最新刊期
卷 20 ， 期 3 ， 2014
- 摘要：Objective: To optimize enzymolysis technology of total saponins in leaves of Notoginseng Radix Et Rhizoma and evaluate antidepressant activity of its enzymatic hydrolysates. Method: Single factors tests were adopted to optimize enzymolysis technology of total saponins in leaves of Notoginseng Radix Et Rhizoma with the conversion rate of ginsenosides as index.Antidepressant activity of enzymatic hydrolysates was evaluated in chronic unpredictable mild stress (CMS) model of mice. Result: Helicase was chosen as invertase,hydrolysis pH 4.5,reaction temperature at 50 ℃,12 h for reaction time. Under these conditions,the conversion rate of ginsenosides was about 50%.After continuous treatments with unpredictable mild stress for 6 weeks,mice showed depression-like behaviors,such as reduced body weight,decrease in locomotor activity and anhedonia,which indicated that CMS model of mice was prepared.Then mice were orally received drugs from week 7 to week 9,body weight and sucrose preference of mice were significantly increased,and locomotor activity levels of mice also improved to some extent.In week 9,compared with the model group,body weight of different (low,middle and high) dose groups of enzymatic hydrolysates and fluoxetine group increased by 9.33%,12.44%,18.13% and15.03%,respectively(all groups showed significant difference);Sucrose preference rates of these four groups improved by 22.64%,19.87%,19.38% and 20.85%,respectively(all groups showed significant difference);locomotor activity levels of these four groups increased by 8.35%,41.30%,13.33% and 12.06% (only the middle group of enzymatic hydrolysates showed significant difference). Conclusion: Enzymatic hydrolysates of total saponins in leaves of Notoginseng Radix Et Rhizoma had a good antidepressant effects on CMS model of mice.关键词：total saponins in leaves of Notoginseng Radix Et Rhizoma;ginsenosides;enzymatic hydrolysates;model of chronic unpredictable mild stress;depression;single factor tests
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发布时间：2024-01-04 - 摘要：Objective: To investigate process parameters and powder characteristics of Pheretima after low temperature pulverization. Method: Pheretima was crushed again by vibration type low temperature ultrafine pulverizer after it was crushed to uniform particle size with high speed grinderof Chinese medicine,sampling every 3 min until all samples out of machine by 25 min,then particle size,specific surface area and porosity of samples were tested. Result: With increasing of grinding time,particle size decreased gradually,then particle size increased after agglomeration of particles;specific surface area and porosity increased firstly and then decreased.Adsorption-desorption curve showed that relative pressure concentrated in 0.8-1.0 when condensation and evaporation occurred;Adsorption quantity of N2 in sample was maximum after crushed 9 min,and it had significant differences with other samples. Conclusion: Pheretima could be crushed to micron level by vibration type low temperaturepulverization,low temperature pulverizationcould guarantee active ingredients in Pheretima unchanged,and it was not easy to gather after crushed to appropriate particle size,which reached particle design requirements of Pheretima in Xiaojin pills.关键词：particle size distribution;specific surface area;porosity;adsorption-desorption isotherms;Pheretima;ultrafine grinding characteristics
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different drying methods on composition and content of volatile oil from Angelicae Sinensis Radix. Method: Volatile oil was extracted by steam distillation,Angelicae Sinensis Radix was dried by dry in the sun,dry in the shade,stoving at 40 ℃ and 60 ℃,chemical constituents in volatile oil were analyzed by GC-MS. Result: Content of volatile oil was the highest in Angelicae Sinensis Radix with 40 ℃ oven-drying(1.074%) and shade drying(1.165%);Forty compounds in volatile oil from Angelicae Sinensis Radix dried different methods were identified;After dried by different methods,main ingredients in volatile oil was basic the same,but the content of each component vary enormously. Conclusion: Different drying methods had marked influence on composition and content of volatile oil from Angelicae Sinensis Radix,40 ℃ oven-drying should be adopted.关键词：Angelicae Sinensis Radix;drying method;volatile oil;GC-MS;component analysis
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发布时间：2024-01-04 - 摘要：Objective: To optimize dilute acid ethanol extraction technology of 1-deoxynojirimycin(1-DNJ) from Mori Folium. Method: HPLC-ELSD was adopted to determine the content of 1-DNJ with mobile phase of acetonitrile-water (90:10) and ELSD drift tube temperature of 40 ℃,flow rate of carrier gas was 3.5×105 Pa.With yield of 1-DNJ as index,based on single factors,response surface methodology was adopted to investigated effects of soaking time,ethanol concentration and pH on extraction technology of 1-DNJ,then test data were analyzed by Design Expert 7.1.6 software. Result: Optimum process conditions of 1-DNJ were as follows:ethanol concentration 66.53%,pH value of 6.66,soaking time 2.73 h;Under these conditions,yield of 1-DNJ was 0.194 6%,which had small difference between theoretical optimal value of 0.100%. Conclusion: This established quadratic multinomial model was accurate and reliable,optimized technology could provide a reference for development and utilization of Mori Folium resources.关键词：1-deoxynojirimycin;HPLC-ELSD;response surface methodology;dilute acid ethanol extraction technology;Mori Folium
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发布时间：2024-01-04 - 摘要：Objective: To discuss effects of different processing methods on in vitro antioxidant activity and inhibiting capacity for tyrosinase of Bombyx Batryticatus extracts. Method: By contrast with raw Bombyx Batryticatus,in vitro antioxidant capacity differences of methanol extracts of different Bombyx Batryticatus processed products were investigated by taking DPPH radicals,hydroxyl radicals,trivalent iron ions reducing ability and mouse liver microsomal lipid peroxidation as indicators;Inhibiting capacity differences of extracts of different Bombyx Batryticatus processed products for tyrosinase were investigated with L-Dopa as substrate;HPLC was adopted to match and determine ascription of new compositions in Bombyx Batryticatus steamed with licorice-juice. Result: When the crude drug concentration in the range of 2-30 g·L-1,DPPH removal rate of steamed Bombyx Batryticatus extract was most minimum but other ones were basically the same;On the crude drug concentration of 20 g·L-1,clearance rates for hydroxyl radical(·OH) of each processed products were maximum of their owns,removing abilities were presented as raw products>licorice-juice steamed products>microwave processed products>bran fried products>steamed products;In the concentration range of 8-80 g·L-1,total reducing abilities of different products were presented as licorice-juice steamed products>microwave processed products>raw products>steamed products>bran fried products;On the concentration of 80 g·L-1,inhibition of all processed products to mice liver microsomal lipid peroxidation were up to maximum of their owns with order of raw products>licorice-juice steamed products>microwave processed products>bran fried products>steamed products;On the concentration of 20 g·L-1,inhibition rates of all processed products for tyrosinase were up to maximum of their owns with subsequence as licorice-juice steamed products>raw products>steamed products>bran fried products>microwave processed products.By comparing with raw products and other processed products,HPLC profiles showed that methanol extracts of licorice-juice steamed products possess new compounds of liquiritin apioside,liquiritin,ammonium glycyrrhetate and other antioxidant components. Conclusion: In vitro antioxidant activity and inhibition for tyrosinase of Bombyx Batryticatus were weakened after being heated.Overall antioxidant activity of licorice-juice steamed Bombyx Batryticatus was not less than raw products on account of active materials in licorice-juice played a key role in the consideration.关键词：Bombyx Batryticatus;processing technology;in vitro antioxidant activity;inhibiting capacity for tyrosinase;diphenyl-generation bitterness acyl radical;hydroxyl radical
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Tangyushu granules. Method: With composite score of dry extract rate and extraction amounts of berberine hydrochloride and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (stilbene glycoside) as index,orthogonal test was adopted to optimize water extraction technology of Tangyushu granules by taking the amount of water,extraction time and times as factors.Contents of berberine hydrochloride and stilbene glycoside were determined by HPLC. Result: Extraction times had significant influence on water extraction effect,but the amount of water and extraction time had not significant influence.Optimum extraction technology of Coptidis Rhizoma from Tangyushu granules was as follows:extracted three times with ten times the amount of water,one hour each time;Dregs of Coptidis Rhizoma and others pieces in the prescription were extracted three times with eight times the amount of water,one and a half hours each times;Average extracting amounts of stilbene glycoside and berberine hydrochloride were 48.40,5.032 mg·g-1,respectively. Conclusion: This optimized extraction process was stable and feasible,which could be extended to industrial production of Tangyushu granules.关键词：Tangyushu granules;orthogonal test;HPLC;berberine hydrochloride;stilbene glycoside;yield of dry extract
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发布时间：2024-01-04 - 摘要：Objective: To prepare Apocyni Veneti Folium pulse compression-coated tablets. Method: Apocyni Veneti Folium pulse tablets were prepared by dry-compression technique with release lag-time and accumulated release rate in 7 h as evaluation indexes,Box-Behnken design was adopted to investigate effects of ratio of CMS-Na to mannitol in core tablet,hydrogenated castor oil to PEG6000 in coating film and coating weight to core weight on prescription process,functional relationship between indexes and factors was established by binomial equation,prescription of Apocyni Veneti Folium pulse compression-coated tablet was optimized by response surface methodology,and optimized prescription for verification. Result: Relationship between indexes(lag-time and accumulated release rate in 7 h) and factors were fitted by binomial equation,regression coefficients of them were 0.979 8 and 0.955 4,respectively.Optimum prescription was as follows:CMS-Na to mannitol(1:1), hydrogenated castor oil to PEG6000(5.38:1) and coating weight to core weight(1:1);Under these conditions,in vitro lag-time of these prepared tablets was about 5 h,then drug released from these tablets within 6-8 h was found to conform to zero-order kinetics and controlled by bulk erosion mechanism.Bias between observed values and predicted values of lag-time and cumulative release rate in 7 h were 3.61% and-1.42%,respectively. Conclusion: These prepared Apocyni Veneti Folium pulse compression-coated tablets had an ideal timing pulse release effect.关键词：Apocyni Veneti Folium;pulse-release tablets;Box-Behnken design-response surface methodology;single factor test;in vitro release
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发布时间：2024-01-04 - 摘要：Objective: To prepare W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion. Method: W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion was prepared by two-step method,with centrifugation retention rates as comprehensive evaluation index,orthogonal test was used to investigate effects of dosages of emulsifier Ⅰ and Ⅱ,the concentration of gelatin solution and reaction temperature on formulation technology,then combined with particle morphology and particle size distribution to screen optimum formulation and its characterization. Result: Optimum prescription process was as follows:emulsifier Ⅰ and Ⅱ amounts all of 8%,gelatin solution concentration of 0.25%,reaction temperature at 70 ℃;Under these conditions,parcle rate of total saponins from Ginseng Radix Et Rhizoma was 71.8% with surface tension of 32.2 mN·m-1 and conductivity of 453 μs·cm-1. Conclusion: These W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion was stable,it could provide a reference for improving oral bioavailability of total saponins from Ginseng Radix Et Rhizoma.关键词：total saponins from Ginseng Radix Et Rhizoma;W/O/W multiple emulsion;orthogonal test;prescription process
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total tannins in Diascoreo cirrhosa. Method: With content and transfer rate of total tannin as indexes,which was detenninated by UV.Single factor tests were adopted to screen extracting method,grinding degree,wetting time and other factors,effects of ethanol concentration,percolation velocity,collected liquid of percolate on extracting technology was investigated by L9(34) orthogonal test. Result: Ethanol percolation method was adopted,its optimum extraction process was as follows:soaked 24 h before percolation,grinding degree of herbs with coarse powder,wetting time 2 h,ethanol concentration of 80%,percolation velocity of 4 mL·kg-1·min-1,collected 8 times the amount of percolation solution. Conclusion: Optimized extraction process was stable and practicable,which was suitable for industrial production of D. cirrhosa tablets.关键词：Diascoreo cirrhosa;extraction process;Orthogonal Design;total tannins;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different penetration enhancers on in vitro transdermal absorption ability of infantile diarrhea gel ointments. Method: Drug transdermal instrument was adopted to investigate percutaneous penetration of infantile diarrhea gel ointments,acceptable solution was selected by single factor test,and then 24 h in vitro total cumulative permeation and cumulative transmittance of evodiamine and retaecarpine in infantile diarrhea gel ointments were investigated after adding different content(1%,3%,5%) azone,oleic acid,propylene glycol.HPLC was adopted to determine contents of evodiamine and retaecarpine by taking acetonitrile-water(51:49) as mobile phase and detection wavelength of 225 nm. Result: Within 24 h,cumulative transmittance of evodiamine and retaecarpine in blank sample was 34.53%,in samples containing different amounts of azone were 33.92%,34.78% and 34.65%,in samples containing different contents of oleic acid were 33.67%,34.57% and 34.57%,in samples containing different contents of propylene glycol were 34.49%,34.61% and 34.53%,respectively. Conclusion: Volatile components from infantile diarrhea gel ointments had promotion role of drug penetration.关键词：infantile diarrhea gel ointments;percutaneous absorption enhancer;evodiamine;retaecarpine;total cumulative permeation;cumulative transmittance
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the sporophore of Laetiporus montanus. Method: The chemical constituents were separated and purified by chromatographic methods after solvent extraction and were identified by spectroscopic analyses (MS and NMR). Result: Eleven compounds were isolated from chloroform extracts and ethyl acetate extracts in the sporophore of L. montanus and were identified as ergosterol peroxide (1), ergosta-7,22-dien-3β-ol (2), cerevisterol (3), ergosterol (4), ergosta-7,22-dien-3-one (5), fomefficinic acid A (6), sulphurenic acid (7), (4E,8E)-N-D-2'-hydroxypalmitoyl-1-O-β-D-glycolpyranosyl-9-methyl-4,8-sphingadienine (8), N-(2'-hydroxytetracosyl)-1,3,4-trihydroxy-2-amino-octadecane (9), nicotinic acid (10) and eburicoic acid (11). Conclusion: The compounds 3, 5, 6, 8-10 are separated from this fungus for the first time.关键词：Laetiporus montanus;chemical constituents;ergosterol;eburicoic acid
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Amydrium hainanense. Method: The chemical constituents were separated and purified by silica gel column chromatography and recrytallization and their structures were determined by physical constants and spectral analyses. Result: Seven compounds were isolated and identified as 5α,8α-epidioxy-ergosta-6,22E-dien-3β-ol(1), axinysterol (2), emodin (3), oleanic acid (4), ursolic acid (5), stigmasterol (6), β-sitosterol (7). Conclusion: All the compounds are isolated from A. hainanense for the first time.关键词：Araceae;Amydrium hainanense;5α,8α-epidioxy-ergosta-6,22E-dien-3β-ol;axinysterol
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of P. clavata (Bak.) Ching. Method: The whole plant of air-dried P. clavata was extracted with 90% EtOH. The extract was suspended in H2O and extracted with petroleum ether, EtOAc and n-BuOH, successively. The compounds were isolated and purified by column chromatography, and identified based on spectral analyses (MS,1H-NMR,13C-NMR). Result: Eight compounds were isolated from P. clavata, and characterized as diploptene (1), hentriacontane (2), octacosyl palmitate (3), eicosyl behenate (4), mangiferin (5), β-sitosterol (6), daucosterol (7) and sucrose (8). Conclusion: Compounds 1-4 were obtained from P. clavata for the first time.关键词：Pyrrosia clavata;triterpene;xanthone;lipid
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发布时间：2024-01-04 - 摘要：Objective: To develop HPLC for the simultaneous determination of four flavonoids in Callicarpa nudiflora. Method: The separation was performed on a Venusil XBP-C18column(4.6 mm×200 mm, 5 μm),the analytes were separated efficiently using the mobile phase consisted of MeOH-H2O in a gradient program. The flow rate was maintained at 1.0 mL·min﹣1.The detective wavelength was set at 350 nm and the column temperature was 25 ℃. Result: There were good linear relationships between the concentrations and the peak area of luteolin-7-O-β-D-glucopyranoside, luteolin, 5,4'-dihydroxy-3,7,3'-trimethoxyflavone and 5-hydroxy-3, 7, 3', 4'-tetramethoxyflavone in the ranges of 0.000 8-0.016 g·L-1(r=0.999 1),0.001-0.032 g·L-1(r=0.999 1),0.004-0.08 g·L-1(r=0.999 2),0.002 4-0.048 g·L-1 (r=0.999 1),respectively. The recoveries were found to be 98.5% with RSD of 1.7%(n=9), 98.6% with RSD of 1.6%(n=9),98.6% with RSD of 1.4%(n=9) and 98.6% with RSD of 1.7%(n=9), respectively. Conclusion: The method is accurate and reproducible for determination of luteolin-7-O-β-D-glucopyranoside, luteolin, 5,4'-dihydroxy-3,7,3'-trimethoxyflavone and 5-hydroxy-3, 7, 3', 4'-tetramethoxyflavone in C. nudiflora.关键词：Callicarpa nudiflora;luteolin-7-O-β-D-glucopyranoside;luteolin;5,4’-dihydroxy-3,7,3’-trimethoxyflavone;5-hydroxy-3, 7, 3’, 4’-tetramethoxyflavone;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a UPLC method for the determination of fingerprint chromatograms of Tongxinluo capsules, help its quality control and evaluate its internal quality. Method: A Waters ACQUIT UPLC® BEH C18column (2.1 mm×100 mm, 1.7 μm) was used and acetonitrile-0.2% phosphoric acid solution as mobile phase at the flow rate of 0.45 mL·min-1with gradient elution;the detection wavelength was set at 203 nm;the column temperature was maintained at 30 ℃. Result: An UPLC method for the determination of fingerprint chromatograms of Tongxinluo capsules was established. It showed 39 common peaks and the similarity threshod was 0.96. Conclusion: The method was proved accurate and repeatable. It can be applied to the quality control of Tongxinluo capsules.关键词：Tongxinluo Capsules;UPLC;fingerprint;similarity
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发布时间：2024-01-04 - 摘要：Objective: To identify the compounds in herb of Eucommia ulmoides by ultra-performance liquid chromatography PDA electrospray ionization tandem mass spectrometry (UPLC-PDA-ESI-MS). Method: The chromatographic separation was carried out at 45 ℃ on a BEH C18 column (2.1 mm×100 mm, 1.7 μm) eluted in the gradient program. The mobile phase consisted of acetonitrile and water (both containing 0.1% formic acid). The detecion of PDA was set between 190 nm and 400 nm. The mass spectra were obtained by Waters ACQUITYTM triple quadrupole tandem mass spectrometer in negative ion mode with ion spray voltage 3 kV. The temperature of the source and desolvation was set at 120 ℃ and 350 ℃ separately. Nitrogen was used as the desolvation gas (650 L·h-1) and cone gas (50 L·h-1). For collision induced dissociation, argon was used as the collision gas at a flow rate of 0.16 mL·min-1. The mass range recorder was set between 100-1 000 m/z. Result: Three lignin, four iridoid and four phenyl propanoid were well separated and identified by the method. Conclusion: The method is simple and rapid for the identification and quality control for the herb of E. ulmoides.关键词：Eucommia ulmoides;lignans;iridoid;phenyl propanoid;UPLC-PDA-ESI-MS
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发布时间：2024-01-04 - 摘要：Objective: To compare differences between phenol-sulfuric acid method and sulfuric acid-UV method to determine polysaccharides content in Qianliang tea. Method: The two methods were employed to determine content of polysaccharides in Qianliang tea after water extracting-alcohol precipitating and sevage assays. Result: The total sugar stand curve equation were Y=4.755 0X-0.029 5(R2=0.990 4)(by sulfuric acid-UV method) and Y=4.770 0X-0.065 1(R2=0.993 1)(by phenol-sulfuric acid method), which showed a good linear relationship range from 0.02 to 0.1 g·L-1. The determination of phenol-sulfuric acid method and sulfuric acid-UV method were 2.00% and 3.21%, which was statistically significance. Conclusion: As a new method, sulfuric acid-UV method could be a stable and simple method to measure the polysaccharide.
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发布时间：2024-01-04 - 摘要：Objective: To identify Donglingcao tablets from different manufacturers by infrared spectroscopy. Method: The infrared spectroscopy of different manufacturers were analyzed by the original spectra; first derivative and second derivative.And it is clustered by calculating the distance between the spectra. Result: The original spectrum of same manufacturers is very simliar, and the similarities of different manufacturers are relatively small. In the second derivative spectrum, the main difference is the N-H stretching vibration absorption at 3 563 cm-1,C-O stretching vibration absorption at 1 158,1 117,1 080,1 037 cm-1 and C=C outside the plane bending vibration absorption at 983,862,680,655 cm-1. The intensity,shape and position of absorption peaks were obviously difference in all those places. The samples of different manufacturers were identified using clustering analysis. Conclusion: Using the infrared spectrum of the second derivative and clustering analysis can be successful in the identification of four manufacturers of Donglingcao tablets. This method is rapid, nondestructive, green and intuitive features.关键词：Donglingcao tablets;infrared spectroscopy;different manufacturers;second derivative;clustering analysis
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of high-temperature heat treatment of Psoralea corylifolia on the activity of tyrosinase. Method: The activity of tyrosinase was detected by measuring the oxidation rate of L-DOPA in vitro. Result: In addition to the low concentration and median concentration in the 30 min, in the same concentration and high-temperature heat time, the higher the temperature was more, the stronger the activation rate on tyrosinase would be. In the same concentration and temperature, the activation rate was not regularly increased or decreased with the increase of heating time. When the mass concentration was 2 g·L-1, and heated treatment 40 min and 20 min at 121 ℃,the activation rate on tyrosinase was the highest, 135.45%±4.29% and 106.97%±3.21% respectively. Conclusion: By controlling appropriate temperature and time, P. corylifolia could enhance the activation of tyrosinase activity.关键词：Psoralea corylifolia Linn.;high-temperature heat treatment;tyrosinase
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发布时间：2024-01-04 - 摘要：Objective: A method for simultaneous determination of five ingredients in Wuzi Yanzong pill was developed by reversed-phase high-performance liquid chromatography (HPLC). Method: HPLC was performed on Angilent Extend C18 column (4.6 mm×250 mm, 5 μm) with a gradient elution system using methanol:ethylactate(10:1)-0.4% phosphoric acid at 30 ℃ and at a flow rate of 1.0 mL·min-1. The eluate was detected by wavelength of 250 nm for schisandrin, deoxyschizandrin, γ-schizandrin and 360 nm for hyperoside and kaempferide. Result: Hyperoside, kaempferide, schisandrin, deoxyschizandrin and γ-schizandrin were baseline separated and their linear ranges were 4.824-482.4, 4.358-87.16, 1.854-185.4, 5.610-112.2,3.850-77.00 ng, respectively. The recovery of the five compounds (n=9) were 101.2%, 98.9%, 101.6%, 101.3%, 97.6%, respectively. Conclusion: The developed method is simple, accurate, and shows good precision and reproducibility. It is could be used for the quality control of Wuzi Yanzong pill.关键词：hyperoside;kaempferide;schisandrin;deoxyschizandrin;γ-schizandrin;HPLC;Wuzi Yanzong Pill
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发布时间：2024-01-04 - 摘要：Objective: To analyze the volatile components from fruit of Vanill planifol. Method: The volatile chemical constituents were extracted and enriched by headspace solid phase micro-extraction (HS-SPME). The chemical components were analyzed by GC-MS. Selected the solid phase micro-extraction fiber head, while optimized the HS-SPME operating conditions. Result: A total of 28 components were separated and identified, accounted for 98.17% of total volatile components. The main component is vanillin (48.28%) and 2-methoxy-phenol (15.54%). Conclusion: This is the first time to adopt HS-SPME combined with GC-MS to analyze the volatile chemical components from fruit of V. planifoli and this study can provide scientific basis for further research and development of volatile chemical constituents of V. planifoli.关键词：Vanilla planifoli Andrews;HS-SPME;GC-MS;volatile chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To develop an UPLC method of determining the characteristic chromatographic profile of Chrysanthemum indicum for controlling the drug quality quickly and accurately. Method: The analysis was performed on an Agilent C18 column (2.1 mm×50 mm, 1.8 μm) with acetonitrile-0.1% phosphoric acid as mobile phase in gradient elution mode.The flow rate was 0.4 mL·min-1 and the injection volume was 1.0 μL with the detection wavelength of 326 nm.The column temperature was set at 25 ℃. Result: The fingerprint of C. indicum was obtained within 12 min, and five peaks in the fingerprint were designed. 15samples were analyzed, and the similarities were 0.871-0.985. Conclusion: The UPLC system enjoys the merits of a higher speed, sensitivity, and resolution, compared with the HPLC system. It can be used to control the quality of Chrysanthemum indicum.
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发布时间：2024-01-04 - 摘要：Objective: Structure and ultrastructure of cultural materials were studied to explore the correlation between the structure and the accumulation of oridonin. Method: High performance liquid chromatography was used to determine the changes of oridonin in vitro Rabdosia rubescens. On the basis of it, the anatomy of cultural materials of R. rubescens were compared using light microscopy, and transmission electron microscopy. Result: The content of oridonin was higher in donor plant (leaf) tissues, but as they began to dedifferentiation into callus, oridonin were absent in the dedifferentiation callus, when the calli initiated organogenesis (bud primordial), it started appearing. As the leaf dedifferentiated into callus the number of chloroplast decreased. At the same time, the content of oridonin was decline until absent. But when the oridonin accumulated, the number of chloroplast increased again and the differentiation of vascular was founded in the cultural materials. Conclusion: The result showed that the number of chloroplast and differentiation of vascular played key role in biosynthetic pathway of oridonin.关键词：Rabdosia rubescens;Oridonin;callus;chloroplast
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发布时间：2024-01-04 - 摘要：Objective: In order to provide theoretical basis for proper utilization of the plants of paris genus in chinese Traditional medicine, the article compared content of paris-Ⅰ, paris-Ⅱ, paris-Ⅵ and paris-Ⅶ from various plants of different species and habits of paris genus. Method: HPLC-UV method was established. Result: The content and compounds of steroid saponin in plants of paris genus existed significant difference from various species. Conclusion: The four kinds of plants of paris genus owed the same chemistry quality. And P. polyphylla var. pubescens and P. violacea can be used as Rhizoma Paridis in Chinese medication.关键词：Rhizoma Paridis;steroid saponin;HPLC;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To establish a RP-HPLC method to determine plasma concentration of geniposide in rats,and compare pharmacokinetic characteristics of geniposide in Reduning injection and geniposide monomer in rats. Method: Twelve male SD rats were divided into two groups randomly,which were injected Reduning injection and geniposide by tail vein,respectively.Orbital blood at different time points,with syringoside as internal standard,plasma was precipitated proteins with acetonitrile and centrifuged at 13 000 r·min-1 for 10 min.RP-HPLC analysis was performed on an Agela C18 column(4.6 mm×150 mm,5 μm) with acetonitrile-water(14:86) as mobile phase,main pharmacokinetics parameters were calculated with PhoenixTM WinNonlin 6.1 software. Result: Geniposide showed good linearity in 0.100-120 mg·L-1 with r=0.998 7,the lowest limit of quantization of geniposide was 0.100 mg·L-1,the mean extraction recoveries of plasma samples with low,medium and high concentration were (85.9±1.7)%,(96.6±2.3)% and (97.4±1.1)%,respectively.Concentration-time profiles of geniposide were fitted with the two-compartment model both for Reduning injection and geniposide monomer,t1/2 were (0.58±0.11) and (0.55±0.20) h,MRT0-t were (0.41±0.05) and (0.33±0.05) h,respectively,main pharmacokinetic parameters had no significant differences between Reduning injection and geniposide monomer. Conclusion: This developed method was sensitive,rapid and accurate for investigating pharmacokinetics of geniposide in rats.Experimental results showed that the other constituents in Reduning injection have no significant impact on pharmacokinetics of geniposide in rats.关键词：Reduning injection;geniposide;RP-HPLC;pharmacokinetics;drug-time curve;syringoside
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发布时间：2024-01-04 - 摘要：Objective: To study the urinary excretion of daphnoretin and glucuronidated daphnoretin in rats. Method: Suspended in solution, daphnoretin was orally administered to rats at the dose of 50, 100, 200 mg·kg-1. At various time intervals, the urine was collected. The urine was treated with β-glucuronidase. The daphnoretin in urine was extracted by acetic ether.The organic phase was removed into the tubes and then dried in pressure blowing concentrator. The residue was dissolved in 200 μL of mobile phase. The solution of 20 μL was drawn and detected by high-perormance liquid chromatography. Result: The cumulative urine excretion of daphnoretin was (23.13±2.36), (41.74±2.02) and (80.01±3.21) μg at the dose of 50, 100, 200 mg·kg-1, respectively. The cumulative urine excretion of glucuronidated daphnoretin was (37.51±4.51), (61.17±3.90) and (131.25±13.85) μg at the dose of 50, 100, 200 mg·kg-1, respectively. Conclusion: The cumulative urine excretion of glucuronidated daphnoretin is more than cumulative urine excretion of daphnoretin. The daphnoretin and glucuronidated daphnoretin are excreted in the manner of linear dose-dependent.关键词：daphnoretin;urinary excretion;glucuronidation;HPLC
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发布时间：2024-01-04 - 摘要：Objective: Using metabonomics method to study the influence of different compatibility proportion of Gastrodia elata and Ligusticum chuanxiong to endogenous metabolites in migraine rats, the purpose of these works was to find potential biomarkers and to analyze the metabolic pathways which can provide new ideas for the pharmacological mechanism of traditional Chinese medicine. Method: The herbs were divided into four groups:G. elata Group, L. chuanxiong group, G. elata and L. chuanxiong A(1:4), G. elata and L. chuanxiong B (4:1). Each group of herbs was extracted with 70% ethanol (10 times the amount of volume). The rat model of migraine was established by injecting nitroglycerin into the organization of rat. The methods of PCA and PLS-DA were used to analysis the influence of different compatibility proportion of G. elata and L. chuanxiong on endogenous metabolites in migraine rat, and search for biomarkers with load map information. Result: The analysis result of metabolomics showed that:compared with G. elata and L. chuanxiong B group, the score points of G. elata and L. chuanxiong A group were more closer to the normal control group, farer from the model group;16 potential biomarkers were selected from the load plot, and 9 potential biomarkers were identified their structure and analyzed metabolic pathways. Conclusion: Metabolomics can provide detection indexes that reflect the metabolic state of the body. G. elata and L. chuanxiong A group can return the endogenous metabolic phenotype of migraine model rat to normal status. Its mechanism may be involved in vivo physiological activity, such as amino acid metabolism and the citric acid cycle.关键词：Gastrodia elata;Ligusticum chuanxiong;drug compatibility;migraine;metabolomics
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Huihui Gansong Yin (HGY) on the early stage of diabetic nephropathy. Method: The model of diabetic nephropathy was developed by high-fat diet combined with streptozocin injections. Rats were divided into normal group,model group, glurenorm treatment group(10 mg·kg-1) and HGY treatment group(5,10 g·kg-1).The serum cholesterol(CHO), triglyceride(TG), high density lipoproteins(HDL) and low density lipoprotein(LDL) were observed. Enzyme linked immunosorbent assay was used to detect GHbA1c and MAU. The kidneys of rats were harvested for hematoxylin and eosin and the periodic acid-sliver dyeing. The renal lesions were observed under light microscope. Result: Compared with the control group, the level of CHO, TG, LDL, GHbA1c and MAU was significantly increased in the model group(P < 0.01), while the level of HDL was significantly decreased(P < 0.01). Compared with model group, the levels of blood lipids on the HGY treatment group and glurenorm treatment group were better(P < 0.01 or P < 0.05), the levels of GHbA1c and MAU were lower(P < 0.01), meanwhile the HGY treatment group was superior to the glurenorm treatment group on the reduction of MAU. The renal lesions of the HGY and glurenorm treatment groups were lightened than the model group, meanwhile the HGY treatment group was superior to the glurenorm treatment group. Conclusion: HGY may be used to ameliorate blood lipids, decrease the level of GHbA1c and MAU, lessen the pathological of diabetic nephropathy. HGY has protective effects on the early stage of diabetic nephropathy.关键词：Huihui Gansong Yin;diabetic nephropathy;protective effects
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发布时间：2024-01-04 - 摘要：Objective: To research the effects of Zhali Nusi fang(ZLNSF) on the permeability changes of blood brain barrier after focal cerebral ischemia-reperfusion in rats. Method: SD rats were divided randomLy into Sham-operated,model,nimodipine and ZLNSF groups. Rat focal cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion with nylon thread.Afte rats were administered orally with corresponding drugs 1,3,7,14 d,then take out brain.The neurological scores were evaluated.Wet and dry weight measured brain water content;immunohistochemical method to measure IgG expression. Result: Compared with the Sham-operated group, ischemia reperfusion brain water content, IgG expression was significantly increased (P < 0.01, P < 0.05), neurological function score significantly dropped (P < 0.01);compared with the model group, the nimodipine group of brain water content, neurological function score emerged varying degrees of improvement (P < 0.05),the expression of IgG level decreased, especially in 3,7 d significantly (P < 0.01);compared with nimodipine group, ZLNSF group brain water content, IgG expression was emerged varying degrees down (P < 0.05), and nerve function integral increases especially in 3,7 d;compared with 1 d,ZLNSF 3,7 d groups the expression of IgG levels were significantly lower (P < 0.05). Conclusion: Cerebral ischemia-reperfusion injury can induced breakdown and increased permeability of the blood-brain barrier after 1-7 d,then the blood-brain barrier permeability gradually restored after seven days;it is indicated that the treatment of the ZLNSF can promote the cerebral protection. Its mechanism may be related to down-regulate the expression of IgG and reduce the permeability of blood-brain barrier, hold back harmful and large molecules from entering the brain, which plays a critical protective effect.关键词：Zhali Nusi fang;cerebral ischemia-reperfusion injury;cerebral protection;blood-brain barrier
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发布时间：2024-01-04 - 摘要：Objective: To compare antioxidative activity of Ginkgo biloba fermentation liquid ethyl acetate extract under different fermentation time. Method: The Eurotium cristatum was inoculated into liquid medium, incubation time were 4,7,11,14 d respectively. The ethyl acetate extract of G. biloba aqueous extract was treated as control. E. cristatum and some residual impurities were filtrated to harvest clear fermentation liquor, and extracted by solvent extractions. Antioxidation of ethyl acetate extract was evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS) and ferric reducing antioxidant power (FRAP) assays. The different activitiesfrom fermentation time were compared. Result: All above extracts showed different antioxidant activities, moreover, 11 d extracts displayed the best activity:DPPH 50% inhibiting concentration(IC50) 105.7 mg·L-1;ABTS IC50 83.6 mg·L-1, which was close to vitamine C(VC) and the results of absorbance in FRAP test were quite consistent. Conclusion: Antioxidative composition accumulated in G.biloba fermentation liquor was largest at 11 d fermentation time.关键词：Ginkgo biloba;Eurotium cristatum;antioxidation;DPPH;ABTS;FRAP
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the influence of Veratrum nigrum L. to Panax gingseng's anti-fatigue effect with the various physiological and pathological conditions. Method: ICR mice were device two groups, the normal and Qi deficiency groups. The Qi deficiency mice were constructed by administrating of Rhei Radix et Rhizoma and on diet. The uniform design method was used by two factors and seven/eleven levels to investigate the hypothermal swim time in the normal and Qi deficiency mice. Result: For normal mice, dose range of P. gingseng's anti-fatigue effect was 1.00-8.00 g·kg-1. When P. gingseng:V. nigrum<100:1, the total dose was 1.00-9.00 g·kg-1, V. nigrum had the antagonism effect to P. gingseng and the influence would enhance with the increasing of dose. But V. nigrum only decreased the potency not the efficacy of P. gingseng. For Qi deficiency mice, dose range of P. gingseng's anti-fatigue effect was 0.50-8.00 g·kg-1. When P. gingseng:V. Nigrum<100:1, the total dose was 0-9.00 g·kg-1, there was a positive correlation between the hypothermal swim time and the dose of P. gingseng. V. nigrum could not affect the anti-fatigue function of P. gingseng. Conclusion: The mice with the various physiological and pathological conditions, dose of P. gingseng's anti-fatigue effect were different, and the influence of V. nigrum to P. gingseng's anti-fatigue effect was different either.关键词：eighteen incompatible pairs;Panax gingseng;Veratrum nigrum;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Hedysari Radix flavonoids in different time points on angiogenesis index in the pulmonary interstitial fibrosis rat model. Method: Hundred and forty-four SPF Wistar rats were distributed to blank group, model group, prednisone group, Hedysari Radix flavonoids high, middle, low dose groups by bleomycin intratracheal instillation method to establish the model of pulmonary fibrosis. From the second day after modeling, the corresponding drugs were given to each group, ig for 14 days or 28 days. Immunohistochemistry method was used to observe the expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR2). Result: Model groups 14, 28 days VEGF and VEGFR2 increased significantly when compared with the control group.Compared with 14 d model group, high dose flavonoids group in VEGF and VEGFR2 expression decreased (P<0.05). Compared with 28 d model group, high dose flavonoids group in VEGF and VEGFR2 expression decreased significantly (P<0.05). Fourteen, 28 d low dose groups had no significant difference with the 28 d model group, in VEGF and VEGFR2 expression. 14, 28 d middle dose groups in expression of VEGF and VEGFR2 were between 14, 28 d high dose group and low dose group, and had no statistical significance with 14 d, 28 d model group. Conclusion: The anti fibrosis mechanism of Hedysari Radix flavonoids was probably through inhibiting angiogenesis related factor VEGF and VEGFR2 to inhibit the formation of pathological angiogenesis resulting in the delayed pulmonary fibrosis.关键词：pulmonary fibrosis;Hedysari Radix flavonoids;neovascularization;vascular endothelial growth factor;vascular endothelial growth factor receptor
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发布时间：2024-01-04 - 摘要：Objective: To study the intervention effects of Mailuoning injection on the nf-kappa B(NF-κB) and tissue factor(TF) pathway of human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS). Method: HUVECs were cultured in a low-glycemic DMEM containing 10% fetal bovine serum, 0.5% penicillin and streptomycin and 10 μg·L-1 basic fibroblast growth factor(bFGF) at 37 ℃. The inflammation cell model of HUVECs was induced by 5 mg·L-1 LPS treatment for 24 hours. The activity of the inflammatory cell model after the treatment of Mailuoning injection was observed by MTT, so as to determine the low, middle, high doses group on Mailuoning injection. And the activities of interleukin-6(IL-6) and tumor necrosis factor alpha (TNF-α) were detected by ELISA. Besides, using Western blotting for detection of NF-κB and TF content. Result: Compared with the model group, effect of Mailuoning in treatment group increased significantly (P<0.05 or P<0.01). But the effect was stable or falling at the dose of 12 mg·L-1, so the low, middle and high dose group of Mailuoning was determined as 4, 8, 12 mg·L-1, respectively.The level of IL-6 and TNF-α and the NF-κB and TF protein expression of the inflammatory cell model were significantly reduced by each dose group of Mailuoning(P<0.01). Conclusion: Mailuoning injection has a certain relationship between the anti-inflammatory effect and its antagonist of NF-κB and TF.关键词：Mailuoning injection;lipopolysaccharide;human umbilical vein endothelial cell;NF-κB;TF
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of ethyl acetate extract from Smilax china on the cholesterol metabolism in monocyte/macrophage cell line(THP-1)-derived lipid-loaded cells,and to investigate the effect of the expression of sterol regulatory element binding protein(SREBP-1) and caveolin-1 in this process. Method: The ethyl acetate extract with different density (0,40,80,160 mg·L-1) were used to detect the level of total cholesterol(TC), free cholesterol(FC), cholesterol ester(CE) by using high performance liquid phase analytic process and the expression of SREBP-1 by Western blot at different time (0,12,24,48 h) in THP-1 derived lipid-loaded cells. Result: The ethyl acetate extract from S.china had some effects on THP-1-derived lipid-loaded cells, the lipid droplet density decreased in cells and the level of TC, FC, CE was decreased with a dose and time-dependent manner. The expression of SREBP-1 was upregulated. Conclusion: The ethyl acetate extract from S.china can decreased the level of TC, FC, CE in THP-1 derived lipid-loaded cells, and it is contributed to the up-regulation of SREBP-1 expression at some degree.关键词：Smilax china;ethyl acetate extract;cholesterol;SREBP-1
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-fatigue effect of Panax ginseng on qi deficiency model mice. Method: Mice were randomly divided into five groups:three treatment groups(P. ginseng orally given for 14 days,2.5,5,10 g·kg-1·d-1),model group and control group. Applying Qi deficiency model mice induced by dietary restriction, P. ginseng was established with the changes of body weight, blood lactic acid, blood urea nitrogen and weight-loaded swimming test with computer-aided automatic control system for measuring swimming in mice. Result: P. ginseng can increase body weight. The level of blood lactic acid and blood urea nitrogen descended. The sink time,the death time, immobility time, swimming time, first sink time, swimming distance and immobility time rate in 10 g·kg-1 group was significantly prolonged and swimming time rate was shortened as compared with model group. Conclusion: P. ginseng has anti-fatigue effect on Qi deficiency model mice. It can enhance endurance of mice by prolonging immobility time rate and shortening swimming time rate.关键词：Panax ginseng;Qi deficiency;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: To compare effects of superfine and common powder of Gastrodia elata on the neuroethology, oxidative stress and inflammatory responses in rats with Parkinson's disease (PD). Method: The rat PD model was established by injection of 6-hydroxydopamine (6-OHDA) into the substantia nigra of right side brain. The mice were divided into the normal group, model group, G.elata common powder of low, medium and high dose groups (0.45, 0.9, 1.8 g·kg-1), and superfine powder of low, medium and high dose groups (0.45, 0.9, 1.8 g·kg-1). The changes of neuroethology, oxidative stress and inflammatory responses in PD rats after treatment with superfine and common powder of G. elata were observed. Result: The rats in PD model group after treatment with superfine and common powder of G.elata appeared different levels of improvement of neuroethology. After treatment, superfine and common powder of G. elata could decrease the levels of malondialdehyde (MDA), nitric oxide (NO) and tumor necrosis factor-α (TNF-α), increase the content of glutathione (GSH), and enhance the activity of glutathione-peroxidase (GSH-Px) and superoxide dismutase (SOD) different levels. Conclusion: Both the superfine and common powder of G. elata has the improvement effect on the neuroethology, oxidative stress and inflammatory responses in rats with PD. Furthermore, the superfine powder is more effective than the common powder in pharmacological action, which provide experimental references for the clinical study of superfine powder of G. elata.关键词：Gastrodia elata;superfine powder;common powder;rats with Parkinson’s disease (PD);comparative study
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Changyanqing decoction on the expressions of sIgA and P-selection on ulcerative colitis rats induced by immune-combined TNBS/ethanol. Method: Sixty-four healthy rats were divided into 2 groups. Normal control group was injected saline and the model group was established with immune-combined TNBS/ethanol. The UC model rats were randomly divided into model group, Changyanqing group (40 g·kg-1), Changyanqing group(10 g·kg-1) and SASP group(0.1 g·kg-1). All model rats were treated for 4 weeks. During the 4-week treatment, disease activity index (DAI) were observed. At the end of treatment, morphological observation was carried out on the rats' colon [colonic mucosal damage index (CMDI) scores and histological score (HS)]. The activity of myeloperoxidase (MPO) was measured by biochemical method;sIgA was determined by ELISA;P-selection was determined by immunohistochemical staining (SP method). Result: DAI, CMDI, HS, MPO in model rats were obviously increased(P<0.01);P-selection was also markedly higher in colon(P<0.01);whereas sIgA was significantly decreased in UC rats compared to normal rats(P<0.01). Compared with the UC model group, Changyanqing(10,40 g·kg-1)DAI, CMDI, HS, MPO were obviously reduced (P<0.01);Compared with the UC model group, Changyanqing(10,40 g·kg-1) sIgA was significantly increased in the rats(P<0.01), whereas P-selection was markedly reduced in the colon (P<0.01). Conclusion: Therapeutic effect of Changyanqing on ulcerative colitis rats is obvious, its mechanisms may be related to deceasing P-selection and enhancing sIgA.关键词：ulcerative colitis;Changyanqing;sIgA;P-selection
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发布时间：2024-01-04 - 摘要：Objective: The total flavonoids of Broussonetia papyrifera(L.) Vent(TFBP) was investigated for its antitumor activity and induction of apoptosis in human hepatocellular carcinoma cell line HepG-2 in vitro. Method: The HepG-2 cells in logarithmic phase of growth were randomly divided into drug group and control group, with the concentration of 3,6,9,12 g·L-1 TFBP in hepatocellular carcinoma HepG-2 cells. The intervention durations was 24,48,72 h accordingly, the viability of HepG-2 cells was measured by MTT. Morphology of cell apoptosis was observed by Hoechst 33342 fluorescence staining. The cell cycle and cell apoptosis rate were detected by flow cytometry. Result: TFBP could inhibit the growth of cells and cause apoptosis significantly.The suppression was in a time-and dose-dependent manner,HepG-2 cells were treated with TFBP of 3,6,9,12 g·L-1 concentrations, the inhibition rates were 20.2%,29.44%,39.21%,43.96% at 48 h. When HepG-2 cells were treated with the concentration of 9 g·L-1 TFBP, the inhibition rate could be achieved 52.46% at 72 h, there was a significant difference compared with control group (P < 0.05) The typical apoptosis characteristics such as nuclear fragmentation and apoptotic body could be observed under the fluorescence microscope.In the same time, with the increase of drug concentration, cell apoptosis rate increased significantly(P<0.01).TFBP could reduce the cell population in G1/G0 phase and increase the cell population in G2/M phase significantly (P < 0.05).TFBP could induce a G2/M cell cycle arrest. Conclusion: TFBP has apparent inhibition and apoptosis-inducing effect on HepG-2 cells. TFBP may induce apoptosis and induce a G2/M cell cycle arrest.关键词：HepG-2 cell line;TFBP;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Tribulus terrestris on the content of testosterone, substance metabolism and anti-fatigue ability of rats. Method: By using the model of high-intensity endurance training,Sixty-five male Wistar rats were divided into 5 groups, 12 in each group, including control group (C group), motion control group (T group), exercise+ig low-dose T. terrestris group (TML group), exercise+ig middle-dose T. terrestris group (TMM group), and exercise+ig high-dose T. terrestris group (TMH group). Gavage was performed once a day. The rats in T. terrestris groups were gavaged with 0.5,1,3 g·kg-1. The rats in C and T groups were given saline of same volume. After 49 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: The body weight of the rats in T. terrestris groups was less than C group (P<0.05), greater than those in T group (P<0.05), and the swimming time was longer than C and T group (P<0.01). The serum testosterone was lower than group C (P<0.05), higher than the T group (P<0.01). Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. Liver glycogen and muscle glycogen were higher than in C and T group(P<0.05 or P<0.01). Blood urea nitrogen(BUN) was higher than C group (P<0.05), lower than the T group (P<0.05);hemoglobin was lower than C group (P<0.05), higher than the T group (P<0.05). Conclusion: T. terrestris can alleviate the impact of high-intensity exercise on serum testosterone, and maintain it at normal physiological levels;it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.关键词：Tribulus terrestris;testosterone;anti-fatigue;luteinizing hormone;follicle-stimulating hormone
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effect of Cujing Tang (CJT) on mouse semen routine, sex hormone and sperm apoptosis. Method: Fourty mice were used to build oligozoospermia and asthenozoospermia model with cyclophosphamide (CTX) 60 mg·kg-1·d-1 for 5 days, and randomly divided into model group,CJT low dose treatment group,middle dose treatment group and high dose treatment group, with 10 rats in each group. In addition, a blank control group was set up. The blank control group and model group received 0.5 mL normal saline per mouse by gavage. After modeling, low, middle and high dose treatment group were given with 2.5, 5, 2.10 g·kg-1 CJT by gavage for 30 days. The quality of mice sperm were detected by CASA, serum sex hormone was detected by ELISA and sperm apoptosis was detected by flow cytometry. Result: After modeling the sperm density, vitality and viability of model group were significantly reduced (P<0.05). CJT could significantly improve mice sperm density, vitality and viability (P<0.05), and high dose goup had no significant difference compared with blank group. follicle-stimulating hormone(FSH) and prolactin(PRL) had no significant difference in all groups. luteotropic hormone(LH), testosterone(T) and estradial(E2) of model group were significantly higher than other groups (P<0.05). The LH, T and E2 were reduced in all CJT group, and especially high dose group had no significant difference compared with blank group. Sperm apoptosis of model group was significantly higher than that of blank group (P<0.05). The apoptosis rate were decreased in all CJT group, especially high dose group, which had no significant difference compared with blank group. Conclusion: CJT can increase sperm density, motility and viability, repaire testicular pathological injury and decrease the apoptosis rate of testicular spermatogenic cell.
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发布时间：2024-01-04 - 摘要：Objective: To observe the apoptosis molecular mechanism of gastric cancer SGC-7901 cells induced by Jiawei Danshen decoction. Method: After gastric cancer SGC-7901 cells treated with Jiawei Danshen decotion for 24, 48, 72 and 96 h, cell growth inhibition rate and apoptosis rate were detected by MTT and flow cytometry. The immunocytochemistry method was used to study the effects of Jiawei Danshen decoction on the expression of Bcl-2 and Bax in SGC-7901 cells. Result: the inhibition rate of SGC-7901 induced by Jiawei Danshen decoction was higher than that of negative control group(P < 0.05). The result of Apoptosis rate showed SGC-7901 cells induced by Jiawei Danshen decoction has a higher apoptosis rate compared with the negative control group(P < 0.05). After treated with Jiawei Danshen decoction, the expression of Bcl-2 was obviously decreased and the expression of Bax was obviously increased. Conclusion: The Jiawei Danshen decoction can induce apoptosis of SGC-7901 cells. The mechanism may be relate to its regulation on the expression of Bcl-2 and Bax.关键词：Jiawei Danshen decoction;gastric cancer cell line;cells apoptosis;Bax;Bcl-2
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of total flavones of Epimedium leptorrhizum Stearn (YYH-C) on rat osteoporosis induced by retinoic acid. Method: Rats were divided into control group, model group, Xianling Gubao capsule group(0.3 g·kg-1)and YYH-C lower, middle, and high dose (raw materials 0.7,1.4, 2.8 g·kg-1) groups. Except the control group, each groups were administrated retinoic acid 70 mg·kg-1 in the first 14 consecutive days. At the same time, the different treatment groups were given the corresponding drug to intervene for 28 days. The vertebrae bone and femur bone of left side were dissected to examine the parameters of bone, including bone mineral density (BMD), bone Ca, bone P, and ash weight/dry weight percentage;the femur bone of right side were dissected to assess the morphology of bone. Result: After 28 days, the BMD of vertebrae bone and ash weight/dry weight percentage of femur bone of the model rats were obviously lower, compared with control group(P<0.01,P<0.05). Ca and P content of bone of the model rats were also significantly lower than those of control group(P<0.01). Morphological examination showed osteoporosis performance such as femoral cortical thinning, bone marrow cavity enlargement, the trabecular bone becaming thin, sparse and irregular, moreover, osteoblast number reduced. YYH-C 2.8 g·kg-1 group could significantly increase the BMD and ash weight/dry weight percentage of vertebrae bone(P<0.01,P<0.05), meanwhile, three treatment groups of YYH-C could increase the ash weight/dry weight percentage and Ca, P content of the bone, enlarge trabecular area and cortical bone area percentage(P<0.01,P<0.05). In addition, YYH-C 1.4, 2.8 g·kg-1 groups could increase the thickness and area percentage of trabecular bone (P<0.01), YYH-C of 1.4 g·kg-1 could reduce the number of osteoclasts (P<0.05). Conclusion: The total flavones of E. leptorrhizum Stearn could resist osteoporosis on rat effectively by preventing the loss of bone mass on rat caused by retinoic acid, improving the microstructure of bone tissue, regulating the disorder of bone formation and bone destruction.关键词：total flavones of Epimedium leptorrhizum Stearn;retinoic acid;osteoporosis
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发布时间：2024-01-04 - 摘要：Objective: To research the antiviral efficacy of ‘kidney deficiency Fuxie’ theory on the treatment of hepatitis B virus e antigen(HBeAg)-positive chronic asymptomatic hepatitis B virus carriers. Method: Under the theoretical guidance of ‘kidney deficiency Fuxie’, we developed the herbal compound Wenbu Toujie prescription. A randomized, control methods, 60 HBeAg-positive chronic asymptomatic hepatitis B virus carriers were randomly divided into a treatment group(35) and the control group(25), the treatment group treated with Wenbu Toujie prescription, the control group was not given any medication, treatment for 48 weeks. Before treatment, 24 weeks, 48 weeks of treatment, collected blood to detect liver function, hepatitis B virus deoxyribonucleic acid(HBVDNA) and HBV markers respectively, and observed the changes of the blood, urine, ECG and other safety indicators. Result: When 24 weeks and 48 weeks, Wenbu Toujie prescription significantly stronger than the control group (P < 0.05, P < 0.01) in reducing serum of HBVDNA (≥2log10 decline),with a time-dependent manner (P < 0.05). Only when 48 weeks Wenbu Toujie prescription stronger than the control group (P < 0.05) in the HBVDNA seroconversion rate, without a time-dependent manner (P < 0.05). Only when 48 weeks,HBeAg seroconversion rate was significantly better than the control group, the difference was statistically significant (P < 0.05), Wenbu Toujie prescription elevated peripheral blood CD4+T cell count, lowered CD8+T cell count (P < 0.05), thereby restoring the proportion of T cell subsets. No significant changes in liver function, renal function, electrocardiogram, suggesting that the party has a good security. Conclusion: Wenbu Toujie prescription has antiviral activity in clinical treatment in HBeAg-positive chronic asymptomatic hepatitis B virus carriers,and safe,prompting the TCM ‘kidney deficiency Fuxie’ theory is feasible and safe on the treatment of HBeAg-positive chronic asymptomatic hepatitis B virus carriers,may be related to elevating CD4+T cells, reducing CD8+T cells.关键词：kidney deficiency Fuxie;chronic asymptomatic hepatitis B virus carriers;Wendu Toujie prescription
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Jinlong Guben mixture on treating asthma in remission stage (Latent phlegm in lung syndrome). Method: One hundred and thirteen patients of asthma on remission stage were randomized to the control group and the treatment group. The treatment group were given Jinlong Guben mixture. The control group were given nothing to be coated, course of treatment were both 120 days and follow-up period were both 360 days. To observe the change of asthma control test (ACT) scores,forced expiratory volume in 1 second(FEV1) and peak expiratory flow(PEF). Result: After treatment,ACT scores,FEV1 and PEF of the treatment group were markedly increased, with statistical significance (P < 0.01),and can have long-lasting and stable effect. There were significant differences in ACT scores and FEV1 of two groups, with statistical significance (P < 0.01). Conclusion: Jinlong Guben mixture has a good curative effect on treating asthma in remission stage (Latent phlegm in lung syndrome).关键词：Jinlong Guben mixture;remission stage;asthma
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Simo decoction combined with lactulose on senile intestinal pseudo-obstruction(SIPO). Method: Forty-six SIPO patients were devided into therapeutic and control groups. The methods of nesteia, intestinal decompression, purgative enema, nutrition support were administered to all patients and meanwhile lactulose oral solution and Simo decoction oral solution were given to the patients of the therapeutic group additionally for 7 days. Result: The total effective rate observed in the therapeutic and control groups were 56.5% and 78.3% respectively after 7 days therapy (P<0.05). The scores of intestinal pseudo-obstruction symptoms observed in the therapeutic group were obviously decreased (P<0.05). Conclusion: Lactulose and Simo decoction may be used to treat SIPO patients.关键词：Simo decoction;lactulose;senile intestinal pseudo-obstruction
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Suhuang Zhike capsule on acute exacerbation of chronic bronchitis and the effect on inflammatory factor. Method: One hundred and twenry patients were randomly divided into observation group (n=60) and control group (n=60). Control group was given amoxil capsule, 0.5 g each time, 3 time each day, and ambroxol hydrochloride tablet, 30 mg each time, 2 time each day, which were taken after meal. Suhuang Zhike capsule was added to observation group on the basis of control group, 3 capsules each time, 3 times each day. The two groups were treated for 10 days. The cardinal symptoms such as cough and symptom scores were recorded. The time when cough, cough up phlegm, wheeze and pulmonary rale disappeared or turned to be mild was recoreded. interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) were tested. Result: The total effective rate of observation group (100%) were superior to control group (83.35%) (P<0.01);the symptom scores and the cardinal symptom such as cough, cough up phlegm, wheeze and pulmonary rale of both groups were descended (P<0.01), and the symptom scores and the cardinal symptom except fever of observation group were lower than control group (P<0.01);the time when cough, cough up phlegm, wheeze and pulmonary rale disappeared or turned to be mild of observation group were less than control group (P<0.01);the level of IL-6,IL-8,TNF-α of both groups were descended than before (P<0.01), and the level of IL-6,IL-8,TNF-α of observation group were lower than control group (P<0.01). Conclusion: Suhuang Zhike capsule can rapidly relieve the cardinal symptoms of patients with acute exacerbation of chronic bronchitis, improve the clinical symptoms, enhance clinical effects. The mechanism maybe relate to the reduction of the level of IL-6,IL-8,TNF-α in serum and the alleviation of inflammatory damage to air passage.关键词：chronic bronchitis;acute attack;Suhuang Zhike capsule;interleukin-6;interleukin-8;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical research and effects on microcirculation of self defense Tongqiao decoction to treatment with sudden deafness. Method: One hundred and twenty cases of patients with sudden deafness were randomly divided into control group(n=60)and observation group(n=60). Control group were treatmented with alprostadil injection of 10 μg/times, intravenous infusion, 1 time each day; methylcobalamin tablets, 500 μg orally, 3 times/d. Observation group were treatmented with additional services Tongqiao decoction, and add or subtract according to different syndromes,1 time each day. Treatment lasted 14 d. The changing circumstances of pure tone threshold were measured with acoustic impedance and pure tone audiometry. The hemodynamics were detected in patients before and after treatment. The improve time of hearing, tinnitus, vertigo, ear fullness were record.The levels of plasma endothelin (ET), serum nitric oxide (NO) were treatmented with radioimmunoassay detected. Result: The total effective rate of mild to moderate hearing (95.15% vs 84.2%) was higher than severe hearing.The average total effective rate of observation group(91.66% vs 77.5%)were better than the control group (77.5%, 55.0%, and 70.0%) (P<0.05);the pure tone hearing thresholds after 7 and 14 days were improved than before treatment.The observation group were impoved than the control group (P<0.01).The hemodynamic parameters after treatment were significantly improved, The levels of fibrinogen (Fb), low shear rate whole blood viscosity were improvement than the control group (P<0.01);The levels of ET and NO of observation group were higher than control group (P<0.01). Conclusion: Tongqiao soup dialectical treatment of sudden deafness can reduce clinical symptoms, improve listening skills, improve clinical efficacy.Its mechanism may be related to the decrease in patients with blood viscosity, increased inner ear blood flow, improve microcirculation inner ear.关键词：sudden deafness;Tongqiao decoction;hemodynamics;endothelin;nitric oxide
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发布时间：2024-01-04 - 摘要：Objective: Observation of warming the kidney and strengthening the spleen Huazhuo oral and Enema of dialectical therapy for stage 3 chronic kidney disease (CKD3) clinical curative effect. Method: Eighty-four cases of CKD3 were randomly divided into control group and study group,each of 42 cases. Two groups were treated with lifestyle intervention, symptomatic treatment. Observation group added the application of warming the kidney and strengthening the spleen Huazhuo prescription, including traditional Chinese medicine(TCM) and syndrome was given Huazhuo prescription enema. Treated for 4 months. Record of blood urea nitrogen (BUN), serum creatinine (Scr), glomerular filtration rate (GFR), uric acid (UA), 24 h urinary protein (Upr), serum albumin (Alb), triglyceride (TG), total cholesterol (TC), red blood cell count (RBC), hemoglobin (Hb) changes the main symptoms, physical signs score record. Result: The curative effect to total efficiency in study group was 71.4%, more than that of control group 47.6% (P<0.05); the curative effect of TCM syndrome group total effective rate in study group was 78.6%, more than 57.1% of control group (P<0.05);Scr in study group was decreased compared with that before treatment, and lower than that of the control group (P<0.01);control group Upr continued to rise, the study group Upr decreased after treatment, the study group than in the control group (P<0.01);study group UA decreased, and lower than that of the control group (P<0.01);control group eGFR continued to decline, after the treatment were observed in group eGFR than that in the control group (P<0.05);the observation group TG and TC were decreased than that before treatment, and lower than that of the control group (P<0.05). Conclusion: The treatment of warming the kidney and strengthening the spleen Huazhuo prescription after 4 months improved renal function in CKD3 patients, alleviate the clinical symptoms, delay the progress of CKD3 phase, which is worthy of clinical use.关键词：stage 3 chronic kidney disease;deficiency of spleen and kidney and spleen kidney yang deficiency syndrome;warming kidney and invigorating spleen Huazhuo prescription;renal function
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发布时间：2024-01-04 - 摘要：Objective: To study the clinical curative effect of Xiaozhong Zhitong cream in the treatment of acute soft tissue injury caused by campaign and impact on hemorheology. Method: Eighty cases with acute soft tissue injury induced by sports were randomly divided into control group and observation group, 40 cases in each. Control group adopted Votalin emulgel, observation group received treatment of Xiaozhong Zhitong cream, the course last for 5 days. Clinical symptoms and signs such as pain, swelling, tenderness, ecchymosis, integral function obstacle were observed, before and after treatment, hemorheology indexes, blood sedimentation and c-reactive protein were detected before and after treatment. Result: Pain, tenderness, swelling, ecchymosis and dysfunction scores of observation group were significantly lower compared with that of control group after treatment (P<0.01). The effective rate of observation group was 87.5%, which was higher than 42.5% in control group (P<0.01). Hemorheology indexes such as whole blood viscosity, plasma viscosity, fibrinogen, erythrocyte deposited of observation group were significantly improved than control group (P<0.01). Blood sedimentation and c-reactive protein levels of observation group were lower than that of control group (P<0.01). Conclusion: Xiaozhong Zhitong cream can promote blood circulation and remove blood stasis, and obviously reduce acute soft tissue injury caused by movement.关键词：acute soft tissue injury;sports;Xiaozhong Zhitong cream;blood rheology
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发布时间：2024-01-04 - 摘要：Objective: To study the intervening effects on functional dyspepsia plus depression treated with Jiawei Sinisan, and the gastric emptying, motilin and gastrin were studied to demonstrate mechanism. Method: Ninety-six functional dyspepsia patients were randomly distributed into two groups, the control group treated with domperidone and St. John's wort extract, therapy group treated with taking orally Jiawei Sinisan, both course of treatment were 30 days, gastric emptying, motilin and Gastrin were studied before and after intervention,then general effect was evaluated. Result: The total effective rate was 93.75% in the therapy group whereas 77.08% in the control group, differences were significant(P < 0.05).Compared with control group,the motilin, gastrin and gastric emptying were better in therapy group, differences were significant(P < 0.05). Compared with control group, hamilton depression scale score better in the therapy group,differences were significant(P < 0.05). Conclusion: The effect of Jiawei Sinisan is certain, it could relieve clinlcal symptom of functional dyspepsia.关键词：functional dyspepsia;depression;Jiawei Sinisan;treatment
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发布时间：2024-01-04 - 摘要：Objective: To analyze the composition principles of the prescriptions for Parkinson's disease (PD) by traditional Chinese medicine inheritance support system(TCMISS). Method: The 94 prescriptions for PD in dictionary of Chinese medicine prescription (DCMP) and Pujifang were collected and recorded into the TCMISS (V2.0.1) to build the database. The composition principles of prescriptions were analyzed by revised mutual information, complex system entropy clustering and unsupervised hierarchical clustering methods in the TCMISS. Result: The frequency of each herb included in the database was computed,32 core combinations and 9 new prescriptions were mined from the database base on the 94 prescriptions for PD. Conclusion: The composition principles of the prescriptions treated PD were clarified by the TCMISS, which provided the reference for the clinical application and new drug research in the PD treatment.关键词：Parkinson’s disease;traditional Chinese medicine inheritance support system (TCMISS,V2.0.1);composition principle of prescription
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发布时间：2024-01-04 - 摘要：Objective: To explore the Chinese medicine for primary biliary cirrhosis's regularity and provide the prescription of clinical medicine for primary biliary cirrhosis. Method: Through arranging and researching,we classify the drugs in the literature of primary biliary cirrhosis and use statistical software to describe statistics. Result: The most frequently used Chinese medicine for the treatment of primary biliary cirrhosis are Ariemisiae Scopariae Herba,Glycyrrhizae Radix et Rhizoma,Paeoniae Radix Rubra,Poria,Salviae Miltiorrhizae Radix et Rhizoma,Astragali Radix,Angelicae Sinensis Radix,Atractylodis Macrocep halae Rhizoma.According to frequency of classified Chinese medicine Buxu drugs is the most frequently used, followed by Huoxue drugs and Lishi drugs. Conclusion: The Chinese medicine treat for primary biliary cirrhosis has its own laws and characteristics. In the treatment of primary biliary cirrhosis, attention should be payed to the using of Buxu drugs, Huoxue drugs and Lishi drugs. Meanwhile, joining some drugs which have been shown to have choleretic jaundice facts will have more effective treatment for primary biliary cirrhosis.关键词：primary biliary cirrhosis;Chinese medicine;regularity;literature research
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发布时间：2024-01-04 - 摘要：Objective: To systematically review the effectiveness and safety of Qishen Yiqi dropping pills for ischemic heart failure. Method: Randomized clinical trials concerning Qishen Yiqi dropping pills for ischemic heart failure were searched through CNKI, VIP,WANFANG,PubMed, the Cochrane library from inception to 2013 February. The qualities of included trials were evaluated with the tool of Cochrane method for intervention and the meta-analyses were performed with RevMan5.0.2 software. Result: Ten trials involving 1 070 patients were included. The results of Meta-analyses showed that the combination of Qishen Yiqi dropping pills and western medicine therapy could effectively improve the left ventricular ejection fraction [WMD=0.07,95%CI(0.05,0.08),P<0.000 01] and increase the six-minute walking distance [WMD=48.31,95%CI (39.51,57.10),P<0.000 01], with significant differences compared with the simple western medicine treatment. Qishen Yiqi dropping pills could lower the level of B-type natriuretic peptide. Conclusion: Qishen Yiqi dropping pills could improve the heart function in patients with ischemic heart failure. However, due to the small scale and low quality of included RCT, there was not enough evidence for the popularization and application of Qishen Yiqi dropping pills.关键词：Qishen Yiqi dropping pills;ischemic heart failure;systematic review;randomized clinical trial (RCT);Meta-analysis
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发布时间：2024-01-04 - 摘要：To summarize the differentiation research progress of traditional Chinese medicine(TCM) inducing bone marrow mesenchymal stem cells to neural cells.First author searched the literatures from CNKI database,Articles Database and PubMed database of the last 10 years.The key words were ‘Traditional Chinese Medicine,Bone Marrow Mesenchymal Stem Cells,neural cells,differentiation’in Chinese and English. The retrieval type was and in Chinese and English. A total of 630 articles in Chinese,363 articles in English were obtained and 39 articles were included accoding to the literature selection criteria,removed repeated and non-standard articles. Among the researches of TCM inducing bone marrow mesenchymal stem cells to neural cells differentiation,most are the researches of compound preparations,the single taste and effective compounds or parts of TCM,and provide a certain theoretical basis for TCM that used as an inducer of preventing and treating nervous system diseases,such as ischemic cerebral disease.关键词：traditional Chinese medicine;bone marrow mesenchymal stem cells;neural cells;differentiation
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