最新刊期
卷 20 ， 期 24 ， 2014
- 摘要：Objective: To compare difference of chemical components between traditional decotions and dispensing granules decoctions of Shaoyao Gancao decoctions. Method: Four types of 11 kinds of Shaoyao Gancao decoctions were prepared,including traditional decotions(TD),dispensing granules decoctions made by self(DGD-S),dispensing granule decoctions made by factory A and B (DGD-A,DGD-B),fingerprint was eastablished by HPLC,mobile phase consisted of acetonitrile(A)-0.05% phosphoric acid solution(B,pH of 2.42) with gradient elution(0-8 min,16%A;8-35 min,16%-50%A;35-36 min,50%-100%A;36-38 min,100%-16%A;38-40 min,16%A),detection wavelength was set at 250 nm.Taking average information of three batchese of TD for stabdard decoction information,difference informations of Shaoyao Gancao decoctions preparations were compared by five aspects,including appear or disappear of components,contents of index components,common peak area,principal component analysis and fingerprint similarity. Result: Compared with TD,composition of chemical components in DGDs did not change.The content of index components between three kinds of DGD and TD had great difference,especially difference of ammonium glycyrrhizinate were very notable.Total peak area comparison showed that sum of 23 total peak area between TD and DGD-A had no significant difference,but it had significant difference between TD and DGD-S,DGD-B,they were equivalent to 2.5 and 3.2 times of TD,respectively.In PCA plot,DGD-A was closer to TD which showed that the absolute amount of monomer composition was closer to TD,while the absolute amount of DGD-S,DGD-B were far more than TD.Similarity between DGD-A and standard decoctions was minimum,which showed that difference of components proportion between DGD-A and standard decoctions was maximum. Conclusion: Significant difference between TD and DGD only exists in proportion of ingredients,not related to components themselves,suggesting that more experiments should be done to validate whether its drug properties are changed and in clinical application,dosage must be reduced proportionally,so that curative effect is similar to TD.关键词：Shaoyao Gancao decoctions;traditional decotions;dispensing granules decoctions;principal component analysis;similarity analysis;ammonium glycyrrhizinate;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Mori Folium leaves in Yinsang Jiangtang granules. Method: Taking extracting amount of rutin as index, L9(34) orthogonal design was adopted to optimize alcohol extraction technology with extraction time,ethanol amount and concentration as factors.With relative yield of 1-deoxynojirimycin(DNJ) as index, L9(33) orthogonal design was used to optimize water extraction technology by taking solid-liquid ratio and extraction time as factor.The content of rutin was determined by HPLC,detection wavelength was 358 nm,mobile phase consisted of methanol(A)-0.5% phosphoric acid solution(B) for gradient elution(0-5 min,30%A;5-10 min,30%-35%A;10-15 min,35%-40%A;15-18 min,40%-50%A).The contents of DNJ were detected by precolumn derivation HPLC-UV method with FMOC-Cl as reagent. Result: Optimum alcohol extraction press was as following:extracted twice with 20 times the amount of 60% ethanol for 1.5 h;extracting amount of rutin was 1.22 mg·g-1.Optimum water extraction process was extracted twice for 1.0,0.5 h with 30,25 times the amount of water;relative yield of DNJ was 88.06%. Conclusion: These optimized extraction technologies are stable and feasible,it can balance extraction of flavonoids and alkaloids in Mori Folium.关键词：Yinsang Jiangtang granules;rutin;1-deoxynojirimycin;derivatization reaction
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Curcuma phaeocaulis,in order to provide experimental basis for dose-response change of components and mechanism research of Poxue Xingqi before and after being processed of this herb. Method: Taking extracting amounts of curcumin and volatile oil,dry extract yield as indexes,orthogonal design was adopted to optimize processing technology with boiling time,soaking time,amounts of vinegar and water as factors,then processing parameters were further optimized by artificial neural network model. Result: Optimum processing technology was as following:soaked 5 h with 20% vinegar and 6 times the amount of water,boiling time of 1.5 h;extracting amounts of curcumin and volatile oil,dry extract yield were 0.071 mg·g-1,0.96 mL and 26.68%,respectively. Conclusion: This process is stable and feasible with good repeatability,it can provide a reference for improving quality standard system of C. phaeocaulis.关键词：Sichuan authentic herb;Curcuma phaeocaulis;processing technology;artificial neural network model;curcumin;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of steamed Polygonati Rhizoma contained in 2005 edition of ‘Guizhou Province Chinese Herbal Pieces Processing Specification’,then to compare with single steaming processing method and multiple steam drying processing method. Method: Taking composite score of water-soluble extract,ethanol-soluble extract,total polysaccharides and total saponins as index,steaming process of Polygonati Rhizoma contained in 2005 edition of ‘Guizhou Province Chinese Herbal Pieces Processing Specification’ was optimized by factorial experiment,and compared with other processing methods.UV was adopted to determine contents of total polysaccharides and total saponins with detection wavelength of 582,543 nm,respectively. Result: Optimum processing technology was as following:steamed 8 h,moistened 12 h;composite score of this optimized process was significant higher than single steaming 6 h method and four steaming four processing method.The mass fractions of water-soluble extract were 70.21%,69.43% and 74.42%,ethanol-soluble extract were 70.09%,67.12% and 73.37%,total polysaccharides were 12.55%,11.37% and 10.57%,total saponins were 12.26%,9.73% and 8.41%,respectively. Conclusion: This optimized process about 2005 edition of “Guizhou Province Chinese Herbal Pieces Processing Specification” is superior to the other two ways.关键词：steamed Polygonati Rhizoma;factorial experiment;processing method;total polysaccharides;total saponins
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation of icariin magnetic nano-liposomes and investigate their in vitro release. Method: Ultrasonic film dispersion method was used to prepare icariin magnetic nano-liposomes,with encapsulation efficiency of icariin as index,based on single factor tests,orthogonal design was adopted to optimize preparation procedure by taking hydration medium and amounts of poloxamer 188(F-68),PEG-2000,oleic acid-modified magnetic powder.HPLC was employed to investigate in vitro release of icariin magnetic nano-liposomes in phosphate buffer solution(PBS) containing 30% methanol,mobile phase consisted of acetonitrile-water(30:70) and detection wavelength was set at 270 nm. Result: Optimum formulation was as follows:icariin-magnetic powder(2:1),F-68 of 200 mg,PEG-2000 of 60 mg,lecithin of 500 mg,cholesterol of 150 mg,PBS(pH 7.4) as hydration medium.Entrapment efficiency of icariin magnetic nano-liposomes was (96.57±0.42)%;cumulative release rate of icariin solution was near 99.68% in 16 h,but it was over 96.51% in 72 h for icariin magnetic nano-liposomes. Conclusion: Icariin magnetic nano-liposomes have high encapsulation efficiency and a good sustained release by ultrasonic film dispersion method.关键词：icariin;magnetic nano-liposomes;encapsulation efficiency;in vitro release;ultrasonic film dispersion method
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of children anti-sweating granules and provide a reference for clinical application of this preparation. Method: UV was adopted to determine the content of total saponins with detection wavelength of 541 nm.Children anti-sweating granules was prepared by extrusion granulation method,with total saponin content and dissolving time as comprehensive evaluation index,orthogonal test was adopted to optimize preparation process by taking water extraction time,alcohol precipitation concentration,ratio of dextrin-extract as factors,quality of this preparation was evaluated. Result: Optimal preparation process was as following:water extraction for 5 h,alcohol precipitation volume fraction of 60%,extract-dextrin(1:1.5);three batches of these granules had uniform particle size with brown,slightly sweet taste and consistent color,without moisture absorption,caking and deliquescence phenomenon.Dissolving time of these granules was 194 s,moisture content was 4.75%,the content of total saponins was 56.13 mg·g-1. Conclusion: This preparation process is stable,repeatable and feasible for children anti-sweating granules.关键词：children anti-sweating granules;sweating syndrome;total saponins;Z-comprehensive scoring method;dissolving time
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发布时间：2024-01-04 - 摘要：Objective: To optimize degreasing technology of extracts from Selaginella moelledorfii with petroleum ether,which provides a reference to development and utilization of the plant resources. Method: Taking extraction amount of amentoflavone and yield of extract as evaluation indexes,effects of the amount of petroleum ether,extraction time and frequency on degreasing technology were investigated by orthogonal design.The content of amentoflavone was determined by HPLC,mobile phase consisted of acetonitrile(A)-water(B) for gradient elution(0-5 min,25%A;5-12 min,25%-35%A;12-17 min,35%-45%A;17-25 min,45%-50%A;25-40 min,50%-55%A;40-45 min,55%-70%A;45-50 min,70%-100%A;50-55 min,100%A;55-60 min,100%-25%A) and detection wavelength at 330 nm. Result: Optimum technology conditions were as follows:degreased twice with 6 times the amount of petroleum ether for 1 h per time;extraction amount of amentoflavone was 72.77 mg,yield of extract was 11.04%. Conclusion: This degreasing technology is rational and stable,which is available for purification of extracts from S. moelledorfii.关键词：Selaginella moelledorfii;amentoflavone;degreasing process;extract yield
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发布时间：2024-01-04 - 摘要：Objective: Stability of preparation process of total flavonoids and total saponins from Ziziphi Spinosae Semen was investigated,in order to provide a guarantee for sample preparation of follow-pharmacodynamic evaluation. Method: Ten batches of total flavonoids and total saponins from Ziziphi Spinosae Semen were prepared with the same preparation process.Spinosin,betulinic acid,jujuboside A and jujuboside B were taken as index components,ELSD/HPLC was employed to analyze feature profiles of these two parts samples with mobile phase of acetonitrile(A)-water(B) for gradient elution(0-10 min,12%-18%A;11-20 min,19%-23%A;21-30 min,23%-34%A;31-40 min,35%-45%A;41-50 min,46%-100%A;51-60 min,100%-11%A;61-70 min,12%A) and drift tube temperature of 40 ℃. Result: Chemical compositions distribution of total flavonoids part focused on the fomer 25 min,but that of total saponins concentrated in after 25 min,purities of total flavonoids and total saponins were more than 50%,peak at 54.46 min was identified as betulinic acid. Conclusion: This preparation technology of total flavonoids and total saponins from Ziziphi Spinosae Semen is stable,which can be used as samples of pharmacodynamic experiment.关键词：total flavonoids;total saponins;spinosin;betulinic acid;Ziziphi Spinosae Semen;stability
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发布时间：2024-01-04 - 摘要：Objective: To optimize enrichment technology of total lignans from Arctii Fructus residue after subcritical extraction. Method: Taking yield of Arctii Fructus oil and the content of total lignans in residue as indexes,subcritical extraction solvent of Arctii Fructus was investigated by single factor test.Total lignans in residue was enriched by alcohol extraction and water precipitation method,process parameters was optimized by single factor tests.HPLC was employed to determine contents of arctiin and arctigenin with mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-5 min,30%-35%A;5-13 min,35%-45%A;13-15 min,45%-48%A;15-20 min,48%-53%A;20-29 min,53%-80%A;29-40 min,80%-100%A) and detection wavelength at 280 nm. Result: Butane was selected as extraction solvent for degreasing.Optimum enrichment process was as follows:reflux extracted thrice with 8 times the amount of 70% ethanol for 1 h each time,filtrate pressure recoveried to 0.5 g·mL-1,precipitation with 12 time the amount of water according to quantity of dregs,reflux boiling time of 60 minutes;purity of total lignans was more than 50%,transfer rate reached more than 80%. Conclusion: Alcohol extraction and water precipitation method is suitable for enrichment and purification of total lignans in Arctii Fructus,this optimized process is stable and feasible.关键词：Arctii Fructus;subcritical extraction technology;arctiin;arctigenin;total lignans
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发布时间：2024-01-04 - 摘要：Objective: To prepare baicalin thermosensitive gels for external application through different mathematical models and evaluation indexes. Method: Taking poloxamer 407(P407) and poloxamer 188(P188) as gel matrices,gelation temperature was applied as a target to compare formulations of baicalin thermosensitive gels,which were optimized by all sample multiple linear regression model and central composite design-response surface methodology(CCD-RSM),while combination of gelation time and length were adopted to optimize formulation. Result: Counting on high reliability of CCD-RSM.Gels formulation was finally confirmed as 1% baicalin,17% P407 and 1.5% P188;gelation temperature,time and length were (30.3±0.1) ℃,(18.9±1.9) s,( 0.7±0.6) cm,respectively. Conclusion: Baicalin thermosensitive gels for external application is consistent with external requests for rapid enrichment at the local and elevated compliance,index evaluation should be based on results of human determination,references are offered to filter prescriptions of external thermosensitive gels scientifically and reasonably.关键词：baicalin;external thermosensitive gels;all sample multiple linear model;gelation temperature;gelation length
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of the contents of polygalaxanthone Ⅲ and 3,6'-disinapoyl sucrose. Method: Separation was performed on ZORBAX Eclipse plus C18 column(4.6 mm×100 mm, 3.5 μm) with the following settings:mobile phase cetonitrile-0.1% formic acid (28:72);flow rate 0.3 mL·min-1;the injection volume 10 μL;column temperature 20 ℃. MS condition, electrospray ionization (ESI) and multiple reaction monitoring (MRM) were adopted. The most abundant precursor/production pairs were m/z 567.2-345.1 for polygalaxanthone Ⅲ, and m/z 753.3-205.1 for 3,6'-disinapoyl sucrose. Result: The two components showed good linearity and the analyzing process was just within 6 min. The intra-day precision and inter-day precision for polygalaxanthone Ⅲ were 2.2% and 2.5% respectively;the recoveries were between 96.1% and 101.7% with RSD 2.4%.While the intra-day precision and inter-day precision for 3,6'-disinapoyl sucrose were 2.3% and 2.4%;the average recoveries were in the range of 95.7%-101.4% with RSD 2.0%. Conclusion: The method is rapid, sensitive, selective and suitable for the quantitative analysis of polygalaxanthone Ⅲ and 3,6'-disinapoyl sucrose in polygala tenuifolia.关键词：polygalaxanthone Ⅲ;3,6'-disinapoyl sucrose;Polygala tenuifolia;HPLC-MS/MS
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to purify and determine the content of dubioside F in the tuber of Thladiantha dubia from different habitats by HPLC Method: Dubioside F was purified by column chromatograph with silica gel(CHCl3-MeOH-H2O 14:7:1)and identified through physicochemical properties and spectral analysis. To determine the content of dubioside F in the tuber of T. dubia from different habitats by HPLC:A Discovery-C18 (4.6 mm×250 mm, 5 μm) column was used with the mobile phase of acetonitrile-water (24:76). The DAD detector was set at 200 nm. Result: Dubioside F showed good linearity (r=1) within test ranges(0.98-24.5 μg). The average recovery was 96.96%, with RSD of 1.1%. Conclusion: This method was well validated in good precision, repeatability and sensitivity. The most abundant dubioside F in the tuber of T. dubia in different cultivated habitats was from Helong of Jilin rovince;while the most abundant dubioside F in the tuber of T. dubia in different natural habitats is from Kuancheng of Hebei province. This method was successfully applied to quantify dubioside F in the tuber of T. dubia.关键词：tuber of Thladiantha dubia;dubioside F;HPLC;determination
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发布时间：2024-01-04 - 摘要：Objective: The study aimed to establish the quality standards of Qiakekute granular. Method: Phellodendri Amurensis Cortex and Vaccariae Semen were identified by TLC. The contents of vaccarin, berberine hydro-chlorid, palmatine hydrochloride and ammonium glycyrrhizinate were determined by HPLC. Result: The TLC spots were clear and specific in good repeatability without inference from negative sample. The linear range of vaccarin was 7.204 0-108.059 3 mg·L-1(r=0.999 5) with an average recovery of 98.95%(RSD 2.2%). The linear range of berberine hydrochloride was 20.432 7-408.654 4 mg·L-1(r=0.999 6) with an average recovery of 101.83% (RSD 1.6%). The linear range of palmatine hydrochloride was 12.109 1-242.182 1 mg·L-1(r=0.999 5) with an average recovery of 98.24% (RSD 1.1%). The linear range of ammonium glycyrrhizinate was 0.061 08-0.407 2 g·L-1(r=0.999 5) with an average recovery of 100.10% (RSD 0.8%). The RSDs of precision, stability and reproducibility tests were all lower than 2%. Conclusion: The standard established in this study can be used for the quality control of Qiakekute granular.关键词：Qiakekute granules;TLC;HPLC;quality standards
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this research was to evaluate the performance of Daidai flavones self-microemulsifying pellets, and to establish a method for the determination of Daidai flavones in this preparation. Method: The performance was evaluated by appearance, size distribution, droplet size, self-emulsification efficiency, zeta potential,stability and influence factors after forming micro emulsion. The neohesperidin was used as index ingredient, and the content of total flavones was measured by UV-sectrophotometry at 284 nm. Result: The size of Daidai flavones SME pellets in this research mainly distributed at 16-30 sieves and the yield reached up to 92.43%, the end product was uniformly spherical yellowish-brown pellets. The droplet sizes after forming micro emulsion were nearly the same as those in different dispersion mediums, at different rotate speeds and at different temperatures. The average diameter of Daidai flavones SME pellets were (78.8±12) nm. The UV method for determination of total flavones content was established. The linear ranges of neohesperidin was 4.44-26.64 mg·L-1 (r=0.999 9) with the average recoveries of 99.9% (RSD 1.0%). Conclusion: The Daidai flavones self-microemulsifying pellets have good self-microemulsifying performance. The UV method is simple, accurate and reliable, can be well used for the determination of total flavones in Daidai flavones self-microemulsifying pellets.关键词：daidai flavones;self-microemulsifying drug delivery system;pellet;performance evaluation;total flavones
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to develop an HPLC method for the simultaneous determination of contents of scutellarin, protopine, apigenin and tetrahydropalmatine in Dengyan Granules. Method: The analysis was performed on a Zorbax Extend-C18 column (4.6 mm×250 mm,5 μm) in gradient elution (0-25 min,15%A;25-70 min,15%-65%A;70-75 min,65%-25%A) by using the mobile phase of acetonitrile(A)-0.3% ammonium acetate solution(B).The flow rate was 0.8 mL·min-1 and the column temperature was maintained at 30 ℃,the detection wavelengths were set at 280 nm (for scutellarin,protopine and tetrahydropalmatine),337 nm (for apigenin), respectively. Result: The calibration curves were linear within the range of 0.90-8.99 μg for scutellarin, 0.73-7.35 μg for protopine, 0.05-0.54 μg for apigenin and 0.14-1.37 μg for tetrahydropalmatine,respectively. The recoveries of scutellarin, protopine, apigenin and tetrahydropalmatine were 99.48% (RSD 0.8%), 98.17% (RSD 1.2%), 97.41% (RSD 1.2%) and 98.33% (RSD 0.5%), respectively. Conclusion: The method can be used for simultaneous determination of contents of scutellarin, protopine, apigenin and tetrahydropalmatine in Dengyan Granules.关键词：scutellarin;protopine;apigenin;tetrahydropalmatine;HPLC
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发布时间：2024-01-04 - 摘要：Objective: An HPLC method was established to determine the content of pueratin in three types of injection. Method: Separation was achieved by a Merk, Purospher STAR RP-C18 column used at 29 ℃, and mobile phase was methanol and 1% acetic acid, water with the flow rate of 1.0 mL·min-1, detection wavelength of 250 nm. Result: The puerarin was separated with good linearity in the sample size range of 0.192-1.152 μg. The average recovery was 99.81%, with RSD value of 0.42%. Conclusion: The puerarin in three types of puerarin injection could be well separated under the selected conditions, showing good linear relationship. The method was simple and quick, feasible and could provide scientific basis for the quality control of puerarin injection.关键词：HPLC;Puerarin injection;puerarin and glucose injection;puerarin and sodium chloride injection;puerarin
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish an HPLC method for simultaneous determination of the content of four flavonoid glycosides in Shanlamei Qinggan Cha. Method: Using an Elite hypersil ODS2 column(4.6 mm×250 mm,5 μm), the mobile phase was acetonitrile-0.1% acetic acid, with flow rate of 1.0 mL·min-1. The detective wavelength was at 344 nm and the column temperature was 35 ℃. Result: The standard curve presented a linear range from 7.012-70.12 mg·L-1 for rutin(r=0.999 6),the average recovery was 100.68% with RSD of 3.27%.The calibration curve was linear in the range of 5.126-51.26 mg·L-1 for kaempferol-3-O-β-D-galactose -(6-1)- α-L-rhamnoside (r=0.999 9), the average recovery was 98.48% with RSD of 1.7%. The calibration curve was linear in the range of 2.631-26.31 mg·L-1 for kaempferol-3-O-rutinoside (r=0.999 8), the average recovery was 101.82% with RSD of 1.9%. The standard curve presented a linear range from 2.634-26.34 mg·L-1 for astragalin (r=0.999 9), the average recovery was 97.02% with RSD of 1.7%. Conclusion: The method was fast, accurate and simple in good reproducibility, which could be used to control the quality of Shanlamei Qinggan Cha.关键词：rutin;kaempferol-3-O-rutinoside;astragalin;kaempferol-3-O-β-D-galactose-(6-1)-α-L-rhamnoside;Shanlamei Qinggan Cha
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish a method for the content determination of chlorogenic acid, liquiritin, hesperidin, arctiin and phillyrin in Yinqiao Powder. Method: UPLC system was performed on ACQUITY UPLC BEH Shield RP18 column(2.1 mm×50 mm,1.7 μm)at 40 ℃. The mobile phase was consisted of methanol-0.15% formic acid solution in gradient elution mode. The flow rate was set at 0.25 mL·min-1 and the detection wavelength was set at 279 nm. Result: Chlorogenic acid, phillyrin, arctiin, hesperidin and liquiritin had good linear relationship in the ranges of 0.018 76-0.043 77, 0.002 370-0.011 85, 0.011 21-0.056 06, 0.001 34-0.006 71 and 0.001 71-0.012 38 μg, respectively. The corre-lation coefficients were all more than 0.999 1.The average recoveries were 97.12%(RSD 0.9%), 101.40%(RSD 2.1%), 100.61%(RSD 1.6%), 100.79%(RSD 0.7%)and 101.04%(RSD 1.7%), respectively. Conclusion: The method is simple, rapid, specific, and can be used for quality control of Yinqiao powder.关键词：UPLC;Yinqiao powder;chlorogenic acid;phillyrin;arctiin;hesperidin;liquiritin
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发布时间：2024-01-04 - 摘要：Objective: To compare the differences of HPLC profile and the main components among 3 different solvents so as to provide the basis for improving the quality standard of Chai-huang granules. Method: HPLC was employed to identify the main chromatographic peaks, which also was taken to compare the relative peak amounts, total amounts as well as the relative ratio among water, methanol and 70% methanol solvent extracts. Result: Four peaks (baicalin, wogonoside, baicalein, wogonin) were identified. HPLC profiles of 3 extract solvents were almost same, however there were exhibited the significant varieties of the peak amounts, total amounts and the relative ratio of 4 major components. The contents of 3 extract solvents had substantial differences, and water as extracting solvent was highest. Conclusion: This study could offer the useful reference for determination method of Chai-huang granules.关键词：Chai-huang granules;baicalin;wogonoside;baicalein;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of chlorogenic acid, berberine, baicalin, oleanolic acid and ursolic acid in Xiongju Shangqing tablets. Method: The separation was performed on a DIKMA Platisil ODS column (4.6 mm×250 mm, 5 μm) with gradient elution using methanol and 0.1% H3PO4 aqueous solution as mobile phase. The flow rate was 1 mL·min-1 and the detection wavelengths were 327 nm for chlorogenic acid, berberine and baicalin; 210 nm for oleanolic acid and ursolic acid, respectively. Result: Linear ranges of chlorogenic acid, berberine, baicalin, oleanolic acid and ursolic acid were 0.03-0.6,0.011 4-0.228,0.025-0.5,0.1-2,0.03-0.6 μg, respectively. Their average recoveries were 99.57%(RSD 1.3%),99.19%(RSD 0.8%),99.14%(RSD 0.6%),99.38%(RSD 1.2%),98.92%(RSD 0.7%),respectively. Conclusion: The established HPLC method can be used to determine the contents of chlorogenic acid, berberine, baicalin, oleanolic acid and ursolic acid in Xiongju Shangqing tablets. The method can also be used as the standard for the quality control of Xiongju Shangqing tablets.关键词：HPLC;Xiongju Shangqing tablets;chlorogenic acid;berberine;baicalin;oleanolic acid;ursolic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish the determination method of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol,and to compare the content of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol in different batches of dalbergiae odoriferae oil. Method: Gas chromatography, DB-Wax fused silica capillary column (250 μm × 30 m, 0.25 μm), with FID detector, internal standard was naphthalene. Result:The content of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol in dalbergiae odoriferae oil were 19% to 25%, 16% to 20%, 25% to 30%. Oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol were good linear separately within the concentration range of 0.030 4-0.114, 0.030 4-0.114, 0.020 8-0.078 g·L-1. The average recoveries were 99.27%, 99.41%, 98.68%. Conclusion: The GC method was used to determine the content of oxidation nerolidol Ⅰ,oxidation nerolidol Ⅱ,trans nerolidol in dalbergiae odoriferae oil is simple and feasible.There is no apparent difference in the content of seven batches of the dalbergiae odoriferae oil.
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the content determination of perillaldehyde in Suhuang Zhike Capsule. Method: HPLC was performed on SHIMADZU Shim-pack VP-ODS C18 column (4.6 mm × 250 mm, 5 μm), and the mobile phase was consisted of methanol-water (68:32). The detection wavelength was set at 230 nm, the flow rate of 1.0 mL·min-1 and the column temperature was 30 ℃. Result: Perillaldehyde showed a good linear relationship from 5.672 ng to 453.76 ng, and the regression equation was Y=5.695X+1.28 (r=0.999 5). The average recovery was 99.57 % with RSD of 1.68 %. Conclusion: The HPLC method is simply and accurate with a good specificity. It can be used to determine the content of perillaldehyde in the Suhuang Zhike Capsule.关键词：Suhuang Zhike capsule;HPLC;Perillaldehyde
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发布时间：2024-01-04 - 摘要：Objective: To prepare Gushangning ointments and establish its quality control method. Method: Gushangning ointments was prepared by emulsification method.Qualitative analysis of Gushangning ointments was detected by TLC and precipitation reaction.Contents of notoginsenoside R1 and ginsenoside Rg1,Rb1 in Gushangning ointments by HPLC,mobile phase consisted of acetonitrile(A)-water(B) for gradient elution(0-12 min,19%A;12-60 min,19%-36%A;60-70 min,36%-19%A;70-73 min,19%A),dection wavelength was set at 203 nm. Result: Gushangning ointments had stable traits,linear ranges of notoginsenoside R1 and ginsenoside Rg1,Rb1 were 0.15-3.75,0.45-11.25,0.42-10.5 μg,average recoveries of these three components were 98.81%(RSD 3.65%),101.18%(RSD 2.26%),98.90%(RSD 2.92%),respectively. Conclusion: This established qualitative analysis is reliable and accurate,HPLC is reliable,simple and precise,which are suitable for quality control of Gushangning ointments.关键词：Gushangning ointments;emulsification method;quality control;notoginsenoside R1;Ginsenoside Rg1;ginsenoside Rb1
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发布时间：2024-01-04 - 摘要：Objective: To compare contents of norepinephrine and dopamine in fresh and dry Portulacae Herba. Method: HPLC-UV method was adopted for determination of norepinephrine and dopamine in fresh and dry Portulacae Herba.This analysis was performed on a Alltima C18 column(4.6 mm×250 mm,5 μm),column temperature was set at 25 ℃,flow rate was 0.8 mL·min-1,detection wavelength was kept at 280 nm, mobile phase consisted of methanol-water(70:30,containing 30 mmol·L-1 sodium dodecyl sulfate and 0.02 mol·L-1 phosphate buffer with pH of 3.2). Result: Separation of norepinephrine and dopamine was well in HPLC,these two alkaloids showed a good linear relationship in 52.5-157.5 mg·L-1 and 64.7-194.1 mg·L-1,respectively.Contents of norepinephrine and dopamine in fresh Portulacae Herba were 0.070% and 0.50%;while contents of them in dry Portulacae Herba were 0.015% and 0.20%,respectively. Conclusion: Contents of norepinephrine and dopaminein in fresh herbs are significantly higher than these in dry herbs.This paper provides experimental basis for reasonable application of Portulacae Herba.关键词：Portulacae Herba;fresh and dry herbs;norepinephrine;dopamine
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发布时间：2024-01-04 - 摘要：Objective: To establish a TLC Identification of Trollius chinensis and determination of total flavonoids in the herb. Method: Extraction solvent and deployment system in TLC identification method of T. chinensis was screened by single factor tests.A colorimetry was adopted to determine the content of total flavanoids in T. chinensis by taking orientoside as index component,10% aluminum nitrate solution as color developing reagent and detection wavelength at 510 nm,then content difference of total flavanoids in T. chinensis from different origins was compared. Result: Developing agent was chloroform-methanol-butanone-acetic acid(4:2:3:3), this established TLC had a good separation effect of each component.Orientoside had a good linear relationship in the range of 8.096-56.672 mg·L-1,average recovery was 98.8%;the mass fractions of total flavanoids in No. 1,2,3,4,5,6 sample were 9.79%,8.71%,8.32%,6.47%,6.42% and 6.71%,respectively. Conclusion: These methods are simple,accurate and applicable for quality control of T. chinensis.关键词：Trollii chinensis;orientin;vitexin;total flavonoids;TLC;colorimetry
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发布时间：2024-01-04 - 摘要：Objective: To improve HPLC for quality control of Gardeniae Fructus. Method: HPLC system consisted of a Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5 μm) and mobile phase of acetonitrile(A)-0.2% phosphoric acid(B) with gradient elution(0-15 min,6%-16%A;15-22 min,16%-21%A;22-34 min,21%-24%A;34-50 min,24%-42%A),monitoring wavelength was set at 238,440 nm for geniposide and crocin-1,respectively. Result: The content of geniposide in Gardeniae Fructus collected from different areas ranged from 23.025-53.693 mg·g-1,this result conformed to requirements (no less than 1.8%) in 2010 edition of “Chinese Pharmacopoeia”;but the content of crocin-1 in two samples(1.963,3.434 mg·g-1) were lower than others,color of these two samples were gray-brown and brown-yellow,them should be substandard medicine. Conclusion: The content of geniposide as only standard for quality control of Gardeniae Fructus is not rational,the content of crocin-1 should be also taken into consideration.This paper may be useful for editing the new edition of ‘Chinese Pharmacopoeia’.关键词：Gardeniae Fructus;geniposide;crocin-1;appearance color
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发布时间：2024-01-04 - 摘要：Objective: To investigate the constituents of the whole herb of Paederia scandens in Taibai mountain. Method: The compounds were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and recrystallization. Their structures were identified by physicochemical properties and spectra analysis. Result: Thirteen compounds were obtained and identified as β-sitosterol (1), stigmast-5-ene-3,7-diol (2), ursolic acid (3), 3-oxours-12-en-28-oic acid (4), oleanolic acid (5), dotriacotane (6), daucosterol (7), taraxerol (8), salicylic acid (9), 3-O-acetyloleanolic acid (10), succinic acid (11), azelaic acid (12), paederoside (13). Conclusion: Compounds 2, 4, 6, 8-10 and 12 were obtained from the Paederia scandens for the first time.关键词：rubiaceae;Paederia scandens;chemical constituent;triterpenoids;steroids
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from Citrullus colocynthis. Method: The compounds were separated and purified from Citrullus colocynthis by using a various chromatographic techniques including colum chromatography over silica gel, ODS and preparative HPLC. Their structures were elucidated by spectroscopic techniques including 1H-NMR,13C-NMR, 2D-NMR, ESI-MS and HRESI-MS. Result: Nine compounds were isolated from this plant and their structures were identified as nicotinamide(1), uracil(2), isocucurbitacin B(3), cucurbitacin E(4), dihydro-cucurbitacin E(5), cucurbitacin E-2-O-β-D-glucopyranoside(6), khekadaengoside E(7), cucurbitacinK-2-O-β-D-glucopyranoside(8), deoxocucurbitoside B(9). Conclusion: Compounds 1-3 were isolated from this plant for the first time.关键词：Citrullus colocynthis;chemical constituents;triterpene;cucurbitane glycosides
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发布时间：2024-01-04 - 摘要：Objective: To study the major chemical constituents from the underground part of Isodon flavida. Method: Compounds were isolated and purified from ethyl acetate fraction of 70% acetone extract of the underground part of I. flavida using silica gel, sephadex LH-20, and MCI column chromatographic techniques. Their structures were determined by comparison of physicochemical properties and analysis of spectroscopic data. Result: Eleven compounds were obtained and identified as squalene (1), stigmast-4-en-3-one (2), stigmast-4-ene-3,6-dione (3), 8(17),13-ent-labdadien-15→16-lactone-19-oic acid (4), 1-monoolein (5), 2α-hydroxyl ursolic acid (6), maslinic acid (7), 2α,3α,23-trihydroxyursa-12,20(30)-dien-28-oic acid (8), stigmasterol (9), 7α-hydroxy-sitosterol (10), and β-sitosterol (11). Conclusion: All of these compounds except for 7 were isolated from the plant for the first time. This study will be helpful for the better understand of the chemical properties of the underground part of Isodon plants.关键词：Isodon flavida;chemical constituents;structural identification
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to investigate the interaction of (E)-2-[2-(4-methoxybenzylidene)hydrazinyl]-4-phenylthiazole(MHP) and human Albumin(HSA),in order to provide experimental basis for revealing the mechanism of interaction between MHP and HSA. Method: The impactof MHP on HSA conformation was investigated by using synchronous fluorescence spectroscopy, based on Stern-Volmer equation and the theory of non-radiative energy transferring. Result: The experiment result showed that MHP can lead to the enhancement of HSA fluorescence, indicating energy transferring between them. And the mechanism of energy transferring is that MHP and HSA formed complexes. Fluorescence enhancement (sensitization effect) mainly resulted from the-energy transferring of dipole-dipole effect between donor and receptor. Conclusion: The results of the energy transferring were the fluorescence quenching of endogenous chromophoric group, and the fluorescence sensitization of exogenous chromophoric group, and the sensitization constant was calculated as -2.620 8×104.Synchronous fluorescence spectra showed that the interaction between HSA and HA could cause the HSA conformation change, implying the binding sites closer to tryptophan.关键词：(E)-2-[2-(4-methoxybenzylidene)hydrazinyl]-4-phenylthiazole(MHP);albumin human;fluorescence spectroscopy;synchronous fluorescence spectrum
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发布时间：2024-01-04 - 摘要：Objective: To study the interaction of salbutamol sulfate (SAS) with human serum albumin (HSA) and human immunoglobulin G (HIgG). Method: Qualitative and quantitative structure changes of HSA and HIgG were performed by molecular modeling, UV-visible absorbance and circular dichroism spectroscopy methods. Result: The experiment results obtained from molecular modeling indicated that there were hydrogen bonds, Van der Waals force and electrostatic interactions between SAS and HSA;meanwhile, hydrogen bonds, π-cation interaction and Van der Waals force played the major role in the interaction of SAS and HIgG. Experimental results observed from UV-visible absorbance spectroscopy showed that the secondary structures of HSA and HIgG were altered in the presence of salbutamol sulfate, and their quenching mechanisms were both suggested as static quenching. Moreover, the α-helices of HSA in the presence of SAS were decreased from 47.92% to 46.53 %, and the secondary structure of HIgG was also changed because of the addition of salbutamol sulfate, which were obtained from the circular dichroism spectroscopy. Conclusion: Molecular modeling, UV-visible absorbance and circular dichroism spectroscopy are good methods for investigating the interaction between SAS, HSA and HIgG. They have the advantages of high speed and high sensitivity.关键词：salbutamol sulfate;human serum albumin;human immunoglobulin G;molecular modeling;spectroscopy
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发布时间：2024-01-04 - 摘要：Objective: To analyze components and metabolites in serum of rats after given of Mume Fructus by gavage. Method: Eight female rats were divided into Mume Fructus group and blank control group, former group were given Mume Fructus crude solution(0.633 6 g·mL-1 )2 mL·kg-1 by gavage, the latter with pure water. Four days later, solid phase extraction method was used to extract the drug-containing serum and blank serum, using Mume Fructus extracted by acetonitrile as medicinal materials control. Chromatography using Angient Zorbax Eclipse Plus C8(3.0 mm×100 mm, 1.8 μm) for chromatographic column, using 0.1% formic acid solution-acetonitrile gradient elution, flow rate of 0.5 mL·min-1. Mass spectrometry using ESI source, within the scope of 50-1 200 m/z positive scanning mode. Mass spectrum data were preprocessed and principal component analyzed by Mzmine 2.9 software, using the results of principal component analysis and the database of prototype component to identify blood transitional component and metabolites. Result: In serum there were three components from the Mume Fructus original medicinal herbs(such as ursolic acid or oleanolic acid, medicagenic acid), and there were 10 kinds of the metabolites. Conclusion: This method can be used for analysis of components of medicinal materials into the blood and its metabolites more objective and comprehensive, and it can be used to provide reference and basis for pharmacodynamics research of the Mume Fructus material.关键词：Mume Fructus;high performance liquid chromatography-mass spectrometry;principal component analysis;serum pharmacochemistry
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发布时间：2024-01-04 - 摘要：Objective: To study the intestine absorption of punicalagins. Method: The isolated everted intestine model was used to study the absorption of different drug concentration(153.34,301.70 mg·L-1)in different regions of rat intestine(duodenum,jejunum,ileum and colon), and different time(0,30,60,90,120 min).The sample was determined with HPLC on an YMC-Triart C18 column(4.6 mm×250 mm,5 μm)with methanol-0.1%phosphoric acid. Result: The intestine absorption rate constant increased along with the increase in concentration of punicalagins.Absorption of index components for punicalagins in different intestinal segments had no significant difference,at low concentration, the absorption rate constant is ileum>colon>jejunum>duodenum, at high concentration, jejunum>colon>ileum>duodenum;every intestine segments cunmlative absorption increased along with concentration of punicalagins,at high concentration,every intestine segments cunmlative absorption of punicalagins is (0.60±0.10),(0.64±0.59),(0.71±0.13),(0.73±0.11) μg·cm-2. Conclusion: Absorption of punicalagins didn't be a special ‘absorption window’, punicalagins intestinal absorption followed the Zero-order kinetic equation.关键词：punicalagins;isolated evened gut model;intestine absorption;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an effective method for determining the strychnine of Tongmai pill concentrate (TPC) in rat plasma by UPLC. Method: The rat plasma samples were processed by liquid-liquid extraction and separated on a BEH C18 column. The mobile phase consisted of 23% acetonitrile and 77% water containg 0.02 mol·L-1 potassium dihydrogen phosphate and 0.01 mol·L-1 sodium heptanes sulfonate (adjusting pH 2.5 with 10% phosphonic acid.The flow rate was 0.18 mL·min-1. The detection wavelength was set at 260 nm and the column temperature was 30 ℃. Result: The determination of strychnine was not interferred by the endogenous in the rat plasma. The calibration curve of strychnine was in good linearity within the range of 0.043 2-10.8 mg·L-1. The mean extraction recoveries were more than 85% and intra-and inter-day precisions were less than 10%. The stability results meet the requirements for the biopharmaceutical analysis. Conclusion: This sensitivite and precise analytical method is applicable to the pharmacokinetic study of strychnine of TPC in rat plasmas.关键词：strychnine;biopharmaceutical analysis;UPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of ginsenosides Rb1 on memory impairment of type 2 diabetes. Method: Sixteen 10-week C57BL mice were as normal group (N group) and 64 Kkay mice 10-week with high blood sugar (≥13.9 mmol·L-1) as model group (M group), positive group (P group) and composite group with high, middle and low doses (C1,C2,C3 group). Fed the positive group was given rosiglitazone maleate tablets(3.0 mg·kg-1), and composite group was given rosiglitazone maleate tablets(3.0 mg·kg-1) and ginsenosides Rb1 (60, 30, 15 mg·kg-1). Normal and model groups were given equal volume of saline. After two Morris water mazes, samples were collected and enzyme-linked immunosorbant assay (ELISA) method wasused to test plasma insulin and reverse transcription polymerase chain reaction (RT-PCR) was used to test 1-phosphatidylinosital 3-kinase(PI-3K )/p85. Result: Compared with normal group, model group used much longer time to find the plat(P<0.05), had more active plasma insulin and PI-3K/p85(P<0.05). Compared with control group,there was no significant differences in positive group,while plasma insulin and PI-3K/p85 were contrary in composite group(P<0.05). Conclusion: Ginsenosides Rb1 can improve the study and memory level of type 2 diabetes mice with cognitive impairment based on the reducing of plasma insulin, and the possible mechanism may reduce the level of PI-3K/p85.关键词：ginsenosides Rb1;2 diabetes memory impairment;plasma insulin level;PI-3K/p85;insulin signaling pathway
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect and mechanism of Shuwei decoction on gastrointestinal motility and gastrointestinal nerve-interstitial cells of Cajal-smooth muscle (ENS-ICC-SMC) network ultrastructure,to investigate in functional dyspepsia (functional dyspepsia, FD) with liver and spleen deficiency rats. Method: Seventy-two rats were randomly divided into normal group, model group,the low, middle and high dose groups of Shuwei decoction (7.67, 15.34, 30.68 g·kg-1), mosapride group (1.37 mg·kg-1),12 rats in each group.The model was made bychronic restraint stress combined with fatigue and improper diet. Three day after modeling corresponding drug,were give once daily for 14 days.The residual rate of stomach, small intestine propulsion rate were detected and to the structure of ENS-ICC-SMC network of gastric antrum and duodenum tissue were observed under electron microscope. Result: Compared with the control group, model group delayed gastric emptying, increased the gastric emptying rate, intestinal propulsion rate(P<0.05). Compared with the model group, treatment groups had significantly lower gastric emptying rate,and increased intestinal propulsion rate significantly (P<0.01). Compared with the model group, treatment groups improved ICC cells, ICC peripheral nerve endand relationship between ICC and ICC and between smooth muscle cells. Conclusion: Shuwei decoction can improve the relationship between the ultrastructure of ENS-ICC-SMC network, and improve the gastrointestinal motility, which is effective in the treatment of FD.关键词：Shuwei decoction;functional dyspepsia;gastrointestinal motility;gas-trointestinal nerve-interstitial cells of Cajal-smooth muscle network
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发布时间：2024-01-04 - 摘要：Objective: To study the protection action and possible mechanism of total flavonoids from Lycium barbarum(FLB) on the injured human umbilical vein endothelial cells(HUVEC)induced by hydrogen peroxide(H2O2). Method: The HUVEC oxidative stress damage model was established by using H2O2.This study was divided into as follows:normal group, 1 mmol·L-1 H2O2 injury group, FLB protective groups(100,200,400 mg·L-1)incubation for 24 hours and positive control (vitamin C 20 mg·L-1)group incubation for 24 h. MTT method was used to detect cell activity that was protected by FLB from damage caused by 1 mmol·L-1 H2O2.The levels of malondialdehyde (MDA)and superoxide dismutase (SOD),the activity of lactate dehydrogenase (LDH), the generation of nitric oxide(NO)in the supernatan were measured. Result: The levels of MDA and the activity of LDH were increased,the generation of No and the activity of SOD decreased obviously in H2O2 damage model group,the differences are significant (P<0.01).In FLB protection group, the activity of LDH and the levels of MDA were decreased, the generation of NO and the activity of SOD were increased obviously, the differences were significant(P<0.01). There was a dose dependent manner between the decreased degree of MDA, the activity of LDH, the degree of NO generation,the activity of SOD and FLB. Conclusion: FLB has the ability to protect vascular endothelial from damage caused by H2O2, its mechanism of the protecting of total FLB may be related to inhibiting lipid peroxidation damage cells, effectively improving the cell's antioxidant enzyme activity, eliminatimg free radicals, and promoting the production of NO.关键词：total flavonoids of Lycium barbarum;umbilical vein endothelial cells;oxidative damage
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发布时间：2024-01-04 - 摘要：Objective: To compare the effect of ethyl acetate fractions in Magnolia officinalis and ginger M. officinalis on gastrointestinal motility function. Method: SPF Kunming mice were randomly divided into high, medium and low dose group, positive and negative control group. The dosage for mice was 1,2 and 3 times the highest M. officinalis for adult. After 3 days of administration, the effect of gastrointestinal motility function was evaluated by the intestinal propulsion rate, ulceration caused by hydrochloric acid and contents of the gastrin. Result: There was significant difference between test groups and negative contrast groups in the intestinal propulsion rate (P<0.05), and no significant difference between test groups and positive contrast groups. There was significant difference between test groups and negative contrast groups on inhibition of gastric ulcer (P<0.05). There were significant difference between medium dosage of ginger M. officinalis, low dosage of M. officinalis and negative contrast group on contents of the gastrin (P<0.05). There was no significant difference between medium dosage of ginger M. officinalis and positive contrast groups. Conclusion: The ethyl acetate fractions extracted from M. officinalis and ginger M. officinalis may promote gastrointestinal motility and protect gastric mucosa. The effect of ginger M. officinalis is better than that of M. officinalis.关键词：Magnolia officinalis;ginger Magnolia officinalis;ethyl acetate fractions;gastrointestinal motility function;pharmacodynamic
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发布时间：2024-01-04 - 摘要：Objective: To study effect of Mieyou decoction on the expression of nuclear factor-κB(NF-κB) p65 protein and IL-8 in Helicobacter pylori(HP) related gastritis of spleen stomach damp heat syndrome mouse model. Method: Sixty BALB/c mice, half male and half female, were randomly divided into 6 groups:control group, model group, Mieyou decoction low, medium, high dose groups(6.2,12.4,24.8 g·kg-1), gastric triad group(0.28 g·kg-1). The model was established by high fat feeding and heat box in eleventh days, on sixteenth days with HP was inoculated, and serum HP antibody was detected using colloidal gold method,on thirty-sixth days corresponding drugs were given for 14 days, the other groups received normal feeding. All the mice were sacrificed on fifty-third days. The general condition of mice was observed, and the food and water intake, body weight, body temperature changes were obsedved. HE staining was used to observe the gastric mucosa and integral opticallens, the expression of NF-κB p65 protein was assayed by immunohistochmistry serum IL-8 was determined by ELISA. Result: Compared with control group, the model group increased antral gastric mucosa of acute and chronic inflammation score and expression of NF-κB p65 protein and IL-8 (P<0.05);compared with the model group,the Mieyou decoction middle and high dose group decreased gastric triad antral gastric mucosa of acute and chronic inflammation score and expression of NF-κB p65 protein and IL-8(P<0.05). Conclusion: Mieyou decoction may exert its anti-inflammatory effects through inhibition of NF-κB p65 protein expression and IL-8.关键词：Helicobacter pylori associated gastritis;Mieyou Decoction;spleen stomach damp heat syndrome;nuclear factor-κB p65 protein;interleukin-8
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of chrysophanol and donepezil on the ability of learning and memory and the activity of choline acetyltransterase (ChE), cholinesterase (ChAT), peroxidase (POD) and total antioxidant capacity (T-AOC) in the AD rat's brain, and then discuss the preventive and treatment effect of chrysophanol and donepezi on AD rat. Method: The AD model was produced by injecting D-galactose and AlCl3 intraperitoneally for 90 days. Rats were randomly divided into normal group, model group, chrysophanol group (0.14 mg·kg-1), donepezil group (0.33 mg·kg-1), chrysophanol joint donepezil groups (chrysophanol 0.14 mg·kg-1+donepezil 0.33 mg·kg-1, chrysophanol 0.07 mg·kg-1+donepezil 0.66 mg·kg-1, chrysophanol 0.28 mg·kg-1+donepezil 0.17 mg·kg-1). From the 20th day of D-galactose and AlCl3 intraperitoneal injection, chrysophanol and Donepezil were intragastric administrated respectively for 70 days. The learning and memory ability of each group rats were observed by Morris water maze. The activity of ChE, ChAT, POD and T-AOC in the AD rat's brain were determinated. Result: Rats intragastric administration with chrysophanol, donepezil and chrysophanol joint donepezil had shorter latency(P<0.05, P<0.01)and more numbers through the platform (P<0.05, P<0.01). Compared with chrysophanol group and donepezil group, rats in chrysophanol joint donepezil group (chrysophanol 0.28 mg·kg-1+donepezil 0.17 mg·kg-1) had shorter latency(P<0.05)and more numbers through the platform (P<0.05). Compared with the model group, the activity of ChAT, POD and T-AOC in the chrysophanol group, donepezil group and chrysophanol joint donepezil groups rat's brain elevated significantly(P<0.05), those of the chrysophanol joint donepezil group(chrysophanol 0.28 mg·kg-1+donepezil 0.17 mg·kg-1) elevated significantly(P<0.01). The activity of ChE reduced(P<0.05). Conclusion: Chrysophanol joint donepezil can improve the learning and memory ability of AD rats, their effects were collaborative. The effect is associated with their doses. Chrysophanol supplemented by donepezil may get a better effect on Alzheimer's disease.关键词：Alzheimer's disease;choline acetyltransterase;cholinesterase;total antioxidant capacity;peroxidase;chrysophanol;donepezil
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发布时间：2024-01-04 - 摘要：Objective: To study the possible link between preventing effect of atherosclerosis (AS) and tight connection(TJ)Buyang Huanwu Tang(BYHWT). Method: Seventy-two male ApoE-/-mice were randomly divided into five groups:model group, three dose groups of BYHWT(5,10,20 g·kg-1·d-1), Y-27632 group, and C57BL/6J mice was as a normal control group.AS model was established by intragastric administration of homocysteine(0.1 g·kg-1·d-1). Meanwhile,treatment groups were intragastrically administrated with BYHWT (5,10,20 g·kg-1·d-1),Y-27632 50 mg·kg-1·d-1. Eight weeks later, Western-blot and reverse transcription PCR(RT-PCR) were used to determine the expression of aortic claudin,junction adhesion molecule (JAM), zonula occludens (ZO). Result: Model group showed evident AS lesions.The expression of aortic claudin, JAM, ZO in the model group obviously decreased, compared with normal control group.Different doses of BYHWT could prevent atherosclerosis and up-regulate the claudin, JAM, ZO expressions. Conclusion: BYHWT could obviously increase the claudin, occludin,JAM, ZO expressions. BYHWT could prevent atherosclerosis induced by homocysteine via increasing the tight junction proteins expression.关键词：Buyang Huanwu Tang;homocysteine;atherosclerosis;tight junction
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects and its mechanism of Chaihu Shugan San(CHSGS) on hepatic fibrosis in rats. Method: The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum to rats.Seventy Wistar rats were divided into seven groups:blank group, model group, CHSGS high-dose group, mediate-dose group and low-dose group, prevention group, colchicin contrast group. Except the black groups, the other groups were injected with pig's serum without inactivation into abdominal cavity, twice per week and 0.5 mL each time, for 10 weeks. The prevention group was injected the stomach with the medicines(CHSGS 6.3 g·kg-1·d-1,10 weeks), the other treatment groups were treated as the prevention groups 6 weeks after modeling for 4 weeks. Then rats were randomly taken for decollation in six groups at the end of the 10th week:colchicin contrast group 0.5 mL·kg-1·d-1,CHSGS low-dose, mediate-dose and high-dose groups (3.5,6.3,12.6 g·kg-1·d-1).The changes of the serum biochemical indicator of the hepatic function and hepatic fibrosis were detected respectively. Result: Compared with model group,the serum content of alanine aminotransferase(ALT),aspartate aminotransferase (AST),r-glutamyl endopeptidase(GGT), alkaline phosphatase(ALP) and typeⅠ,Ⅲ,Ⅳcollage, hyaluronic acid (HA), laminin (LN) were depressed obviously in treatment groips,the content of hydroxyproline(HYP) in liver tissue was significantly lower in CHSGS mediate-dose group. Conclusion: Chaihu Shugan San has an effect on hepatic fibrosis model rats.关键词：Chaihu Shugan San;hepatic function;liver fibrosis;hydrocarbon proline
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发布时间：2024-01-04 - 摘要：Objective: To investigate the neuroprotective effects and mechanism of taxifolin against Aβ induced neural damage. Method: Primary neurons were isolated and purified from cerebral cortex of suckling mouse. The neurons were co-cultured with Aβ and taxifolin for 24 h. CCK8 method was used to test cell viability. The apoptosis was examined by Hoechst 33258 nuclear staining, Annexin V-FITC/PI double staining and caspase-3 activity. Result: In cell viability, compared with the normal group, cell viability of model group decreased significantly (49.2±1.3) %, different concentrations of taxifolin could improve the neuronal activity, the cell viability of 80 μmol·L-1 taxifolin increased to (68.7±3.2)%, compared with the model group. Compared with the normal group, the apoptosis rate of model group was (50.8±1.5)% (P<0.01), different concentrations of taxifolin could reduce the cell apoptosis rate, 40 μmol·L-1 taxifolin apoptosis rate was (41.5±2.7)%,compared with the model group (P<0.05). Compared with the normal group, in model group, caspase-3 activity was (2.37±0.16) U·μg-1(P<0.01), compared with the model group, 40 μmol·L-1 taxifolin group decreased the caspase-3 activity (1.77±0.07) U·μg-1(P<0.01). CCK8 assay displayed that taxifolin obviously increased cell viability. Hoechst 33258 nuclear staining and Annexin V-FITC/PI double staining showed that taxifolin could significantly decrease apoptotic rate compared with the Aβ treated group. Taxifolin also inhibited caspase-3 activity of neurons induced by Aβ. Conclusion: Taxifolin possesses the neuroprotective effects on Aβ damaged neurons, which may be associated with inhibition of caspase-3 activity in neurons to against apoptosis.关键词：taxifolin;neurons;β-amyloid peptide;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Ailuo Kechuanning on mitogen activatein kinase(p38MAPK),myeloperoxidase(MPO),interleukin-17(IL-17),C-reactive protein(CRP),and oxidative stress in rats with chronic obstructive pulmonary disease (COPD). Method: Lipopolysaccharide(LPS) smoke wasused to COPD rat model,the experimental animals were randomly divided into normal group,model group,Ailuo Kechuanning groups(7.75,15.52,31.04 g·kg-1·d-1),The activity of p-p38MAPK and MPO was detected by colorimetric method,enzyme-linked immunosorbent assay was used to determine the change of IL-17 and C-reactive protein(CRP) levels.the concentration of MDA, the activity of SOD and glutethione peroxiadase(GSH-Px) were measured in lung homogenates of all the groups. Result: The activity of p-p38MAPK and MPO, the levels of IL-17 and CRP were higher significantly,the activity of SOD and GSH-Px were decreased, the level of MDA was higher significantly in lung homogenates of the model group. Compared with the normal control group. While the activity of p-p38MAPK and MPO, and the levels of IL-17 and CRP was decreased significantly.the activity of SOD and GSH-Px were higher significantly, and the level of MDA was decreased significantly in lung homogenates of the rats treated with Ailuo Kechuanning(15.52 g·kg-1·d-1)compared with the model group. Conclusion: Ailuo Kechuanning may improve the pulmonary function of COPD. The effects of Ailuo Kechuanning for treatment of COPD may inhibite the activity of p-p38MAPK and MPO,and decrease the levels of IL-17, CRP and MDA, lead to the anti-inflammatory and against oxidative damage.关键词：chronic obstructive pulmonary disease;myeloperoxidase;interleukin-17;C-reactive protein;superoxide dismutase
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shugan Hewei decoction (SHD) on the expression of calcitonin gene related protein (CGRP) of medulla oblongata, spinal cord and gastric mucosa in depression model rats. Method: Totally 100 SPF Wistar rats (half male and female) were randomized into the normal group, model group, fluoxetine 0.36 mg·kg-1 group and the high and low dosage groups of SHD, with 20 in each group. The depression model was established with chronic unpredictable mild stress (CUMS) and separation for 4 weeks. The 3rd week after modeling each treatment group was given corresponding medicines. Fluoxetine group and high, low dosage(20,10 g·kg-1) of SHD group and were given for fourteen days. At the end of the fourth week, the internal fixation method was used to collect medulla oblongata vagus nerve nucleus tissue,spinal cord tissue (T6-T8) and gastric mucosa tissue. The method of immunohistochemistry (IHC) was used to detect the expression of CGRP in the above organizations. Result: Comparing with the normal group, the content of CGRP increased significantly in medulla oblongata,spinal cord and gastric mucosa of model rats(P<0.01). Compared with the model group, the content of CGRP reduced significantly in medulla oblongata,spinal cord and gastric mucosa of high, low dosage of SHD group and fluoxetine group(P<0.05, P<0.01).In addition, the effect of high dosage of SHD was equal to fluoxetine group in medulla oblongata and spinal cord, while the former was better than the later in gastric mucosa(P<0.05). Conclusion: SHD has regulation effects on the expression of CGRP in the signal path of brain-gut axis ‘medulla oblongata-spinal cord-gastric mucosa’, which may be part of the mechanism for relieving depression and improving gastrointestinal function.关键词：depression;medulla oblongata;spinal cord;gastric mucosa;Shugan Hewei decoction;calcitonin gene related protein
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective effects of Panax quinquefolius saponins (PQS) and to investigate its possible mechanism on myocardial ischemia-reperfusion model of rats. Method: Fifty rats were randomly divided into 5 groups:sham operation group, model group, low-and high-dose PQS groups (100, 300 mg·kg-1), and nimodipine group (21.6 mg·kg-1) of 10 rats each. The myocardial ischemia-reperfusion model was induced by ligature of coronary artery and reperfusion of rats except for rats in sham operation goup. All rats were ig administrated with corresponding medicines once daily for 15 days. The heart function and cardiac infarction area were observed. The contents of creatine phosphokinase (CPK), lactic dehydrogenase (LDH), tumor necrosis factor (TNF) -α and interleukin (IL) -6 in serum and superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and malonaldehyde (MDA) in cardiac muscle tissue were tested. Result: Compared with sham operation group, the left ventricular systolic pressure was increased, the left ventricular end-diastolic pressure was decreased, cardiac infarction area was increased, and the contents of CPK, LDH, MDA, TNF-α and IL-6 were decreased, the activities of SOD and GSH-Px were inhibited in model group (P<0.01,P<0.05). The above indexes were improved in the PQS groups (P<0.01, P<0.05). Conclusion: PQS can attenuate the damage caused by myocardial ischemia reperfusion and its mechanism may be involved with the anti-oxidation and anti-inflammatory activities.关键词：Panax quinquefolius saponins;ischemia reperfusion;anti-oxidation;anti-inflammatory
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Tongbian granules on the regulation of aquaporin 3 (AQP3) and aquaporin 8 (AQP8) in rats with slow transit constipation (STC), and to clarify its possible mechanism. Method: A slow constipation rat mode was induced by compound phenylethyl piperidine. The 50 model rats were randomly divided into model group, low-, medium-and high-dose Tongbian granules groups (9.3, 18.7, 37.3 g·kg-1), and polyethylene glycol powder group (2.1 g·kg-1). The another 10 normal rats were assigned to normal control group. All rats were ig adiminstrated with the corresponding medicines for 30 days.The body weight, 24-hour stool weight, the carbon powder propulsion in the colon of rats were recorded and measured. The distribution and relative content of AQP3 and AQP8 in rat colon were detected. Result: Compared with model group, the body weight, 24-hour stool weight, the carbon powder propulsion rate in the colon were decreased in model group. The above indexes were improved in the Tongbian granules groups (P<0.05). Immunohistochemistry results showed that the number of AQP3 and AQP8 were remarkably decreased in all dose Tongbian granules groups and polyethylene glycol powder group. Tongbian granules group of high dose had the best results (P<0.05). Conclusion: Tongbian granules has excellent curative effects in improving constipation defecation function of STC rats, which may be involved with the regulation of absorption and secretion of moisture achieved by depressing AQP3 and AQP8 in rat colon.关键词：Tongbian granules;slow transit constipation;aquaporin 3;aquaporin 8
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发布时间：2024-01-04 - 摘要：Objective: To establish mice model of functional dyspepsia (FD) and to investigate the effects of Weiyuanning on gastrointestinal motility and gastrointestinal hormones. Method: The mice were randomly divided into normal group, model group, low-, medium-and high-dose Weiyuanning groups (22.5, 45, 90 g·kg-1), and domperidone group (10 mg·kg-1). FD models were made by irregular food intake plus intraperitoneal injection with L-arginine in mice except for mice in normal group. All mice were ig administrated with corresponding medicines for 7 days. The body weights, the gastric remnant rate and small intestinal propulsion rate were measured. The gastric histopathological changes were observed. The contents of gastrin (GAS) and motilin (MTL) were detected by Elisa. Result: Compared with model group, the body weight, gastric emptying rate were decreased, the small intestinal propulsion rate was increased in model group (P<0.01). The above indexes were improved in the Weiyuanning groups (P<0.01). Meanwhile, the the contents of GAS in serum and MTL in plasma were increased in Weiyuanning groups (P<0.05, P<0.01). No significant changes were found in the improvement of gastric histopathological among all groups after the treatment. Conclusion: FD model could be successfully made by irregular food intake plus intraperitoneal injection with L-arginine. Weiyuanning has significant effect in improving indigestion in FD mice. Its mechanism may be related to elevating the levels of GAS in serum and MTL in plasma of mice.关键词：Weiyuanning;functional dyspepsia;gastrointestinal motility;gastrin;motilin
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant effect of Lycium ruthenicum buccal tablet in mice. Method: Fourty mice were randomly divided into 4 group:normal group (normal saline) low-and high-dose L. ruthenicum buccal tablet groups (0.08, 0.16 mg·g-1), and vitamin C group (0.02 mg·g-1). The mice were sacrificed by intragastric administration 28 days later and the total antioxidant capacity (T-AOC), the contents of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase enzyme (GSH-Px) and catalase (CAT) in serum and liver were determined using the test kits. Result: Compared with normal group, no significant changes were found in the mice body weight, while the contents of MDA in serum and liver was significantly decreased are (5.97±2.53),(11.08±3.72) μmol·L-1 and (5.84±1.44),(10.79±2.06) μmol·L-1(P<0.05,P<0.01), the activities of T-AOC, SOD, GSH-Px and CAT were significantly increased (P<0.05 or P<0.01) in high-and low-dose L. ruthenicum buccal tablet groups. Conclusion: L. ruthenicum buccal tablet has significant antioxidant activity in vivo.关键词：Lycium ruthenicum buccal tablet;peroxide;antioxidation
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of petroleum ether extract, n-butanol extract and Rubus spp seed oil from R. spp seed on lipid metabolism and lipid peroxidation of hyperlipidemia in mice. Method: One hundred and twenty mice were randomly divided into 12 groups:blank group, model group, positive control group, petroleum ether extract high dose group (800 mg·kg-1), medium dose group (400 mg·kg-1) and low dose group (200 mg·kg-1), n-butanol extract high dose group (600 mg·kg-1), medium dose group (300 mg·kg-1) and low dose group (150 mg·kg-1), R. spp seed oil high dose group (1 000 mg·kg-1), medium dose group (500 mg·kg-1) and low dose group (250 mg·kg-1). The mice hyperlipidemia model was established by ig high fat emul, the contents of triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA),and superoxide dismutase(SOD)activity in serum, and the coefficients of liver, kidney and spleen were tested. Result: Low dose group of petroleum ether extract could significantly decrease the contents of TG, TC, LDL-C and MDA and the coefficients of liver and spleen, and increase the content of HDL-C and the activity of SOD (P<0.05). Medium and low dose groups of n-butanol extract could significantly decrease the contents of TG, TC, LDL-C and MDA and the coefficients of liver and spleen and increase the activities of SOD(P<0.05). Medium dose group of R. spp seed oil could significantly decrease the contents of TG, TC and MDA and the coefficients of liver and spleen and increase the content of HDL-C and the activity of SOD(P<0.05). High dose group of R. spp seed oil could significantly decrease the contents of TG, TC and MDA and the coefficients of liver and spleen, and increase the activity of SOD (P<0.05). High dose group of n-butanol extract could significantly decrease the contents of TG and MDA and the coefficients of liver and spleen and increase the content of HDL-C and the activity of SOD (P<0.05). Low dose group of R. spp seed oil could significantly decrease the contents of TG, TC, LDL-C and the coefficients of liver and spleen and increase the content of HDL-C (P<0.05). High (TG and TC) and medium (TC, HDL-C), liver and spleen coefficients dose groups of petroleum ether extract were significant change. Compared with model group, other indicators had no significance, but there was a certain effect. Conclusion: Petroleum ether extract, n-butanol extract and R. spp seed oil had a certain degree of regulating lipid metabolism and lipid peroxidation. They could improve lipid accumulation in the liver and spleen of mice. It might be one of the possible mechanism of effective extracts of R. spp seed for reducing blood lipid.关键词：Rubus spp seed;hyperlipidemia;lipid metabolism;lipid peroxidation
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发布时间：2024-01-04 - 摘要：Objective: To compare the efects of Shangke Jiegu tablet and Jianbu Huqian pill on postoperative rehabilitation of patients with Lisfranc joint injury. Method: Sixty-eight Lisfranc joint injury patients were selected from October 2010 to May 2012 in our hospital. The patients were randomly divided into Shangke Jiegu tablet group and Jianbu Huqian pill group of 34 patients each. All patients were treated with open reduction and internal fixation surgery. The patients in Shangke Jiegu plate group received 4 Shangke Jiegu tablets three times daily. The patients in Jianbu Huqian pill group received 10 Jianbu Huqian pills twice daily. The postoperative evaluation of foot function, the time of clinical symptom improvement, callus X-ray scores at different time and adverse events of the Lisfranc joint injury patients in two groups were compared and analyzed. Result: Compared with Jianbu Huqian pill group, the overall excellent ratio of foot function was significantly increased (73.53%), the disappear time of clinical symptoms including swelling and tenderness (5.58±2.41) d and (10.64±2.43) d was reduced significantly in Shangke Jiegu plate group. The VAS score of (1.51±1.02) points was reduced significantly after treatment. The callus X-ray scores at 14, 21 d and 28 d were (0.78±0.09) points, (1.34±0.25) points, (2.51±0.42) points, respectively, which were significantly improved (P<0.05). Conclusion: Shangke Jiegu tablet could promote the efficacy of postoperative rehabilitation of Lisfranc joint injury. It could be an safe and effective medicine for adjuvant therapy and it is worthy to be applied widely in clinic.关键词：Shangke Jiegu tablet;Jianbu Huqian pill;Lisfranc joint injury;postoperative rehabilitation
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Yiqi Tongqiao decoction in the treatment of acute cerebral infarction (ACI), and determine its effect on serum peroxisome proliferator activated receptor gamma (PPAR-γ) level. Method: Seventy ACI patients were randomly divided into treatment group and observation group. Patients in two groups were given conventional treatment, and patients in the treatment group added Yiqi Tongqiao decoction. The patients in both groups received a 4-week period of treatment. Changes of China stroke scale (CSS) score, clinical symptom score and serum PPAR-γ level were observed before and after treatment. Result: ①Thirty patients in both groups completed the study. Clinical efficacy of the treatment group was 86.7%, which was higher than 73.3% of the observation group. ②Serum PPAR-γ in patients with ACI was obviously higher than that of the control group (P<0.01).③After treatment, clinical symptom score, CSS score and serum PPAR-γ level of two groups were significantly decreased (P<0.01), and the results in treatment group were better than those in the observation group (P<0.01). ④After treatment fasting plasma glucose(FBG),triglycerides (TG), total cholesterol (TC) of the treatment group decreased significantly (P<0.01),while the observation group had no significant change. Conclusion: Using conventional therapy plus Yiqi Tongqiao decoction have remarkable curative effect in treating ACI. Dynamic monitoring serum PPAR-γ level is conducive to assess the effect and progression in treating ACI.关键词：acute cerebral infarction;Yiqi Tongqiao decoction;peroxisome proliferator activated receptor gamma;neural function defect
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of budesonide combined with Feilike in children with asthma. Method: In eighty cases of patients admitted to our hospital were analyzed, and were randomly divided into two groups. The control group using budesonide treatment,experimental group combined with Feilike treatment, treatment effects were compared between the two groups. The experimental group after treatment, 3 cases of adverse reactions, adverse reaction rate of 7.5%, lower than that of the control group 22.5% (9/40)(P<0.05). Result: The ideal effect in treatment of experimental group was 95%, higher than that of the control group (80%) (P<0.05);the experimental group of 95% in our hospital for the overall satisfaction, higher than that of the control group(P<0.05);the experimental group cough relief time was (4.35±1.25) days, cough disappeared time was (8.99±2.17) days, the average treatment time for (17.12±1.5) days, lower than that of the control group (P<0.05);the experimental group after treatment for forced expiratory volume in one second(FEV1) index (1.57±0.31) L, peak expiratory flow (PEF) index (3.82±0.37) L, FEV1/forced vital capacity(FVC)index (83.54±0.62) %, higher than that of the control group (P<0.05). Conclusion: The higher incidence of asthma in children, mechanism of the disease is also more complex, incentives, and the lack of an ideal method to cure the disease, the clinical application of budesonide combined with Feilike ideal treatment effect, can effectively improve the symptoms of patients, is worthy to be popularized.关键词：budesonide combined with Feilike;asthma;clinical effect
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Liqi Qingre Fang by jejunal injection in combination with conventional symptomatic and supportive intervention in treating acute pancreatitis (AP). Method: Eighty patients with AP accorded with the inclusion criteria were randomly divided into two groups of 40 patients each. The patients in both groups received conventional symptomatic and supportive intervention and the patients in observation group added Liqi Qingre Fang by jejunal injection. The curative efficacy, relevant clinical indices and inflammatory factors were observed and compared. Result: The patients in observation group had a total therapeutic ratio of 92.5%, which was statistically higher than that of 70.0% in the control group (P<0.05). The disappearance time of abdominal pain, vomiting, fever, jaundice and the recovery time of serum amylase in the observation group were shorter than those in control group (P<0.05). After the treatment, inflammatory factors of interleukins (IL) -6, IL-8, tumor necrosis factor alpha(TNF-α) and high-sensitivity C-reactive protein(Hs-CRP) in the observation group were statistically lower than those in the control group (P<0.05). Conclusion: The effect of using Liqi Qingre Fang by jejunal injection in combination with conventional symptomatic and supportive intervention is reliable in treating AP. It could increase the toal therapeutic ratio, improve relevant indices and alleviate inflammatory reaction, which is worthy to be applied widely in clinic.关键词：Liqi Qingre Fang;conventional symptomatic and supportive intervention;jejunal injection;acute pancreatitis;inflammatory factor
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发布时间：2024-01-04 -
Fuzheng Xiaoliu Decoction Combined with Paclitaxel in Treating Patients with Advanced Ovarian Cancer
摘要：Objective: To investigate the clinical effect of Fuzheng Xiaoliu decoction combined with paclitaxel in the treatment of advanced ovarian cancer. Method: Seventy-seven patients were divided into control group and treatment group according to their admission date (single or double date). The patients in control group received conventional treatment, and the patients in treatment group received Fuzheng Xiaoliu decoction plus paclitaxel. The changes of clinical symptoms, tumor were compared between two groups before and after treatment.The occurrence of adverse reactions were monitored. Result: Eleven patients achieved complete remission in control group, 19 patients had partial remission, and 9 patients had stable disease in this study. The total effective rate in treatment group was 69.8%, which was higher than that in control group (44.1%, P<0.05). The operation time, the volume of ascites and hemorrhage in treatment group was (215.34±32.76) min, (678.35±32.32) mL and (502.89±118.53) mL, respectively, in, treatment group, which were less than that in control group (P<0.05).The incidence rate of adverse reaction was 44.19% in treatment group and 58.82% in control group with no significant difference. Conclusion: Fuzheng Xiaoliu decoction combined with paclitaxel have curative effect in treating patients with advanced ovarian cancer, which is worthy to be applied widely in clinic.关键词：Fuzheng Xiaoliu decoction;taxol;advanced ovarian cancer- 2
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发布时间：2024-01-04 - 摘要：Objective: To observe clinical effect and mechanism of Tangshenbao granules combined with enalapril maleate tablets in treating early diabetic nephropathy (DN). Method: Eighty patients with DN were randomly divided into Contrast group (40 cases) and joint group (40 cases) by random number table. Blood glucose of patients in both groups was controlled strictly and blood pressure was controlled by oral 5-10 mg amlodipine tablets once daily. Patients in Contrast group received enalapril maleate tablets, 10 mg/time, 1time/day. Based on the treatment of Contrast group, patients in joint group received 1 dose formula granules of Tangshenbao granules in twice daily. Patients in two groups received a 12-week period of treatment. The ratio of microalbuminuria and creatinine (mALB/Ucr), urine albumin excretion ratio(UAER), fasting blood-glucose (FBG), glycosylated hemoglobin (HbAlc), serous cystatin-c (Cys-C), haematic β2 microglobulin (β2-MG), N-acetyl-β-D-glucosaminidase (NAG) and kidney injury molecule-1(KIM-1) were detected before and after treatment. Result: After treatment, UAER and mALB/Ucr in joint group were lower than those in Contrast group (P<0.01). Compared with the time before treatment, levels of FPG and HbAlc in both groups were decreased(P<0.01) and level of HbAlc in joint group was lower than that in Contrast group (P<0.05). Compared with the time before treatment, levels of Cys-C, β2-MG, NAG and KIM-1 in Contrast group were decreased and thay were lower than those in joint group (P<0.01). The curative efficacy in Contrast group was superior to that in joint group according to Ridit analysis (P<0.05). Conclusion: Tangshenbao granules combined with enalapril maleate tablets has a good protection in treating early DN and its curative efficacy is superior to enalapril maleate tablets therapy alone.关键词：early diabetic nephropathy;Tangshenbao granules;serous cystatin-c;β2 microglobulin;N-acetyl-β-D-glucosaminidase;kidney injury molecule-1
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect and the action mechanism of Guishao Shangjin decoction by oral administration combined Tongluo Zhitong powder by external application in treating patients with acute lumbar sprain (ALS). Method: Ninety patients were randomly divided into control group (45 cases) and observation group (45cases) by random number table. Patients in control group took oral 0.2 g lbuprofen tablets 3 times daily plus dichofenac diethylammon by external application. Patients in observation group took oral Guishao Shangjin decoction and Tongluo Zhitong powder by external application. Patients in two groups received a 5-day period of treatment. The pain, tenderness, swelling and ecchymosis scores were recorded. The waist movement obstacles and waist dysfunction scores were conducted. The change of blood rheology, β-endorphin (β-EP), thromboxane B2 (TXB2), 6-Ketone prostaglandin Fla (6-Keto-PGFla) were detected before and after treatment. Result: The clinical curative effect in observation group was superior to that incontrol group through Ridit analysis (P<0.05). Scores of pain, tenderness, swelling and ecchymosis in observation group were lower than those in control group (P<0.01). Scores of waist movement obstacles and waist dysfunction in observation group were lower than those in control group (P<0.01). Improvement of blood rheology indexes including whole blood viscosity (high shear, low shear), plasma viscosity, fibrinogen and platelet aggregation rate in observation group were superior to those in control group (P<0.01). After treatment, levels of β-EP and 6-Keto-PGFlα of observation group were higher than those in control group (P<0.01), while the TXB2 level was lower than that in control group (P<0.01). Conclusion: Guishao Shangjin decoction by oral administration combined Tongluo Zhitong powder by external application could promote blood circulation, reduce inflammatory reaction, and improve level of β-EP in patients with ALS. It has a good analgesia and detumescence effect, and coud enhance the recovery of waist activity function.关键词：acute lumbar sprain;Guishao Shangjin decoction;Tongluo Zhitong powder;blood rheology;thromboxane B2;prostaglandin;β-endorphin
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发布时间：2024-01-04 - 摘要：Objective: To discuss the short-term curative efficacy of Liangxue Xiaoyin decoction in treating psoriasis vulgaris at active stage (blood heat syndrome) and its adjusting function on T-cell subset in peripheral blood and inflammatory factor. Method: Ninety-five patients were randomly divided into control group (46 cases) and observation group (49 cases) by random number table. Patients in both groups received tazarotene gel by external use, and patients in control group added 4 Fufang Qingdai capsules 3 times daily, patients in observation group added dialectical therapy of Liangxue Xiaoyin decoction 1 dose daily. Patients in two groups received a 8 week period of treatment. Scores of lesion size of skin, psoriasis area and severity index (PASI) and itch degree were graded by visual analogue scale (VAS) before and after 2, 4 and 8-week treatment. Scores of dermatology life quality index (DLQI) and teat in blood system were graded, and levels of T-cell subset in peripheral blood and serous interleukin-6 (IL-6), interleukin-17 (IL-17) and tumor necrosis factor-α(TNF-α) were detected before and after treatment. Result: The clinical effect in observation group was superior to that in control group by Ridit analysis (P<0.05). Score of PASI in observation group was lower than that in control group after 8-week treatment (P<0.01), and VAS score of itch degree in observation group was lower than that in control group after 4 and 8-week treatment (P<0.01). Scores of DLQI and heat in blood system in observation group were lower than those in control group (P<0.01). Levels of CD3+,CD4+,CD4+/CD8+ in both groups were increased as compared with those before treatment, while the observation group gained higher results (P<0.01). Level of CD8+ in both group was decreased as compared with that before treatment, while the observation group gained lower result (P<0.01). After treatment, levels of serum IL-6, IL-17 and TNF-α in observation group were inferior to those in control group (P<0.01). Result: Using Liangxue Xiaoyin decoction orally combined Tazarotene gel externally could control psoriasis vulgaris during active stage (blood heat syndrome) patients with psoriasis skin lesions and stabilize the disease situation. The mechanism may be related to regulating imbalance of peripheral blood T cell subsets and reducing proinflammatory medium.关键词：psoriasis vulgaris;blood heat syndrome;Liangxue Xiaoyin decoction;T cell subsets;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: To discuss the curative efficacy of Shugan Tiaoxin decoction and its influence to on serous inflammatory factors in treating malignant tumor combined with depression. Method: One hundred and ten eligible patients were randomly divided into control group (55 cases) and observation group (55 cases) by random number table. Patients in control group received Fluoxetine capsules (starting dose was 20 mg/day, taken after breakfast, and the dose was adjusted according to patients' state after 2 weeks). Based on the treatment of control group, patients in observation group added 1 dose Shugan Tiaoxin decoction daily. Patients in two groups received a 8-week period of treatment. Before and after treatment, the Hamilton depression rating scale (HAMD) and the self-rating depression scale were graded. The quality of life scale (QLQ-BR30) of European Organization for Research and Treatment of Cancer (EORTC) was graded. Levels of serous interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected. Result: The depressant action in observation group was superior to that in control group (P<0.05), and depression symptom in observation group was better than that in control group(P<0.01), and scores of HAMD and SDS in observation group were lower than those in control group (P<0.01). Scores of role function, emotional function and global quality of life in observation group were all higher than those in control group (P<0.05, P<0.01). Scores of pain, insomnia, appetite loss, constipation and diarrhea in observation group were lower than those in control group (P<0.05, P<0.01). Levels of IL-1β,IL-6 and TNF-α in observation group were lower than those in control group, and level of IL-2 was higher than that in control group (P<0.01). Conclusion: Shugan Tiaoxin decoction could ameliorate depression symptom, reduce symptoms of depression and improve patients' quality of life in treating malignant tumor combined with depression. Its mechanism of action may be related to the regulation of serous inflammatory factors.关键词：malignant tumor;depression;Shugan Tiaoxin decoction;inflammatory factors
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative efficacy of Xiaopi Hewei capsules in treating precancerous lesions of gastric carcinoma (PLGC) which belonged to stagnation of liver-qi and stomach-qi, and to investigate its influence on endothelin (ET-1), gastrin (GAS) and somatostatin (SST). Method: Sixty-four patients with PLGC were randomly divided into control group (32 cases) and observation group (32 cases) by random number table. Patients in both groups received 20 mg omeprazole magnesium enteric-coated tablets,2 bismuth potassium citrate tablets and 0.25 g clarithromycin tablet twice daily for 2 weeks. Patients in control group added 4 weifu Chun tablets thrice daily, and patients in observation group added 6 Xiaopi Hewei capsules thrice daily for 6 months. Scores of stagnation of liver-qi and stomach-qi were evaluated. Gastroscope and pathology were carried out and scores of atrophy of gastric mucous, degree of intestinal metaplasia and dysplasia were graded. Helicobacter pylori (HP) was detected. The levels of ET-1, GAS and SST were detected before and after treatment. Result: By ordinal data chi-square test, the results of gastroscopy and pathological in observation group were superior to those in control group (P<0.05). Improvement of gastric mucosa atrophy in both groups was better than before according to chi-square test (P<0.01), while the observation group obtained better results (P<0.05). Liver-qi stagnation syndrome score in observation group was less than that in control group at the third and sixth months after treatment (P<0.01). Scores of intestinal metaplasia or (and) dysplasia in observation group were less than those in control group (P<0.01). Clearance of HP in control group was 79.2%, while the data in observation group was 84%. There was no significant difference between both groups. Infection rate of HP in control group was 40%, while the data in observation group was 17.14%. Infection rate in observation group was lower than that in control group after 6-month treatment (P<0.05). Levels of serum ET-1 and GAS in observation group were less than those in control group, SST level in observation group was higher than those in control group (P<0.01). Conclusion: Xiaopi Hewei capsules could improve clinical symptoms with PLGC patients (liver-qi stagnation syndrome), reduce the recurrence of HP. It has certain blocking effect on the progression from PLGC to gastric cancer. Its mechanism may be related to enhancing the serum level of SST and reducing the serum levels of ET-1 and GAS.关键词：precancerous lesions of gastric cancer;Xiaopi Hewei capsules;endothelin;gastric secrete element;somatostatin;helicobacter pylori
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发布时间：2024-01-04 - 摘要：Rare books of traditional Chinese medicine recorded a wealth of medical and cultural knowledge for descendants. Among them, a lot of the plant drawing is an excellent heritage of Chinese medicine in herbal literatures. It is the main target for textual research. Morindae Officinalis Radix was first named in the and it has been used as a common herbal in chinese medicine. As a famous export commodity, it is named one of the ‘Four Southern Medicine Herbs’. At present, research scholars believe that the ancient and the modern Morindae Officinalis Radix come from different plant species, and it is without a clear conclusion for the sources of Morindae Officinalis Radix. It considers that ancient Morindae Officinalis Radix is derived from Tetrastigma hemsleyanum and Tupistra in the textual research.关键词：Morindae Officinalis Radix;textual research;Tetrastigma hemsleyanum;Campylandra chinensis
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发布时间：2024-01-04
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