最新刊期
卷 22 ， 期 2 ， 2016
- 摘要：Objective:To compare chemical composition change in Gardeniae Fructus before and after being processed by fingerprint,and compare content difference of five constituents (geniposide,genipin-1-β-D-gentiobioside,crocin-Ⅰ,crocin-Ⅱ,crocin-Ⅲ) in Gardeniae Fructus. Method:HPLC separation of five constituents was performed on Hypersil ODS C18 column (4.6 mm×250 mm,5μm) with mobile phase of acetonitrile-water (containing 0.2% phosphoric acid) for gradient elution,a flow-rate of 1.0 mL·min-1,injection volume of 5μL,column temperature at 30℃ and detection wavelengthes of 238,440 nm.Chromatograms of 30 batches of samples were compared by software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2004A). Result:Compared with raw products of Gardeniae Fructus,five constituents in processed products were all changed in varying degrees,and one new chromatographic peak were found after processed. Conclusion:Before and after being processed,Chemical composition change in Gardeniae Fructus may be major elements to affect its efficacy and drug properties.关键词：Gardeniae Fructus;processed products with Zhangband;HPLC;chemical composition
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发布时间：2024-01-04 - 摘要：Objective:To optimize preparation process of Jieyu Shuxin granules and set up its quality standard. Method:According to characteristics of granules,wet granulation was employed,kinds and amounts of excipients in granules were optimized with indexes of solubility,clarity and powder ratio.With qualified rate of particle size as index,orthogonal test was adopted to optimize formulation process by taking ethanol concentration,amounts of dextrin and xylitol as factors.HPLC-ELSD was employed to determine contents of jujuboside A and B in this granules with acetonitrile-water (35:65)as mobile phase and column temperature at 35℃. Result:Xylitol had the best clarity with minimum powder ratio.Optimum recipe ratio was dry extract-xylitol-dextrin (0.5:15:7),50% ethanol as a adhesive.Contents of jujuboside A and B in Jieyu Shuxin granules were 0.683,1.369 mg·g-1,respectively. Conclusion:These methods are proved to be reliable,sensitive and specific,which can be used in quality control of Jieyu Shuxin granules.关键词：Jieyu Shuxin granules;qualified rate of particle size;angle of repose;Ziziphi Spinosae Semen;total saponins
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发布时间：2024-01-04 - 摘要：Objective:To cover up bad taste of paeonol and prepare paeonol common melting substance granules,and then investigate in vitro dissolution of this granules. Method:Taking glyceryl behenate as masking material,common melting substance granules were prepared by melting quenching method.With proportion of paeonol and glyceryl behenate,the amount of PEG6000 and particle size as factors,orthogonal test was adopted to optimize formulation technology by taking taste masking effect and dissolution in 60 min as comprehensive evaluation index.Dissolution of paeonol was determined by UV spectrophotometry. Result:The best formulation process of paeonol common melting substance granules was as following:paeonol-glyceryl behenate (1:3.5),the amount of PEG6000 accounted for 13% of total content of paeonol and glyceryl behenate,particle size of 80-100 mesh.Particles size had a significant impact for taste masking effect and dissolution of paeonol common melting substance granules,dissolution of paeonol in 60 min was more than 70%. Conclusion:Paeonol common melting substance granules has good taste masking effect.This optimized process is simple and feasible.关键词：paeonol;taste masking technology;common melting substance granules;dissolution;glyceryl behenate
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发布时间：2024-01-04 - 摘要：Objective:To investigate effects of ultrafiltration process on function of Shentong Zhuyu Tang and investigate feasibility of ultrafiltration technology for purification of this decoction. Method:Painful models were established with hot plate test or acetic acid writhing,inflammatory model were established with daubing dimethylbenzene on auricle.Pain threshold improvement rate of mice,latency of body-writhing,frequency of body-writhing response in 15 minutes and mice auricle swelling inhibition rate were used as indexes to observe effects of ultrafiltration process on analgesic and anti-inflammatory activity of Shentong Zhuyu Tang. Result:Analgesic and anti-inflammatory effects of Shentong Zhuyu Tang was remarkable,the largest percentage of pain threshold extension in mice rose to (106.2±28.1)%,writhing incubation rose to (355.1±42.8) s,number of writhing in 15 minutes rose to 30.8±7.7,auricle swelling inhibitory rate rose to 41.27%,significant difference in all indexes was seen as compared to the control group.No significant difference in all indexes was seen between groups of the non-ultrafiltration processed and ultrafiltration processed by 20,50 nm ultrafiltration membranes. Conclusion:Ultrafiltration technology can be applied to purify Shentong Zhuyu Tang,membrane pore size must be not smaller than 20 nm.关键词：Shentong Zhuyu Tang;ultrafiltration;analgesic and anti-inflammatory activtity;latency;swelling degree
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发布时间：2024-01-04 - 摘要：Objective:Based on copy corresponding pathologic model,to discuss rationality of processing by comparing pharmacodynamics of Pingwei San. Method:Rats were divided into seven groups randomly,such as the normal group,the model group,the positive group,Pingwei San group,Pingwei San with processed Glycyrrhizae Radix et Rhizoma group,Pingweisan with processed Atractylodis Rhizoma group and Pingwei San with processed products group.In addition to the normal group,models caused by improper dieting,excessive external dampness and emotional discomfort with the spleen and stomach being stranded by dampness.Propulsive rate of the small intestine,gastric residual rate and swimming exhaustion time were taken as evaluation indexes for treatment of Pingwei San. Result:Compared with the normal group,gastric residual rate,propulsive rate of the small intestine and anti-fatigue capability of the model group decreased significantly,which manifested the model was successful.Compared with the model group,each treatment group had a significant improvement effect,while the positive group and Pingwei San with processed products group had the greatest effect. Conclusion:After Glycyrrhizae Radix et Rhizoma and Atractylodis Rhizoma in Pingwei San being processed,this prescription is preferable for improving dampness obstructing spleen-stomach syndrome.关键词：Pingwei San;spleen and stomach being stranded by dampness;pharmacodynamics;Atractylodis Rhizoma;propulsive rate of the small intestine;gastric residual rate
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of volatile oil in Chuangui Huoxiang granules and investigate its pharmacodynamics. Method:Taking yield of volatile oil as index,orthogonal test was adopted to optimize extraction technology of volatile components with extraction time,pressure and temperature as factors.Analgesic and anti-inflammatory effects of this granules was studied by hot plate analgesia experiment,writhing test and ear swelling experiment. Result:Optimum extraction conditions were as follows:extraction pressure of 25 MPa,temperature at 50℃ and lasting for 1.5 h;yield of volatile oil was 3.83 mg·g-1.Chuangui Huoxiang granules had analgesic and anti-inflammatory effects which prepared by the new process or the old process,and efficacy of the former was stronger than the latter. Conclusion:This optimized extraction process is reasonable,stable and suitable for industrial production of Chuangui Huoxiang granules,this granules can prolong pain threshold of mice,decrease writhing response of mice and inhibit ear swelling degree in mice.关键词：Supercritical CO2 extraction method;volatile oil;Chuangui Huoxiang granules;hot plate analgesia test;writhing test
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发布时间：2024-01-04 - 摘要：Objective:To optimize clarification technology of Yinma oral liquid. Method:A logarithmic equation for turbidity determination was established.Orthogonal test was adopted to investigate effects of chitosan dosage,pH value,clarification temperature and time on overall desirability of the content of chlorogenic acid,solid content and turbidity.Particle size and Zeta potential was used for stability analysis. Result:Optimal clarification technology conditions was as follows:chitosan dosage of 0.1%,stirring time of 10 min,pH 4.5,heated for 2 h at 40℃ and refrigerated 12 h at 4℃.The content of chlorogenic acid was 0.0855 g·L-1,solid content was 6.60 g·L-1 and turbidity was 9.967 NTU.Average particle size of 80.9% particles was 1.170μm and average Zeta potential of 98.2% particles was -0.778 mV. Conclusion:This clarification process is stable and feasible with a good clarifying effect,and Yinma oral liquid system is stable after clarification.
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction process of Xiaoer Feike oral liquid and provide a reference for industrial production of this preparation. Method:Developing the antitussive,antiasthmatic and expectorant experiments,the best extraction method was optimized.Single factor tests was adopted to investigate effects of the amount of water,soaking time and extraction time on extraction process of volatile oil.With contents of ginsenoside Rb1 and hesperidin,dry extract yield as comprehensive evaluation index,orthogonal design was adopted to predict ethanol process conditions by taking ethanol volume,extraction time and times as factors. Result:Optimum extraction process of volatile oil were as follows:adding 8 folds of water,extracting for 8 h after being soaked 0.5 h;yield of volatile oil was 0.81 mL.The best conditions for ethanol extraction process were extracting thrice with 8 times of 75% ethanol,2 hours for per time.Extracting amounts of ginsenoside Rb1 and hesperidin were 1.59,2.84 mg·g-1,dry extract rate was 19.53%. Conclusion:These optimized technologies are stable and reliable for industrial production of Xiaoer Feike oral liquid.关键词：ginsenoside Rb1;hesperidin;volatile oil;antitussive test;expectorant test;antiasthmatic test;Xiaoer Feike oral liquid
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发布时间：2024-01-04 - 摘要：Objective:To analyze the volatile constituents of total lignans from Schisandrae Chinensis Fructus, and study its antitumor and anti-tobacco mosaic virus (TMV) activity. Method:The volatile constituents of total lignans from Schisandrae Chinensis Fructus were analyzed by GC-MS. The anti-TMV and anti-tumor effect were studied by half-leaf necrosis method and MTT method respectively. Result:The 58 compounds were detected from the total lignans from Schisandrae Chinensis Fructus, and 43 of them were identified, and the non-lignans accounted for 67.06% in all identified compounds. The compounds with higher content level included schizandrin (13.974%), carvopinone (8.064%), dehydroaromadendrene (6.063%), valerianol (5.416%), and gomisin A (4.732%). Their non-lignans components accounted for 67.06%. The anti-TMV and anti-tumor data showed certain inhibitory activity of total lignans from Schisandrae Chinensis Fructus on A549 cells proliferation, and good inhibitory activity on TMV. Conclusion:It is the first time to analyze low polar chemical components of total lignans from Schisandrae Chinensis Fructus by GC-MS, which is simple, fast, accurate and reliable. It provides a more efficient detection method for non-lignans of total lignans from Schisandrae Chinensis Fructus, and also provides a scientific foundation for the study of total lignans on their chemical and biological functions.关键词：Schisandrae Chinensis Fructus;total lignans;GC-MS;anti-TMV activity;anti-tumor activity
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发布时间：2024-01-04 - 摘要：Objective:To design the synthesis of high epigallocatechin gallate (EGCG) alkylated derivatives, analyze the structure-activity relationship and the pharmacological activity in inhibiting human hepatocellular carcinoma cell proliferation, in order to select the more stable and effective EGCG derivatives. Method:With EGCG and (CH3 CH2 O)2 SO2 as raw materials, a series of EGCG derivatives were ethylated. The effect of ethylated EGCG on the proliferation of human hepatocellular carcinoma cell SMMC-7721 and HepG2 were determined by 3-(4,5 dimethylthiazol-2-yl)-2,5 dimethyl-2H-tetrazolium bromide (MTT) method. With the cell density at 1×108/mL, the 3/4 of EGCG derivatives were diluted into four concentrations, with 60, 80, 100, 150 mg·L-1, and 30, 40, 50, 60 mg·L-1 for SMMC-7721 cells, and 60, 80, 100, 150 mg·L-1, and 20, 30, 40, 50 mg·L-1 for HepG2 cells, both were drug groups. A cell control group was also set up. Each concentration group has four complex wells, and the cells were continuously cultured for 24 h after addition of drugs. Result:We synthesized four EGCG derivatives. Their structures were identified by 1H-NMR,13C-NMR,2DNMR, mass spectrometry and other methods, all of them were novel compounds. Ethylated EGCG product 3 and 4 can inhibit proliferation of hepatocellular carcinoma cell SMMC-7721 and HepG2. Conclusion:Ethylated EGCG product 3 and 4 have inhibition effect on hepatocellular carcinoma cell SMMC-7721 and HepG2, and 4 has a much stronger effect than EGCG.关键词：epigallocatechin gallate;ethylated epigallocatechin gallate;antineoplastic activity
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发布时间：2024-01-04 - 摘要：Objective:To develop near-infrared spectroscopy (NIR) quantitative analysis model for water-soluble extract in Prunellae Spica. Method:The extract content of Prunellae Spica in 180 batches was determined by hot dipping method. NIRS spectrograms were measured, and the quantitative analysis model for extract was established by TQ8.0 software. Result:The correlation cross-validation coefficients (R2), root-mean-square error of calibration (RMSEC) and root-mean-square error of prediction (RMSEP) of the quantitative calibration model for water extract were 0.9814, 0.347 and 0.378 respectively, and the test value of NIR prediction values and the reference values of the validation set was 0.653, bilateral P=0.517>0.05, implying the difference was not significant. Conclusion:The method is steady, accurate and reliable to predict the water extracts content of Prunellae Spica by NIR.关键词：Prunellae Spica;water extracts;NIR spectroscopy
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发布时间：2024-01-04 - 摘要：Objective:To establish a method of fingerprint analysis on Polygoni Multiflori Radix formula granules by UPLC, identify the main chemical compounds, and provide a scientific method for rapid quality evaluation of Polygoni Multiflori Radix formula granules. Method:Ultra Performance Liquid Chromatography (UPLC) was used on the Agilent Eclipse Plus C18 RRHD (2.1 mm×100 mm,1.8μm), with acetonitrile-water as the mobile phase for gradient elution. The detective wavelength was set at 254 nm;the flow rate was 0.4 mL·min-1 and the column temperature was maintained at 30℃.Meanwhile, time of flight mass spectroscopy was used to analyze the main chemical compounds in fingerprints. Result:UPLC fingerprints were developed for Polygoni Multiflori Radix formula granules. 12 common peaks were determined,and 5 of them were identified. The similarity was higher than 0.9 between the fingerprints of the different samples of Polygoni Multiflori Radix formula granules and the control fingerprints. Conclusion:The method with good reproducibility is simple, rapid and accurate, which can be used to rapidly and effectively evaluate the quality of Polygoni Multiflori Radix formula granules.关键词：Polygoni Multiflori Radix formula granules;UPLC;fingerprints;compounds identification
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发布时间：2024-01-04 - 摘要：Objective:To study the effective fraction and compounds from Siraitiae Fructus on inhibition of Escherichia coli biofilms. Method:The E. coli bacterial biofilms (BBF) was used as the model to screen the effective fraction of Siraitiae Fructus. The chemical compounds were isolated from effective fraction by column chromatography to identify their structures and study their effect on E. coli bacterial biofilms. Result:95% ethanol elution fraction was the effective fraction, and 3 compounds were obtained from it, including palmitic acid(1), ring-(leucine-isoleucine acid)(2) and daucostero(3) respectively. Under the concentration of 1000 mg·L-1, the inhibition rates of compound 1, 2, 3 were 51.47%, 76.34% and 67.01% on E. coli bacterial biofilms. Conclusion:Compound 3 is isolated from Siraitiae Fructus for the first time.关键词：Siraitiae Fructus;Escherichia coli;bacterial biofilm;daucostero
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发布时间：2024-01-04 - 摘要：Objective:Establishment of HPLC-DAD determination of 4 major pharmacodynamic constituents of the mixture of coffee acid, paeoniflorin,baicalin,buddleoside, and the content of the main components of Angeitides mixture I. Method:The Agilent extend-C18 column (4.6 mm×150 mm, 5μm) was used, the mobile phase was acetonitrile-0.5% aqueous solution of phosphoric acid with gradient elution at a flow rate of 1 mL·min-1.The column temperature was kept at 25℃ and the detection wavelength of the coffee acid, paeoniflorin,baicalin,buddleoside was 323,230,280,334 nm. Result:The calibration curves of caffeic acid,paeoniflorin,baicalinand buddleoside were linear in the range of 0.0448-0.224(r=0.9999),0.5328-2.664μg(r=0.9998),0.6784-3.392μg(r=0.9999),0.1000-0.5000μg(r=0.9998) and the average recoveries were 100.62%(RSD 1.7%),99.36%(RSD 0.9%),101.50%(RSD 1.4%),99.05%(RSD 1.1%), respectively. Conclusion:The HPLC method is simple, fast, reliable and reproducible,providing a reference for quality control Angeitides mixture I.关键词：Angeitides mixture I;caffeic acid;paeoniflorin;baicalin;buddleoside
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发布时间：2024-01-04 - 摘要：Objective:To establish a measurement method for HPLC fingerprints of Shiduqing capsule, to evaluate the quality stability of Shiduqing capsule, and provide a more scientific and effective method for the quality control of Shiduqing capsule. Method:The fingerprint of Shiduqing capsule was performed on Agilent XDB-C18 column (4.6 mm×250 mm,5μm) with acetonitrile-0.1% phosphoric acid as gradient mobile phase. The flow rate was set at 1.0 mL·min-1. The detection wavelength was set at 220 nm and the column temperature was maintained at 30℃. Traditional Chinese medicine (TCM) chromatographic fingerprint similarity evaluation system (2004AEdition) by State Pharmacopeia Committee of China was used to evaluate the similarity of 10 batches of Shiduqing capsules. Result:The HPLC fingerprint of the Shiduqing capsules was established. Taking baicalin as the reference peak, 21 common peaks were found from the compounds. RSD of the relative retention time for each common chromatographic peak was less than 1% and the RSD of relative peak area was less than 4%. The method has good precision, repeatability and stability. With this method to determine the HPLC fingerprints of 10 batches of Shiduqing capsules, the similarity value was more than 0.95 for all of them. Conclusion:The quality of 10 batches of Shiduqing capsules is stable. This method is simple and easy to apply, with good precision, reproducibility and stability, which could manifest the overall quality characteristics of the Shiduqing capsules, and provide a scientific basis for the production and quality control of Shiduqing capsules.关键词：Shiduqing capsules;HPLC;fingerprint;quality control
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the simultaneous determination of peimine, peiminine and iso-peimine in Wanbei Zhike pill by HPLC. Method:The analysis was carried out on a CAPCELL PAK C18 column, with column temperature of 35℃;the mobile phase was acetonitrile-0.1% triethylamine solution (70:30) with a flow rate of 1.0 mL·min-1;ELSD parameters:the drift tube temperature was set at 95℃ and the nitrogen flow rate was 2.2 mL·min-1. Result:Peimine, peiminine and iso-peimine had good linear relationship respectively at the range of 34.33-343.3, 30.17-301.7, 82.11-821.1 mg·L-1;the average recovery rate for peimine, peiminine and iso-peimine was 97.55%,97.99% and 98.42% with RSD of 1.3%,1.8% and 1.4% respectively. Conclusion:The method is simple, accurate, reliable, and can be used as a method for quality control in Wanbei Zhike pill.关键词：Wanbei Zhike pill;peimine;peiminine;iso-peimine;HPLC-ELSD
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the determination of quercetin, puerarin, and rutin in flavonoids of Qingping tablet, and provide a reference for the quality control of Qingping tablet. Method:The samples were extracted with ultrasonic extraction method. The Kromasil C18 column(4.6 mm×250 mm, 5μm) was used with a mobile phase of methyl alcohol-0.05% TFA for gradient elution. The flow rate was 1.0 mL·min-1,with column temperature of 30℃ and the detection wavelength was 247nm. Result:Puerarin, rutin and quercetin showed good linear relationship within the range of 0.0036-0.1140 g·L-1(r=0.9998), 0.0033-0.1050 g·L-1(r=0.9996) and 0.0032-0.1020 g·L-1(r=0.9997) respectively. The average recovery rate of three components was 99.60% with RSD 0.4%, 99.86% with RSD 0.3% and 99.94% with RSD 0.2% respectively. Conclusion:The method is sensitive, simple, repeatable and controllable.It is a better method for determination of flavonoids content in Qingping tablet.关键词：HPLC;Qingping tablet;quercetin;puerarin;rutin
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发布时间：2024-01-04 - 摘要：Objective:Preparating resolvability of chemical components of Cyathulae Radix and investigating water decoction and its chemical detached composition of Cyathulae Radix which impact on immune regulation. Method:We adopted the technology of hot water extraction, alcohol sedimentation and macroporous adsorptive resins to establish the split method of chemical components of Cyathulae Radix and four compositions obtained were fatty oils composition, polysaccharide composition, ketosteroid composition and 20% ethanol elution composition, repectively. We adopting the technology of high performance liquid chromatography to study their chemical property and the chemical composition was detached uncross in each composition. Investigating water decoction and its chemical detached composition of Cyathulae Radix which impact the carbon clearance index and the influence of immune organs in mice and in mice serum hemolysin antibody generatin on immune regulation. Result:The results indicated that establishing the detached method based on the chemical components of Cyathulae Radix was practicable, steady and good reproducibility. The characteristic of this method was holo-composition which was detached uncross in each. Cyathulae Radix had the certain effect of immunoenhancement. Conclusion:The effect of immunoenhancement of Cyathulae Radix which was based on polysaccharide and ketosteroid composition.关键词：Cyathulae Radix;chemical separation components;immune regulation effects
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发布时间：2024-01-04 - 摘要：Objective:To establish the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum. Method:HPLC was performed on ZORBAX SB -C18 column (4.6 mm×150 mm, 5μm), eluting in a gradient mode with the mobile phase of acetonitrile (A)-0.2%H3 PO4 as (B) at a flow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 360 nm. The injection volume was 10μL. Result:Eleven common characteristic peaks were identified as fingerprint peaks, the methodological study was consistent with the technical requirements. Conclusion:The established method is stable, reliable and highly accurate, and can provide reference for quality control of G.pentaphyllum.关键词：Gynostemma pentaphyllum;flavonoids;HPLC;fingerprint spectrum
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发布时间：2024-01-04 - 摘要：Objective:To investigate the inhibitory effect and mechanism of Danzhi Xiaoyao San(DZXYS) on human breast cancer cell line MCF-7 in nude mice. Method:MCF-7 cells were inoculated in nude mice armpit to establish the subcutaneous tumor model. Female mice were divided to four groups randomly:model group, high-dose DZXYS group (17.98 g·kg-1), middle-dose DZXYS group (8.99 g·kg-1), and low-dose DZXYS group (4.50 g·kg-1). Mice of high, middle and low-dose DZXYS groups were orally administrated with DZXYS and model groups with equivalent saline. The treatment course was 15 days. The tumor inhibition rate of tumor mass was observed. Tumor tissue morphology was observed by hematoxylin and eosin(HE) staining. Apoptosis of tumor tissue was observed by Tunel staining. Result:Compared with the model group, DZXYS was statistically significant in middle and high-dose group, and showed a notable tumor mass inhibition effect (P<0.05, P<0.01), improved tumor morphology, and increase in the number of cells in tumor tissues stained with Tunel and the tumor cell apoptosis rate (P<0.01). Conclusion:DZXYS can inhibit the growth of MCF-7 tumor tissue in nude mice, and its mechanism may be related to promoting tumor cell apoptosis.关键词：Danzhi Xiaoyao San;breast cancer;MCF-7 cell;apoptosis
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of water extruct from cortex of Berberi dictyophylla (BDCE) on diabetic retinopathy (DR) in spontaneous type 2 diabetic db/db mice. Method:Eighteen-week-old db/db mice were selected, with their thin db/m mice as normal controls. The db/db mice were randomly divided into 6 treatment groups:model control (db/db) group, high and middle and low dosage groups of BDCE (1.50, 0.75, 0.38 g·kg-1), calcium-dobesilate group (0.23 g·kg-1), berberine hydrochloride group (0.135 g·kg-1). During the two-month intragastric administration, all of the mice were weighed and measured for their fasting blood glucose level every two weeks, all the mice were put to death and their eyeballs were taken out at the end of the two-month treatment. Conventional hematoxylin and eosin(HE) staining was performed to observe pathologic change, retina stretched preparation was performed to observe microvascular changes and calculated endothelial/pericytescells (E/P) value. Result:Compared to db/m group, db/db group was significantly higher (P<0.01) in body mass, fasting blood glucose level (P<0.01) and E/P value (P<0.05) of retina microvascular, and showed capillary congestion, expansion and damage of retina. Compared to db/db group, all treatment groups were not significantly higher in body mass;berberine hydrochloride group and low dosage of BDCE group were significantly lower in fasting blood glucose level (P<0.01), high and middle dosage group of BDCE were significantly lower in blood glucose level (P<0.05), calcium-dobesilate group showed no significant change in fasting blood glucose level;significantly lower in E/P value (P<0.05) was obtained in high BDCE group, while all of other treatment groups showed no significant change in E/P value, and retinal damage were relieved in all BDCE-treated groups. Conclusion:Spontaneously diabetic db/db mice can be considered as an animal model for human DR. BDCE shows good preventive effects for DR, significantly decreases in blood glucose level, relieves spontaneous retinal microvascular damage in diabetic mice, and protects retinal capillaries while preventing the formation of new blood vessels.关键词：water extract from cortex Berberidis dictyophylla;spontaneous type 2 diabetic db/db mice;diabetic retinopathy;fasting blood glucose level;retinal morphology
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Qibai Pingfei (QBPF) capsule on chronic obstructive pulmonary disease (COPD) rats induced by lipopolysaccharides(LPS) and cigarette smoke, in order to determine its mechanism. Method:Normal rats of control group were bred for 4 weeks in normal circumstance before test and instilled intratracheally with normal saline once at day 1 and day 14.Rats of COPD group were exposed to cigarette smoke 2 h·d-1 for 4 weeks and intratracheally instilled with 200μg LPS once at day 1 and day 14.All COPD rats were divided randomly into five groups. Each group was orally administrated with QBPF granules 4.4,2.2,1.1 g·kg-1 and aminophylline 0.054 g·kg-1 respectively, while model group was administrated with solvent. After 4 weeks, lung function was recorded after administration, level of serum interleukin-1(IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6(IL-6), interferon-γ (IFN-γ) and lung tissue morphology were observed. Result:Compared with normal group, lung function indexes such as forced vital capacity (FVC), forced expiratory volume in 0.3 second (FEV 0.3), FEV 0.3/FVC, peak expiratory flow (PEF) decreased significantly(P<0.01);serum levels of IL-1β, and IL-6, IFN-γ, TNF-α significantly increased (P<0.01). Compared with model group, lung function indexes such as FVC, FEV 0.3, FEV 0.3/FVC, PEF rose significantly in the middle and high dose of QBPF group and aminophylline group (P<0.05, P<0.01). Compared with model group, QBPF granules group showed significant decrease in Serum levels of IL-1β, and IL-6, IFN-γ, TNF-α but increasein level of IL-10(P<0.05, P<0.01). Conclusion:QBPF granules can improve lung function, reduce the pathological changes of lung tissue. Its inflammatory reduction factors may be an important mechanism for treatment of chronic obstructive pulmonary disease.关键词：Qibai Pingfei granules;chronicobstructive pulmonary disease;interleukin-1;tumor necrosis factor-α;interleukin-6;interleukin-10;interferon-γ
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发布时间：2024-01-04 - 摘要：Objective:To preliminarily evaluate the effect of 17 main components from Guizhi Fuling capsule on human leiomyoma cell proliferation and dysmenorrhea. Method:Human leiomyome cells were grown in 96-well plates and treated with 17 main components (25 mg·L-1) for 48 h. There were 6 complex wells for each group. Then Hoechst 33342/Propidium iodide(PI) double staining method was used to analyze the effect of the 17 main components on human leiomyome cell, and the anti-dysmenorrhea bioactivity of the 17 components was evaluated by the model Prostag landin F2α(PGF2α)-induced contraction of isolated mouse uterine, with 10 parallel tests for each group. Result:Compared with control group, quercetin, cinnamyl aldehyde, paeonol, pachymic acid, dehydrotumulosic acid, albiflorin and paeoniflorin inhibited human leiomyoma cell proliferation, with significant difference (P<0.05, P<0.01). Compared with control group, gallic acid, paeoniflorin, paeonol, laetrile, benzoylpaeoniflorin and 1, 2, 3, 4, 6-O-pentagalloylglucose significantly inhibited contraction of isolated mouse uterine (P<0.01). Conclusion:Quercetin, cinnamyl aldehyde, paeonol, pachymic acid, dehydrotumulosic acid, albiflorin and paeoniflorin in Guizhi Fuling capsule may be active ingredients for treating hysteromyoma, while gallic acid, paeoniflorin, paeonol, laetrile, benzoylpaeoniflorin and 1, 2, 3, 4, 6-O-pentagalloylglucose in Guizhi Fuling capsule may be active ingredients for treating primary dysmenorrhea.关键词：Guizhi Fuling Capsule;hysteromyoma;cell proliferation;dysmenorrhea;uterine contraction
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Lepidium meyenii lipid-soluble extract on the decline of the learning and memory ability of sleep deprived mice, and analyze the lipid-soluble extract. Method:Totally 48 male Kunming mice were randomly divided into 4 groups, large platform control group, sleep deprivation group, low dose group of L. meyenii lipid-soluble extract (60 mg·kg-1) and high dose group of L. meyenii lipid-soluble extract (200 mg·kg-1), they were administrated by lavage for 16 days. On the 13th day, a small platform was used to establish sleep deprivation (SD) model in mice. The ability of learning and memory was tested by channel maze after SD 24, 48, 72 h, and pathological changes of brains were observed after SD 72 h. The levels of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in cerebrum were measured. The components of L. meyenii lipid-soluble extract were analyzed by gas chromatography-mass spectrometer (GC-MS) and high performance liquid chromatography (HPLC). Result:Compared with a large platform, the latency to swim out of the water maze and the error times of blind ends increased significantly in the sleep deprivation group (P<0.05). Supplementation with L. meyenii lipid-soluble extract (200 mg·kg-1) could reduce the latency to swim out of the water maze significantly (P<0.05) and the error times of blind ends. Moreover, the levels of MDA reduced significantly (P<0.01), the activities of SOD and GSH-Px in cerebrum returned to normal. And the damage of hippocampal structures decreased. Conclusion:Supplementation with L. meyenii lipid-soluble extract which contained macamides can improve learning and memory ability and decrease the damage of hippocampal structures in sleep deprived mice, and the mechanism may be correlated with the inhibition of lipid peroxidation.关键词：Lepidium meyenii;lipid-soluble extract;sleep deprivation;learning memory;water maze;antioxidant
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发布时间：2024-01-04 - 摘要：Objective:To compare the protective effect of 18α-and 18β-glycyrrhizic acid on acute liver injury in mice, and screen out their optimal combination proportion. Method:The mice were randomly divided into normal group, model group, high dose group (60 mg·kg-1), middle dose group (30 mg·kg-1), low dose group (15 mg·kg-1) of 18α-and 18β-glycyrrhizic acid. With the drug dose of combined medication groups of 60 mg·kg-1, combined medication 1 groups (18α-glycyrrhizic acid-18β-glycyrrhizic acid 1:3), combined medication 2 groups (18α-glycyrrhizic acid-18β-glycyrrhizic acid 2:3), combined medication 3 groups (18α-glycyrrhizic acid-18β-glycyrrhizic acid 1:1). Before the modeling, the drugs were injected once everyday for 7 days, the same volume of saline was given to normal group and model group. At 1 h after the last administration, the treated groups and model group were ip given 0.1%CCl4 peanut oil (20 mL·kg-1) to establish mouse model of acute liver injury induced by CCl4 .The samples of blood and liver from high does group, middle dose group, low dose group and three combination proportion groups of 18α-and 18β-glycyrrhizic acid were analyzed, and level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Result:Compared with the normal group, the serum levels of AST, liver homogenate levels of AST, ALT, MDA in model group were remarkably higher (P<0.01),levels of SOD was remarkably lower. Compared with the model group, 18α-and 18β-glycyrrhizic acid remarkably reduced the serum levels of AST, reduced liver homogenate levels of AST, ALT and MDA, and increased liver homogenate content of SOD. 18β-glycyrrhizic acid had a better effect on liver homogenate levels of AST,ALT than 18α-glycyrrhizic acid, and showed no significant difference in other indexes compared with 18β-glycyrrhizic acid. The optimal combination proportion of 18α-and 18β-glycyrrhizicacid is 2:3, with a better effect on liver homogenate levels of AST, MDA than simple 18α-or 18β-glycyrrhizic acid. Conclusion:18α-and 18β-glycyrrhizic acid can protect acute liver injury in mice. The latter had a better protective effect than the former. Their optimal combination proportion is 2:3, with a better effect on liver homogenate levels of AST, MDA than simple 18α-or 18β-glycyrrhizic acid.关键词：18α-glycyrrhizic acid;18β-glycyrrhizic acid;acute liver injury;protective effects;combination proportion
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发布时间：2024-01-04 - 摘要：Objective:To study the preventive and therapeutic effects of the combination of Polygoni Cuspidati Rhizoma et Radix and Cinnamomi Ramulus(PC) on hyperuricemia, and its effect on urate-anion transporter 1(URAT1), organic anion transporters 3(OAT3) and ATP-bindingcassette subfamily G member 2(ABCG2) in rats. Method:Eighty four male SD rats were randomly divided into 7 groups:the normal group, the model group, the benzbromarone (10 mg·kg-1) group, the Tongfengding capsule (160 mg·kg-1) group and PC compatibility low-, medium-and high-dose (3.5,7,14 g·kg-1) groups, with 10 rats in each group. The hyperuricemia model in rats was established through ip injection of potassium oxonate (200 mg·kg-1), ig administration of hypoxanthine (50 mg·kg-1) and ethambutol (250 mg·kg-1). Since the fourth day, all the rats were intragastrically administered with drugs for 2 weeks, once a day, in order to determine their serum uric acid (SUA), creatinine (Cr), blood urea nitrogen (BUN), glutamic-pyruvic transaminase (GPT), as well as urinary uric acid (UUA) levels. The RT-PCR method was used to measure the mRNA levels of renal URAT1, OAT3 and intestinal ABCG2 in rats. Renal URAT1 and OAT3 protein expressions in rats were determined by Western blotting method. Intestinal ABCG2 protein expression in rats was assayed by immunohistochemistry method. Result:Compared to the normal group, SUA levels of the model group increased significantly and excretion of UUA decreased, with high expressions of renal URAT1 mRNA and protein, low expressions of renal OAT3 and intestinal ABCG2 mRNA and protein expression. The results between the normal group and the model group showed significant statistical differences (P<0.01). Compared to the model group,PC medium-dose and high-dose groups showed significantly reduction in SUA and BUN levels, and increase in 24 h excretion of uric acid. PC medium-dose and high-dose groups showed inhibition in renal URAT1 mRNA and protein levels, and increase in renal OAT3 and intestinal ABCG2 mRNA and protein levels. The results between the two groups showed significant statistical differences (P<0.05, P<0.01). Conclusion:PC has an obvious effect of anti-hyperuricemia. It may down-regulate the expression of renal URAT1 and up-regulate the expressions of renal OAT3 and intestinal ABCG2 in rats to reduce re-absorption of uric acid and increase the excretion of uric acid.关键词：combination of Polyonum Cuspidatum and Ramulus Cinnamomi;hyperuricemia;urate-anion transporter 1;organic anion transporters 3;ATP-bindingcassette subfamily G member 2
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the protective effects of (-)-epigallocatechin gallate (EGCG) combined with procyanidins B2 (PC-B2 ) on liver cell damage caused by aflatoxin B1 (AFB1 ). Method:L-02 cells were selected in an experimental study in vitro and divided into 6 groups:control group; solvent control group; AFB1 group; EGCG+AFB1 group; PC-B2 +AFB1 group; EGCG+PC-B2 +AFB1 group. The cells survival was determined by MTT; DNA damage was detected by single cell gel electrophoresis (SCGE); the flow cytometry method was used to detect cell apoptosis; and the expressions of protein about apoptosis signaling pathways (Bcl-2, Bax, Caspase-3, Caspase-9, P53) were detected with Western blot method. Result:EGCG and PC-B2 can reduce liver cell growth inhibition caused by AFB1 from 61.12% to 42.18% and 46.72%; EGCG combined with PC-B2 made a better effect. SCGE results showed that either EGCG or PC-B2 can reduce liver cell DNA damage caused by AFB1 . Simple or combined administration of EGCG and PC-B2 can decrease the early apoptosis rate of L-02 cells induced by AFB1 (P<0.05). Western blot experiments showed that after treatment with AFB1 , EGCG or (and) PC-B2 treatment can reduce the expression of Caspase-3 and Caspase-9 significantly (P<0.01), and improve the ratio of Bcl-2 to Bax, but with no obvious expression of P53. Conclusion:EGCG and PC-B2 can protect the human normal liver cells from the damage induced by AFB1 reducing L-02 cell DNA damage and inhibiting the apoptosis, and the mechanism may be correlated with the down-regulation of Caspase-3 and Caspase-9 expressions and the increase in Bcl-2/Bax.关键词：epigallocatechin gallate;procyanidine B2;aflatoxin B1;DNA damage;apoptosis
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发布时间：2024-01-04 - 摘要：Objective:To study the effect and mechanism of Kangxianling docotion on renal fibrosis in mice with 5/6 nephrectomy. Method:The 60 C57 mice were randomly divided into sham operation group (n=10) and operation group (n=50). The patients in operation group underwent 5/6 nephrectomy. After two weeks, the patients in operation group were randomly divided into model group, Kangxianling low, medium and high dose groups (0.1, 0.2,0.4 mg·kg-1)and rapamycin group(0.16μg·kg-1), n=10 in each group. In each group were given 0.5 mL normal saline, rapamycin and Kangxianling were respectively given by gavage for the groups after two weeks. Mice were sacrificed after 8 weeks of treatment. 1 day before they were sacrificed, 24 h urine was collected to detect 24 h protein, and orbital blood test was taken for serum creatinine, blood urea nitrogen. The remnant kidney was taken to detect α-SMA expression by immunofluorescence method. The type Ⅰ and Ⅲ collagen protein expression in renal tissues was detected by Western blot assay. Result:Compared with the sham group, 24-hour urinary protein excretion, serum creatinine, blood urea nitrogen, α-SMA, type Ⅰ and Ⅲ collagen protein expression were significantly increased in the model group (P<0.01).Compared with the model group, 24-hour urinary protein excretion, serum creatinine, blood urea nitrogen, α-SMA, type Ⅰ and type Ⅲ collagen expression were decreased in each treatment group(P<0.05). Conclusion:Kangxianling docolion can decrease 24 h urinary protein excretion in mice, improve renal function and delay the occurrence of renal fibrosis, and its mechanism may be related to lowering α-SMA expression and inhibiting type Ⅰ and type Ⅲ collagenl expression in renal tissues.关键词：Kangxianling docotion;renal fibrosis;smooth muscle actin;type Ⅰ collagen;type Ⅲ collagen
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of compound Tripterygii Radix et Rhizoma on spermatocytes apoptosis in mice and their expression of apoptosis-related Bax, Bcl-2, and Caspase-3. Method:In vitro culture system for mice spermatocytes was established. The mice were divided into blank group, triptolide 3.5 mg·L-1 group, triptolide 3.5 mg·L-1+catalpol 3 mg·L-1 group, triptolide 3.5 mg·L-1+panax notoginseng saponins 12 mg·L-1 group, triptolide 3.5 mg·L-1+oxalate 1.5 mg·L-1 group, triptolide 3.5 mg·L-1+sinomenine 0.75 mg·L-1 group. Flow cytometry was used to detect the spermatocytes apoptosis rate after 24 h drug treatment, Western blot assay was done to determine the protein expression of Bax, Bcl-2, and Caspase-3 in spermatocytes. Result:Catalpol and Panax notoginseng saponions could reduce the induction effect of triptolide on apoptosis in mouse spermatocytes, with significant differences(P<0.05) compared with triptolide group, but there was no significant difference between ammonium oxalate, Sinomenine group and triptolide group. Triptolide induced increase in Bax, Caspase-3 protein expression, decrease in Bcl-2 protein expression, with significant difference with blank group (P<0.01). After combination of triptolide with catalpol and panax notoginseng saponins, Bax, Caspase-3 protein expression was reduced, while Bcl-2 protein expression was increased, with significant difference compared with triptolide group (P<0.05). The combination of triptolide with ammonium oxalate and Sinomenine did not make significant difference. Conclusion:Triptolide can cause spermatocytes apoptosis through Bax/Bcl-2, and Caspase-3 system. The other drug components in compound in Tripterygii Radix et Rhizoma can inhibited apoptosis in spermatocytes to varying degrees, and reduce its reproductive toxicity.关键词：Tripterygii Radix et Rhizoma;spermatocytes;apoptosis;apoptotic proteins
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发布时间：2024-01-04 - 摘要：Objective:To determine the total triterpenes and total sterol content in petroleum ether extract, EtOAc extract, and MeOH extract of the fruiting body of Fomitopsis officinali, and study the anti-tumor activity of three kinds of crude extracts and triterpene monomer compounds isolated from EtOAc extract, and explore the relationship between its anti-tumor activity and the content of total triterpenes and total sterol content. Method:Total terpene and sterol content in three crude extracts was determined by spectrophotometric method. Based on the in vivo anti-tumor experiment in H22 tumor-burdened transplantation mice models, the petroleum ether extract, EtOAc extract, and MeOH extract of the fruiting body as well as triterpene monomer compounds were observed and evaluated for anti-tumor activity. Result:The total triterpenes and total sterol content is the highest in EtOAc extract, followed by petroleum ether extract. Five monomer compounds were isolated and obtained from EtOAc extract:dehydroeburiconic acid, 3-keto-dehydrosulphurenic acid, dehydroeburicoic acid, fomlactone A and fomlactone C. In the in vivo experiment, EtOAc fraction has the best anti-tumor activity, and when its concentration reached 1000 mg·kg-1,its anti-tumor rate was 64.65%, closed to the positive drug 5-fluorouracil (anti-tumor rate of 59.98%), and the immune organ indexes also had some changes. The anti-tumor rate of compound 2(3-keto-dehydrosulfurenic acid) was 75.13%, closed to the positive drug CTX (anti-tumor rate of 81.76%), highter than positive drug 5-Fu. The other four kinds of monomer compounds all had a certain effect on the tumor. Conclusion:EtOAc extract and five monomer compounds can effectively inhibit tumor growth, and can improve the body's immune system. These five monomer compounds are the effective anti-tumor ingredients of the fruiting body of F. officinali, and the active anti-tumor ingredient may be triterpene compounds in F. officinalis.关键词：Fomitopsis officinalis;total triterpenes;anti-tumor activity in vivo;anti-tumor rate
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发布时间：2024-01-04 - 摘要：Objective:To investigate the role of Kiwi essence in sensibilization of chemotherapeutics for allotransplantation tumors in mice with lung adenocarcinoma, and preliminarily discuss the related mechanism thereof. Method:Transplantation tumor models were established in mice with lung adenocarcinoma. 40 C57BL/6J mice bearing Lewis cells were randomized into five groups, n=8 in each group:normal group (normal saline), K. essence group (K. essence 180 mg·kg-1), chemotherapy group (cisplatin and gemcitabine), 60 mg·kg-1 K. essence combined with chemotherapy group, 180 mg·kg-1 K. essence combined with chemotherapy group. After inoculated with adenocarcinoma cells for 4 days, corresponding drugs were given for intervention. Mice were sacrificed 20 days after tumor inoculation, and the subcutaneous tumors were stripped for histological examination. The expressions of excision repair cross-complementation groupl(ERCC1) and ribonucleotidereductase subunit M1(RRM1) for chemotherapy-related drug resistance genes were detected by Western blot assay and RT-PCR. Result:Compared with the normal group, the tumor growth of various drug intervention groups was inhibited significantly (P<0.05,P<0.01), and the anti-tumor rate was 14.95%, 41.77%, 44.21%, 59.68% respectively in various groups. Expression of ERCC1 gene was highest in normal group, and significantly decreased in various K. essence essence groups, with statistically significant difference (P<0.01). Expression of ERCC1 in K. essence groups and K. essence high dose+chemotherapy group was significantly lower than other groups(P<0.01)。ERCC1 mRNA expression and ERCC1 proteins expression were in consistency. Expression of RRM1 gene was highest in normal group, nd significantly decreased in various K. essence groups, with statistically significant difference (P<0.01,P<0.05). Expression of RRM1 in K. essence groups and K. essence high dose+chemotherapy group was significantly lower than other groups. Conclusion:K. essence could increase the ability of chemotherapeutics to inhibit tumor growth and transfer by reducing ERCC1 expression and increasing RRM1 expression.关键词：lung neoplasms;adenocarcinoma;excision repair cross-complementation groupl;ribonucleotidereductase subunit M1;Kiwi essence
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of Danggui Beimu Kushen Wan medicated serum on the inhibition of gastric cancer cells SGC-7901and its antineoplastic mechanisms. Method:The 120 Wistar rats were divided into Danggui Beimu Kushen Wan high dose group, middle dose group and low dose group (0.3, 0.2, 0.1 g·kg-1) and normal group, n=30 in each group, ig 2 times/d, continuous for 7 days to prepare drug serum. The rats whose gastric cancer cells SGC-7901 were treated by blank serum were used as blank group. In the experiment groups, SGC-7901 cells were treated by high dose, middle dose and low dose Danggui Beimu Kushen Wan medicated serum, and then RT-qPCR technique was used to detect the expression level of mRNA of cyclooxygenase-2(COX-2), Bcl-associated X protein(Bax), and phosphatase and tensin homolog deleted on chromosome ten(PTEN). Immunocytochemical technique was used to test the protein expression level of COX-2, Bax, and PTEN. Result:Compared with the normal group, high dose group decreased the expression of COX-2 mRNA in SGC-7901 cell by 68%, and increased the expression of Bax and PTEN mRNA by 99.7% and 85.6% respectively (P<0.05). Drug serum can reduce the positive expression rate of COX-2 for SGC-7901 cells, increase its average gray value. increase the positive expression rate of Bax and PTEN, rate and reduce the average gray value (P<0.05). Conclusion:Danggui Beimu Kushen Wan medicated serum could inhibit the expressions of Bax, PTEN and COX-2 at mRNA and protein level in SGC-7901 gastric cancer cells.关键词：Danggui Beimu Kushen Wan medicated serum;gastric cancer;SGC-7901;cyclooxygenase-2;Bcl-associated X protein;phosphatase and tensin homolog deleted on chromosome ten
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Rubi Fructus's different extract parts on the memory disorders induced by scopolamine, Sodium nitrite and 40% Ethanol in rats, explore the effective extract parts of Rubi Fructus for the 3 memory disorder models, and provide the experimental evidence for research of mechanism of Rubi Fructus in improving the memory ability. Method:Intragastric 130 Kunming mice were randomly divided into 13 groups, n=10 in each group, including blank group, model group, positive group, Rubi Fructus whole prescription high dose group and low dose group, chloroform extract high dose group and low dose group, n-butyl alcohol extract high dose group and low dose group, ethyl acetate extract high dose group and low dose group, aqueous extract high dose group and low dose group. Administration groups respectively received ig high dose and low dose (24, 12 g·kg-1) Rubi Fructus whole prescription, chloroform extract, n-butyl alcohol extract, ethyl acetate extract, and aqueous extract. The positive group received ginkgo leaves preparation 7.6 mg·kg-1. The blank group and the model group received same-volume-gavage of saline, continuous for 12 days. After 7 days, all mice except those in the blank group were intraperitoneally injected with corresponding 1 mg·kg-1 dose 30 min before test for the scopolamine-induced models, intraperitoneally injected with corresponding 120 mg·kg-1 after training for the sodium nitrite-induced models, and received intragastric administration of 0.1 mL·kg-1 30 min before test for 40% ethanol-induced models, to explore the navigation ability and space exploration ability in the Morris water maze. Result:Compared with the normal group, escape latency of three memory disorders were significantly prolonged, and the number of times for passing the platform was significantly reduced (P<0.05). Compared with the model group, Rubi Fructus whole prescription, chloroform extract, ethyl acetate extract, n-butanol extract, and water extract can shorten the escape latency of three memory disorders and enhance the number of times for passing the platform (P<0.05). Conclusion:Rubi Fructus's water extract high dose group has the best effect for improving the memory disorders induced by scopolamine and sodium nitrite. Rubi Fructus whole prescription high dose group has the best effect for improving memory disorders induced by 40% ethanol in mice.关键词：Rubi Fructus;learning and memory;scopolamine;sodium nitrite;40%ethanol
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发布时间：2024-01-04 - 摘要：Objective:To study effects of Zishen Qinggan formulaon on blood glucose, glycosylated hemoglobinAlc (HbAlc), C-peptide (C-P), glucagon-like peptide (GLP-1) and α-glucosidase activity in Goto-Kakizaki (GK) rats. Method:Rats with fasting blood glucose (FBG)>11.1 mmol·L-1were selected and randomly divided into the following groups:diabetic model group, Zishen Qinggan formula low dose group (ig, 300 mg·kg-1·d-1), middle dose group (ig, 600 mg·kg-1·d-1), and high dose group (ig, 1200 mg·kg-1·d-1), and metformin group (ig, 85 mg·kg-1·d-1). After 28 d of continuous medication, fasting blood glucose (FBG) was detected and oral glucose tolerance test (OGTT) was done, then HbAlc, C-P, GLP-1 levels and α-glucosidase activity were detected. Result:Compared with the normal group, FBG and HbAlc levels in model group were significantly higher (P<0.01). Compared with the model group, FBG, HbAlc levels and the blood glucose level after 120 min of glucose tolerance were significantly reduced in Zishen Qinggan formula groups (P<0.05, P<0.01), levels of C-P, and GLP-1 were significantly increased (P<0.05, P<0.01), insulin resistance index was reduced (P<0.05), and α-glucosidase activity was inhibited. Conclusion:Zishen Qinggan formula can effectively reduce blood glucose and improve insulin resistance in GK rats, and the action mechanism may be associated with increasing GLP-1 content and inhibiting α-glucosidase activity.关键词：Zishen Qinggan formula;type 2 diabetes mellitus;insulin resistance
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发布时间：2024-01-04 - 摘要：Objective:To investigate the clinical effect of Tangmaikang particles in combination with intensive insulin therapy in the treatment of type 2 diabetes mellitus (T2DM) and its effects on blood lipids and endothelin-1(ET-1). Method:The 124 cases of T2DM meeting the inclusion criteria in people's hospital of Binzhou were randomly divided into control group and observation group equally. Besides conventional treatments, the control group was also treated with intensive insulin therapy, while the observation group was also treated with Tangmaikang particles combined with intensive insulin therapy. Then, the curative efficacy, glucose-related indicators, level of blood lipids, ET-1 and incidence of hypoglycemia were compared between two groups. Result:After treatment, the effective rate in the observation group was 83.9%, significantly higher than 67.7% in the control group (P<0.05). In comparison with the control group after the treatment, glucose-related indicators in observation group were as follows:fasting plasma glucose (FPG) (7.2±0.4)VS(7.9±0.5)mmol·L-1, 2 h plasma glucose (2 hPG)(8.5±0.5)VS(9.4±0.6) mmol·L-1, glycosylated hemoglobin A1c(HbA1c)(7.0%±0.4%)VS(7.6%±0.5%). The levels were respectively significantly lower in the observation group (all P<0.05). As to blood lipids, level of total cholesterol(TC) (4.36±0.82)VS(5.21±0.94)mmol·L-1, triglyceride(TG)(1.93±0.68)VS(2.20±0.53)mmol·L-1, low density lipoprotein cholesterol(LDL-C)(3.10±0.54)VS(3.90±0.64)mmol·L-1, respectively statistically lower in the observation group. And level of high density lipoprotein cholesterol(HDL-C)(1.59±0.35)VS (1.29±0.32)mmol·L-1was statistically higher in the observation group than that in the control group after the treatment (all P<0.05). And level of ET-1(70.2±7.4)VS(75.6±8.3) ng·L-1 in the observation group was found statistically lower than that in the control group after the treatment (P<0.05). During the treatment, the incidence of hypoglycemia was 8.1% in the observation group and 4.8% in the control group, with no significant difference. Conclusion:Tangmaikang particles combined with intensive insulin therapy is effective for T2DM, which can significantly increase curative efficacy, improve blood glucose and blood lipids indicators and lower the level of ET-1, in addition, it would not increase the incidence of hypoglycemia, thus it is worthy for promotion in clinical use.关键词：Tangmaikang particles;intensive insulin therapy;type 2 diabetes mellitus;endothelin-1;hypoglycemia
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发布时间：2024-01-04 - 摘要：Objective:To observe the clinical effect and mechanism of Yiqi Ziyin Huoxue Tongluo prescription (referred to as Chinese medicine compound) in treating atherosclerosis. Method:One hundred and fifty patients which meet the diagnosis standard were randomly divided into atorvastatin group (30 cases), Chinese medicine compound low (40 cases), middle (40 cases) and high dose groups (40 cases). After treating for 0, 4, 8 weeks, the changes of intima media thickness (IMT), interleukin-6(IL-6), tumor necrosis factor-α (TNF-α), and matrix metalloproteinase-9(MMP-9) were compared among the groups. Result:Compared to atorvastatin group, IMT, IL-6, TNF-α, MMP-9 in Chinese medicine compound low dose group were significantly increased (P<0.05), however, there was no significant difference in Chinese medicine compound middle and high dose groups;IMT, IL-6, TNF-α, MMP-9 showed no significant difference within Chinese medicine compound low dose group, but showed significant difference within Chinese medicine compound middle and high dose groups (P<0.05). Conclusion:Chinese medicine compound can prevent and treat atherosclerosis, whose mechanism may be related to anti-inflammatory, and its dose-effect relationship and time-effect relationship are positively correlated respectively.关键词：Yiqi Ziyin;Huoxue Tongluo;interleukin-6;tumor necrosis factor-α;matrix metalloproteinase-9;atherosclerosis
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发布时间：2024-01-04 - 摘要：Objective:To observe clinical efficacy of Hanbiting tablet as an additional therapy for rheumatoid arthritis (RA) patients with cold dampness arthralgia spasm syndrome (CDASS) and its influence on levels of interleukin-17(IL-17) and prostaglandin E2 (PGE2 ) in synovial fluid and serum. Method:One hundred and fourteen RA patients of CDASS were randomly divided into control group and treatment group (57 cases each) by random number table. Two groups were given non-drug treatment on the basis of the Diagnosis and Treatment Guidelines of RA. Patients of control group were given methotrexate tablets (10 mg/time,1 time/week) and emulgel tablets (75 mg/time, qd, oral administration after dinner). Besides the therapy of control group, patients in treatment group were additionally treated with Hanbiting tablets with oral administration (3 tablets/time, tid). The course of treatment lasted for 12 weeks for both groups. Scores of traditional Chinese medicine clinical symptoms were compared to evaluated quality of life of patients in two groups by using the Chinese Version of Health Survey Questionnaire Shot Form (SF-36) before and after treatment. The clinical efficacy of both groups were analyzed after treatment, and levels of IL-17 and PGE2 in synovial fluid and serum were detected before and after treatment. Result:Scores of traditional Chinese medicine clinical symptoms in treatment group were all evidently lower than these of control group after treatment (P<0.01). The clinical effective rate of control group was 96.49%, which was superior to 82.46% of treatment group with statistical difference (P<0.05). The scores of SF-36 of treatment group were obviously higher than these of control group after treatment (P<0.01). The levels of IL-17 and PGE2 in the synovial fluid and serum in treatment group were obviously lower than control group with statistical difference (P<0.01). Conclusion:Hanbiting tablet, as an additional therapy for RA patients with CDASS, could remarkably relieve clinical symptoms and improve the quality of life and clinical efficacy. And its mechanism may be related with reducing levels of IL-17 and PGE2 in synovial fluid and serum.关键词：Hanbiting tablet;rheumatoid arthritis;cold dampness arthralgia spasm syndrome;interleukin-17;prostaglandin E2
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发布时间：2024-01-04 - 摘要：Objective:To discuss the curative effect of Jiawei Yigong powder in treating diabetes gastroparesis(DGP) and its influence on plasma motilin and gastrin. Method:Ninety four patients were randomly divided into control group (46 cases) and observation group (48 cases). Patients in control group took mosapride citrate tablets, 5 mg/time, 3 times/day, at 30 min before meal;Sulfur acid capsules, 0.6 g/time, 1 time/day, at 30 min before breakfast. Besides the therapy of control group, patients in observation group were additionally treated with Jiawei Yigong powder, 1 dose/day. The treatment course of both groups lasted for 4 weeks. Efforts were made to detect gastric emptying by B ultrasonic before and after treatment, record gastric half emptying time(T1/2 ), gastric emptying time(T), score the symptom severity index (GCSI) of the stomach and mild paralysis, and test the levels of plasma motilin and gastrin before and after treatment. Result:Total effective rate in observation group was 91.8%, which was superior to 74.4% in control group (P<0.05). After treatment, gastric half emptying time and gastric emptying time in observation group were shorter than these in control group (P<0.05). Postprandial abdominal distension/early full, nausea and vomiting, abdominal distension score and gastroparesis total score in observation group were all lower than these data in control group (P<0.01). Levels of motilin and gastrin in observation group were lower than the standards in control group (P<0.01). Conclusion:Oral administration of Jiawei Yigong powder can reduce clinical symptoms of DGP patients, regulate gastrointestinal hormone, promote the gastric emptying, improve the clinical curative effect, and so is worth further studies.关键词：diabetic gastroparesis;jiawei Yigong powder;gastric emptying;gastrin;motilin
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发布时间：2024-01-04 - 摘要：Objective:To discuss short-term effect of iontophoresis therapy of Juanbi decoction combined with mobilization techniques in treating knee osteoarthritis. Method:One hundred and twelve patients were randomly divided into control group (55 cases) and experimental group (57 cases) by random number table. Patients in control group were treated with middle and low frequency electric therapy apparatus and mobilization techniques. Besides the treatment of control group, patients in experimental group were additionally treated with iontophoresis treatment of Juanbi decoction. One course of treatment last for 2 weeks, both groups took four courses, with a break of 2-3 days between every course. Before and after treatment, the Western Ontario and McMaster Universities (WOMAC) osteoarthritis indexes, Visual Analogue Scale (VAS) pain scores and signs of knee joint and arthritis life quality measurement scale2-short form (AIMS2-SF) life quality scale were scored. Result:The total effective rate was 94.74% in experimental group, which was superior to 81.82% in control group (P<0.05). After treatment, the scores of WOMAC pain, stiffness and joint function and total score in experimental group were all lower than theses data in control group (P<0.01). Scores of VAS score and the knee joint symptoms in experimental group were inferior to these in control group (P<0.01). The five dimension scores as AIMS2-SF body scale, symptoms, influences, social and work, and total scores in experimental group were all higher than these in control group after treatment (P<0.01). Conclusion:Iontophoresis of Juanbi decoction combined loose joint technique to treat knee osteoarthritis can reduce pain, improve body signs, promote joint function recovery and enhance patients' quality of life. Its short-term curative effect is distinct.关键词：knee osteoarthritis;Juanbi decoction;joint mobilization;iontophoresis;life quality
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the efficacy and safety of compound Kushen injection for cancer-related pain, and provide information for clinical application and traditional Chinese medicine(TCM) injection research. Method:Computer retrieval of randomized controlled trials (RCTs) on compound Kushen injection for cancer-related pain was done. No language or blinding restriction was used. Quality of the literature and methodological quality of RCTs was assessed According to the methods recommended by Cochrane center. RevMan 5.3 was used for Meta-analysis on the efficacy and safety of compound Kushen injection. Result:The 21 randomized controlled trials were included in the study, involving 1903 patients, and 12 literature were used for Meta-analysis according to different treatment groups combined with analgesic. Compared with the western analgesic alone,compound Kushen injection combined with western analgesic had the following relative risk (RR) and 95% CI were[n=1115, RR=1.15,95%CI(1.09,1.21)], P<0.00001]. In respect of adverse effects, the combined effects showed that the RR and CI were as follows:constipation[n=481, RR=0.50, 95%CI(0.33, 0.78), P=0.002], nausea[n=421, RR=0.49, 95%CI(0.24, 0.99), P=0.05], vomit[n=208, RR=0.33, 95%CI(0.09,1.17), P=0.09], dysuria[n=268, RR=0.38, 95%CI(0.09, 1.60), P=0.19]. There were no serious adverse events. Conclusion:Compound Kushen injection combined with western analgesic had better efficacy than analgesic alone, and could assisted to reduce the adverse events of analgesic. Although in recent years, the quantity and quality of RCTs have a tendency to increase year by year, the research level remains to be enhanced through scientific design and normative report.关键词：compound Kushen injection;cancer-related pain;systematic review;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective:To study the safety and efficacy of traditional Chinese medicine(TCM) dialectical treatment combination with chemotherapy in advance non-small cell lung cancer(NSCLC). Method:We searched The Cochrane Library, Pubmed, Embase, VIP, Wangfang Data,CNKI,CBM, from database inception to July5, 2015.Randomized controlled trials (RCTs) of TCM dialectical treatment combination with chemotherapy in advance non-small cell lung cancer in any language were included, and recover into research references. Two researchers extracted data and assessed the literature quality separatedly, and made a Meta analysis RevMan 5.3 software. Result:The 12 RCTs involving 1341 patients entered the inclusion criteria. The Meta-analysis results showed that compared with the pure application of chemotherapy regimens, TCM dialectical treatment combination with chemotherapy can significantly improve the patients quality of life[OR=1.58,95%CI(1.03,2.42),P=0.03], curative effect in the near future[OR=4.38,95%CI(3.17,6.05),P<0.00001]. Adverse reactions, TCM dialectical treatment combination with chemotherapy can reduce bone marrow suppression caused by chemotherapy (white blood cells decline[OR=0.21,95%CI(0.10,0.44),P<0.0001], hemoglobin decline[OR=0.29,95%CI(0.13,0.68),P=0.004], platelet decline[OR=0.26,95%CI(0.10,0.69),P=0.007], digestive tract reactions[OR=0.23,95%CI(0.09,0.63),P=0.004],but in the liver function damage[OR=0.37,95%CI(0.08,1.66),P=0.19], renal impairment[OR=0.51,95%CI(0.23,1.14),P=0.10] were no significant differences. Conclusion:The current results indicates that TCM dialectical treatment combination with chemotherapy in advance non-small cell lung cancer can effectively improve the quality of survival, near future curative effect,reduce severe bone marrow suppression caused by chemotherapy, and gastrointestinal adverse reactions,but liver and kidney function damage and simple scheme there was no significant difference compared with chemotherapy. However, the efficacy and safety of TCM dialectical treatment combination with chemotherapy in advance non-small cell lung cancer still need more strict, large sample, randomized, double-blind trials to verify.关键词：traditional Chinese medicine dialectical treatment;chemotherapy;non-small cell lung cancer;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective:To analyze the composition and compatibility principles of the Chinese medicine that can prevent and treat obesity. Method:The 167 valid references were included after retrieving the Chinese biomedical literature database (CBM) according to the research purpose. We collected the information related to disease syndrome, prescription, and Chinese medicine from clinical treatment of traditional Chinese medicine (TCM) literature by artificial way. Then we analyzed the compatibility characteristics of Chinese medicine using frequency statistics, association rules and complex network analysis methods. Result:We screened out 209 prescriptions for obesity in clinical used, and identified the top ten high-frequency commonly used medicines. 588 Chinese medicinal combinations were obtained by association rules, and the core herbs and basic prescriptions for obesity were determined preliminarily. Conclusion:Poria, Alismatis Rhizoma, Atractylodis Macrocephalae Rhizoma and Crataegi Fructus were core herbs for obesity by analyzing. The mechanism was related to replenishing spleen and Qi, expelling dampness and phlegm, and supplemented with cathartic, Qi-regulating drugs, and blood activating herbs according to actual pathogenic condition to achieve the purpose of curing.关键词：obesity;data mining;composition and compatibility principles
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发布时间：2024-01-04 - 摘要：Objective:To summarize the prescription rules of traditional Chinese medicine(TCM) for treating chronic heart failure in CNKI based on TCM inheritance platform (V2.5) software. Method:We collected the literature about TCM prescriptions for treating chronic heart failure from CNKI, established a prescription database by Traditional Chinese Medicine Inheritance Platform System, and figured out the prescription rules. Result:Totally 101 prescriptions were collected in the treatment of chronic heart failure, and 131 herbs were used. Among them, the medicines for invigorating Qi and warming Yang, promoting urination, promoting blood circulation and removing blood stasis were frequently used. The most frequently used drug combination was Astragalus Mongholicus-Semen Lepidiies, which appeared for 40 times, evolving into 16 core medicine combinations and 8 new prescriptions. Conclusion:TCM prescriptions for chronic heart failure are mainly to warm Yang and invigorate Qi, induce diuresis and alleviate edema, promote blood circulation and remove blood stasis, which reveals the prescription rules, and provides the reference for the clinical treatment.关键词：chronic heart failure;traditional Chinese medicine inheritance platform system;prescription rules
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发布时间：2024-01-04 - 摘要：Objective:To analyze the prescription rules in external use for knee osteoarthritis by using Traditional Chinese Medicine(TCM)Inheritance Support System. Method:TCM external use prescriptions in the literature of last 15 years for treating knee osteoarthritis were collected. Association rules algorithm, complex system entropy clustering and other unsupervised data mining methods were used to analyze the frequency of various herbs and association rules between the herbs and dig for core combinations and new prescriptions. Result:The prescriptions were mainly of relaxing the muscles and stimulating the blood circulation or Haitongpi decoction;18 core combinations and 9 new prescriptions were dig out. Conclusion:Commonly used external use drugs for knee osteoarthritis are blood circulation drugs, rheumatism medicine, mainly to eliminate wind and dampness, promote blood circulation and remove blood stasis, warm channel and expel cold.关键词：knee osteoarthritis;Traditional Chinese Medicine Inheritance Auxiliary Systems;prescriptions;external medicine
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the effectiveness and safety of Yimucao injection combined with oxytocin in preventing postpartum hemorrhage during cesarean section. Method:Search was conducted in the following databases:PubMed, EMbase, Cochrane Library, CNKI, VIP and WanFang Data during the period from the established time to September 2014.Randomized controlled trial (RCT) of Yimucao injection combined with oxytocin in preventing postpartum hemorrhage during cesarean section were included, and its adverse drug reaction, postpartum bleeding and postpartum hemorrhage rate were observed. The quality of included studies was assessed according to Cochrane Methods 5.1 for systematic review, and Meta-analysis was conducted using RevMan 5.1 software. Result:A total of 25 studies involving 5709 patients were included. The Meta-analysis results indicated significant difference in intra-operative bleeding volume between Yimucao injection combined with oxytocin group and control group[MD=-44.96, 95%CI(-64.15,-25.76), P<0.00001];showed significant difference in 2 hours postoperative bleeding between the two groups[MD=-38.25, 95%CI(-51.59,-24.90), P<0.00001];showed significant difference in 24 hours postoperative bleeding between the two groups[MD=-49.26,95%CI(-65.66,-32.85), P<0.00001]. The Meta-analysis showed a significant reduction in the risk of postpartum hemorrhage rates between two groups[OR=0.33, 95%CI (0.25, 0.43), P<0.00001]. Side effect rates showed no significant difference between two groups[OR=0.78, 95%CI(0.41,1.47), P=0.44]. Conclusion:Compared with control group, Yimucao injection combined with oxytocin in preventing postpartum hemorrhage during cesarean section can significantly reduce postpartum bleeding and postpartum hemorrhage rate, without increasing the side effects for patients. We conclude that Yimucao injection combined with oxytocin is more appropriate for preventing postpartum hemorrhage during cesarean section. However, due to the limitation of both quantity and quality of included studies, this conclusion should be further confirmed with more high quality and large multicenter randomized controlled clinical trials.关键词：Yimucao injection;oxytocin;cesarean section;postpartum hemorrhage;systematic review;Meta-analysis
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发布时间：2024-01-04 - 摘要：To sort out and analyze the literature on the damage mechanism of angiotensin Ⅱ to glomerular endothelial cells. Angiotensin Ⅱ, glomerular endothelial cells, endothelial cell injury were used as the keywords to search for the relevant literature on internal and abroad studies of glomerular endothelial cell damage mechanism in recent 10 years, and then the analysis was conducted. The results showed that angiotensin Ⅱcould cause glomerular endothelial cell injury through activating inflammatory reaction and oxidative stress, inducing apoptosis, injurying structure of glomerular endothelial cells and endocrine functions, as well as changing the dynamics of the renal blood flow and other ways. This study has great significance to further clarify the injury mechanism of angiotensin Ⅱto glomerular endothelial cells and find effective prevention and treatment methods.关键词：angiotensin II;glomerular endothelial cells;mechanism
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发布时间：2024-01-04 - 摘要：Pomegranate is the homology of medicine and food medicine, has the anti-tumor, anti-inflammatory, anti-oxidation, fall blood sugar, blood pressure and prevent disease of heart head blood-vessel and so on the many kinds of pharmacological and physiological function. Therefore, in recent years, more and more research and exploration on pomegranate have drawn the attention of the related areas around the world. Pomegranate cultivation in our country has a long history and rich in resources, but at present domestic scholars of pomegranate research mostly focus on the extraction process of polyphenols and flavonoids reported separation, chemical composition and pharmacological action in vitro, etc., the pomegranate monomer compounds in anti-tumor research reported less.There are many effective active ingredient in pomegranate, including Ellagic acid,pomegranate glycosides,gallic acid, pomegranate acid, etc. In this paper, the research progress on the antitumor efficacy composition monomer compounds were reviewed, in order to provide reference for pomegranate antitumor effect of follow-up studies.关键词：pomegranate;anti-tumor;effect components
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发布时间：2024-01-04 - 摘要：Diffusing wave spectroscopy (DWS) is a kind of dynamic light scattering technology,it is a technology which lets multi-light beam into materials containing dispersed phase,and then photons through different distances are scattered and leads to interference,spectrum signal shows white light spot,transforming speed of spot reflects velocity of material particles.DWS technology applied in the high turbidity not more transparent medium particle size,structure and their interactions.In recent years,DWS technology is mainly used in chemical industry (emulsion film forming process analysis and mechanism research of latex particle film-forming process) and food industry (gel viscoelastic,stability determination of different milk in emulsion foods and rheological properties characterization of non-emulsion food),but application in medical literature reported less,it is mainly used for characterization of vascular blood flow and blood microcirculation,particle size analysis of suspension polymer in medicine.By investigating principle and application of DWS,this paper will prospect its development in medicine.关键词：diffusion wave spectroscopy;particle motion;dynamic light scattering technology;Chinese patent medicine
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发布时间：2024-01-04 - 摘要：According to review of literature and marketing research,this paper introduced application history and trait,national application status and common quality control methods of Chinese herb powder,proposed solutions and thinking for these common problems.It was pointed in the paper that not all herbs were suitable to make into powder.Chemical components,molding process,pharmacology,toxicology,clinical medication feature and so on should be considered to study scientific nature.For problem that some fake materials may be put in the powder that cause difficulty of quality control,it was proposed that computer software and infrared spectroscopy technology should be adopted in quality evaluation system of Chinese herbal medicine powder,which can provide references for quality control of Chinese herbal medicine powder.关键词：Chinese herbal medicine powder;infrared spectroscopy technology;quality evaluation system;common problems;bulk density;ultrafine powder
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发布时间：2024-01-04 - 摘要：Malus hupehensis is a kind of traditional medicinal and edible plant,it is widely distributed in China and has a long history of application.Previous research found that M. hupehensis is rich in protein,polyphenols,flavonoids and trace elements,it has kinds of pharmacological activities,such as antioxidant,hypoglycemic,anti-fatigue and liver protection.What's more,M. hupehensis can be used as a raw material extracted phloridzin,and be developed as potential plant resources of diabetes drugs.As a result,M. hupehensis has a broad prospect in the development and utilization of medicines,beverages and health care products.In order to use M. hupehensis better,this article summaries research progress of its chemical constituents and pharmacological activities.关键词：Malus hupehensis;chemical constituents;pharmacological activities;phloridzin;total flavonoids
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发布时间：2024-01-04 - 摘要：It is can' t guarantee the quality of Chinese traditional drugs completely, only by establishing the quality standard ofmedicinal material and decoction pieces. Now, we put forward a process control system (PCS) including every process and link in the production of Chinese traditional drugs by means of finding some problems and forming correlative measures. Based on 30 years for the systematic researches continuously on Trichosanthis Fructus, a commonly used Chinese traditional drug, we put forward a new concept of CPS on the quality of TrichosanthisFructus. This concept of CPS involves 10 process and links, such as plant variety, cultivation and propagation, pest control, fruit-bearing and fruit' s maturity, storage, pharmacist, chemical constitute, quality standard, limiting index, and circulation, by means of finding a series of problems and forming correlative measures in these process and links of Trichosanthis Fructus. This CPS can ensure the quality of medicinal material and decoction pieces of Trichosanthis Fructus systematically and comprehensively, improve the clinical curative effect of traditional Chinese medicine, and provide a model for the establishment of CPS for other traditional Chinese drugs.关键词：Trichosanthis Fructus;process control system;quality standard;storage;circulation
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