最新刊期
卷 15 ， 期 11 ， 2009
- 摘要：Objective:To determine of hesperidin in pericarpium citri reticulatae from the different producing area to estimate the quality of Pericarpium Citri Rcti culatae.Methods: The HPLC was adopted,the separation was performed on a Kromasil C18 column（250 mm×4.6 mm,5 μm）,methanol-acetic acid-water（35∶4∶61） was used as the mobile phase,the detective wavelength was at 283 nm,the flow rate was at 1.0 mL·min-1,and the column temperature was at room temperature.Results: The hesperidin showed a good linearity at the range of 0.4～3.6 μg,r=（0.999 8,） the average recovery was 99.28%,RSD=1.09%（n=6）.The contents of hesperidin in the samples collected from different producing area were varied.Conclusion: The method can be used to estimate the quality of pericarpium citri reticulatae.关键词：Pericarpium citri reticulatae;hesperidin;HPLC
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发布时间：2024-09-23 - 摘要：目的:建立以苯酚-硫酸法测定清开灵注射液中总多糖含量的方法。方法:以苯酚-硫酸法显色,以葡萄糖为对照品,测定清开灵注射液中总多糖的含量。结果:葡萄糖的检测浓度在5.037125.9μg.mL-1范围内与吸光度呈良好的线性关系（r=0.9984）;平均回收率为100.02%,RSD=2.30%（n=6）。结论:该方法简单、准确、重复性好,可用于清开灵注射液中总多糖的含量测定。关键词：清开灵注射液;总多糖;苯酚-硫酸法;含量测定
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- 585
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of different concentration of macroelement,microelement and organic element on the growth and accumulation of tanshinones in hairy root of Salvia miltiorrhiza.Method: Hairy root of Salvia miltiorrhiza were cultured by liquid 6,7-V mediums with default,1/8,1/4,normal and 4,8 times of macroelement、microelement and organic element.We determined the growth and the contents of dihydrotanshinone、cryptotanshinone、tanshinoneⅠ、tanshinoneⅡA by high performance liquid chromatography（HPLC） method at the same cultivation period.Result: Macroelement affect the growth and concentration of tanshinones significantly.Default macroelement can promote the accumulation of tanshinones significantly,but hold-back the growth of hairy hoot significantly.High concentration of macroelement can promote the growth of hairy root but reduced the accumulation of tanshinones significantly.Microelement and organic element can not affect the growth and accumulation of tanshinones significantly.Conclusion: Macroelement is the main factor to affect the growth and the accumulation of tanshinones in Salvia miltiorrhiza.Low concentration of macroelement can promote the accumulation of tanshinones,which can supply some foundations to study the cultivation of hairy roots and formation mechanism of the quality of different Salvia miltiorrhiza.关键词：Salvia miltiorrhiza;hairy roots;tanshinones;macroelement;microelement;organic element
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发布时间：2024-09-23 - 摘要：目的:探寻提高六味地黄口服液质量标准的研究。方法:采用HPLC法以十八烷基硅烷键合硅胶为固定相。马钱苷测定,流动相为四氢呋喃-乙腈-甲醇-0.05%磷酸溶液（1∶8∶4∶87）;流速为1 mL.min-1,检测波长为236 nm;丹皮酚的测定,以甲醇-水（70∶30）为流动相,流速为1 mL.min-1,检测波长为274 nm。结果:马钱苷和丹皮酚的线性范围分别为0.041 70.458 9μg、0.043 70.480 5μg;平均加样回收率,马钱苷为:99.47%,RSD:0.45%（n=6）,丹皮酚为99.61%,RSD为1.32%。（n=6）。结论:实验表明,该方法方便快捷,准确,灵敏度高,重复性好,可用于六味地黄口服液的质量控制。关键词：六味地黄口服液;质量标准;高效液相色谱法;马钱苷;丹皮酚
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发布时间：2024-09-23 - 摘要：Objective:To study the HPLC fingerprint of General Ginsenoside in extract of Ginseng radix.Methods: HPLC with Hypersil-C18（4.6 mm×250 mm,5 μm）column was used,was consistea of acetonitril and water with 0.1% H3PO4.The moblie phase and detection wavelength was at 203 nm.The flow rate was 1.3 mL·min-1 and the column temperature was 35 ℃.Results:10 peaks were obsened on the HPLC fingerprint of General Ginsenoside in extract of Ginseng radix.Conclusion:The method can be used for the quality control of General Ginsenoside in the extract of Ginseng radix.关键词：general ginsenoside;fingerprint;HPLC
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发布时间：2024-09-23 - 摘要：目的:建立叶下珠的薄层鉴别及其多酚类成分的含量测定方法。方法:采用聚酰胺薄膜色谱法鉴别叶下珠药材,采用紫外可见分光光度法测定叶下珠中多酚类成分的含量。结果:供试品色谱中,在与没食子酸对照品色谱相应的位置上显相同的蓝色斑点;没食子酸在40.2160.8μg的范围内与吸光度呈良好的线性关系（r=0.9997,n=5）,平均回收率为96.8%,RSD为0.7%。结论:该文建立的鉴别方法快速、简便,专属性强;含量测定方法准确、灵敏,重复性好。关键词：叶下珠;薄层鉴别;多酚类成分;含量测定
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- 338
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发布时间：2024-09-23 - 摘要：目的:完善千斤脑康宁胶囊的质量标准。方法:采用薄层色谱法分别鉴别千斤脑康宁胶囊中的千斤拔、扶芳藤、石菖蒲、丹参、山茱萸。结果:各供试品色谱中,在与对照品及对照药材色谱相应的位置上显相同颜色的斑点,色谱清晰,附近无杂质斑点干扰。结论:该方法专属性强,可更好地控制制剂质量。关键词：薄层色谱法;千斤脑康宁胶囊;千斤拔;扶芳藤;石菖蒲;丹参;山茱萸
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发布时间：2024-09-23 - 摘要：目的:建立康乐洗液中蛇床子素的含量测定方法。方法:高效液相色谱法。采用Symmetry C18柱（3.9 mm×150mm,5μm）,流动相为乙腈-水（65∶35）,检测波长为322 nm。结果:此方法线性关系良好,平均加样回收率为99.88%。结论:此方法简便、准确,可用于测定康乐洗液中蛇床子素的含量。关键词：HPLC;康乐洗液;蛇床子素
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发布时间：2024-09-23 - 摘要：目的:建立八正颗粒质量标准。方法:用薄层色谱法对处方中的大黄、栀子、甘草、车前子进行定性鉴别;用高效液相色谱法对样品中的栀子苷进行含量测定。结果:薄层鉴别的色谱斑点清晰,阴性对照无干扰;含量测定栀子苷进样量在0.089460.53768μg范围内呈良好的线性关系,平均回收率为99.6%（n=5）,RSD为1.47%。结论:定性、定量方法简便、专属、准确,可用于八正颗粒的质量控制标准。关键词：八正颗粒;栀子苷;薄层色谱法;高效液相色谱法
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发布时间：2024-09-23 - 摘要：Objective:To develop a headspace gas chromatography method for the residual organic solvents in butorphanol tartrate.Methods: The sample was injected into ZB-1 capillary column by headspace sampler and analysed with FID detector using ethylacetate as the internal standard and H2O as the solvent.Results: Methanol,acetone,dichloromethane,ether,chloroform,tetrahydrofuran were linear at the ranges of 1.811～394.6 μg·mL-1,1.015～389.41,0.130～407.2,0.447～198.3.776～100.6,0.341～201.8 μg·mL-1,respectively;The recoveries were 97.2%,98.8%,102.5%,102.3%,100.3%,96.4% respectively.Each of the relative standard deviations was no more than 5.0%.Conclusion: The method is simple,accurate and sensitive with good reproducibility.It can be used for the determination of the residual organic solvents in butorphanol tartrate.关键词：butorphanol tartrate;headspace injection;capillary gas chromatography;residual organic solvents determination
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发布时间：2024-09-23 - 摘要：Objective:To develop a method for the determination of harpagide and 8-O-acetylharpagide in Herbal Ajugae.Methods:The determination was performed on Kromasil-C18（250 mm×4.6 mm,5 μm）and detected at 202 nm,the mobile phase was consisted of acetonitrile and water,gradient elution,the flow-rate was at 1.0 mL·min-1.Results:The calibration curves were linear in the range of 0.44 μg to 4.40 μg（r=（0.999 4）） for harpagide and 0.1 μg to 10 μg（r=（0.999 8）） for.The average recovery of harpagide was 100.93% with RSD 1.93%,and 8-O-acetylharpagide was 99.81% with RSD 1.62%.Conclusions: The method is simple,accurate,reproducible.It can be used for the quality control of Ajuga decumbens Thunb.关键词：RP-HPLC;Herbal Ajugae;harpagide;8-O-acetylharpagide;content determination
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- 255
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发布时间：2024-09-23 - 摘要：目的:建立仙乐雄胶囊中淫羊藿的质量控制方法。方法:采用Shim-pack VP-ODS C18反相色谱柱（4.6 mm×150mm,5μm）,以乙腈-水（28∶72）为流动相;流速为1.0 mL.min-1;检测波长270 nm;柱温:30℃。结果:淫羊藿苷在0.1031.858μg范围内线性关系良好（r=0.9999,n=6）,平均加样回收率为98.0%,RSD为1.4%（n=6）。结论:本方法操作简便,结果准确、可靠,可用于仙乐雄胶囊的质量控制。关键词：仙乐雄胶囊;淫羊藿苷;质量控制
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发布时间：2024-09-23 - 摘要：目的:通过研究分析不同产地野菊花挥发油的化学成分,为野菊花的开发利用提供一定实验依据。方法:采用水蒸气蒸馏法提取野菊花挥发油,用GC毛细管柱进行分离,归一化法测其相对含量,并用气相色谱-质谱法对其化学成分进行鉴定。结果:湖北、河北、河南3个产地野菊花含主要成分分别为32个,32个,37个（仅限于已鉴定出的化合物）。结论:不同产地野菊花挥发油化学成分种类和含量均有明显的差别。关键词：野菊花;挥发油;气相色谱-质谱;毛细管柱
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发布时间：2024-09-23 -
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- 摘要：Objective:To study the optimum extraction process of the total flavonoids and quercitrin from cacumen platycladi.Methods: The extract conditions,such as concentration of ethanol,extraction times,extraction time and ratio of solvent to material were investigated by the orthonogal design with the content of tiliroside as the chemical reference.Results: The optimum extraction condition was established as follows: ethanol concentration 70%,extracting 1.5 hours for three times and the ratio of solvent to material 1∶8.Conclusion: The present investigation provided the optimum extraction process of the total flavonoids and quercitrin from the Platycladus orientalis Franco.关键词：platycladus orientalis franco;the total flavonoids;quercitrin;orthogonal design
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- 554
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发布时间：2024-09-23 - 摘要：目的:优选乳核散结片的工艺参数。方法:采用正交试验对当归提取的加水量、温浸时间及蒸馏时间进行了探讨。结果:确定当归提取挥发油的工艺为:加水量为12倍,蒸馏时间为5 h。当归药渣和其余9味用水煎煮2次,每次2 h,故认为12、10倍量水最佳提取条件。结论:最佳提取工艺简便,可行。关键词：乳核散结片;制备;工艺参数;正交试验
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of carbonized cortex moutan on platelet aggregation and TXB2、6-keto-PGF1α in rats.Methods:SD rats were used for the research.The effects of carbonized cortex moutan and the main hemostasia active fractions of carbonized cortex moutan on platelet aggregation induced by ADP and collagen,TXB2 and 6-keto-PGF1α in rats were obserred.Results:Carbonized cortex moutan and the main hemostasia active fractions of carbonized cortex moutan increased platelet aggregation induced by ADP and collagen,then increased the level of TXB2 and decreased the level of 6-keto-PGF1α.Conclusion:The main hemostasia mechanism of carbonized cortex moutan and the main hemostasia active fractions of carbonized cortex moutan is to increase platelet aggregation by affecting the level of TXB2 and 6-keto-PGF1α.关键词：carbonized cortex moutan;platelet aggregation;TXB2;6-Keto-PGF1α
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发布时间：2024-09-23 - 摘要：Objective: Using the method of statistical analysis to explore the relationship between the TCM formulae of different proportions and the treatment for diseases.Methods:We retrieved database containing（1 242） formulae of Radix Angelica and Rhizoma Ligusticum from the "Dictionary of Traditional Chinese Medicine Formulae",and then built the database to analyze the relationship among its ratio,corresponding major disease and its department.Results: The ratio "1∶1" appears to be the most（68.06%）in the（1 242） formulae containing Radix Angelica and Rhizoma Ligusticum;which were most-frequently used for the treatment of the diseases in internal medicine and gynecology.Conclusion:Using the method of the statistical frequency analysis We can find the applied pattern of the TCM formulae and provide a theoretical basis for using herbs effectively in clinic.关键词：Radix Angelicae Sinensis;Rhizoma ligusticum;herbal pair;ratio;data analysis
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发布时间：2024-09-23 - 摘要：Objective:Changes of protein expression of MMP-9/13 and TIMP1/2 in fibrotic liver tissue induced by porcine serum and effect of Xiayuxue Decoction on it are investigated in this study to explore mechanism of Xiayuxue Decotion in reversing it.Methods:Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.After 8wk of modeling,rats were given Xiayuxue Decoction for 4 weeks.Rats were sacrificed and sample was obtained for determination at 1 wk,2 wk,4 wk,8 wk and the end of 12 wk of modeling for determination.Results:High activity of MMP-2/9 and high expression TIMP-1 characterizes this liver fibrosis model from beginning to the end.Decreased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 affect by protein expression TIMP-1/2.Xiayuxue Decoction can increase activity of MMP-9,promote expression of MMP-9/13,inhibit expression of TIMP-1/2.Conclusions:Imbalanced MMP-9/13 and TIMP-1/2 expression is seen in this model.The anti-liver fibrosis mechanism of Xiayuxue Decoction is regulating MMP-9/13 and TIMP-1/2 expression to degrade excessive deposition of extracellular matrix.关键词：liver fibrosis;porcine serum;matrix metalloproteinase;tissue inhibitor of metalloproteinase;extracellular matrix
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发布时间：2024-09-23 - 摘要：Objective:To observe influence of White Prepared Lateral Root of Aconite on cardiac enzymes in normal and KidneyYang insufficiency rats and explore the difference of both.Method:Eighty Sprague-Dawley（SD）male rats were divided randomly into normal group and Kidney-Yang insufficiency model group,forty rats in each group.After producing Kidney-Yang insufficiency model by injecting hydrocortisoni natrii succinas in intramuscular,normal and model rats were respectively and randomly divided into little,low,medium,great dose group and blank group,eight rats in each group.Those rats were administered orally with different concentrations of white prepared lateral root of aconite every day for two weeks.After twenty-four hour the last oral administration,blood serum and cardiac muscle tissue homogenate were prepared and used for testing creatine kinase（CK）,lactate dehydrogenase（LDH）,cardiac muscle succinate dehydrogenase（SDH）,Na+-k+ ATPase,adrenocorticotrophic hormone（ACTH）,hydrocortisone（COR）,cyclic adenosine monophosphate（cAMP）and cyclic guanosine monophosphate（cGMP）.Results:Hydrogenation cortisone caused cAMP and COR descend,but cGMP increase.With adding dose,all rats LDH squinted towards going up,normal rats’LDH going up was quicker than model rats’,which indicated mainly abnormal influence on surrounding cardiac muscle enzyme.However,all rats SDH and Na+-K+-ATPase had a trend of descending.Normal rats’ SDH descending was quicker than model rats’,which showed white prepared lateral root of aconite also impacted rats’cardiac muscle mitochondrion enzyme.Conclusion: White prepared lateral root of aconite had less cardiac toxicity on Kidney-Yang insufficiency rats.This study provided objective evidence for the theory of evaluating traditional Chinese herbal toxicity based on animal model with a specific TCM syndrome.关键词：white prepared lateral root of aconite;Kidney-Yang insufficiency rats;cardiac enzymes
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发布时间：2024-09-23 - 摘要：Objective:To investigate the pharmacological effects of Huanglianjiedu decoction（HLJD） on infectious diseases.Methods:The rabbits febris model was induced by typhoid-paratyphoid A-paratyphoid B triple vaccine,and the effects of HLJD on rabbits’ body temperature was observed;The influence of HLJD to death rate of mice infected by staphylococcus aureus was observed.We also studied the influence of HLJD to dimethyl benzene-induced ear-swelling of mice.Results:HLJD degraded the rabbits’ body temperature obviously,it could protect the staphylococcus aureus-infected mice,decreased the death rate of mice,at the same time,it obviously inhibited the mice ear-swelling.Conclusions:HLJD showed significant relieve fever,antibiosis and anti-inflammatory effects,these provided certain experimental evidence for HLJD in dealing with infectious diseases.关键词：Huanglianjiedu decoction;relieve fever;antibiosis;anti-inflammation
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发布时间：2024-09-23 - 摘要：Objective:To observe and evaluate the anti-tumor effect of MC002,the semi synthesis derivative of triptolide,on human colonic tumor in HCT-8 mice.Methods: BALB/c nude mice of human colonic tumor model was made through HCT-8 cells inoculation.Anti-tumor activity of MC002 was evaluated in terms of relative tumor growth rate,tumor weight and tumor inhibitory ratio in mice transplanted.Results: Tumor volume and weight in every MC002 dosage group were obviously smaller than that in the control group.after ten times of administration,the values of T/C% in the groups of 1.5 mg·kg-1 and 1.0 mg·kg-1 were less than 40% with dose-effect relation ship.Conclusion:MC002 has significant restraining effect on the growth of HCT-8 tumor in mice.It may be developed into a new promising anticancer drug through further studies.关键词：the semi-synthesis derivative of triptolide;anti-tumor activity;human colonic tumor cells;mice with transplanted tumor cells
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- 240
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发布时间：2024-09-23 - 摘要：目的:观察四味降脂汤对肥胖大鼠血清神经肽Y（NPY）含量和下丘脑NPY表达的影响,探讨四味降脂汤减肥的机制。方法:用高脂饲料喂养制作肥胖大鼠模型,将造模成功的实验性肥胖大鼠随机分为模型组和中药组加以治疗。放射免疫法检测血清NPY含量,免疫组织化学法检测NPY表达。结果:四味降脂汤组血清NPY含量和下丘脑NPY表达比模型组降低（P<0.01）;血清NPY水平与普食组比较仍有一定的差异（P<0.01）。结论:肥胖大鼠NPY含量的改善是四味降脂汤减肥的作用机制之一。关键词：肥胖;四味降脂汤;神经肽Y
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of drugs of invigorating spleen,heat-clearing,eliminating stasis on QOUH in rat model of gastric ulcer.Methods:The rat model of gastric ulcer was constructed by injection of acetic acid.The rats were randomly divided into model group,ranitidine group,invigorating spleen group,heating-clearing group,eliminating stasis group,complex prescription group and normal control group.The ulcer index（UI）,regenerated mucosal thickness,lamina muscularis mucosal width and capsular broaden glandular organ quantity were observed by HE,and the levels of EGF and 6-keto-PGF1α were detected by ELISA.The expression of EGFR in the mucosa around the ulcer was detected by SABC kit immunohistochemical method.Results:Compared with Model group,the regenerated mucosal thickness,lamina muscularis mucosae defect extent,UI and capsular broaden glandular organ quantity in invigoraing spleen,heat-clearing,elimianting stasis and complex prescription treating groups were different significantly（P<0.01）.And the effect of complex prescription was better than ranitidine in different degree（P<0.05,or P<0.01）.Compared with ranitidine,invigorating spleen group,heating-clearing group,eliminating stasis increased the levels of 6-keto-PGF1α closed to the normal.Compared with model group and normal control group,the levels of EGF in serum in treating groups were increased significantly（P<0.01）.Positive expression of EGFR around ulcerative tissues in treating groups were strengthened obviously（P<0.05,or P<0.01）,and the effect of complex prescription was the best（P<0.01）.Conclusion:Drugs of invigorating spleen,heat-clearing and eliminating stasis can improve the QOUH and prevent from recurring by improving histological maturation and functional maturation of regenerative mucosa in GU rats.关键词：drugs of invigorating spleen,heat-clearing and eliminating stasis;gastric ulcer;QOUH
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发布时间：2024-09-23 - 摘要：Objective:Objective:To observe the effects and dose-effect relationship of "ethanol extract from Polygonatum Odoratum" on Hemodynamics in Rats of Heart Failure Following Myocardial Infarction.Methods:Seventy healthy Sprague-Dawley rats were divived randomly into sham group,model group,large dosage of "ethanol extract from Polygonatum Odoratum" group,moderate dosage of "ethanol extract from Polygonatum Odoratum" group,small dosage of "ethanol extract from Polygonatum Odoratum" group.After successful model duplication,rats in different dosage of "ethanol extract from Polygonatum Odoratum" groups were treated with intravenous injection of 6 g/kg,3 g/kg,0.3 g/kg distinctly,while rats in sham group or model group received 6 ml/kg saline.The heart rate（HR）,left ventricular systolic pressure（LVSP）,left ventricular diastolic pressure（LVDP）,maximum rise speed of left ventricular pressure（+dp/dtmax）,maximum drop speed of left ventricular pressure（-dp/dtmax）were measured at different time points（before injection and 5,10,20,30 minutes after injection）and comparatively analyed.Result:The HR decreased at 5 minutes in large dosage group and model group,the LVSP was lower,the +dp/dtmax and-dp/dtmax were slower,the LVDP increased at the same time,all these parameter went back to normal at 20 and 30 minutes points,the small dosage group was found changes in these parameters at 10 minutes point except HR.Conclusion:"ethanol extract from Polygonatum Odoratum" has negative inotropic and negative chronotropic action in CHF rats.These two actions have dose-effect relationship.关键词：ethanol extract from Polygonatum Odoratum;myocardial infarction;chronic heart failure;hemodynamics;dose-effect relationship
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发布时间：2024-09-23 - 摘要：目的:研究复方破茎松萝镇痛及对子宫平滑肌的作用。方法:运用醋酸与催产素致痛扭体法研究复方破茎松萝的镇痛作用;运用离体和在体试验研究复方破茎松萝对大鼠子宫收缩活动的影响。结果:复方破茎松萝对醋酸致小鼠扭体反应有一定的镇痛作用,对催产素所致小鼠子宫剧烈收缩引起的疼痛有显著的缓解作用;对大鼠子宫平滑肌（离体和在体）的自发性活动（收缩幅度与收缩频率）有明显的兴奋作用（P<0.05）。结论:复方破茎松萝具有一定的镇痛作用、对大鼠子宫平滑肌有显著的兴奋作用。关键词：复方破茎松萝;镇痛作用;子宫收缩
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of Hanshibi Pellet（HSB） on synovial membrane hyperplasia and expression of MMP-1 and MMP-3 mRNA in rheumatiod arthritis induced by heat-killed mycobacterium tuberculosis H37Ra（Mtb） in SD rats.Methods: The adjuvant-induced rheumatoid arthritis（AA） in SD rats was established by sc immunization with the heat-killed mycobacterium tuberculosis H37Ra（Mtb）.The expression of MMP-1 and MMP-3 mRNA in synovial membrane was measured by using nucleic acid hybridization in situ.Results:HSB could significantly inhibit the injury in ankle joint and decrease the expression of MMP-1 and MMP-3 mRNA in synovial membrane of AA model rats.Conclusions: HSB can inhibit the injury in ankle joint,which is concerned with the decrease of MMP-1 and MMP-3 mRNA expression in synovial membrane of AA model rats.关键词：Hanshibi Pellet;rheumatoid arthritis;matrix metalloproteinase
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发布时间：2024-09-23 - 摘要：目的:观察降脂益肝冲剂对非酒精性脂肪肝（NAFLD）大鼠肝脏肝型脂肪酸结合蛋白（L-FABP）mRNA表达的影响。方法:60只雄性Wistar大鼠随机分为6组（每组10只）:正常组（N1,N2）给予普通饲料喂养;模型组（M1,M2）和治疗组（D1,D2）给予高脂饮食喂养;D1组在给予高脂饮食的基础上于0周时即给予降脂益肝冲剂,D2组于8周时给予降脂益肝冲剂,同时模型组和正常组分别给予等量的饮用水,于8周、12周末两个时点处死大鼠。测定血清丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）、总胆固醇（TC）、甘油三酯（TG）;测定空腹血糖（FBG）、空腹胰岛素（FINS）,计算胰岛素敏感指数（ISI）;HE染色观察肝组织病理变化;RT-PCR分别检测各组大鼠肝脏L-FABP的表达。结果:模型组大鼠血清ALT、AST、TC、TG升高,ISI降低,伴典型的脂肪性肝炎;与模型组相比,治疗组的各项指标比同期模型组都有所改善,两组间有统计学差异。模型组大鼠肝组织的L-FABP mRNA表达增高,与模型组相比治疗组L-FABP mRNA表达显著降低,说明降脂益肝冲剂能减少脂肪肝大鼠肝组织L-FABP mRNA的表达。结论:降脂益肝冲剂能通过调节L-FABP的表达来调节脂质代谢,改善胰岛素抵抗,降低肝脏的脂质沉积,有效地治疗大鼠非酒精性脂肪肝。关键词：非酒精性脂肪肝;肝脏型脂肪酸结合蛋白;降脂益肝冲剂
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发布时间：2024-09-23 - 摘要：Objective:Methods:Results:Conclusions:To study the effect of parenteral injection of milkvetch root saponin on myocardial contraction in experimental heart failure dogs.Methods:Acute heart failure model in anesthetic dogs was induced by using pentobarbital sodium,the central depressant.Parenteral injection of milkvetch root saponin was injected through vein.Then the heart function parameters,e.g.left ventricular systolic pressure（LVSP）,left ventricular end-diastolic pressure（LVEDP）,contractive pressure maximum upstroke velocity(+dp/dtmax),diastolic pressure maximum descent velocity(-dp/dtmax),and heart rate（HR） were determined.Results: Milkvetch root saponin could obviously increase heart failure dogs’ LVSP,+dp/dtmax,-dp/dtmax while it had no significant effect on LVEDP and HR.Conclusion: The injection of milkvetch root saponin can enhance myocardial contractility,improve the functions of myocardial contraction and relaxation,and it has obvious effect in treating acute heart failure.关键词：parenteral injection of milkvetch root saponin;Dog;acute heart failure;hemodynamics
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发布时间：2024-09-23 - 摘要：Objective:To explore the effects of Baoqingkang,a prescription for replenishing kidney,invigorating Qi and activating blood,on anti-stress ability,the learning and memory and the related enzyme activities in the aged mice.Methods:The various stress including fatigue resistance,normal hypoxia tolerance and heat resistance abilities assay was employed to evaluate the anti-stress ability of Baoqingkang;The baiting method was employed to measure the learning and memory of the aged mice;McEwen method was employed to measure cerebral MAOB activities of the middle age and the old age mice.TBA method,pyrogallol autoxidation method and DNBT method were employed to determine the liver MDA,SOD activities and GSH-Px activities in brain,liver,heart and whole blood of the aged mice,respectively.Results:Baoqingkang could prolonge the mouse swimming time,the survival time of hypobaric hypoxic mice,decrease the mortality in the heat resistance assay,increase the anti-stress ability of the mice.Baoqingkang could improve the learning and memory of the middle age and the aged mice,and decrease the liver MDA content for the aged mice,increase SOD and GSH-Px activities in brain,liver,heart and whole blood for the aged mice.Conclusion:Baoqingkang has anti-stress and anti-aging effect.关键词：Baoqingkang;MAO-B;MDA;SOD;GSH-Px
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发布时间：2024-09-23 - 摘要：目的:探讨抗纤软肝颗粒含药血清对肝星状细胞黏着斑激酶（FAK）mRNA、蛋白表达的影响。方法:运用细胞培养技术,以hsc-t6细胞系为研究对象,分为（1）空白包被+10%生理盐水血清组;（2）纤维连接蛋白（FN）+10%生理盐水血清组;（3）FN+5%抗纤软肝颗粒含药血清（kxr）组;（4）FN+10%kxr组;（5）FN+20%kxr组。按以上分组进行药物干预,干预后继续培养48 h。应用免疫细胞组织化学方法检测FAK蛋白的表达,采用RT-PCR法测定FAK mRNA的表达。结果:kxr组肝星状细胞的FAK mRNA、蛋白表达均显著下调,分别减少了72.78%和27.88%,78.82%和44.85%,85.51%和57.58%,与对照组及FN组相比,5%kxr组（P<0.05）,10%kxr组、20%kxr组（P<0.01）。结论:kxr能在转录及翻译水平抑制FAK mRNA、蛋白的表达,从而阻抑了整合素激活的FAK途径的信号转导。关键词：肝星状细胞;黏着斑激酶;纤维连接蛋白;抗纤软肝颗粒
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发布时间：2024-09-23 - 摘要：目的:比较多元线性回归、逐步多元回归（SMR）、BP网络（BPNN）、基于遗传算法的BPNN（GA-BPNN）、支持向量回归（SVR）和最小二乘SVR（LS-SVR）6种方法用于均匀设计（UD）优化的效果。方法:以某UD实验数据为研究载体,采用平均绝对百分比误差等为评价指标,对6种方法进行了精度、泛化能力及优化效率等的评价。结果:SMR方法简洁,需时少;BPNN稳健性较差,GA-BPNN性能较BPNN大为提高;SVR准确度高,泛化能力强,优于其他方法。结论:SMR、GA-BPNN、SVR和LS-SVR 4法均可较好地用于该载体的优化;对于变量较少、非线性不复杂而精度要求不苛刻时的UD优化,宜用SMR;而对于变量较多、非线性较为复杂而精度要求较高时,SVR较宜,其次GA-BPNN和LS-SVM。关键词：均匀设计;支持向量回归;最小二乘支持向量回归;BP神经网络;遗传算法;多元回归
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发布时间：2024-09-23 - 摘要：Objective:To research the Colour of tonge for HIV/AIDS patients by clinical epidemiology way.Method: Zonation,crowd method,and random parallel control were adopted for collecting and investigating the coated tongue feature of 1323 HIV/AIDS patients and 778 uninfected HIV control group.Result: The incidence rate of red tongue（42.6%） was highest,the second was carmoisine tongue（37.7%） in the patient group and the incidence rate of carmoisine tongue（54.2%） was highest,the second was red tongue（20.7%） in the control group.The incidence rate of replump tongue（19.8%） was highest for the tongue shape in the patient group.The changes in sublingual vessel was thick（13.8%） and thin（10.7%） in the patient group.The changes were statistically significant compared with the control group.Conclusion: Characteres of pathogenic factor HIV are turbid damp pyretic toxicity and stasis crientated.关键词：AIDS;colour of tonge;tongue shape;state of tong;sublingual vessel
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发布时间：2024-09-23 - 摘要：Objective:To observe the pulmonary protective effect and impacts of local chest radiotherapy in corpore levels of connective tissue growth factor（CTGF）and platelet-derived growth factor（PDGF） after Qingzaojiufei soup treatment in patients with advanced and mediate chest tumor.Methods: The patients with advanced-mediate chest tumors who were ready to local radiotherapy were randomly divided into treatment group and control group.Qinzaojiufei soup was taken for 6 months in the treatment group from the beginning of radiotherapy.The levels of plasma CTGF and PDGF were detected before and after radiotherapy.Clinical symptoms,high-resolution CT and pulmonary diffusing capacity were observed from 15th days after the beginning of radiotherapy.Results: There were significantly difference in the concentration of plasma CTGF and plasma PDGF（P<0.001）in control group compared with the treatment group after radiation therapy. There was statistically significant difference in CO diffusing capacity,acute radiation pneumonia and the incidence of pulmonary fibrosis in treament group compared with control group after the 5 months and 10 months radiotherapy（P<0.05）.Conclusion: Qinzaojiufei soup can inhibit over-liberation of plasma CTGF and PDGF induced by radiotherapy to reduce the deterioration of pulmonary diffusing capacity.关键词：radiotherapy;Qingzaojiufei soup;pulmonary injury;radiation protection;CTGF;PDGF
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发布时间：2024-09-23 - 摘要：目的:探讨祛疣洗剂防治尖锐湿疣的作用机制。方法:用免疫组化SP法检测祛疣洗剂治疗尖锐湿疣前后基底细胞和棘细胞的Ki-67表达以及表皮bax、bcl-2的表达,同时采用TUNEL法检测治疗前后表皮细胞的凋亡情况。结果:祛疣洗剂治疗后基底细胞和棘细胞增殖指数明显低于治疗前（P<0.01）,细胞凋亡指数治疗后高于治疗前（P<0.05）,而bax、bcl-2在表皮的表达在治疗前后无显著性差异。结论:祛疣洗剂可能是通过抑制表皮的基底细胞和棘细胞增殖以及促进表皮细胞的凋亡而发挥治疗作用的。关键词：祛疣洗剂;尖锐湿疣;细胞增殖;细胞凋亡
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- 50
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发布时间：2024-09-23 - 摘要：Objective:To identify disease in the standard based on a combination of indicators,research the relevance of cirrhosis of liver after hepatitis sub-standard to Chinese medicine and Child-pugh grade with pituitary gland thyroid gland axis hormone.Methods:The stochastic selection tallies brings into line with the standard the case of illness 120 examples to take the observation group and graded according to the Child-Pugh judgment classification,Testing T3,T4,rT3,FT3,FT4 and TSH.In Group 30 cases,both medical health.Statistical data and the subsequent analysis.Results: Spleen weakness wet abundant,gathering and brewing of dampness and heat in the middle burner,spleen kidney deficiency of yang group T3,FT3 liver kidney deficiency of yin and the vital energy of the liver depressed group obviously reduces,but rT3 liver kidney deficiency of yin and the vital energy of the liver depressed group obviously elevates,has the significance difference（P<0.01）;T3,FT3,the FT4 level in Child-pughA,B,the C three level of center assumes drops the tendency gradually,the rT3 level assumes by the level trend of escalation,during each group has the remarkable difference（P<0.01 or 0.05）.Conclusions: Cirrhosis of liver after hepatitis patient’s blood serums pituitary gland thyroid gland axis hormone level may appear the obvious change.The pituitary gland thyroid gland axis hormone level change may reflect hepatitis cirrhosis patient’s condition degree and the prognosis.关键词：Cirrhosis of liver after hepatitis;syndromes;pituitary gland thyroid gland axis hormone
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- 110
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发布时间：2024-09-23 - 摘要：Nonalcoholic fatty liver disease（NAFLD） is a sort of parenchymal lesion not caused by excessive consumption of alcohol.It includes simplex fatty liver,steatohepatitis,greasy hepatic fibrosis and cirrhosis.NAFLD encompasses a spectrum of common hepatic disorders in contemporary society with multiple pathologic consequences and is incidence is paralleling the increasing numbers of overweight and obese individuals worldwide.The pathogenesis of NAFLD is thought to be related mainly with insulin resistance syndrome and oxidative stress,and no effective drug can be obtained in the market for treatment of NAFLD.Large experimental studies on NAFLD are in progress recently.This review outlines the experimental studies about NAFLD and the advantage of traditional Chinese medicine in treatment of NAFLD.关键词：nonalcoholic fatty liver disease;pathogenesis;insulin resistance;Experimental Studies;animal model
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发布时间：2024-09-23 - 摘要：Objective:To investigate the pathogenetic role of poison in wilson’s disease.Methods: The understandings of poison from both Chinese traditional medicine and western medicine were collected,Damages to organism by poison,especially the pathogenetic role of poison in wilson’s disease,were discussed.Results: Poison is a common pathogenetic factor clinically and may cause damage to many organs.Wilson’s disease is a systemic disease which refers to multi-systems and organs,the accumulation of copper poison in the body is its pathophysiologic basis,and also the foundation of therapeutical method for disintoxicating.Conclusion: Poison plays a very important role in the pathogenetic factors for Wilson’s disease.The key pathogenesis of Wilson’s disease is the accumulation of copper poison in the body,and it cause the damage to the collaterals of liver,the collaterals of brain and the whole body’s collaterals.The direct disintoxication is an important method of treating Wilson’s disease.关键词：poison;hepatolenticular degeneration;pathogenesis
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