最新刊期
卷 21 ， 期 1 ， 2015
- 摘要：In recent years, study of functional genes has become a hot area of research in medicinal plants. Research contents include gene cloning, characterization, polymorphism analysis, expression pattern analysis and relationship analysis between functional genes and metabolic pathway. The roots of Glycyrrhiza uralensis are widely used in China. Besides the medicinal value, they are also used as industrial raw materials and tobacco additives. Among all of the natural active components glycyrrhizic acid is believed to be the marker compound to characterize the quality of this Chinese herb. Many studies have shown that glycyrrhizic acid possesses various biological activities, such as anti-inflammatory, antiturmor and immune-stimulating activities. In this paper the cloning, characterization and polymorphism of functional genes involved in glycyrrhizic acid biosynthesis in G. uralensis were analyzed. This work is significant for further studies concerned with strengthening the efficacy of G. uralensis by means of improved glycyrrhizic acid content and providing research ideas for functional gene studies in other medicinal plants.关键词：medicinal plants;glycyrrhizic acid;functional genes;3-hydroxy-3methylglutary CoA reductase;squalene synthase;β-amyrin synthase;cytochrome P450 monooxygenase
- 3
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To investigate role of accessories in processing of Rehmanniae Radix Praeparata with Jianchang band and to offer reference for illustrating its processing mechanisms. Method: Fingerprints of Rehmanniae Radix Praeparata was determined by HPLC,mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-12 min,5%-10%A;12-40 min,10%-15%A;40-70 min,15%-25%A;70-85 min,25%-28%A) and detection wavelength was 320 nm,chromatogram difference of processed products was evaluated by 2004 A version of‘Chinese medicine fingerprint similarity evaluation system’. Result: There were obvious differences between Rehmanniae Radix and Rehmanniae Radix Praeparata.In contrast to Wenzhi Rehmanniae Radix Praeparata,similarity values of rice wine negative contrast,Amomi Fructus negative contrast,Citri Reticulatae Pericarpium negative contrast,Amomi Fructus and Citri Reticulatae Pericarpium negative contrast,Amomi Fructus and Citri Reticulatae Pericarpium and rice wine negative contrast were 0.844,0.741,0.727,0.717,0.717,respectively. Conclusion: This method is simple,accurate and reproducible.It demonstrates that new components are produced or contents of known components are changed due to accessories during processing of Rehmanniae Radix Praeparata.关键词：Jianchang band;Rehmanniae Radix Praeparata;fingerprint;similarity evaluation;processing mechanism
- 4
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To study on re-evaluation of extraction process of Qingnao Fushen liquids,to provide experimental basis for improving industrial production of this preparation. Method: Determination of baicalin by HPLC,mobile phase of methanol-0.37% phosphoric acid(47:53),detection wavelength of 280 nm.Taking composite score of amounts of dry extract and baicalin as indexes,orthogonal test was adopted to optimize percolation process with the amount of solvent,percolation velocity,ethanol concentration as factors.Physical and chemical properties of different extracts were compared through ultraviolet scanning,near infrared scanning and infrared scanning. Result: Optimum extraction condition was as following:percolated by 8 times the amount of 40% ethanol at 2.27 mL·min-1·kg-1;amounts of dry extract and baicalin were 21.64 g and 31.23 mg.Ultraviolet full wavelength scanning,near infrared scanning,infrared scanning and dry extract amount of percolate and immersion fluid were most the same. Conclusion: Effective ingredients extracted from these two kinds of extraction process are all most the same,so percolation process can substitute immersion method.关键词：Qingnao Fushen liquids;immersion method;percolation;baicalin
- 3
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: Developing a method for detecting benzene series residues from HPD-300 macroporous resin for Chuanxiong Rhizoma extract,to provide a reference for promotion of macroporous resin. Method: Chuanxiong Rhizoma extract was dissolved by dimethyl sulfoxide,headspace sampling method was adopted,residues of organic solvent in macroporous resin was determined by capillary gas chromatography,column was temperature-programmed(initially heated at 50℃ for 10 min;rose to 70℃ with a speed of 5℃·min-1,1asting for 16 min;then rose to 120℃ with a speed of 5℃·min-1,1asting for 10 min),inlet temperature was 220℃,split ratio was 10:1,pressure was 58.799 kPa,total flow was 10.772 mL·min-1,H2 flow was 40 mL·min-1,air flow was 300 mL·min-1,make-up(N2) flow was 30 mL·min-1,carrier gas(N2) flow was 20 mL·min-1.Headspace conditions were:sample temperature at 80℃,manifold temperature at 100℃,pipe temperature at 110℃,balance time of 30 min,sampling interval of 55 min and sample quantity of 1 mL. Result: Linear relations between peak area and mass concentration of seven residues were good with r>0.999,average recoveries of n-hexane,cyclohexane,benzene,toluene,xylene,styrene,divinyl-benzene were 99.2%,97.3%,103.4%,98.7%,97.0%,101.9% and 98.5%,respectively. Conclusion: This method can be used for detecting benzene series residues in HPD-300 macroporous resin.关键词：macroporous adsorption resin;residues;headspace-GC;Chuanxiong Rhizoma extract
- 3
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of rheumatism ostalgia alcohols and establish its quality standard. Method: HPLC was adopted to determine the content of curcumin with mobile phase consisting of 4% glacial acetic acid-acetonitrile(52:48) and detection wavelength at 430 nm.With composite score of amounts of general solid and curcumin as index,orthogonal design was employed to optimize extraction technology of rheumatism ostalgia alcohols with soaking time,ethanol concentration and amount as factors.Angelicae Sinensis Radix,Ventilago Leiocarpae Radix et Caulis,Curcumae Longae Rhizoma and Acanthopanacis Cortex were identified by TLC. Result: Curcumin showed a good linear relationship at a range of 0.021 2-0.106 μg with r of 0.999 8 and average recovery of 97.81%.Optimal extracting technology was:soaked 30 days with 10 times the amount of 50% ethanol.TLC spots were clear and the blank test showed no interference. Conclusion: This technology is stable feasible with high extraction amount of index ingredient,which is available for industrial production of rheumatism ostalgia alcohols.This established TLC can control quality of this preparation effectively.关键词：Yao medicine;rheumatism ostalgia alcohols;curcumin;quality standard
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of ginsenosides from flowers of Ginseng Radix et Rhizoma. Method: UV was adopted to determine the content of total saponins with ginsenoside Re as index ingredient and detection wavelength at 550 nm.Taking extracting amount of total saponins as index,orthogonal test was adopted to optimize extraction technology of ginsenosides with material-liquid ratio,ethanol concentration,extraction time and times as factors.The content of ginsenosides was determined by UV spectrophotometry. Result: Optimal extraction conditions were as follows:extracted thrice with 10 times the amount of 80% ethanol at 85℃,1 h for each time;yield of total saponins was 18.66%. Conclusion: This optimized extraction process is stable and reasonable with high yield of active constituents,it can provide a reference for development and utilization of flower resource of Ginseng Radix et Rhizoma.关键词：Ginseng Radix et Rhizoma flowers;total saponins;Ginsenoside Re;extraction process
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate change of ginsenosides before and after co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma by ultra-flow liquid chromatography couple with quadrupole-time-of-flight mass-spectrometry(UFLC/Q-TOF-MS) and principal component analysis(PCA). Method: Chromatographic separation was performed on an ACQUITY UPLC T3 C18 column with a gradient elution of water-acetonitrile(containing 0.3% formic acid).Mass spectrometer equipped with electrospray ionization source was used as detector under negative ion mode.Difference of co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma,Ginseng Radix et Rhizoma decoction,Coptidis Rhizoma decoction(blank) were classified by PCA with Markerview 1.2.1 and other softwares. Result: Ten very different compound in three groups were found,contents of oleanolic acid and two unknown compounds significantly increased,while contents of 20-glucose-Rf,ginsenoside Rf,Ra3,Rb1,Ra2,Rb3,Rs2 significantly decreased. Conclusion: Samples of three groups are well classified by PCA in negative ion mode,it indicates that deglycosylation of ginsenosides may be material basis of co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma.关键词：UFLC/Q-TOF-MS;Ginseng Radix et Rhizoma;Coptidis Rhizoma;ginsenosides
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of total flavonoids from Abelmoschi Corolla with macroporous resin. Method: Five kinds of resins for purification of total flavonoids from Abelmoschi Corolla were compared by static adsorption and elution test.UV was employed to determine the content of total flavonoids with detection wavelength at 510 nm;based on single factor tests,taking composite score of quality and purity of total flavonoids as index,orthogonal design were used to optimize purification process conditions with ethanol concentration,concentration and pH of sample solution as factors. Result: AB-8 macroporous resin was selected,optimum technological conditions were as follows:ratio of diameter-height 1:6,sample solution concentration of 0.2 g·mL-1,pH 5.0,volume of sample solution 3 BV,with 6 BV of 75% ethanol as eluant;quality and purity of total flavonoids were 417.5 mg and 58.04%. Conclusion: Total flavonoids from Abelmoschi Corolla can be effectively purificated and separated by AB-8 macroporous resin.关键词：Abelmoschi Corolla;total flavonoids;macroporous resin;purification process
- 3
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To optimize inclusion process of volatile oil in Shidan granules,in order to provide a reference for clinical application and promotion of this preparation. Method: With inclusion rate of volatile oil and inclusion yield as comprehensive evaluation index,effects of saturated water solution and trituration method on inclusion were compared by single factor tests.Orthogonal design was used to optimize inclusion technology with ratio of volatile oil to β-cyclodextrin(β-CD),the amount of water and grinding time as factors,inclusion complex of volatile oil were detected by TLC,UV and microscopic imaging,then its stability was investigated. Result: Trituration method was adopted,the best inclusion conditions were as follows:β-CD-volatile oil(8:1),triturated 3 hours with 3 times the amount of water;encapsulation efficiency of volatile oil was 87.04% and yield of inclusion complex was 89.12%.Volatile oil retention rates of β-CD inclusion complex in the zeroth,first,third day were 100%,89.23% and 78.46%. Conclusion: Volatile oil is packed into cavity of β-CD and relatively stable β-CD inclusion complex is formed,stability of Shidan granules significantly increases.This optimized technology is stable and feasible,which is suitable for large-scale production of this preparation.关键词：Shidan granules;volatile oil;inclusion;β-cyclodextrin;trituration method
- 3
- |
- 3
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Natrii Sulfas. Method: With the content of magnesium ion in Natrii Sulfas as index,orthogonal test was adopted to optimize processing technology by taking radish addition,water addition and processing time as factors.The content of magnesium ion was determined by atomic absorption spectrophotometry with detection wavelength of 285.28 nm.The content of sodium sulfate in different processed products was measured. Result: The best processing technology was as following:added 5 times the amount of water and 0.1 times the amount of radish for processing 1 hour.Contents of magnesium ion and sodium sulfate were 7.143 μg·g-1 and 97.83%,respectively. Conclusion: This optimized processing technology is stable and feasible,it is significant to regulate processing technology of Natrii Sulfas,this paper shows that Natrii Sulfas processed by radish is rational.关键词：Natrii Sulfas;processing technology;magnesium ion;atomic absorption spectrophotomery;sodium sulfate
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Lianshen granules,in order to provide a reference for development of this preparation. Method: With yield of volatile oil as index,single factor tests were adopted to investigate effects of the amount of water,soaking time and distillation time on extraction technology of volatile oil in Angelicae Sinensis Radix,Cinnamomi Cortex and Magnoliae Flos.UV was adopted to determine the content of total alkaloids with detection wavelength of 350 nm.The content of berberine hydrochloride was determined by HPLC,which used acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate(50:50) as mobile phase,detection wavelength was 345 nm.With contents of berberine hydrochloride and total alkaloids,yield of dry extract as comprehensive evaluation index,effects of the amount of water,extraction time and times on water extraction technology of Lianshen granules was investigated by orthogonal test. Result: Optimum extraction technology of volatile oil was as following:soaked one hour with eight times the amount of water,then distilled five hours.Optimum water extraction technology conditions were as follows:boiled herbs with eight times the amount of water for thrice,one hour for each time.Dry extract yield was 25.91%,extraction amounts of total alkaloids and berberine hydrochloride were 512.34,130.81 mg,respectively. Conclusion: Optimized extraction technology is simple,stable and feasible,which is suitable for industrial production of Lianshen granules.关键词：volatile oil;total alkaloids;berberine hydrochloride;Lianshen granules
- 5
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare contents of volatile oil and artemisinin in different plant tissues of Artemisia annua,in order to provide experimental basis for full utilization of this herbs resources. Method: Volatile oil was extracted according to 2010 edition of ‘Chinese Pharmacopoeia’.The content of artemisinin in leaves,flowers,roots,stems and fruits of A. annua was determined by HPLC,mobile phase was methanol-phosphate buffer solution(60:40),flow rate was 0.8 mL·min-1 and detection wavelength set at 260 nm. Result: The content of volatile oil in various tissues of A. annua was in the order of flowers >leaves >fruits >roots >stems,but the content of artemisinin was flowers >fruits >leaves >roots >stems.Artemisinin showed a good linear relationship in 1.025×10-3-1.025 μg with r of 1.0,average recovery was 100.34% with RSD of 2.3%. Conclusion: Distributions of volatile oil and artemisinin in different tissues of A. annua have large differences,but they both have the highest content in flowers.This established determination of artemisinin is accurate,stable and reliable,which can be used for quality control of A. annua.关键词：Artemisia annua;volatile oil;artemisinin;pre-column derivatization
- 3
- |
- 4
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of wine Paeoniae Radix Alba and provide a reference for clinical medication of this herb. Method: HPLC was employed to determine the content of paeoniflorin with mobile phase of acetonitrile-0.1% phosphoric acid(14:86) and detection wavelength of 230 nm.Taking appearance,contents of paeoniflorin and water-soluble extract as comprehensive evaluation index,orthogonal test was adopted to investigate effects of the dosage of yellow rice wine,temperature and time on processing technology. Result: The best processing conditions were 10% dosage of yellow rice wine,processing temperature of 90℃ and processing time of 15 min.Wine Paeoniae Radix Alba was slightly yellowish with slightly wine aroma,mass fractions of paeoniflorin and water-soluble extract were 3.79% and 37.51%,respectively. Conclusion: Appearance is an important index for evaluation of quality criteria of Chinese medicine pieces.This optimized processing technology is stable,feasible and applicable to industrial production of wine Paeoniae Radix Alba through comprehensive scoring method.关键词：Paeoniae Radix Alba processed with wine;paeoniflorin;water-soluble extract;appearance;composite score
- 3
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To compare content differences of main components in volatile oil from Zhizhu pills extracted in light and dark conditions. Method: Volatile oil in Zhizhu pills was obtained by steam distillation,one extracting volatile oil device was not wrapped to get oil exposed to the sun light oil(light condition oil) and the another device was wrapped with black cloth to get oil keeping out of the sun light(dark condition oil),these two devices were used to extract volatile oil for the same time.Comparison of contents of atractylone,atractylenolide Ⅰ,Ⅱ,Ⅲ in these two kinds of volatile oil from Zhizhu pills were determined by HPLC,mobile phase consisted of water(A)-acetonitrile(B) for gradient elution of (0-22 min,55%-64%B;22-28 min,64%B;28-48 min,64%-100%B),detection wavelength were 220 nm and 275 nm. Result: Yields of volatile oils extracted in dark and light conditions were 1.05% and 1.03%,respectively.The content of atractylone in light condition oil was significantly lower than that in dark condition oil,which were 73.01 mg·g-1 and 100.3 mg·g-1,respectively.The content of atractylenolide Ⅰ in light condition oil was much higher than that in dark condition oil,which were 0.757 2 mg·g-1 and 0.354 1 mg·g-1,respectively.There was no significant difference in the content of atractylenolide Ⅱ between these two kinds of oils,which were 2.026 mg·g-1 and 2.158 mg·g-1,respectively.The content of atractylenolide Ⅲ in light condition oil was obviously higher than that in dark condition oil,which were 0.412 4 mg·g-1 and 0.321 9 mg·g-1,respectively. Conclusion: No significant differences of yields of volatile oil are existed in these two extracted methods,but main ingredients are markedly different.It is suggested that inherent compounds change caused by experimental process should be avoided in constituent studies of Chinese prescription.关键词：Zhizhu pills;volatile oil;illumination;Atractylodis Macrocephalae Rhizoma;Aurantii Fructus Immaturus;atractylone;atractylenolides
- 3
- |
- 4
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To analyze the feasibility of using Near-infrared (NIR) spectroscopy for quality control of liquid-liquid extraction procedure of Cardeniae Fructus in the preparation of reduning injection. Method: 147 extraction stream samples from seven batches were collected and NIR off-line spectra were acquired by Luminar 5030 spectrometer. The contents of geniposide (GS) and soluble solid content (SSC) were determined by the reference method. The partial least square(PLS) algorithm was used to build GS and SSC NIR models and predict the contents in prediction set. Result: The R2 of the established GS and SSC models were 0.987 2 and 0.994 7 as well as the RMSEC was 1.460 9 and 2.367 7, which indicated the models had good performance. Besides, the predictive results of 20 samples were satisfactory. The R2 was 0.980 7 and 0.986 1, the RMSEP was 1.827 5 and 7.307 7 and the RSEP was 3.08% and 5.29% for GS and SSC, respectively. Both of the RSEP values were lower than 6%, which can satisfy the quality control standard in the traditional Chinese medicine production process. Conclusion: The established models could precisely predict the content of GS and SSC. The results demonstrated that the NIR spectroscopy might be a suitable and alternative technology for monitoring the liquid-liquid extraction procedure of Gardeniae Fructus.
- 3
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To identify the alkaloid compounds of Aconite Lateralis Radix and to probe into the dynamic changing regularities of main constituents during decoction process. Method: HPLC-Q-TOF-MS was used to identify chemical constituents and to analysis the content changes of main constituents during decoction process using dissolution rate. Result: Totally 36 alkaloid compounds of Aconite Lateralis Radix were identified and the dissolution rates of 25 compounds were determined in the process of decoction. The results showed that diester alkaloids (mesaconitine,hypaconitine,aconitine and deoxyaconitine) decreased quickly and their content were not detected after decocting 2 h;monoester alkaloids (benzoylaconine,benzoylmeaconine,benzoylhypaconine,10-OH benzoylmeacinine,benzoyldeoxyaconine and dehydrated-benzoylhypaconine) firstly increased and reached the peak in 6 to 8 h and then gradually deceased. Aconine alkaloids (mesaconine,aconine and hypaconine) increased all the time during decoction process. General alkaloids firstly increased and reached to the peak in 6 to 8 h and then tended to stability. Conclusion: The analysis of the alkaloid compounds of Aconite Lateralis Radix was comparatively comprehensive and the dynamic changing regularities of the alkaloid compounds were explored. It provided a reference for quality control and clinic application of Aconite lateralis Radix.关键词：Aconite Lateralis Radix;alkaloid;HPLC-Q-TOF-MS;constituent analysis;decoction;changing regularities
- 3
- |
- 3
- |
- 12
发布时间：2024-01-04 - 摘要：Objective: To establish an ultra fast liquid chromatography (UFLC) method for simultaneous determination of alisol C 23-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B and alisol B 23-acetate in the Alismatis Rhizoma. Method: The seperation was performed on an Ultimate UFLC-AQ C18 column (2.1 mm×100 mm, 3 μm) using gradient elution with the mobile phase consisting of acetonitrile and water at a flow rate of 0.3 mL·min-1. The column temperature was maintained at 30℃, and the DAD detection wavelengths were set at 208 nm and 245 nm. Result: The linear range of alisol C 23-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B and alisol B 23-acetate were 0.179 0-17.88(r=0.999 8), 0.500 0-100.0(r=0.999 7), 0.216 0-25.20 (r=0.999 6), 0.295 0-12.45 (r=1.000), 0.653 0-65.33 (r=0.999 6),0.393 0-78.32 mg·L-1(r=0.999 8), respectively. The average recoveries were 97.95%, 96.70%, 97.65%, 96.01%, 99.73%, 100.30%. Conclusion: The results indicated that the developed method could be considered to be a simple, rapid and accurate method for the quality control of Alismatis Rhizoma.关键词：Alismatis Rhizoma;triterpenoids;UFLC;quality control
- 3
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To establish a quantitative determination of ginsenosides Rg1, Re, Rb1 and notoginsenoside R1 in Fufang Danshen tablets based on the quantitative analysis of multi-components by single marker (QAMS). Method: RP-HPLC method was used ginsenoside Rb1 as internal reference standard, and 3 relative correction factors (RCF) to ginsenoside Rb1 were calculated. The contents of ginsenosides Rg1, Re, Rb1 and notoginsenoside R1 in Fufang Danshen tablets were determined by both external standard method and QAMS. The differences between the two methods were evaluated. Result: A QAMS method for simultaneous determination of ginsenosides Rg1, Re, Rb1 and notoginsenoside R1 in Fufang Danshen tablets has been established. No significant differences were found in the quantitative results of ginsenosides Rg1, Re, Rb1 and notoginsenoside R1 in Fufang Danshen tablets determined by the two methods. Conclusion: The QAMS method is rapid, accurate, and can save reference substances. QAMS is suitable for quality control of Fufang Danshen tablets.关键词：quantitative analysis of multi-components by single marker;Fufang Danshen Tablets;RP-HPLC
- 3
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: This paper reported the application of near infrared spectroscopy with PLS for rapid quantitative determination of ethanol-extract in Aurantii Fructus Immaturus. Method: Through collecting near-infrared spectra of Aurantii Fructus Immaturus by near-infrared diffuse reflectance spectroscopy technique, with hot-dip method measurements as a reference value, the quantitative model of ethanol-extract was built by partial least squares (PLS), and it was verified by the unknown samples. Result: The correlation coefficients (R2), root mean square error of cross validation (RMSECV), and the root mean-square error of calibration (RMSEC) of the quantitative calibration model of ethanol-extract was 0.984 89, 0.417 89 and 0.153, respectively;the correlation coefficients of predication(r) and the root-mean-square error of predication (RMSEP) was 0.948 4 and 0.188, respectively. Conclusion: The method is simple, quick, accurate and pollution-free, and it can be applied for the rapid determination of ethanol-extract in Aurantii Fructus Immaturus.关键词：near-infrared spectroscopy;partial least square;Aurantii Fructus;ethanol-extract;rapid determination
- 3
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC fingerprint method for quality control of Jiangnian capsule. Method: The HPLC separation was performed on an Agilent C18 (4.6 mm×250 mm, 5 μm), using acetonitrile-0.05%H3PO4 water solution as mobile phase with flow rate of 1.0 mL·min-1. The detection wavelength was 272 nm. Column temperature was set at 35℃. Injection volume was 10 μL.The 'Similarity Evaluation System for Chromatographic Fingerprint of TCM'(2004 A edition) was used to evaluate the similarity of the 10 batches of Jiangnian capsule, and to analyze the correlations of 12 ingredients in Jiangnian capsule. Result: The similarity values of 10 batches Jiangnian capsule were between 0.97-0.99.Taking puerarin as the reference peak, 28 common peaks were found, of which 23 peaks were assigned to herbs, and 4 peaks were identified. Conclusion: The method has good precision, repeatability, stability, and can be used in the comprehensive quality evaluation of Jiangnian capsule.关键词：Jiangnian capsule;fingerprint;similarity;HPLC
- 3
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To observe the differences of composition in volatile oil and anti-tumor effect in vitro within the different cultivated species and different processing products of Curcuma wenyujin. Method: The volatile oil was extracted by steam distillation, identified by GC-MS, then compared the inhibitive effect on cell line MGC-803 in vitro. Result: Although coming from the same Area, the different cultivated species and different processing products of C. wenyujin have obvious different inhibition on the growth of MGC-803 and have significantly when the volatile oil of C. wenyujin for high concentration, breed of fresh products inhibiting growth of MGC-803 was the highest was 20.2%. When the volatile oil of C. wenyujin for low concentration, common varieties of fresh products inhibiting growth of MGC-803 was the highest was 18.4%. Different chemical compositions in volatile oil. The chemical components are also obvious differences. Such as is beta.-phellandrene bicyclo[3.1.1]heptane, 6,6-dimethyl-2-methylene, (1S)-is a characteristic component of traditional fresh product, cyclohexane, 1-ethenyl-1-methyl-2-(1-methylethenyl)-4-(1-methylethylidene )- is the characteristics of chemical composition of common fresh product, bicyclo[3.1.0]hex-2-ene, 4-methyl-1-(1-methylethyl), beta-pinene is the tissue culture of fresh products for characteristic components. Conclusion: Although coming from the same origin, the cultivated species and the processing method of C. wenyujin have great effects on the content and antitumorus activity of the volatile oil. The volatile oil of fresh and processed products breed the strongest inhibitory effect on MGC-803.关键词：Curcuma wenyujin;volatile oil;anti-tumor;GC-MS.
- 3
- |
- 4
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To evaluate the quality of Glycyrrhizae Radix et Rhizoma based on Ggrey incidence degree method. Method: 30 samples of Glycyrrhizae Radix et Rhizoma were collected in Gansu, Inner Mongolia, Xinjiang and other representative habitats. On the basis of traditional appearance identification combined with the market present situation hierarchies of the samples for the determination of extract in samples, the contents of total polysaccharide, glycyrrhizic acid and liquiritin. Grey model was used by determining four main compositions contained in the samples. Result: The result of evaluating the quality of 30 samples was as same as that of medicinal products classification by the model. Samples were divided grade for the first, second, third sort of priorities by the quality of different grades.It was the best licorice of the same grade from different areas in Qingyang, Inner Mongolia, followed by Jinta, Minqin of Gansu province, Hangjinqi of Inner Mongolia, Jiuquan of Gansu province, Xinjiang korla and Zhongning of Ningxia. Licorice samples for the first grade in Shaanxi, Gansu were lower quality than the second licorice in Inner Mongolia. Conclusion: Grey incidence degree method and the model could be used to evaluate the quality of Glycyrrhizae Radix et Rhizoma.关键词：Glycyrrhizae Radix et Rhizoma;quality evaluation;grey incidence degree method
- 3
- |
- 3
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To establish an indirect atomic absorption spectrometry method for determination content of mangifer in Ermu Ningsou Wan. Method: Based on the mangifer can be quantitatively generate brown precipitation with Sr2+ in alkaline environment,the content of mangifer in samples was determined indirectly by atomic absorption spectrometry. The effects of temperature, reaction time, pH value and amount of strontium chloride were studied. Result: The conditions of precipitate reaction were chosen as follow:1.00 mL strontium chloride solutions (1.395 g·L-1), 40℃, pH 9.0, and react 20 minutes. Meanwhile, the linear range was 2.11 to 59.95 mg·L-1 and the detection limit was 0.25 mg·L-1. The recovery rate was 96.74%±1.07%. This method performed good stability, precision and repeatability. Conclusion: The proposed method is easy to control and allows the determination of mangifer in a wide range. The results for the analysis are satisfactory.关键词：flame atomic absorption spectrometry;indirect;mangifer;strontium chloride.
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To identify fastly and accurately in Curcumae Radix of multiple sources by electronic nose. Method: The detective parameters of the samples and metal sensors were selected by detector response values and the characteristic datum were used for statistical analysis through principal component analysis and discrimental factor analysis. Result: The electronic nose detection method of Curcumae Radix were established and the odor identification fingerprint of Curcumae Radix were obtained. Conclusion: The method was used for identifying Curcumae Radix of multiple sources by electronic nose to provide a new idea for the identification of traditional Chinese medicine of multiple sources.关键词：Curcumae Radix;electronic nose;identification
- 3
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To explore the absorption kinetics and absorption mechanisms of cinobufagin in the gastrointestine of rats, and to provide a basis for the development of cinobufagin. Method: The absorption kinetics were obtained by using the in situ perfusion method in rat. The concentration was determinated using HPLC method. Result: After 3 hours perfusion, cinobufagin in different concentrations (11,22,33 mg·L-1) can be better absorpted both in the stomach and small intestine, and the cumulative uptake was 75%. The absorption kinetics were independent of the concentration of the drug. Absorption in the small intestine showed obvious first-order kinetics characteristics, and absorption rate constant of three concentrations was 0.548, 0.547, 0.590 h-1, respectively. Cinobufagin was absorbed in all segments of intestine, and it showed a first-order process with passive diffusion mechanism. The absorption rate constant in the duodenum, jejunum, ileum, and colon were 0.024 6, 0.015 2, 0.016 4, 0.013 5 cm-1·h-1, respectively. Conclusion: Cinobufagin was absorbed in the stomach and all segments of intestine of rats, and its absorption kinetics was a first-order process with passive diffusion mechanism.关键词：cinobufagin;Absorption Kinetics;HPLC;gastrointestinal in situ
- 3
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study the effects of total flavones of Galium verum (FGV) on protecting in human umbilical vein endothelial cells (HUVECs) injured by hydrogen dioxide (H2O2). Method: HUVECs were cultured in vitro. An H2O2injured model was established, FGV at dose of 12.5, 25, 50 mg·L-1was cultured with the cells for 24 h before the model establishment. HUVECs were randomly divided into five groups:normal control group, model group, and FGV group (12.5, 25, 50 mg·L-1).Cell viability was measured by methyl thiazolyl tetrazolium (MTT) assay. The activity of superoxide dismutase (SOD) and the secretion levels of malondialdehyde (MDA) were determined by spectrophotometry. The secretion levels of thrombomodulin (TM) and vascular endothelial growth factor (VEGF) were determined using ELISA. The expression levels of NF-kappa B (NF-κB) mRNA and NF-kappa B inhibitor (IκB) mRNA were measured by RT-PCR. Result: The content of MDA,TM,VEGF and NF-κB mRNA were markedly increased in model group(P<0.01). Compared to model group, FGV group could reduce the proliferation inhibition of HUVECs, depress the content of MDA, improve the activity of SOD, decrease the secretion levels of TM and VEGF, down-regulated the expression of NF-κB mRNA(P<0.05, P<0.01). Conclusion: FGV has a protective effect on the HUVECs impairment induced by H2O2, and the potential mechanism of action may be associated with down-regulating the expression of NF-κB mRNA.关键词：total flavones of Galium verum;human umbilical vein endothelial cells;hydrogen dioxide;antioxidant;NF-kappa B/NF-kappa B inhibitor
- 3
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Periploca forrestii against osteoclast differentiation and determine its active fraction. Method: The three indicators-morphological observation, hematoxylin and eosin(HE)staining, tartrate-resisstant acid phosphatase(TRAP) staining were used to identify osteoclasts which was obtained by vitamin D3(VD3) inducing rabbit bone marrow cells. The active fraction was determined, the inhibitory effects of each polarity of P. forrestii with different doses-low, medium and high dose groups (1, 10,100 mg·L-1) on the TRAP enzyme activity of osteoclasts, TRAP+ cells and bone resorption whirlpool area were measured. Result: In addition to the high-dose of water-saturated n-butanol layer, high-dose group as well as middle-dose of ethyl acetate layer also had significant inhibition on marrow cells differentiating into osteoclasts with dose-dependent manner(P<0.01).The measuring result of the bone resorption ability of osteoclast, showed that ethyl acetate layer inhibited bone resorption whirlpool area dose-dependently (P<0.01), while the high-dose group of n-butanol layer also had apparent effects (P<0.01). Conclusion: Ethyl acetate fraction is the most active part of P. forrestii to suppress differentiation of bone marrow cells into osteoclast and bone resorption capacity.关键词：Periploca forrestii;osteoclasts;active fraction;bone resorption;ethyl acetate layer
- 3
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro effects of water extract components of Oxalis corniculata on cell proliferation and differentiation in human osteoblast-like SaOS-2 cells. Method: SaOS-2 cells were treated with different concentrations (10, 100, 200 mg·L-1) of water extract components, and then cell proliferation and alkaline phosphatase (ALP) activity were measured by MTT assay and p-nitrophenyl phosphate(PNPP) method respectively, secretions of Ca2+, osteocalcin (OTC) were detected by enzyme-linked immunosorhant assay(ELISA), and osteoblast mineralization nodules were measured by alizarin red staining method. Result: Compared with control group, water extract components of O. corniculata significantly promote proliferation of SaOS-2 cells (P<0.01), and it can also increase ALP activity in time-dependent manner (P<0.05), especially at the concentration of 100 mg·L-1(P<0.01). Moreover, it can increase secretion of Ca2+(P<0.05) and OTC(P<0.01),and significantly promote formation of osteoblast mineralization nodules(P<0.05)at high and medium concentration. Conclusion: Water extract components of O. corniculata can promote proliferation, differentiation and mineralization of osteoblast. These results suggest that O. corniculata can promote bone formation.关键词：Oxalis corniculata;Osteoblast;proliferation;differentiation;mineralization
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the spectrum-effect relationship of leaf extracts from Mangifera indica (LEMI) based on grey relational analysis. Method: High performance liquid chromatography (HPLC) was applied to establish the fingerprint chromatography of the leaf extracts extracted with different polar solvent. The anti-inflammatory effects of the extracts were observed via mice auricular swelling model induced by dimethylbenzene. Kunming mice were randomly divided into model group, dexamethasone positive group and LEMI groups. Model group was administered orally physiological saline;other groups were given corresponding drug for 5 days. Then ear swelling was determined. Then the grey relational analysis was used to investigate its spectrum-effect relationship. Result: There were significantly differences in the HPLC fingerprints and anti-inflammatory activities obtained from varied extracts. Compared with the model group, dexamethasone group had significantly inhibited the ear edema in mice (P<0.05). Chloroform extract and ethyl acetate LEMI from Red-ivory and Tainong-1 showed no significant anti-inflammatory effect, but other solvent LEMI had significant anti-inflammatory effect (P<0.05). 14 common peaks were detected in HPLC chromatography, which were used for the identification of herb products. It was found that the main anti-inflammatory effect was mostly contributed to X1, X3and mangiferin after grey relational analysis. Conclusion: The material basis of anti-inflammatory effect of LEMI were mangiferin, X1 and X3.Grey relational analysis was an effective and useful method to evaluate spectrum-effect relationship of Chinese herbs.关键词：Mangifera indica;anti-inflammatory effect;spectrum-effect relationship;grey relational analysis;mangiferin;fingerprint chromatography
- 3
- |
- 3
- |
- 15
发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Shugan Jieyu capsule(SGJY) on learning and memory ability in model mice with Alzheimer's disease (AD). Method: Seventy-two Kunming (KM) mice were randomly divided into six groups such as normal group, model group, huperzine A(Hup A) group(0.07 mg·kg-1)and SGJY groups (480, 240, 120 mg·kg-1). The mice were gavaged once a day for 14 days, then induced by scopolamine hydrobromide (ip, 3 mg·kg-1) except normal group. The effect of SGJY was studied via Morris water maze test and the levels of malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in plasma and brain tissue were detected. Randomly selected 10 non-APPswe transgenic mice were as normal group, other 50 APPswe transgenic mice with high expression of presenilin (PS) and amyloid precusor protein (APP) were divided into five groups as above, the mice were gavaged once a day for 30 days, then the effect of SGJY on learning and memory ability was evaluated by step-down test and water maze test. Result: Compared with normal groups, the levels of latencies and total distances before escaping onto the security platform increased significantly(P<0.05, P<0.01). Compared with model groups, SGJY groups in various dosage (480, 240, 120 mg·kg-1) could shorten the latency period to escape from electric shock. In Morris water maze test, three groups of SGJY could shorten latencies and total distances before escaping onto the security platform at different levels. The effect was the most obvious in the group of high and middle dose SGJY(P<0.01), which showed dose-effect relationship. Meanwhile, we also found that the SGJY of three different doses could significantly decrease the levels of MDA and increase that of T-AOC in brain tissue (P<0.01). Conclusion: SGJY has marked effect of improving the learning and memory abilities of AD model mice.关键词：Shugan Jieyu capsule;Alzheimer's disease;Scopolamine hydrobromide;APPswe transgenic mice
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of rutaecarpine (Rut) on the expression of Toll like receptor 2/4 (TLR2/4) and nuclear factor kappa B (NF-κB) in permanent focal cerebral ischemia rats and to explore the mechanism involved. Method: The rat permanent middle cerebral artery occlusion (pMCAO) model was reproduced according to the revised Koizumi method. Three days after the surgery, rats were divided into model group, sham group, and rutaecarpine(Rut) group. Rut was injected intraperitoneally at the doses of 10, 5, 1 mg·kg-1 for seven days. The cerebral infarction area and water content of brain tissue were determined. Protein expression of TLR2/4 and NF-κB in brain tissue was detected by Western blot and immunohistochemical methods. Result: Compared with the sham group, the infarct volume and brain water content were significantly increased after pMCAO;the protein expression of TLR2, TLR4, and NF-κB were increased significantly with western bloting and immunochemical methods (P<0.01), andincreased TNF-alpha and IL-1 beta levels in serum as well (P<0.01). Rutaecarpine could significantly reduce infarct volume and brain water content (P<0.05),the expression of TLR2/4 and NF-κB in rats was significantly attenuated(P<0.05), and TNF-alpha and IL-1beta content was alleviated(P<0.05). Conclusion: Rut can ameliorate the rats focal cerebral ischemic injury, and the mechanism may be related with the regulation of TLR2/4-NF-κB signaling.
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Ailuo Kechuanning on levels of soluble intercellular adhesion molecular-1(sICAM-1),tumor necrosis factor alpha(TNF-α),interleukin-8(IL-8),interleukin-17(IL-17) and C-reactive protein(CRP) in serum,bronchial alveolar lavage fluid (BALF) and lung homogenates of rats with chronic obstructive pulmonary disease (COPD). Method: The method of smoke add drops of bacteria preparation was used to rat model COPD with stabilization and acute aggravatin.the rats were randomly divided into normal control group,model group,Ailuo Kechuanning group (7.75,15.52,31.04 g·kg-1·d-1),ELISA was used to determine the levels of sICAM-1,TNF-α, IL-8,IL-17 and CRP in serum, BALF and lung homogenates. Result: ①Stable period:the total number of neutrophils and inflammatory cells were significantly increased (P<0.01) in BALF.The levels of sICAM-1,TNF-α,IL-8,IL-17 and CRP in serum, BALF and lung homogenates of the stable period model group were significantly higher than those in normal control group. While the levels of sICAM-1,TNF-α,IL-8,IL-17 and CRP were decreased significantly in serum, BALF and lung homogenates of the rats treated with Ailuo Kechuanning(15.52 g·kg-1·d-1)than those in stable period model group.②Acute exacerbation of COPD:the obvervational index changes direction in acute exacerbation period tend to be consistent with stabilization period. Conclusion: The mechanism of Ailuo Kechuanning anti-inflammatory may reduce the number of neutrophils and macrophages, and decrease the levels of sICAM-1,TNF-α, IL-8,IL-17 and CRP.关键词：chronic obstructive pulmonary disease;bronchial alveolar lavage fluid;tumor necrosis factor alpha;interleukin-8
- 3
- |
- 3
- |
- 7
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Yifei Tongluo formula on muscular arteries medial smooth muscle cell proliferation of hypoxia-induced pulmonary vascular remodeling in rat. Method: Sixty Wistar rats were divided into 5 groups:blank group,model group,experimental group(high-does, middle-does, low-does),12 rats in each group. The trachea injection of 0.2 mL lipopolysaccharide (1 g·L-1)combined smoke method for four weeks was adopted to establish the model of chronic obstructive pulmonary disease(COPD),and superposition of low oxygen(18%) two weeks establish pulmonary vascular remodeling(PVR) models in rats. The blank and model groups were perfused with saline, experimental group was perfused with Yifei Tongluo formula (30, 20, 10 g·kg-1). Samples were collected in 4,5,6 weeks respectively. The middle membrane thickness, vascular diameter and middle membrane area, vascular area were measured, then calculated the ratio of relative middle membrane thickness and area. Result: The ratio of relative middle membrane thickness and area in each point-in-time between the groups showed t as follows:model group> low-does group> middle-does> high-does> blank group, with significant difference. Conclusion: Yifei Tongluo formula can inhibit the muscular arteries medial smooth muscle cell proliferation of hypoxia-induced pulmonary vascular remodeling in rats.关键词：pulmonary vascular remodeling;vascular smooth muscle cell proliferation;Yifei Tongluo formula
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Danzhi Jiangtang capsules (DJC) on kidney podocyte platelet in type 2 diabetes rat model. Method: Diabetes rat model were established using a high calorie diet and streptozotocin induction (intraperitoneal injection, 35 mg·kg-1). Fifty-five rats were randomly divided into five groups;normal group, model group, high-and low-dose DJC group (1.08, 0.54 g·kg-1), pioglitazone group (10 mg·kg-1). The rats received intragastric administration of corresponding medicines for 8 weeks. The proportion of kidney, urine albumin and serum creatinine were measured, and the podocyte ultrastructure was observed by electron microscope. Result: Glomerular basement membrane was thickened, foot process was fused or even disappeared, foot processes hiatus were reduced, the electron dense deposited in the basement membrane and mitochondria in the cytoplasm of podocytes were significantly reduced, rupture of mitochondrial cristae, vacuolar degeneration and lipid droplets were found in model group. Injury of podocytes in high-and low-dose Danzhi groups was significantly less than that in the model group. Meanwhile, basement membrane thickening was not obvious, foot process was broadened even fused. The result was better in the high-dose DJC group than that in the pioglitazone group. Renal pathology report showed that model group had unclear capillary loops, increased glomerular volume and number, widened mesangial area, increased glomerular cyst cavity and vacuolar degeneration, vascular proliferation in renal interstitial and apparent inflammatory cell infiltration were found. It showed that the above changes had good improvement in high and low-dose DJC groups, and high-dose DJC group obtained better results. Morever, the level of urinary albumin and the proportion of kidneyweight were decreased in the all DJC groups (P<0.01), while serum creatinine has no change as compared with model group. Conclusion: DJC may have kidney protection effect which is contributed by reducing the injury of podocytes and the level of urine micro albumin in diabetic rats.关键词：Yiqi Yangyin Huoxue method;Danzhi Jiangtang capsules;diabetic rat model;kidney podocyte
- 3
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study the influence of Gegen Tongmaiyin on vascular smooth muscle cells proliferation and immunoglobulin heavy chain binding protein (BIP) expression of endoplasmic endoplasmicreticulum stress gene in homocysteine-induced rabbits, and to explore its preventive mechanism on atherosclerosis. Method: Thirty rabbits were randomly divided into five groups:normal group, high-fat diet model group, rosuvastatin group (1 mg·kg-1·d-1), low-and high-dose Gegen Tongmaiyin group (5, 10 g·kg-1·d-1). Each group was given the corresponding diet and drug. After nine weeks, the serum levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) were detected by enzymatic assays. Pathological change and BIP expression of vascular were detected. Result: Compared to high-fat diet model group, plasma TC, TG, LDL in rosuvastatin group and Gegen Tongmaiyin group were decreased, while plasma HDL was increased with significant difference (P<0.05, P<0.01). Compared to high-fat diet model group, BIP expression was down-regulated in rosuvastatin group, low-and high-dose Gegen Tongmaiyin group with significant difference (P<0.05, P<0.01). Pathological result indicated that Gegen Tongmaiyin could inhibited the development of atherosclerosis. Conclusion: Gegen Tongmaiyin could prevente atherosclerosis, which is related to the regulation of BIP expression.关键词：Gegen Tongmaiyin;atherosclerosis;immunoglobulin heavy hains binding protein
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Shenlin Baishu San on the dysbacteriosis induced by antibiotics in mice. Method: The healthy SPF BALB/c mice were divided into the normal group, the model control group, the Peifeikang group (0.1 g·kg-1) and the low-, medium-, high-dose Shenlin Baishu San groups (0.1, 0.2, 0.4 g·kg-1). Dysbacteriosis models were induced by antibiotics ceftriaxone sodium solution for 5 days. After 7-day treatment, changes of intestinal flora with sterile take feces were observed using plate dilution method (each intestinal bacteria number was trained). Serum immunoglobulin G (IgG), endotoxin, vasoactive intestinal peptide (VIP) and substance P (SP) content were measured using enzyme linked immunosorbent assay. Result: The number of probiotics in the model group was signifigantly decreased as compared with that in the normal group (P<0.01). Serum levels of endotoxin and SP were significantly higher. serum IgG and VIP were significantly lower in model group than those in control group (P<0.01). The bacteria number, endotoxin, VIP and SP were decreased, serum IgG was increased in all dosages of Shenlin Baishu San groups, there was statistically significant difference as compared with model group (P<0.05, P<0.01). Conclusion: Shenlin Baishu San could regulate the balance of intestinal flora effectively.关键词：dysbacteriosis;antibiotics;Shenlin Baishu San
- 9
- |
- 2
- |
- 32
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects and the mechanisms of synephrine (Syn) on the contraction of isolated guinea-pig tracheal smooth muscle. Method: The guinea-pig tracheal smooth muscle was prepared by isolated tracheal thermostatic perfusion experimental method and isolated in the K-H solution. The tension of Syn on the isolated tracheal smooth muscle and the effect of Syn on tracheal smooth muscle contraction incubation by four antispasmodic agents including acetylcholine (Ach), histamine (His), CaCl2 and β-receptor blocking agent (propranolol) were measured by BL-420S biological function experiment systems. Result: Syn produced a dose-dependent relaxant effect on the isolated guinea-pig tracheal smooth muscle. The value of PD2 was 2.22±0.14.Syn (0.021 2, 0.042 4 mmol·L-1) could reduce the maximum of guinea-pig tracheal smooth muscle contraction induced by Ach and His, and Syn at 0.042 4 mmol·L-1 had significant effect (P<0.05). Syn also could reduce the value of guinea-pig tracheal smooth muscle contraction induced by CaCl2 with no significant differences. Three dosages of Syn (0.010 6,0.021 2, 0.042 4 mmol·L-1) could inhibit the release of internal calcium of tracheal smooth muscle cell induced by Ach significantly in a dose-dependent manner (P<0.05, P<0.01).The propranolol and Syn had no significant reducing effect on tracheal tension. Conclusion: Syn could relax tracheal smooth muscle by acting on M-receptor and histamine receptor, excit β-receptor and inhibiting the release of internal calcium.关键词：synephrine;tracheal smooth muscle;relaxation
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of Liujunzi Tang extract on the proliferation of human esophageal carcinoma cell. Method: Esophageal carcinom Eca109 cells were grown in 96-well plates and treated with different concentrations of Liujunzi Tang extract (10, 20, 40, 60, 80, 100 mg·L-1). There were 8 wells for each concentrations of Liujunzi Tang extract groups and for the normal group. Inhibitive effect of Liujunzi Tang extract on proliferation was detected by MTT assay, cell apoptosis was detected by flow cytometer and cellular morphology was observated by laser scanning confocal microscopy. Result: Liujunzi Tang extract produced a dose-dependent inhibition on the proliferation of Eca109 cells within the concentration of 10-100 mg·L-1 (P<0.05). Compared to the normal group, Liujunzi Tang extract could significantly increase the apoptosis rate of Eca109 cells after being cultered for 48 h at the concentration of 21.06, 49.65, 67.47 mg·L-1 (P<0.05), and the numbers of the apoptotic cells in the early or late stages were increased obviously. Conclusion: Liujunzi Tang extract could significantly inhibit the proliferation of Eca109 cells by inducing cell apoptosis. Its exact mechanism will be further investigated.关键词：Liujunzi Tang extract;esophageal carcinom;Eca109 cell;apoptosis;laser scanning confocal microscopy;flow cytometer
- 3
- |
- 3
- |
- 8
发布时间：2024-01-04 - 摘要：Objective: To observe the antitumor effects of different polarity extracts from Kaki Folium on hepatoma H22 and sarcoma S180 bearing mice. Method: The the Kaki Folium were successively extracted with ethanol, chloroform, ethyl acetate and n-butanol, the ethanol extraction were extracted by water, and the different polarity extracts were obtained. Experimental H22 ascites tumor mice model, H22 and S180 solid tumor mice models were established, respectively. The experimental mice were randomly divided into a series of groups:blank control group, model group, cyclophosphamide group, low-, middle-and high-dosage groups of the various different polarity extracts. The mice were intragastrically administrated the corresponding drugs for 10 days. The abdominal circumference, inhibition rate of ascites and liver tissue physiological form were observed in H22 ascites tumor mice. The food intake, body weight, tumor inhibition rate, life prolongation rate, values of thymus coefficient and spleen coefficient were recorded. Result: Compared with the model group, the volume of ascites and ameliorate liver cell swelling, karyolysis on H22 ascites tumor mice were significantly reduced in ethyl acetate extract, water extract and n-butanol extract groups, while no significant effects on food intake and body weight in H22, S180solid tumor mice were found. The tumor inhibition rate, life prolongation rate, values of thymus coefficient and spleen coefficient were significantly increased (P<0.01 or P<0.05) after treated with different polarity extracts and cyclophosphamide groups. Conclusion: The ethyl acetate extract, water extract and n-butanol from Kaki Folium possess have certain antitumor effects on hepatoma H22 and sarcoma S180 bearing mice, which may be the effective fractions of antitumor effects from persimmon leaves. The ethyl acetate-extracted fraction has better effects on hepatoma H22 bearing mice (included the solid and ascites tumor) than sarcoma S180 bearing mice.关键词：Kaki Folium;polarity parts;hepatoma H22;sarcoma S180;anti-tumor activity
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To compare effects of brucine and brucine-hydroxypropyl-β-cyclodextrin(bru-HP-β-CD) inclusion complex on adjuvant-induced arthritis rats,in order to provide a reference for development of this preparation. Method: After being modeled with Freund's complete adjuvant,effects of brucine and bru-HP-β-CD inclusion complex on pharmacological indexes of adjuvant arthritis rats were detected,including primary and secondary voix pedis swelling,pain threshold,weight and so on. Result: Compared with the model group,brucine group and bru-HP-β-CD inclusion complex group could significantly increase pain threshold and reduce voix pedis swelling.Analgesic effect was the best after proinflammatory for 0.5 h by brucine,its anti-inflammatory effect for primary disease after 48 h was the most significant;however,analgesic effect was optimum after proinflammatory for 1.0 h by bru-HP-β-CD inclusion complex,its anti-inflammatory effects were better than those of brucine group for primary disease after 72 h and secondary disease after 24 d. Conclusion: Anti-inflammatory effect of brucine is rapid action,but effect of bru-HP-β-CD can maintain for a long time and is better than brucine.Brucine can effectively relieve joint pain of rats with adjuvant-induced arthritis after being included by HP-β-CD,and has sustained and promoting effect.关键词：brucine;hydroxypropyl-β-cyclodextrin;inclusion complex;adjuvant-induced arthritis;swelling
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To discuss the curative efficacy of Shenqi Fuzheng injection united meglumine adenosine cyclphosphate in treating chronic heart failure (CHF) and to investigate its influence on troponin T(cTnT), insulin-like growth factor 1(IGF-1) and homocysteine (HCY). Method: Eighty-six patients with CHF were randomly divided into control group (43 cases) and observation group (43 cases). Patients in both groups received conventional non-drug and drug therapies. Drug therapy included 25-50 mg hydrochlorothiazide tablets, 12.5 mg captopril tablets, 50-100 mg metoprolol tartrate tablets twice daily, and 0.25 mg digoxin tablets once daily when needed. Patients in control group received intravenous drip of 120 mL meglumine adenosine cyclphosphate injection once daily. Based on the treatment of control group, patients in observation group added intravenous drip of 250 mL Senqi Fuzheng injection and 120 mg mixture meglumine adenosine cyclphosphate once daily. Patients in two groups received 2-week treatment. Cardiac function was tested by adopt echocardiogram before and after treatment. Levels of N terminal pro-brain natriuretic peptide (NT-proBNP), cTnT, IGF-1 and HCY were detected before and after treatment. Result: By Ridit analysis, the curative effect of observation group was superior to that of control group (P<0.05). The levels of left ventricular ejection fraction(LVEF), output per mimute(CO) and stroke volume(SV) of observation group were higher than those of control group (P<0.01). Levels of NT-proBNP, cTnT and HCY in observation group were less than those in control group (P<0.01), while the IGF-1 of observation group was superior to that of control group (P<0.01). Conclusion: Using Senqi Fuzheng injection combined with meglumine adenosine cyclphosphate could enhance the levels of LVEF, CO, SV in CHF patients and improve heart function significantly. Its mechanism may related to improving IGF-1 level and reduce the NT-proBNP, cTnT and HCY levels.关键词：chronic heart failure;Shenqi Fuzheng injection;meglumine adenosine cyclphosphate;troponin T;insulin-like growth factor 1;homocysteine
- 3
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe the curative efficacy of conservative treatment of Qianlie Longbitong tablets on benign prostatic hyperplasia (BPH) and to discuss its influence on blood rheology. Method: Ninety patients were randomly divided into control group (45 cases) and observation group (45 cases) by random number table. Patients in control group received orally 0.2 mg tamsulosin hydrochloride sustained release capsules once every night and 5 mg finasteride tablets once daily. Patients in observation group received orally 4 Qianlie Longbitong tablets thrice daily. Patients in two groups received 12 weeks of treatment. International prostate symptom score (I-PSS), kidney (gas) deficiency and blood stasis score were evaluated before and after treatment. Size of the prostate, residual urine volume (PVR), maximum urinary flow rate (Qmax), average urinary flow rate (Qave) were detected via type-B ultrasonic. The influence of urination on quality of life was evaluated. The blood rheology was tested before and after treatment. Result: Through Ridit analysis, the total efficacy of disease, kidney (gas) deficiency and blood stasis curative effect in observation group were superior to those in control group (P<0.05). After treatment, scores of I-PSS, QOL, kidney (gas) deficiency and blood stasis in observation group were less than those in control group (P<0.01). After treatment, prostate volume in two group was smaller as compared with that before (P<0.01 or P<0.05). PVR of observation group was lower than that of control group (P<0.01). Qmax and Qave of observation group were higher than those of control group (P<0.01). Besides, the improvement of blood rheology indexes as whole blood viscosity (low cut and high cut), plasma viscosity and whole blood reductive viscosity (low cut and high cut) in observation group were superior to those in control group (P<0.01). Conclusion: Qianlie Longbitong tablets could shrink prostate volume with BPH (kidney deficiency and blood stasis) patients, relieve the symptoms of urinary tract and improve patient quality of life. It has certain effects on the total efficacy of disease and improvement in traditional Chinese medical syndrome.关键词：benign prostatic hyperplasia;Qianlie Longbitong tablets;blood rheology
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To discuss the influence of Shenhe Zhigan decoction on sugar, lipid and uric acid metabolism in treating patients with nonalcoholic fatty liver disease (NAFLD) (liver stagnation and spleen deficiency combined with phlegm and blood stasis) and to discuss its resistance to oxidative stress. Method: One hundred and sixteen patients with NAFLD were randomly divided into control group (58 cases) and observation group (58 cases) by random number table. Patients in both groups received orally 10 mg vitamin E thrice daily, and 10 mg simvastatin tablets once daily. Patients in control group added orally 2 Xuezhikang tablets twice daily, and patients in observation group added 1 does Shenhe Zhigan decoction once daily for 12 weeks. Liver and spleen CT ratio were evaluated before and after treatment. Levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL-C), low density lipoprotein (LDL-C) and blood uric acid (SUV) were tested before and after treatment. Fasting blood glucose (FBG), fasting insulin (FINS) were detected and indexe of insulin resistance (HOMA IR) was calculated. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were tested before and after therapy. Result: ① Fatty liver curative effects the improvement of liver/spleen CT ratio in observation group was superior to that in control group (P<0.05) by the chi-square test order data. Besides, curative effect of fatty liver in observation group was better than that in control group (P<0.05). ②Influence of sugar, lipid and uric acid metabolism TG and TC levels in observation group were lower than those in control group (P<0.01). Abnormal cases of TG and TC in observation group were less than those in control group (P<0.05). FINS and IR levels in observation group were lower than thoses in control group (P<0.01). Level of SUV in observation group was inferior to that in control group (P<0.01). ③Resistance to oxidative stress Level of SOD in observation group was higher than that in control group, while the MDA level in observation group was less than that in control group (P<0.01). Conclusion: Shenhe Zhigan decoction could regulate the metabolism of sugar, lipid and uric acid in NAFLD patients (liver stagnation and spleen deficiency combined with phlegm and blood stasis), and improve the IR index. It has a certain resistance to oxidative stress and has a good effect in treating NAFLD as compared with Xuezhikang.关键词：nonalcoholic fatty liver disease;Shenhe Zhigan decoction;blood uric acid;insulin resistance;oxidative stress
- 3
- |
- 3
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy and the possible mechanisms of Chaixie Danshen decoction in treating unstable angina pectoris (UAP) patients of Qi stagnation and blood stasis type. Method: Eighty-two patients with UAP were randomly divided into observation group (42 cases) and control group (40 cases). Patients in both groups received conventional western medical treatment, and patients in observation group added Chaixie Danshen decoction. All patients received 6 weeks of treatment. The clinical efficacy and the change of Chinese medical syndrome between the two groups were compared before and after treatment. The serum levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor alpha (TNF-a), interleukin 6 (IL-6) and matrix metalloproteinase-9 (MMP-9) were observed before and after treatment. Result: The total curative rate of the observation group (90.48%) was better than that (70.00%) of the control group (P<0.05). The total scores of Chinese medical syndrome and the single score for chest pain, chest tightness, breathe hard, palpitations after treatment were lower than those before treatment in two groups;score changes in observation group were superior to that in control group (P<0.01). The effective rate of Chinese medical syndrome in the observation group (92.86%) was better than that (67.50%) in the control group (P<0.05). Compared with before, the levels of hs-CRP, TNF-α, IL-6 and MMP-9 were significantly decreased in two groups (P<0.05 or P<0.01), and the observation group obtained better results (P<0.05). Conclusion: Chaixie Danshen decoction could improve symptoms in UAP patients of Qi stagnation and blood stasis type and reduce the levels of hs-CRP, TNF-α, IL-6 and MMP-9.These findings indicate that Chaixie Danshen decoction may have anti-inflammation and anti-atherosclerosis by regulating the levels of inflammatory cytokines of patients with UAP.关键词：Chaixie Danshen decoction;high-sensitivity c-reactive protein;tumor necrosis factor alpha;interleukin 6;matrix metalloproteinase-9;unstable angina pectoris;Qi stagnation and blood stasis type
- 3
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Danggui Shaoyao San on alcoholic cirrhosis with portal hypertension ascites and to discuss its mechanism. Method: A randomized controlled trial was conducted. Seventy patients were assigned to western medical treatment (control group) and western medical treatment plus Danggui Shaoyao San (treatment group) for 1 month. The changes of body weight, abdominal perimeter and amount of urine were observed before and after treatment. The data related to portal hypertension was examined using color doppler ultrasound. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) levels were measured via automatic biochemical analysis, plasma prothrombin time activity (PTA) level was measured via automatic coagulation analysis, endothelin-1 (ET-1) was measured via ELISA, svascular endothelial growth factor (VEGF) was measured via radioimmunoassay. Result: There was no significance difference for the baseline between two groups. Compared with control group, body weight, abdominal perimeter, amount of urine, data related to portal hypertension, liver function (TBIL, ALB) and coagulation function (PTA) were remarkably improved in treatment group (P<0.05). The levels of serum ET-1 and VEGF were significantly decreased in treatment group as compared with control group (P<0.05). However, there was no significance difference in serum ALT levels between two groups. Conclusion: Danggui Shaoyao San could significantly improve portal hypertension ascites, liver function and coagulation function in patients with alcoholic cirrhosis, and its mechanism may be related to declining the activity of ET-1 and VEGF.关键词：cirrhosis;ascites;Danggui Shaoyao San;mechanism
- 3
- |
- 3
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To evaluate the therapeutic effect of Xiaoke Jiangtang Fang combined with pioglitazone hydrochloride and metformin hydrochloride tablets on patients with type 2 diabetes. Method: Ninty-eight patients with type 2 diabetes were randomly divided into observation group (48 cases, Xiaoke Jiangtang Fang plus pioglitazone hydrochloride and metformin hydrochloride tablets) and control group (48 cases, pioglitazone hydrochloride and metformin hydrochloride tablets). The body mass index (BMI), fasting blood glucose (FBG), 2 hour postprandial blood glucose (2 hPG), hemoglobin A1c (HbA1c), low density lipoprotein (LDL), high density lipoprotein (HDL), triglyceride (TG), high/midst/low shear rate of blood viscosity, plasma viscosity, fibrinogen, the clinical symptom, and Chinese medical symptom were calculated and compared. Result: After 3 courses of treatment, the effective rate of observer group (42/48, 87.5%) was significantly higher than control group (32/48, 66.7%) (P<0.05). After treatment, the BMI, FBG, 2 hPG, HbA1c,TG, fibrinogen, high/midst/low shear rate of blood viscosity, and plasma viscosity in two groups were significant decreaseded (P<0.05). However, the FBG [(6.42±1.14) VS (6.89±1.02) mmol·L-1], 2 hPG [(9.12±1.53) VS (9.79±1.68)mmol·L-1], HbA1c[(6.22±1.51)% VS (6.89±1.73)%], TG[(4.09±1.12) VS (4.93±1.31)mmol·L-1], fibrinogen[(2.26±0.94) VS(3.26±1.05) g·L-1], high (4.01±1.17) VS (4.73±1.51)mPa·s/midst (5.47±2.08) VS (6.84±2.30) mPa·s/low (8.29±3.07) VS (10.83±3.26) mPa·s shear rate of blood viscosity, and plasma viscosity (1.24±0.37) VS (1.82±0.62) mPa·s in observation group were significant lower than those of control group (P<0.05). The score of traditional Chinese medicine symptom in observer group was significant lower than that of control group after treatment (P<0.01). Conclusion: Xiaoke Jiangtang Fang combined with pioglitazone hydrochloride and metformin hydrochloride tablets have an obviously curative effect on patients with type 2 diabetes.关键词：type 2 diabetes;Xiaoke Jiangtang Fang;pioglitazone hydrochloride and metformin hydrochloride tablets
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the efficacy and safety of Shenqi Fuzheng injection(SQFZI) combined with radiotherapy in treating non-small cell lung cancer (NSCLC). Method: Systematic literature searches were performed in PubMed, CBM, CNKI, VIP and WanFang Data from database inception to May 2014.Randomized controlled trials(RCTs) of SQFZI combined with radiotherapy (versus radiotherapy alone) in any language were included. No language limitation was applied.Two reviewers separately assessed the quality of each included study and extracted the data. Meta-analysis was carried out according to the method provided by the Cochrane Collaboration with RevMan5.2 software. Result: A total of 7 RCTs were included. The Meta-analysis showed that the combination of SQFZI and radiotherapy couldimprove the curative effect [OR=2.74, 95%CI(1.85,4.05), P<0.000 01], improve the quality of life [OR=4.85, 95%CI (2.65, 8.89), P<0.000 01], improve clinical symptom[OR=4.23, 95%CI(2.33, 7.69), P<0.000 01], reduce myelosuppression [OR=0.28, 95%CI(0.15, 0.51), P<0.000 1], radiation pneumonitis [OR=0.34, 95%CI(0.20, 0.58), P<0.000 1] and radiation esophagitis[OR=0.40, 95%CI (0.27, 0.61), P<0.000 1] as compared with radiotherapy alone. Conclusion: The current results indicates that SQFZI can improve the curative effect on NSCLC patients, improve the quality of life, improve clinical symptom and reduce the adverse effect of radiotherapy. These findings require further confirmation with rigorously controlled trials.关键词：Shenqi Fuzheng injection;radiotherapy;non-small cell lung cancer;Meta-analysis
- 3
- |
- 3
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To explore the rules among external therapy and food therapy of traditional Chinese medicine (TCM) in treating diabetes by applying text mining technology. Method: The relative literatures were collected from Sinomed database (CBM), and an Access database was established. The data was processed by SQL using frequency statistical method based on sensitive keywords hierarchical algorithm, and noise was manually removed according to the medical professional knowledge. The rules among external therapy and food therapy of TCM were deeply analysed and comprehensively displayed by the frequency of one-dimensional and two-dimensional network diagram. Result: The results showed that the most common external therapies of diabetes were acupuncture (frequency 2 079),phototherapy (frequency 1 237), external application with TCM (frequency 348), acupiont injection (frequency 327) and massage (frequency 207) in turn. The most common food therapies were followed by panax notoginseng (frequency 446), Chinese yam (frequency 262), lemon (frequency 234) and wolfberry (frequency 143). Conclusion: Text mining, together with artificial reading for anti-noising, is an useful approach in exploring the clinical rules of TCM in treating diabetes. However, its exact mechanism and certain indication in treating diabetes is worthy of further study.关键词：text mining;diabetes;external therapy of traditional Chinese medicine;food therapy of traditional Chinese medicine
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To analyze the composing principles of the prescriptions for amnesia collected in . Method: The prescriptions used for amnesia in were collected and the composing principles were analized by traditional Chinese medicine inheritance support system using the entered data. Result: One hundred and thirty-four prescriptions and the frequency of each herb were studied.Ten commonly-used herbs and 4 core herbs are identified. Conclusion: The main therapy for amnesia should be the herbs of invigorating the heart and spleen, waking up the patient from unconsciousness by dissipating phlegm, tranquilizing and improving intelligence, and some herbs with the efficacy of clearing heat, activating blood, regulating Qi and nourishing Yin could be added according to the different accompanied symptoms. Therefore, it could achieve the results of treating both cause and symptoms.关键词：prescription;amnesia;Traditional Chinese Medicine Inheritance Support System;composition principle
- 3
- |
- 3
- |
- 3
发布时间：2024-01-04 -
Improved Algorithm About Pre-evaluation Method of Traditional Chinese Medicine Clinical Prescription
摘要：Objective: We want to develop a Pre-evaluation method of traditional Chinese medicine(TCM) clinical prescription based on the improved algorithm,which can carry out the intelligent recognition and analysis of TCM clinical prescription. Method: Using mathematical methods,improved the method based on the calculation of Chinese medicine weighted,and the method is verified and analyzed by using two commonly used clinical prescription. Result: The results of classical TCM prescription of Suanzaoren Tang is sedative drugs found to account for 0.46 of the total party weights, so it can be inferred from the results for ‘the party is mainly composed of sedative drugs constitute’;analysis of the traditional Chinese medicine prescription Guilu Erxian Jiao is found that the number of Bhd drug ingredients for 1, accounted for the whole Party weight 0.56, Yin tonic medicine number 2, a total of 0.38 weights, so it can be inferred from the results for ‘the party is mainly composed of Yin tonic, with tonifying-Yang drug assisted’. Conclusion: This method can identify and analyze the accuracy of the clinical medical prescription of TCM.关键词：pre-evaluation of traditional Chinese medicine clinical prescription;algorithm;TCM prescriptions;intelligent analysis- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：By reviewing the literatures in recent years, the latest progress on chemical components, pharmacology, toxicity studies and metabonomics of Toosendan Fructus have been arranged and analysed. The previous researchs have shown that toosendanin, isolated from Toosendan Fructus, have certain toxicity. The summary not only illustrated the compatibility attenuated mechanism of Toosendan Fructus based on absorption and metabolism, but also revealed its rational and scientific connotations of compatibility. As a traditional Chinese herb Toosendan Fructus has been extensively applied in clinics. However, the current researchs about pharmacological effect of Toosendan Fructus are focused on its neem alkyl-type triterpenoids and limonoids compounds. So far, there is limited systemic research on therapeutic material basis of Toosendan Fructus. In order to ensure its safe and effective application in clinics, the therapeutic basis in vivo should be seeked by comprehensive analysis using serum chemistry combined PK/PD studies.关键词：Toosendan Fructus;chemical constituents;pharmacology;toxicity;compatibility attenuated;metabonomics
- 3
- |
- 2
- |
- 12
发布时间：2024-01-04 - 摘要：Based on the related literature both domestic and abroad, this paper summarized the chemical constituents and pharmacological activities of Amorpha fruticosa in order to provide the reference for the further study of A. fruticosa. We found that A. fruticosa contained a variety of flavonoids, polyhydrostilbenes and volatile oils etc., which showed various activites including anti-tumor, protecting liver, insecticidal activity, antimicrobial activity, and so on. Herein, we had made some progress on its chemical constituents and pharmacological actions. However, its effective constituents and mechanism of actions have been lack of research. The futher research will be helpful to the development of new medcines and the utilization of plant resources sufficiently.关键词：Amorpha fruticosa;chemical composition;pharmacological effectiveness
- 3
- |
- 4
- |
- 2
发布时间：2024-01-04 - 摘要：As a derivant of chitosan,carboxymethyl chitosan has good performances,such as water solubility,antibacterial activity,adsorbability and so on,its antioxidation,anti-tumor,hemostatic effect are well.Carboxymethyl chitosan calcium has a certain antioxidant capacity and liver protection for acute liver injury mice induced by CCl4.Based on these above investigation,application prospect of carboxymethyl chitosan in medicine,food,cosmetics and other fields is described.It can be used in vivo and in vitro as medical materials;it has many characteristics as drug carriers,such as water solubility,biocompatibility and so on,so it can extend residence time of drug in specific parts,then improve bioavailability;it can be used as an sunscreen for cosmetics with excellent UV absorption features and safety;after combined with other materials,it can promote growth of fibroblast as a new injectable soft tissue filler material with good biocompatibility;it also can be used as adsorbent,flocculant and separation membrane material for purification of drinking water,recycling of heavy metal ions,et al.Carboxymethyl chitosan has viscosity,which can extend residence time of drug in the nasal cavity and increase contact time of drug and the nasal mucosa,then it improves bioavailability of drug through the nasal cavity,also plays long-lasting effect as sustained and controlled release preparations.关键词：carboxymethyl chitosan;antibacterial activity;film formation;application prospect;moisturizing;drug carrier;cosmetics
- 3
- |
- 3
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: Traditional Chinese medicine(TCM) is quintessence and treasure of Chinese culture,which makes indelible contributions to people's health in our nation,even in the whole of the world.In recent years,with national government level to propose and implement a strategic modernization of Chinese medicine,Chinese medicine into the country increased,collision of thinking,practice test,questions also exposed a lot.After finishing these problems,this article mainly aims at thoughts of orientation of the scientific research of TCM from seven aspects,namely the history of TCM,scientificalness,thoughts of research and go on,which expects to invoke the TCM among colleagues,especially for young scholars toponder,discuss and sort out the orientation of the scientific research into TCM so as to do a devotion to development of TCM jointly.关键词：traditional Chinese medicine;research directions;thoughts of research
- 5
- |
- 3
- |
- 1
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0