最新刊期
卷 22 ， 期 5 ， 2016
- 摘要：Objective: To discuss mechanism of stir fried with salt into the kidney-the kidney controlling water metabolism by analyzing effects of being processed of Alpiniae Oxyphyllae Fructus in Suoquan Wan on renal function of adenine-induced kidney-yang deficiency rats with much urine and expression of aquaporin-2(AQP-2). Method: Effects of being processed of Alpiniae Oxyphyllae Fructus in Suoquan Wan on urine volume,viscera indexes,creatinine (Cr),urea nitrogen (BUN) and β2-microglobulin (β2-MG) in blood,AQP-2 mRNA and protein expression of adenine-induced kidney-yang deficiency rats were investigated. Result: Compared with the model group,each treatment group could reduce urine of rats,change of viscera indexes could be obviously inhibit,contents of Cr,BUN and β2-MG in blood significantly reduced,expression of AQP-2 mRNA and protein increased.After being processed with salt of Alpiniae Oxyphyllae Fructus in Suoquan Wan,effects strengthened obviously in comparison with the raw group. Conclusion: Alpiniae Oxyphyllae Fructus before and after being processed in Suoquan Wan have improving effects on renal function and protein expression of AQP-2 of adenine-induced kidney-yang deficiency rats,but effects strengthen obviously after salt processing.关键词：Suoquan Wan;Alpiniae Oxyphyllae Fructus;salt processing;renal function;AQP-2
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发布时间：2024-01-04 - 摘要：Objective: To compare contents of alkaloids between Coptidis Rhizoma,bile processed products of Coptidis Rhizoma and their water decoctions,dissolution of alkaloids in Coptidis Rhizoma was determined after being processed with pig's bile.Aim of this study was to explore processing mechanism of cold property enhancement of bile processed products. Method: Total alkaloids in Coptidis Rhizoma,bile processed products and their water decoctions were determined by UV.Contents of berberine,jatrorrhizine and palmatine in Coptidis Rhizoma,bile processed products and their water decoctions were determined by HPLC,mobile phase was acetonitrile-phosphate triethylamine solution by gradient elution at room temperature,detection wavelength was set at 345 nm. Result: Transfer rates of total alkaloids from water decoctions of Coptidis Rhizoma and its bile processed products were 71.17% and 78.50%;transfer rates of jatrorrhizine,palmatine and berberine in water decoction of Coptidis Rhizoma were 73.93%,51.59% and 56.16%;transfer rates of jatrorrhizine,palmatine and berberine in water decoction of bile processed products of Coptidis Rhizoma were 80.42%,64.21% and 64.79%,respectively. Conclusion: Being processed with pig's bile can raise transfer rate of total alkaloids in water decoction of Coptidis Rhizoma,and then to achieve purpose of cold property enhancement of bile processed products of Coptidis Rhizoma.关键词：Coptidis Rhizoma;bile processed products of Coptidis Rhizoma;alkaloids;transfer rate;jatrorrhizine;palmatine;berberine
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for evaluating quality correlation of raw herbs-decoction pieces-extract-dispensing granules of Chaenomelis Fructus from Xuancheng city,and to provide a basis for quality control of dispensing granules in whole process of production. Method: RP-HPLC was employed to determine contents of oleanolic acid and ursolic acid in samples with mobile phase of methanol-water-acetic acid-triethylamine (265 : 35 : 0.1 : 0.05),detection wavelength at 210 nm and flow rate of 0.6 mL · min-1,their characteristic spectrum were analyzed. Result: Contents of oleanolic acid and ursolic acid in five batches of raw herbs of Chaenomelis Fructus met standard of the 2015 edition of Chinese Pharmacopoeia,which in its decoction pieces,extract and dispensing granules were relatively stable.There was a good correlation among characteristic spectrum of raw herbs,decoction pieces,extract and dispensing granules,there were six common peaks in all characteristic spectrum of them. Conclusion: This method can be used to characterize consistency of active ingredients in raw herbs,decoction pieces,extract and dispensing granules,and to control quality of Xuanmugua dispensing granules in whole process of production.关键词：Chaenomelis Fructus from Xuancheng city;dispensing granules;oleanolic acid;ursolic acid;characteristic spectrum;quality correlation
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of an immune active protein (AmPR10-16) with a molecular weight of 16.0 kDa from Astragali Radix. Method: Extraction temperature was investigated by circular dichroism of water-soluble protein involving in AmPR10-16 with secondary structure from Astragali Radix.Extraction technology was optimized by single factor test and orthogonal test with gray value of AmPR10-16 as index which was determined by gel graphical analysis software. In this study,effects of temperature,solid-liquid ratio,time,solvent,granularity and times on gray value were investigated,for which determination of water-soluble protein was determined as an evidence by BCA method. Result: Optimum extraction process of AmPR10-16 was as follows:with Tris-HCl with pH 8.0 as solvent,solid-liquid ratio of 1 : 16,extraction time of 60 min with temperature at 40 ℃,medicinal powder over the 4th sieve,stirring once every 10 min.Yield of AmPR10-16 was 0.063 g · g-1. Conclusion: This optimal extraction technology can accurately reflect relative amounts of AmPR10-16 maximum extraction rate,it provides a stable,reasonable and feasible extraction process for furter study of AmPR10-16.关键词：Astragali Radix;protein;secondary structure of protein;SDS-PAGE;BCA method
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology and alcohol precipitation process of water-soluble polysaccharides form Meretrix meretrix,and provide experimental evidence for health products development of this effective part. Method: Content of water-soluble polysaccharides was determined by anthrone-sulfuric acid method at 625 nm.Water-soluble polysaccharides was islolated by water extracting alcohol precipitating method,taking yield,transfer rate and content of polysaccharides as indexes,process conditions were optimized by orthogonal test and single factor test. Result: Optimum process conditions for water-soluble polysaccharides were as followings:extracted thrice with 3 times the amount of water at 80 ℃,40 min for each time,extract concentrated to half of raw material,added ethanol to volume fraction of 80%,alcohol precipitation for 12 hours,dried at 60 ℃.Under these conditions,average yield of water-soluble polysaccharides was 7.71% and its average content was 42.53%. Conclusion: This optimized technology is stable and feasible with high extracting rate and transfer rate of water-soluble polysaccharides in M.meretrix,it can balance purity and extracting rate of this effective part.关键词：Meretrix meretrix;total polysaccharides;anthrone-sulfuric acid method;transfer rate
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发布时间：2024-01-04 - 摘要：Objective: To screen optimal shaping and matching excipients of Tiaowei Chengqi granules for solving moldability,dissolvability, hygroscopicity and other issues. Method: With appearance,particle size,moisture content,dissolvability and hygroscopicity of granules as indexes,single factor tests were adopted to screen kinds,ratio and amounts of excipients,then quality verification,critical relative humidity and angle of repose were investigated. Result: Optimum molding materials was mixture of lactose and mannitol,ratio of extract-lactose-mannitol (1 : 3 : 1) for granulating,these prepared granules had uniform particle size,good properties and were not easy to moisture absorption.Critical relative humidity was 69% and angle of repose was less than 40°. Conclusion: These optimized excipients can significantly improve stability of Tiaowei Chengqi granules,and this molding process is scientific and rational.关键词：Tiaowei Chengqi granules;molding process;excipients;Natrii Sulfas;angle of repose;critical relative humidity
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发布时间：2024-01-04 - 摘要：Objective: To reveal the change regularity in contents of total polysaccharides and alkaloids and select superior families from seedlings of different F1 generations hybridized from superior parents of Dendrobii Officinalis Caulis. Method: The 26 superior parents from 4 provenances were selected to achieve 26 mating combinations. Contents of total polysaccharides and total alkaloids were determined by methods of phenol-sulphate colorimetry and acid-dye colorimetry respectively. The superior families were selected using correlation analysis and cluster analysis combined with the previous agronomic traits. Result: There were extremely significant differences in contents of polysaccharides and alkaloids among different mating combinations (P<0.01). There was significant difference in content of polysaccharides among half-sib families (P<0.05), but no significant difference was found in content of alkaloids among different half-sib families. Meanwhile, the variations of polysaccharides and alkaloids contents in full-sib families were significantly lower than those in half-sib families. Moreover, the number of leaves in seedling stage of Dendrobii Officinalis Caulis and the polysaccharide content;length-width ratio of leaves, root-shoot ratio and the alkaloids content were positively correlated, respectively. The 26 mating combinations were divided into 5 groups. Plants from the second group grew much better than the others. Conclusion: The selection of parents should be emphasized when selecting superior families from different F1 generations. There were higher genetic gains and superior uniformity in full-sib families than those in half-sib families. Combined with the previous agronomic traits, 6 superior families (66×9, 68×2, 91×69, 66×65, 69×91, 66×17) were selected, presenting superior characteristics of seedlings including high and thick stem with less branch, large and long leaves, strong deep roots and high content of total polysaccharide. The work has laid foundations for the later selection.关键词：Dendrobii Officinalis Caulis;F1 generations;seedling stage;superior families
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发布时间：2024-01-04 - 摘要：Objective: To investigate quality of Curcumae Longae Rhizoma from different areas by analyzing correlation relationship of demethoxycurcumin,bisdemethoxycurcumin,curcumin and volatile oil in this herb. Method: Samples of Curcumae Longae Rhizomafrom different areas were collected,contents of curcuminoids were quantified by HPLC-UV whereas volatile oil was determinate by this method in the 2010 edition of Chinese Pharmacopoeia.Determination were analyzed by cluster and person correlation analysis with SPSS 20.0 software. Result: Effective ingredientsin Curcumae Longae Rhizomafrom different areas had a remarkable difference,when a maximum value of curcuminoids was about 4.9%,a minimum value was about 0.2%.Content of volatile oil in Curcumae Longae Rhizoma between the maximum and minimum values reached 4.2 times.Significant positive correlation had been showed between demethoxycurcumin,bisdemethoxycurcumin,curcumin and volatile oil. Conclusion: Altitude difference has effecton contents of curcuminoids in Curcumae Longae Rhizoma.Farming location of Curcumae Longae Rhizoma need to carry on reasonable planning to improve its quality and to promote sustainable development of industry.关键词：Curcumae Longae Rhizoma;curcuminoids;volatile oil;curcumin;demethoxycurcumin;bisdemethoxycurcumin
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发布时间：2024-01-04 - 摘要：Objective: To compare contents of volatile oil,α-asarone and β-asarone in rhizomes and leaves of fresh and dry products of Acori Tatarinowii Rhizoma,in order to provide experimental basis for reasonable appilication of this herb. Method: Volatile oil in Acori Tatarinowii Rhizoma was extracted by steam distillation,α-asarone and β-asarone in rhizomes and leaves were extracted by ultrasonic extraction method.HPLC was employed to determine contents of α-asarone and β-asarone with mobile phase of water-methanol (65 : 35) and detection wavelength at 257 nm. Result: The content of volatile oil in fresh products was markedly higher than that in dry products,this index in rhizomes was higher than that in leaves.Contents of α-asarone and β-asarone in fresh products was higher than that in dry products,the content of α-asarone in leaves was higher than that in rhizomes,the content of β-asarone in rhizomes was higher than that in leaves. Conclusion: Difference of active ingredients between dry and fresh products is small,both of them can be used in medicine.Contents of active ingredients in leaves can not be ignored,allowing for a large proportion of leaves in the whole herb,leaves of Acori Tatarinowii Rhizoma could be used in medicine.关键词：Acori Tatarinowii Rhizoma;volatile oil;β-asarone;α-asarone;fresh herbs
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发布时间：2024-01-04 - 摘要：Objective: To optimize the screening, sterilization and culture medium for explants of Trillium tschonoskii, reduce the pollution rate and browning rate, and establish an aseptic tissue culture system of T. tschonoskii. Method: Most suitable explants were selected from ovary, leaves, stems, underground rhizomes and root tips of T. tschonoskii, and the effect of a variety of drug liquid alternating sterilization and addition of antibiotics in medium were studied on the contamination rate. VitC(VC), polyvinyl pyrrolidone(PVP) and activated carbon were added to the medium to study their effect on reducing the browning rate. Result: The underground rhizome of T. tschonoskii was the most optimum explants. It could be alternately sterilized with ultraviolet radiation for 30 min, 800 times of carbendazim soaking for 30 min, and processed with 0.2%HgCl2 for 20 min. The medium supplemented with 100 mg · L-1 ampicillin and 1.0 g · L-1activated carbon could reduce the contamination rate and browning rate of the rhizome tissue culture. Conclusion: The rhizome sterile culture system of T. tschonoskii has been established with low contamination rate and browning rate. It can provide reference for tissue culture of other rhizome-plants.关键词：Trillium tschonoskii;explants;pollution;browning
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents and their anti-angiogenic activities of the fruits of Evodia rutaecarpa. Method: Compounds were isolated and purified by column chromatography using silica gel, Sephadex LH-20, ODS and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties and spectral data. The anti-angiogenic activities of compounds were evaluated using a zebrafish model. Result: Twenty compounds were isolated and identified from the fruits of E. rutaecarpa, including one degraded limonoids calodendrolide(1),6 indole alkaloids, i.e. rutaecarpine(2), evodiamine(3), goshuyuamide-Ι(4), N-formyldihydrorutaecarpine(5), and 7β-hydroxyrutaecarpine(6),11 quinolone alkaloids, i.e. 2-hydroxy-4-methoxy-3-(3'-methyl-2'-butenyl)-quinoline(7), 1-methyl-2-nonyl-4(1H)-quinolone(8), 1-methyl-2-decyl-4(1H)-quinolone(9), 1-methyl-2-undecyl-4(1H)-quinolone(10), dihydroevocarpine(11), 1-methyl-2-pentadecenyl-4(1H)-quinolone(12), 1-methyl-2-[(Z)-6-undecyl]-4(1H)-quinolone(13), evocarpine(14), 1-methyl-2-[(Z)-4-tridecyl]-4(1H)-quinolone(15), mixture of 1-methyl-2-[(Z)-10-pentadecenyl]-4(1H)-quinolone and 1-methyl-2-[(Z)-6-pentadecenyl]-4(1H)-quinolone(16), 1-methyl-2-[(6Z, 9Z)-6, 9-pentadecenyl]-4(1H)-quinolone(17), 2 amides, i.e. N-methylanthranylamide(18), acetamide(19), and one sterol, i.e.β-sitosterol(20). Compounds 1 (20 mg · L-1), 2 (0.5 mg · L-1), 3 (5, 10 μg · L-1), 4 (10 mg · L-1), 5 (50 mg · L-1), 10 (20 mg · L-1), 11 (50 mg · L-1), 16 (20 mg · L-1), and 18 (50 mg · L-1) showed anti-angiogenic effects on internode blood vessels of Zebrafish models. Conclusion: Compounds 1-18 were characteristic constituents of Euodiae Fructus, and compounds 1 and 7 were isolated from this plant for the first time. Compounds 1-5, 10, 11, 16, and 18 showed anti-angiogenic effects on internode blood vessels of Zebrafish models.关键词：Euodiae Fructus;alkaloids;degraded limonoids;Zebrafish model;anti-angiogenic activities
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发布时间：2024-01-04 - 摘要：Objective: To establish a rapid inductively coupled plasma-mass spectroscopy (ICP-MS) method for determing the content of 25 inorganic elements such as Be, Na, Mg, Al, K, Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As and Se in Tougucao. Method: Samples were treated by microwave digestion, and 25 inorganic elements in 18 batches of samples were determined with ICP-MS method. Principal component analysis and cluster analysis were done by Mass Profiler Professional(MPP). Result: The correlation coefficient values of regression equation were over 0.999 for various elements. The method exhibited a recovery range of 91.1%-106.3%. There was great difference in content of inorganic elements in Tougucao from different origins and areas, and the elements presented regular wave type distribution with the atomic number order. The top five elements with high average content were as follows from high to low:K(19 970.00 μg · g-1), Ca(15 709.00 μg · g-1), Mg(3 531.50 μg · g-1), Al(1 755.50 μg · g-1), and Fe(1 021.00 μg · g-1). Principal component analysis results showed that Be, Na, Al, Cr, Mn, Fe, Co, Zn, As, Ba, Tl and U were the characteristic inorganic elements of Tougucao. In clustering analysis, 18 batches of samples were clustered into 2 categories, Vicia cracca clustered into one category;and the other five sources of Vicia clustered into another category, indicating that V. cracca had a far genetic relationship with other five types of Vicia. Conclusion: This determination method provides a high sensitive and accurate way to simultaneously determine multiple inorganic elements and provides experimental basis for evaluation of effectiveness and safety as well as quality standard establishment of Tougucao.关键词：Tougucao;ICP-MS;inorganic elements;full quantitative analysis;principal component analysis;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for simultaneous determination of four constituents (paeoniflorin,ferulic acid,verbascoside and spinosin) in Yijing granules. Method: Column was Kromasil C18 (4.6 mm×250 mm,5 μm),mobile phase was 0.1% acetic acid-acetonitrile (84 : 16),flow rate was 1.0 mL · min-1 and detection wavelengths were set at 230 nm for paeoniflorin and 330 nm for ferulic acid,verbascoside and spinosin. Result: Linear ranges of paeoniflorin,ferulic acid,verbascoside and spinosin were 0.360 2-1.801 2,0.170 6-0.852 9,0.055 98-0.279 9,0.055 44-0.277 2 μg,respectively.Average recoveries were 101.14%,98.97%,98.81% and 97.92% with RSD of 2.0%,1.9%,2.7% and 1.8%,respectively. Conclusion: This developed HPLC method is convenient and reliable,which could be applied to determine contents of four constituents in Yijing granules.关键词：Yijing granules;paeoniflorin;ferulic acid;verbascoside;spinosin
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发布时间：2024-01-04 - 摘要：Objective: To determine oil-water partition coefficient and equilibrium solubility of colchicine in water system with different pH. Method: The content of colchicine was determined by HPLC,apparent octanol-water partition coefficient and equilibrium solubility of colchicine was measured by shake-flask method.Chromatographic conditions were C18 column,methanol-water (40 : 60) as mobile phase and detection wavelength at 254 nm. Result: In solutions of water,pH 1.2,2.0,4.0,6.8 and 7.4 buffer,equilibrium solubilities of colchicine were 12.99,14.77,15.32,23.99,12.28, 12.36 g · L-1,partition coefficients were 4.05,6.62,6.31,5.34,8.88 and 7.13,respectively. Conclusion: There is no correlation between colchicine oil-water partition coefficient,solubility and pH.Colchicine has characteristics of high solubility and low permeability.There may be some factors that restrict colchicine's permeability except oil-water partition coefficient.关键词：colchicine;oil-water partition coefficient;equilibrium solubility;P-glycoprotein
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发布时间：2024-01-04 - 摘要：Objective: To study the regularity of extraction time, pH of the phenolic acids in Salviae Miltiorrhizae Radix et Rhizoma. Method: Respectively investigates the Salviae Miltiorrhizae Radix et Rhizoma at different heating time, pH, and the reaction time of phenolic acids peak area change rule by High-performance liquid chromatography. Result: The content of salvianolic acid B was increasing and reached the maximum at 30 min,but it decreased from 1 to 6 h. Tanshinol,protocatechuic aldehyde, salvianolic acid A is the final product. Salvianolic acid A could be converted into Salvianolic acid C and iso-salvianolic acid C by alkaline hydolysis. Conclusion: Reveals transformation of phenolic acids under heating, pH conditions,providing theoretical support in future Chinese medicine production and clinical applications of Salviae Miltiorrhizae Radix et Rhizoma.关键词：Salviae Miltiorrhizae Radix et Rhizoma;tanshinol;salvianolic acid B;salvianolic acid A;conversion
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发布时间：2024-01-04 - 摘要：Objective: To establish the fingerprints of Shuanglianfang based on full-time multi-wavelength fusion method, and provide basis for the quality control of Shuanglianfang. Method: High performance liquid chromatography (HPLC) method was used on Welch Ultimate LP-C18 column (4.6 mm×250 mm,5 μm), at the mobile phase of 0.1% methanoic acid solution (A) and ethanol (B) for gradient elution (0-20 min, 25%-60%A; 20-40 min, 60%-65%A;40-70 min, 65%-45%A). The flow rate was maintained at 1.0 mL · min-1and the temperature was maintained at 30 ℃. Detector wavelengths were 254, 300, 320, 365 nm. Result: With the chromatographic peak of luteolin as the reference peak, 18 chromatographic peaks in total were identified, and the full-time multi-wavelength fusion fingerprints of Shuanglianfang were established. Based on the HPLC fingerprints, relative quantification of other components in Shuanglianfang was conducted, and the content of other components in Shuanglianfang shall not be less than 0.39, 0.24, 0.46, 0.45, 0.73, 0.28, 0.16, 0.41, 0.51, 2.37, 0.77, 0.22, 0.60, 0.36, 0.62, 0.80, 0.52, and 0.96 relative to luteolin. Conclusion: This method is simple, accurate, scientific, and suitable for the quality evaluation of Shuanglianfang.关键词：Shuanglianfang;fingerprints;full time multi-wavelength fusion;luteolin
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发布时间：2024-01-04 - 摘要：Objective: To set up the multi-wavelength fusion UPLC fingerprint of 10 batches of Chuanxiong Rhizoma from different regions, initially identify common peaks, and compare the Chuanxiong Rhizoma from different regions through fingerprints, and provide the basis for its quality control and medicinal material identification. Method: UPLC was used to set up the multi-wavelength fingerprints of Chuanxiong Rhizoma from different regions. SPSS 19.0 data statistics software was used to analyze the differences of Chuanxiong Rhizoma in different regions, and full time multiple wave length fusion technology was used for multiple wavelength fusion of dif date. Q-TOF method was used to identify the common peaks in fingerprints. Result: Fingerprints of multi-wavelength fusion of Chuanxiong Rhizoma were established;20 common peaks were determined and 8 peaks of them were identified. By clustering analysis and similarity evaluation, it was found that Yunnan Chuanxiong Rhizoma had certain difference with other regions. Conclusion: The fingerprints were reproducible and can be able to distinguish the Chuanxiong Rhizoma from different regions. This method can be applied for quality control of Chuanxiong Rhizoma.关键词：Chuanxiong Rhizoma;UPLC fingerprint;multi-wavelength fusion;Q-TOF-MS
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发布时间：2024-01-04 - 摘要：Objective: Through the content determination of chlorogenic acid, mignonette glycosides, total phenolic acid, and total flavonoid in flos lonicerae from 14 different regions, to find out the correlation between the four components, and provide a reference for reasonable assessment of the quality of Loniceroe Japonicae Flos. Method: HPLC and UV method were used to determine the content of chlorogenic acid, mignonette glycosides, total phenolic acid and total flavonoid in Loniceroe Japonicae Flos, and correlation analysis method was used to measure their relationships. Result: With the content of four components as the comprehensive indicators, Loniceroe Japonicae Flos from Linyi in Shandong had the highest content in chlorogenic acid, mignonette glycosides, total phenolic acid, and total flavonoid. Through correlation evaluation, chlorogenic acid and total phenolic acid in Loniceroe Japonicae Flos were highly correlated;mignonette glycosides and total flavonoid were moderately correlated;chlorogenic acid and mignonette glycosides were moderately co-related;total phenolic acid and flavonoids were moderately correlated. Conclusion: The evaluation of index components and related analysis of Loniceroe Japonicae Flos from 14 different origins provide experimental data for quality evaluation and rational utilization of Loniceroe Japonicae Flos.关键词：Loniceroe Japonicae Flos;chlorogenic acid;mignonette glycosides;total phenolic acids;total flavonoids
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of the inorganic elements in Poria cocos and Polyporus from 5 different areas by inductively coupled plasma mass spectroscopy (ICP-MS), analyze the regional difference of inorganic element contents and correlation between inorganic elements of the drugs, and investigate the relationship between the diuretic activity and elements content. Method: Full quantitative analysis was done for the inorganic elements in Poria and Polyporus by microwave digestion-ICP/MS. In the process of data analysis, MPP (12.60) software was employed for principal component analysis (PCA) and clustering analysis (CA), and SPSS (17.0)software was employed for the analysis of correlation between elements content. Result: The content of elements in the samples showed similar regularity with the increase of atomic number. The content of the inorganic elements in Poria and Polyporus from main producing areas was higher than that from other areas. The PCA and CA results showed that there was significant difference in inorganic elements content between these two herbs. The Poria from Yunnan, Guangxi and Guizhou had closer results in content of inorganic elements, and Hunan and Anhui had similar results. The Polypor from Shanxi, Shaanxi and Anhui had closer results in content of inorganic elements, and Liaoning and Jilin had similar results. Correlation results showed that 55 pairs of elements had correlation (P<0.05), and 121 paris of elements had significant correlation (P<0.01). Macro element Mg had significant correlation with Na, K. Most trace elements had significant correlation. There was also significant correlation between macro elements and trace elements. Conclusion: The method provided a suitable way to the simultaneous determination of multiple elements in Poria and Polypor. The genus genetic relationship and the quality of the herbs were closely related to areas;the absorption of inorganic elements may be selective, and its absorption ratio may be associated with the pharmacological effects and strength of the herbs.
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发布时间：2024-01-04 - 摘要：Objective: To establish a quantitative analysis of multi-components with a single-marker (QAMS) method for the determination of eight components in Qianlielong Bitong tablets (polydatin, naringin, hesperidin, neohesperidin, epimedin A, epimedin B, epimedin C, icariin) and make more comprehensive assessment of the quality of the drug under 270, 283 and 306 nm wavelengths of chromatogram using full-time multi-wavelength fusion technology. Method: With Qianlielong Bitong tablets as the research object and neohesperidin as the internal reference substances, the relative correlation factors (RCF) of polydatin, naringin, hesperidin, epimedin A, epimedin B, epimedin C, icariin to neohesperidin were calculated and established.The content of neohesperidin in the samples of Qianlielong Bitong tablets was determined by using the external standard method, and the contents of the other seven ingredients were calculated by their RCFs. The QAMS results were then compared with the external standard method results. Result: The values of 8 active constituents in four batches of Qianlielong Bitong tablets calculated by QAMS method were basically the same with the values calculated by external standard method, thus verify the feasibility and accuracy of QAMS method. Conclusion: The QAMS method is feasible and credible, and could be used to determine the multiple components in Qianlielong Bitong tablets.关键词：quantitative analysis of multi-components by single marker;Qianlielong Bitong tablets;full-time multi-wavelength fusion;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of the six flavonoid active components (hyperoside, isoquercitrin, astragalin, quercetin, kaempferol and isorhamnetin) in Semen Cuscutae by UPLC. Method: A quantitative method was developed for simultaneous determination of the six flavonoid active components with Semen Cuscutae from Inner Mongolia as a representative. Agilent Eclipse Plus C18 column (2.1 mm×50 mm, 1.8 μm) was used with the mobile phase of acetonitrile-0.1% formic acid solution for gradient elution, at a flow rate of 0.21 mL · min-1. The column temperature was kept at 30 ℃ and the wavelength was set at 360 nm. Result: Hyperoside, isoquercitrin, astragalin, quercetin, kaempferol and isorhamnetin showed good linearity in range of 0.046 8-0.748 8 μg(r=0.999 9), 0.024 2-0.387 2 μg(r=0.999 9), 0.088 2-1.411 2 μg(r=0.999 9), 0.002 3-0.037 0 μg(r=0.999 7), 0.028 8-0.460 8 μg(r=0.999 6) and 0.002 3-0.037 0 μg (r=0.999 4) respectively. The detection limit of six flavonoids was 0.292 5, 0.302 5, 0.367 5, 0.289 0, 0.360 0,0.286 0 mg · L-1, and their limit of quantification was 0.877 5, 1.210 0, 1.470 0, 1.012 0, 1.260 0, 1.215 5 mg · L-1 respectively. Their average recovery rate was 99.95%, 99.78%, 99.53%, 99.38%, 100.03% and 99.35% with RSD of 1.0%, 1.2%, 1.0%, 1.4%, 1.1% and 0.5% respectively. There was great difference in contents of the six flavonoid active components between 12 kinds of Semen Cuscutae from different origins. Conclusion: This method is specific, reproducible,rapid and controllable, and provides basis for scientific evaluation and quality control of Semen Cuscutae.关键词：Semen Cuscutae;different origins;flavonoids;UPLC
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of volatile index component limonene in Qizhi Weitong granules, and improve the quality control standard of volatile components in Qizhi Weitong granules compound. Method: In this study, high performance liquid chromatography (HPLC) was used to investigate the optimum pretreatment process of limonene in sugarless Qizhi Weitong granules, and a methodological study was done for this method.At the same time, content of limonene in 10 batches of Qizhi Weitong granules was detected and their content limits were determined, and to establish a rapid identification by infrared spectroscopy easy way limonene content. Result: The obtained optimum pretreatment method for limonene was as follows: take sugarless Qizhi Weitong granules 2.5 g, add methanol 50 mL, and with ultrasonic treatment (power 250 W, frequency 40 kHz) for 30 min. Methodological study identified a linear range of limonene between 0.17-1.68 μg (r=0.999 8), and the average recovery rate was 99.77%, and the content limit of limonene in Qizhi Weitong granules was determined as not less than 1.00 mg. Gray correlation analysis were obtained 3 and limonene content related peaks. Conclusion: The method is simple, scientific and reasonable, and can be used as a method for the determination of limonene content in Qizhi Weitong granules. The present study has great significance for quality evaluation of Qizhi Weitong granules, and provides great supplementary for the full and comprehensive evaluation of traditional Chinese medicine prescriptions. The study combined data analysis of gray theory, construction of the coarse particle inspection Qizhiweitong limonene content of infrared spectroscopy, for the rapid identification of stomach Qi stagnation particles limonene provides an effective, simple and intuitive way, for the full, comprehensive evaluation of traditional Chinese medicine prescription preparations Qizhiweitong the quality of the particles provide a strong complement.关键词：Qizhi Weitong granules;limonene;quality control;infrared spectroscopy
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发布时间：2024-01-04 - 摘要：Objective: To Detemine human and rat plasma protein binding rate of allicin,and provide a reference for research of allicin into medicinal properties. Method: With ethylparaben as an alternative reference, C18 column was used,mobile phase was methanol-1% formic acid for gradient elution in a flow rate of 1.0 mL · min-1 and detection wavelength was 254 nm. Result: With different concentration of allicin including 75,150,300 mg · L-1, protein binding rates of allicin in rat and human plasma were 23.2%,19.8%,28.1% and 15.5%,25.8%,40.0%,respectively. Conclusion: Allicin has low level in binding to plasma protein of human and rat.HPLC with ethylparaben as alternative reference can be used to determine plasma protein binding rate of allicin.关键词：allicin;plasma protein binding rate;alternative reference;ethylparaben;butylparaben;Ultrafiltration
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Tongxinluo submicron powder combined with Jinlida granules on protein kinase B(Akt)/mammalian target of rapamycin(mTOR) signal transduction pathway in rats with diabetic nephropathy(DN). Method: The 80 SD rats were divided into normal group, model group, irbesartan group (25 mg · kg-1 · d-1), Tonxinluo submicron powder group(0.8 g · kg-1 · d-1), Jinlida granules group(1.5 g · kg-1 · d-1) and Tonxinluo plus Jinlida group(0.8+1.5 g · kg-1 · d-1). Rats were injected with streptozotocin (STZ) by tail intravenous injection to establish diabetes mellitus (DM) models. The expression of vascular endothelial growth factors (VEGF) in renal tissues was detected with immunohistochemical assay of SP. The expression of Akt and mTOR proteins in Akt/mTOR signal transduction pathway as well as the expression of corresponding phosphorylated proteins p-Akt and p-mTOR were detected by Western blot. Result: The expression of VEGF was significantly enhanced in model group compared with the normal group, and the expression of VEGF in various medication groups was weakened by different degrees compared with the model group. The results of Tongxinluo submicron powder plus Jinlida granules group were more close to the results in normal group. There were no statistical differences in total protein expression of Akt and downstream molecules mTOR between all groups;while the expression of p-Akt and p-mTOR was significantly enhanced in the model group compared with the normal group (P<0.01), and the expression was significantly reduced in various medication groups (P<0.01). Conclusion: Tongxinluo submicron powder combined with Jinlida granules could suppress the activation of Akt/mTOR signal transduction pathway and reduce the expression of VEGF of relevant proteins in rats with DN.关键词：Tongxinluo;Jinlida;diabetic nephropathy;Akt/mTOR;signal transduction;vascular endothelial growth factors
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发布时间：2024-01-04 - 摘要：Objective: To observe the pharmacodynamic effect of Zijin Huadu suppository on treatment of cervicitis induced by virus, bacteria and phenol in vivo and in vitro, and provide experimental basis for evaluating its therapeutic effects for cervicitis. Method: The 70 SD rats were randomly divided into normal group, model group, Funing suppository group (0.15 g · kg-1 · d-1),policresulen suppository group (8.4 mg · kg-1 · d-1), Zijin Huadu suppository high dose group, middle dose group and low dose group (1.2, 0.6, 0.3 g · kg-1 · d-1), n=10 in each group. Needle scraping was done for three times in all the other groups except the normal group to induce surface damage of uterus, and infusion was done once every 2 days for 3 times in total to induce infection models. After successful modeling, vagina administering was done once a day for continuous 12 days. HE staining pathological dyeing was used to observe the cervicitis lesions of rats;ELISA assay was used to detect the expression of virus in cervical tissues;in vitro test was used to observe its inhibitory effect on herpes simplex virus-2 (HSV-2); RT-PCR and CPE method were used to observe the inhibitory effect of Zijin Huadu suppository on virus replication and its bactriostasis activity was observed by turbidimetry. Result: The results demonstrated that in HPV16-infected cervicitis rat models, Zijin Huadu suppositories at doses of 1.2, 0.6, 0.3 g · kg-1 · d-1had significant inhibitory effect on expression of HPV16 in cervical tissues, and the doses of 1.2, 0.6 g · kg-1 · d-1 had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models with mixed infection of Escherichia coli, Neisseria gonorrhoeae and Staphylococcus aureus, Zijin Huadu suppositories at doses of 1.2, 0.6 g · kg-1 · d-1 had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances, Zijin Huadu suppositories at dose of 1.2 g · kg-1 · d-1 had significant inhibitory effect on vagina and cervix lesions in rats. In vitro, Zijin Huadu suppository showed obvious inhibitory effect on HSV-2, HPV16, S. aureus, S. albus, S. epidermidis, group B Streptococcus, E. coli, Pseudomonas aeruginosa, Proteusbacillus vulgaris, Neisseria gonorrhoeae and Candida albicans. Conclusion: Zijin Huadu suppository has obvious inhibitory effect in vitro on viruses and bacteria, and has obvious improvement for all the phenol-induced cervicitis.关键词：Zijin Huadu suppository;Cervicitis;HPV16;bacteria mixed infection;phenol mucilage
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Saposhnikoviae Radix on protease-activated receptors-2 (PAR-2) expression and the related cytokine secretion of mast cells, and explore new anti-allergic mechanism of Saposhnikoviae Radix. Method: Mast cell degranulation models were established by stimulating P815 cells with trysin in vitro. Blank group, model group, Saposhnikoviae Radix high dose group and low dose group (0.02, 0.01 g · mL-1) were set up. After 6 h of treatment, ELISA assay was used to detect histamine, interleukin-4 (IL-4) and IL-13 levels in cell supernatant. Western blot and RT-PCR were used to detect PAR-2 protein and mRNA expression of P815 cells. Result: Compared with the blank group, histamine, IL-4, IL-13 levels and PAR-2 protein and mRNA expression were significantly increased in model group (P<0.05, P<0.01). Compared with model group, Saposhnikoviae Radix in vitro significantly inhibited the histamine, IL-4, IL-13 levels and PAR-2 protein and mRNA expression in mast cells (P<0.05,P<0.01). Conclusion: By reducing the expression of PAR-2, Saposhnikoviae Radix extract can inhibit mast cell degranulation, selectively decrease the secretion of related cytokines and then suppress the "waterfall effect" of mast cells, eventually resulting in anti-allergic effect.关键词：Saposhnikoviae Radix;protease-activated receptors-2;interleukin-4;interleukin-13
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发布时间：2024-01-04 - 摘要：Objective: To study the inhibition effect of compound Tufuling granules (CTG) on uric acid (UA), activity of xanthine oxidase (XO), and xanthine dehydrogenase(XDH) mRNA in hyperuricemic rats. Method: The 48 SD rats were randomly divided into normal group, model group, febuxostat group (4 mg · kg-1), CTG low-dose group, CTG mid-dose group and CTG high-dose group (1, 2, 4 g · kg-1). Rats in all other groups except normal group were used to establish hyperuricemia (HUA) models and received corresponding drugs for intervention, once a day for 5 days. After experiment, UA of serum, activity of XO, and XDH mRNA in liver were detected. Result: In model group, levels of UA, activity of XO, and XDH mRNA expression levels in liver were significantly higher than normal group (P<0.05). After treatment, levels of UA, activity of XO, and XDH mRNA expression levels were significantly lower in various treatment groups than those in model group (P<0.05), but there was no significant difference between CTG low dose group and model group. Conclusion: CTG may reduce the UA of serum, and inhibit the activity of XO and XDH mRNA expression level in hyperuricemic rats.关键词：compound Tufuling granules;hyperuricemia;xanthine oxidase;xanthine dehydrogenase mRNA
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of matrine (MAT) on the expression of chemokine ligand-2 (CCL2) and its receptor CCR2 mRNA in experimental autoimmune encephalomyetic (EAE) rats in the internationally accepted models of multipe sclerosis (MS), investigate the possible mechanism of MAT preventive treatment for EAE rats and provide clinical evidence. Method: The 50 Wistar female rats were divided into five groups randomly (n=10 each group):normal group, model group, MAT low-dose group (150 mg · kg-1), MAT high-dose group (250 mg · kg-1), and dexamethasone group (1 mg · kg-1). The MAT and dexamethasone were respectively injected intraperitoneally (ip) daily from day 0 post-immunization till to 17. At the same time, The model and normal group received the same amount of normal saline. The clinical symptoms were observed and recorded and the histopathological changes were detected. The expression levels of CCL2,CCR2 mRNA were detected using RT-PCR techniques. Result: Compared with model group, MAT obviously delayed the disease onset and ameliorated clinical symptoms of EAE (P<0.01), improved inflammatory infiltration (P<0.01) and demyelination (P<0.01) in central nervous system of EAE rats, and reduced the expression levels of CCL2/CCR2 mRNA in spinal cord (P<0.01, P<0.01). Conclusion: Matrine could prevent and treat EAE rats and the mechanism may be associated with reducing the expression of CCL2/CCR2 mRNA in spinal cord of EAE rats.关键词：matrine;multipe sclerosis;experimental autoimmune encephalomyelitis;chemokine ligand-2;chemokine ligand receptor-2
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发布时间：2024-01-04 - 摘要：Objective: To explore the electrophysiological effects of Erxian decoction on ovariextomized rat heart. Method: Thirty-two female healthy rats were randomly divided into four groups:sham operated control group, ovariectomized group, estrogen group, and Erxian decoction group. The rats in estrogen group and Erxian decoction group were intragastrically administrated with estradiol valerate (0.2 mg · kg-1 · d-1) and Erxian decoction (7.5 g · kg-1 · d-1) respectively, ig, for 12 weeks. The rats in sham operated control group and ovariectomized group were intragastrically administrated of with equivalent volume of purified water for 12 weeks. The Ⅱ lead ECGs of anesthetic rats were recorded. And the spontaneous activities of left ventricular outflow tract and the fast action potentials (APs) of left ventricular muscle were also recorded by intracellular electrode. Result: Compared with sham operated control group, P, R and T amplitude decreased notably, and P duration, PR interval, QT interval, and T duration decreased significantly in ovariectomized group (P<0.05, P<0.01). In estrogen group, T amplitude, P duration, PR interval and QT interval were reversed to control group. In Erxian decoction group, R amplitude, T amplitude, P duration and PR interval were reversed to control group. Compared with control group, the pacemaker rates of ventricular outflow tract and norepinephrine(NE) induced abnormal pacemaker activities increased significantly in ovariectomized group. In estrogen and Erxian decoction groups, the pacemaker activities of ventricular outflow tract decreased were markedly compared with lower than ovariectomized group, and NE-induced spontaneous arrhythmia was significantly relieved. That is, estrogen and er-xian decoction depressed the activity of latent pacemaker cell in ventricular outflow tract and had less possibility to lead to arrhythmia. Compared with control group, action potential duration (APD) was shortened significantly in ovariectomized group, especially APD50. Compared with ovariectomized group, APD50 was significantly prolonged in estrogen group and Erxian decoction group and restored to control group (P<0.05, P<0.01). Conclusion: Erxian decoction could significantly improve the electrophysiological activities of hearts of ovariectomized rats, heart and has a certain effect in relieving arrhythmia caused by estrogen reduction.关键词：Erxian decoction;ovariectomized rat;electrocardiography;ventricular outflow tract;action potential
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发布时间：2024-01-04 - 摘要：Objective: To discuss the interventional effect of Shuanghe Tang on alcohol-induced osteonecrosis of the femoral head (AOFH). Method: Totally 60 Chinese white rabbits were randomly divided into the normal group, the model group, the madopar group, and low, medium and high-dose Shuanghe Tang groups. Except for the normal group, all of the remaining groups were lavaged with Chinese liquor (alcohol content 56%) 10 mL · kg-1 · d-1. Meanwhile, each drug-treated group was lavaged with drugs. The normal group and the model group were given normal saline. After 8 weeks of reventive administration, the rabbits were sacrificed to collect blood for determination of the index of hemorheology, serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), bone glaprotein (BGP), vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2). The rabbits were killed after blood collection, and histopathological changes were detected. The expression levels of PPARγ mRNA and OC mRNA in femoral heads were analyzed by RT-PCR. Result: Compared with the normal group, the level of TC, TG, LDL and the index of hemorheology in the model group were significantly increased(P<0.01), and the levels of HDL, BGP, VEGF and BMP-2 were significantly decreased (P<0.01). Compared with the model group, the level of TC, TG, LDL and the index of hemorheology were significantly decreased in the medium and high-dose Shuanghe Tang groups, and the level of HDL, BGP, VEGF,BMP-2 and Osteoblast count were significantly increased (P<0.01). Compared with the normal group, the empty bone lacuna rate and average diameter of the max adipocyte in the model group were significantly increased(P<0.01),and the osteoblasts count was significantly decreased (P<0.01). Compared with the model group, the empty bone lacuna rate and average diameter of the max adipocyte were significantly decreased in the medium and high-dose Shuanghe Tang group (P<0.01), and the osteoblasts count was significantly increased (P<0.01). Compared with the normal group, the expression of PPARγ mRNA in the model group was significantly increased (P<0.01), and the expression of OC mRNA was significantly decreased (P<0.01). Compared with the model group, the expression of PPARγ mRNA was significantly decreased in the medium dose Shuanghe Tang group (P<0.01), and the expression of OC mRNA was significantly increased(P<0.0). Conclusion: Shuanghe Tang can effectively relieve alcohol-induced hyperlipidemia, improve hemorheology and reduce blood viscosity, inhibit adipogenic differentiationof marrow stromal cells induced by alcohol, and maintain osteogenic differentiation in femoral head in rabbits, so as to prevent and treat AOFH.关键词：alcohol-induced osteonecrosis of femoral head;Shuanghe Tang;lipid;blood viscosity;gene
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of resveratrol (Res) on MGC803 cells apoptosis and study its mechanism of inhibiting gastric cancer. Method: After MGC803 cells were treated with 0, 50, 100, 200 μmol · L-1 Res, inhibition rate of MGC803 cells growth was detected by trypan blue assay. Apoptosis rate was detected by flow cytometry (FCM). Expressions of Bad and Caspase-3 protein in MGC803 cells were measured by immunohistochemistry method. Expressions of Bad, p-Bad, Caspase-3 protein were detected by Western blot. Result: Res inhibited the growth of MGC803 cells in time-and concentration-dependent manner (P<0.01). Res (100 μmol · L-1) can significantly down-regulate the protein expressions of Bad, p-Bad and up-regulate the protein expression of Caspase-3 in MGC803 cells. Conclusion: Res can induce apoptosis of MGC803 cells in time-and concentration-dependent manner, and the mechanism may be associated with down-regulating protein expressions of Bad, p-Bad and up-regulating protein expressions of Caspase-3.关键词：resveratrol;MGC803 cells;apoptosis;Bad protein;p-Bad protein;Caspase-3 protein
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of irisquinone for radiation-inducedlung injury (RILI) in SD rats. Method: Two hundred and ten SD rats were randomly divided into 0.9% sodium chloride solution group (normal group),irisquinone group (single drug group), radiation group (model group), hormone group, high-dose (60 mg · kg-1 · d-1), medium-dose (30 mg · kg-1 · d-1), and low-dose (15 mg · kg-1 · d-1) irisquinone+radiation group, n=30 in each group. The models of RILI in SD rats were established by irradiating right whole chest with a single dose of 20 Gy using 60Co γ therapeutic machine. Rats were sacrificed from the seven groups at week 1, 4, 8, 12 and 24 post-irradiation, and the pathological changes of the lung were observed by hematoxylin-eosin(HE) staining and Masson staining; content of hydroxyproline in lung tissues was detected by UV spectrophotometer, tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and transforming growth factor β1(TGF-β1) in serum were detected by enzyme-linked immunosorbent assay, expression of TNF-α and TGF-β1 in lung tissues were detected by immunohistochemical method. Result: High-dose and medium-dose irisquinone+radiation group could significantly ameliorate radiation-inducedlung injury, effectively reduce the content of hydroxyproline and inhibite the expression of TNF-α, IL-1β and TGF-β1, with statistically significant difference compared with model group (P<0.05). There was no significant difference between the low-dose irisquinone+radiation group and the model group. There was no statistically significant difference between the single drug group and the normal group. Conclusion: The high-dose and medium-dose irisquinone has protective effect on the radiation-induced lung injury and is nearly non-toxic for the lung tissues of rats.
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发布时间：2024-01-04 - 摘要：Objective: To explore the acute toxicity and genotoxicity of Dipsaci Radix in mice. Method: The acute toxicity of Dipsaci Radix in mice was evaluated by the maximum tolerated dose method, and the changes of body weight, heart,spleen,liver,and kidney indexes were observed. Its genotoxicity was evaluated by micronucleus test and single cell gel electrophoresis test. 40 mice were divided into five groups randomly:blank group (normal saline of 10 mL · kg-1 by gavage), cyclophosphamide group (0.1 g · kg-1 cyclophosphamide was injected since the fourth day),and Dipsaci Radix water extract liquid high dose group, middle dose group and low dose group (32,16 and 8 g · kg-1 by gavage). 5 days latter,all of the mice were sacrificed and anatomized.The bone marrow cells were taken to detect the micronucleus rate of polychromatic erythrocytes,and the DNA damages of thymus,liver and spleen were detected by single cell gel electrophoresis test. Result: The maximum tolerated dose was 80 g · kg-1 for Dipsaci Radix water extract liquid by oral taking in mice,and such dose had no significant effect on body weight and heart,spleen,liver,and kidney indexes. Compared with blank group, there was no significant difference in micronucleus rate of polychromatic erythrocytes in middle and low dose groups, but the micronucleus rate of polychromatic erythrocytes in high dose group was significantly higher than that in blank group (P<0.05).The tail DNA percent and tail moment of thymus,liver and spleen in high, middle and low dose groups were a little higher than those in blank group, but with no significant difference. Conclusion: The maximum tolerated dose of Dipsaci Radix water extract liquid is 80 g ·kg-1 in mice, with no acute toxicity on mice. Middle and low doses of Dipsaci Radix water extract liquid have no genotoxicity on mice, while high dose of Dipsaci Radix has increased the micronucleus rate of polychromatic erythrocytes, showing potential genotoxicity on mice.关键词：Dipsaci Radix;acute toxicity;genotoxicity;micronucleus;single cell gel electrophoresis
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of oridonin on inhibiting angiogenesis. Method: In vitro and in vivo models were used in this paper to study the anti-angiogenic effects of oridonin. Through in vitro models, methyl thiazolyl tetrazolium (MTT) assay was ysedti detect the cells vitality and enzyme-linked immunosorbent assay (ELISA) was used to determine vascular endothelial growth factors level (VEGF) in cardiac microvascular endothelial cells (CMEC). Through in vivo models, transgenic zebrafish models (Fli1-GFP) were used to observe the effect of oridonin on angiogenesis of embryonic period and regeneration after vascular injury in adult zebrafish. Expressions of main related genes VEGFA, VEGFR2 and VEGR3 in VEGF pathways were detected with relative fluorescence quantitative PCR method. Result: Oridonin inhibited angiogenesis and endothelial cells in vitro, with IC50 of 8.04 mg · L-1, and significantly reduced VEGF expression in the cell serum. What's more, oridonin inhibited angiogenesis in zebrafish embryos vascular assay and inhibited regeneration after vascular injury in adult zebrafish. Our study elucidated the mechanism of the anti-angiogenic activity of oridonin at least in part, which may be related to down-regulating the expression of VEGFA, VEGFR2 and VEGR3 genes. Conclusion: Oridonin can effectively inhibit angiogenesis, which will provide greater contribution for anti-tumor angiogenesis therapy.关键词：Oridonin;cardiac microvascular endothelial cells;zebrafish;vascular endothelial growth factorA;vascular endothelial growth factor R2;vascular endothelial growth factor R3
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of capsaicin on the invasion ability of human large cell carcinoma NCI-H460 and the expressions of E-cadherin and Slug, in order to discuss the possible mechanisms of anti-non-small cell lung cancer. Method: NCI-H460 cells were cultured in vitro and treated with capsaicin with various concentrations, with no capsacin in the blank group. The 50% inhibitory concentration(IC50) was measured by MTT method. The NCI-H460 cell invasion ability was evaluated by actin-tracker green fluorescent probe and transwell chamber invasion assay. Western blot and RT-PCR were used to detect the protein and mRNA expression of E-cadherin and Slug. Result: MTT assay results showed that the growth of NCI-H460 cells treated with capsaicin was significantly inhibited compared with the blank group, with statistically significant difference (P<0.05);Actin-tracker Green fluorescent probe showed that capsaicin can notably prohibit NCI-H460 filopodia formation. Transwell invasion in vitro results showed that capsaicin can significantly inhibit invasion of penetrating cells, with statistically significant difference compared with the control group (P<0.05). Western blot and RT-PCR analysis showed that E-cadherin expression level was significantly elevated and Slug expression level was significantly decreased by capsaicin, with statistically significant difference compared with the control group (P<0.05). Conclusion: Capsaicin can inhibit the NCI-H460 cell viability and invasion, its mechanism for anti-non-small cell lung cancer may be correlated with decrease in Slug expression and increase in E-cadherin expression.关键词：capsaicin;invasion ability;E-cadherin;Slug
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of puerarin on acute lung injury in rats. Method: Sixty Wistar rats were randomly divided into normal group, model group, dexamethasone group (3 mg · kg-1), high-, medium-, low-dose (10, 5, 2.5 mg · kg-1) puerarin groups, with 10 rats in each group. Rats were intragastrically gavaged with drugs for 7 days. Normal group and model group were given normal saline. Thirty minutes after last gavage, the acute lung injury rat model was induced through intraperitoneal injection with 20% yeast suspension every 12 h for twice. After 24 h, lung tissues were removed and stained with HE for pathological observation. The wet and dry proportion, superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px) and malonaldehyde (MDA) in lung tissue were determined. Serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) contents were detected with ELISA method. The protein expression of nuclear factor-κB (NF-κB) was measured using Western blot. Result: Compared with model group, model group showed significant increases in wet and dry proportion of lung tissues, MDA, TNF-α, IL-6, NF-κB contents and decreases in SOD and GSH-Px levels (P<0.05, P<0.01). Compared with model group, puerarin groups showed significant decreases in wet and dry proportion of lung tissues, MDA, TNF-α, IL-6, NF-κB contents and increases in GSH-Px levels (P<0.05), with alleviations in lung hemorrhage and cellular infiltration. Conclusion: The inflammatory response and pulmonary edema in lung tissues can be relieved in acute lung injury rats by puerarin intervention, andn its protective mechanism is associated with antioxidative stress by lowing TNF-α, IL-6, NF-κB levels.关键词：puerarin;acute lung injury;nuclear factor-κB;antioxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To compare and observe the clinical effect of dispensing granules and traditional decoction of coronary heart disease mixture on stable angina pectoris of coronary heart disease, Qi-stagnancy and blood stasis, and stagnation of Yang in chest. Method: The 252 patients were randomly divided into control group (n=126) and treatment group (n=126). Patients in treatment group were treated with dispensing granules of coronary heart disease mixture and routine west medicine; patients in control group were treated with traditional decoction of coronary heart disease mixture and routine west medicine. Before treatment, at week 2 of treatment, week 4 of treatment and after treatment (week 6), traditional Chinese medicine(TCM) syndrome efficacy scores, Seattle angina scale scores, the changes of electrocardiogram(ECG), blood lipid, blood rheology, left ventricular ejection fraction, nitroglycerin usage and other indexes were observed and compared. Symptoms of TCM syndrome, curative effect of Chinese and Western medicine, as well as quality of life were compared respectively. Result: Coronary heart disease mixture had good effect on patients with different degrees of angina pectoris. Total effective rate was 88.0% in treatment group (granule form) and 76% in the control group (traditional decoction form).Effect of different forms had no significant difference for mild and severe patients, but dispensing granules had more prominent effect for patients with moderate conditions. This may be related to its stability and content purity as well as the objective factors such as daily life of patients, requiring further deep research. Conclusion: Coronary heart disease mixture's dispensing granules have better efficacy than traditional decoction form, with certain clinical popularization value. It provides strong support for the improvement of Chinese medicine in the treatment of coronary heart disease and stable angina pectoris, not only adapts to the increasingly fast pace of work and life, but also provides overall solution for the application of TCM in modern society.关键词：coronary heart disease mixture;stable angina pectoris of coronary heart disease;dispensing granules;comparative study of the clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To explore clinical efficacy of Tongxie Anpi decoction combined with trimebutine maleate tablets for treating diarrhea-predominant irritable bowel syndrome (IBS-D) with hepatic stagnation and splenic deficiency as well as its effect on 5-hydroxytryptamine (5-HT) and calcitonin gene related peptide (CGRP). Method: The 80 patients were randomly divided into treatment group and control group (n=40 in each group). Patients in two groups were both given with trimebutine maleate tablets treatment, and Tongxie Anpi decoction was added in the treatment group. Therapeutic course was 4 weeks for both groups. Changes in curative effect of traditional Chinese medicine (TCM) syndrome and TCM symptom scores were observed for both groups. Serum levels of 5-HT and CGRP of patients in two groups were observed before and after treatment. Result: Total effective rate of TCM syndromes was 87.5% in treatment group, superior to 65.0% in control group (P<0.05). In comparison of TCM symptom scores before and after treatment in two groups, differences were statistically significant except bowel distress and mucous stool in control group (P<0.05, P<0.01), and improvement of diarrhea, abdominal pain, abdominal distension, bowel distress and mucous stool in treatment group was superior to that in control group (P<0.05, P<0.01). Serum levels of 5-HT and CGRP in two groups were significantly decreased after treatment (P<0.05, P<0.01), and decrement of serum levels of 5-HT and CGRP was significantly larger in treatment group than that in control group (P<0.05). Conclusion: Tongxie Anpi decoction combined with Trimebutine Maleate tablets has clinical effect in treating diarrhea-predominant irritable bowel syndrome with hepatic stagnation and splenic deficiency, and can obviously improve the patients' symptoms. Mechanism may be associated with reducing patients' serum levels of 5-HT and CGRP.关键词：diarrhea-predominant irritable bowel syndrome;hepatic stagnation and splenic deficiency;Tongxie Anpi decoction;5-hydroxytryptamine;calcitonin gene related peptide
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发布时间：2024-01-04 - 摘要：Objective: To investigate efficacy of Xiaoyin decoction combined with narrow band ultraviolet (NB-UVB) in treatment of psoriasis vulgaris of blood-heat type and its effect on expression levels of interleukin-17 (IL-17), interleukin-23 (IL-23) and interleukin-6 (IL-6) of peripheral blood. Method: The 60 patients with psoriasis vulgaris of blood-heat type were randomly divided into treatment group and control group, n=30 in each group. Treatment group was treated with Xiaoyin decoction combined with NB-UVB and control group was treated with NB-UVB alone. The treatment course was 8 weeks for both groups. Another 30 healthy volunteers served as the normal control group. Expression levels of IL-17, IL-23 and IL-6 in healthy control group and patients with psoriasis vulgaris were detected before and after treatment, and Psoriasis Area and Severity Index (PASI) scores were recorded respectively before treatment, 4 weeks after treatment and 8 weeks after treatment. Result: Effective rate was 83.3% in treatment group, higher than 56.7% in control group (P<0.05). PASI score was (3.30±1.13) in treatment group and (6.00±1.10) in control group after treatment, with statistically significant difference (P<0.01). Expression levels of IL-17, IL-23 and IL-6 in treatment group and control group were significantly higher than those of healthy control group before treatment (P<0.01);there was statistically significant difference in expression levels of IL-17, IL-23 and IL-6 before and after treatment in both groups (P<0.01). After treatment, there was statistically significant difference in above indicators between treatment group and control group (P<0.05, P<0.01). There was positive correlation between serum levels of IL-17, IL-23, IL-6 and PASI scores in treatment group and control group. Conclusion: Xiaoyin decoction combined with NB-UVB had better effect than NB-UVB treatment alone, and mechanism may be associated with decreasing serum expression levels of IL-17, IL-23 and IL-6 in Th17 cells.关键词：psoriasis;Xiaoyin decoction;narrow band ultraviolet;interleukin-17;interleukin-23;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Banxia Xiexin Tang in treatment of helicobacter pylori(Hp)-associated peptic ulcer with syndrome of intermingled heat and cold and observe its effect on serum levels of osteopontin (OPN) and β-catenin. Method: One hundred and ten patients with Hp-associated peptic ulcers at internal digestive wards of our hospital were randomly divided into treatment group and control group (55 cases in each group) referring to random number table. Patients in both groups were given with non-drug therapy with reference to "Diagnosis and Treatment Guidelines for Peptic Ulcer Disease". Patients in control group received therapy of proton pump inhibitors (PPI),including omeprazole enteric-coated tablets (20 mg, bid), clarithromycin tablets (500 mg, bid) and amoxicillin capsules (0.5 g, bid). The above drugs were orally taken for 2 weeks. After 2 weeks, Pantoprazole sodium enteric-coated tablets (40 mg, qd) were orally taken for 2 weeks. Patients in treatment group were also treated with Banxia Xiexin Tang (1 dose/d, boiled twice conventionally) for 4 weeks in total based on the treatment in control group. The main symptoms and scores of gastroduodenal mucosa injury were compared before and after treatment in both groups. Hp elimination rates and the clinical curative effects were analyzed for both groups. Serum levels of OPN and β-catenin were detected. Result: Scores of clinical symptom for syndrome of intermingled heat and cold in treatment group were significantly lower than those of control group after treatment (P<0.01). The total effective rate was 96.36% in treatment group, superior to 80% in control group, with statistical differences (P<0.05). After treatment,Hp elimination rate in treatment group was 90.91%, superior to 74.45% in control group, with statistical differences (P<0.05). Scores of gastroduodenal mucosa injury in treatment group were significantly lower than those in control group after treatment (P<0.01). Serum levels of OPN and β-catenin in treatment group were significantly higher than those in control group after treatment, with statistically significant differences (P<0.01). Conclusion: Based on the routine non-drug therapy and triple therapy, Banxia Xiexin Tang add and subtract for Hp-associated peptic ulcer could effectively improve the main clinical symptoms and gastroduodenal mucosa lesions, and increase Hp elimination rates and the clinical curative effects. The mechanism above may be relate to inhibiting serum levels of OPN and β-catenin.关键词：Banxia Xiexin Tang;helicobacter pylori;syndrome of intermingled heat and cold;osteopontin;β-catenin
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Maxin Shigan Tang combined with Tingli Dazao Xiefei Tang as modified auxiliary therapy for community acquired pneumonia (CAP) in middle-aged and old patients as well as its effect on C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and procalcitonin (PCT). Method: One hundred and six cases patients with CAP were divided into control group (52 cases) and experimental group (54 cases) by random number table. Patients in control group received cefoperazone sodium and sulbactam sodium for intravenous infusion, 3 g, 2 times/day and azithromycin injection for intravenous infusion, 0.5 g, 1 time/day, with a treatment course of 14 days in total. Based on the treatment of control group, patients in experimental group added Maxin Shigan Tang combined with Tingli Dazao Xiefei Tang, 1 dose/day, with a treatment course of 14 days. Before and after treatment, scores of main symptoms and signs were recorded;fevers, cough, expectoration, lung rale, lung slices, the blood and average stay were recorded. Before and after treatment, levels of CRP, ESR and PCT were detected. Result: In Ridit analysis, clinical effect in experimental group was superior to that in control group (P<0.05). After treatment, scores of the main symptoms and sings (cough, expectoration, sputum, wheeze, breathe hard, flustered and exasperated, lags in response, moist rales, excessive phlegm and bronchophony) were lower than those in control group (P<0.01). After treatment, time of fevers, cough, lung rale, recovery time in lung slices and blood examination as well as the average stay in experimental group were shorter than those in control group (P<0.05, P<0.01). Levels of CRP, ESR and PCT were also lower than those in control group after treatment (P<0.05, P<0.01). Conclusion: Maxin Shigan Tang combined with Tingli Dazao Xiefei Tang can relieve symptoms and signs, shorten courses, reduce levels of CRP, ESR and PCT, and improve clinical effect for the treatment of community acquired pneumonia (CAP) in middle-aged and old patients.关键词：community acquired pneumonia;Maxin Shigan Tang;Tingli Dazao Xiefei Tang;procalcitonin
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Buqi Jiannao Tongluo Tang combined with acupuncture in improving ischemic stroke (syndrome of blood stasis due to qi deficiency) at recovery phase and its effect on serum S-100B protein (S-100B) and homocysteine(Hcy). Method: Ninety-six cases patients with ischemic stroke were randomly divided into acupuncture group (48 cases) and observation group (48 cases) according to their hospitalization sequence. All cases were given with conventional medical treatment and rehabilitation training for limbs. Patients in acupuncture group received the therapy of acupuncture (3 times/week). On the basis of acupuncture group,the patients in treatment group were also treated with Buqi Jiannao Tongluo Tang (1 dose/day,bid) with conventional water frying. The treatment course was 8 weeks for both groups. The clinical efficacy was evaluated by Brunstrom method. Muscle strength and Fugl-Meyer motor scale(FMMS) for upper and lower limbs,activities of daily living (BI index),Berg balance scale(BBS) and walking ability were assessed before and after treatment in both groups. Serum levels of S-100B and Hcy were detected before and after treatment in both groups. Result: The total effective rate was 87.5% in observation group, superior to 68.75% in acupuncture group (P<0.05). After treatment, muscle strength scores for upper and lower limbs in observation group were significantly lower than those in acupuncture group(P<0.01),while FMMS scores were significantly higher than those in acupuncture group(P<0.01). After treatment, BI and BBS scores in the observation group were significantly higher than those of acupuncture group (P<0.01), and score for walking ability of observation group was lower than that in acupuncture group after treatment (P<0.01). S-100B and Hcy serum levels in observation group were significantly lower than those of acupuncture group after treatment with statistically significant difference between two groups (P<0.01). Conclusion: On the basis of conventional medical treatment and rehabilitation training for limbs,Buqi Jiannao Tongluo Tang combined with acupuncture can improve muscle strength and motor function for upper and lower limbs,activity of daily living,ability of balance,and walking ability,and reduce serum levels of S-100B and Hcy for the treatment of ischemic stroke (syndrome of blood stasis due to qi deficiency) at recovery phase.关键词：ischemic stroke;Buqi Jiannao Tongluo Tang;acupuncture;syndrome of blood stasis due to qi deficiency;motor function
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Yishen Huashi granules combined with sulodexide on oxidative stress and endothelial function in early diabetic nephropathy of elder patients. Method: The 86 patients with early diabetic nephropathy were randomly divided into control group and observation group. Both groups were given with conventional therapy. The patients in control group were also treated with sulodexide intramuscular injection 600 LSU, once daily;after continuous treatment for 2 weeks, sulodexide soft capsules were orally taken, 1 capsule/time, 2 times daily, and continuous for 4 months. The patients in observation group were also treated with Yishen Huashi granules based on the treatment of control group,10 g/time, 3 times daily, and continuous for 4 months. Blood glucose, serum creatinine (SCr), superoxide dismutase (SOD), malondialdehyde (MDA), vascular pseudo von Willebrand factor (vWF), endothelin (ET)-1 and 24 h urinary albumin excretion rate (24 h UAER) were observed before and after treatment. Result: The levels of 24 h UAER and SCr in the two groups were significantly decreased after treatment (P<0.05, P<0.01). The levels of 24 h UAER and SCr in the observation group were (72.3±15.8) mg · (24 h)-1, and (65.8±20.6) μmol · L-1, significantly lower than (87.6±17.2) mg · (24 h)-1, (91.6±23.8) μmol · L-1 in the control group, with statistically significant difference (P<0.05). The levels of MDA, vWF, and ET-1 in the observation group were (4.01±0.4) μmol · L-1, (159.5±26.4)%, and (55.7±7.2) ng · L-1 after treatment, significantly lower than those before treatment (P<0.05) and significantly lower than (5.31±0.51) μmol · L-1, (181.3±28.2)%, and (60.4±7.5) ng ·L-1 in the control group after treatment, with statistically significant difference (P<0.05). The level of SOD in the observation group was (129.3±7.1) U · mL-1after treatment, significantly higher than the level before treatment (P<0.05) and significantly higher than (70.2±4.5) U · mL-1in the control group after treatment, with statistically significant difference (P<0.05). Conclusion: Yishen Huashi granules have strong antioxidant effect, which can effectively delay the vascular endothelial injury and reduce urinary protein excretion. Yishen Huashi granules combined with sulodexide have more obvious effect on regulating vascular endothelial function and delaying the development of early diabetic nephropathy.关键词：diabetic nephropathy;Yishen Huashi granules;sulodexide;oxidative stress;endothelial function
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发布时间：2024-01-04 - 摘要：Objective: To explore the potential mechanism of Tianfoshen oral liquid in the treatment of colorectal cancer. Method: Software of Arrowsmith based on the theory of Swan's noninterative literature-bases knowledge discovery was applied. Result: The preliminary investigation results by Arrowsmith software showed that the effective components of Tianfishen oral liquid might havecertain ability to regulate the expressions of toll-like receptors (TLRs), vascular endothelial growth factor (VEGF),cyclooxygenase-2 (COX-2), and apoptosis related factors, and the abnormal expressions of the above indicators were also the key genetic factors for colorectal cancer. Conclusion: Tianfishen oral liquid has significant effect on inhibiting colorectal cancer in vitro and in vivo, and the mechanism may be associated with down-regulating the expressions of toll-like receptor 4 (TLR4) and COX-2 in colorectal cancer tissues, reducing abnormally high expression of VEGF in tissues, and promoting expression and vitality of apoptosis genes. This can provide strong reference for the laboratory to conduct further mechanism verification studies.关键词：Tianfoshen oral liquid;effective components;colorectal cancer;Arrowsmith
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发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy and safety of paclitaxel liposome injection and common paclitaxel in the treatment of gastric cancer. Method: Pubmed, EBM, EMBase, Cochrane Library, CNKI and other related database were retrieved for randomized controlled trials (RCTs) on the paclitaxel in treatment of gastric cancer, limited to Chinese and English versions. Two reviewers screened the literature and extracted data independently according to the inclusion and exclusion criteria, and then used the Jadad scale to evaluate the quality of literature. Result: The 6 RCTs, 355 patients in total were included for paclitaxel liposome VS common paclitaxel in the treatment of gastric cancer. Meta-analysis results showed that paclitaxel liposome had higher effective rate in the treatment of gastric cancer [OR=1.42, 95%CI (0.92, 2.18), P=0.11], and higher benefit rate [OR=1.92, 95%CI (1.14, 3.22), P=0.01], with statistical difference in benefit rate. In respect of safety, paclitaxel liposome caused fewer adverse reactions such as joint pain [OR=0.09, 95%CI (0.04, 0.36), P<0.000 01], muscle pain [OR=0.07, 95%CI (0.01, 0.36), P=0.001], facial flushing [OR=0.06, 95%CI (0.01, 0.26), P=0.000 2] than common paclitaxel, with statistically significant difference. Conclusion: Paclitaxel liposome has higher benefit rate and more obvious effect than common paclitaxel in treatment of gastric cancer, and meanwhile, it can significantly alleviate the toxic side effects and improve the care experience for patients.关键词：paclitaxel liposome;paclitaxel;gastric cancer;systemic evaluation
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发布时间：2024-01-04 - 摘要：Objective: To systematically evaluate the clinical efficacy of Longdan Xiegan Tang (pill) for herpes zoster. Method: CNKI, VIP and Wanfang database were retrieved for the published literature on Longdan Xiegan Tang (pill) to treat herpes zoster with randomized or semi-randomized controlled trials to Mar of 2015.The studies which met the criteria were selected, and the quality evaluation and Meta statistical analysis were done using RevMan 5.3 software. Result: A total of 15 trials met the inclusion criteria, and Meta analysis showed that the relative risk (RR) and 95% confidence interval of total efficiency were 1.16 (1.12,1.21);MD values (95%CI) of blister stopping, incrustation, analgesia and heal time in the symptoms and signs were [-0.83 (-1.47,-0.18),-0.47(-0.76,-0.18),-0.62(-1.08,-0.16),-1.25(-2.07,-0.44)]; OR (95%CI) of postherpetic neuralgia after treatment was [0.13(0.05, 0.36)]. Conclusion: Compared with the western medicine, Longdan Xiegan Tang (pill) has high curing rate for herpes zoster, and has obvious advantages in improving the symptoms and signs and postherpetic neuralgia, but the low quality of included clinical research influences the reliability of the systematic review.关键词：Longdan Xiegan Tang (pill);herpes zoster;systematic review
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发布时间：2024-01-04 - 摘要：Objective: To analyze the composition rules of prescriptions in treating recurrent oral ulcer in the literature in Chinese journal full-text database (CNKI) using the Traditional Chinese Medicine Inheritance Support System, and provide reference for further research and development of new traditional Chinese medicines(TCM) in treating recurrent oral ulcer. Method: The Traditional Chinese Medicine Inheritance Platform Software V2. 5 was used to build a prescription database of Chinese medicines in treating recurrent oral ulcer. The software integration data mining method was used to distribute prescriptions according to four natures of drugs, five flavours and meridians in the database and achieve frequency statistics, syndrome distribution, prescription rules and new prescription analysis. Result: Analysis on 158 prescriptions in treating recurrent oral ulcer determined the frequencies of herbs, commonly used herb pairs and combinations in prescriptions, and summarized 11 new prescriptions. Conclusion: This study indicated that the prescriptions in treating recurrent oral ulcer in Chinese journal full-text database are mostly those for heat-clearing and detoxifying, heart-relieving and blood cooling, spleen and kidney warming, soothing liver-Qi stagnation, with a cold or warm property, bitter and sweet in taste. TCM syndromes can be divided into accumulated heat in spleen and stomach, hyperactivity of fire due to Yin deficiency, flaming up of heart fire, deficiency of both Qi and blood,Yang deficiency of spleen and kidney, syndrome of Pi-deficiency with damp harassment, depressing internal heat in liver, and stagnation of Qi and blood stasis,etc.关键词：recurrent oral ulcer;Traditional Chinese Medicine Inheritance System;prescription;composition rules in prescriptions
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