最新刊期
卷 21 ， 期 12 ， 2015
- 摘要：Objective: To investigate relationship between osmosis pressure and conductivity of Chinese medicine decotion,and construct model of them. Method: This model was based on the concentration of decotion,which had a postive effect on osmosis pressure and conductivity in some degree.There were 25 system to research relationship between osmosis pressure and conductivity.Rationality of the model was verified by taking Gardeniae Fructus decotion and Liuwei Dihuang Wan decotion as experimental subjects. Result: They had good linear relation in the range of 0.78-7.26 ms·cm-1 of conductivity,the correlation coefficient was 0.964 2.As time went on,the change rule of osmosis pressure and conductivity was similar,in addition,they both had positive relationship with decotion concentration.Fitting equations of Gardeniae Fructus decotion (25-250 g·L-1) and Liuwei Dihuang Wan decotion (25-125 g·L-1) were π=0.088 0e-0.050 1 and π=0.368 4e-0.118 9.These calculated values and measured values were no significant difference. Conclusion: The model of osmosis pressure and conductivity building in this study is proved to be efficient in some degree.It provides a simple,efficient and reliable way to detect osmosis pressure on line in reverse osmosis membrane of Chinese medicine decotion.关键词：osmosis pressure;conductivity;relativity model;Gardeniae Fructus;Liuwei Dihuang Wan;water extract
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发布时间：2024-01-04 - 摘要：Objective: To optimize ethanol extraction process of Fuxiong cataplasms. Method: With dry extract yield and contents of emodin,baicalin,morusin as indexes,orthogonal design was carried out for optimizing extraction process by taking the concentration and amount of ethanol,extracting time and times as factors,optimum process was determined by taking composite score for condition.Contents of emodin and baicalin were determined by HPLC,mobile phases were consisted of methanol-0.4% phosphoric acid (80: 20) and (47: 53), detection wavelengths were 254 nm and 280 nm,respectively.The content of morusin was determined by UV spectrophotometry. Result: Optimum extraction technology was as follows:extracted twice with 6 times the amount of 75% ethanol for 3 h of each time.Dry extract yield and contents of emodin,baicalin,morusin were 31.19%,0.19%,3.61% and 0.51%,respectively. Conclusion: This optimized extraction technology is rational with high extraction rates of active components.关键词：Fuxiong cataplasms;emodin;baicalin;morusin;multi-index comprehensive scoring method
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining total diterpenoids in Platycladi Semen,investigate relationship of contents of fatty oil and total diterpenoids in Platycladi Semen,analyze influence of processing methods on quality of Platycladi Semen. Method: Vanillin-perchloric acid colorimetry was employed to determine the content of total diterpenoids,the content of fatty oil was determined by petroleum benzin.Platycladi Semen was processed by steaming,frying and frosting. Result: Total diterpenoids showed a good linear relationship in 0.011 7-0.058 3 g·L-1,RSD of recovery rate was 0.7%.Relationship between fatty oil and total diterpenoids was 87%.With steaming and frying method,contents of fatty oil and total diterpenoids significantly increased,but they decreased obviously by frosting method. Conclusion: Relationship between fatty oil and total diterpenoids in Platycladi Semen is a strong correlation.The content of grease composition increases with steaming and frying method,but they decrease by frosting method,and this method can reduce the side effect of Platycladi Semen.关键词：Platycladi Semen;total diterpenoids;colorimetry;fatty oil;processing methods
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发布时间：2024-01-04 - 摘要：Objective: To compare quality stability between compressed pieces and traditional pieces. Method: With Menthae Haplocalycis Herba,Perillae Folium,Polygoni Multiflori Caulis and Citri Reticulatae Pericarpium as research objects,long term stability test and accelerated stability test were used to study changes of quality stability between compressed pieces and traditional pieces by determining appearance,TLC,water content,volatile oil,content of pulegone,hesperidin and 2,3,5,4'-teterahydroxy stilbene-2-O-β-D-glucoside(TSG).Mobile phase of pulegone,TSG and hesperidin consisted of methanol-water (72: 28),acetonitrile-water (18: 82),methanol-acetic acid-water (30: 4: 66),detection wavelengths were 252,320,283 nm,respectively. Result: After 12 months or 6 months,the content loss rates of pulegone in traditional pieces and compressed pieces were 26.62%,17.99%;TSG were 6.52% and 4.35%;hesperidin were 7.59% and 4.33%,respectively.Volatile oil from Perillae Folium and Menthae Haplocalycis Herba,the content of pulegone showed significant differences between compressed pieces and traditional pieces,but these other indexes showed no significant differences. Conclusion: Compared with traditional pieces,compressed pieces not only do not change quality of index components to ensure safety,effectiveness and stability of clinical medication,but also provide a new idea for storage of volatile constituents.关键词：compressed pieces;stability;volatile oil;pulegone;2,3,5,4'-teterahydroxy stilbene-2-O-β-D-glucoside;hesperidin
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发布时间：2024-01-04 - 摘要：Objective: To optimize the formulation of tripterygium glycosides loaded gel paste. Method: With the overall weighted score of initial adhesion,peel strength and sensory (appearance, skin adhesive ability and residue of gel paste on the skin after peeling off) as index, the influence of contents of dihydroxyaluminum aminoacetate,glycerol,tartaric acid povidone K90 on characteristics of formulation were evaluated by undertaking experiments according to orthogonal design. Result: All the evaluated factors,especially the content of glycerol as well as dihydroxyaluminum aminoacetate,showed significant influence on characteristics of formulation. In the optimum formulation, the weight ratio of povidone K90 to dihydroxyaluminum aminoacetate, glycerol and tartaric acid was 1.50: 0.15: 30.0: 0.15.The weighted scores of initial adhesion, peel strength and sensory of the optimum formulation were 48.89,19.43 and 28.67,respectively.The prepared tripterygium glycosides loaded gel paste was uniform in size and color,exhibited good skin adhesive ability and no residue on skin after the peeling off of the gel paste. Conclusion: By using orthogonal experimental design,the formulation of glycosides loaded gel paste might be optimized effectively with reduced test times,and these factors influencing characteristics of formulation might be evaluated comprehensively.This optimized formulation is feasible and reproducible.关键词：tripterygium glycosides;gel paste;formulation optimization;comprehensive weighted score
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发布时间：2024-01-04 - 摘要：Objective: To extract high quality genomic DNA from dry leaves of Artemisia rupestris dried naturally and lie a foundation for further molecular experiments. Method: Three different methods,conventional hexadecyl trimethyl ammonium bromide (CTAB) method and modified CTAB methods were applied to isolate genomic DNA from dry leaves of A. rupestris.Two modified CTAB methods were different from conventional CTAB method in three ways,nucleus lysate(STE) was used for removal of impurities,polyvinyl pyrrolidone (PVP) was added when grinding samples and 95% ethanol for precipition of DNA in room temperature.These two modified CTAB methods were different from whether liquid nitrogen was added while grinding samples.Purity and integrality of DNA were detected by UV spectrophotometry,agarose gel electrophoresis and sequence-related amplified polymorphism (SRAP). Result: Higher purity DNA was obtained by improved CTAB methods by comapred with conventional CTAB method,value of A260 nm/A280 nmwas between 1.8-2.0,polysaccharides was removed relatively clear.SRAP produced clear polymorphic patterns. Conclusion: These two modified CTAB methods are suitable for DNA extraction from dry leaves of A. rupestris,purity and integrality of isolated DNA can be used in further molecular biology research.关键词：Artemisia rupestris;dry leaves;DNA extraction;modified CTAB method;agarose gel electrophoresis
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the petroleum ether extract of the root barks of Celastrus angulatus. Method: Petroleum ether extraction was dissolved in 80% methanol and extracted with petroleum ether. The compounds were isolated and purified by column chromatography, recrystallization and preparative HPLC. Their structures were identified by NMR spectra and references. Result: Seven compounds were obtained and identified as (+)-catechin(1), (-)-epicatechin(2), 3β-hydroxyolean-9,12-diene(3), β-sitosterol(4), oleic acid(5), triptohypol F(6), and wilforsinine B(7). Conclusion: Compounds 5,6,7 were isolated from the root barks of C. angulatus for the first time. Compounds 6 and 7 were isolated from genus Celastrus for the first time.关键词：Celastrus angulatus;chemical constituents;triptohypol F;wilforsinine B
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents of volatile oil from the xylem and bark of Acronychia pedunculata in Hainan and its antibacterial activity and cytotoxicity. Method: The volatile oil was extracted by water-steam distillation and analyzed by GC-MS. The relative contents of the compounds were determined by normalization. The compounds were characterized by NIST05 and WILEY275L database matching. Antibacterial activities of the xylem and bark oil were assayed by the filter paper disc agar diffusion and the cytotoxicity of them was evaluated by MTT. Conclusion: 77 compounds were identified from the xylem oil which constituting 67.74% of the total oil and its main constituents were palmitic acid(18.84%), α-copaene (7.94%),δ-cadinene (3.71%), (E,Z)-2,4-decadienal (3.45%) and alloaromadendrene (3.30%). 75 compounds were identified from the bark oil which constituting 96.25% of the total oil and its main constituents were α-pinene (46.70%), α-copaene (19.81%), δ-cadinene (5.80%), alloaromadendrene (4.46%), and limonene (3.53%). 39 compounds were identified both from the xylem oil and bark oil. The volatile oil from the bark showed antibacterial activity against Staphylococcus aureus, but the oil from xylem was inactive. The oil from xylem and bark showed significant cytotoxicactivities towards chronic myelogenous leukemia (K562), human hepatoma(SEL-7402) and human gastric carcinoma (SGC-7901).The activity of the former was better than the latter.关键词：Acronychia pedunculata;volatile oil;GC-MS;antibacterial activity;cytotoxicity
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发布时间：2024-01-04 - 摘要：Objective: To build UV fingerprint of different polar parts from root and stem of Gentiana rigescens and analyze difference of spectral characteristics among eight cultivation patterns. Method: UV data of samples was finished by UV spectrophotometry,data was processed by four print smooth and first orders.Similarly evaluation of UV fingerprint of G. rigescens medicinal materials from different cultivation patterns was finished by common peak ratio and variation peak ratio dual index sequence analysis.Discriminant analysis was used to evaluate discriminate ability of UV fingerprint data for cultivation pattern of G. rigescens. Result: Fingerprint of samples from eight cultivation patterns were lower similarly at 221-247 nm.Chemical polarity fingerprint of different samples,cultivated by same method,was different too.There were significant fingerprint characteristic.UV fingerprints were similar between G. rigescens which grown on slope of valley and which cultivated under Chaenomeles sinensis.However,chemical compounds were significant different between plant grown on slope of valley and which cultivated under Alnus nepalensis.Discriminant analysis showed that about 90% sample could be discriminated according to cultivation patterns. Conclusion: UV fingerprint of different polar parts from root and stem of G. rigescens can help to discriminate cultivation patterns of medicinal materials.关键词：ultraviolet fingerprint;discriminant analysis;cultivation patterns;Gentiana rigescens;quality control
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发布时间：2024-01-04 - 摘要：Objective: To establish a dual-wavelength HPLC method for simultaneous determination of five constituents (chlorogenic acid, resveratrol, rutin, quercetin, kaempferol) in Jingangteng soft capsule. Method: The Agilent ZORBAX SB-C18 column (4.6 mm×150 mm, 5 μm) with a column temperature of 25 ℃ was used;the mobile phase was acetonitrile-0.1% phosphoric acid at gradient elution program and with a flow rate of 1.0 mL·min-1, and the detection wavelengths were set at 306 nm (chlorogenic acid and resveratrol) and 365 nm(rutin, quercetin and kaempferol). Result: The linear response ranges of chlorogenic acid, resveratrol, rutin, quercetin, and kaempferol were 0.058 5-2.34 μg(r=0.999 8), 0.025 3-1.01 μg (r=0.999 9), 0.042 2 -0.844 μg (r=0.999 6), 0.018 1-0.722 μg(r=0.999 9), and 0.016 5-0.660 μg (r=0.999 9), respectively. The average recoveries were 98.0%, 100.6%, 96.0%, 102.7%, and 98.5% with RSDs of 1.2%, 2.2%, 1.8%, 2.9%, and 1.3%, respectively. Conclusion: The developed method was convenient and reliable, which could be applied to determination of five effective constituents in Jinggangteng soft capsule.关键词：Jingangteng soft capsule;chlorogenic acid;resveratrol;rutin;quercetin;kaempferol;HPLC;dual-wavelength
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发布时间：2024-01-04 - 摘要：Objective: To analyze the volatile compounds in Scutellaria barbata from different places. Method: A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) is described for the analysis of volatile compounds in S. barbata from Jiangsu, Guangxi, Jiangxi, Hunan and Sichuan provinces. The volatile compounds were extracted by PDMS/DVB SPME fiber. The relative content of each component was calculated by the normalization method. Result: There are 45 compounds identified in total and 13 volatile compounds in common from the five places mentioned above. The 13 volatile compounds are 1-octen-3-ol,3,7-dimethyl-1,6-octadien-3-ol methyl ester, phenylethyl alcohol, azulene, 2-methoxy-3-(2-propenyl)-phenol,1,2-dimethoxy-4-(2-propenyl)-benzene,pentadecane, propanoic acid, 2-methyl-, 1-(1,1-dimethylethyl)-2-methyl-1,3-propanediyl ester,hexadecane, 6,10,14-trimethyl-2-pentadecanone, hexadecanoic acid methyl ester, dibutyl phthalate, n-hexadecanoic acid. Conclusion: The 13 volatile compounds were identified as the marker components in S. barbata. The results also suggest that there are some differences in the volatile compounds in S.barbata from different places.关键词：Scutellaria barbata;volatile compounds;HS-SPME-GC-MS
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发布时间：2024-01-04 - 摘要：Objective: This thesis focuses on the qualitative and quantitative analysis of the seed oil fatty acid ingredients in different varieties (strains) of Angelica sinensisduring different ripening periods. Method: The seeds oil was obtained by solvent extraction, the oil composition were analyzed by GC-MS. Result: Total of elevenfatty acids were identified with MassworksTM software analysis and library search. Principal component analysis was carried out on the samples, and caproic acid,oleic acid and decanoic acid were identified as the shared characteristic fatty acids of different A. sinensis varieties (strains) during different ripening periods. The samples can be classified using systematic cluster analysis. Conclusion: It was determined that optimal harvest time for the seeds of angelica producersis during August 22 to August 30.Mingui 3 and Mingui 4 are good varieties for further research and promotion.关键词：Angelica sinensis seed;cultivars;different ripening periods;fatty acid;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: Separation and purification of osthole by high-speed counter-current chromatography (HSCCC) from crude extract of Angelicae Pubescentis Radix. Method: Ultra high performance liquid (UPLC) was used to analyze and optimize the solvent system and the hexane-ethyl acetate-water-methanol (1.5: 2.5: 2.0: 1.5) was chosen as the two-phase solvent system, in which the upper phase was used as the stationary phase, while the lower phase was used as the mobile phase, the flow rate was set at 3.0 mL·min-1, the revolution speed was 850 r·min-1, and detected at 323 nm. The fraction was received and the solvent evaporated at 50 ℃ using a rotary evaporator, then the osthole was received and its purity was determined through UPLC, and its structure was identified by IR, ESI-MS,1H-NMR and 13C-NMR. Result: We got 6.6 mg of osthole one time and its purity was up to 97.1%. Conclusion: The high-speed counter-current chromatography is a simple and high-efficiency method and through which we can obtain osthole with high purity from crude extract of Angelicae Pubescentis Radix.关键词：high-speed countercurrent chromatography;Radix Angelicae Pubescentis;osthole
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发布时间：2024-01-04 - 摘要：Objective: To study active constitunents of secondary metabolites in endophytic fungus from Cephalotaxus fortunei root. Method: Antibacterial and antitumor activity of ethyl acetate extracts from fermentation liquor of an endophytic fungus (CEP18) in C. fortunei root were tested by filter paper diffusion method and MTT,active ingredients were analyzed by GC-MS. Result: Secondary metabolites in endophytic fungus from C. fortunei root significantly inhibited growth of Bacillus subtilis,Staphylococcus aureus and Human promyelocytic leukemia cells (HL-60).After silica gel column chromatography analysis and activity test,five components (Fr1-Fr5) were isolated from ethyl acetate extracts,the Fr2 was identified as active component,which was mainly composed of butyl lactate (2.96%),2-chloro ketone warfarin (8.65%),(Z)-11-eicosenamide (13.90%),1-methyl pyrene (3.51%),dimethyl succinate (6.34%),4-hydroxyphenylacetic acid (8.27%),elaidic acid-methyl ester (10.07%),linoleic acid (5.60%) and hexadecanoic acid (7.54%). Conclusion: 4-Hydroxyphenylacetic acid is reported for the first time in C. genus at present research and is worthy of further development and utilization.关键词：Cephalotaxus fortunei;endophytic fungus;secondary metabolites;4-hydroxyphenylacetic acid
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发布时间：2024-01-04 - 摘要：Objective: To investigate application of micro-emulsion thin layer chromatography (ME-TLC) in separation and identification of flavonoids in Shujin Dingtong capsules and Su's Jiegu capsules. Method: Using microemulsion as developing agent in combination with polyamide film as the stationary phase,factors for microemulsion resolution,such as types and concentrations of surfactants,co-surfactants,the oil phase,impact of moisture and organic modifier were investigated by adjusting composition of each component in microemulsion system to find the best TLC conditions for separation and detection of flavonoids in Shujin Dingtong capsules and Su's Jiegu capsules. Result: Naringin in Shujin Dingtong capsules were separated and identified simultaneously with developing agent of micro-emulsion form-acetic acid (9: 1),micro-emulsion was sodium dodecyl sulfate (SDS)-n-butyl alcohol-n-octane-H2O (3.4: 7.3: 2.0: 75.0).Hyperin in Su's Jiegu capsules was separated and identified simultaneously with developing agent of micro-emulsion form-acetic acid (9: 1),micro-emulsion was SDS-n-butyl alcohol-n-heptane-H2O (2.7: 6.3: 1.0: 75.0).Compared with the organic developing solvent,separation effect of micro-emulsion was improved. Conclusion: METLC is a new and efficient method for separation and identification of flavonoids in Shujin Dingtong capsules and Su's Jiegu capsules.关键词：microemulsion;thin layer chromatography;naringin;hyperin;Shujin Dingtong capsules;Su's Jiegu capsules
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发布时间：2024-01-04 - 摘要：Objective: To establish fingerprint of total alkaloids from Isatidis Radix by HPLC. Method: An HPLC fingerprint has been established by gradient elution of acetonitrile and 0.1% H3PO4 aqueous solution, using a Phenomenex column(4.6 mm×250 mm, 5 μm). Detection wavelength was set at 220 nm,Column temperature was not controlled, flow rate was 1.0 mL·min-1, injection volume was 10 μL. The fingerprints were compared with similarity evaluation software published by the 'Similarity Evaluation of Chromatographic Fingerprint of Traditional Chinese Medicine' (2004 A). Result: HPLC fingerprints of alkaloids were established through 12 batchesof Isatidis Radix,and 19 common peaks were identified. Conclusion: This method has been proven to be feasible for the identification of Isatidis Radix.The method is simple, specific, stable, and repeatable, which will provide a methodological basis of quality control of alkaloids from Isatidis Radix.关键词：Isatidis Radix;alkaloids;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To study the fragmentation pathways of forsythoside A and forsythin by electrospray ionization mass spectrometry. Method: A syringe pump was used for the direct roop injections of reference compounds.Forsythoside A and forsythin were analyzed using LTQ orbitrap MS in positive ion mode.The first and multi-stage mass spectrum diagrams of forsythoside A and forsythin were obtained. Result: These were the fragmentation characteristics in the positive LTQ orbitrap MS. Forsythoside A was easy to lose C6H10O4 (rhamnose) or C9H8O4 (caffeicacid), in the MS3 spectra there were two kinds of different sources of m/z 321 ion at the same time,We inferred that fragment ions were from m/z 501 or m/z 467 ion respectively losing caffeic acid or rat lee sugar cracking.Forsythin was easy to lose C6H10O5 (glucose),in multi-stage fracture it was easy to lose H2O. We inferred that ester and glycosidic bond and hydroxyl may contribute to it in the molecular structure. Conclusion: It first reported the fragmentation pathways of forsythoside A and forsythin by LTQ Orbitrap-high resolution mass spectrometer in positive ion mode. The characteristic fragment ions were can be recognized as a rapid identification features of these compounds.Meanwhile,the study can provide accurate data support for further research of metabolites and modifying structure of these compounds.关键词：LTQ Orbitrap;forsythoside A;Forsythin;high resolution mass spectrometry;fragmentation pathways
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发布时间：2024-01-04 - 摘要：Objective: To investigate metabolites and metabolic dynamics of naringin by human intestinal microflora. Method: Human intestinal microflora were obtain from human feces,and incubated with naringin under anaerobic environment.HPLC was applied to monitor the concentration of naringin with mobile phase of methanol-0.1% phosphoric acid solution for gradient elution and detection wavelength at 250 nm.Metabolites were analysis by HPLC-MS. Result: Naringenin,one metabolite of naringin,was detected after incubation for 15 min,which was identified by HPLC-MS and its retention time was about 23 min in HPLC.Metabolism percentage of naringin was reached 100% after incubation for 120 min.Metabolic dynamics of naringin by human intestinal microflora was fitted with nonlinear kinetics.Naringenin could be converting to its metabolite through HPLC monitor. Conclusion: Naringin can be quickly metabolism by human intestinal microflora,its main metabolite is naringenin,secondary metabolites of naringin may be existed.关键词：naringin;metabolism;human intestinal microflora;naringenin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the molecular mechanisms of Jinlida on ameliorating insulin sensitivity in insulin resistant rats. Method: Fourty-eight SD rats were randomly divided into 2 groups: the control group (normal diet) and the high-fat-diet group (high-fat diet). After 6 weeks of high-fat diet, the insulin resistant model in rats was tested by the euglycemic hyperinsulinemic clamp. Then the high-fat-diet rats were randomly subdivided into 5 groups: the high-fat group (HF), the low-, middle- and high-dose Jinlida groups (0.75, 1.5,3.0 g·kg-1) and the metformin group (0.2 g·kg-1). After 8 weeks of treatment, hyperinsulin-euglycemic clamp and intraperitoneal glucose tolerance test were performed to evaluate the whole-body insulin sensitivity. Blood samples were collected and fasting blood glucose (FBG), fasting plasma insulin (FINS), glycosylated hemoglobin (HbA1c), total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) were tested. The mRNA expressions of insulin signaling pathway molecules such as insulin receptor (INSR), insulin receptor substrate-1 (IRS-1), phosphatidylinositol3 kinase (PI3K), protein kinase (AKT) and glucose transporter (GLUT2) were investigated by quantitative RT-PCR. The protein expressions of phosphorylation and total IRS-1, AKT were determined by Western blot. The phosphorylated Akt/total Akt (p-Akt/Akt) and phosphorylated IRS-1/total IRS-1(p-IRS-1/IRS-1) were evaluated. Result: Compared with the control group, glucose infusion rate (GIR) decreased, levels of FBG, FINS, HbA1c, TG, TC,LDL-C and VLDL-C increased, level of HDL-C decreased, mRNA expressions of INS, IRS-1, AKT and GLUT2 decreased in the HF group (P<0.05). Compared with the HF group, GIR decreased, levels of FBG, FINS, HbA1c, TG, TC and VLDL-C increased, mRNA expressions of INS, IRS-1, AKT and GLUT2 increased, the ratio of p-IRS-1/IRS-1 decreased, the ratio of p-AKT/AKT increased in the Ji Lida groups (P<0.05). However, there was no significant effect on the level of HDL-C and expression of PI3K in the Ji Lida groups. Conclusion: Jinlida could effectively reduce the insulin resistance and improve the glucose and lipid metabolic disorder in high-fat-diet rats. The possible molecular mechanism might be related to inhibiting the PI3K/AKT signaling pathway.关键词：Jinlida;liver;PI3K/AKT signal pathway;insulin resistance;high fat diet
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发布时间：2024-01-04 - 摘要：Objective: To study inhibitory effect of folate receptor active targeting docetaxel-loaded liposomes (FA-PDCT-L) on S180 cell transplanted tumor and its in vivo toxicity in mice. Method: A self synthesized amphiphilic copolymer,folate-poly (PEG-cyanoacrylate-co-cholesteryl cyanoacrylate) (FA-PEG-PCHL) was used as liposomal modifying material to form FA-PDCT-L.Confocal laser scanning microscopy was adopted to detect affinity between liposomes and MCF-7 cells.Subcutaneous transplanted model of S180 sarcoma was made.Tumor mice were randomized into 4 groups,such as docetaxel (DCT) injection (DCT-I),unmodified DCT-loaded liposomes (DCT-L),FA-PDCT-L and model group (saline),dosages were 10 mg·kg-1·d-1 through tail vein.Tumor weight and inhibition rate of tumor were detected at the end of experiments,tumor cell apoptosis was analyzed by TUNEL.Hepatic function and hematology assay for myelosuppression were evaluated. Result: FA-PDCT-L had better affinity to cells than the other two reference preparation.Compared with model group,weight of tumor in DCT-I,DCT-L and FA-PDCT-L were decreased,especially for FA-PDCT-L,its inhibitory rates was 79.03% with apoptosis index at (45.7±3.4)%.There was no significant difference of hepatic function and hematology assay between the FA-PDCT-L group and the blank group. Conclusion: FA-PDCT-L is internalized into cells by mediation of FA-PEG-PCHL with better anti-tumor activity and lower in vivo toxicity by compared with DCT-I.关键词：docetaxel;active targeting liposomes;anti-tumor activity;toxicity;coumarin 6
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of the compatibility of Epimedii Folium and Ligustri Lucidi Fructus decoction and their extract on retinoic acid-induced osteoporosis in rats. Method: Thirty-six male Wistar rats were randomly divided into 6 groups: the normal group, the model group, the combination of Epimedii Folium and Ligustri Lucidi Fructus group (9.5 g·kg-1), and three extract combination groups (Extract-L 50 mg·kg-1, Extract-M 100 mg·kg-1, and Extract-H 200 mg·kg-1) of 10 rats each. The osteoporosis model was induced in rats by intragastric administration of retinoic acid. The protein expressions of apoptosis factor B-cell lymphoma/Leukemia-2 (Bcl-2) and p53 of bone tissue were detected by immunohistochemical methods. The mRNA expressions of transforming growth factor beta-1 (TGF-β1), Smad2, Smad3 and Smad7 of bone tissue were detected by using real-time RT-PCR. Result: Compared with the normal group, the protein expression of Bcl-2 decreased significantly, p53 protein increased significantly, mRNA expressions of TGF-β1, Smad2, Smad3 decreased significantly, and Smad7 increased significantly in the model group (P<0.05, P<0.01). Compared with the model group, the expression levels of Bcl-2, TGF-β1, Smad2 and Smad3 increased, the expression levels of p53 and Smad7 decreased in all treatment groups (P<0.05, P<0.01). Conclusion: The mechanism of the compatibility decoction and their extracts against osteoporosis is related to maintaining the balance between apoptosis and anti-apoptotic, inhibiting of apoptosis of bone tissue, regulating and controlling the TGF-β/Smads signal transduction pathways.关键词：Epimedii Folium/Ligustri Lucidi Fructus;retinoic acid;osteoporosis
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发布时间：2024-01-04 - 摘要：Objective: To explore the influence of vitamin B1 (VB1) on the effect of Anshen Bunao syrup. Method: The experimental mice were randomly divided into 7 groups: the control group (distilled water), the high and low-dose Anshen Bunao syrup groups (10, 5 mL·kg-1), the high and low-dose Anshen Bunao syrup without containing VB1 groups (10, 5 mL·kg-1), the high and low-dose VB1 oral liquid groups (10, 5 mL·kg-1) of 10 mice each. The corresponding medicines were intragastrically administered to the mice according to body weight once daily for 10 days. The sedative effects of different groups were observed by the locomotor activity in mice; the hypnotic effects were observed by the sleeping time in mice with pentobarbital sodium; the RNA content of brain tissue was measured by orcinol method; the DNA content of brain tissue was determined through diphenylamine colorimetric method, and protein content of brain tissue was determined by lowry method. Result: Compared with the control group, the times of spontaneous motion (the indication of sedation) in mice significantly decreased in the high- and low-dose groups of Anshen Bunao syrup and Anshen Bunao syrup without containing VB1 (P<0.05). There were no significant differences for the times of spontaneous motion between VB1 oral liquid groups and the control group. The sleep time by pentobarbital sodium significantly increased in the high-dose Anshen Bunao syrup group (P<0.05). There was an increasing tendency for the sleep time by pentobarbital sodium in Anshen Bunao syrup without containing VB1 groups. There were no significant differences among other groups. The DNA content of brain increased significantly in all groups except the low-dose Anshen Bunao syrup group. The protein content increased significantly in the high-dose Anshen Bunao syrup without containing VB1 and high-, low-dose VB1 oral liquid groups (P<0.01). The RNA content in the brain had no significant difference between any two groups. Conclusion: The sedative-hypnotic effect of Anshen Bunao syrup without containing VB1 is similar to that of Anshen Bunao syrup. Simultaneously, our results prove that the different processes samples could promote DNA and protein synthesis of brain tissues, but they have no influence on RNA content in mice.关键词：Anshen Bunao syrup;Anshen Bunao syrup without containing VB1;VB1 oral liquid;sedative;hypnotic
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发布时间：2024-01-04 - 摘要：Objective: To study the adjustment mechanism of tea pigments (TP) on isolated toad myocardial contraction. Method: Toads were randomly assigned to the control groups and the experimental groups, the control groups were then divided into the normal Ringer solution group, the negative group, the positive group and the drug group. The normal Ringer solution group received Ringer solution only, the negative groups received low calcium Ringer solution following Ringer solution, the positive group received high calcium Ringer solution following Ringer solution and the drug group received TP, verapamil, atropine or isopropyl epinephrine solution, respectively, following Ringer solution. The effects on specimens of isolated toad hearts were observed. The experimental groups were divided into the verapamil group, the atropine group and the isopropyl adrenaline. All drug groups in the experimental groups included the low calcium and high calcium groups, and the low calcium and high calcium group was then divided into two groups as before or after TP-adding of 8 toads each. The perfusion specimens of isolated toad heart were prepared by Si's method. The specimens were perfused by verapamil (7.27 mg·L-1), atropine (10 mg·L-1) and isoproterenol (10 mg·L-1) before or after TP adding (200 mg·L-1). The toad myocardial contraction curves were recorded by using BL-420F biological function in vitro experimental system, and the myocardial contractility was analyzed statistically. Result: Myocardial contractility increased when perfusing low calcium and inhibited when perfusing high calcium (P<0.05), and it could be blocked partially by verapamil and stimulated by isoprenaline (P<0.01). It had both cooperation and antagonism on atropine (P<0.05). Conclusion: The protective effect of TP on isolated toad heart may be associated with calcium channels, M receptor and β receptor.关键词：tea pigment;isolated toad heart;myocardial contraction force;verapamil;atropine;isoproterenol adrenaline
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发布时间：2024-01-04 - 摘要：Objective: To explore TCM syndromes in abnormal glucose metabolism syndrome(MS) rats based on syndrome detecting from recipe used. Method: After the establishment of the MS rat model of abnormal,according to sugar of blood level, rats were divided into MS group, group A, group B, group C. Liujunzi Tang(A,2.5 g·kg-1),Shenqi Dihuang Tang(B, 25 g·kg-1), Shenqi Wan(C, 0.5 g·kg-1) were given.At 12 and 15 weeks, the weight, blood pressure, fasting blood glucose(FBG), triglycerides(TG), high density lipoprotein(HDL-C) and fasting insulin (FIN) levels were measured. Result: Compared with the model group, in A,B,C group, body weight, blood pressure, FBG, TG, HDL-C had no significant forward difference, in A, B, C group FIN and insulin resistance index(HOMA-IR) was significantly lowered(P<0.01). Conclusion: Abnormal glucose metabolism with metabolic syndrome in rats after intervention significantly improved insulin levels, according to the measured in square card method, shows that the spleen with phlegm, Yin and Yang dificiency syndrome in two, two Qi and Yin dificiency syndrome can exist at the same time, which is a deficiency syndrome type.关键词：metabolic syndrome;merger abnormal glucose metabolism;TCM syndromes
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of flaxseed on the content of testosterone, correlated hormones and anti-fatigue ability of rats after exercise. Method: By using the model of high-intensity endurance training, 55 6-week-old male Wistar rats were randomly divided into 5 groups, with 10 in each group (5 rats which did not meet the requirement were removed):control group (C group), motion group (M group), exercise+ig low-dose flaxseed group (FMⅠ group), exercise+ig middle-dose flaxseed group (FMⅡ group), and exercise+ig high-dose flaxseed group (FMⅢ group). Gavage was performed using professional device once a day. The rats in flaxseed groups were gavaged with 0.75,1.5,4.5 g·kg-1 with ig volume of 5 mL·kg-1. The rats in C and M groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: Body weight of the rats in M groups was lower than that in C group (P<0.05), and in all doses of flaxseed groups was higher than in M group (P<0.05) which did not show any differences between flaxseed groups. Swimming time in all doses of flaxseed groups was longer than in M group (P<0.01), and there were dose-response. Serum testosterone in M group was lower than C group (P<0.01), in the same time, serum testosterone was higher in all doses of flaxseed groups than M group [FMⅠ(4.83±1.15) nmol·L-1,FMⅡ (4.93±1.13) nmol·L-1, FMⅢ (5.11±1.12) nmol·L-1, P<0.01]. The serum corticosterone levels in each group showed no significant differences. Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. There were no differences in luteinizing hormone and follicle-stimulating hormone between C group and M group. But luteinizing hormone in all doses of flaxseed groups was higher than in M group (P<0.05). In mean time, follicle-stimulating hormone in all doses of flaxseed groups was higher than in M group (P<0.05). There were no differences in both luteinizing hormone and follicle-stimulating hormone between groups in all doses of flaxseed groups. Conclusion: The supplement of flaxseed can alleviate the hypothalamus-pituitary-gonad axis function disorder caused by exercise through multiple targets and multiple ways. Finally, flaxseed can prevent the decrease of serum testosterone after high-intensity exercise and improve anti-fatigue ability.关键词：flaxseed;testosterone;corticosterone;luteinizing hormone;follicle-stimulating hormone;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: To investigate antiviral activity of Jinqiao Reduqing granules and Jinqiao Reduqing spray drying powder, and compare the difference of antiviral activity between them in vitro and in vivo. Method: In vitro experiment, the 3 -(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)colorimetric method was applied to determine the 50% inhibitory concentration (IC50) against influenza virus FM1 of Jinqiao Reduqing granules and spray drying powder by different administration ways (drug was used directly on the virus or virus-infected cells). Therapy indexes (TI) of them were also calculated. The mice were randomly divided into control group, Ribavirin(0.07 g·kg-1),Jinqiao Reduqing granules(11.7 g·kg-1),Jinqiao Reduqing spray drying powder(5.85,11.7,23.4 g·kg-1). In vivo experiment, the mice were infected with influenza virus FM1 through nasal inhalation,the mice were intragastric administrated after infection virus. The life-extension rate, lung index of infected mice were observed to investigate the protective function of Jinqiao Reduqing spray drying powder and Jinqiao Reduqing granules against influenza virus. Result: For two different administration ways, the IC50 of Jinqiao Reduqing spray drying powder was 0.77, 0.80 g·L-1, and the TI were 11.78, 11.34 respectively. The IC50 of Jinqiao Reduqing granules were 3.70, 3.60 g·L-1, and the TI were 6.37,6.55 respectively. At the dose of 23.4 g·kg-1 for Jinqiao Reduqing spray drying powder, its life-extension rate was 60.81%, and the lung index inhibition rate was 13.18%.Compared with the virus control group, the difference were statistically significant(P<0.05). Conclusion: Jinqiao Reduqing spray drying powder and Jinqiao Reduqing granules have anti-influenza virus activity. But at the same dose, the anti-influenza virus FM1 function of Jinqiao Reduqing spray drying powder was more powerful than that of Jinqiao Reduqing granules in vitro.关键词：Jinqiao Reduqing granules;Jinqiao Reduqing spray drying powder;influenza virus;antiviral activity
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of Baogan granules on acute alcoholic-induced liver injury in mice. Method: The experimental mice were randomly divided into the normal group, the model group, the Hugan tablet group (0.7 g·kg·d-1), the Like group (1.18 g·kg-1·d-1), the high-, medium-, and low-dose Baogan granules groups (12.9, 6.45, 3.225 g·kg-1·d-1). The mice in the normal and model groups received an equal volume of saline. All mice received 30-day oral administration of the corresponding medicines. The acute liver injury model was induced in mice by feeding 50% alcohol at 0.012 mL·g-1 1 h later after the last administration. The serum levels of alanine aminotransferase (ALT), aspartic transaminase (AST), total bilirubin (TBIL), triglyceride (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were detected. Histopathological changes of liver tissue were observed by using the HE staining. Result: Compared with the model control group, the levels of ALT, AST, TBIL, TG and LDL increased, the level of HDL decreased in the model group (P<0.05, P<0.01). Compared with model group, the levels of ALT, AST, TBIL, TG and LDL decreased, the level of HDL increased in the high-, medium-, and low-dose Baogan granules groups (P<0.05, P<0.01). Pathological results showed that the liver cell morphology, structure, edema and inflammatory infiltration had different degrees of improvement in the Baogan granules groups. Conclusion: Baogan granules could obviously inhibit the serum levels of ALT, AST, TG and LDL; promote the HDL generation in mice. However, its effect on TBI is unclear. Moreover, it could protect the alcoholic-induced liver injury by improving liver edema and inflammatory cell infiltration.关键词：Baogan granules;acute alcoholic-induced liver injury;liver function;lipid metabolism;pharmacodynamics
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发布时间：2024-01-04 -
Effect of Changkang Formula on SP and VIP in Rats with Diarrhea-predominant Irritable Bowel Syndrome
摘要：Objective: To study the effects of Changkang formula (CF) on plasma P substance (SP) and colon vasoactive intestinal peptide (VIP) in rats with diarrhea-predominant irritable bowel syndrome (D-IBS), and to explore its possible mechanism on D-IBS. Method: Sixty Sprague-Dawley rats were randomly divided into 6 groups: the normal control group (normal saline), model group (normal saline), the maleate trimebutine group (15 mg·kg-1), the low-, middle and high-dose CF groups (1.1, 2.2, 4.4 g·kg-1). The D-IBS model was induced in rats by senna-free drinking and stress-restrainting for 4 weeks. The corresponding medicines were intragastrically administered to rats once daily for 3 weeks. The abdominal withdrawal reflex (AWR) was scored. The levels of SP in serum and VIP in tissue were detected by using the ELISA assay. Result: Compared with the normal group, AWR score and plasma SP level increased, colon VIP level decreased in the model group (P<0.05, P<0.01). Compared with the model group, AWR score and plasma SP level decreased, colon VIP level increased in the low-, middle and high-dose CF groups (P<0.05, P<0.01). Conclusion: CF had a therapeutic effect on D-IBS by improving visceral hypersensitivity, reducing the level of plasma SP, and elevating the level of colon VIP in rats.关键词：Changkang formula;diarrhea-predominant irritable bowel syndrome;plasma substance P;vasoactive intestinal peptide- 3
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发布时间：2024-01-04 - 摘要：Objective: To observe the direct protective effect of Cidan injection on isolated rat heart. Method: SD rats, half male and half female, were randomly divided into 4 groups: the control group (physiological saline, 10 mL·L-1), the Acanthopanax senticosus injection group (10 mL·L-1), the Salvia miltiorrhiza injection group (10 mL·L-1) and the Cidan injection group (8 mL·L-1) of 8 rats each. The Langendorff heart perfusion in vitro model of 8 channels physiological signal recording system was used in this study. The change of left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), left ventricular pressure (LVDP=LVSP-LVEDP), left ventricular pressure maximum rising rate (+dp/dtmax), left ventricular pressure maximum decline rate (-dp/dtmax) and heart rate (HR) were tested. The increasing amount of each index between Cidan injection group and Acanthopanax senticosus injection group, Salvia miltiorrhiza injection group was compared, respectively. The Cidan injection groups were then divided into 5 dose nutrition perfusate groups (1,2,4,8,16 mL·L-1), and the dose effect relationship was investigated. Result: Compared with the control group, LVSP, LVDP, +dp/dtmax increased, HR, LVEDP and -dp/dtmax decreased in the Cidan injection group. There were significant differences for the increasing amount of each index as compared with the Acanthopanax injection and Salvia miltiorrhiza injection groups (P<0.05, P<0.01). Cidan injection showed a certain dose effect relationship within the dose of 1-8 mL·L-1, and it had the most significant efficacy at the dose of 8 mL·L-1. Conclusion: Cidan injection could slow the HR, improve cardiac systolic and diastolic function, and the effect is better than Acanthopanax senticosus injection and salvia miltiorrhiza injection. Cidan injection shows a dose-dependent effect in a certain range of dose.关键词：Cidan injection;Langendorff isolated heart perfusion;heart function parameters;dose effect relationship
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发布时间：2024-01-04 - 摘要：Objective: To study the acute toxicity of different processed products of Aconiti Lateralis Radix. Method: Fifty SPF Kunming mice, half male and half female, were randomly divided into the normal group (normal saline), the Heishunpian group, the high-temperature group, the high-pressure group and the microwave group of 10 mice each. The maximum amounts of the corresponding medicines were intragastrically administered to mice and the acute toxicity was observed. Result: Compared with the normal group, cardiac index increased in the high-pressure and microwave groups, hepatic and spleen index decreased in the high-temperature and microwave groups. There was statistical significance for the difference (P<0.05). No obvious changes for body weight and other visceral indexes were found. The pathology results showed that there was serious toxicity in heart, liver and kidney of mice. Conclusion: The maximum amount of three processed products of Aconiti Lateralis Radix was 48 g·kg-1 for mice, which was 192 times higher than the clinical dosage in humans. Three new processing products of Aconiti Lateralis Radix would be safe in clinical application.关键词：new processing technology;Aconiti Lateralis Radix;decoction;acute toxicity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects and mechanism of Chaihu Shugan powder (CHSGS) on hepatic fibrosis in rats. Method: Sixty Wistar rats were randomly divided into 6 groups:normol group,model group, Chaihu Shugan powder high-dose,mediate-dose,low-dose, and prevention group, except the normol group,all the other groups were injected with pig's serum without inactivation into abdominal cavity, twice per week and 0.5 mL each time, persisting for 10 weeks. The prevention groups were injected the stomach with the medicines(Chaihu Shugan powder 6.3 g·kg-1,10 weeks)at the same time when models began to be made, the other treatment groups were dealed with just as the prevention groups 6 weeks later persisting for 4 weeks. Then rats were randomly taken for decollation in all 6 groups at the end of 10th week. Chaihu Shugan powder high-dose group 12.6 g·kg-1,mediate-dose group 6.3 g·kg-1,low-dose group 3.15 g·kg-1. RT-PCR assay were used to detect the expression of transforming growth factor-β1(TGF-β1),Smad2,Smad3,Smad4 and Smad7mRNA. The expression of TGF-β1,p-Smad 2/3 and Smad7 protein in hepatic fibrosis tissues was detected by immunohistochemical techniques. Result: The expression of TGF-β1,Smad2,Smad3 mRNA and the expression of TGF-β1,p-Smad 2/3 protein were markedly higher, while the expression of Smad7 gene were significantly lower in the model group than those in normal group(P<0.01). Compared with the model group,the expression of TGF-β1 and Smad3 mRNA were markedly lower, but the expression of Smad7 gene were significantly higher, the expression of p-Smad 2/3 and TGF-β1 protein were markedly lower(P<0.05, P<0.01)in Chaihu Shugan powder mediate-dose group. Conclusion: Chaihu Shugan powder have a positive action on hepatic fibrosis model rats.The mechanism of its effect is related with its inhibiting the expression of TGF-β1, Smad3 genes,and p-Smad 2/3 protein,and enhancing the expression of Smad7 gene in rats liver.关键词：Chaihu Shugan powder;hepatic fibrosis;transforming growth factor-β1;Smad protein
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protected effects and mechanism of ursolic acid (UA) against focal cerebral ischemic-reperfusion injury in rats. Method: Totally 120 Sprague-Dawley (SD) rats were randomly divided into six groups as following:sham operation group, ischemic-reperfusion model group, UA 20, 40, 80, 120 mg·kg-1treated groups,20 expremental animals every group, respectively. The model of focal cerebral ischemic reperfusion was established with Zea-Longa occluding suture in rats. Drugs were given immediately by tail injection when the suture was inserted. And 6 h later, the neurological deficits, ratio of infarct volume, water content of the brain were evaluated. Total antioxidative capacity(T-AOC) and the content of creatine kinase (CK), lactate dehydrogenase(LDH), malondialdehyde(MDA) in serum were determined. The activity of superoxide dismutase(SOD),glutathione peroxidase,(GSH-Px),catalase(CAT) and the content of MDA in brain tissue were determined, and the histopathological changes were observed by haematoxylin and eosin(HE) staining. Result: Compared with sham operation group, the neurological scores of focal cerebral ischemia-reperfusion model group was significantly decreased (P<0.01);the content of CK, LDH, MDA in serum were significantly increased (P<0.01), and the level of T-AOC was significantly decreased (P<0.01);the ratio of infarct volume and water content of the brain were significantly increased (P<0.01);the activity of SOD,GSH-Px,CAT in brain tissue were significantly decreased (P<0.01), the content of MDA was significantly increased (P<0.01);and the histopathological changes of brain tissue was appeared. Compared with focal cerebral ischemia-reperfusion model group, the neurological scores of UA (40, 80, 120 mg·kg-1) treated groups was significantly decreased (P<0.05, P<0.01), and the contents of CK, LDH, MDA in serum were also significantly decreased (P<0.05, P<0.01), while T-AOC was significantly increased (P<0.05, P<0.01). The ratio of infarct volume and brain water content of UA (80, 120 mg·kg-1) treated groups were significantly decreased (P<0.05, P<0.01), and the activity of SOD,GSH-Px,GST in brain tissue of UA (80, 120 mg·kg-1) treated groups was significantly increased (P<0.05, P<0.01), and the histopathological changes of UA treated groups were significantly improved compared with ischemia-reperfusion model group. Conclusion: UA can protect focal cerebral ischemic-reperfusion injury in rats, which may be related with its pharmacological effects of enhanceing the activity of antioxidant enzymes and free radical scavenging capacity.关键词：ursolic acid;cerebral ischemic-reperfusion;protect;mechanism
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects of Fangji Huangqi Tang(FJHQT) on ischemia-reperfusion(IR) cerebral injury in rats and provide experimental evidence for clinical application of FJHQT on cerebral ischemia. Method: The ischemia-reperfusion model was reproduced by cerebral middle artery occulation for one day. The model rats were randomly allotted into five groups as following:FJHQT 20, 40 mg·kg-1, model, sham and positive drug group nimodipine in accordance with the neurobehavioral scores qualified. After being administered orally FJHQT (crude drug,1,2 g·kg-1) or nimodipine (2 mg·kg-1) for continuous 7 days, the cerebral infarct size was observed, and cerebral Na+-K+-adenosine triphosphate(Na+-K+-ATP), peduced glutathione(GSH), catalase(CAT) activity and inflammatory cytokines interleukin-6(IL-6), interleukin-1β(IL-1β), and tumor necrosis factor α(TNF-α) levels were measured. terminal dUTP nick end labaling(TUNEL) assay was used to determine apoptotic cells in brain tissue. In addition, immunohistochemistry and Western blot analysis were used to determine the expression of Caspase-3. Result: Compared with the sham group, the activities of Na+-K+-ATP, GSH, CAT were decreased in brain tissue of model rats (P<0.01). while inflammatory cytokines IL-6, IL-1β and TNF-α were increased (P<0.01); furthermore, apoptosis level was significantly increased, and the expression of apoptosis protein Caspase-3 also was significantly increased (P<0.01). Compared with the model group, FJHQT could increase Na+-K+-ATP, GSH, CAT activity(P<0.05,P<0.01), and reduce IL-6, IL-1β and TNF-α levels (P<0.05,P<0.01). FJHQT significantly reduced the degree of apoptosis in brain tissue, as well as Caspase-3 expression (P<0.05,P<0.01). Conclusion: FJHQT can significantly protect against the cerebral ischemia, involving in reduce infarction area of ischemic brain, decrease apoptosis, oxidative damage, inflammation.关键词：cerebral ischemia-reperfusion;Fangji Huangqi tang;oxidative damage;apoptosis;inflammation
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发布时间：2024-01-04 - 摘要：Objective: This study aimed to investigate the impact of Tangshenning on the levels of hepatocyte growth factor (HGF),cystatin C(CysC) and transforming growth factor-β1(TGF-β1) in patients with two deficiency of Qi and Yin and blood stasis-typed early diabetic nephropathy, to provide reference for clinical diagnosis and treatment. Method: According to random number table method, 90 cases of Qi and Yin and blood stasis-typed early diabetic nephropathy patients were randomly divided into control group (n=45) and observation group (n=45). The control group was given Luodingxin treatment, on the basis of the control group, the observation group was treated with Chinese herbal medicinal Tangshenning. The curative effects, TGF-β1, urinary albumin excretion rate (UAER), homocysteine (Hcy), CysC, HGF, serum creatinine (SCr), and creatinine clearance (CCr) were observed. Result: The total effective rate in observation group (93.33%) was significantly higher than control group (75.56%), and there were significant differences (P<0.05);levels of serum UAER, Hcy, CysC after treatment in two groups were significantly lower than before treatment, with significant difference (P<0.05);serum UAER, Hcy, CysC after treatment in observation group were significantly lower than those in control group, with significant difference (P<0.05);TGF-β1 in blood and urine after treatment were significantly lower than before treatment, with significant differences (P<0.05);level of serum HGF was higher than before treatment, with significant differences (P<0.05);TGF-β1 in blood and urine and CCr after treatment in observation group were significantly lower than the control group, qith significant differences (P<0.05);level of serum HGF after treatment in observation group was significantly higher than in control group, and thedifference was significant (P<0.05). Conclusion: The effect of Tangshenning combined with western medicine on the treatment of Qi and Yin and blood stasis-typed early diabetic nephropathy patients is significantly evident, it can significantly improve HGF to protect kidney tissue, and reduce the content of CysC to improve glomerular permeability, as well as reduce the content of TGF-β1 to inhibit cell fibrosis.关键词：Tangshenning;Qi Yin deficiency of clip stasis type;early diabetic nephropathy;hepatocyte growth factor;cystain C;transorming growth factor-β1
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Ailuo Kechuanning(ALKC) on interleukin-4(IL-4),interleukin-8(IL-8),tumor necrosis factor alpha(TNF-α) and pulmonary function in elder chronic obstructive pulmonary disease(COPD) patients with acute exacerbation and syndrome of damp-phelgm stagnating the lung. Method: Fifty cases of elder COPD patients with acute exacerbation and syndrome of damp-phelgm stagnating the lung were selected by the muti-center,randomized,single-blind,placebo-controlled clinical trails methods.Fifty cases were randomly divided into two groups, ALKC group (n=25) and placebo-controlled group(PC group, n=25). On the base of the western drug treatment, ALKC and placebo was used in the control group. Every group was observed in terms of improvement of the symptoms, pulmonary functions, cells factors such as IL-4,IL-8,TNF-α. Result: The differences of demogra-phic, pulmonary functions, cell factors like IL-4,IL-8,TNF-α between two groups had no significant statistical differences prior to our trails. Compared with the PC group, the total effective rate in forced expiratory volume in 1 second(FEV1),FEV1/forced vital capacity(FVC),IL-8(0.42±0.10) μg·L-1 vs (0.48±0.12) μg·L-1,(P<0.05),IL-4(0.28±0.09)μg·L-1 vs (0.32±0.06)μg·L-1,(P<0.05),TNF-α(0.87±0.40)μg·L-1 vs (1.28±0.25)μg·L-1,(P<0.05)in ALKC group had significant statistical differences (P<0.05, P<0.01), had no significant statistical differences in FVC. Conclusion: ALKC has the improvements action on clinical symptoms, pulmon-ary function and the control of inflammation in elder COPD patients with acute exacerbation and syndrome of damp-phelgm stagnating the lung.关键词：chronic obstructive pulmonary disease;damp-phelgm stagnating the lung;interleukin-4;interleukin-8;tumor necrosis factor alpha;pulmonary function
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to observe the clinical therapeutic effect and the change of life quality of Huatan Xifeng method in patients with epilepsy the method. Method: The sixty-one patients with epilesy who met the inclusion criteria were randomized divided into treatment group and control group. Both groups were treated by the routine antiepileptic drugs. The patients in treatment group received traditional Chinese medicine (TCM) soup according to the method of Huatan Xifeng, additionally. The course of treatment in both group lasted for 3 months. Clinical therapeutic effect and the change of the score of the quality of life in epilepsy-31(QOLIE-31) were compared. Result: The clinical efficacy in treatment group was 0 case as clinical recovery, 4 cases as markedly effective, 21 cases as effective, and 5 cases as invalid. In control group, the clinical efficacy was 0 case as clinical recovery, 0 case as markedly effective, 14 cases as effectiv, 17 cases as invalid.The total effective rate in treatment group was 83.33%, while in control group it was 45.16%, with statistically significant difference (P<0.01).After treatment, the scores of QOLIE-31 in both group were higher than before treatment. The difference was statistically significant (P<0.01). But the difference between two groups was not statistically significant. Further analysis of each factors:in treatment group, the difference in six aspects including spiritual state, social function, cognitive function, the effect of drugs, worry to seizure and feeling of health were statistically significant (P<0.05). The difference in emotional state was not statistically significant.In control group, the difference in five aspects including spiritual state, social function, cognitive function, the effect of drugs, worry to seizure were statistically significant (P<0.05). The difference in emotional state and feeling of health was not statistically significant. In the third month, the scores of QOLIE-3 in treatment group in three aspects spiritual state, worry to seizure and feeling of health were higher than in control group. The difference was statistically significant (P<0.05). Conclusion: The TCM soup according to the method of Huatan Xifeng with routine antiepileptic drugs can ameliorates the state of the epilepsy patient, such as spirit, physical strength and sleep, and improve the quality of life in epilepsy patients.关键词：epilepsy;quality of life;integrated Chinese and western medicine
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发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to observe the curative effect of the Dasangju mixture combined with azithromycin on mycoplasma pneumoniae (MPP) in children and investigate the bi-directional control mechanism of inflammatory response. Method: One hundred patients with MPP were randomly divided into control group and observation group with 50 patients in each group by random number table. Patients in control group took 10 mg·kg-1 azithromycin suspension on the first day as well as 5 mg·kg-1 orally daily for 4 days and stopped for 2 days, which was a course of treatment. Based on the control group, patients in observation group was added 10 mL Dasangju mixture at a time and three times a day in the children aged above 3 years old, while 5 mL at a time and three times a day in those aged younger than 3 years old. Both groups were treated for 21 days, with 7 days as a course of treatment. Then, the symptoms and signs before and after treatment were recorded and the results were evaluated by efficacy evaluation in Chinese medicine. Moreover, the serum concentrations of myeoplasma pneumoniae-immune gloulin M(MP-IgM), interleukin-6(IL-6), and IL-10 were determined before and after medication on the 1st day and the 21st day. Result: The duration of observation group on fever, cough and lung rale were obviously improved than the control group(P<0.05). The total effective rate in observation group was 88.00% while in the control group it was 70.00%, and there was statistical significance(P<0.05). After treatments, both the serum concentrations and the differences between before and after treatment of interleukin-6 and interleukin-10 in observation group were more significantly reduced than in the control group(P<0.05). Conclusion: Dasangju mixture combined with azithromycin can not only improve the clinical symptoms and signs, but also shorten the duration with significant effect. Meanwhile, Dasangju mixture can significantly reduce the concentrations of IL-6 and IL-10, which may be related to bi-directional control mechanism of inflammatory response.关键词：Dasangju mixture;inflammatory response;mycoplasma pneumoniae
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to investigate the clinical effect of Danhong injection with atorvastatin on endothelial function and inflammation factors in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI) surgery, and provide clinical basis for ACS therapy. Method: From 2012/5 to 2014/5, we collected 125 ACS cases treated with PCI in our hospital and divided them into 2 groups randomly, with 62 cases in control group and 63 cases in observation group. Besides conventional treatment, the control group was treated with atorvastatin and the observation group was treated with danhong injection combined with atorvastatin. Before and after treatment, the indexes of endothelial function including endothelin-1 (ET-1), nitrogen monoxide (NO), von willebrand factor (vWF) and flow-mediated dilation (FMD), inflammation factors including tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and C reactive protein (CRP), as well as complications were observed and compared. Result: Compared with those before treatment, the indexes of endothelial function including ET-1, NO, vWF and FMD were all improved after treatment. However, these indexes decreased more significantly in the observation group than in the control group, and all differences were statistically significant (P<0.05). The inflammation factors including TNF-α, IL-1 and CRP were all decreased after treatment in both groups. However, those decreased more significantly in the observation group than in the control group, with all differences statistically significant (P<0.05). Complications in the observation group was significantly lower than in the control group(7.9% VS 32.3%, P<0.05). Conclusion: Combination treatment of danhong injection and atorvastatin for ACS therapy can improve endothelial function and decrease inflammation factors and is worthy of study and further application in clinical treatment.关键词：Danhong injection;atorvastatin;acute coronary syndrome;C reactive protein
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发布时间：2024-01-04 - 摘要：Objective: The study aimed to observe the clinical efficacy and safety of Shenling Baizhu powder on patients with hyperlipidemia. Method: In this study, 136 patients with hyperlipidemia were randomly divided into the treatment group(referred to as Shenling Baizhu powder treatment) and the control group(referred to as the western medicine simvastatin treatment)with 68 patients in each group. Each group was treated for 8 weeks, and the clinical effect, lipid changes,blood rheology changes and side-effects before and after treatment were observed. Result: ① The total effective rate of the treatment group was 86.8%,while it was 83.8% in the control group. With no significant difference. ② The total effective rate of traditional Chinese medicine(TCM)syndrome in treatment group was 89.7%, while it was 54.4% in control group, with significant difference between two groups (P<0.01). ③There were significant change in the total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C)between the two groups after treatment (P<0.01,P<0.05). The effects of reducing TG in the treatment group[the treatment group was (1.86± 0.51) mol·L-1,and the control group was(2.54±0.69) mmol·L-1]and elevating HDL-C[The treatment group was (1.74±0.52),and the control group was (1.19±0.40) mmol·L-1]were better than those in control group after treatment (P<0.05).④ In the two groups, the whole blood high shear viscosity, whole blood low shear viscosity, plasma viscosity and erythrocyte deposited were all had significantly lower than before treatment (P<0.05), but with no significant difference between the two groups.⑤No side-effects were observed in treatment group. Conclusion: The total effective rate of Shenling Baizhu powder is equivalent to simvastatin in treating hyperlipidemia, but better than control group in reducing TG and elevating HDL-C is, which can alleviate the syndromes of patients, improve the blood rheology, with no side-effects.关键词：Shenling Baizhu powder;hyperlipidemia;TCM therapy
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to observe the efficacy and effect of acupuncture in combination with gastrodin on the serum level of endothelin-1(ET-1) and calcitonin gene-related peptide(CGRP) in the treatment of cervical vertigo (CV) and provide clinical reference for the CV treatment. Method: One hundred and fourty cases of cervical vertigo meeting the inclusion criteria were randomly divided into two groups equally. The control group was treated with acupuncture, and the observation group was treated with acupuncture and gastrodin. Clinical effects,vertigo dizziness handicap inventory(DHI) score, transcranial Doppler (TCD) value, serum ET-1 and CGRP were observed and compared. Result: The observation group had a therapeutic efficiency ratio of 97.1%, which was statistically higher than that of 74.3% in the control group (P<0.05). After the treatment, the DHI score in the observation group was decreased more than in the control group. As to TCD value valueof left vertebral artery (LVA), right vertebral artery (RVA) and basal artery (BA) in the observation group statistically increased more than those in the control group (all P<0.05). The serum level of ET-1 was decreased more while the level of CGRP was increased more in the observation group than in the control goup (P<0.05). Conclusion: The curative efficacy of acupuncture in combination with gastrodin in treating patients with cervical vertigo was reliable, which could significantly decrease the ET-1 level and increase the CGRP level.关键词：acupuncture;gastrodin;cervical vertigo;endothelin-1;calcitonin gene-related peptide
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Tongbi capsule combined with western medicine in the treatment of elderly patients with coronary heart disease and angina pectoris and guide the clinical application. Method: One hundred and eighty cases of angina patients were randomly divided into the study group and the control group with 90 cases in each. Control group received conventional western medicine treatment. Study group received Tongbi capsules 3 capsules each time, 3 times daily based on the control group, 8 weeks for 1 course. The clinical efficacies of two groups were compared. Result: There were 4 cases in study group and 3 cases in control group lost. The total efficiency in study group was 88.4%,significantly higher than that in control group (75.9%, P<0.05). Angina attack frequency, duration, angina pectoris integral, angina relief time after sublingual nitroglycerin, dosage of nitroglycerin in both groups decreased significantly while those in study group were significantly lower than that in control group. STsegment depression in lead number, T wave flattening the number of leads, T wave inversion number of leads in both groups decreased significantly while those in study group were significantly lower than that in control group. High sensitive C-reactive protein(Hs-CRP),homocysteine junction(HCY),interleukin-6(IL-6),matrix metallopeptidase 9(MMP-9) and myeloperoxidase(MPO)in both groups decreased significantly while those in study group were significantly lower than that in control group. Conclusion: Tongbi capsule combined with conventional western medicine can significantly improve the clinical symptoms in patients with coronary heart disease and angina pectoris, reducing the pathogenesis of angina pectoris frequencies and shorten the onset time, improving the abnormal ECG, and has obviously antioxidant and anti-inflammatory effects, also with long-term clinical benefit good, we recommend it a reasonably use in clinical.关键词：coronary heart disease;angina pectoris;integrated tcm-wm;Tongbi capsule
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发布时间：2024-01-04 - 摘要：Objective: To discuss the adjuvant effect of Hushen Kangfu decoction for assistance of hormone in treating recurrent pediatric nephrotic syndrome (PNS) disease of splenasthenic hygrosis type, and to investigate its influence on serum inflammatory factors and high blood coagulation state. Method: Ninety-six patients were randomly divided into the observation group (48 cases) and the control group (48 cases) via a digital method. Patients in the control group received hormone therapy, and patients in the observation group added Hushen Kanghu decoction. All patients in both two groups received 4 months of treatment. Levels of 24 h urine protein, total protein (TP), albumin (ALB), total cholesterol (TC), triglycerides (TG), fibrinogen (FIB), D-dimer(D-D), platelet aggregation rate (MPAR), tumor necrosis factor-α (TNF-α), hypersensitive C-reactive protein (hs-CRP) and interleukin 6 (IL-6) were detected before and after therapy. Besides, safety indicators of heart, liver and kidney were monitored. Result: The clinical curative effect in the observation group was superior to that in the control group (P<0.05). After treatment, levels of 24 h urine protein, TG and TC in the observation group were lower (P<0.01), while standards of TP and ALB were higher (P<0.01) than those in the control group. Standard of FIB, D-D and MPAR in the observation group were inferior to those in the control group after therapy (P<0.01). Besides, levels of TNF-α, hs-CRP and IL-6 in the observation group were lower than those in the control group (P<0.01). Conclusion: The clinical curative effect of hormone assisted with Hushen Kangfu decoction was superior to using Western therapy alone in treating recurrent PNS children. Its mechanism may be related to reducing inflammation, regulating lipid metabolism, enhancing blood high condensation state.关键词：nephrotic syndrome;splenasthenic hygrosis type;Hushen Kangfu decoction;inflammatory factor;hypercoagulative state
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy and action mechanism of Bushen Pinggan decoction for oral and bath foot combined conventional Western medicine in treating refractory hypertension of overabundant liver-fire syndrome. Method: Ninety-three patients were randomly divided into the control group (46 cases) and the observation group (47 cases) by a random number table. Patients in the control group received 50 mg Losartan Potassium tablets once daily, 10 mg Nifedipine tablets once daily and 25 mg Hydrochlorothiazide tablets twice daily based on the non-drug intervention. On the basis of the control group, patients in the observation group added Bushen Pinggan decoction for oral and bath foot. All patients in both two groups received 8 weeks of treatment. Blood pressure levels were measured before and after treatment, blood pressure success rate was recorded. Levels of nitric oxide (NO), endothelin 1 (ET-1), serum inhibition (Cys C) and β2 microglobulin (β2-MG) were detected. Result: Systolic blood pressure (SBP) and diastolic blood pressure (DBP) levels in the observation group were lower than those in the control group (P<0.01). The blood pressure control efficacy in the observation group was better than that in the control group (P<0.05). Blood pressure success rate was 41.3% in the control group, which was lower than that of 65.9% in the observation group (P<0.05). The CysC and β2-MG standards in the observation group were lower than those in the control group (P<0.01). After therapy, the ET-1 level in two groups declined, while the result was more obvious in the observation group (P<0.01). However, the NO in both groups went up, while the result was more obvious in the observation group (P<0.01). Conclusion: Based on the conventional Western medicine treatment, Bushen Pinggan decoction for oral and bath foot could control the blood pressure level, improve blood pressure success rate in refractory hypertension patients. Besides, it has a certain protective effect on early renal damage of hypertension. The mechanism may be related to regulating endothelial function.关键词：refractory hypertension;Bushen Pinggan decoction;foot bath with Chinese medicine;renal damage;endothelial function
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Bushen Huoxue Mingnu decoction in treating non-propagative diabetic retinopathy (NPDR) with the deficiency of liver and kidney combined collaterals stasis resistance of tradition Chinese medical (TCM) syndrome, and to investigate its influence on vascular endothelial cell growth factor (VEGF) and tumor necrosis factor-α (TNF-α). Method: Ninety-seven patients were randomly divided into the control group (47 cases) and the observation group (50 cases) by a random number table. Patients in the control group received 80 mg valsartan capsules once daily for controlling the blood glucose, 40 mg simvastatin tablets once nightly, 100 mg aspirin enteric-coated tablets once daily, and 0.5 g calcium dobesilate capsules thrice daily. Based on the treatment of the control group, patients in the observation added 1 dose Bushen Huoxue Mingnu decoction. All patients in both two groups received 12 weeks of treatment. Before and after treatment, vision and fundus examination were made and scores of TCM syndrome were graded. Levels of triglyceride (TG), total cholestero l(TC), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C), TNF-α and VEGF were detected. Result: The total effective rate of fundus lesions in the observation group was 86.3%, which was superior to that of 70.3% in the control group (P<0.05). After therapy, vision in the observation group was better than that in the control group (P<0.01). The inspection and TCM syndrome score in the observation group were lower than those in the control group (P<0.01). Levels of TG, TC and LDL-C declined after treatment in both two groups compared before (P<0.01). The LDL-C level in the observation group was lower (P<0.01), while the HDL-C standard was higher than those in the control group (P<0.01). After treatment, TNF-α and VEGF levels in the observation group were lower than those in the control group (P<0.01). Conclusion: Based on the conventional Western treatment, Bushen Huoxue Mingmu decoction could improve vision, signs and symptoms of NPDR patients. Its clinical curative effect is superior to using Western medicine treatment alone. The mechanism may be related to regulating lipid metabolism, reducing TNF-α and VEGF levels.关键词：diabetic retinopathy;proliferative;Bushen Huoxue Mingmu decoction;vascular endothelial growth factor;tumor necrosis factor α
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Tiaochong Jieyu decoction in treating depression of perimenopausal, and to investigate its influence on levels of estrogen (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and 5-hydroxytryptamine (5-HT). Method: One hundred and twenty patients were randomly divided into the control group (60 cases) and the observation group (60 cases) by a random number table. All patients in both groups received 2.5 mg tibolone tablets once daily. Patients in the control group added 2 flupentixol and melitracen tablets in the morning and noon. Patients in the observation added Tiaochong Jieyu decoction. All patients in two groups received 8 weeks of treatment. Before and at the fourth and the eighth week after treatment, sores of Hamilton depression scale (HAMD), Hamilton Anxiety Rating Scale (HAMA), Pittsburgh sleep quality index (PSQI) and Kupperman were graded. Levels of E2, FSH, LH and 5-HT were detected, and adverse reactions were monitored. Result: The total clinical efficiency of depression in the observation group was 90%, which was 86.67% in the control group. There was no statistical significance difference between the two groups. Total clinical efficient of syndrome of perimenopausal in the observation group was 91.67%, which was superior to that of 76.67% in the control group (P<0.05). There was no statistical significance difference for HAMD scores between the two groups. HAMA scores were lower at the eighth week after treatment, sores of PSQI and Kupperman were lower at the fourth and eighth week after treatment in the observation group than those in the control group (P<0.01). Besides, the results of HAMA, PSQI and Kupperman were more obvious in the observation group (P<0.01). After treatment, levels of FSH and LH were lower (P<0.01), and levels of E2and 5-HT were higher (P<0.01) in the observation group than those in the control group. The rate of adverse reaction was 18.3% in the control group, and there was no adverse reaction in the observation group. Conclusion: Based on the treatment of estrogen replacement therapy, the curative effect of Tiaochong Jieyu decoction was the same as flupentixol and melitracen tablets, while there was rare side effect. Moreover, there was obvious amelioration on sleep and syndrome in menopause, its mechanism of action may be related to regulating levels of neuroendocrine hormone.关键词：climacteric syndrome;depression;Tiaochong Jieyu decoction;5-hydroxytryptamine;oestrogen
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发布时间：2024-01-04 - 摘要：Objective: To observe the preventive effect of Yangyin Yifei decoction for assistance of radiotherapy in treating patients with acute radiation-induced pneumonia (RIP), and to investigate its influence on serous interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Method: One hundred and twenty-five patients were randomly divided into the control group (90 cases) and the observation group (93 cases). Patients in both groups received radiosurgery, and patients in the control group received symptomatic therapy without any traditional Chinese medicine treatment. Patients in the observation group received 1 dose Yangyin Yifei decoction. All patients in both two groups received 3 months of treatment. Incidence rate of RIP, injury degree and the occurrence time were recorded. Before and after the treatment, degree of dyspnea was evaluated by Watters method of point rating, and quality of life was evaluated by Karnofsky performance status score (KPS). Vital capacity (VC), forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) were detected. Levels of IL-1, IL-6 and TNF-α were also detected. Result: Incidence rate of RIP in the observation group was 41.11%, which was higher than that of 19.35% in the control group (P<0.05). Duration of RIP in the observation group was shorter than that in the control group (P<0.01). Radiation dose in the observation group was less than that in the control group (P<0.01). After treatment, patients' radioactive injury degree and Watters scores in the observation group were both lower than those in the control group (P<0.01). Levels of VC, FVC and FEV1 were higher than those in the control group (P<0.05). KPS score was higher than that in the control group (P<0.01). Levels of IL-1, IL-6 and TNF-α were lower than those in the control group (P<0.01). Conclusion: Assistance of Yangyin Yifei decoction could reduce the incidence rate of RIP and the injury degree caused by RIP, ameliorate dyspnea and stabilize patients' lung function, and its mechanism of action may be related to relieving inflammatory response.关键词：radiation induced pneumonia;Yangyin Yifei decoction;prevention
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发布时间：2024-01-04 - 摘要：Objective: Studies that have been published in China on the curative effect of Ginkgo biloba extract on acute cerebral infarction were searched and analyzed by Meta-analysis, in order to provide some evidences in treating cerebral infarction. Method: All the studies that have been searched and strictly evaluated through meta-analysis by the software Stata12.0, to evaluate the curative effect of Ginkgo biloba extract on acute cerebral infarction. Result: Totally 25 studies including 2 292 cases were analyzed. Compared with the control group, the OR merge value of neurological Ginkgo biloba extract defect efficacy of treatment of acute cerebral infarction was 3.35,95% CI (2.57,4.38), the OR merger value of neurological deficits therapy weighted mean difference (WMD) was -3.12,95%CI (-3.96,-2.28). Conclusion: Ginkgo biloba extract has the effect to improve the treatment of acute cerebral infarction and improve nerve function defect.关键词：cerebral infarction;ginkgo biloba extract;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To collate and analyze the Uyghur medicine syndrome classification of eczema and its medication discipline. Method: Using retrospective analysis of sorting, clinical medical records of eczema treated with Uyghur medicine within recent 5 years in 6 Uyghur medicine hospitals were collected. Uyghur medical symptom classification and treatment principles, methods, prescriptions and medications of eczema were collated and analyzed to discover eczema Uyghur medicine syndrome differentiation method and its medication discipline. Result: Among 621 cases of medical records meeting the inclusion criteria of eczema, there were 327 cases (52.7%) of abnormal balgham, followed by 239 cases (38.5%) of abnormal sapra, 33 cases (5.3%) of abnormal kan, and 22 cases (3.6%) of Abnormal Sawda. After munzij therapy or conditioning, mushil therapy, oral medication, topical medications and non-drug therapy of Uyghur medicine, 61 cases (9.8%) were cured with the treatment, 484 cases (77.9%) highly effected, 60 cases (9.7%) effected to some extent, 16 cases (2.6%) not effected at all. Conclusion: Uyghur medicine syndrome classification and medication discipline of eczema received in this project is in line with the actual clinical syndrome and that provides an objective basis for standardization of eczema's Uyghur medicine syndrome differentiation关键词：eczema;syndrome classification;medication discipline;clinical records collating
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to analyze the medication rule in herbal prescriptions for knee osteoarthritis by using Chinese medicine inheritance support system (TCMISS). Method: The herbal prescriptions for knee osteoarthritis from papers were collected,then the data were input into TCMISS and composition principles were analyzed by using data mining methods such as revised mutual information,complex system entropy cluster and unsupervised hierarchical cluster. Result: Based on the analysis of 263 herbal prescriptions,the frequency and meridian entry distributions of each herb were found, association rules among the herbs were computed,16 core combinations and 4 new prescriptions were mined from the database. Conclusion: The common Chinese medicines used for knee osteoarthritis included blood-activating and stasis-resolving medicine, blood-tonifying medicine, qi-tonifying medicine, wind-dampness-dispelling and cold-dispersing medicine. The major components of the Chinese medicines for knee osteoarthritis were the medicines which have the function of tonifying the kidney or activating blood. The meridian entry distributions of herbs are composed of liver meridian and kidney meridian. The method of treatment for knee osteoarthritis are to regulate the liver and supplement the kidney, activate blood and resolve stasis.TCMISS contribute to the study of composition principle lawof herbal prescriptions.关键词：knee osteoarthritis;composition principle of prescription;association rules;clustering algorithm
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the role of enriching Qi and nourishing Yin and promoting blood circulation adjunctive treatment in treating patients with diabetic cardiopathy (DC) by Meta-analysis. Method: The articles published from the January 1st 2000 to July 1st 2014 in databases of CNKI, VIP and Wanfang were collected. All studies adopted in Meta-analysis were screened and evaluated strictly. Results data from the articles were obtained. The works of Enriching Qi and Nourishing Yin and promoting blood circulation adjunctive treatment for reducing the levels of fasting plasma glucose (FPG) and improving the comprehensive clinical efficacy, Electrocardiogram (ECG) and blood support of cardiae muscle in DC patients were analyzed. Result: Eighteen studies were included in our Meta-analysis model. As shown in the results, enriching Qi, nourishing Yin and promoting blood circulation adjunctive treatment could reduce the serum levels of FPG [Z=2.90, P=0.004, MD=0.63,95%CI (0.20,1.05)], and the clinical comprehensive efficiency [Z=5.32, P<0.000 01, RR=1.32,95%CI(1.19,1.46), ECG [Z=4.84, P<0.000 01, RR=1.31,95%CI(1.17,1.45)] and the blood support of cardiae muscle [(Z=8.34, P<0.000 01, RR=1.83,95%CI(1.59,2.11)] in DC patients were improved significantly. Conclusion: Enriching Qi, nourishing Yin and promoting blood circulation adjunctive treatment is an effective medical method for DC.关键词：diabetic cardiopathy;Meta-analysis;enriching Qi nourishing Yin and Promoting blood circulation treatment;efficacy
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发布时间：2024-01-04 - 摘要：Spherical crystallization is a technology to obtain spherical drugs with large particle size by controlling polymerization process of drug molecules during crystallization.As a new method of particle design,this technique can improve powder properties,including flowability,compressibility,bioavailability and so on,control process parameters and obtain spherical particles with desired particle size distribution.Process parameters during crystallization includes temperature,stirring rate,type and amount of solvents etc.This paper focuses on four classical techniques for obtaining spherical particles and give viewpoint on its industrial enlargement.关键词：spherical crystallization;powder modification;spherical agglomeration;quasi-emulsion solvent diffusion;ammonia diffusion method;crystalline copolymer;crystal habit
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发布时间：2024-01-04 - 摘要：The Food and Drug Administration (FDA, USA) has issued a guidance that only the therapeutically active enantiomer of a chiral drug be brought to market. Recently, chiral molecules occupy a large proportion of therapeutic agents, chiral drugs have similar structure so powerful means of chiral drug detection and separation are needed. Supercritical fluid chromatography (SFC) has several advantages over HPLC:reduced viscosity, which decreases the pressure drop across the column, allowing faster separation;increased diffusivity, which results in sharper peaks and, thus, increased resolution;lower solvent consumption and easy solute recovery, which makes SFC particularly useful in preparative modes. SFC is increasingly used for analytical, semi-preparative and preparative purification of chiral compounds, which makes it has attracted wide interests in the pharmaceutical analysis as a useful separation technique for enantioselective separations. This review focuses mainly on the examples of enantiomer separations by SFC with mobile phases and chiral stationary phases, so as to facilitate the selection of mobile phase and stationary phase and the optimization of chromatographic conditions.关键词：supercritical fluid;supercritical fluid chromatography;chiral stationary phase;enantioselective separations
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发布时间：2024-01-04 - 摘要：Gold immunochromatographic assay (GICA) is established by combining the colloidal gold immunoassay with chromatographic assay in solid membrane and has been widely applied in research field of food, agriculture, biology and clinical medicine. GICA has been applied in detection of natural small molecules based on preparation of specific monoclonal antibodies and has the advantages of simplicity, rapidity, less sample consumption and visual identification results with naked eyes. The paper introduced the principle of GICA on account of competitive combination of antigen and antibody, and summarized the research report on application of GICA in detection of different types of natural small molecules based on the domestic and abroad literatures in recent years, indicating that GICA has great significance on improvement of quality control system of natural medicines. Moreover, the advantages and disadvantages, technical obstacles and improvement of GICA are discussed in order to provide a salutary reference for quality control of natural medicines.关键词：gold immunochromatographic assay(GICA);natural small molecules;monoclonal antibody;detection and analysis
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发布时间：2024-01-04 - 摘要：The nature and taste theory is both the important component of traditional Chinese medicine(TCM) system and the core element of TCM herbal system. It plays a guiding role in the selection of the optimized herbs in the TCM clinical application. To further uncover the modern connotation of the nature and taste theory, grants in this regard have been set by Ministry of Science and Technology, State Administration of Traditional Chinese Medicine and National Natural Science Foundation of China. The current situation of four natures and five tastes of the Chinese herbs was systemically reviewed from the aspects of its biological effects and ingredients based on the articles related to the study of herbal natures published in the recent 20 years. In this paper, the authors put forward the disadvantages and problems around the herb natures study combined with the recent findings and experience accumulated in the process of the herb natures study conducted in the authors' lab. This review will pave the way for the further study of four natures and five tastes of the Chinese herbs.关键词：Chinese herbs;nature and taste theory;study progress
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发布时间：2024-01-04 - 摘要：Near infrared spectroscopy in the application of traditional Chinese medicine(TCM) research and production develops rapidly although started a little bit late. In recent years, the quality problem of TCM has been controversial, and TCM market is in quite a chaos. It is an effective way to take an analysis technique to identify the false and inferior Chinese medicinal materials to control the quality to improve the present situation of TCM market, and to ensure the safety of people's drug usage. As a new analysis technique, near infrared spectroscopy in the application of identification and content determination of TCM becomes more and extensive. It is a highly effective, convenient and nondestructive and environment-friendly method to perform qualitative analysis and determine the content of TCM by using NIRS. It is of great significance and value to use NIR in the qualitative identification and quantitative detection of Chinese medicine. However, the effective information extraction rate by near infrared spectrum is low, and it is relatively difficult to identify the weak signal with relatively low sensitivity, which is not suitable for trace analysis. It needs to improve in the future. In this article, the research progresses in recent years on the application of near infrared spectral analysis technology in the identification of authenticity, adulteration, production area, and variety of TCMs, and content determination were reviewed.关键词：near infrared;traditional Chinese medicine identification;content determination
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