最新刊期
卷 22 ， 期 7 ， 2016
- 摘要：Objective: To prepare nasal dry powder inhaler, preparation of microspheres which assembled by baicalin nanoparticles with chitosan as carrier, in vitro release properties and nasal mucous membrane permeability were invesgated. Method: Primary nanoparticles were prepared by ionic cross-linking, nanoparticles were assembled to microspheres by spray drying method.Particles were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and so on, its in vitro release in artificial nose liquid electrolyte and permeability under simulated human nasal mucosa were investigated. Result: Average particle size of primary particles was (170.5±2.3) nm, while the second particles was (6.8±0.4) μm with drug dispersed as amorphous form in them.Specific energy of microspheres was (5.46±0.37) mJ · g-1, pressure drop was 0.323 kPa when air kept speed of 2 mm · s-1 through powder bed with force was 15 kPa.And the second particles could decomposed into primary nanoparticles in simulated nasal electrolyte solution.Cumulative release rate of baicalin was (78.85±2.71)% in 33 hours and release curve accorded with Riger-Peppas model.Value of cumulative release rate per unit area of microsphere was 1.77 times that of the pure baicalin sample. Conclusion: These prepared microspheres can release primary particles with good fluidity, permeability and a certain sustained-release effect, it can improve nasal mucous membrane permeability of baicalin.关键词：baicalin;ionic crosslinking;spray drying;microspheres;intranasal administraion
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发布时间：2024-01-04 -
Effect of Particle Size and Water Amount on Decoction of Magnoliae Officinalis Cortex Boiling Powder
摘要：Objective: To optimize partical size of Magnoliae Officinalis Cortex boiling powder, and investigate effect of the amount of water on decoction of this boiling powder. Method: Five kinds of particle size of Magnoliae Officinalis Cortex boiling powder were prepared.Contents of magnolol and honokiol in decoction were determined by HPLC, mobile phase consisted of methonl-water (30 : 70) with a flow rate of 1.0 mL · min-1 and detection wavelength was at 294 nm.With transfer rates of magnolol and honokiol, dry extract rate as indexes, effects of particle size and the amount of water on decoction of Magnoliae Officinalis Cortex boiling powder were investigated by single factor tests, and compared with traditional decoction of herbal pieces. Result: Particle size and water amount both had significant effect on compound content and dry extract rate in decoction of this boiling powder.Optimum preparation process was that particles must be all through 10 mesh sieve, and fine powder of 80 mesh sieve shall not exceed 20%.When boiling powder was decocted once with decoction time of 10 min, transfer rate of compound and dry extract rate of boiling powder (particles must be all through 24 mesh sieve, added 20 times the amount of water) were successively 5.1 and 2.5 times those in traditional decoction of herbal pieces (added 10 times the amount of water, decoction time of 50 min), those of boiling powder (particles must be all through 10 mesh sieve, 10 times the amount of water) were all 2 times those in traditional decoction of herbal pieces. Conclusion: Boiling powder of Magnoliae Officinalis Cortex boiling powder can save herbs and decoction time by comparing with traditional herbal pieces.关键词：Magnoliae Officinalis Cortex;boiling powder;traditional herbal pieces;particle size;water amount;magnolol;honokiol- 2
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发布时间：2024-01-04 - 摘要：Objective: To explore the best ratio of Danggui Buxue Tang for whitening efficacy by investigating whitening efficacy caused by different proportion of Astragali Radix and Angelicae Sinensis Radix in this compound. Method: Biochemical-enzymic method was adopted to measure tyrosinase inhibition rate of Danggui Buxue Tang and 1, 1-diphenyl-2-picrylhydrazyl(DPPH) method was adopted to measure its antioxidant activity.Franz diffusion cell test was used to evaluate skin permeation of Danggui Buxue Tang. Result: Optimum proportion of Astragali Radix and Angelicae Sinensis Radix in this compound was 1 : 7.Under this proportion, its inhibition rate of tyrosinase achieved 89.65% and scavenging ratio of DPPH free radicals achieved 90.96%.Accumulative permeation amounts in 24 h of ferulic acid and calycosin-7-O-β-D-glucoside were 0.030 4, 0.001 2 mg · cm-2, permeabilities were 59.27% and 57.79%, respectively. Conclusion: Change of compatibility ratio can affect efficacy of Danggui Buxue Tang, or even change status of compatibility between monarch and ministerial drug, then it may change syndrome and function of this prescription.关键词：uniform design;Danggui Buxue Tang;whitening efficacy;compatibility;tyrosinase
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different permeation enhancer on in vitro percutaneous absorption of naringin in Sanwei Dieda rheumatism gel paste. Method: Taking Franz diffusion cell to perform test, HPLC was adopted to determine the content of naringin in receiving liquid, effects of different permeation enhancer on in vitro percutaneous absorption of naringin in Sanwei Dieda rheumatism gel paste were investigated. Result: In vitro percutaneous absorption of naringin in this gel paste accorded with zero order release model, cumulative permeation equation was Q=1.126t+2.795 with transdermal rate of 1.126 μg · cm-2 · h-1.Either propylene glycol, azone or menthol was added, transdermal rates of naringin were improved, and effect was in order of menthol > propylene glycol > azone. Conclusion: Naringin in gel preparation can be percutaneous absorded.Propylene glycol, azone and menthol all can obviously promote transdermal absorption of naringin, especially for menthol, which plays the highest effect.关键词：naringin;percutaneous absorption;propylene glycol;Azone;Menthol
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发布时间：2024-01-04 - 摘要：Traditional Chinese medicine (TCM) preparations in medical institution are important part of TCM theory and practice.These preparations are able to provide different services for clinical and put in motion of characteristic therapy.Along with increasing of support from government, there is a good development opportunity accompanied some new challenges.Nowadays bottleneck problems are slow speed, high cost and inexistence of variety renewal.In order to break deadlock in research and development and explore a new mode which is in line with characteristics of TCM, we firstly attempt to research and develop a series of dozens of preparations, we set up an efficient research and development system, and then make research, development and clinical application of TCM preparation to be a dynamic process.Through analysis of a series of development practice in our hospital in recent years, we put forward viewpoint that research and development of TCM in medical institutions should be organized and planned transversely and longitudinally according to clinical requirements.Technological level should be enhanced at the same time.These works could promote improvement of medical technology of TCM.关键词：medical institution;traditional Chinese medicine preparations;series of research and development;planning
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发布时间：2024-01-04 - 摘要：Objective: To optimize water extraction process of Shenqi compound granules and investigate effects of different factor on the content of saponins in water extract and concentrated solution. Method: HPLC-ELSD was employed to determine contents of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and astragaloside Ⅳ at the same time.Based on single factor tests, taking solid-liquid ratio, extraction time and times as factors, orthogonal test was adopted to optimized extraction process with composite score of the content of saponins and yield of dry extract as index.Stability of Shenqi compound granules was investigated by determining the content of saponins in water extract and concentrated solution under different factor. Result: Optimum extraction process was as follows:extracted thrice with 10 times the amount of water, 40 min for each time.Extracting amounts of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and astragaloside Ⅳ were 1.556, 2.155, 2.168, 0.718 mg · g-1.Heating temperature and time was main factors affecting content change of saponins in Shenqi compound granules. Conclusion: High temperature and long time heating should avoid in preparation process of Shenqi compound granules, vacuum concentration method and vacuum drying method should be adopted to reduce degradation rate of ginsenosides.This optimized extraction process is feasible and suitable for industrial production of Shenqi compound granules.关键词：Shenqi compound granules;thermal stability;Ginsenoside Rg1;Ginsenoside Re;ginsenoside Rb1;astragaloside Ⅳ;yield of dry extract
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Shuanghuang Erxian granules. Method: HPLC was employed to determine contents of astragaloside Ⅳ and complanatuside in Shuanghuang Erxian granules with mobile phases of acetonitrile-water (35 : 65) and acetonitrile-0.2% phosphoric acid solution (21 : 79), respectively.Taking contents of astragaloside Ⅳ and complanatuside, yield of dry extract as comprehensive evaluation index, orthogonal test was used to investigate effects of water amount, extracting time and times on extraction process of Shuanghuang Erxian granules. Result: Optimum extraction condition was as follows:extracted twice with 8 times the amount of water, 1 hour for per time.Extracting amounts of astragaloside Ⅳ and complanatuside were 1.084, 1.343 mg · g-1, yield of dry extract was 28.48%, extraction time had a significant effect on extraction technology of Shuanghuang Erxian granules. Conclusion: This optimized extraction condition is stable and feasible, it can provide guidance for industrial production of Shuanghuang Erxian granules.关键词：astragaloside Ⅳ;complanatuside;yield of dry extract;Shuanghuang Erxian granules;composite score
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Jinshui Ditan oral liquid. Method: Single factor tests were adopted to optimize extraction technology of aromatic water with extraction rate of volatile oil as index.With composite score of extracting amounts of hesperidin and icariin, yield of dry extract as index, orthogonal test was adopted to investigate effects of the amount of water, decocting time and times on extraction process.HPLC was employed to determine contents of hesperidin and icariin. Result: Aromatic water extraction process was distilled 4 hours with 10-fold water at a speed of 2 seconds per drop, then distilled 2 hours from aromatic water at the same speed, the final volume of distilled fragrance water was about 1.1 times weight of medicine.Water extraction technology was as follows:decocted thrice with 8-fold water, each time for 1.5 hour;extracting amounts of hesperidin and icariin were 27.77, 15.30 mg · g-1, yield of dry extract was 40.13%. Conclusion: These optimized processes are stable and feasible, they can provide a reference for industrial production of Jinshui Ditan oral liquid.关键词：aromatic water;icariin;hesperidin;comprehensive evaluation method;dry extract yield
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发布时间：2024-01-04 - 摘要：Objective: To obtain the information about the usage of medicinal herbs in Miyi county of Sichuan province through the ethnobotanical investigation on medicinal market during dragon-boat festival, and provide basis for further scientific research, development and utilization. Method: Miyi county annals and relevant literature were read to make investigation table;herbal samples were collected and photos were taken in the medicinal market during dragon-boat festival for 2 continuous years;relevant details about these herbs' popular names, officinal organs, the therapeutic effects and their usage and dosage were recorded;variety identification and processing analysis were done with combination of the investigation data. Result: The 93 herbal medicines were traded in the medicinal market during dragon-boat festival, and 90 kinds were identified among them, involving 109 plant species, accounting for about 21% of the medicinal plants of the total county. These herbs were consisting of 45 families, 82 genera, including 1 kind of Fungi, 3 kinds of Ferns, 26 kinds of Monocotyledons, accounting for 24% of the total traded plants;80 kinds of Dicotyledons, accounting for 73% of the total. Conclusion: There are numerous herb species in Miyi county's medicinal market, and some of the local conventional species have significant effect, worthy of further development.关键词：Miyi county;medicinal market in dragon-boat festival;medicinal plants
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发布时间：2024-01-04 - 摘要：Objective: Change of basic physicochemical properties in rhizosphere soil was studied while fibrous roots of Salviae Miltiorrhizae Radix et Rhizoma were decayed naturally. Method: Contents of nutrient elements were measured by ICP-AES and contents of active ingredients in Salviae Miltiorrhizae Radix et Rhizoma were measured by HPLC. Result: Compared with the control group (CK), contents of Ca, Fe, K, Zn and N in the treatment 1 group (added fibrous roots of 20 g) decreased by 1.33%, 1.95%, 0.15%, 5.81% and 15.38%;they decreased by 4.10%, 3.62%, 2.47%, 5.81% and 53.85% in the treatment 2 group (added fibrous roots of 40 g), respectively.Contents of Cu, Mn and P in soil were positively related to the content of tanshinoneⅠ significantly;the content of K was positively related to contents of tanshinoneⅠ and tanshinon ⅡA significantly in the same time;the content of Ca was positively related to dry weight on the ground of Salviae Miltiorrhizae Radix et Rhizoma significantly;the content of Zn was positively related to root fresh weight significantly;the content of Fe was negatively related to the content of rosemary acid significantly. Conclusion: Contents of mineral elements in rhizosphere soil have a certain relation to yield or quality of Salviae Miltiorrhizae Radix et Rhizoma. Fibrous roots decomposition can affect contents of mineral elements in rhizosphere soil of this herb, which is the reason that its yield and quality changed under different treatment.关键词：Salviae Miltiorrhizae Radix et Rhizoma;fibrous roots decomposition;active ingredients;yield;inorganic elements
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical components of Gardeniae Fructus. Method: Various column chromatographies were used in the isolation and purification, physiochemical constant determination and spectral analysis were adopted to determine the chemical structures. Result: Ten compounds were isolated and identified as shanzhiside (1), deacetylasperulosidic acid (2), 8-hydroxy-pentadecanoic diacid (3), myo-inositol (4), densispinin D (5), 3, 4-dihydroxypipecolic acid (6), 3-hydroxypipecolic acid (7), α-D-glucopyranosyl-(1→1')-3'-amino-3'-deoxy-β-D-glucopyranoside (8), β-D-xylopyranosyl-(1→6')-O-β-D-glucopyranoside (9), β-D-galacopy-ranosyl-(1→6')-O-β-D-glucopyranoside (10). Conclusion: Compounds 2-10 were isolated from this plant for the first time.关键词：Gardeniae Fructus;chemical constituents;deacetylasperulosidic acid
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发布时间：2024-01-04 - 摘要：Objective: To study the effective substance basis of leaves of Murraya exotica. Method: Preparative TLC, repeated silica gel chromatograph, half-making HPLC were used to separate and purify the isolated compounds, which were structurally identified by the physical and chemical characters and the spectrum data. Result: Eleven compounds were obtained. They were 5, 7, 3', 4'-tetramethoxyflavone (1), 5, 7-dihydroxy-3', 4', 5'-trimethoxyflavone (2), 5-hydroxy-3, 7, 3', 4'-tetramethoxyflavone (3), 3-hydroxy-5, 6, 7, 4'-tetramethoxyflavone (4), nobiletin (5), vanillic acid (6), ethyl caffeate (7), 5-hydroxy-7, 3', 4'-trimethoxyflavone (8), 3, 5-dihydroxy-6, 7, 3', 4'-tetramethoxyflavone (9), 5, 6, 4-trihydroxy-3, 7-dimethoxyflavone (10), and kaemperol (11). Conclusion: The compounds 3, 4, 6, 9 and 10 were isolated from M.exotica for the first time.关键词：Murraya exotica;chemical constituents;structural identification;5-hydroxy-3,7,3',4'-tetramethoxyflavone
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发布时间：2024-01-04 - 摘要：Objective: To study on the chemical constituents of Diaphragma Juglandis Fructus and investigate the antioxidant activity of their monomeric compounds. Method: Various column chromatography methods were used to isolate and purify the compounds and their structures were identified on the basis of spectral data. In addition, the antioxidant activities of compounds were evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. Result: Seven monomeric compounds were isolated from ethanol extracts of Diaphragma Juglandis Fructus and their structures were identified as dihydrovomifoliol-9-O-β-D-glucopyranoside (1), taxifolin-3-O-α-L-arabinofuranoside (2), quercetin-3-O-(4"-O-acetyl)-α-L-rhamnopyranoside (3), kaempferol-3-O-α-L-rhamnopyranoside (4), kaempferol (5), naringenin (6) and vanillin (7). Compounds 1-5 and 7 were isolated from Diaphragma Juglandis Fructus for the first time. As the pathogenesis of kidney disease is closely related to the body's disorder of antioxidant balance, in order to illustrate the material basis for kidney-reinforcing efficacy of Diaphragma Juglandis Fructus, three phenolic acid compounds and five flavonoids compounds were investigated for their antioxidant activity using DPPH radical scavenging assay combined with the previous experimental results. All the compounds indicated radical scavenging capacity to different extents. Conclusion: The chemical constituents of Diaphragma Juglandis Fructus have certain antioxidant activities, providing theoretical basis for the elaboration of its traditional efficacy.
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Adenophorae Radix. Method: The compounds were isolated and purified by silica gel column, sephadex LH-20 column chromatography. Their structures were identified by physicochemical properties and spectral data. Result: Thirteen compounds have been isolated from the petroleum ether, ethyl acetate and n-butanol fractions. They were respectively identified as α-amyrin acetate (1), taraxerone (2), lupenone (3), β-sitosterol (4), lupeol acetate (5), sessilifolic acid 3-O-isovalerate (6), stigmastane (7), n-pentadecanoic acid (8), bis(2-ethylhexyl) phthalatel (9), wogonoside (10), guaiacol β-D-glucopyranoside (11), 5-hydroxymethyl furfural (12), baicalin (13). Conclusion: Compound 1, 8, 10, 11, 13 were obtained from the Campanulaceae for the first time, compound 9, 12 were obtained from the Adenophora for the first time, and compound 6 was obtained from this plant for the first time.关键词：Adenophorae Radix;chemical constituents;structure identification;sessilifolic acid 3-O-isovalerate
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Notoginseng Radixet Rhizoma. Method: The compounds were isolated and purified by silica gel and Sephadex LH-20 chromatography. Their structures were identified by physicochemical properties and spectral analysis. Result: Ten compounds were obtained and identified as slauric acid (1), β-sitosterol (2), (3R, 9R, 10R)-panaxytriol (3), monolaurin (4), β-daucosterol(5), (8R, 9R, 10S, 6Z)-trihydroxyoctadec-6-enoic acid (6), 20(S)-ginsenoside Rh1 (7), 20(R)-ginsenoside Rh1 (8), 20(S)-ginsenoside Rg1 (9), notoginsenoside R1 (10). Conclusion: Compounds 1, 4, 6 are isolated from this plant for the first time.关键词：Notoginseng Radixet Rhizoma;slauric acid;monolaurin;(8R, 9R, 10S, 6Z)-trihydroxyoctadec-6-enoic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprints of Liparis nervosa and provide the basis for scientific evaluation and quality control of L. nervosa. Method: HPLC method was used on Welchrom-C18(4.6 mm×250 mm, 5 μm) chromatographic column, with Acetonitrile-0.2% triethylamine solution (10% phosphoric acid solution, pH adjusted to 2.5) as gradient elution. The wavelength was detected as 254 nm;the flow rate was 1.0 mL · min-1, and the column temperature was 30 ℃. The HPLC fingerprints were established for the L. nervosa from 10 different origins. At the same time, the similarity evaluation system for chromatographic fingerprint of TCM (2004A version) was applied for similarity evaluation. Besides, cluster analysis was done by using SPSS 19.0 software. Result: Twenty-eight common peaks were calibrated from the HPLC fingerprints of L.nervosa, six of which were identified by reference substances (3 alkaloids and 3 flavonoids). In the similarity evaluation, L. nervosa from 8 of 10 origins had a similarity ratio of more than 0.9 when compared with the mutual mode HPLC fingerprints. The overall similarity was good, and the cluster analysis result was basically the same as the similarity evaluation result. However, the similarity ratio of the materials from Mei county in Guangdong was only 0.173 and that of Ya'an in Sichuan was 0.320.These differences may be caused due to weather conditions, regional differences and different harvest seasons. Conclusion: This method is highly sensitive, reproducible, simple and reliable, and can be used for quality control of L. nervosa.关键词：Liparis nervosa;HPLC;fingerprint;similarity evaluation;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of ethanol extract of Astragalus membranaceus var. mongholicus. Method: The chemical constituents were isolated and purified by various chromatographic techniques and the structures were determined by physico-chemical properties and modern spectroscopic techniques. Result: Twelve compounds were isolated from the ethanol extract of A. membranaceus var. mongholicus, including β-sitosterol (1), daucosterol (2), formononetin (3), oroxylin-A (4), wogonin (5), calycosin (6), adenosine (7), calycosin-7-O-β-D-glycoside (8), (6αR, 11αR)-9, 10-dimethoxy-pterocarpan-3-O-β-D-glycoside (9), astragalosideⅡ (10), isoastragalosideⅡ (11), D-3-methoxy-chiro-inositol (12). Conclusion: Compounds 3 and 4 were isolated from Astragalus membranaceus var. mongholicus for the first time.关键词：Astragalus membranaceus var. mongholicus;chemical constituents;oroxylin-A;wogonin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical components of Thesium chinense. Method: Various column chromatography methods were used to isolate and purify the compounds from 95% ethanol extracts of T. chinense, and their structures were identified by physico-chemical and spectral analysis. Result: Ten compounds were isolated from T. chinense and identified as luteolin-7-O-glucoside (1), apigenin-7-O-glucoside (2), rutin (3), apigenin-8-C-α-L-arabinopyranoside (4), homoplantaginin (5), pectolinarin (6), linarin (7), apigenin-7-O-β-D-glucopyranuronide (8), chrysoeriol 7-O-glucuronide (9) and chrysoeriol (10). Conclusion: Compound 10 was isolated from the genus Thesium for the first time and compounds 4-9 were isolated from the family Santalaceae for the first time.关键词：Thesium chinense;Santalaceae;homoplantaginin;pectolinarin;linarin;chrysoeriol
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发布时间：2024-01-04 - 摘要：Objective: To explore the chemical constituents of the Cassia mimosoides. Method: Silica gel chromatography, gel chromatography, and reverse phase column chromatography were used for separation and purification. The structures were identified on the basis of spectral data and physicochemical examination. Result: Twelve compounds were isolated from C. mimosoides and their structures were identified as vanillic acid(1), luteolin-7-O-β-D-glucoside(2), luteolin(3), chrysophanol-gentiobioside(4), 5, 7, 4'-trihydroxy-3'-methoxyflavone(5), quercetin(6), kaempferol(7), oleanolic acid(8), emodin(9), physcion(10), daucosterol(11), β-sitosterol(12). Conclusion: Compounds 5 was isolated from in Cassia for the first time and compounds 1, 4, 6, 7 were isolated from C. mimosoies for the first time.关键词：Cassia mimosoides;separation and identification;5,7,4'-trihydroxy-3'-methoxyflavone
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from Salvia chinensis. Method: The compounds were isolated and purified by using various chromatographic techniques, including c silica gel olumn chromatography, Sephadex LH-20, ODS and preparative HPLC. Their structures were identified by spectroscopic techniques, including NMR and MS. Result: Eight compounds were isolated from ethanol extract of S. chinensis, and their structures were identified as emodin (1), chrysophanol (2), deoxyschizandrin (3), gomisin N (4), oleanolic acid (5), ursolic acid (6), β-sitosterol (7) and daucosterol (8). Conclusion: Compounds 1, 3 and 4 were isolated from Lamiaceae for the first time, and compound 2 was isolated from Salvia for the first time.关键词：Salvia;S. chinensis;chemical constituents;chrysophanol
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发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprints of flavonoids in Ophiopogonis Radix of Zhejiang and simultaneously determine the contents of methylophiopogonanone A and methylophiopogonanone B. Method: The chromatographic method was carried out on Kromasil 100-5 C18 column (4.6 mm×250 mm, 5 μm) using acetonitrile-0.1%phosphoric acid (58 : 42) as the mobile phase. The flow rate was 1.0 mL · min-1;and the column temperature was 30 ℃. Result: The HPLC fingerprints of Ophiopogonis Radix of Zhejiang were established and 7 common peaks were defined in the HPLC fingerprints. The similarity among different batches was above 0.9.By HPLC-MS analysis and comparison with standard reference substance, No. 1 peak was determined as methylophiopogonanone A and No. 2 peak was determined as methylophiopogonanone B. In the quantitative analysis of these two compounds, we found that the linear range was 31.47-786.8 ng for methylophiopogonanone A (R2=1.000 0) and 53.05-1 326.25 ng for methylophiopogonanone B (R2=1.000 0), and the average recovery rate (n=6) was 101.5% for methylophiopogonanone A and 103.0% for methylophiopogonanone B. Conclusion: The HPLC fingerprint method and content determination method are simple, accurate with good reproducibility, and can be applied in the quality control of Ophiopogonis Radix of Zhejiang.关键词：Ophiopogonis Radix of Zhejiang;methylophiopogonanone A;methylophiopogonanone B;HPLC fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of n-butanol fraction of notoginseng medicinal fungal substance. Method: The compounds were separated and purified through the system solvent extraction, D101 macroporous adsorbent resin, silica gel column chromatography, open ODS column chromatography, and semi-preparative liquid chromatography. The structures of the compound were identified by chemical properties and spectroscopic data. Result: Six compounds were separated from n-butanol fraction of notoginseng medicinal fungal substance and identified as ginsenoside Rk3(1), pseudoginsenoside RT2(2), ginsenoside Rg3(3), 24(R)-pseudoginsenoside GQ (4), ginsenoside Rd (5), and gypenoside LXIX (6). Conclusion: Compound 2 and compound 4 were isolated from notoginseng for the first time, and all compounds were isolated from notoginseng medicinal fungal substance for the first time.关键词：notoginseng medicinal fungal substance;n-butanol fraction;pseudoginsenoside RT2;24(R)-pseudoginsenoside GQ
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of n-butanol fraction of notoginseng medicinal fungal substance which was obtained from notoginseng by biotransformation technology. Method: The compounds were isolated and purified through system solvent extraction, D101 macroporous adsorptive resin, silica gel column chromatography, open ODS column chromatography, and semi-preparative liquid chromatography. The structures of these isolated compounds were identified by means of chemical properties, spectroscopic analysis. Result: Six compounds were separated from n-butanol fraction of notoginseng medicinal fungal substance and identified as 24(R)-pseudoginsenoside RT5(1), ginsenoside SG1(2), ginsenoside Rh12(3), ginsenoside Rb1(4), vina-ginsenoside R8(5), and vina-ginsenoside R9(6). Conclusion: Compounds 1, 2, 5 and 6 were isolated from this plant for the first time, and all compounds were isolated from notoginseng medicinal fungal substance for the first time.关键词：notoginseng medicinal fungal substance;n-butanol fraction;24(R)-pseudoginsenoside RT5;ginsenoside SG1;vina-ginsenoside R8;vina-ginsenoside R9
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for simultaneous determination of ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rh1, ginsenoside Rb1, ginsenoside Ro, ginsenoside Rb2, and ginsenoside Rd in ginseng medicinal fungal substance. Method: High performance liquid chromatography was carried out on Inertsil ODS-SP chromatographic column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.4% phosphate as the mobile phase for gradient elution. The flow rate was 1 mL · min-1, and detection wavelength was 203 nm. The column temperature was at 30 ℃. Result: Eight kinds of monomer saponins could be separated well in 100 min, with good linear relationship (R2>0.999 7).The average recovery rate was 100.15% (RSD 1.6%), 101.54% (RSD 2.3%), 101.87% (RSD 1.9%), 100.88% (RSD 2.1%), 102.10% (RSD 1.5%), 102.84% (RSD 0.8%), 101.38% (RSD 2.7%), and 101.23% (RSD 1.6%). Conclusion: The HPLC method is accurate, reliable and reproducible to analyze 8 saponins in ginseng medicinal fungal substance, which can provide the scientific basis for formulating the quality standard of the research on composition transformation of the ginseng medicinal fungal substance.关键词：ginseng medicinal fungal substance;Ginsenoside Rg1;Ginsenoside Re;ginsenoside Rf;ginsenoside Rh1
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发布时间：2024-01-04 - 摘要：Objective: To research change of the content of alkaloids of Ephedrae Herba in plasma after oral administration of different compatibility proportion of drug pair of Ephedrae Herba-Glycyrrhizae Radix et Rhizoma aqueous extracts in rats. Method: Rats were randomly divide into Mahuang (MH) group, MH-GC (12 : 3) group, MH-GC (12 : 6) group and MH-GC (12 : 12) group by weight, plasma samples were collected at different time after drug administration.Plasma samples were treated with liquid-liquid extraction, with amantadine as internal standard, concentrations of norephedrine hydrochloride (NME), norpseudoephedrine hydrochloride (NMP), ephedrine hydrochloride (E), pseudoephedrine hydrochloride (PE), methylephedrine hydrochloride (ME) in plasma samples at different time were determined by UPLC-MS/MS, DAS 3.0 software was used to calculate pharmacokinetic parameters. Result: Compared with MH group, MRT0-t of NME and NMP were significantly reduced in MH-GC (12 : 3) group, t1/2z and Vz/F of PE had the same trend;AUC0-t of NME, E and PE were significantly increased, CLz/F and Vz/F of PE were significantly reduced in MH-GC (12 : 6) group;MRT0-t of E and PE were significantly extended, CLz/F of ME were significantly increased in MH-GC (12 : 12) group. Conclusion: After compatibility with Glycyrrhizae Radix et Rhizoma, mian pharmacokinetic parameters of five alkaloids in Ephedrae Herba have significant differences.And follow the amount of Glycyrrhizae Radix et Rhizoma, focus of attenuated or efficiency is different.This study demonstrates rationality of ancient prescriptions from pharmacokinetics.关键词：drug pair of Ephedrae Herba-Glycyrrhizae Radix et Rhizoma;compatibility;alkaloids;pharmacokinetics;ephedrine;pseudoephedrine
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Mongolia medicine Zhonglun'e Tang on the expressions of matrix metalloproteinase-3 (MMP-3) and MMP-9 in rats with collagen induced arthritis (CIA) and to study its treatment mechanism for rheumatoid arthritis (RA). Method: arthritis rat models were established by using type Ⅱ collagen. The rats were divided into normal group, model group, tripterygium wilfordii polycoride tablets group (9.45 mg · kg-1), Mongolian medicine Zhonglun'e Tang high dose (5.4 g · kg-1), medium dose (2.7 g · kg-1) and low dose (1.35 g · kg-1) groups. The drugs were given for 4 weeks, and then HE staining was used to observe the histopathologic morphology of synovial tissues. MMP-3 and MMP-9 levels in the serum of rats were measured by enzyme linked immunosorbent assay (ELISA). The expressions of MMP-3 and MMP-9 in the synovial tissues were detected by using immunohistochemistry and Western blot. Result: The levels of MMP-3 and MMP-9 in serum of model group were significantly higher than those in normal group (P<0.01);positive expressions of each drug group were lower than those in model group to varying degrees (P<0.01), but there was no significant difference between Zhonglun'e Tang high dose group and tripterygium wilfordii polycoride tablets group. The results of immunohistochemistry method showed that, the expressions of MMP-3 and MMP-9 in mode group were significantly higher than those in normal group (P<0.01), and there was statistically significant difference between Zhonglun'e Tang high dose group, tripterygium wilfordii polycoride tablets group were lower in protein expression of MMP-3 and MMP-9 than those in model group(P<0.05). The results of Western blot showed that the protein expressions of MMP-3 and MMP-9 in the treatment groups group were significantly lower than those in model group, with statistically significant difference (P<0.05). Conclusion: Zhonglun'e Tang could significantly decrease the MMP-3 and MMP-9 levels in serum of CIA rats, and reduce the positive expressions of synovial tissues. This may be the mechanism for treating RA.关键词：Zhonglun'e Tang;type Ⅱ collagen induced arthritis;rheumatoid arthritis;metalloproteinase-3;metalloproteinase-9
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of compatibility of aconitine and ginsenosides Rb1 on ATP enzyme and relatedion of myocardial cells. Method: Tryptic digestion and differential adherence method were used to culture myocardial cells in new born rats, and then these rats were divided into normal group, aconitine group, ginsenosides Rb1group, and aconitine and ginsenosides Rb1 compatibility (1 : 1, 1 : 2, 2 : 1) groups. After treatment for one hour, the activity of myocardial cells, ion concentration and ATP enzyme activity in myocardial cells were detected in various groups. Result: The 0.2% aconitine could reduce myocardial cells activity, reduce myocardial intracellular Mg2+ and K+ concentration, increase concentration of Ca2+ and Na+, and reduce Ca2+-ATPase, Na+-K+ ATPase and Ca2+-Mg2+-ATPase activities;compatibility of aconitine and ginsenosides Rb1 could increase myocardial intracellular Mg2+ and K+ concentration, reduce myocardial intracellular Ca2+ and Na+ concentration, and improve Ca2+-ATPase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities. Conclusion: Ginsenosides Rb1 can reduce the aconitine toxicity on cardiomyocytes, and mechanism may be associated with regulating ATPase activity of myocardial cell membrane and related ion concentration in cells.关键词：ginsenosides Rb1;aconitine;Myocardial cells;ATPase
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of different concentrations of Shuanghuanglian injection (SHLI) and its intermediates on proliferation of RBL-2H3 cells, and provide evidence for establishing anaphylactoid cell models and monitoring drug quality. Method: RBL-2H3 cells were treated with different concentrations of SHLI (2 times gradient dilution from volume ratio 1: 16 to 1: 4 096) and its intermediates (2 times gradient dilution from volume ratio 1: 8 to 1: 2 048). The cellular morphology was observed under inverted microscope. MTT colorimetric assay was used to determine the growth of RBL-2H3 cells in different concentration of SHLI and its intermediates, and the half inhibitory concentration (IC50) was calculated. Dependent on the value of IC50, RBL-2H3 cells were then treated with different concentrations of SHLI to observe the effect of SHLI on cells apoptosis. Result: As the concentration increases of SHLI and its intermediates, their inhibitory effect on proliferation of RBL-2H3 cells was gradually increased; the cells survival rate was decreased significantly; cells injury was gradually aggravated; and the cellular morphology was also changed. High dose of SHLI could induce apoptosis of RBL-2H3 cells. Conclusion: SHLI and its intermediates could reduce RBL-2H3 cells proliferation, we can provide certain experiment basis for establishing anaphylactoid cell models and monitoring drug quality and safety.关键词：Shuanghuanglian Injection;intermediate products;RBL-2H3;cell proliferation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of different components of Acori Tatarinowii Rhizoma-Polygalae Radix (volatile oil, aqueous extract) on learning and memory capacity as well as hippocampal synaptic proteins expression in Alzheimer's disease (AD) model rats. Method: Sprague Dawley rats (Grade:SPF) were randomly divided into 8 groups:normal group, dementia model group, Acori Tatarinowii Rhizoma-Polygalae Radix volatile oil low, middle, high dosage groups. and Acori Tatarinowii Rhizoma-Polygalae Radix aqueous extract low, middle, high dosage groups. Subacute aging models were established by D-galactose (D-gal). After successful modeling, the rats in treatment groups were given with different components of Acori Tatarinowii Rhizoma-Polygalae Radix 0.6, 1.2, 1.8 g · kg-1 (crude drug dosage) by gastric lavage;while the rats in normal group and model group were given with corresponding dose of normal saline. The 28 days later, their ability of spatial learning and memory was tested by Morris water maze in rats, and hippocampal synaptic protein levels in rats of various groups were detected by Western blot. Result: Compared with normal group, the latency period was prolonged in model group (P<0.01). Compared with the model group, the latency period was shortened in drug treatment groups (P<0.01). The latency period in Acori Tatarinowii Rhizoma-Polygalae Radix aqueous extract high dose group was shorter than that in low dose group and all volatile oil groups (P<0.05). In spatial probe test, aqueous extract high dose group was significantly superior to its low dose group (P<0.01) and all volatile oil groups (P<0.05). Both components of Acori Tatarinowii Rhizoma-Polygalae Radix can make the expressions of Synaptophysin, Synapsin1, PSD-95, and PSD-93 in AD model rats higher than those of D-gal model rats (P<0.05), with a dose-dependent relationship, but the Synapsin1 expression of volatile oil was decreased with the increase of dose. Conclusion: Acori Tatarinowii Rhizoma-Polygalae Radix can promote synaptic proteins expression in the hippocampus of AD model rats, especially the aqueous extract component has the greatest effect.关键词：Acori Tatarinowii Radix;Polygalae Radix;volatile oil;aqueous extract;learning and memory capacity;hippocampus;synaptic proteins
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发布时间：2024-01-04 - 摘要：Objective: To observe the hemorrheology and tissue plasminogen activator (t-PA) changes in uterus subinvolution rats after delivery, and explore the construction of uterus subinvolution rat models and intervention mechanism of Yiqi Huayu decoction. Method: Fifty pregnant rats were randomly divided into 5 groups:normal group, model group, Xinshenghua group (5.4 g · kg-1), and Yiqi Huayu low dose group and high-dose group (4.68, 18.72 g · kg-1). Rats in all other groups except normal group received postpartum intrauterine administration of Escherichia coli to prepare inflammation postpartum uterussubinvolutionmodels.All rats were administered by intragastricinfusion with corresponding drugs during day 1-7 after the models were established. HE staining of the uterus tissues was done to observe the morphology of uterus. Blood rheometer was used to detect the hemorrheology index and double antibody sandwich method was used to detect tissue plasminogen activator (t-PA) levels. Result: Compared with the normal group, whole blood viscosity and plasma viscosity were significantly increased in model group (P<0.05) .Compared with model group, whole blood visosity and plasma viscosity were significantly decreased in Xinshenghua group and low-, high-dose Yiqi Huayu groups (P<0.05), and the effect in Yiqi Huayu high-dose group was better than that in Xinshenghua group (P<0.05).The level of t-PA in model group was higher than that in normal group (P<0.05). Levels of t-PA were decreased in Xinshenghua group and Yiqi Huayu low and high dose groups (P<0.01), but there was no statistically significant difference between various treatment groups. Conclusion: Postpartum uterus subinvolution rat models induced by endometritis meet the requirements of blood stasis syndrome animal models.Yiqi Huayu decoction could improve the viscous state of postpartum blood, accelerate blood circulation, and promote postpartum blood discharge.It also could reduce the high levels of t-PA, decrease the blood viscosity degree, inhibit the formation of new blood stasis, promote regeneration of blood, and help postpartum uterine involution.关键词：blood stasis syndrome;uterus subinvolution;Yiqi Huayu decoction;hemorrheology;tissue plasminogen activator (t-PA)
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Duzhong Wan with different proportions on the osteoporosis in ovariectomized rats. Method: Post menopausal osteoporosis (PMOP) rat models were established by bilateral ovariectomy of 3-month-old SD female rats. 4 weeks after successful modeling, 70 model rats were randomly divided into the model group, estrogen group (E2 group), Eucommiae Cortex and Dipsaci Radix (with different proportions) groups (2 : 1, 1 : 1, 1 : 2, 1 : 0, 0 : 1). The rats with only removal of part fat tissues around the ovary in modeling were used as sham operation group. Corresponding drugs and distilled water were given by ig administration to the rats. Bone mineral density (BMD), bone microstructure parameters, serum estradiol (E2), bone morphogenetic protein 2 (BMP-2) and c-terminal peptide of collagen type Ⅰ(CTX-Ⅰ) levels were detected respectively after treatment 12 weeks. Result: Compared with sham operation group, uterine coefficient, right femur coefficient, BMD, bone volume fraction (BV/TV), and serum estrogen levels were significantly decreased in model group, but the level of BMP-2 and CTX-Ⅰin serum were significantly increased (P<0.01, P<0.05). Compared with the model group, uterine coefficient in E2 group and 1 : 1 group, right femur coefficient and BMD in E2 group, 2 : 1 group, 1 : 1 group, 1 : 2 group and 1 : 0 group, as well as BV/TV in E2 group, 2 : 1 group, and 1 : 1 group were significantly increased;serum BMP-1 levels in 2 : 1 group, 1 : 1 group and 1 : 0 group, and serum CTX-I levels in E2 group, 2 : 1 group, 1 : 1 group, 1 : 2 group and 1 : 0 group were significantly decreased (P<0.01, P<0.05). Conclusion: The results suggested that Duzhong Wan with different proportions of Eucommiae Cortex and Dipsaci Radix had treatment effect for post menopausal osteoporosis, while there may be a difference in intensity of the effect.关键词：Duzhong Wan;post menopausal osteoporosis;bone mineral density;bone microstructure
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Dedu Honghua-7 powder and its optimized prescriptions on mRNA expression of type Ⅰ and Ⅲ collagen in rats with liver fibrosis. Method: Forty Wistar rats were randomly divided into normal group, model group, positive group, original drug group and optimized prescription group. The liver fibrosis models were induced by abdominal cavity injection of olive oil solution containing 30% of CCl4.At the same time, drugs were given 1 time/day by intragastrical administration. Rats in positive group received 0.4 mg ·kg-1 of colchicine pill; rats in the original drug group received 0.6 g ·kg-1 of Dedu Honghua-7 powder; rats in optimized prescription group received 0.6 g ·kg-1 of Dedu Honghua-7 powder optimized product. The rats were sacrificed after 40 days of treatment. The livers were picked out for picrosirius red staining to observe fibrosis degree of liver tissues and type Ⅰ, Ⅲ collagen classifications. ELISA assay was applied to detect the levels of type Ⅲ procollagen, type Ⅳ collagen, hyaluronic acid(HA) and laminin(LN) in liver tissue homogenate. The real-time qPCR was used to detect changes in mRNA expression of type Ⅰ collagen and type Ⅲ collagen. Result: In picrosirius red staining, the levels of type Ⅰ and Ⅲ collagenous fibersin model group were higher than those in normal group; the levels of type Ⅰ, Ⅲ collagenous fibersin positive group, original drug group and optimized prescription group were lower than those in model group. In ELISA assay, the levels of type Ⅳ collagen and HA in model group werehigher than those in normal group (P<0.05). The levels of type Ⅲ procollagen, type Ⅳ collagen, and HA contents in positive group, original drug group, and optimized prescription group were lower than those in model group (P<0.05). In real-time qPCR method, mRNA expressions of type Ⅰ and Ⅲ collagen in model group were higher than those in normal group (P<0.01).The mRNA expressions of type Ⅰ and Ⅲ collagen in positive group, original drug group and optimized prescription group were lower rhan those in model group (P<0.01). The mRNA expressions of type Ⅰ collagen in original drug group and optimized prescription group were lower than those in positive group (P<0.05). The mRNA expression of type Ⅰ collagen in optimized prescription group was lower than that in original drug group (P<0.05). Conclusion: Dedu Honghua-7 powder and its optimized prescription can block or delay the occurrence and development of liver fibrosis by inhibiting the mRNA transcription of type Ⅰ, Ⅲ collagen, and the efficacy of optimized prescription is better than that of original powder.关键词：Dedu Honghua-7 powder;liver fibrosis;mRNA expression of type Ⅰ collagen;mRNA expression of type Ⅲ collagen
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Xiaochaihu Tang on the mRNA expression levels of melanogenesis related genes, including tyrosinase (TYR), tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2) in B16 melanoma cells. Method: CCK8 assay was used to test the cell activity, and low dose, middle dose and high dose Xiaochaihu Tang (0.1, 1, 2.5 g · L-1) were used in the test. Reverse transcription-polymerase chain reaction(RT-PCR) was applied to measure the mRNA expression levels of TYR, TRP-1 and TRP-2. Result: Compared with the blank group, Xiaochaihu Tang can significantly down-regulate the mRNA expression levels of TYR, TRP-1 and TRP-2 with the increase of concentrations (P<0.05, P<0.01). Conclusion: Xiaochaihu Tang could significantly suppress melanogenesis not only by inhibiting TYR activity, but also by down-regulating the gene expression of TRP-1 and TRP-2 as well as protein synthesis, indicating that Xiaochaihu Tang could inhibit melanogenesis by regulating pathways after enzyme protein transcription.关键词：Xiaochaihu Tang;melanogenesis;tyrosinase;tyrosinase related protein-1;tyrosinase related protein-2
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Huoxue Qushi formula on the non-alcoholic steatohepatitis (NASH) model rat and explore it possible mechanism. Method: All Wistar rats of NASH model were induced by feeding high fat diet for 8 weeks, and they were randomly divided into the rogridone group(3 mg · kg-1 · d-1), the low, middle, high dose Huoxue Qushi formula groups (8, 16, 32 g · kg-1 · d-1), the model and the normal group. After 4-week intervention, the levels of alanine aminotransferase (ALT), aspartate amino transferase (AST), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) were determined by a colorimetric method. The levels of fasting blood-glucose (FBG) and fasting insulin (FINS) were determined by radioimmunoassay. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL )-10 and IL-6 were determined by ELISA. The activity of superoxide dismutase (SOD) and the content of malonaldehyde (MDA) were determined. The liver pathology was observed by HE staining and peroxisome proliferator-activated receptor α(PPARα) mRNA expression of liver homogenate was tested by RT-PCR. The content and distribution of CYP2E1 were tested by the ABC method. Result: Compared to the normal group, the levels of ALT, AST, TG, TC, FBG, FINS, TNF-α and IL-6 increased, the levels of HDL-C and IL-10 decreased, the SOD activity reduced, the contents of MDA and CYP2E1 increased and the PPARα mRNA expression reduced in the model group (P<0.05). Compared with model group, all the above indexes and the liver pathology were improved in the Huoxue Qushi formula groups (P<0.05). Pathological results showed that the liver tissue injury obviously improved. Conclusion: The Huoxue Qushi formula has certain effect in effectively treating NASH through many ways involved in improving insulin resistance, reducing lipid peroxidation and inhibiting the inflammatory reaction.关键词：Huoxue Qushi formula;high fat purification diet;nonalcoholic fatty liver disease;insulin resistance;lipid peroxidation;inflammation reaction
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of Guishao granules (GG) on hepatic fibrosis induced by carbon tetrachloride (CCl4) in SD rats. Method: In 90 male SD rats, 15 of them were used as the normal group, and other rats were used to induce hepatic fibrosis models by subcutaneously injecting with CCl4(1 mL · kg-1), two times/week for a total of 13 weeks. The rats were randomly divided into model group, GG low dose group, middle dose group, high dose group (3.5, 7.0, 14 g · kg-1) and colchicine group (0.1 mg · kg-1), n=15 in each group. After 13 weeks, liver indexes of the rats were detected;partial liver tissues were taken for hematoxylin-eosin (HE) staining and Masson staining. Blood was taken to measure the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Levels of hyaluronic acid (HA) and laminin (LN) in serum were detected by radioimmunoassay. The levels of hydroxyproline (Hyp) in liver of rats were measured by spectrophotometric method and the expression of α-smooth muscle actin (α-SMA) in liver was detected by immunohistochemistry method. Result: Compared with the normal group, liver injury and hepatic fibrosis were obvious in the rats of model group;the levels of ALT, AST, HA and LN in the serum were significantly increased in the rats with liver fibrosis;the level of Hyp in liver tissues were significantly increased;and the expression of α-SMA protein in liver tissues was significantly increased (P<0.05, P<0.01). GG could significantly improve liver injury of rats, reduce hepatic fibrosis, significantly reduce the levels of ALT, AST, HA, and LN in the serum of model rats, decrease the level of Hyp in liver tissues, and inhibit the expression of α-SMA protein in liver tissues of the model rats(P<0.05, P<0.01). Conclusion: GG could significantly attenuate hepatic fibrosis induced by CCl4 in rats, and its mechanism may be associated with inhibiting the activation of hepatic stellate cells.关键词：Guishao granules;hepatic fibrosis;carbon tetrachloride;protective efficacy
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发布时间：2024-01-04 - 摘要：Objective: To investigate the pharmacological basis of Qi regulating and depression dispersing activities for Citrus changshan-huyou as one of the habitually used varieties of Aurantii Fructus by comparing the pharmacological effects between Citrus changshan-huyou and different sources of Aurantii Fructus. Method: The 90 ICR mice were randomly divided into normal group (normal), Citrus aurantium ‘Huangpi’ group (HP), Citrus changshan-huyou group (CSHY), Citrus aurantium ‘Daidai’ group(DD), Citrus aurantium ‘Chuluan’group (CL), with high dose (2.0 g · kg-1) and low dose (0.6 g · kg-1) for each varieties, nine groups in total, and 10 mice in each group. Mice tracheal phenol red secretion and intestinal carbon propulsion models were established and treated by oral administration. After the modeling, absorbance A of phenol red liquid samples and small intestinal telecarbon propulsion rate were measured. Tracheas and ileums were isolated from adult guinea pig to prepare contraction models of isolated tracheal smooth muscle and contraction models of isolated ileum. The drugs were added in nutrient solution, with high dose (2.50 g · L-1), medium dose (1.25 g · L-1), and low dose(0.50 g · L-1) groups. Effect values were measured for each dose after the modeling. Result: Compared with the normal group, all of Citrus changshan-huyou samples and Aurantii Fructus samples from 3 different sources could improve the secretion of phenol red in trachea of mice significantly, and inhibit the spontaneous contraction of isolated tracheal smooth muscles in guinea pigs significantly (P<0.05), meanwhile, all of the samples of Aurantii Fructus and Citrus changshan-huyou could also significantly improve the small intestinal telecarbon propulsion rate of the normal mice (P<0.05), and inhibit the spontaneous contraction of the isolated ileum in guinea pigs (P<0.05). There were no significant differences between samples of Aurantii Fructus and Citrus changshan-huyou within the same dosage group. Conclusion: Citrus changshan-huyou and 3 different sources of Aurantii Fructus have no significant differences in Qi regulating and depression dispersing functions, providing the pharmacological basis of Qi regulating and depression dispersing activities for citrus changshan huyou as Aurantii Fructus.关键词：Aurantii Fructus;Citrus changshan-huyou;Qi regulating and depression dispersing;comparison of pharmacodynamics difference
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发布时间：2024-01-04 - 摘要：Objective: To find new antibacterial compounds, carry out the chemistry research on insect natural products, and find effective antimicrobial sites of insects through pharmacological experiment. Method: The insect bodies were extracted by two stage extraction solvent. Firstly, the bodies were impregnated two times with dichloromethane solvent, and then, soaked with 70% methanol soaking two times. Drug sensitive test was used to qualitatively detect the drugs and their minimum inhibitory concentrations (MIC) were tested by concentration gradient dilution method. Result: Of all eleven species of insects, only extracts (respectively Periostracum cicadae butanol, Mole cricket ethyl acetate, P.cicadae ethyl acetate and P. cicadae water sites) of P. cicadae and the M. cricket have significant antibacterial activity to Staphylococcus aureus and Mycobacterium tuberculosis, and P.cicadae ethyl acetate has the best antibacterial effect against S.aureus. Conclusion :This study will provide new ideas for the discovery of new antibiotics and insects chemical research for development of new medicines.关键词：insect extracts;antibacterial activity;Periostracum cicadae;Mole cricket
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发布时间：2024-01-04 -
Effect of Lespedeza Bicoloris Radix Extract on Animal Models of Uterine Fibroidand Blood Stasis Rats
摘要：Objective: To explore the effect of Lespedeza Bicoloris Radix extracts (LBE) on animal models of uterine fibroid and blood stasis rats. Method: KM mice of the same birth date and SD rats of the same birth date were divided into normal groups and model groups. Estradiol benzoate was used in modeling animals to induce uterine fibroid rat models and uterine fibroid mice models. The modeling rat groups were randomly divided into model group, Tamoxifen citrate tablets positive group (0.18 g · kg-1) and LBE high dose, middle dose and low dose groups (1.0, 0.5, 0.25 g · kg-1). The modeling mice were randomly divided into model group, Tamoxifen citrate tablets positive group (0.23 g · kg-1) and LBE high dose, middle dose and low dose groups (1.4, 0.7, 0.35 g · kg-1). The rats were randomly divided into normal group, model group, Compound Danshen tablets positive group (0.34 g · kg-1) and LBE high dose, middle dose and low dose groups (1.0, 0.5, 0.25 g · kg-1). All the other rats except normal group received subcutaneous injection of adrenaline hydrochloride in back. 2 h later, rats received dipping bath in ice water to induce acute blood stasis mice models. After continuous drug administration for two weeks, uterus coefficient, uterine dry weight, serum estrogen level, and pathological changes of uterus in uterine fibroid animal models and blood rheology in blood stasis rats were observed. Result: After continuous administration for two weeks, uterus coefficient, uterine dry weight, and serum estrogen levels were decreased in uterine fibroid rats and mice LBE groups, and the pathological changes were alleviated in uterine fibroid mice;whole blood viscosity, plasma viscosity, whole blood high shear relative index, whole blood low shear relative index, and erythrocyte aggregation index were decreased in blood stasis rats (P<0.01, P<0.05). Conclusion: LBE has a certain therapeutic effect on animal models of uterine fibroid.关键词：uterine fibroid;promoting blood circulation to remove blood stasis;Lespedeza Bicoloris Radix- 2
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect and mechanism of hawthorn extract on apoptosis of hepatoma cells HepG2 in vitro. Method: Ethonal fraction was extracted from hawthorn, then HepG2 cells were treated by hawthorn extract at concentrations of 0.1, 0.2, 0.4 and 0.8 g · L-1 for 24, 48 and 72 h. Effect of the extract on hepatoma cells activity was detected by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay;apoptotic rate was measured by flow cytometry after HepG2 cells were treated by hawthorn extract for 48 h. Gene expressions of cysteinyl aspartate specific proteinase (Caspase-3), B-cell lymphoma-2 (Bcl-2), and Bax were detected by real-time PCR;protein expressions of cleaved-caspase3, Bcl-2 and Bax were detected by Western blot. Result: Hawthorn extract significantly inhibited the activity of HepG2 cells, and induced apoptosis in time-and concentration-dependent manners. Bcl-2 expression was inhibited in mRNA and protein level, while Bax and Caspase-3 were up-regulated by extract. Conclusion: Hawthorn extract could inhibit proliferation and promote apoptosis of HepG2 cells, and its mechanism may be associated with increasing expressions of Bax and Cleaved-Caspase-3 and reducing Bcl-2/Bax ratio.关键词：hawthorn;extract;HepG2;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Duhuo Jisheng Tang combined with meloxicam in the treatment of rheumatoid arthritis (RA), and observe its effect on serum interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule (ICMAM-1) levels. Method: The 92 cases of rheumatoid arthritis in our hospital from April 2012 to December 2014 were selected as the research objects, and were divided into two groups according to the random number table method. Patients in control group (45 cases) were treated with meloxicam (15 mg · time-1, 1 time · d-1) by orally taking, and also with symptomatic and supportive treatment. Patients in test group (47 cases) were also given with Duhuo Jisheng Tang based on treatment in control group. Treatment course was 12 weeks for both groups. Clinical symptoms in both groups were observed and recorded before and after treatment, such as morning stiffness time, swelling index, tenderness index, 20 m walking time, two-hand grip and activities of daily living score in Health Assessment Questionnaire (HAQ). In addition, C-reactive protein (CRP) treatment, erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), IL-1, TNF-α and ICMAM-1 levels were detected before and after treatment. Result: According to standards on RA conditions recommended by American College of Rheumatology, total effective rate was 97.9% in test group, significantly higher than 82.2% in control group (P<0.05). After treatment for 12 weeks, duration of morning stiffness, swelling index, tenderness index, 20 m walking time, hand grip and HAQ indexes were significantly improved in both groups, with significant difference when compared with conditions before treatment (P<0.01). Improvement degree in above indexes in test group was more significant than that in control group, with significant difference (P<0.01). Levels of CRP, ESR, RF, IL-1, TNF-α, and ICMAM-1 were significantly decreased in both groups, with significant difference when compared with the conditions before treatment (P<0.01). Decrease degree in above indexes in test group was more significant than that in control group, with significant difference(P<0.01). Conclusion: Duhuo Jisheng Tang combined with meloxicam in the treatment of RA has good clinical effect, significantly improves the clinical symptoms of patients, and has certain inhibitory effect on the body's inflammatory response, worthy of clinical use.关键词：Duhuo Jisheng Tang;meloxicam;rheumatoid arthritis
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发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy and mechanism of Tianzhi granules combined with donepezil for mild-to-moderate vascular dementia patients with liver-yang excessive syndromes by using the nuclear magnetic resonance (NMR) spectrum. Method: A total of 80 mild-to-moderate vascular dementia patients with liver-yang excessive syndrome were randomly divided into 2 groups according to random number table:treatment group and control group, with 40 patients in each group. Patients in control group were given with donepezil orally (5 mg/time, 1 time/d);Patients in treatment group were also given with Tianzhi granules orally (3 times/d, 5 g/time) based on the treatment of control group. Treatment course was 90 days in both groups. Mini-Mental State Examination Scale (MMSE), Activity of Daily Living Scale (ADL) and Gan Yang Shang Kang Scale (GYSK) were evaluated before and after intervention. Multivoxel 1H-MRS examinations were performed with PRESS sequence and GE Signa EXCITE 3.0 T Magnetic Resonance Imaging system was used in the examination. The changes of N-acetyl-aspartic acid/creatine (NAA/Cr), choline/creatine (Cho/Cr) ratios at left frontal lobe and hippocampus were observed as well. Result: Before treatment, no significant difference was observed in dementia degree, MMSE, ADL score and GYSK score between two groups. After 90 days of treatment, both groups had improvement in MMSE and ADL scores (P<0.05).GYSK score was decreased in control group, however the difference was not statistically significant .GYSK score was decreased significantly in treatment group, and it was lower than that of control group, with more significant difference (P<0.05). After 90 days of treatment, Cho/Cr ratio in the left frontal lobe and hippocampus was significantly decreased in both groups, while the NAA/Cr ratio was significantly increased in both groups (P<0.05). After 90 days of treatment, the NAA/Cr ratio of left frontal lobe and hippocampus in treatment group was higher than that of control group with statistical significance (P<0.05), and the Cho/Cr ratio in treatment group was lower than that of control group without statistical significance. Conclusion: The efficacy of Tianzhi granules combined with donepezil therapy was superior than that of donepezil alone, and the mechanism may be associated with promotion of neuron repair, inhibition of gliocyte proliferation and regulatiojn of phospholipid metabolism of nerve cell membrane.关键词：Tianzhi granule;vascular dementia;magnetic resonance spectrum
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Jiangzhi Hugan Tang in treatment of non-alcoholic steatohepatitis (NASH) in different physiques and observe its effect on anti-oxidative stress of liver. Method: One hundred NASH patients were randomly divided into control group (50 cases) and treatment group (50 cases) according to random number table. Patients in two groups were given with non-drug basic treatment referring to Consensus on the diagnosis and management of non-alcoholic fatty liver disease by Integrated Chinese and western medicine. Patients of control group were also treated with diammonium glycyrrhizinate tablets, 150 mg/time, tid. Patients of treatment group were also treated with Jiangzhi Hugan Tang (1 dose/d and bid). The treatment course was 3 months for both groups. Changes in blood lipid level and liver function were compared between the two groups before and after treatment. Changes of clinical symptoms and signs were observed in both groups and the clinical efficacy was retrospectively analyzed as well. Levels of total superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), reduced glutathione (GSH) and malondialdehyde (MDA) in serum were measured in two groups. Result: Levels of triglycerides (TG), total cholesterol (TC) and low density lipoprotein (LDL) were in treatment group were significantly lower than those in control group, while the level of high density lipoprotein (HDL) was significantly higher in treatment group(P<0.01). Levels of alanine aminotransferase (ALT), aspartate transaminase (AST), total bilirubin (TBIL), γ-Glutamine transpeptidase (γ-GT) and scores of Chinese medicine physiques in treatment group were significantly lower than those in control group after treatment (P<0.01). The total efficacy of treatment group was 94%, higher than 76% in control group (P<0.05). Levels of SOD, GSH-Px and GSH in serum of treatment group were significantly higher than those in control group, however MDA level was significantly lower than that of control group, with statistical difference (P<0.01). Conclusion: Jiangzhi Hugan Tang in treatment of NASH by different physiques could protect hepatic function, ameliorate the lipid disturbance and scores of Chinese medicine physiques, and improve clinical efficacy. It is suggested that one of the mechanisms above might be associated with strengthening hepatic capacities of anti-oxidation stress.关键词：non-alcoholic steatohepatitis;Jiangzhi Hugan Tang;oxidation stress;physique
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of Lidan Paishi Tang in expectant treatment of chronic cholecystitis (liver and qi stagnation syndrome type) and observe its effect on levels of leptin (LEP) and tumor necrosis factor-α (TNF-α). Method: One hundred and twenty chronic cholecystitis patients in our hospital were randomly divided into control group (60 cases) and treatment group (60 cases) according to random number table. By subtracting the shedding and exclusion cases, actually 59 patients in control group and 58 patients in treatment group completed this study. By referring to Chinese consensus on the medical diagnosis and treatment of chronic cholecystitis and gallstones, patients of control group in acute attack period were treated with nitroglycerin tablets (0.5 mg sublingually, 1 time/4 hours), promethazine hydrochloride tablets orally (2 tablets/time, bid).Patients with poor digestion were treated with compound azintamide enteric-coated tablets (2 tablets/time, tid).Those with infection were treated with intravenous drip of piperacillin sodium and tazobactam sodium (4.5 g/time), diluted by 5% glucose, bid.Metronidazole tablets orally (3 tablets/d, tid). Patients in treatment group were also treated with Lidan Paishi Tang (1 dose/d, bid) based on the treatment in control group. The treatment course was 4 weeks for both groups. The traditional Chinese medicine (TCM) symptom scores, gallbladder wall thickness and gallbladder contract function in two groups were compared before and after treatment.The levels of LEP and TNF-α in serum were detected in two groups. Result: The TCM symptom scores in treatment group were significantly lower than those in control group after treatment (P<0.01). The total efficacy of treatment group was 93.1%, higher than 76.27% in control group (P<0.05). Compared with control group, gallbladder contract function was significantly higher and gallbladder wall thickness was significantly lower in treatment group after treatment (P<0.01). The levels of LEP and TNF-α in serum of treatment group were significantly lower than those of control group after treatment, with statistically significant difference (P<0.01). Conclusion: Lidan Paishi Tang combined with expectant treatment for chronic cholecystitis with liver and qi stagnation syndrome could improve TCM symptom scores, gallbladder contract function, reduce gallbladder wall thickness and levels of LEP and TNF-α in serum and thus increase clinical efficacy.关键词：Lidan Paishi Tang;chronic cholecystitis;liver and Qi stagnation syndrome;leptin;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To investigate the potential action mechanism and observe the effects of traditional Chinese medicine compound (TCMC) on the expressions of anti-thyroid autoantibodies of Hashimoto's thyroiditis, cluster differentiation antigen 4 (CD4+)T lymphocytes, serum interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α), as well as the pathological changes of thyroid. Method: Seventy patients with Hashimoto's thyroiditis were randomly divided into two groups on average (no significant difference in sex, age and disease course):treatment group and non-treatment group. The patients in treatment group were treated with TCMC (one dose/day and 12 weeks/course), while the patients in non-treatment group were treated with Euthyrox by oral administration. Before and after treatment, free triiodothyronine (FT3), free (unbound) thyroxin (e) (FT4) and thyroid stimulating hormone (TSH) were tested once respectively. CD4+ TC, thyroid globulin antibody (TGAb), thyroid peroxidase antibody (TPOAb), IL-12 and TNF-α as thyroid immunological markers were also tested once respectively before and after treatment;coarse needle biopsy (CNB) was also done once respectively before and after treatment. Result: There was no statistically significant difference in thyroid function between two groups of patients before and after treatment, and there was no significant change in FT3, TSH, and FT4 in treatment group before and after treatment, indicating that Chinese medicine had no significant effect on thyroid hormone levels. Before treatment, there was no statistical difference in TGAb, TPOAb, IL-12, and TNF-α between two groups. CD4+ TC, TGAb, TPOAb, IL-12, TNF-α levels after treatment in treatment group were significantly lower than those before treatment and those in non-treatment group, indicating that this drug can well regulate immunity, reduce thyroid humoral immune damage, and promote tissue repair. Conclusion: This TCMC could mediate body immunity well, relieve the thyroid humoral immunity injury and accelerate the tissue repair, demonstrating protective and repairable function for the thyroid tissues of Hashimoto's thyroiditis.关键词：hashimoto's thyroiditis;CD4+T lymphocyte;interleukin-12;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Zhuyu Huoluo detection combined with botulinum toxin A in treating primary trigeminal neuralgia (PTN) (blood stasis resistance type) and observe its effect on levels of 5-hydroxytryptamine (5-HT), C-reactin protein(CRP) and homocysteic acid (HCY) in serum. Method: One hundred and thirty eligible PTN cases in our hospital were randomly divided into control group and treatment group (65 cases of each group) by random number table. Patients of control group were treated with botulinum toxin A local multipoint injection (2.5-5.0 U/point, 40-150 U/time). Based on the treatment of control group, patients in treatment group were also treated with Zhuyu Huoluo detection. Scores of survival quality and visual analogue scale (VAS) were compared between two groups. Clinical efficacy was analyzed after treatment between both groups. Levels of 5-HT, CRP and HCY in serum were detected in two groups. Result: Scores of survival quality in treatment group were significantly lower than those in control group after treatment (P<0.01). Clinical total effective rate of treatment group was 93.85%, higher than 80% in control group(P<0.05). VAS scores in treatment group were significantly lower than those in control group after treatment of 2 and 4 weeks (P<0.01). Level of 5-HT in serum of treatment group was significantly higher than that of control group after treatment while CRP and HCY levels were significantly lower than those in control group(P<0.01). Conclusion: On the basis of western medicine treatment of botulinum toxin A, Zhuyu Huoluo detection in treating PTN of blood stasis resistance type could obviously improve survival quality, decrease VAS scores and improve clinical efficacy, and its mechanism may be associated with reducing levels of HCY and CRP and increasing 5-HT level in serum.关键词：Zhuyu Huoluo detection;botulinum toxin A;primary trigeminal neuralgia;blood stasis resistance type
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Zhongfeng Fangzhiling capsule on preventing and treating restenosis after stent implantation for carotid artery stenosis. Method: The 60 postoperative patients with stent implantation for carotid artery stenosis were randomly divided into 2 groups, with 30 cases in each group. Patients in control group were treated with aspirin and clopidogrel. Patients in treatment group were also treated with Zhongfeng Fangzhiling capsule by orally taking based on the treatment in control group. Patients in both groups were treated for 180 days. Patients in both groups were observed for restenosis rate, carotid color Doppler ultrasound, transcranial doppler (TCD) detection, thromboxane A2(TXA2), prostaglandin I2 (PGI2), and blood rheology changes. Result: Total effective rate was 90.0% by combined Chinese and western medicine in treatment group, higher than 73.3% in control group(P<0.05). The stenosis rate in lesion of control group higher than in treatment group(P<0.05). The clinical efficacy, TCM symptom scores, and scores of wind-phlegm stasis syndrome in Zhongfeng Fangzhiling capsule combined with western medicine group were significantly superior to those in western medicine alone group. In addition, Zhongfeng Fangzhiling capsule combined with western medicine could significantly reduce the unstable plaque of internal carotid, plaque length, plaque thickness, improve blood rheology, lower the TXA2 level, increase the level of PGI2, and can reduce the incidence of recent bleeding (complication), with better effect than western medicine alone. One year after the implantation, no transient ischemic attack (TIA) or cerebral infarction was found in patients in the clinical follow-up. Conclusion: Zhongfeng Fangzhiling capsule combined with western medicine has better efficacy in preventing and treating restenosis after stent implantation for carotid artery stenosis, and can reduce the incidence of recent bleeding (complication).关键词：carotid artery stenosis;restenosis after stent implantation;Zhongfeng Fangzhiling capsule
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Qingzhu detection on cortisol(CS) levels in serum during treatment of adult onset Still's disease(AOSD) (Qi and Yin deficiency and damp-heat blockage type). Method: Totally 60 eligible patients with AOSD from October 2013 to June 2015 were randomly divided into test group (n=30) and control group (n=30). Qingzhu detection, bid for two weeks as a period of treatment was given to 30 patients in test group and diclofenac sodium sustained release tablets (150 mg per day, 75 mg · time-1, bid) were given to 30 patients in control group. The treatment course was 2 weeks for both groups. Scores of symptoms and signs were recorded and CS levels in serum were detected before treatment and after treatment. At week 4 and week 8 after treatment, scores of symptoms and signs were also recorded in both test group and control group. Result: Scores of symptoms and signs were significantly improved after treatment in both groups (P<0.01), and scores in test group were obviously superior to those in control group (P<0.05).CS level in serum was increased in patients of test group after treatment (P<0.05). Improvement degree of symptoms and signs was positively correlated with CS level in serum (r=0.609), and improvement degree of symptoms had dependence on CS level in serum (P<0.05). According to the follow-up records of symptom and sign scores at week 4 after treatment, test group was significantly superior to control group (P<0.01). Conclusion: Qingzhu detection can effectively control AOSD by improving the level of CS in serum.关键词：adult onset Still's disease;cortisol;Qingzhu detection
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of Shenmai injection for myocardial damage caused by chemotherapy in postoperative breast cancer patients, and observe its effect on oxidative stress. Method: The 98 postoperative breast cancer patients were randomly divided into control group (49 cases) and treatment group (49 cases) with reference to digital table method. Patients of control group were treated with CEF chemotherapy [cytoxan (CTX) 75 mg ·m-2+epirubicin(EPI) 60 mg ·m-2+fluorouracil 500 mg ·m-2], with 21 days as one cycle, one time/cycle, for a total of 6 cycles. Patients of treatment group were also treated with Shenmai injection based on the treatment in control group, 60 mL/time, by intravenous drip of 500 mL 5% glucose injection, from 1 d before chemotherapy to one week after chemotherapy, 7 times/cycle. The traditional Chinese medicine (TCM) symptom scores and cardiac function pre-post treatment were compared between two groups. Levels of cardiac troponin-I(cTnI), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), total superoxide dismutase (SOD), reduced glutathione (GSH) and malondialdehyde (MDA) were detected in two groups. Result: At the ending of chemotherapy and 1 week after chemotherapy, the TCM symptom scores in treatment were significantly lower than those in control group (P<0.01);cardiac function indexes of E/A and LVEF were significantly higher than those in control group (P<0.01), levels of cTnI, CK, CK-MB, and MDA in treatment group were significantly lower than those in control group, while levels of SOD and GSH in treatment group were significantly higher than those in control group, with statistically significant difference (P<0.01). Conclusion: Shenmai injection has obvious protection effect on myocardial damage caused by chemotherapy in postoperative breast cancer patients, and its mechanism may be associated with improving oxidative stress state.关键词：Shenmai injection;breast cancer;chemotherapy;myocardial damage
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发布时间：2024-01-04 - 摘要：Objective: To discover the knowledge of Glycyrrhizae Radix et Rhizoma dose-response in Treatise on Exogenous Febrile Disease based on the group structure diagram of structural partial-ordered attribute. Method: Formulas and prescriptions about Glycyrrhizae Radix et Rhizoma in the Treatise on Exogenous Febrile Disease were collected to standardize the expression, establish a database, build dose-response group structure diagram of Glycyrrhizae Radix et Rhizoma, and discover the pattern from the diagram. Then the scientific connotation of Glycyrrhizae Radix et Rhizoma dose-response relationship in Treatise on Exogenous Febrile Disease was collected and studied. Result: The dose-response group structure diagram of Glycyrrhizae Radix et Rhizoma could systemically, comprehensively multi-levelly demonstrate the dose-response rules of Glycyrrhizae Radix et Rhizoma. Conclusion: It was concluded that, valuable knowledge on dose-response relationship can be dig outwith the knowledge discoverymethod based on the theory of structural partial-ordered attribute diagram, providing an applicable method for inheritance and development of standardization, systematization, scientization and modernization.关键词：theory of structural partial-ordered attribute diagram;formuladose-response group structure diagram;dose-response relationship;Treatise on Exogenous Febrile Disease;Glycyrrhizae Radix et Rhizoma
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发布时间：2024-01-04 - 摘要：Objective: To systemically evaluate the efficacy and the safety of modified Shashen Maidong Tang combined with chemotherapy in treating patients with non-small cell lung cancer. Method: We searched randomized controlled clinical studies on modified Shashen Maidong Tang combined with chemotherapy for non-small cell lung cancer in the databases of CNKI, Wanfang Data, VIP, CBM and PubMed from the database building date to 2015.Articles were selected in accordance with the inclusion/exclusion criteria;quality of included studies was evaluated and data was extracted, then Revmen5.3 software was used for Meta-analysis. Result: The 10 randomized controlled trials were included for systematic review, all of which were of low quality studies with Jadad score<3.Meta results showed that:compared with the control group, the effective rate [OR= 1.70, 95%CI (1.25, 2.31), P=0.000 7] and stable rate [OR=2.26, 95%CI (1.51, 3.37), P<0.000 1] of Shashen Maidong Tang combined with chemotherapy for non-small cell lung were higher, and it could reduce bone marrow suppression [OR=0.42, 95%CI (0.23, 0.78), P=0.006], with statistically significant difference. In respect of reducing liver function [OR=0.67, 95%CI (0.38, 1.20), P=0.18], renal impairment [OR=0.77, 95%CI (0.38, 1.57), P=0.48] and gastrointestinal symptoms [OR=0.61, 95%CI (0.34, 1.12), P=0.11], there was no significant difference between Shashen Maidong Tang combined with chemotherapy group and control group. Conclusion: Shashen Maidong Tang combined with chemotherapy for non-small cell lung cancer have better efficacy and could reduce side effects of chemotherapy such as marrow suppression. However, the existing research evidence level is low, and more large-sample, high-quality, and multi-center randomized controlled trials are still needed for further verification.关键词：Shashen Maidong tang;chemotherapy;non-small cell lung cancer;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To study the screening methods of core herbal combinations of anti-Alzheimer prescriptions by using Traditional Chinese Medicine Inheritance System (TCMIS). Method: The traditional medical prescriptions for anti-Alzheimer in Dictionary of Chinese Medicine Prescriptions (DCMP) were selected and TCMIS V2.5 was used to input the prescription information and construct the database. The commonly used herbs, combination principles, Dominance & Recessive herb pairs and the correlations between prescriptions and symptoms were analyzed and new prescriptions were developed with data mining methods such as association rules, improved mutual information method and complex system entropy clustering. Result: The 30 commonly used herbs were concluded based on the statistic frequency analysis of 102 anti-Alzheimer prescriptions. Ginseng Radix et Rhizoma, Polygalae Radix, Acori Poria and Tatarinowii Rhizoma ranked top four in frequency. The 20 commonly used herb pairs were concluded based on the association rules analysis of 102 anti-Alzheimer prescriptions. Ginseng Radix et Rhizoma-Polygalae Radix, Polygalae Radix-Tatarinowii Rhizoma, and Ginseng Radix et Rhizoma and Tatarinowii Rhizoma ranked top three among the commonly used herb pairs. In accordance with the correlations between prescriptions and symptoms, the core herbal combinations for anti-Alzheimer prescriptions in DCMP were greatly correlated with Ginseng Radix et Rhizoma, Polygalae Radix, Tatarinowii Rhizoma and Acori Poria. The 40 new core combinations and 2 newly developed prescriptions were concluded based on improved mutual information method and complex system entropy clustering in 102 prescriptions for anti-Alzheimer. Conclusion: The ingredients in core combinations of anti-Alzheimer prescriptions were highly coincided with those in Kaixin San in DCMP, with clear treatment significance for memory loss. The two newly developed prescriptions for anti-Alzheimer found by TCMIS V2.5 have some clinical value.关键词：Dictionary of Chinese Medicine Prescription;Alzheimer disease;Traditional Chinese Medicine Inheritance System V2.5;Kaixin San
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发布时间：2024-01-04 - 摘要：Triptergium wilfordii (TW) is a characteristic traditional Chinese medicine (TCM) in China, with certain effect for autoimmune hyperthyroidism diseases like rheumatoid arthritis (RA). In recent years, it has been one of the hot natural medicines both at home and abroad for functions of anti-inflammatory, anti-tumor, immunomodulatory, anti-fertility, anti-HIV virus and treatment of Alzheimer's disease. However, multiple-organ injury is common in the clinical application of TW, especially the frequent renal toxicity and serious consequences of poisoning. Therefore, application and promotion of TW are restricted. In this paper, we would explore the prescription rules of TW based on the previous researchers' text mining experience, explore and summarize the rules of TCM prescriptions through in-depth analysis and classification. Meanwhile, we would put forward the combination principles of detoxicity prescriptions on renal toxicity of TW compatibility based on "toxicity restriction by strengthening the body resistance" methods under the guidance of the theory of "diversity restriction". For the clinical toxicity and adverse effect of TW, right medicines were selected for compatibility with TW for detoxicity, in order to make them play a comprehensive role in the complementary and opposite phase system with TW, and provide references for improving the security of the clinical application of TW.关键词：Triptergium wilfordii;toxicity restriction by strengthening the body resistance;kidney toxicity;detoxicity by compatibility
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