with absorption,distribution and metabolism process in pharmacokinetics.Components group of serum fingerprint reaches saturation with long time,which is the same as traditional Chinese medicine (TCM) takes a slower effect and keep a long time.It can provide new ideas for fingerprint pharmacokinetics,pharmacodynamics material base and half-life study of TCM.关键词：Penthorum chinense;serum fingerprint;fingerprint pharmacokinetics;concentration-time curve;intragastrical adiministration
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of aqueous extract and monomer components of Biqi capsule on lipopolysaccharides (LPS)-induced inflammatory response of monocyte/macrophage cell line RAW 264.7 in mice, determine the major anti-inflammatory active components of Biqi capsule, and provide theory basis for the upgrade of quality control standards. Method: Acute inflammatory model was built by LPS-induced RAW 264.7 cells, and treated by aqueous extract of Biqi capsule (mass concentration of 0.25, 0.5, 1 mg·L-1) and major monomer components (brucine, strychnine, cryptotanshinone, salvianolic acid B, liquiritin and glycyrrhizic acid, mass concentration of 1×10-7, 1×10-6, 1×10-5 mol·L-1). After treatment for 24 h, nitric oxide (NO) and interleukin (IL) -6 were detected by Griess reagent assay and enzyme-linked immunosorbent assay (ELISA), respectively. Result: Compared with the normal group, the expressions of IL-6 and NO were significantly increased in LPS-induced RAW 264.7 cells of model group. Compared with the model group, both aqueous extract of Biqi capsule and cryptotanshinone could inhibit the increase of NO and IL-6;brucine, strychnine, glycyrrhizin and glycyrrhizic acid could inhibit NO secretion of RAW 264.7 cells. Conclusion: Cryptotanshinone, brucine and strychnine are the major anti-inflammatory components in aqueous extract of Biqi capsule.关键词：Biqi capsule;aqueous extract;Cryptotanshinone;nitric oxide;interleukin-6
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发布时间：2024-01-04 -
Effects of Sijunzi Tang on Glucose Absorption and Jejunum Mucosa Repairing of Spleen Deficiency Rats
摘要：Objective: To observe the effects of Sijunzi Tang on glucose absorption and the jejunum mucosa repair in spleen deficiency rats,and investigate the mechanism of Sijunzi Tang for replenishing Qi and invigorating the spleen. Method: The SD rats were divided randomly into 6 groups:normal group, model group, spermidine group(7.5 mg·kg-1) and Sijunzi Tang high dose group, middle dose group and low dose group (22.0,11.0,5.5 g·kg-1). Spleen deficiency models were prepared by reserpine via subcutaneous administration in all rats except normal group. In situ single-pass intestinal perfusion models were used to determine rat intestinal absorption of glucose in different groups by ultraviolet spectroscopy. Light microscope was used to observe the pathological changes of jejunum tissues, and compare the repairing conditions of mucosal injury. Result: The glucose absorption rate constant (Ka) and effective permeability coefficient (Papp) in different groups showed that the glucose absorption was significantly lower in the model group than that in the normal group and the injury of jejunum mucosa was more obvious (P<0.05). Compared with the model group, the glucose absorption was significantly increased in rats of spermidine group and Sijunzi Tang groups, and the injury of jejunum mucosa was significantly repaired (P<0.05). Conclusion: Sijunzi Tang could improve the absorption of glucose via repairing the damaged structure of intestinal mucosa of spleen deficiency rats, which may be one of the possibly important mechanisms for replenishing Qi and invigorating the spleen.关键词：Sijunzi Tang;spleen deficiency;glucose;absorption;mucosa- 4
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of the containing serum of Danzhi Xiaoyao powder on human breast cancer cell line MCF-7 by regulation of autophagy. Method: Rats were intragastrically given with 8.99 g·kg-1 Danzhi Xiaoyao powder to prepare the containing serum. The exponential phase MCF-7 cells were treated with containing serum of Danzhi Xiaoyao powder was inoculated on 96-well plates with the density of 1×105 cells/mL, 100 μL per well. The cells were divided into blank serum group and containing serum group, with 3 double-wells in each group. They were treated for 12, 24, 48, 72 h respectively, and then methyl thiazolyl tetrazolium (MTT) assay was used to measure its inhibitory effects on the growth of MCF-7 cells;the formation of autophagic vacuoles was detected using monodansylcadaverine (MDC) fluorescence microscope, and the expression of Bclin1 protein was detected using Western blotting. Result: Compared with the blank serum group, the growth of MCF-7 cells was significantly inhibited by 15% Danzhi Xiaoyao powder's containing serum, and the optimal condition was achieved after treatment for 48 h. At this time point, MDC staining fluorescence intensity and the formation of autophagic vacuoles were increased;at the same time, the expression of Beclin1 in MCF-7 cells was significantly up-regulated. Conclusion: Containing serum of Danzhi Xiaoyao powder could significantly inhibit MCF-7 breast cancer cells proliferation and induce autophagic death of cells. This may be one of the important mechanisms of its anti-breast cancer.关键词：Danzhi Xiaoyao powder;autophagy;breast cancer;MCF-7 cells
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Se-enriched Ganoderma lucidum polysaccharides on blood lipids, inflammatory factors, stearoyl coenzyme1 (SCD1) and peroxisome proliferator-activated receptor α (PPARα) expression in rats with nonalcoholic fatty liver disease (NAFLD). Method: One hundred and twenty SD rats were randomly divided into normal group, high-fat diet group (model group), polyene phosphatidylcholine(50 mg·kg-1) group, Se-enriched G. lucidum polysaccharides low, medium and high dose groups (0.3, 0.6, 1.2 g·kg-1,ig). Rats were given high-fat and high-sucrose diet for 12 weeks to establish NAFLD models. After treatment for 4 weeks and 8 weeks, the rats were sacrificed half and half to detect high-density lipoprotein-C (HDL-C), low-density lipoprotein-C (LDL-C), interleukin-1α (IL-1α), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) in serum, as well as SCD1 and PPARα levels in liver homogenates. Result: Compared with the normal group, fat vacuoles were obvious and associated with inflammation after 4 weeks treatment in model group. Compared with the model group, Se-enriched G. lucidum polysaccharides treatment groups significantly decreased fat particles. Compared with the normal group, HDL-C, LDL-C, IL-1α, IL-1β and TNF-α levels were significantly increased in the model group after 8 weeks of treatment, Compared with the model group, HDL-C, LDL-C, IL-1α, IL-1β and TNF-α levels were significantly decreased in three Se-enriched G. lucidum polysaccharides treatment groups (P<0.05), and they were further decreased after 8 weeks of treatment, but with no significant difference from the polyene phosphatidylcholine group. Compared with the normal group, SCD1 and PPARα mRNA and protein expressions in liver tissues were significantly decreased in model group after 4 weeks treatment. Compared with the model group, SCD1 and PPARα mRNA and protein expressions in liver tissues were significantly increased in three Se-enriched G. lucidum polysaccharides treatment groups, but the effect of polyene phosphatidylcholine was better than Se-enriched G. lucidum polysaccharides on the improvement of SCD1 and PPARα mRNA and protein expressions. Conclusion: Se-enriched G. lucidum polysaccharides could improve SCD1 and PPARα expressions in liver tissues of NAFLD rats, and could regulate blood lipids, and inflammation although its effect was inferior to polyene phosphatidylcholine.关键词：Se-enriched Ganoderma lucidum polysaccharides;nonalcoholic fatty liver disease;stearoyl-coa desaturase1;peroxisome proliferator-activated receptor α
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Sishen Wan on the balance of Treg/Th17 in Payer's patch of colitis rats. Method: The forty SD rats were randomly divided into 4 groups:normal group, model group, Sishen Wan group (2.5 g·kg-1) and mesalazine group (0.15 g·kg-1). Except the normal group, all other groups established the rat colitis models induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). Drugs were given intragastrically for 7 days. The levels of T lymphocyte subsets were measured by flow cytometry, and the ratio of Treg/Th17 was then calculated. The expressions of retinoid acid-related orphan receptor γt (ROR-γt) and signal transducer and activator of transcription 5a (STAT5a) were tested by the Western blot. Result: Compared with the normal group, the model group significantly increased the levels of CD4+T lymphocyte, significantly decreased CD8+T cells, significantly increased the ratio of CD4/CD8, significantly decreased CD4+CD25+Foxp3+Treg cells, significantly increased Th17 cells, decreased the ratio of Treg/Th17, increased the expressions of ROR-γt protein in colon tissues, and significantly decreased the expressions of STAT5a protein (P<0.05, P<0.01). Compared with the model group, Sishen Wan group significantly decreased CD4+T lymphocyte in Payer's patch of rats, significantly increased CD8+T cell levels, and significantly decreased the ratio of CD4/CD8 (P<0.01). At the same time, Sishen Wan group significantly increased CD4+CD25+Foxp3+Treg cells, decreased Th17 cells, increased the ratio of Treg/Th17, decreased the expressions of ROR-γt protein in colon tissues, and significantly increased the expressions of STAT5a protein (P<0.05,P<0.01). Conclusion: The Sishen Wan could regulate the T lymphocyte subsets, and by inhibiting the expressions of RORγt and increasing the protein secretion of STAT5a, it can also regulate the balance of Treg/Th17.关键词：colitis;Sishen Wan;T lymphocytes;Treg/Th17
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of medicated serum prepared with Jiangya Baolan tablets on the human umbilical veinendothelial cells (HUVECs) injured by hydrogen peroxide. Method: HUVECs were cultured in vitro and used for experiment. They were divided into five groups as follows:blank group, model group, Jiangya Baolan tablets low dosage group, mid-dosage group and high-dosage group (volume fraction of 5%, 10%, 15% respectively). The morphological change of the cells was observed under microscope. MTT was used to determine the HUVECs viability. The levels of lactate dehydrogenase (LDH), and nitric oxide (NO) in supernatant were measured by the microplate method;the levels of endothelin-1(ET-1), tissue plasminogen activator(tPA) and plasminogen activator inhibitor-1(PAI-1) were determined by enzyme-linked immunosorbent assay (ELISA). Result: Compared with the blank group, the model group significantly decreased HUVECs viability, significantly increased the levels of LDH in supernatant, significantly increased ET-1 and PAI-1, and significantly decreased the levels of NO and tPA (P<0.01). Compared with the model group, Jiangya Baolan tablets mid-dosage and high-dosage groups significantly increased HUVECs viability, significantly decreased the levels of LDH, ET-1, PAI-1, and significantly increased the levels of NO and tPA (P<0.05,P<0.01). Conclusion: Medicated serum prepared with Jiangya Baolan tablets has significant protective effect on HUVECs injured by H2O2.关键词：Jiangya Baolan tablets;medicated serum;human umbilical veinendothelial cells;hydrogen peroxide
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Danzhi Jiangtang capsules on impaired endothelial function in rats models with type 2 diabetes mellitus (T2DM) based on p38 mitogen-activated protein kinase (p38MAPK) signal pathways. Method: High fat and high glucose diet and intraperitoneal injection of streptozotocin (STZ) were used to establish T2DM rat models. One hundred and four rats were randomly divided into 7 groups:normal group, model group, Danzhi Jiangtang capsule high, medium, low dose groups (1.08, 0.72, 0.54 g·kg-1·d-1), pioglitazone group (10 mg·kg-1·d-1), integrated Danzhi and pioglitazone group (1.08 g·kg-1·d-1+10 mg·kg-1·d-1). After successful modeling, the diabetic model rats were respectively ig given with corresponding dose of medicines, once a day. After 8 weeks of treatment, blood samples and abdominal aorta were collected to do correlation detection after rats anesthesia, and their mechanism of action was analyzed. Result: After 8 weeks of treatment, compared with the normal group, p-p38 mitogen activated protein kinase(p-p38MAPK), mitogen activated protein kinase kinasse3/6(MKK3/6), cAMP response element-bingding protein(CREB1), cyclooxygenase2(COX-2), and intercellular cell adhesion molecule-1(ICAM-1) protein expression levels were increased in model group;mitogen-activated protein kinase phosphatase-1(MKP-1) protein expression level was reduced in the model group. Compared with the model group, p-p38MAPK, MEK3/6, CREB1, COX-2, and ICAM-1 protein expression levels were reduced, and MKP-1 protein expression levels were increased in treatment groups (P<0.05, P<0.01). The abdominal aorta immunohistochemistry showed that compared with the model group, the expression level of p38MAPK was significantly decreased in the treatment groups. Conclusion: Danzhi Jiangtang capsule can regulate the expression level of vascular p38MAPK protein in T2DM rat models and may thus improve the impaired endothelial function in rats.关键词：p38 mitogen-activated protein kinase;signal pathway;diabetic vascular disease;Danzhi Jiangtang Capsule;immunohistochemistry
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective mechanism of Shenfu Qiangxin pill(SFQX) on heart and kidney cells apoptosis in rats with cardio-renal syndrome (CRS) based on the (pro)rennin receptor (PRR)-mediated mitogen-activated protein kinase(MAPK) signal pathways. Method: Wistar rats underwent reperfusion injury combined with abdominal aortic constriction after renal ischaemia to prepare CRS models. The CRS rats were divided randomly into three groups:CRS model group (10 mL·kg-1pure water, ig);CRS+SFQX group (SFQX group, 13.2 g·kg-1 SFQX, ig);CRS+Handle region peptide (HRP)group (10 mg·kg-1 HRP, iv). The rats in sham operation group were ig given with the same volume of pure water. Treatments were given 8 weeks after surgery, 1 time/day, for 4 weeks. After the experiment, the rats were detected for brain natriuretic peptide (BNP), urea nitrogen (BUN), and creatinine (Cr) levels;ultrasound cardiograph for small animals was used to detect end diastolic interventricular septum thickness (IVS), left ventricular end diastolic posterior wall thickness (LVPW) and left ventricular ejection fraction (LVEF);real time fluorescent PCR was used to measure PRR mRNA expressions of left ventricle and kidney;Western blot assay was used to determine MAPK signal pathways, including extracellular signal-regulated kinase 1/2 (ERK1/2), C-Jun amino terminal kinase (JNK), and P38 protein expressions;Terminal deoxynucleotidyl transferase dUTP nick and labeling (TUNEL) was used to detect cells apoptosis of left ventricular and renal tissues. Result: Compared to the sham operation group, serum BNP, BUN and Cr levels were significantly increased (P<0.05);IVS, LVPW levels were significantly increased (P<0.01);LVEF levels were significantly decreased (P<0.01);mass index of left ventricle was significantly increased (P<0.01);mass index of left kidney was significantly decreased in CRS rats(P<0.01).Expression of PRR mRNA was increased (P<0.01);protein expressions of ERK1/2, p38 and JNK were increased (P<0.01). Myocardial and renal cell apoptosis rate was 32.5% and 63.2% respectively.SFQX 13.2 g·kg-1could significantly reduce cardiac hypertrophy in CRS rats, inhibit IVS and LVPW, increase EF, significantly decrease BUN and Cr, decrease PRR mRNA expression and ERK1/2, P38 protein expression of injured tissues, and decrease the myocardial and renal cell apoptosis rate. Conclusion: SFQX could improve the heart and kidney function of CRS rats, and reduce myocardial and renal cell apoptosis through reducing PRR mRNA expression of myocardial and renal cells and inhibiting ERK1/2, JNK and P38 phosphorylation in MAPK signal pathways.关键词：Shenfu Qiangxin pill;mitogen-activated protein kinase;C-Jun amino terminal kinase;infrarenal aortic-clamping;renal ischaemia;cardio-renal syndrome;(pro)rennin receptor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of Rhei Radix et Rhizoma on intestinal flora of septic rats. Method: LPS intraperitoneal injections were used to establish septic rats models. The rats in the experiment were divided into five groups:sham operation group, septic model group, Rhei Radix et Rhizoma high dose, middle dose and low dose groups (150, 100, 50 mg·kg-1), 2 times/d ig, continuous for 3 days. The mortality of each group was recorded. Traditional bacterial culture method was used to detect bacteria count of membrane flora, cavity flora, mesenteric lymph nodes and blood of the rats. Meanwhile aquaporin 2(AQP2) and AQP8 were detected by RT-qPCR and Western blot. Result: The mortality in Rhei Radix et Rhizoma high dose group and middle dose group was significantly decreased. Membrane flora and cavity flora of all groups differ greatly, especially Escherichia coli, Staphylococcus aureus, Lactobacill and Bifidobacterium. AQP2 and AQP8 expression levels of all treated groups differed greatly from those of model group(P<0.05). The decrease in AQP2 and AQP8 expression levels in various treatment groups was dependent on the dose of Rhei Radix et Rhizoma. meanwhile, the number of pathogens in lymph nodes and blood was lower than that of model group. Conclusion: Rhei Radix et Rhizoma can decrease bacterial translocation by reducing expression of AQPs to increase excreta in treating sepsis, and the middle dose is better for the balance of intestinal flora.关键词：Rhei Radix et Rhizoma;sepsis;intestinal flora;aquaporins
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of jolkinolide B on proliferation of MDA-MB-231 cells and cell cycles in human breast cancer. Method: MDA-MB-231 cells in logarithmic phase were selected and added with jolkinolide B, to achieve the final concentrations of 0, 2.5, 5, 10, 20, 40, 80 mg·L-1. MTT method was used to measure the growth inhibitory rate of jolkinolide B on MDA-MB-231 cells. For 10, 20, 40, 80 mg·L-1groups, the effect of jolkinolide B on cell cycle of MDA-MB-231 cells was assayed by flow cytometer. The mRNA and protein expression levels of eukaryocytic translation initiation factor 4E (eIF4E) or CyclinD1 were detected by RT-PCR and Western blot assay. Result: With the increase of concentrations of jolkinolide B, it could significantly inhibit the proliferation of MDA-MB-231 cells and block the cells at G1 phase. Compared with the blank group, 20, 40 μmol·L-1 jolkinolide B could inhibit mRNA expressions of CyclinD1 and eIF4E, and inhibit the mRNA and protein expressions of CyclinD1 in transfected eIF4E-siRNA group, and meanwhile, the cell cycle was blocked in G1 phase. Conclusion: The jolkinolide B could block the cell cycle in G1 phase by down-regulating the expression of eIF4E and CyclinD1.关键词：jolkinolide B;eukaryocytic translotion initiation factor 4E;cell cycle
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发布时间：2024-01-04 - 摘要：Objective: To study the antibacterial activities of different extract parts from Dryopteridis Crassirhizomatis Rhizoma. Method: The crude extracting of Dryopteridis Crassirhizomatis Rhizoma was extracted in 70% ethanol for 72 h, and the extracts of five fractions (petroleum, chloroform, EtoAc, n-BuoH and water) were prepared with liquid-liquid separation method. The antibacterial activities of different fractions on six strains (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus flavus and Rhizopus nigricans) were studied by K-B paper dispersion. Result: All five parts had certain antibacterial effects on test strains, and the overall antibacterial activities were increase with the increase of the drug dose. The chloroform and EtoAc fractions had the stronger antibacterial activities, and the water fraction had the minimum antibacterial activities. Conclusion: Different fractions had different antibacterial activities on test strains.关键词：Dryopteridis Crassirhizomatis Rhizoma;extract fractions;bacteria;Fungi;antibacterial activities
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of icariin (ICA) on the proliferation of esophageal cancer cells EC9706 and identify its mechanism of action. Method: EC9706 cells were stimulated by different concentrations of ICA for 48 h, then CCK8 assay was performed to detect the proliferation of EC9706 cells;Hoechst33342 staining and fluorescence microscope were used to detect cells apoptosis;reverse transcription-polymerase charin reaction(RT-PCR) was used to detect B-cell lymphoma/leukemia2(Bcl-2) and Bcl-2 associated xprotein(Bax) mRNA expression,Western blot was used to detect Bax and Bcl-2 protein expression. Result: ICA could significantly inhibit proliferation of EC9706 cells. Cell apoptosis morphological changes were found in EC9706 cells after ICA treatment.Western blot results showed Bax protein expression was decreased, while Bcl-2 protein expression was increased. Conclusion: ICA could significantly inhibit the proliferation of human esophageal cancer EC9706 cells, and the mechanism of apoptosis may be associated with up-regulating the expression of Bax protein and down-regulating the expression of Bcl-2 protein.关键词：icariin;esophageal cancel;EC9706 cells
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Liuwei Dihuang Wan on learning and memory abilities and activity of cholinergic system in APP/PS1 mice. Method: 40 double transgenic APP/PS1 mice were randomly divided into APP/PS1 model group, Liuwei Dihuang Wan low dose group (0.59 g·kg-1·d-1), medium dose group (1.18 g·kg-1·d-1), and high dose group (2.36 g·kg-1·d-1), ibuprofen group (0.04 g·kg-1·d-1), with 8 mice in each group. Another 8 3 month old wild type mice (WT) were selected as the normal group. After lavage administration for 3 months, Morris water maze was used to test their learning and memory abilities. The content of acetylcholine (ACh) and the activity of choline acetyltransferase (ChAT) and acetylcholinesterase(AChE) in cortex were measured by enzyme-linked immunosorbant assay(ELISA). Result: Compared with the normal control group, the time required to find the platform (latency) was longer in the model group of each period (P<0.05);time and percentage of staying in platform quadrant and frequency of crossing the platform were decreased (P<0.05);content of ACh and activity of ChAT were decreased (P<0.05). Compared with the APP/PS1 model group, latency was shortened (P<0.05), time and percentage of staying in platform quadrant and frequency of crossing the platform were increased (P<0.05) in Liuwei Dihuang Wan low, medium and high dose groups as well as ibuprofen group;the content of ACh and the activity of ChAT were increased (P<0.05), while the activity of AChE was not significantly different. Conclusion: Different doses of Liuwei Dihuang Wan can improve learning and memory abilities of APP/PS1 mice, which is related to its effects on the cholinergic system by increasing the content of ACh and the activity of ChAT.关键词：Liuwei Dihuang Wan;APP/PS1 mice;Morris water maze;acetylcholine
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发布时间：2024-01-04 -
Effect and Mechanism of Yangxue Huayu Xiaozheng Decoction on Formation of Endometriosis Foci in Rats
摘要：Objective: To study the effect and mechanism of Yangxue Huayu Xiaozheng decoction on formation of endometriosis foci in rats. Method: Female SD rats were selected and all the other rats except 10 rats in normal group were used to establish endometriosis models with auto grafting method. The successfully modeled rats were randomly divided into six groups, including model group, Yangxue Huayu Xiaozheng decoction high-dose group, middle-dose group, low-dose group (12, 8, 4 g·kg-1,respectively) and Gestrinone group (0.05 g·kg-1), n=10 in each group. After ig administration respectively for 28 days, the lesion tissue samples of rats were collected, and the inhibitory effect of Yangxue Huayu Xiaozheng decoction on endometriosis tissue in rats was observed. The expression of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor1 (VEGFR-1) were detected in ectopic endometrium by using RT-PCR and Western blot technique. Result: Compared with the normal group, the expression of VEGF and VEGFR-1 mRNA and protein in endometriosis tissues of rats in model group was significantly higher (P<0.05). Compared with the model group, endometriosis tissues of rats in drug groups were significantly inhibited, and the expression of VEGF and VEGFR-1 mRNA and protein in endometriosis tissues of rats in various drug groups was significantly lower than that in model group (P<0.05). Conclusion: Yangxue Huayu Xiaozheng decoction could obviously inhibit the development of endometriosis tissues in model rats. It may have a closely relation with the inhibition of VEGF/VEGFR signaling pathway to regulate the angiogenesis of lesions.关键词：Yangxue Huayu Xiaozheng decoction;vascular endothelial growth factor;vascular endothelial growth factor receptor 1- 2
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发布时间：2024-01-04 - 摘要：Objective: To study the accompanied hepatotoxicity effect of Leigongteng tablet under anti-inflammation dose, and provide theory basis for 'disease-efficacy-toxicity' correlation research. Method: The 80 male KM mice were randomly divided into 8 groups, in each group, including normal group, model group, 5 Leigongteng tablet groups (51.48, 36.55, 25.74, 18.28, 12.87 μg·kg-1) and cyclophosphamide group (73 mg·kg-1). All other groups except normal group received 1% 2,4-dinitrofluorob-enzene(DNFB) solution to establish delayed type hypersensitivity models. Leigongteng tablets of different doses were given to observe the ear swelling degree and the inhibition ratio. The levels of prostaglandin E2(PGE2), tumour necrosis factor α (TNF-α), interleukin-2(IL-2), alanine aminotransferase (ALT), aspartate aminotransferase (AST), prealbumin(PA), total bile acid(TBA), total bilirubin (TBIL) in serum were detected while the ratios of organs to body weight were measured. Result: Compared with the normal group, the mice in model group had ear swelling, and the levels of PGE2, TNF-α, IL-2 and PA in serum were significantly increased (P<0.01). Compared with the model group, Leigongteng tablet could significant inhibit ear swelling and the levels of PGE2, TNF-α, and IL-2 were decreased in a dose dependent manner;the activities of serum ALT, AST, TBA and TBIL were increased;PA content was decreased;and the ratio of liver to body was increased. Conclusion: Leigongteng tablet has strong anti-inflammatory effects under the dose of 14.58 μg·kg-1 (7.01-19.06 μg·kg-1). The mechanism is related to the generation and decrease of inflammatory medium release. Toxicity and side effects of different degree can be caused to liver by administration of Leigongteng tablet at the same dosage.关键词：Leigongteng tablet;delayed-type hypersensitivity;efficacy;toxicity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of polysaccharide extract obtained from the Cestrum nocturnum(PCN)on the apoptosis of human osteosarcoma cell line MG-63 as well as it possible mechanism. Method: PCN was separated, and MG-63 cells in logarithmic phase were processed using PCN with the final concentration of 2.5, 5, 10 mg·L-1. Blank group was established with MG-63 cells. After 72 h of PCN (different mass concentration) action, MTT assay was used to determine the inhibition of cell proliferation. After 46 h of PCN (different mass concentration) action, transmission electron microscope was used to observe the morphological changes of processed cells. Its effect on apoptosis of MG-63 cells was detected by flow cytometry, and the protein expression of Caspase-3, Caspase-8, Caspase-9 and Bcl-2 were detected by Western blot. Result: PCN significantly inhibited the proliferation of MG-63 cells in a time and dose-dependent manner. It could significantly inhibit cells proliferation within the concentration range of 5-10 mg·L-1. Under electron microscopy, it could be found that PCN can induce cell apoptosis, with a large number of vacuoles in cytoplasm, and chromatin pyknosis was present for cell nucleus, and villus on cell nucleus surface was reduced. Flow cytometry indicated that 5 mg·L-1 can make MG-63 cells blocked in G0/G1. PCN induced apoptosis of MG-63 cells in a dose dependent manner, reduced Bcl-2 expression and increased Caspase-8 expression in a concentration dependent manner (P<0.05, P<0.01). Conclusion: PCN could effectively inhibit cell proliferation and induce MG-63 cells apoptosis, with a direct relation to the dose and time. This process may be associated with down-regulating protein expressions of Bcl-2 and activating the expressions of Caspases-8.关键词：polysaccharide extracted from Cestrum nocturnum;osteosarcoma;MG-62 cells;apoptotic
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Jishen capsule in models of ADR nephropathy rats by regulating cytokines, and explore the mechanism of action. Method: The models of ADR rats were established by twice intravenous injection of adriamycin and randomized into model group, positive ambrette capsule group (1.88 g·kg-1), Jishen capsule high, medium and low dose groups (2.25, 1.13, 0.56 g·kg-1), and a normal group. 10 rats was included in each group. Seven days later, drug administration was started. 24 hour urine protein quantity was examined in each group on day 7, 14, and 28. Blood biochemical indicators of total protein (TP), triglyceride (TG), total cholesterol (TC), albumin (Alb), serum creatinine (SCr), and urea nitrogen (BUN) were detected. Serum tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), nuclear factor-κB (NF-κB), transforming growth factor-β1(TGF-β1) were measured by ELISA. Activity of superoxide dismutase (SOD), malonaldehyde (MDA) were detected in the serum and renal pathological changes were observed by HE staining. Result: Compared with the normal group, 24 h proteinuria, TG, TC, SCr, BUN contents in serum and levels of TNF-α, IL-8, NF-κB, TGF-β1,MDA were significantly increased, while TP, Alb contents and serum SOD activity were significantly reduced in model group (P<0.01). Jishen capsule high-dose group could significantly reduce 24 h proteinuria of the ADR nephropathy rats, decrease the contents of TG, TC, SCr, BUN (P<0.05, P<0.01) and significantly increase the level of TP, Alb in serum (P<0.05, P<0.01), significantly reduce the cytokines level of TNF-α, IL-8, NF-κB, TGF-β1 in serum (P<0.05, P<0.01), increase the activity of serum SOD and decrease serum MDA level (P<0.05), relieve the pathological changes of renal tissues and improve renal function. Conclusion: Jishen capsules can treat the rats with ADR nephropathy by regulating cytokines levels of TNF-α, IL-8, NF-κB, and TGF-β1 in serum, with protective effects on renal tissues.关键词：Jishen capsule;cytokines;ADR nephropathy;mechanisms
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Kangmin Zhike mixture by oral taking or aerosol inhalation in treating children with cough variant asthma (CVA) and its influence to quality of life. Method: Eighty seven patients were randomly divided into control group (42 cases) and observation group (45 cases) by random number table. Patients in control group received montelukast sodium chewable tablets before sleeping at night, 4 mg for children who were less than 6 years old and 5 mg for children who were more than 6 years old, 1 time/day. Based on the treatment of control group, patients in observation group received additional Kangmin Zhike mixture by oral taking or aerosol inhalation. The treatment course was 4 weeks in both groups. Before and after treatment, scores of cough, ecphysesis, throat itching, expectoration and other symptoms were graded.Leicester cough questionnaire (LCQ) was made. And levels of the total IgE and peripheral blood eosinophils (EOS) in serum were detected. Result: Ridit analysis showed that, the clinical effect in observation group was superior to that in control group (P<0.05). Scores of nighttime cough, daytime cough and ecphysesis in observation groups were less than those in control group (P<0.01). Three dimension scores and total scores for LCQ in observation group were less than those in control group (P<0.01). Levels of the total IgE and EOS in serum were lower than those in control group (P<0.05). Conclusion: Kangmin Zhike mixture by oral taking and aerosol inhalation in the treatment of CVA can relieve inflammatory response in airway, ameliorate cough, and improve quality of life for patients, with significant clinical effect.关键词：cough variant asthma;children;Kangmin Zhike mixture;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To discuss the curative effect of Jijiao Lihuang Wan as an additional treatment for lung cancer accompanied by pleural effusion (PE) and effect on immunologic function. Method: One hundred and ten patients of lung cancer accompanied by PE were randomly divided into control group and treatment group in accordance with different treatment methods 55 cases.The drainage by central venous catheters was applied to all patients. Patients of control group were treated with nedaplatin 80 mg·m-2, Agglutinative Staphylococcin injection 4 mL and dexamethasone 10 mg, all of which were diluted by 0.9% sodium chloride and then injected intravenously, 1 time/2 weeks. Based on the treatment of control group, the patients in treatment group were also treated with Jijiao Lihuang Wan (1dose/d, decocted twice by oral taking) as the basic prescription. The treatment course was 6 weeks for both groups. The main clinical symptoms, hydrothorax and Karnofsky performance score (KPS) were observed for both groups before and after treatment. The changes in subgroups of T lymph cells were detected in PE of two groups. Result: Scores of main clinical symptoms were significantly lower in treatment group than those in control group after treatment (P<0.01). The total effective rate was 87.27% in treatment group, higher than 69.09% in control group, with statistical difference (P<0.05). The effective rate of KPS scores was 92.73% in treatment group,higher than 72.73% in control group, with statistical difference (P<0.05). After treatment,levels of CD3+, CD4+ and CD4+/CD8+ in treatment group were higher than those in control group, while level of CD8+ was lower in treatment group, with statistical difference (P<0.01). Conclusion: Based on clinical treatment of Western medicine, Jijiao Lihuang Wan as an adjunctive therapy for lung cancer accompanied by PE could improve clinical symptoms obviously, control the occurrence of PE, improve quality of life and increase immunologic function of the patients.关键词：Jijiao Lihuang Wan;lung cancer;pleural effusion;immunologic function
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发布时间：2024-01-04 - 摘要：Objective: To explore the curative effect and clinical feasibility of cervical vertebra restoration pill combined with mechanical balance pillow in treating vertebral artery type cervical spondylosis. Method: From Mar 2011 to Apr 2013,160 patients with vertebral artery type cervical spondylosis were treated with cervical vertebra restoration pill combined with mechanical balance pillow. Another 160 patients as control group were treated by taking gastrodia tuder halimasch tablets with cervical traction.They were treated with 10 days per course,and the next course began after 1 day of rest, in a total of 3 courses. After one year of treatment, they were evaluated for the curative effect according to changes of transcranial Doppler ultrasonography and cervical arch roof pitch changes, cervical vertigo symptom and function scores. Result: Test results showed thatthe improvement of blood flow during the systolic peak period of the vertebral basilar artery and the end diastasis period, cervical arch roof pitch, cervical vertigo symptom and improvement of function scores after treatmentin the treatment group were significantly greater than those in control group, withstatistically significant difference between these two groups (P<0.05). In respect of clinical efficacy, the effective rate was 96.2% in treatment group, higher than 72.5% in control group, with statistically significant difference between these two groups (P<0.05). Conclusion: The method of cervical vertebra restoration pillcombined with mechanical balance pillow has significant efficacy in treating vertebral artery type cervical spondylosis, can improve the physiological curvature of cervical spine, improve the compliance of vertebral artery and thus increase the brain blood supply, and reconstruct biomechanical stability of cervical vertebra.关键词：cervical vertebra restoration pill;mechanical balance pillow;vertebral artery typecervical spondylosis;traction
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发布时间：2024-01-04 - 摘要：Objective: To investigate the curative effect of Gishao capsule combined with irbesartan for the patients with diabetic nephropathy (DKD). Method: The 84 patients with DKD from January 2014 to April 2015 were enrolled into the study and randomly divided into observation group and control group, n=42 in both groups. Patients in the control group received irbesartan 150 mg qd. Based on it, patients in the observation group also received Gishao capsule, 5 pills tid. The sugar metabolism index and renal index were compared between two groups before and after treatment. And the curative effect were also compared between two groups. Result: The fasting plasma glucose (FPG), glycosylated hemoglobin (HbA1c) in the observation group were all significantly lower than the control group after treatment (P<0.05). Serum creatinine (SCr), urinary albumin excretion rate (UAER), 24 h urine protein (24 hUP), microalbuminuria (mALB), ratio of microalbuminuria to creatinine (mALB/UCr) and blood urea nitrogen (BUN) in the observation group were (135.7±18.9) μmol·L-1, (155.3±28.1) mg·(24 h)-1, (1.53±0.44) g, (39.7±12.9) μg·L-1, (15.5±3.9) mg·g-1, (6.59±0.87) mmol·L-1 respectively, which were all significantly lower than (163.6±18.0) μmol·L-1, (170.6±32.3) mg·(24 h)-1, (1.79±0.52) g, (47.4±14.4) μg·L-1, (17.7±3.8) mg·g-1, (7.06±0.93) mmol·L-1 in the control group (P<0.05). The curative effect in the observation group was significantly higher than that in the control group (P<0.05). Conclusion: Compared with irbesartan alone, Qishao capsule combined with irbesartan could improve the renal function of DKD patients more significantly, delay the progress of disease and have significant curative effect, which fully shows the unique advantage of Chinese with western medicine therapy and is worthy of clinical popularization and application.关键词：Qishao capsule;diabetic nephropathy;irbesartan
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of Xuebijing injection on elderly patients with chronic obstructive pulmonary disease(COPD) in acute exacerbation. Method: The 82 cases of elderly patients with COPD in acute exacerbation were randomly divided into two groups. Based on symptomatic and supportive treatments, the patients in control group were treated with 1.5 g cefoperazone sodium and sulbactam sodium which was diluted by 100 mL 5% glucose injection for intravenous drip, bid. Based on the treatment of control group, the patients in observation group also received 50 mL Xuebijing injection plus 100 mL normal saline, injection, by intravenous drip, bid. The treatment course was 14 days for both groups. The disease severity score, pulmonary function and the level of inflammatory factor before and after treatment were compared between two groups. Result: Compared with the control group, the total disease severity scores, level of forced expiratory volume in first second (FEV1), FEV1/forced vital capacity (FVC), C-reactive protein (CRP) and calcitonin (PCT) of the observation group were obviously improved after the treatment (P<0.05). The effective rate of the treatment group was 90.24%, higher than 80.49% in control group (P<0.05). Conclusion: For elderly patients with chronic obstructive pulmonary disease in acute exacerbation, Xuebijing injection can significantly improve clinical symptoms, signs and the level of pulmonary function, reduce inflammatory reaction, and improve the clinical efficacy.关键词：acute exacerbation of chronic obstructive pulmonary disease;Xuebijing injection;elderly patients;clinical effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Danhong injection on blood rheology, inflammatory cytokines and endothelial function in patients with thrombotic shallow phlebitis. Method: Sixty-three patients with thrombotic shallow phlebitis hospitalized from February 2014 to January 2015 in our hospital were randomly divided into two groups. The patients in control group received aspirin enteric-coated metformin hydrochloride and low molecular weight heparin calcium injection treatment, on the basis of control group, the patients in research group also received Danhong injection for 2 weeks. Then the curative effect, hemodynamic changes, inflammation, and endothelial function before and after treatment of two groups were observed and compared. Result: ①Curative effect:total effective rate in research group was 92.86%, higher than 76.19% in control group, with statistically significant difference between group (P<0.05). ② Blood rheology:after treatment, blood plasma viscosity, whole blood high shear reductive viscosity, whole blood low shear reduction viscosity, blood sedimentation, erythrocyte aggregation index, erythrocyte deformation index, and fibrin had varying degrees of decline in two groups, with significant difference (P<0.05);and compared with the control group, the decline was more significant in research group, with significant difference (P<0.05). ③Inflammatory cytokines:after treatment, interleukin-1 (IL-1), IL-6, tumor necrosis factor-α (TNF-α), high sensitive C-reaction protein (hs-CRP) were reduced significantly in both groups (P<0.05), and the levels in research group were all significantly less than those in control group (P<0.05). ④ Endothelial function:after treatment, endothelin, and von willebrand factor were reduced significantly in bith groups (P<0.05), and the levels in research group were significantly less than those in control group (P<0.05). Conclusion: Danhong injection can effectively improve the clinical symptoms of thrombotic shallow phlebitis, improve hemodynamics and endothelial function, and inhibit inflammatory reaction.关键词：thrombotic shallow phlebitis;hemodynamics;inflammation;endothelial function
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发布时间：2024-01-04 - 摘要：To explore the prescription rules of modern clinical traditional Chinese medicine(TCM) in the treatment of viral pneumonia, retrieve relevant clinical literature on TCM prescriptions for the treatment of viral pneumonia from domestic Chinese databases, such as CBM, CNKI, VIP, Wanfang data in recent 30 years, select prescriptions in accordance with the inclusion criteria, and extract high frequency drugs and high association drug combinations based on the frequency distribution and association rules in Traditional Chinese Medicine Inheritance Auxiliary System. The results showed that 119 kinds of herbs were present in the included 65 prescriptions, including 25 kinds of commonly used herbs, 33 commonly used core herb combinations (support degree 15%, confidence level 0.9). The results showed that the core herbs were Bitter Almond, Glycyrrhizae Radix et Rhizoma, Honeysuckle,Forsythia Suspense, and Gypsum in the treatment of viral pneumonia via antiviral and immune adjustment, with heat clearing, detoxifying, and relieving cough and asthma as the main treatment methods.关键词：viral pneumonia;Traditional Chinese Medicine Inheritance Auxiliary System;prescription rules
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发布时间：2024-01-04 - 摘要：Objective: To explore the prescription rules of modern Chinese medicine for external use in the treatment of ankylosing spondylitis by collecting the prescriptions in literature on external herbal medicines for ankylosing spondylitise, so as to provide reference for clinical medication. Method: Through the search of Chinese Academic Journal(CNKI) and Wanfang academic periodical full-text database from January 2004 to 2015 March, to collect, reorganize, summarize and the analyze the domestic clinical literature on relevant external application of traditional Chinese medicine(TCM) for treating ankylosing spondylitis, and explore the prescription rules of modern clinical Chinese medicine for external use in the treatment of ankylosing spondylitis by frequency analysis method. Result: In a total of 62 effective references, external Chinese medicine treatment for ankylosing spondylitis was mainly from the functions of expelling wind and removing dampness, activating blood circulation to dissipate blood stasis, dispelling cold and relieving pain, as well as nourishing the liver and kidney. The herbal properties of TCM were mainly warm and flat, flavor was mainly pungent and bitter, the meridian was mainly for liver, spleen and kidney. Conclusion: External TCM treatment has significant advantages on local efficacy and broad prospects for development compared with internal treatment. Though preliminary exploration on prescription rules of external Chinese medicine treatment for ankylosing spondylitis, we are expecting to find a more effective treatment method for ankylosing spondylitis and provide reference for clinical medicine.关键词：Chinese medicine external treatment;ankylosing spondylitis;drug analysis
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发布时间：2024-01-04 - 摘要：Objective: To systemically review the effect of ginkgo biloba extract (GbE) on microalbuminuria and renal function in patients with diabetic nephropathy (DN). Method: Literature were retrieved from PubMed, CNKI, VIP, CBM and Wanfang database to select the randomized controlled trials (RCT) where conventional treatment was as the control group, and GbE was also used on the basis of conventional treatment as the experimental group to study its effect on microalbuminuria and renal function in patients with diabetic nephropathy. RevMan 5.2 software was used for Meta analysis after 2 researchers selected the literature according to inclusion and exclusion criteria, extracted the data and evaluated the methodological quality in the study. Result: 16 RCTs were included, involving 1 212 patients. Meta-analysis showed that compared with control group, experimental group can significantly decrease urine albumin excretion rate (UAER)[SMD=1.00, 95%CI(0.70,1.30),P<0.01]and,24-hour urinary protein quantity[SMD=1.00, 95%CI (0.70, 1.30), P<0.01] and 24 h urine protein quantitation[SMD=1.00, 95%CI (0.70, 1.30), P<0.01], but can not significantly decrease serum creatinine (SCr)[SMD=0.11, 95%CI (-0.03, 0.26), P>0.05]. Conclusion: Ginkgo biloba extract can significantly reduce the microalbuminuria in patients with diabetic nephropathy, but has no significant improvement for renal function. Due to the restrictions of the quality of included studies, this conclusion needs to be further verified by large sample and high quality RCTs.关键词：ginkgo biloba extract (GbE);diabetic nephropathy (DN);microalbuminuria;renal function;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the efficacy and safety of Xiyanping combined with montmorillonite power in the treatment of infantile diarrhea. Method: literature were retrieved from Pubmed, Embase, Cochrane library, CNKI, CBM, Wanfang Data and VIP to select randomized clinical trials on Xiyanping combined with montmorillonite power for the treatment of infantile diarrhea. Rigorous quality evaluation and data extraction were done independently by two researchers, and treatment, RevMan 5.3 software was used for Meta-analysis. Result: 17 RCTs were eventually included, with a total of 1841 cases of children. Meta-analysis showed that:Xiyanping combined with montmorillonite power in the treatment of infantile diarrhea was statistically significant when compared with the control group intotal efficiency, the time of diarrhoea stop and recovery time of abnormal stool (P<0.05),but there was no statistically significant difference in time of body fever relieving between the two groups. Conclusion: Xiyanping combined with montmorillonite power has a certain effect and high safety in the treatment of infantile diarrhea, but with the poor quality of the existing studies, we need to design more rigorous and high quality multicenter, randomizeed, double-blinded experiments for verification.关键词：Xiyanping;montmorillonite power;infantile diarrhea;Meta-analysis
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发布时间：2024-01-04 - 摘要：Based on the data mining methods of association rules Apriori algorithm and complex system entropy clustering, 108 cream prescriptions for treating bronchial asthma by professor WU Yin-gen were collected and analyzed for the frequency of herbs usage and the association rules between herbs. 10 core combinations such as 'Xanthii Fructus-Rubiae Radix-Magnoliae Flos-Angelicae Dahuricae Radix' and 10 new prescriptions such as 'Arnebiae-Wartwort-Gigeriae Galli Endothelium Corneum-Belamcandae Rhizoma-Ramuli Euonymi-Polygonati Rhizoma' were extracted. Part of the prescription rules of the cream formula for bronchial asthma by professor WU Yin-gen were obtained, such as conditioning 'cold constitution' via tonifying spleen and kidney in different ways, taking seriously the related symptoms of allergic rhinitis and eczema, treating phlegm after distinguishing cold and heat,treating Qi movement via regulating mechanism of pivot,paying attention to dredging meridians and promoting blood circulation on asthma related with women' s menses and delivery.关键词：WU Yin-gen;bronchial asthma;traditional Chinese medicine cream formula;association rules;clustering algorithm
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发布时间：2024-01-04 - 摘要：Objective: To systemically assess the efficacy of the integrated traditional Chinese medicine(TCM) and western medicine for treatment of hashimoto's hypothyroidism. Method: The randomized controlled trials (RCTs) of the integrated TCM and western medicine as well as western medicine alone for treatment of Hashimoto's hypothyroidism were searched by electronic databases. All relevant literature were selected according to the inclusion criteria and data was extracted, then Stata10.1 software was used for statistic analysis. Result: The 13 randomized controlled trials were included in the study, with 1 017 patients in total. Compared with western medicine alone group, the observation group of integrated TCM and western medicine significantly improved the clinical efficacy[OR=3.89, 95%CI (2.71, 5.59), P<0.01]. In terms of TPOAb and TGAb, the observation group was significantly superior to western medicine alone group[SMD=-1.62, 95%CI(-2.29,-0.95), P<0.01;SMD=-2.96, 95%CI(-3.92,-1.99), P<0.01, respectively). Conclusion: The integrated TCM and western medicine is obviously superior to western medicine alone for treatment of Hashimoto's hypothyroidism, especially in improving TGAb and TPOAb.关键词：hashimoto's thyroiditis;hypothyroidism;integrated traditional Chinese medicine and western medicine;Meta-analysis
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发布时间：2024-01-04 - 摘要：Acute gouty arthritis (AGA) is a crystal joint disease caused by uric acid crystal deposition in the joints which was caused by purine metabolic disorder. By reviewing the literature reports in recent years on pharmacological effects and mechanism of natural products and their effective components against AGA, and focusing on the pathogenesis of urate crystal-induced acute gouty arthritis in animal models, we collected,summarized and analyzed the literature on targets and pharmacological action mechanism of natural products and their effective components against acute gouty arthritis. Studies have shown that the main effective components in natural products against acute gouty arthritisinclude flavonoids, alkaloids, polyphenols, saponins, and coumarin, etc. They fight against the acute gouty arthritis through anti-inflammatory, analgesic and inhibiting the release of inflammatory mediators as well as its related signaling pathways, antioxidant mechanism and multiple drug targets. Targets for the treatment of acute gouty arthritis are single in western medicine, with more serious side effects, thus not conducive to control the recurrence of acute gouty arthritis. Natural products in traditional Chinese medicines have a thousand years of history and experience in prevention and treatment of acute gouty arthritis. Compared with the western medicine, natural medicine has shown great potential and advantages of multiple targets and few side effects for the acute gouty arthritis. In recent years, more and more researchers use experimental pharmacology and molecular biology method to study the effect of natural products and their effective components against acute gouty arthritis, and have achieved gratifying results.关键词：acute gouty arthritis;nature products;active component;flavonoids;alkaloids;pharmacological studies
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发布时间：2024-01-04 - 摘要：Camellia nitidissima has attracted considerable attention as a rare and famous ornamental species characterized by yellow waxy petals in the genus Camellia of Theaceae. The flowers and leaves of C. nitidissima, a traditional herb in Chinese medicine, are commonly used for the treatment of faucitis, nephritis, hepatitis with jaundice, dysentery, diarrhea, hypertension, urinary tract infection and irregular menstruation. Studies have demonstrated that C. nitidissima mainly has flavonoid constituents, including quercetin,kaempferol and their glycoside Derivatives. In addition, C. nitidissima also has polyphenols, polysaccharides, saponins and volatile components. Both in vivo and in vitro pharmacological studies showed that C. nitidissima has anti-tumor, anti-oxidant, hypolipidemic, hypoglycemic, anti-allergy, anti-skin aging,anti-bacterial, and other pharmacological effects. The present paper presents the research progress of chemical composition and pharmacological effects of C. nitidissima in recent years, and provide theoretical evidence for the full development and utilization of C. nitidissima resources.关键词：Camellia nitidissima;chemical constituents;pharmacological activities
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