最新刊期
卷 22 ， 期 4 ， 2016
- 摘要：Objective: To optimize desalination process of ethanol supernate of Mactra veneriformis,and analyze its changes in chemical composition and safety. Method: Taking desalination rate,transfer rates of solid content and amino acids(in the amount of taurine and alanine) as comprehensive evaluation index,orthogonal test was adopted to optimize desalination process with the concentration of sample solution,pH of liquid and desalination time as factors.Composition changes of ethanol supernate of M. veneriformis before and after desalination was compared,and its safety was evaluated by acute toxicity test. Result: The best desalination process was as follows:sample solution concentration of 34 g · L-1,pH of 4-5 and desalination time of 1.5 h.With optimum process for desalinating,desalination rate was over 90%,transfer rate of solid content was more than 65%,retention rates of taurine and alanine were more than 90%.Lethal dose (LD50) of ethanol supernate before desalination was 27.18 g · kg-1,while after desalination with electrodialysis,the maximum tolerated dose increased to 37.48 g · kg-1. Conclusion: Toxic ingredients in ethanol supernate of M. veneriformis after desalination reduce significantly,but at the same time,effective components still remain and safety has improved significantly.Optimized desalination process is stable and feasible,it can provide theoretical basis for further research and development of M. veneriformis.关键词：Mactra veneriformis;desalination process;comprehensive score;acute toxicity test;desalination rate
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发布时间：2024-01-04 - 摘要：Objective: To optimize process of polydatin being transformed into resveratrol by response surface methodology for increacing the content of resveratrol and promoting resource development of Polygoni Cuspidati Rhizoma et Radix. Method: Considering yield of resveratrol as index,based on single factor experiments,response surface methodology was adopted to optimize transformation process by taking the amount of enzyme,temperature,pH and time as factors.UV was employed to determine the content of resveratrol at 305 nm. Result: Optimum technological conditions were as follows:enzyme dosage of 463 μL,temperature at 43 ℃,pH of 5.0,conversion time of 153 min;yield of resveratrol was 16.46 mg · g-1,whose deviation was small by comparing with the theoretical value of 16.52 mg · g-1,which was 3.58 times to before optimization and 1.66 times of the best enzyme hydrolysis process. Conclusion: Response surface analysis is suitable for optimizing transformation process of resveratrol,this process is feasible,which is a beneficial reference for reducing industrial production costs of resveratrol.关键词：Polygoni Cuspidati Rhizoma et Radix;polydatin;resveratrol;enzymolysis process;response surface analysis
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of compound Qiancao tablets. Method: Pharmacodynamic test was adopted to screen extraction method,orthogonal test was used to optimize conditions of extracting temperature,solvent amount and extracting time in extraction process of compound Qiancao tablets,with extracting rates of mollugin and hydroxynaphthoquinones as indexes.Molding process of compound Qiancao tablets was optimized by single factor tests. Result: Optimal extraction and molding processes were as follows:extracted twice with 10-fold and 8-fold 95% ethanol refluxing at 95 ℃,1 hour for per time,then vacuum dried to obtain extract;added MCC and CMS-Na into extract,mixed with 5% PVP-K30 as an adhesive to obtain wet granules,dried granules and granulated through a twenty-mesh sieve;added proper amount of magnesium stearate for tableting with coating weight of 1%.Dry extract rate was 4.57%,contents of mollugin and hydroxynaphthoquinones in extract were 6.68% and 10.81%.Hardness of the tablets was 4.5 kg and disintegration time was 25 min with good coating performance. Conclusion: These optimized processes are reasonably practicable,which can be applicable to industrial production of compound Qiancao tablets.关键词：compound Qiancao tablets;bleeding time;mollugin;adhesives;disintegration time
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发布时间：2024-01-04 - 摘要：Objective: To optimize ethanol extraction technology of Xinnaosu granules and provide a reference for molding technology of this preparation. Method: Based on single factor tests,taking overall desirability of extracting amounts of tanshinone ⅡA and cryptotanshinone,activity of anti-thrombin as index,ethanol concentration,the amount of ethanol and extraction time were selected by central composite design-response surface methodology,extraction technology was analyzed and verified by principal component analysis. HPLC was adopted to determine contents of tanshinone ⅡA and cryptotanshinone with isocratic elution of methanol-water (75 : 25) and detection wavelength at 270 nm. Result: Optimum extraction process conditions was as follows:extracted 120 min with 8 times the amount of 70% ethanol.Extracting amounts of tanshinone ⅡA and cryptotanshinone were 0.523,0.225 mg · g-1,activity of anti-thrombin was 92.599 U · g-1.Overall desirability deviated 2.9% by comparing with the predictive value. Conclusion: This optimized process is simple and reliable with high predictability,which can lay foundation for molding technology of Xinnaosu granules.关键词：Xinnaosu granules;anticoagulant activity;principal component analysis;Tanshinone ⅡA;Cryptotanshinone
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Qinghou Liyan buccal tablets,and provide references for its pilot production. Method: Based on pre-experiments,effects of soaking time,solvent dosage and extracting time on overall desibirability of contents of chlorogenic acid and totale flavonoids (included rutin,liquiritin and luteolin-7-O-glucoside) were investigated and forecasted by response surface metholodgy.HPLC was used to determine contents of chlorogenic acid and three flavonoids with mobile phase of acetonitrile-0.5% phosphoric acid for gradient elution and detection wavelengthes of 327 nm and 256 nm. Result: Optimal extraction process was as following:soaked 89 minutes and extracted twice with 15.3 times the amout of water for per time of 136 minutes.Concentrations of chlorogenic acid,luteolin-7-O-glucoside,rutin and liquiritin were 1 843.537,184.845,93.939,48.898 mg · L-1. Conclusion: This opimized extraction technology is stable and feasible with well prediction,it provides references for pilot production of Qinghou Liyan buccal tablets.关键词：Qinghou Liyan buccal tablets;chlorogenic acid;total flavonoids;luteolin-7-O-glucoside;rutin;liquiritin;comprehensive score
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发布时间：2024-01-04 - 摘要：Objective: To establish a quantitative analysis method for ferulic acid from Awei Haipiaoxiao soft capsules,which laid a test base for quality control of this preparation. Method: Independent variables were methanol concentration,solvent amounts and extracting time,dependent variable was peak area of ferulic acid,response surface methodology was adopted to optimize ultrasonic-assisted extraction technology,and an HPLC method for determining the content of ferulic acid from Awei Haipiaoxiao soft capsules was established. Result: Optimum extraction process was as following:adding 4.2 times the amount of 79% methanol,extraction time of 33 min.Conditions of HPLC were as follows:mobile phase of acetonitrile-0.1% phosphoric acid solution (15 : 85),flow rate of 1.0 mL · min-1,detection wavelength at 322 nm.The content of ferulic acid was 0.919 2 μg per grain. Conclusion: This quantitative analysis method of ferulic acid from Awei Haipiaoxiao soft capsules is simple,accurate and reproducible,which can be used for quality control of this preparation.关键词：Awei Haipiaoxiao soft capsules;ferulic acid;ultrasonic-assisted extraction;response surface methodology;Sepiae Endoconcha
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of processing on contents of segetalin A,B,E in Vaccariae Semen. Method: HPLC was employed to determine contents of segetalin A,B,E with AcclaimTM 120 C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile(A)-water(B) for gradient elution(0-8 min,32%A;8-13 min,32%-60%A;13-20 min,60%-70%A),flow rate of 1 mL · min-1,detection wavelength at 220 nm,column temperature at 40 ℃,injection volume of 10 μL.Changes of contents of segetalin A,B,E and dissolution rates were analyzed after Vaccariae Semen being processed. Result: After being processed,contents of segetalin A,B,E in processed products of Vaccariae Semen was similar to those of raw herbs,however,their dissolution rates raised 13.2%,5.3% and 17.4%,respectively. Conclusion: Processing has little influence on contents of segetalin A,B,E in Vaccariae Semen,but their dissolution rates increase.关键词：Vaccariae Semen;frying without adding anything;cyclopeptide;lactagogue effect;dissolution rate
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发布时间：2024-01-04 - 摘要：Objective: To conduct correlation analysis of mineral nutrient elements in soil and herbs of Paridis Rhizoma growing in different places around three gorges reservoir. Method: Rhizome and rhizosphere soil of Paridis Rhizoma were disposed with the wet digestion method, and then determined by atomic absorption spectrophotometry.Data were analyzed and compared with SPSS 18.0. Result: Nutrient elements in Paridis Rhizoma and rhizosphere soil showed some correlation.There were significant differences existing in concentrations of eight kinds of nutrient elements in rhizome and rhizosphere soil of Paridis Rhizoma from different places.Contents of each nutrient elements in ranking of rhizome was K> Ca> Na> Mg> Cu> Mn> Ni> Zn,and that in rhizosphere soil was Mg >Na >Ca >K >Mn> Zn >Ni> Cu.Paridis Rhizoma had a certain capability of enriching Na,Cu and Ca.Eight kinds of nutrient elements in rhizome and rhizosphere soil had multiple correlation. Conclusion: Main regulation pathway of mineral nutrient elements in Paridis Rhizoma is selective absorption.Coordination among nutrient elements is in favor of accumulation of nutrient elements in Paridis Rhizoma.关键词：three gorges reservoir;Paridis Rhizoma;soil;nutrient elements;enrichment factor
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from Aconitum tanguticum. Method: The chemical constituents were isolated and purified by using a combination of modern chromatographic techniques, and their structures were determined through spectral analysis(1D, 2D-NMR and MS) and literature comparison. Result: Seventeen compounds were isolated and identified as isolumichrome(1), p-hydroxycinnamic acid(2), benzoic acid(3), p-hydroxy phenylethylamine(4), p-hydroxy phenylethanol(5), butylparaben(6), adenosine(7), 3-[p-hydroxyphenyl]-propionic acid amide(8), hetisine(9), guanfu base H(10), salidroside(11), cyclo-(ile-leu)(12), phenylalanine(13), 3-(4-aminophenyl)prop-2-enoic acid(14), N-oxide hordenine(15),(2R,3R)-2,3,4-trihydroxy-butanoic acid(16), and N-methyltyramine(17). Conclusion: Compounds 1-4, 6-8 and 12-17 were isolated from A. tanguticum for the first time.关键词：Aconitum tanguticum;chemical constituents;water-soluble alkaloids
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发布时间：2024-01-04 - 摘要：Objective: To preliminarily interpret the compatibility of ginseng and flower of Pueraria lobata in chemical and pharmacodynamical levels, thus provide theoretical basis for its clinical application. Method: Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry(RRLC-Q-TOF-MS) has been applied to identify and analyze the chemical components of the flower of Pueraria lobata and Ginseng Radix et Rhizoma before and after compatibility. 0.1% formic acid water (A)-acetonitrile (B) was used as the mobile phase for gradient elute (0-12 min, 15%B;12-20 min, 15%-50%B). The flow rate was set to 0.4 mL · min-1 and the sample injection volume was 5 μL. Principal component analysis (PCA) was used to find the differences in chemical components before and after compatibility, and pharmacodynamical experiment was conducted for the compatibility of these two herbs. Result: The chemical components of flower of Pueraria lobata and Ginseng Radix et Rhizoma were identified before and after the compatibility;in negative ion mode of mass spectrometry, irisolidon, 6"-O-xylosyl-glycitin, ginsenoside Rg1, and ginsenoside Rf contents in the compatibility mixture were significantly increased. By PCA analysis, the components before and after compatibility can be clearly distinguished. In the enzymatic activity assay, flower of Pueraria lobata had the acceleration effect for enzyme reaction, and three indicators had significant changes during the in vivo experiments. Conclusion: Compatibility of Ginseng Radix et Rhizoma and flower of Pueraria lobata has significant effect on alleviating hangover and protecting liver in vitro and in vivo, and the content and changes of chemical components have been detected, providing theoretical basis for its clinical application and development.关键词：flower of Pueraria lobata;Ginseng Radix et Rhizoma;liquid chromatography-mass spectrometry;alleviating hangover and protecting liver
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发布时间：2024-01-04 - 摘要：Objective: To study on the effect of different processing methods on the curcuminoids content in Curcumae Longae Rhizoma and Curcumae Radix from different areas, and thus provide reasonable reference data for its processing technology. Method: The samples were processed by shade-drying after slicing, sun-drying after boiling and oven-drying under 60 ℃ and 80 ℃, then the contents of curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcumae Longae Rhizoma and Curcumae Radix were simultaneously determined by HPLC method. Result: The curcuminoids content in Curcumae Longae Rhizoma had significant differences between different processing methods (P<0.05), and the content was in the following order from high level to low level:shade-drying after slicing> oven-drying under 60 ℃> oven-drying under 80 ℃> sun-drying after boiling. There were also significant differences in curcuminoids content in Curcumae Radix by different processing methods (P<0.05), and the content was in the following order from high level to low level:shade-drying after slicing> oven-drying under 60 ℃> sun-drying after boiling> oven-drying under 80 ℃. Curcumae Longae Rhizoma and Curcumae Radix from Qianwei area showed higher content of curcuminoids than Chongzhou area, with extremely significant difference (P<0.01). Conclusion: Different processing methods have great effects on the content of curcuminoids in Curcumae Longae Rhizoma and Curcumae Radix, and shade-drying after slicing is a best processing method for Curcumae Longae Rhizoma and Curcumae Radix.
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发布时间：2024-01-04 - 摘要：Objective: To identify and analyze the infrared fingerprints of fruits of Juglans mandshurica samples from different areas of Heilongjiang province. Method: One-dimensional IR spectrum combined with the second derivative spectrum, as well as similarity comparison software were used to process the IR fingerprints of fruits of J. mandshurica from 13 origins and their main active ingredient juglone. Result: The fingerprint characteristics of fruits of J. mandshurica were identified and their difference between different origins were studied. The content of chemical composition in fruits of J. mandshurica was higher in Jixian county, Tangyuan county, and Hailin, but lower in Harbin and Huanan county. Conclusion: For fruits of J. mandshurica in different origins, infrared fingerprint analysis is an effective and quick method for identification and analysis.关键词：fruits of Juglans mandshurica;IR;second derivative spectrum;identification
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发布时间：2024-01-04 - 摘要：Objective: To establish reversed phase ion pair chromatography method for the determination of two atractyloside toxicants in Xanthii Fructus. This method will shorten the analysis time and improve specificity compared with the former methods. Method: The chromatographic separation was carried out on Agilent Eclipse XDB-C18 column (4.6 mm×250 mm, 5.0 μm). The mobile phase was composed of acetonitrile-0.12% tetrabutylammonium hydroxide in 0.15% sodium dihydrogen phosphate solution (pH of phosphoric acid was adjusted to 3.5) (39 : 61). The flow rate was set at 1.0 mL · min-1 and the column temperature was 30 ℃, with detection wavelength of 203 nm. Result: Under the above conditions, two toxicants could be well separated in 20 min (11.8,17.8 min, respectively). The actual sample size had good linear relationship within the range of 0.15-3.7 μg (r=0.999 9) and 0.10-2.5 μg (r=1.000),respectively. The average recovery rate was 101.4% (RSD 1.7%) and 101.6% (RSD 1.2%),respectively. Conclusion: This method is quick, accurate, specific and durable, which can be used for safety detection of Xanthii Fructus herbs and their preparations.关键词：Xanthii Fructus;reversed phase ion pair chromatography;carboxyatractyloside;atractyloside;toxic ingredient
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发布时间：2024-01-04 - 摘要：Objective: To found a quantitative HPLC-ELSD method for determining tenuifoliside A,1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside and ginsenoside Rb1 from Kaixin San,which are used for anti-tumor. Method: The procedure of HPLC-ELSD was performed on the chromatographi column of Agilent HC C18 (4.6 mm×250 mm,5 μm)at 25 ℃, and the mobile phase was acetonitrile-0.65% ammonium acetate solution in gradient elution.The flow velocity was 1.0 mL · min-1.The temperature of driti tube was 115 ℃ and the gas was air, flow rate was 3.2 L · min-1. Result: Tenuifoliside A showed a good linear relationship at a range from 1.113 μg to 10.980 μg, 1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside,from 1.581 μg to 15.726 μg,and ginsenoside Rb1,from 0.712 μg to 7.128 μg;Precision,stability and repeatability are comply with the requirement of quantitative analysis. The average recovery was respectively 98.36%,99.58% and 98.97%.The percentage composition was respectively 0.999%,1.670% and 0.579%. Conclusion: The method was simple and reliable,which can be used for the assaying of tenuifoliside A, 1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside and ginsenoside Rb1 as well as quantity control of Kaixin San.关键词：Kaixin San;oligosaccharide esters;ginsenoside Rb1;HPLC-ELSD;anti-depression
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发布时间：2024-01-04 - 摘要：Objective: To determine the major components of volatile oil from Forsythiae Fructus in different origins by gas phase method, and compare its antibacterial activity in vitro. Method: Volatile oil of 16 batches of Forsythiae Fructus in different origins were extracted by steam distillation method;the major components and content were detected and analyzed by gas chromatography (GC);their antibacterial activity was analyzed and compared by K-B disk diffusion method. Chromatographic conditions:HP-5 capillary chromatographic column (0.25 mm×30 m, 0.25 μm). Procedure temperature:initial temperature of 50 ℃, and increased to 86 ℃ at the rate of 5 ℃ ·min-1, kept for 3 min, and then increased to 220 ℃ at the rate of 20 ℃ ·min-1, and kept for1 min. Injection port temperature was 230 ℃, and detector temperature was 250 ℃. Result: α-pinene, camphene, β-pinene, myrcene, p-cymene, limonene, and α-terpineol had good linear relationship within the range of 0.016 5- 0.988 8, 0.005 0-0.300 0, 0.038 1-2.286 0, 0.003 2-0.192 0, 0.000 8-0.048 0, 0.008 2-0.492 0 and 0.002 4 -0.144 0 μg(r≥ 0.999 7) respectively;RSDs of the stability test and reproducibility test were less than 3.0%;the recovery rate of the method was in the range of 99.0%-103.4% (RSD<3.0%);there were differences in the contents of the major components of volatile oil from Forsythiae Fructus in different origins, and the volatile oil had different inhibitory effect against five kinds of the tested strains. Conclusion: The established GC method is simple, accurate and reliable, and can be used for quality evaluation and antibacterial activity evaluation of Forsythiae Friuctus.关键词：Forsythiae Fructus;volatile oil;GC;antibacterial activity
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of different decoction methods on the quality of Shenqi Yangxue preparations, and provide the evidence for choosing the preparation method of tonifying recipes. Method: Ratio of extraction, n-BuoH extract content and astragaloside Ⅳ content were used as the evaluation indexes. UPLC-Q-TOF-MS method was adopted to determine the astragaloside Ⅳ content. ACQUITYTM C18(2.1 mm×50 mm,1.7 μm)column was used with acetonitrile-0.1% formic acid water as the mobile phase for gradient elution. The flow rate was set to 0.45 mL · min and the column temperature was 40 ℃. The mass spectra were obtained by Xevo G2 Q-TOF with electrospray ionization source in negative ion mode to determine the content of astragaloside IV[M＋COOH]-,m/z 829.45. Result: astragaloside Ⅳ had good linear relationship within the range of 9.8×10-5-9.8×10-3 μg(r=0.999 0)with an average recovery rate of 100.32%.The astragaloside IV contents of 3 kinds of Shenqi Yangxue preparations were 0.193-1.976 mg/dose, with total solid weight of 31.297-103.077 g/dose and n-BuoH extract content of 6.11-14.55 g/dose. Conclusion: 3 preparation methods had significant difference in ratio of extraction, n-BuoH extract content and astragaloside IV content, so the preparation process had great effect on the evaluation indexes. The preparation method by vacuum concentrated machine had the best effect.关键词：Shenqi yangxue preparation;astragaloside IV;solid content;n-butyl extract content;LC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a method of quantitative analysis of multi-components by single marker (QAMS) for the determination of L-hydroxyproline, glycine, alanine and proline in Asini Corii Colla. Method: With L-hydroxyproline as the internal reference substance, the relative correction factors (RCF) of glycine, proline and alanine were determined by HPLC and were used to calculate corresponding contents.At the same time, the contents of the four amino acids were determined by external standard method, and the calculated values and estimated values were compared to ensure the accuracy and feasibility of QAMS. Result: The experiments showed good linear relationship at the determination ranges: L-hydroxyprolinem 0.017-0.26 μg (r=0.999 8), glycine 0.033-0.50 μg (r=0.999 7), alanine 0.015-0.23 μg (r=0.999 5) and proline 0.025-0.37 μg (r=0.999 8). The average recoveries of four componts were from 96.8% to 98.4% and the relative standard deviations were all less than 3.0%. The RRT of glycine, alanine and proline were 1.386,1.940 and 2.129, and the RCF were 0.642,0.975 and 0.951, respectively. Calculated values of QAMS and estimated values of external standard method did not make significant difference. It showed that the RCF was authentic. Conclusion: QAMS has the applicability and feasibility for the determination of the four amino acids ingredients in Asini Corii Colla.关键词：quantitative analysis of multi-components by single-marker (QAMS);relative correction factors (RCF);Asini Corii Colla;amino acid
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Qingre Huoxue Jiedu complex prescription (QHJD) on the apoptosis and proliferation of the tumor necrosis factor-α(TNF-α) induced HaCat cell in vitro, in order to provide basis for revealing potential mechanism for treating psoriasis. Method: TNF-α-induced HaCat cells in vitro were cultured and exposed to the crude extract of QHJD with low, middle and high concentrations. Morphological changes were observed under inverted microscope. Cell proliferation was detected by CCK-8 method. Cell apoptosis was analyzed by flow cytometry. Expression levels of Bcl-xl and Bax protein of HaCat cell was detected by Western blot method. Expression levels of Bcl-xl and Bax mRNA expressions of HaCat cell was detected by Real-Time PCR. Result: Compared to the blank group, the QHJD groups showed a reduction in the number of cells per unit area under the microscope and cells volume, decrease or disappearance of connections between cells, and enlargement in intercellular space;all the QHJD groups can inhibit the HaCat cells proliferation (P<0.05), with statistically significant difference (P<0.05). Specifically, and the high-dose group can inhibit the HaCat cells proliferation obviously (P<0.01);the QHJD groups can increase the expression level of Bax protein, with statistically significant difference (P<0.05);the QHJD groups can increase the expression level of Bax mRNA, with statistically significant difference (P<0.05). Conclusion: QHJD might inhibit the TNF-α-induced HaCat cells proliferation and treat psoriasis by up-regulating the expression of Bax protein and mRNA and inducing apoptosis.关键词：Qingre Huoxue Jiedu complex prescription;psoriasis;HaCat;tumor necrosis factor-α;proliferation;apoptosis;Bcl-xl;Bax
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发布时间：2024-01-04 - 摘要：Objective: To study major functional components of Prunellae Spica and its protective effect against the oxidative injury induced by acute restraint stress in mice. Method: The male Balb/C mice were randomly divided into negative group and model control group, and 1.25, 2.50, 7.50 g · kg-1 Prunellae Spica intervention groups. Mice in the intervention groups were given Prunellae Spica by gavage for 5 days, mice in model control group and negative group were given distilled water. After the last administration, intervention groups and model control group received 2 h acute restraint stress, and then the mice in the groups were sacrificed. The following anti-oxidative indicators in brain tissues were detected respectively:oxidative stress reactive oxygen species (ROS), hydrogen peroxide (H2O2), malondialdehyde (MDA), 8-hydroxy-deoxyguanosine (8-OHdG), protein carbonyl content, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity. We analyzed main components of prunella, including total flavonoids (aluminum nitrate chromogenic method), total triterpenoids (vanillin colorimetric method) and total polysaccharides (phenol-sulfuric acid method), and detected their total antioxidant capacity (ORAC). Result: Compared with the negative group, content of H2O2,MDA, 8-OHdG and protein carbonyl in model group significantly increased, while SOD activity obviously decreased (P<0.05, P<0.01). No obvious difference was found in ROS and GSH-Px contents. Compared with the model control group, SOD activity in the three intervention groups was increased (P<0.05, P<0.01), content of H2O2, MDA and protein carbonyl were much decreased. Total flavonoids content was 8.91 mg · g-1, total triterpene content was 2.45 mg · g-1, and total polysaccharide content was 10.16 mg · g-1. Total ORAC values of total flavonoids, total triterpenoids and polysaccharides were (127.5±1.0), (45.75±2.5), (2.25±0.75) mmol · L-1,respectively. Conclusion: Prunellae Spica intervention showed a certain protective effect against the oxidative injury induced by acute restraint stress in mice, and flavonoids may be the major functional ingredient against oxidative stress.关键词：Prunellae Spica;restraint stress model;flavone
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of Qiwei Baizhu San, Qiwei Baizhu drops and Qiwei Baizhu extract on diarrhea, intestinal pathological damage and tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in serum of mice with intestinal flora. Method: Ceftriaxone, lincomycin hydrochloride and iced senna were gavaged to mice to induce the intestinal flora model. The mice were divided into 5 groups:normal group, model group (natural recovery group after successfully modeling), Qiwei Baizhu San group, Qiwei Baizhu drops group and Qiwei Baizhu extract group. Analysis was made for diarrhea, small intestinal epithelial histopathology and the content of TNF-α and IL-6 in serum after treatment. Result: Compared with the normal group, the quantity of Enterococci, Escherichia coli, Lactobacillus and Bifidobacterium bacteria in the model group increased significantly (P<0.05, P<0.01), indicating the successful modeling. Different formulated Qiwei Baizhu San can reduce the number of diarrhea in animal models, lower degree of diarrhea, the rate of diarrhea and diarrhea index, relieve the small intestinal pathological damage and reduce the level of TNF-α and IL-6 in serum. But the decoction group showed a lower effect than the drop group and the extract group. Conclusion: Qiwei Baizhu San can correct diarrhea of mice with intestinal flora, and its mechanism may be correlated with promotion of the injured intestinal mucosa injury and repair and reduction of the release of inflammatory cytokines.关键词：Qiwei Baizhu San;diarrhea;intestinal flora;intestinal mucosa;inflammatory cytokines
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发布时间：2024-01-04 - 摘要：Objective: To investigate the regulatory effect of Tanyu Tongzhi (TYTZ) recipe on autophagy and antiapoptosis gene of myocardial ischemia-reperfusion injury in rats. Method: Totally 80 SD rats were randomly divided into sham-operated group, model group, 3-MA intervention (13.5 mg · kg-1 iv) and TYTZ recipe (43 g · kg-1 ig) groups. The model of ischemia and reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min and reperfusion for 2 hours in rats. Serum contents of CK, LDH were measured by automatic biochemical analyzer. The expression level of Beclin-1, LC3 and Bcl-2 were determined with RT-PCR. Result: Compared with the sham-operated group, the contents of CK, LDH and the expression of Beclin-1, LC3 mRNA were both significantly increased, while the level of Bcl-2 mRNA were decreased in MI and IR model groups (P<0.01). Compared with the model group, the contents of CK and LDH in ischemic period and reperfusion period, the expression of Beclin-1 mRNA in ischemic period and Beclin-1, LC3 mRNA in reperfusion period were lower in TYTZ recipe groups, and myocardial Bcl-2 mRNA activities of the groups in ischemic period and reperfusion period were increased (P<0.05,P<0.01). Compared with the sham-operated group and 3-MA group, the expression of Beclin-1, LC3 mRNA in ischemic period were increased in TYTZ recipe groups (P<0.01). Beclin-1 expression was found to have negative correlation with Bcl-2 expression of time in ischemia and reperfusion periods by Pearson test (P< 0.01). Conclusion: The TYTZ recipe can reduce myocardial ischemia/reperfusion injury by promoting the occurrence of autophagy during ischemia period, inhibiting them in reperfusion and promoting the expression of the anti-apoptotic protein Bcl-2.关键词：myocardial ischemia-reperfusion injury;autophagy;antiapoptosis;tanyu tongzhi recipe
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Shen'an granule on bone metabolism in 5/6 nephrectomy model rats. Method: Renal osteodystrophy (ROD) rat models were established using 5/6 nephrectomy. The rats were randomly divided into normal group, sham operation group, model group, positive drug calcitriol group (1.5 μg · kg-1), Shen'an granules low dose group and high dose group (2.25, 4.5 g · kg-1). Corresponding drugs were given by gavage for 8 weeks. Before and after treatment, blood urea nitrogen (BUN), serum creatinine (SCr), hemoglobin (HGB), calcium (Ca), phosphor (P), and alkaline phosphatase (ALP) were detected;intact parathyroid hormone (iPTH) was detected with chemiluminescence method, and bone density was measured with dual energy X ray. Result: Compared with the normal group, BUN and SCr levels of ROD model rats were significantly increased (P<0.05), HGB, serum Ca and bone density levels were significantly decreased (P<0.05), serum P, ALP and whole iPTH levels were significantly increased (P<0.05). Compared with the model group, BUN and SCr levels in Shen'an granule 2.25, 4.5 g · kg-1 dose groups were significantly decreased (P<0.05), HGB, serum Ca and bone density levels were significantly increased(P<0.05), serum P, ALP and whole iPTH levels were significantly decreased (P<0.05). Conclusion: Shen'an granule can significantly improve the renal function and bone metabolism indexes in 5/6 nephrectomy model rats, and delay progression of renal function and reduce the complications.关键词：Shen'an granule;5/6 nephrectomy;bone metabolism
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect and mechanism of Xuefu Zhuyu Tang on the unpredictable stimulus-induced blood stasis. Method: SPF grade rats were randomly divided into normal group, model group, Xuefu Zhuyu Tang high dose group and low dose group. Multi-factors combined stimulation method was used for 5 weeks to create blood stasis syndrome models, and at the same time, 105.3, 35.1 g · kg-1 Xuefu Zhuyu Tang was given by gavage. Multi-factors stimulation method was used to establish the animal models of ‘qi stagnation and blood stasis syndrome’, ‘flying spot’ method was used to dynamically simulate blood flow velocity in microcirculation, laser doppler technology was used to detect blood flow volume of the tissues, coagulation method was used to detect the blood coagulation, spectrophotometric method was used to determine plasma nitric oxide (NO), and nitric oxide synthase (NOS) activity, reverse transcription-polymerase chain reaction RT-PCR method was used to detect the expression of NOS mRNA. Result: Compared with the normal group, rate of weight gain in rats of the model group was lower than that in the normal group. Compared with the model group, Xuefu Zhuyu Tang could improve the condition of slow growth rats for body weight (P<0.05). Compared with the normal group, the arteriole and venule blood flow rates were significantly decreased in the model group, and the arteriole and venule vessel diameters were significantly contracted (P<0.05). Compared with the model group, the rats in treatment groups could significantly increase the mesenteric arteriole and venule blood flow rates, and significantly dilate the arteriole and venule vessel diameters (P<0.05), significantly increase the blood flow volume of skin, liver, and intestine (P<0.05), significantly reduce fibrinogen levels (P<0.05), and significantly increase the blood flow volume of liver, intestine, stomach, ovarian and uterine (P<0.05). Compared with the normal group, NO content in plasma of rats in model group was significantly lower(P<0.01), liver total NOS(TNOS) activity, constitutive NOS(cNOS) activity and endothelial NOS(eNOS) gene expression levels were significantly lower(P<0.01). Compared with the model group, NO levels in plasma (P<0.01), liver TNOS activity, cNOS activity and eNOS gene expression levels were significantly higher in the treatment groups (P<0.05). Conclusion: Xuefu Zhuyu Tang can improve the symptoms of blood stasis caused by unpredictable stimulus, and its action mechanism may be associated with the gas signal molecule NO.关键词：Qi stagnation and blood stasis;animal model;Xuefu Zhuyu Tang;nitric oxide;nitric oxide synthase
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Guiling Pa'an pill on the expression of Nestin, Nestin mRNA and dopamine transporter (DAT) in substantia nigra cells of rats with Parkinson's disease (PD). Method: SPF grade rats were stereotaxically injected with 6-hydrox-ydopamine (6-OHDA) and apomorphinum (APO) to induce PD rat models. Rats were randomly divided into model group, madopar group, Guiling Pa'an pill high dose group, middle dose group and low dose group, and at the same time, the sham operation group was also established. Rats in model group and sham operation group daily received the same volume of normal saline. Rats in madopar group received 10 mg · kg-1 · d-1 suspension by ig. Rats in Guiling Pa'an pill high dose group, middle dose group and low dose group received 4, 2, 1 g · kg-1 · d-1 suspension respectively by ig, all with a course of 30 days. The Nestin and DAT protein levels of the substantia nigra tissue were detected by immunohistochemistry for various groups;Nestin mRNA level in substantia nigra tissues was detected by hybridization in situ method for various groups. Result: Nestin and DAT protein expression and Nestin mRNA expression in substantia nigra tissues of the model group were significantly lower than those in sham operation group (P<0.01). Compared with model group, the Nestin protein, Nestin mRNA and DAT protein expressions in substantia nigra tissues of various treatment groups were significantly increased (P<0.01). Conclusion: Action mechanism of Guiling Pa'an pill for the treatment of Parkinson's disease might be associated with increasing Nestin, DAT expressions, which would promote the repair and regeneration of nerves and increase the content of dopamine.关键词：Guiling Pa'an pill;Parkinson's disease;Nestin;dopamine transporter
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发布时间：2024-01-04 - 摘要：Objective: To study the regulatory mechanism of Wenjing Xiaocuo granules on sex hormone in an animal experiment. Method: Totally 40 SD rats were randomly divided into blank group, tanshinone group (0.4 g · kg-1 · d-1), Wenjing Xiaocuo granules of low, medium and high dose groups (0.4 g · kg-1 · d-1), with 8 rats in each group. Wenjing Xiaocuo granules low, medium and high dose groups and tanshinone group were gavaged corresponding Wenjing Xiaocuo granules solution and tanshinone solution, blank group was gavaged distilled water for 7 days. Seven days later, efforts were made to take blood from the abdominal aorta, and cut off the uterus and weigh it, then calculate the coefficient of uterus and observe uterine pathology. IHC was used to detect the changes of estrogen receptor α (ERα) and estrogen receptor β (ERβ), ELISA was used to detect the content of estradiol (E2) and testosterone (T) in the rats. Result: In all of treatment groups, uterus coefficient increased, particularly the Wenjing Xiaocuo granules medium and high dose group (P<0.01). In all of treatment groups, uterine tissue hyperplasia was obvious, epithelial monolayer became stratified, the epithelial thickness also increased significantly. With respect to E2 level, compared with the blank group, all other groups were significantly higher (P<0.05, P<0.01), particularly Wenjing Xiaocuo granules high dose group. The content of T in high dose group was the lowest (P<0.01). Compared with blank group, the expressions of ERα and ERβ of all other groups were significantly increased (P<0.01), and the expressions of all Wenjing Xiaocuo granules groups increased with the rise in dose. Conclusion: Wenjing Xiaocuo granules can promote the secretion of estrogen, suppress the secretion of androgen, and increase the expression of estrogen receptor, with the effect enhancing with the increasing dose.关键词：Wenjing Xiaocuo granules;estrogen receptor;testosterone;estradiol
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Yishen Jiangzhuo Huayu decoction and disassembled formulas on expression of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) in rats with adenine-induced renal interstitial fibrosis, and explore the mechanism of Yishen Jiangzhuo Huayu decoction on renal interstitial fibrosis. Method: Totally 70 SPF male SD rats were divided into normal group, model group, Niaoduqing group, whole prescription group, disassembled formulas group 1, group 2, and group 3.All the rats except normal group were given intragastrically with adenine to produce renal interstitial fibrosis models. The normal group and model group received normal saline solution (10 mL · kg-1 · d-1), Niaoduqing group (2.5 g · kg-1 · d-1), whole prescription group (20.2 g ·kg-1 ·d-1), the disassembled formulas groups 1, 2, 3 (11, 8.2, 7.9 g · kg-1 · d-1). 4 weeks after administration, enzyme-linked immunosorbent assay (Elisa method) was used to detect TNF-α, IL-8 content in serum, and immunohistochemistry assay was used to detect TNF-α, IL-8 protein levels in renal tissues. Result: Compared with the normal group, TNF-α and IL-8 levels in serum and renal tissues were significantly higher in model group(P<0.05). Compared with the model group, TNF-α and IL-8 levels in serum and renal tissues were significantly lower in the whole prescription group, disassembled formulas group 1, group 2 and group 3 (P<0.05);TNF-α, IL-8 protein expression in renal tissues as well as TNF-α and IL-8 levels in serum of the whole prescription group were significantly lower than each disassembled formula group (P<0.05). Conclusion: Yishen Jiangzhuo Huayu decoction whole prescription group, disassembled formula group 1, group 2, and group 3 could down-regulate TNF-α, IL-8 expression, where the whole prescription group had the most significant effect.关键词：Yishen Jiangzhuo Huayu decoction;disassembled formula;renal interstitial fibrosis;tumor necrosis factor-α;interleukin-8
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发布时间：2024-01-04 - 摘要：Objective: To study the treating effect of Huangqi Guizhi Wuwu Tang in senile mouse with pruritus disease, and its possible mechanism from the perspective of immune imbalance. Method: Totally 84 Kunming mice were randomly into the normal group, the skin aging group, the skin aging pruritus model group, the diphenhydramine group (0.65 mg · kg-1), and low, middle and high-dose Huangqi Guizhi Wuwu Tang group (3.25, 6.5, 13 g · kg-1), with 12 mice in each group. Except for the normal group, all of the remaining groups were injected with D-galactose 125 mg · kg-1 through mouse neck and back for consecutively 42 days to build the senile mouse model;based on that,the senile pruritus model was induced by injecting dextran 1.25 mg · kg-1 through mouse caudal vein. Seven days later, mouse's scratching frequency and the scratching latency time of were observed with digital video camera. Then the content of interleukin-1β(IL-1β), IL-4, protease-activated receptor-2 (PAR-2) and substance P in serum were determined the enzyme linked immunosorbent assay, and the mast cells number in skin was counted under microscope. Result: Compared with the normal group, the skin pruritus group and skin aging pruritus model group showed signficiant decreases in scratching latency time and PAR-2 conent and increases in scratching frequency and serum IL-1β, PAR-2 and substance P expression (P<0.05). Huangqi Guizhi Wuwu Tang can significantly extend the latent scratching time (P<0.05) and decrease the scratching frequency (P<0.05). After intragastric administration, contents of IL-1, PAR-2 and substance P in serum of drug-treated mouse groups were lower than that of the skin aging pruritus model group (P<0.05), while IL-4 level in serum was significantly higher than the senile pruritus mouse model group (P<0.05). Meanwhile, mast cells number is decreased (P<0.05) compared with the senile pruritus mouse model group. Conclusion: Huangqi Guizhi Wuwu Tang has prominent therapeutic effect in senile pruritus, and its mechanism is correlated with the body's function of regulating immune imbalance.关键词：Huangqi Guizhi Wuwu Tang;senile pruritus;scratching frequency;immune imbalance
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发布时间：2024-01-04 - 摘要：Objective: To explore the hepatoprotective effects and the possible mechanism of 50% ethanol elute of Phlomis younghunsbandii by macroporous adsorptive resins (EPY-50) on CCl4-induced acute liver injury rats. Method: Sixty Wistar rats were randomly divided into normal group, model group, bifendate group (bifendate dropping pill, 100 mg · kg-1) and EPY-50 high-dose, medium-dose and low-dose (400, 200, 50 mg · kg-1) groups, continuous for 4 days by gavage. The rats in normal group and model group were given with distilled water. 1 hour after administration on day 3, rats were ip injected with 15% CCl4 olive oil;rats in normal group were ip injected with with olive oil, After contamination for 2 h and 22 h, all groups were administered again and blood was taken from orbital vein at 1 h after the last administration. The levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), and superoxide dismutase (SOD) were detected, then the rats were sacrificed for and livers were taken. The liver index and MDA, SOD, and glutathione reductase (GSH) levels in the liver tissues;in addition, histopathological examination was also done for the livers. Result: Compared with the normal group, serum AST and ALT levels were significantly elevated in model group (P<0.01), MDA levels were significantly increased (P<0.05), SOD and GSH levels were statistically reduced in the liver tissues (P<0.05), the liver index was significantly increased (P<0.01), indicating that the model of CCl4 injection was successful. Compared with the model group, EPY-50 with high-dose, and medium-dose (400, 200 mg · kg-1) could significantly decrease the levels of AST, ALT (P<0.01, P<0.01), significantly decrease MDA level, increase the SOD and GSH levels, reduce liver index and improve pathological state of the liver tissues. Conclusion: EPY-50 has hepatoprotective effects on CCl4-induced acute liver injury, and the mechanism may be associated with its antioxidative activity.关键词：Phlomis younghunsbandii;macroporous adsorptive resins;hepatoprotective effect
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发布时间：2024-01-04 - 摘要：Objective: To study the possible mechanism of the alcohol extract from gecko (GAE) on inducing prostate cancer cell line PC-3 apoptosis and provide theoretical foundation for the therapy of hormone independent prostate cancer. Method: The drug was extracted from gecko by alcohol. MTT assay was used to detect the growth inhibition effects of GAE on PC-3 cells of AIPC. With prostate cancer (PC)-3 cells as the research object, MTT method was used to detect the inhibition effect of 3.5, 4.0, 4.5, 5.0, 5.5 g · L-1 GAE after 24, 48 and 72 h on hormone dependence after prostate cancer (PC)-3 cell proliferation;after 48h treatment by 3.5, 4.0, 5.0 g · L-1 GAE, a blank group was established. Apoptosis-inducing effect was detected by Hoechest 33342 staining and Annexin V-FITC/PI double stained flow cytometry. The protein expression of Caspase-3 and Fas in PC-3 cells was measured by SP immunohistochemistry assay. Result: The proliferation of PC-3 cells treated with GAE (3.5, 4.0, 4.5, 5.0,5.5 g · L-1) for 24, 48, 72 h, respectively, was significantly inhibited in dose-and time-dependent manner. Compared with the blank group, after 48 h treatment by 3.5, 4.0, 5.0 g · L-1 GAE, typical apoptotic morphological changes were found in part of PC-3 cells by Hoechest 33342 staining. Annexin V-FITC/PI double stained flow cytometry showed that the early apoptotic cells account for 6.51%, 12.48% and 22.81% respectively after 48 h treatment by 3.5, 4.0, 5.0 g · L-1 GAE. Immunohistochemistry assay showed that the protein expression of Caspase-3 and Fas was increased in a concentration-dependent manner (P<0.05). Conclusion: GAE can induce prostate cancer cell line PC-3 apoptosis and the mechanism of action may be associated with the signal pathway mediated by death receptor.关键词：alcohol extract from gecko;PC-3 cells;apoptosis;fas;cysteine protease
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发布时间：2024-01-04 - 摘要：Objective: To observe the apoptosis mechanism of Banxia Xiexin Tang on esophageal carcinoma Eca9706 cells. Method: Flow cytometry technique was used to detect the effect of Banxia Xiexin Tang on cell cycle and apoptosis of esophageal carcinoma Eca9706 cells;Western blot was used to detect the effect of Banxia Xiexin Tang on signal transducer and activator of transcription 3 (STAT3) protein expression of Eca9706 cells. Result: After 24 h of treatment by Banxia Xiexin Tang, the early apoptosis of cells was significantly increased, and cell division was significantly blocked in G0/G1 phase, STAT3 protein expression was reduced after 24 h of treatment by Banxia Xiexin Tang,and the high concentration Tang exhibited the best effect. Conclusion: Banxia Xiexin Tang may have the regulation points between G1, S and G2/M phase to affect cell cycle, and its mechanism of promoting apoptosis of tumor cells may be associated with interfering the expression of STAT3 transcription factors.关键词：Banxia Xiexin Tang;esophageal carcinoma;cell cycle;apoptosis;signal transducer and activator of transcription 3
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of chitosan oligosaccharide(CO) on anti-exercise fatigue and exercise-induced immunosuppression. Method: To establish the model of high-intensity endurance training, 55 7-week-old male SPF Wistar rats were randomly divided into 5 groups, with 10 in each group (the rats that did not meet the requirements were removed) : clam group ( C group), exercise group ( E group), exercise + ig low-dose, middle-dose and high-dose chitosan oligosaccharide group ( CO+E-L group, CO+E-M group, CO+E-H group).Gavage was performed by using professional devices once a day.The rats in CO groups were gavaged CO 100, 200, 600 mg ·kg-1 with ig volume of 5 mL ·kg-1 respectively. Rats in other groups were given saline of the same volume.After 42 days of exhaustive swimming training, measuring related indicators. Result: Swimming time in CO+E groups were significantly longer than that in E group ( P< 0.01).There were no difference between C and E groups.Serum superoxide dismutase(SOD) and interleukin-10(IL-10) in E group were significantly lower than C group ( P< 0.01), at the same time, those in CO+E groups were all higher than E groups (P< 0.05, P< 0.01).Serum malondialdehyde(MDA), nuclear factor-kappa B(NF-κB), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and spleen Toll like receptor 4(TLR4) expression ratio in E group were all significantly higher than C group ( P< 0. 01), those in CO+E groups were all lower than that in E group (P< 0.05, P< 0.01). Conclusion: The supplement of CO can effectively relieve the imbalance of internal environment caused by excessive training, avoid the occurrence and development of exercise fatigue and exercise immunosuppression and CO+E-H group have a significant effect.关键词：chitosan oligosaccharide;exercise-induced immunosuppression;exercise-induced immunity;inflammatory factors;Toll like receptor 4
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Shengmai injection combined with glimepiride in the treatment of type 2 diabetes mellitus with Qi and Yin deficiency and its effect on inflammatory factors in serum. Method: Totally 152 patients with type 2 diabetes mellitus in our hospital during February 2013 to February 2015 were randomly divided into observation group (n=77) and control group (n=75). The patients in control group were treated with glimepiride therapy, while the patients in observation group were treated by Shengmai injection on the basis of glimepiride. The clinical efficiency, changes of fasting blood glucose (FBG), postprandial 2 h blood glucose (2 h PBG), and glycosylated hemoglobin (HbA1c), changes in high-sensitivity c-reactive protein (hs-CRP), tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β), as well as side effects between two groups were compared. Result: The total clinical effective rate was 92.21% in observation group, significantly higher than 78.67% in control group, with statistically significant difference between two groups (P<0.05). FBG, 2 h PBG, and HbA1c were (5.87±0.32), (6.52±1.22) mmol · L-1, and (4.42±0.37)% respectively in observation group, significantly lower than (10.63±0.53), (11.24±2.72) mmol · L-1, and(6.21±0.52)% in control group, with statistical difference (P<0.05). After treatment, hs-CRP, TNF-α and IL-1β levels were significantly decreased in both groups (P<0.05), and hs-CRP, TNF-α and IL-1β levels were (2.25±0.73) g · L-1, (1.56±0.47) ng · L-1, and (15.36±3.70) ng · L-1 in observation group, significantly lower than(3.37±1.01) g · L-1, (2.23±0.44) ng · L-1, and (22.94±3.81) ng · L-1 in control group, with statistical difference (P<0.05). The adverse reactions mainly included vomiting, diarrhea, and low blood sugar during treatment in two groups, and it was recovered after symptomatic treatment. There was no statistically significant difference in adverse reaction incidence between the two groups. Conclusion: Shengmai injection combined with glimepiride had an obvious effect in the treatment of type 2 diabetes mellitus with Qi and Yin deficiency, and can reduce the level of inflammatory factors, thus put off the incidence of complications of type 2 diabetes mellitus with Qi and Yin deficiency. It is worthy of clinical popularization.关键词：Shengmai Injection;glimepiride;type 2 diabetes mellitus;glycosylated hemoglobin A1c;interleukin-1β
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发布时间：2024-01-04 - 摘要：Objective: To observe clinical efficacy of Wenxin decoction for unstable angina (UA). Method: A total of 228 patients with unstable angina and diagnosed as coronary heart disease via coronary angiography were enrolled in this study. Coronary artery stenosis occurred in 50%-70% of all the subjects, without any interventional therapy. In respect of traditional Chinese medicine syndrome, this disease was of phlegm and blood stasis stagnation with Yang Qi deficiency. The subjects were randomly divided into treatment group (n=118) and control group (n=110). Patients in treatment group were treated with Wenxin decoction+routine western medicine, while the patients in control group were treated with routine western medicine only. After 60 days of therapy, improvement of angina, consumption of nitroglycerin, changes of high sensitivity C reactive protein (hs-CRP) and low density lipoprotein cholesterol (LDL-C) before and after treatment were compared between two groups. The incidence of major adverse cardiovascular events (MACCE) within 60 days in two groups were compared. Result: There was no statistically significant difference in baseline clinical information between two groups. Total effective rate was 86.4% and 72.7% in treatment group and control group respectively after 60 days of treatment (P<0.01). After treatment, consumption of nitroglycerin was significantly reduced in treatment group, with significant difference as compared with control group (P<0.05). Levels of hs-CRP and LDL-C were also significantly reduced in treatment group, with significant difference as compared with control group (P<0.01). During the follow-up (54.5±8.5 days on average), incidence of MACCE was 12.7% and 24.5% respectively in treatment group and control group (P<0.05), and the non-lethal myocardial infarction rate was 1.7% and 10.0% respectively with statistically significant difference between two groups (P<0.01). There was no significant difference in incidence of revascularization, stroke and cardiac between two groups. Conclusion: Wenxin decoction has better effect in improving the angina symptoms and decreasing hs-CRP, LDL-C in the treatment of unstable angina. It could reduce the incidence of MACCE, especially the incidence of non-lethal myocardial infarction in patients with unstable angina.关键词：Wenxin decoction;unstable angina;high sensitivity C reactive protein;low density lipoprotein cholesterol
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发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy of compound Huangbai fluid for the treatment of diabetic foot ulcer (DFU). Method: The 252 patients with DFU were recruited from the Metabolic Diseases Hospital of Tianjin Medical University between March 2014 and April 2015. They were randomly divided into the experimental group (EG, n = 126) and control group (CG, n=126). The standardized wound treatment was used in both groups, however, the ulcer wounds in the EG were also rinsed and infiltrated with compound Huangbai fluid, while in the CG, normal saline was used for rinsing. After treatment for 8 weeks, the ulcer size, inflammation related indicators, total clinical efficiency and incidence of adverse events were compared with statistical analysis between two groups. Result: The 240 subjects completed this study (n=120 in each group). After treatment for 8 weeks, the EG was superior to CG in term of the reduction of ulcer size,reduction of inflammation related indicators(white blood cell, neutrophil, C-reactive protein, erythrocyte sedimentation rate), and total clinical efficacy (P< 0.05). No significant differences in adverse events were found between two groups. There were no statistical differences in the reduction of ulcer size and inflammation related indicators were observed between ischemic and non-ischemic patients in EG. Conclusion: Compound Huangbai fluid has obvious clinical efficacy on ulcer wound healing and it is applicable to both ischemic and non-ischemic ulcer patients, so it is an effective and safe drug as an adjunctive treatment of DFU.关键词：diabetic foot ulcer;compound Huangbai fluid;adjunctive treatment;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effects of Xuefu Zhuyu Tang combined with Xiaoqinglong Tang in treatment of elderly patients with acute exacerbation stage of chronic pulmonary heart disease. Method: The 140 patients with acute exacerbation of chronic pulmonary heart disease (COPD) were chosen in the period from May 2013 to May 2014 in Qinghai Hospital of Traditional Chinese Medicine, Qinghai Red Cross Hospital, Xining First People's Hospital, and randomly divided into control group (70 patients) and trentment group (70 patients) with random number table method. Patients in control group received routine western medicine treatment, and the patients in trentment group received additional Xuefu Zhuyu Tang combined with Xiaoqinglong Tang on the basis of treatment in control group. The clinical efficacy, trentment symptom scores, levels of pulmonary function index, blood gas analysis index, cardiac function index and blood rheology index before and after treatment of both groups were compared. Result: The clinical efficacy of trentment group was significantly better than that in control group, with statistically significant difference (P<0.05). The trentment symptom scores in trentment group after treatment were significantly better than those in control group and those before treatment, with statistically significant difference (P<0.05). The levels of pulmonary function index and blood gas analysis index after treatment in trentment group were significantly better than those in control group and those before treatment, with statistically significant difference (P<0.05). The levels of cardiac function index and blood rheology index after treatment in treatment group were significantly better than those in control group and those before treatment, with statistically significant difference (P<0.05). Conclusion: Xuefu Zhuyu Tang combined with Xiaoqinglong Tang in treatment of acute exacerbation stage of chronic pulmonary heart disease can efficiently relieve the clinical symptoms and signs, improve heart and lung functions and help to improve blood rheology index.关键词：chronic pulmonary heart disease;acute exacerbation stage;Xuefu Zhuyu Tang;Xiaoqinglong Tang;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of sequential therapies of Shuxuetong injection and Danlou pills on the blood platelet function and risk scores of global registration of acute coronary events (GRACE) in treating acute coronary syndrome (phlegm and blood stasis type). Method: Ninety eligible patients with acute coronary syndrome (ACS) were randomly divided into control group (45 cases) and observation group (45 cases) by random number table. Both groups received percutaneous coronary intervention (PCI) operation. After the operation, patients in control group received treatments of anti-ischemia, lipid-lowering, antiplatelet and anticoagulation, orally taking aspirin enteric-coated tablets, 300 mg for first load dose, and 100 mg/time, 1 time/day for maintenance treatment, clopidogrel bisulfate tablets, 300 mg for first load dose, and 75 mg/time, 1 time/day for maintenance dose. The treatment course was 10 weeks. Based on the treatment of control group, patients in observation group received additional ①Shuxuetong injection for intravenous drip, 6 mL/time, 1time/day, with a treatment course of 2 weeks.② Danlou pills, 5 pills/time, 3 times/day, with a treatment course of 8 weeks. Scores of phlegm and blood stasis were graded before treatment, at Week 2 and week 10 after treatment.GRACE was evaluated at time of admission and discharge. Before treatment and at Week 2 and week 10 after treatment, levels of platelets counts (PLT), mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT) of platelet parameters and platelet aggregation (PAR), platelet-selectin (P-selectin), von willebrand factor (vWF) and GMP-140 platelets surface were detected respectively. Result: At week 2 and week 10 after treatment, scores of phlegm and blood stasis were gradually decreased in both groups (P<0.01), and the scores in observation group were lower than those in control group (P<0.01). At the time of discharge, GRACE score in observation group was lower than that in control group (P<0.01). And at week 2 and week 10 after treatment, levels of PLT, MPV and PDW were decreased in both groups (P<0.05,P<0.01). At week 2 after treatment, levels of MPV and PDW in observation group were decreased more obviously (P<0.05). Levels of PAR, P-selectin, vWF and GMP-140 in observation group were lower than those in control group at week 2 and week 10 after treatment group (P<0.01). Conclusion: sequential therapies of Shuxuetong injection and Danlou pills can inhibit platelet activation in patients with acute coronary syndrome after PCI, reduce thrombosis risk and scores of phlegm and blood stasis and GRACE, so it is helpful to improve prognosis for the patients.关键词：acute coronary syndrome;percutaneous coronary intervention;Shuxuetong Injection;Danlou pills;platelet function;risk score of global registration of acute coronary events
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of on outcomes of precancerous lesions of gastric cancer (PLGC) in patients with chronic atrophic gastritis(CAG) (qi-stagnation syndrome of liver and stomach), as well as on serum trilobites factor 3 (TFF3) and gastric secrete element-17. Method: Eighty-nine PLGC patients were randomly divided into control group (44 cases) and observation group (45 cases) based on the principles of digital meter method to 1 : 1. Patients in control group orally took Weisu granules, 15 g/time, 3 times/day. Patients in observation group orally took Shenzhi Xiaowei decoction, 1 dose/day. Treatment course was 16 weeks for both groups. Histopathological examination was done for gastric mucosa before and after treatment. Gastric cavity pain, fullness,distension, belching and anorexia were scored before and after treatment. TFF3 and gastric secrete element-17 levels were detected before and after treatment. Result: Total effective rate for gastroscope and gastric mucosa clinical pathological effect was 100% in both group, superior to 84.1% in control group (P<0.05). The pathology scores of atrophy degree, intestinal metaplasia (IM) and dysplasia (Dys) in observation group were lower than those in control group(P<0.01). The scores of gastric cavity pain, fullness, distension, belching and anorexia in observation group were lower than those in control group (P<0.01). Serum TFF3 in both group went down obviously after treatment, while the declined degree in observation group was more significant (P<0.01). Serum gastric secrete element-17 level in both group went up obviously after treatment,, while the increase degree in observation group was more significant (P<0.01). Conclusion: Shenzhi Xiaowei decoction can block or reverse the precancerous lesions of gastric cancer (PLGC), and delay the development of CAG to gastric cancer, with significant clinical effect.关键词：chronic atrophic gastritis;precancerous gastritis;Shenzhi Xiaowei decoction;trefoil factor 3;gastric secrete element-17
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effect of Huadu Yuchang decoction in treating ulcerative colitis (UC) of dampness-heat type by oral administration and enema method and investigate its mechanism of action. Method: Ninety-six patients of UC were randomly divided into treatment group and control group, 48 cases in each group. The patients in control group orally took sulfasalazine enteric-coated tablets (4 g · d-1, qid) and triamcinolone tablets (10 mg · d-1,tid). On the basis of the control treatment, the patients in treatment group also received Huadu Yuchang decoction by oral administration (1 dose/d, bid) and enema method (100 mL/time, 3 h/time, once/2 d). The treatment course was 8 weeks for both groups. Traditional Chinese medicine symptoms, pathological scores, colonoscopy scores and Southerland disease activity indexes were compared before and after treatment in 2 groups. The curative effects after treatment were analyzed in 2 groups. Serum levels of interleukin-22 (IL-22) and interleukin-23 (IL-23) were detected before and after treatment in both groups. Result: The total effective rate was 97.92% in treatment group, higher than 81.25% in control group (P< 0.05). Traditional Chinese medicine symptom scores, pathological scores, colonoscopy scores and Southerland disease activity indexes in treatment group were significantly lower than those in control group (P< 0.01). Serum levels of IL-22 and IL-23 in treatment group were significantly lower than those in control group, with statistically significant differences (P< 0.01). Conclusion: Huadu Yuchang decoction treating UC of dampness-heat type by oral administration and enema method could reduce Traditional Chinese medicine symptom scores and Southerland disease activity indexes, improve the pathological and colon conditions, and increase the clinical efficacy. Its action mechanism may be associated with reducing serum levels of IL-22 and IL-23, which is worthy of further discussion.关键词：Huadu Yuchang decoction;enema method;ulcerative colitis;dampness-heat type;interleukin 22;interleukin 23
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发布时间：2024-01-04 - 摘要：Objective: To systematically evalate the safety and efficacy of intergrated traditional Chinese and western medicine in treating infantile rotavirus enteritis. Method: We searched The Cochrane Library, Embase,CNKI,Pubmed,CBM,VIP and Wanfang Data from database inception to June 27,2015.The Randomized Controlled Trials (RCTs) of intergrated traditional Chinese and western medicine in treating infantile rotavirus enteritis in any language were included, and recover into research references. Two researchers extracted data and assessed the literature quality separatedly, and made a Meta analysis using RevMan 5.3 software. Result: 28 RCTs involving 4 227 patients entered the inclusion criteria. The Meta analysis results showed that compared with western medicine, intergrated traditional Chinese and western medicine can significantly improve the clinical tatal effecctive rate[OR=5.10,95%CI(4.07,6.39),P<0.000 01],effectively shorten the defervescence time[MD=-0.30,95%CI(-0.46,-0.13),P=0.000 4],and shorten the time of diarrhoea[MD=-1.42,95%CI(-2.18,-0.66),P=0.000 3],increase the rate of turn rotavirus[OR=5.59,95%CI(3.55,8.78),P<0.000 01],the difference between two groups is statistically significant. ‘Funnel Plot’bias analysis results indicate that there may be a potential publication bias. Conclusion: The current results indicates that intergrated traditional Chinese and western medicine in treating infantile rotavirus enteritis can effectively improve the clinical effective rate,effectively shorten the defervescence time,and shorten the time of diarrhoea,increase the rate of turn rotavirus. However, the quality of methodology and report of small sample of RCTs are low,and the efficacy and safety of intergrated traditional Chinese and western medicine in treating infantile rotavirus enteritis still need more strict, large sample, randomized, double-blind trials to make the conclusion more reliable.关键词：intergrated traditional Chinese-western medicine;rotavirus enteritis;randomized controlled trial;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To systematically review the efficacy of Gegensu injection combined with western medicine for curing Heart failure. Method: Such databases as PubMed, CENTRAL, CNKI, VIP, EMbase, Wanfang and CBM were electronically searched to collect the randomized controlled trials. The document screening and quality evaluation were conducted to extractvalid data for a Meta-analysis. Result: Totally 16 RCTs involving 1 291 cases were included. The results of Meta-analysis showed that compared with the pure western medicine treatment, the combination of Gegensu injection and western medicine were superior in clinical comprehensive effect [OR=3.95, 95%CI(2.84,5.49)], left ventricular ejection fraction [MD=8.43, 95%CI(3.41,13.45)], stroke volume [MD=4.98, 95%CI(3.20,6.75)], cardiac output [MD=0.57, 95%CI(0.19,0.94)], cardiac index [MD=0.77, 95%CI(0.33,1.20)] and reduction of heart rate [MD=9.71, 95%CI(6.52,12.89)], with significant statistical differences (P<0.05). Conclusion: Compared with the pure western medicine treatment, the combination of Gegensu injection and western medicine has a higher clinical efficacy in the treatment of heart failure.关键词：Gegensu injection;heart failure;randomized controlled trial;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the efficacy of Xiyanping combined with Ribavirin in the treatment of children with Pediatric Herpangina. Method: Computer retrieval was conducted in Pubmed, Embase, Cochrane library, CNKI, CBM, Wanfang Data and VIP for randomized clinical trials on Xiyanping combined with Ribavirin in the treatment of children with Pediatric Herpangina. Two researchers independently conducted the strict quality evaluation and data extraction for the included studies and made a Meta-analysis by using RevMan 5.3 software. Result: The 23 eventually included RCTs involved a total of 2 796 cases of children. Meta-analysis showed that Xiyanping combined with Ribavirin in the treatment of children with Pediatric Herpangina were statistically significant in total efficiency, temperature recovery time, herpes regression time compared with the control group (P<0.05). Subgroup analysis showeed Xiyanping combined with Ribavirin in the treatment of children with Pediatric Herpangina were superior to Xiyanping or Ribavirin alone. Conclusion: Xiyanping combined with Ribavirin in the treatment of children with Pediatric Herpangina have a certain efficacy and are superior to Xiyanping or Ribavirin alone. But due to the poor quality of the existing research, more restrictly-designed high-quality, multicenter, randomized, double-blind studies are required for further verification.关键词：Xiyanping;ribavirin;children with pediatric herpangina;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To systemically evaluate the efficacy and safety of traditional Chinese medicine (TCM) tonic class prescriptions in the treatment of amyotrophic lateral sclerosis (ALS). Method: Several foreign language electronic databases such as Pubmed, EMBASE, Cochrane Library and CBM, as well as some Chinese databases such as CNKI, VIP, Wanfang Data Knowledge Service Platform were searched from the date of database creating to December 31, 2014, with amyotrophic lateral sclerosis, motor neuron disease (MND), traditional Chinese medicine, herbs, Chinese medicine, drugs, and Chinese herbal as the key words, to select the randomized control trials on treatment of ALS by Chinese medicine. Meta analysis was performed using Revman Manager 5.2 software. Result: The 7 RCTs involving 411 patients were selected, including 257 cases in treatment group and 154 cases in control group. Meta analysis results showed that the patients in treatment group were treated with TCM tonic class prescriptions (without limiting dose types), better than the control group in terms of symptoms improvement[RR=1.42,95%CI (1.11, 3.38)]. The reduction degree of Norris score in treatment group was less than the control group, so the tonic class prescriptions had better effect than western medicine in controlling ALS, making the disease progress more slowly[MD=2.1, 95%CI(0.65,3.54)];tonic class prescriptions had significant better effect than western medicine in terms of improving impotent disease syndrome[MD=3.29, 95%CI(1.57,5.01)];with statistically significant differences. In 3 of the included studies, drug safety was evaluated from the perspective of gastrointestinal symptoms and liver and kidney functions, and no severe adverse reactions were found. Conclusion: The current limited evidences show that TCM tonic class prescriptions could delay the development of ALS, with better effect than Riluzole, but can not significantly improve symptoms and completely cure ALS. Compared with the western medicine, the TCM is cheaper, and can be preserved easily, so it can reduce the financial burden for patients. However, the low quality of included literature has affected the reliability of this present study. Therefore, further clinical application studies with large size and high quality are still needed.关键词：traditional Chinese medicine tonic class prescriptions;amyotrophic lateral sclerosis;Meta-analysis;randomized controlled trials
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the efficacy and safety of Yadanzi oil grease injection combined with chemotherapy in patients with gastric cancer. Method: Databases such as Pubmed, Embase, Medline, Cochrane Library, CNKI, Wanfang, VIP and CBM were searched for qualified randomized controlled trials (RCTs). Cochrane system evaluation method was used for the methodological quality assessment of included randomized controlled trials (RCTs),and Revman 5.2 software was used for analysis. Result: 9 RCTs involving 594 patients were included. The results of Meta analysis showed that in comparison with chemotherapy alone, the Yadanzi oil grease injection combined with chemotherapy could more significantly increase partial remission rate[OR=1.74, 95%CI(1.23,2.47), P=0.002],overall remission rate[OR=2.45, 95%CI (1.71-3.51), P<0.000 01]and could significantly improve the quality of life for patients (KPS score>10)[OR=2.73, 95%CI (1.25,5.96), P=0.01]. In respect of chemotherapy adverse events, the risks of hand-foot syndrome, nausea and vomiting, diarrhea, leukopenia, thrombocytopenia, and neutropenia in the combinational therapy group were lower than those in chemotherapy alone group(P<0.05). Conclusion: The existing studies showed that compared with chemotherapy alone, Yadanzi oil grease injection combined with chemotherapy can more effectively improve partial remission rate, overall remission rate and quality of life, and the incidence of chemotherapy adverse events was lower in the combinational therapy group. For the quality restriction and possible publication bias of the included studies, more high quality and large sample RCTs are needed to verify the validity.关键词：Radonzi oil grease injection;chemotherapy;gastric cancer;systematic review;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To systematically review the clinical efficacy of Huangqin Tang in patients with ulcerative colitis (UC). Method: Databases such as PubMed, The Cochrane Library, Embase, Web of Knowledge, CBM, Wan fang Data, VIP and CNKI were searched for the randomized controlled trials about Huangqin Tang as the interventions for ulcerative colitis from database creation to January 24th, 2015. Two reviewers independently screened literature, extracted data and evaluated methodological quality according to the inclusion and exclusion criteria. Then Meta-analysis was conducted using RevMan 5.2 software. Result: A total of 5 studies involving 309 patients were included. The results of Meta-analysis showed that, compared with sulfasalazine, Huangqin Tang can significantly improve the cure rate of ulcerative colitis[RR=1.54, 95%CI(1.19,2.01), P=0.001], and significantly reduce the incidence of adverse reactions, but it can not increase the total effective rate of the treatment[RR=1.06, 95%CI(0.97, 1.17), P=0.21]or reduce the clinical symptom scores of diarrhea, bloody purulent stool, tenesmus and stomachache. Conclusion: Huangqin Tang is batter than sulfasalazine in terms of curative rate and adverse reactions, but it still needs more studies for verification.关键词：Huangqin Tang;ulcertive colitis;systematic review;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To summarize the composition principles of prescriptions for infertility from Prescription Dictionary of Traditional Chinese Medicine. Method: Prescriptions used for infertility were selected from the Prescription Dictionary of Traditional Chinese Medicine, and input into the Traditional Chinese Medicine Inheritance Support System (V2.5). Then by using data mining methods, composition principles of the prescriptions were analyzed;and the methods of association rules with apriori algorithm and complex system entropy cluster were used to determine the usage frequency of herbs and association rules between the herbs. Result: The 136 eligible prescriptions were selected and the herbs with high usage frequency in the prescriptions for infertility included ‘Lycii Fructus’, ‘Rehmanniae Radix Preparata’, ‘Angelicae Radix Sinensis’, ‘Semen Cuscutae’, ‘Poria’, and ‘Dioscoreae Rhizoma’. The herb combinations with high usage frequency were ‘Lycii Fructus, Cuscutae Semen’, ‘Lycii Fructus, Rehmanniae Radix Preparata’, ‘Angelicae Radix Sinensis, Rehmanniae Radix Preparata’, and ‘Schisandrae Chinensis Fructus, Cuscutae Semen’. The association rules with high confidence coefficient included ‘Dioscoreae Rhizoma, Rehmanniae Radix Preparata-> Corni Fructus’, ‘Dioscoreae Rhizoma, Cuscutae Semen->Lycii Fructus’, and ‘Achyranthis Radix Bidentatae-> Lycii Fructus’. Four new prescriptions were obtained based on the composition principles and rules, including ‘Rehmanniae Radix Preparata, Ophiopogonis Radix, Asparagi Radix, Polygalae Radix, Morindae Officinalis Radix, Eucommiae Cortex’, ‘Rehmanniae Radix Preparata, Ophiopogonis Radix,Ginseng Radix et Rhizoma, Halitum, Zanthoxyli Pericarpium, Cera Flava’, ‘Allii Tuberosi Semen, Os Draconis, Rubi Fructus, Cuscutae Semen, Corni Fructus, Schisandrae Chinensis Fructus,Dioscoreae Rhizoma’, ‘Polygalae Radix, Morindae Officinalis Radix, Cistanches Herba, Ophiopogonis Radix, Phellodendri Chinensis Cortex, Rehmanniae Radix, Ophiopogonis Radix’. Conclusion: The common used herbs for infertility are sweet and warm-natured and go into the channels of kidney, liver and heart, and the Chinese medicine applied in the prescriptions for infertility mostly have the tonifying effects, invigorating Yin, Yang and Qi, and most of the prescriptions could tonify liver and kidney simultaneously. The new prescriptions for infertility are mainly of tonifying effects, with the characteristics of ‘invigorating the kidney and strengthening Yang’ and ‘restoring normal coordination between heart and kidney’. This pattern will provide new prescription ideas and thoughts for clinical practice.关键词：infertility;composition principles;Traditional Chinese Medicine Inheritance Support System;data mining
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发布时间：2024-01-04 - 摘要：Objective: To evaluate clinical efficacy of Liuwei Dihuang prescription in treatment of menopausal syndrome. Method: Searched WHOICTRP,The Cochrane Library,PubMed,VIP,Wanfang Data,CBM and CNKI on computer,randomized controlled trials of Liuwei Dihuang prescription in treatment of menopausal syndrome were collected,retrieval from inception to January 30,2015.Two reviewers screened literature according to inclusion and exclusion criteria,extracted data,and assessed methodological quality of these included studies.Meta-analysis was performed by RevMan 5.2 software. Result: A total of 18 randomized controlled trials involving 1 981 patients were included.Meta-analysis showed thatin treatment of climacteric syndrome either alone or in combination with other Chinese patent medicinesof Liuwei Dihuang prescription,the total clinical efficacy was superior to western medicines or other Chinese patent medicines.In the recovery rate,Liuwei Dihuang prescription was better than taking other Chinese patent medicines in treatment of climacteric syndrome,but its medication alone or combined with other Chinese patent medicines did not show better than western medicines.In terms of reducing follicle-stimulating hormone (FSH),Liuwei Dihuang prescription alone or combined with other drugs were better than western medicines or other Chinese patent medicines,single use of Liuwei Dihuang prescription or combination therapy in reducing luteinizing hormone (LH) had no better than western medicines.Single use of Liuwei Dihuang prescription or combination therapy in elevation ofestradiol (E2) single were better than western medicines or other Chinese patent medicines,in term of reducing Kupperman score,Liuwei Dihuang prescription had no better than other Chinese patent medicines. Conclusion: Single use of Liuwei Dihuang prescription or combined with other Chinese patent medicines in treatment of climacteric syndrome is better than other Chinese patent medicines and western medicines without obvious adverse reactions reported in literature,because quality of literature is not high,it still needs to support a large sample randomized double-blind test theory.关键词：Liuwei Dihuang prescription;climacteric syndrome;randomized controlled trials;systematic review;database
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发布时间：2024-01-04 - 摘要：Cardiovascular disease is the first cause of death and disability among three major diseases, seriously impairing human health and life quality. Heart Qi deficiency syndrome is the most common traditional Chinese medicine syndrome of cardiovascular disease, so the study of animal models of heart Qi deficiency has important guiding effect and great application value for the prevention and treatment of cardiovascular disease.The experimental study is based on animal models, so the methods to establish animal models shall be in line with the actual development process of disease, and the evaluation indicators of animal models shall also correspond to the clinical indicators to the greatest extent. This article introduces the research status of the establishment and evaluation of animal models of heart Qi deficiency, proposes the problems existing in the establishment of heart Qi deficiency-animal models, and provides reference for the establishment and study of this animal model.关键词：heart Qi deficiency;animal model;evaluation criteria
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