最新刊期
卷 21 ， 期 13 ， 2015
- 摘要：Objective: To investigate effect of compatibility of Paeoniae Radix Alba and Aconiti Radix Cocta on transdermal permeation role of paeoniflorin. Method: Modified Franz diffusion cell was adopted with excised mice skin as transdermal barrier,through in vitro percutaneous absorption test,the content of paeoniflorin was determined by HPLC,then cumulative amount,permeation rate and lag time were calculated in order to obtain penetration curve equation. Result: Steady-state permeation rates of paeoniflorin according to groups of Paeoniae Radix Alba,Paeoniae Radix Alba-Aconiti Radix Cocta,Paeoniae Radix Alba+1% azone,Paeoniae Radix Alba+1% oleic acid and Paeoniae Radix Alba+1% propylene glycol were 0.818,2.532,3.974,0.621,0.430 μg·cm-2·h-1;apparent permeability coefficients of them were 2.538×10-8,7.856×10-8,12.328×10-8,1.928×10-8,1.335×10-8 cm·s-1;lag times were 6.168,0.071,10.462,1.487,11.827 h,respectively. Conclusion: Compatibility of Paeoniae Radix Alba and Aconiti Radix Cocta is benefit to transdermal permeation of paeoniflorin.关键词：compatibility;transdermal absorption;Paeoniae Radix Alba;Aconiti Radix Cocta;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To investigate transport characteristics of main ingredients from Sophorae Tonkinensis Radix et Rhizoma in Caco-2 cell model and provide a reference for further studies on its in vivo absorption characteristics. Method: Transport of main ingredients in Sophorae Tonkinensis Radix et Rhizoma from apical side to basolateral side was studied by Caco-2 cell monolayer model.Permeability of oxymatrine and matrine from these two sides was determined by UPLC-MS,mobile phase was 0.1% formic acid-acitonitrile by gradient elution at flow rate of 0.4 mL·min-1.Mass spectrum was operated in positive mode with oxymatrine (m/z±H) 265.1 and matrine(m/z±H) 249.1,their apparent permeability coefficients (Papp) were calculated. Result: At 120 min after dosing,Papp of matine was (11.43±0.41)×10-6 cm·s-1,indicating that transmittance of matine was high;while Papp of oxymatine was (3.91±0.70)×10-6 cm·s-1,indicating that transmittance of oxymatine was lower.Twenty constituents were detected in aqueous extract of Sophorae Tonkinensis Radix et Rhizoma,five constituents of them(P2,P4,P6,P7,P25) including matrine had high levels of transmittance,six constituents(P3,P5,P9,P11,P14,P27) had medium levels of transmittance,these other substances had low levels of transmittance or had no transmittance at all,which included oxymatrine. Conclusion: Fifty-five percent of main components are easily transported through momolayer.Among all components,matrine can be absorbed easily in vitro cell model,however,permeability of oxymatrine is poorer than matrine in vitro.This study suggests that matrine may be one of major components that was aborsopted in vivo of Sophorae Tonkinensis Radix et Rhizoma.关键词：Sophorae Tonkinensis Radix et Rhizoma;Caco-2 cell monolayer model;matrine;oxymatrine;apparent permeability coefficient
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发布时间：2024-01-04 - 摘要：Objective: To provide theoretical and experimental basis for industrial production of Shenbo lotion by optimizing its preparation technology. Method: Volatile oil from pine needle and Schizonepetae Herba was extracted by steam distillation method,extraction technology of volatile oil was optimized through single-factor test.With comprehensive score of total alkaloids concentration and solid content as index,orthogonal design was optimized water extraction process by taking water consumption,extracting times and time as factors,concentrating and molding processes were screened by single factor test. Result: Optimum preparation process was as following:extracted 5 h with 14 times the amount of water,added 0.1% polysorbate 80 to solubilizing oil.The rest medical materials with herb residue were boiled thrice with 8 times the amount of water,1 hour for each time.Filtrate was concentrated by atmospheric pressure or reduced pressure,then adding 0.3% sodium benzoate as preservative and purifying after refrigerating 48 hours.The concentration of total alkaloids was no less than 195 mg·L-1,and its retention rate was no less than 85%. Conclusion: This preparation technology is stable and feasible,it serves as a theoretical basis for standardized production of Shenbo lotion.关键词：Shenbo lotion;matrine;oxymatrine;molding process;preservative;emulsifier
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of Maodongqing dispersible tablets. Method: Taking alcohol extract of Ilex pubescens as raw material,fillers,disintegrating and wetting agent were screened by single factor test.With disintegration time linited as index,central composite design-response surface methodology was adopted to optimize formulation process by taking amouts of microcrystalline cellulose (MCC),cross-linked povidone (PVPP) and sodium carboxymethyl starch (CMS-Na) as factors. Result: The best preparation process was as following:alcohol extract of 40%,calcium hydrogen phosphate of 27%,MCC of 18%,PVPP of 9%,CMS-Na of 5%,PVP of 3%,sucralose of 0.2%,magnesium stearate of 0.8%.disintegration time limited was 84.7 s,whose deviation was 3.3% by comparing with the predictive value. Conclusion: Preparation process of Maodongqing dispersible tablets is simple with short disintegration time limited and good preparation stability,which is suitable for industrial production.关键词：Ilex pubescens;dispersible tablets;disintegration time limited;filler;wetting agent
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发布时间：2024-01-04 - 摘要：Objective: To ascertain extract with relative molecular weight cutoff ≤1 kDa as the final product,then optimize extracting combination of herbs in Banxia Baizhu Tianma Tang with semi-bionic enzyme method. Method: Herbs combination was divided into 15 groups,taking yields of gastrodine,glycyrrhetinic acid,total flavonoids,extract and total area of extract HPLC as indexes,extracting combination of herbs in Banxia Baizhu Tianma Tang with semi-bionic enzyme method was optimized by comprehensive scoring. Result: The best combination of six herbs was as following:mixing extraction of Pinelliae Rhizoma,Gastrodia Rhizoma and Atractylodis Macrocephalae Rhizoma,mixing extraction of Poria,Citri Exocarpium Rubrum and Glycyrrhizae Radix et Rhizoma.Yields of gastrodine,glycyrrhetinic acid,total flavonoids,extract and total area of extract HPLC were 1.552 8 mg·g-1,189.864 2 μg·g-1,6.767 4 mg·g-1,0.238 1 g·g-1,7 566.3. Conclusion: This optimized herbs combination is rational and feasible for providing a reference for extraction process of Banxia Baizhu Tianma Tang.关键词：Banxia Baizhu Tianma Tang;semi-bionic enzyme method;gastrodine;glycyrrhetinic acid;total flavonoids
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Bushen Quyu granules. Method: UV was adopted to determine contents of total polysaccharides and total flavonoids with detection wavelength at 489 nm and 510 nm.HPLC was used to determine the content of stilbene glycoside with mobile phase of acetonitrile-water(22: 78) and detection wavelength at 320 nm.Taking total polysaccharides yield as index,orthogonal test was adopted to investigate effects of solid-liquid ratio,extraction time and times,alcohol precipitation concentration on water extraction process;taking comprehensive score of yields of total flavonoids,stilbene glycoside and extract as index,effects of ethanol concentration,ethanol amount,extraction time and times on alcohol extraction process were optimized by orthogonal test. Result: Optimum water extraction process was as follows:soaked 1 h and refluxing extracted thrice with 12 times the amount of water for 1.5 h each time,alcohol precipitation concentration of 80%;yield of total polysaccharides reached 8.682%.Optimum ethanol extraction process was:refluxing extracted thrice with 10 times the amount of 80% ethanol for 1.5 h each time;yields of total flavonoids,stilbene glycoside and extract reached 660.19 mg·g-1,145.91 μg·g-1,11.81%,respectively. Conclusion: These optimized extraction processes are repeatable and feasible to provide basis for industry production of Bushen Quyu granules.关键词：Bushen Quyu granules;total polysaccharides;total flavonoids;stilbene glycoside
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发布时间：2024-01-04 - 摘要：Objective: A comparative study on inclusion effect of purity gum and β-cyclodextrin(β-CD). Method: With inclusion rate of volatile oil and yield of inclusion complex as indexes,purity gum and β-CD as inclusion materials,four kinds of inclusion technique were adopted to inclusion of volatile oil from Notopterygii Rhizoma et Radix,Forsythiae Fructus and Magnoliae Flos,inclusion process was optimized by single factor test,differential scanning calorimetry (DSC) and infrared spectrum was used to characterize technology of inclusion complex. Result: Inclusion rate of purity gum was significantly higher than β-CD,but recovery of inclusion complex of purity gum was slightly lower than β-CD.DSC diagram of purity gum inclusion curve did not appear characteristic peaks of volatile oil showed that oil has been involved.Maximum absorption peak of purity gum inclusion in infrared spectrum graph with respecting to purity gum have migrated to high frequency,it showed purity gum inclusion was a new biological phase,and inclusion effect was stable. Conclusion: Taking purity gum as inclusion material,magnetic stirring method for inclusion technique to prepare inclusion complex,not only process is simple,and has good effect of inclusion.关键词：starch sodium octenylsuccinate;ultrasonic method;ultrasonic cell-break method;high pressure homogenization;magnetic stirring method;β-cyclodextrin
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology of total flavonoids from Hyssopus officinalis by macroporous resin. Method: Adsorption and desorption capacity of total flavonoids was taken as index,11 different types of macroporous resins were screened,static adsorption-elution test were carried out.Single factor test was used to investigate purification technology of total flavonoids from H. officinalis. Result: AB-8 macroporous resin was the most suitable one for purification of total flavonoids in H. officinalis.Optimum purification parameters were as followings:the sample concentration of 2.56 g·L-1,sample flow rate of 4 BV·h-1,diameter-high ratio of resin bed 1: 5,washed impurity with 5 BV of water,elution flow rate of 5 BV·h-1 and discarded eluent,eluted with 9 BV of 70% ethanol at 4 BV·h-1.Purity of total flavonoids reached 65.6%. Conclusion:This purification technology is stable,feasible and suitable for purification of total flavonoids from H. officinalis.关键词：Hyssopus officinalis;total flavonoids;macroporous resins;rutin
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发布时间：2024-01-04 - 摘要：Objective: To determine the active extracts and uncover the material basis of Sanhuang Xiexin Tang, comparing the anti-inflammatory effect of its water and ethanol extracts. Method: Use the xylene-induced auricular swelling of mouse as screening model: oral administration, and calculate the inhibition degree of swelling to determine the active extracts. Meanwhile, conduct the component analysis for active extracts. Result: Compared with model group, decrease of swelling in different groups is observed, the swelling of mouse is significantly lower of ethanol extracts than water extracts (P<0.01) and the inhibition degree is about 46%. The swelling of mouse in the medium dose and high dose of ethanol extracts are significantly lower than water extracts (P<0.05), the inflammatory effect of ethanol extract is better than water extracts. The ratio of flavone, anthraquinone and alkaloid in ethanol extracts is 9: 2: 5. Conclusion: The inflammatory effect of ethanol extracts is obvious, which has something to do with the component ratio within the extracts. And ethanol extracts is determined as the active extracts of Sanhuang Xiexin Tang.关键词：Sanhuang Xiexin tang;extracts;anti-inflammatory;component analysis
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发布时间：2024-01-04 - 摘要：Objective: To identificate different origins and categories of commodity edible bird's nest (EBN) by DNA barcoding technique fastly and acurrately. Method: Total genomic DNA was isolated from different origins and categories of commodity EBN. Nicotinamide adenine dinucleotide dehydrogenase subunit 2 (ND2) gene sequences were amplified and sequenced.Identification were performed using similar Blast from GenBank,nearest distance and neighbor-joining (NJ) methods. Result: NJ phylogenetic tree showed 31 white or silver EBN samples which were clustered with Aerodramus fuciphagus;a black EBN sample was clustered with A. maximus.However,among white or silver EBN samples,there were 8 samples clustered into a separate branch with support rate of 82%,which were identified by cytochrome b (Cytb) gene as the subspecies (A. fuciphagus germani),and sequence alignment showed 2 parsimony-informative sites.This result showed that segment of ND2 sequence can identify subspecies of A. fuciphagus. Conclusion: ND2 sequence is efficient barcode for identifying origin species of EBN.关键词：edible bird's nest;DNA barcode;nicotinamide adenine dinucleotide dehydrogenase subunit 2;genetic identification
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发布时间：2024-01-04 - 摘要：Objective: To investigate quality differences and accumulation law of steroidal saponins within Paris polyphylla var. yunnanensis, to provide a basis for quality evaluation and quality germplasm screening of the medicinal material. Method: UPLC was employed to determine contents of polyphyllin Ⅰ,Ⅱ,Ⅵ and Ⅶ from roots,stems and leaves in the same population of P. polyphylla var. yunnanensis.UPLC fingerprint of different parts was established,quality differences of P. polyphylla var. yunnanensis and its accumulation law of steroidal saponins were analyzed. Result: Contents of total saponins from roots and leaves in the same population of P. polyphylla var. yunnanensis were more than 0.6%.There were significant differences about contents of polyphyllin Ⅰ,Ⅱ and Ⅶ,but polyphyllin Ⅵ showed no significant difference.Five common peaks of UPLC fingerprint were selected in roots,stems and leaves,similarity of characteristic peaks were above 0.753. Conclusion: Polyphyllin Ⅰ,Ⅱ,Ⅵ and Ⅶ are synthesized,stored and distributed in the chloroplasts of green plants,then transported down by stems and majorly accumulated in leaves and rhizomes at last,accumulation law of total saponins content in different parts is leaves>roots>stems.There are large differences about contents and species of main active ingredients among roots,stems and leaves of P. polyphylla var. yunnanensis.关键词：Paris polyphylla var. yunnanensis;total saponins;quality evaluation;accumulation of active ingredients;polyphyllin
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发布时间：2024-01-04 - 摘要：Objective: Study the chemical constituents of Rumex hastatus roots. Method: Isolation and purification from the n-butyl alcohol extract of R. hastatus roots were carried out on column chromatography silica gel and Sephadex LH-20. Result: Their structures were elucidated on basis of physicochemical properties and spectral data. Ten compounds were isolated and identified as nepodin-8-O-β-d-glucopyranoside (1), 6-hydroxymusizin-8-O-β-d-glucopyranoside (2), torachysone-8-O-β-d-glucopyranoside (3), isorhamnetin (4), emodin-8-O-β-d-glucopyranoside (5), 7-hydroxy-5-methyl-coumarin-4-O-β-d-glucopyranoside (6), nepodin-8-O-β-d-(6'-O-acetyl)-glucopyranoside (7), 2-(2',6'-dihydroxybenzoyl)-5-methylbenzoic acid-3-O-β-d-glucopyranoside (8), polydatin (9) and rumexoside (10). Conclusion: Compounds 1, 2, 3, 4, 5, 9 and 10 were isolated from this plant for the first time.关键词：Rumex hastatus;n-butyl alcohol;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To establish an UPLC method for fingerprints of Artemisae Argyi Folium, and provide reference for quality control of it. Method: The RP-UPLC method was achieved on a uitimate-C18(2.1 mm×100 mm, 1.8 μm) column with mobile phase consisting of acetonitrile-0.10% acetic acid water with gradient elution. Flow rate was set at 0.20 mL·min-1. The detection wavelength was set at 290 nm. Then established the fingerprints based on samples from different sources, and the similarity was analyzed. Result: The UPLC fingerprint was established with 36 common peaks. However similarity of the UPLC fingerprints of Artemisae Argyi Folium and non-medicinal parts and adulterants were lower than 0.700 and the common peaks less than 36. Conclusion: A simple, reproducible, and characteristic UPLC fingerprint was developed, which can be used to identify of Artemisae Argyi Folium. The difference of fingerprint indicated that of chemical compositions of Artemisae Argyi Folium, non medicinal parts and adulterants were varied, it should not be mixed.关键词：Artemisae Argyi Folium;UPLC;fingerprint;confusion
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发布时间：2024-01-04 - 摘要：Objective: To establish a quantitation of triacylglycerols in Cannabis Semen by non-aqueous reversed-phase high-performance liquid chromatography. Method: The chromatography was carried out on Hypersil ODS2 column (4.6 mm×250 mm, 5 μm) with acetonitrile-isopropanol(75: 25) as the mobile phase at a flow rate of 1.0 mL·min-1, the detection wavelength was set at 205 nm. Result: The linear rang of linolein was 0.016-0.33 g·L-1 (r=0.999 8). The average recovery was 97.6%, RSD 1.4%. Conclusion: A non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) was established for determination triacylglycerols in Cannabis Semen.关键词：Cannabis Semen;NARP-HPLC;Ultraviolet detector;triacylglycerol;quantitation
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发布时间：2024-01-04 - 摘要：Objective: In order to apply the guiding basis for harvest, management, storage and process of Paris polyphylla var. yunnanensis rhizome, chemical composition changes of P. polyphylla var. yunnanensis rhizome during the storage period was studied. Method: Nine P. polyphylla var. yunnanensis rhizome samples were sealed up and stored at room temperature for one year. The chemical compositions of P. polyphylla var. yunnanensis rhizome samples were analyzed with HPLC method before and after storage. Result: The chemical compositions of P. polyphylla var. yunnanensis rhizome were changed significantly after stored for one year, which could cause the changes of the medicinal quality and efficacy. Among them, the content of saponin Ⅵ in P. polyphylla var. yunnanensis rhizome increased;the content of saponinⅡ decreased;and no regularity was observed for the contents changes of saponinⅠ and saponin Ⅶ. Conclusion: According to the chemical composition changes of P. polyphylla var. yunnanensis rhizome, long storage period should be avoided to keep its medicinal quality and efficacy.关键词：Paris polyphylla var. yunnanensis rhizome;storage years;saponins;HPLC;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To obtain the active compounds of Rabdosiae Rubescontis Herba on plasma recalcification time. Method: EtOAC and methanol extracts were separated and purified by several kinds of chromatography, obserivedthe plasma recalcification time in vitro to screen theactive compounds of Rabdosiae Rubescontis Herba. Result: Six compounds were isolated and identified from the two extracts above. Compared with vitamin K1 group, oridonin (1) and rosmarinic acid (2) had no significant difference on plasma recalcification time(P>0.05). Compared with control group(200.87±2.45) s, caffeic acid (3) had no significant difference (P>0.05). The plasma recalcification times of salicylic acid (4)(214.77±5.8) s andferulic acid (5)(233.43±6.88) s were higherthan that of the control group (252.23±2.6) s,and lower than that of vitamin K1 group(158.2±2.33) s.The plasma recalcification time of vanillic acid (6)(286.2±4.53) s was longer than that of thecontrol group (252.23±2.6) s,and lower than that of breviscapine group (309.87±2.34) s. Conclusion: Oridonin, rosmarinic acid, salicylic acid andferulic acid had procoagulant activity, vanillic acid had anticoagulant effect.关键词：Rabdosiae Rubescontis Herba;plasma recalcification time;active constituents controlcontrol
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method for determining five constituents (ephedrine hydro-chlorid,pseudoephedrine hydrochloride,amygdalim,baicalin and praeruptorin A) in Xiaoer Qingfei Huatan oral solution. Method: Five constituents were analyzed by ZORBAX SB-C18(4.6 mm×250 mm,5 μm) with acetoni-trile-water (containing 0.1% phosphonic acid) as mobile phases in gradient elution, 0.8 mL·min-1 as flow-rate and 35 ℃ as temperature of column. The detection wavelengths were at 210 nm for ephedrine hydrochloride and pseudoephedrine hydrochloride,225 nm for amygdalim, 277 nm for baicalin and 321 nm for praeruptorin A respectively. Result: Five constituents were seperated perfectly and good linear relationships were obtained. The recoveries were between 98.52%-99.93%%. Conclusion: The developed method is simple,specific,accurate and with good reproducibility,which can be used for quality control of Xiaoer Qingfei Huatan oral solution.关键词：Xiaoer Qingfei Huatan oral solution;ephedrine hydrochloride;pseudoephedrine hydrochloride;amygdalim;baicalin;praeruptorin A
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发布时间：2024-01-04 - 摘要：Objective: To establish the chromatographic fingerprint for Chaihu Longgu Muli Tang and n-butanol extraction by RP-HPLC-DAD. Method: HPLC method was applied to establish the chromatographic fingerprint. The separation was performed on a Thermo Fisher Hypersil GOLD C18 column with a gradient elution composed of acetonitrile and 0.1% phosphoric acid. The column temperature was 35 ℃ and the flow rate was 1.0 mL·min-1. The detective wavelength was 276,210 nm. Result: The common pattern of chromatographic fingerprint of Chaihu Longgu Muli Tang and n-butanol extraction was established,and 4 peaks of them were identified respectively. In decoction,the 8th,12th,14th and 15th peaks were assigned to baicalin,cinnamic acid,cinnamaldehyde and rhein respectively by comparison with the retention times and UV spectra of reference substances. The 16th,17th,28th and 29th peaks were assigned to ginsenoside Rg1,ginsenoside Re,saikosaponin a and saikosaponin b2 respectively of n-butanol extraction. The similarity of 10 batches of Chaihu Longgu Muli Tang and n-butanol extraction were all more than 0.88. Conclusion: The established HPLC fingerprint of Chaihu Longgu Muli Tang has desirable precision,reproducibility,and can provide a basis for quality evaluation of Chaihu Longgu Muli Tang.关键词：Chaihu Longgu Muli Tang;n-butanol extraction;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To identify chemical composition in Ganlibao capsules by high-performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS) method. Method: The separation was performed on SHISEIDO MG C18 reverse phase column(3.0 mm×100 mm,3 μm). The mobile phase consisted of water containing 0.1% formic acid (A)-acetonitrile (B) was used for gradient eluting (0-10 min,5%-20%B;10-30 min,20%-70%B). Temperature of column was 25 ℃, the flow rate was 0.6 mL·min-1 and post-column split ratio was 2: 1.Time-of-flight mass spectrometer (TOF/MS) and electrospray ion source (ESI) were applied for qualitative analysis under positive and negative ion mode, and mass scan range was m/z 100-1 000. Result: The 30 components of Ganlibao capsules were identified by HPLC-TOF/MS. Conclusion: This method is simple and rapid for elucidating the constituents of Ganlibao capsules, which provides the basis for the research of active composition and quality control for this prescription.关键词：Ganlibao capsule;chemical composition;identification;TOF/MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for the simultaneous determination of three main flavonoids in Sabina chinensis and Junipeius formosana. Method: Samples were extracted by 70 % methanol with ultrasonic process. The HPLC was performed on an CAPCELL PAK C18 column (4.6 mm×250 mm, 5 μm)with 0.2%H3PO4acetonitrile-0.2%H3PO4 water as mobile phase. The flow rate was 1.0 mL·min-1 and the column temperature was kept at 40 ℃. The detection wavelength was at 360 nm. Result: The linear range of rutin was 0.004 9-0.146 4 μg(r=0.999 9);the linear rang of quercetin-3-rhamnoside was 0.009 4-0.281 4 μg(r=0.999 8)and the linear range of kaempferol was 0.005 4-0.162 0 μg(r=0.999 8). The average recoveries of rutin, quercitrin;and kaempferol were 100.12%(RSD 2.6%),96.35%(RSD 0.7%),96.13%(RSD 0.5%),respectively. Conclusion: Three flavonoids reached baseline separation within 40 min. This method has advantages of less analytic time, good stability and accuracy of operation, and has certain reference value for quality control of Junipeius formosana and Sabina chinensis herb.关键词：Sabina chinensis;Juniperus formosana;HPLC;flavonoid;rutin;quercitrin;kaempferol
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发布时间：2024-01-04 - 摘要：Objective: To establish a UPLC-MS-MS analysical method for simultaneous determination of concentrations of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside,l-deoxynojirimycin,cassiaside,rubrofusarin gentiobioside,aurantio-obtusin,chryso-obtusin,resveratrol in rat plasma and to study their pharmacokinetic characteristics after taking Zishen Qinggan Daiping formula in rats. Method: Acidified plasma samples were precipitated for protein with methanol.Waters Acquity UPLC BEH C18 column was employed with mobile phase of 0.1% formic acid-water and acetonitrile for gradient elution.Detection was carried out by multiple reaction monitoring (MRM) positive ion mode with ESI ionization source. Result: Seven constituents showed a good linear relationship with extraction recovery ranging between 94.83%-106.58% and a high intra-and inter-day precisions and accuracy.Their pharmacokinetic parameters in model group rats were AUC0-∞ of (326.65±26.66) μg·L-1·h-1,t1/2 of (3.64±1.69) h,Tmax of 0.33 h,CLz/F of (60.56±5.32) L·h-1·kg-1,Vz/F of (325.13±167.18) L·kg-1,Cmax of (169.25±18.02) μg·L-1. Conclusion: This mentioned method is specific,rapid,sensitive and suitable for pharmacokinetic studies of Zishen Qinggan Daiping formula in rats.关键词：Zishen Qinggan Daiping formula;pharmacokinetics;stilbene glycoside;cassiaside;resveratrol
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发布时间：2024-01-04 - 摘要：Objective: To optimize the most effective component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma on hepatoma carcinoma, and to reveal its antitumor mechanisms. Method: Human hepatoma cell SMMC-7721 was used as object and human normal liver cell line L-O2 was used as control in this study. The inhibiting effect on SMMC-7721 and the protective effect on L-O2 were performed by CCK-8 assay, and the most effective component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma was optimized by using the orthogonal design method. The effect on SMMC-7721 and L-O2 viabilities were assayed by using Real-time cell assay and the time-effect relationship was analyzed. Cell apoptosis was measured by high content screening using Annexin V/PI double staining. Result: The results demonstrated that the contents of total salvianolic acid, total saponins Ginseng Radix et Rhizoma and ginseng polysaccharide doses were 10, 10, 5 mg·L-1, respectively, in the optimizing component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma . Furthermore, the component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma could significantly decrease the SMMC-7721 cell viability and induce SMMC-7721 cell apoptosis, while there was no significant difference on the normal liver cell. Conclusion: The component formula of Salviae Miltiorrhizae Radix et Rhizoma and Ginseng Radix et Rhizoma has potentially specific and selective anti-hepatoma carcinoma effect. Its mechanism may be related to the inhibition of hepatoma cell proliferation and induction of cell apoptosis.关键词：Salviae Miltiorrhizae Radix et Rhizoma;Ginseng Radix et Rhizoma;component formula;hepatoma carcinoma
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antidepressant activity of qingyangshenylycosides (QYS) in the chronic unpredictable mild stress rats (CUMS), and to explore its mechanism by the comparison of concentration of monoamine neurotransmitters in brain tissue. Method: A total of 54 male adult Wistar rats were randomly assigned into 6 groups:the normal group, the model group, the fluoxetine group, the low-, medium-and high-dose QYS groups (0.625, 2.5, 10 mg·kg-1) of 9 rats each. The CUMS model was induced in rats by receiving unpredictable mild stress for 21 days. Meanwhile, the corresponding medicines were intragastrically administered to rats. The antidepressant effects of QYS on CUMS rats were evaluated by using the tail suspension test, forced swimming test and open field test. The concentrations of monoamines in rat brain including noradrenaline (NE), 5-hydroxytryptamine (5-HT) and dopamine (DA) were determined by using high-performance liquid chromatograph with electrochemical detection method. Result: Compared with the normal group, the immobility time in tail suspension and forced swimming test increased, the autonomic activities decreased, the concentrations of NE, 5-HT and DA in frontal cortex decreased in the model group. Compared with model group, the immobility time in the tail suspension and forced swimming test decreased, the autonomic activities increased, the concentrations of NE, 5-HT increased in the high-dose QYS group (P<0.05, P<0.01). Conclusion: QYS at 10 mg·kg-1 produced reliable antidepressant effect in CUMS rats. The mechanism may be related to improving the concentrations of NE and 5-HT in the CUMS rat brain.关键词：antidepressant effect;chronic unpredictable mild stress;qingyangshenylycosides;locomotor activity;monoamine neurotransmitters
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of anti-inflammation of Jiawei Huoxiang Zhengqi soft capsule (JWHX) on dextran sulphate sodium salt (DSS) induced colitis model in mice. Method: Sixty ICR male mice were randomly divided into normal group, model group, JWHX in high, middle and low dose (0.60, 0.30, 0.15 g·kg-1) groups. DSS 35 g·L-1 was used to induce colitis model in mice, at the same time mice was given drug by intragastric administration for 7 days. The disease activity index (DAI) was scored daily. Mice was killed on the day 7, the histopathological score of each mouse colon was evaluated, the content of myeloperoxidase (MPO) in colonic tissue was measured. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) were measured. Result: Compared with normal group, in the model group the DAI score and the pathological score increased significantly, and the content of MPO, IL-6, TNF-α increased significantly in the colon, and colon length decreased significantly in mice(P<0.01). Compared with the model group,high and middle dose JWHX could significantly decrease the DAI score and the pathological score, and significantly increased length of colon and reduce the content of MPO in the colon, IL-6, and TNF-α levels(P<0.05,P<0.01);Low-dose JWHX could significantly reduce the DAI score, increased length of colon, and significantly reduce the content of MPO in the colon(P<0.05). Conclusion: JWHX has certain therapeutic effect on DSS-induced colitis in mice, and its mechanism may be related to the inhibition of the IL-6,TNF-α of inflammatory factor.关键词：Jiawei Huoxiang Zhengqi soft capsule;dextran sulphate sodium salt;colitis;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the reversal or effect of multi-drug resistance by nitidine chloride(NC) on A2780 Taxol cell. Method: The reversal efficacy of NC was determined by MTT assay. The expressionof multidrug resistance 1 (MDR1) mRNA was assessed by reverse transcription-polymerase chain reaction (RT-PCR).The structural changes of A2780 Taxol cell were examined with hematoxylin and eosin staining(HE).The apoptosis of A2780 Taxol cell was detected by TUNEL assay. Result: The resistance fold of A2780 Taxol cell was 1.87,1.07,3.25 for adriamycin(ADM),etopaside(VP-16),hydroxycamptothecin(HCPT).The 50% inhibition concentration(IC50) value of A2780 Taxol cell was reduced by NC(0.375,0.75,1.5 mg·L-1) combined with ADM,VP-16 or HCPT, and NC could decrease the expression of MDR1-mRNA, change the structural of A2780 Taxol cell. The apoptosis index (AI) was(58.03±1.46)% detected by TUNEL assay. Conclusion: Nitidine chloride can reverse the multidrug resistance of A2780 Taxol cell.关键词：nitidine chloride;multidrug resistance;A2780 Taxol cell;reversol
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发布时间：2024-01-04 - 摘要：Objective: To observe the role of Atractylodis Rhizoma extract on protecting gastric mucosa and gastrointestinal dysfunction of rats with spleen deficiency syndrome,and to elucidate the mechanism of Atractylodis Rhizoma extract regulating spleen deficiency syndrome. Method: Male rats were fed with Xiaochengqi decoction and hunger to establish spleen deficiency syndrome models.Then the animal were randomly divided into model group, Atractylodes Rhizome extract high, middle and low dose group(20,10,5 g·kg-1), Domperidone group 5 mg·kg-1. After 10 days of treatment, charcoal gavage method was used o determine residual rate of stomach and small intestinal propulsion ratio of the rats, hematoxylin and eosin(HE) staining was used to observe the gastric mucosa pathological and histological changes,like gastric mucosa tissue cells of glandular stomach area and ultrastructural alterations of intercellular junctions with transmission electron microscopy method, laser Doppler blood flow meter was used to measure gastric mucosal blood flow, immunohistochemical was used to determinate the expression of trefoil factor 1(TFF1) in gastric mucosa and c-kit expression in colon tissue volume. Result: Compared with the normal group, gastric mucosal morphology had significantly abnormal change in model group;gastric mucosal blood flow, gastric mucosa TFF1 and colon tissue volume c-kit expression were decreased (P < 0.01);residual rate of stomach was increased and small intestinal propulsion ratio was decreased(P < 0.05, P < 0.01). After intervention of Atractylodis Rhizoma extract,those indicators were improved(P<0.05,P<0.01). Conclusion: Atractylodis Rhizoma extract can inhibit the gastric mucosal damage of spleen deficiency rat, protect and repair the damaged mucous membrane organization,and improve the gastrointestinal function of spleen deficiency rats.关键词：Atractylodes Rhizome extract;spleen deficiency syndrome;gastrointestinal function;signal transduction
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shuyu capsule on μ-opioid receptors (MOR) expression in the hypothalamus of premenstrual syndrome (PMS) with liver-qi stagnation model rats, and to investigate its possible mechanism. Method: Rats were selected by using an open field test and vaginal smear in this experiment. The selected rats were divided into 4 groups:the normal group, the model group, the Shuyu capsule group(0.408 g·kg-1) and the naloxone group(0.4 mg·kg-1). The PMS with liver-qi stagnation model in rats was prepared by a chronic bondage stress method. The distribution and protein level of MOR in hypothalamus of rats were observed by immunofluorescence chemical technology and Western blot. Result: Compared with the normal group, the scores of open field test decreased, the distribution of messy-arranged MOR in hypothalamus increased, MOR protein expression increased in the model group (P<0.01). Compared with the model group, the scores of open field test increased, the distribution of messy-arranged MOR in hypothalamus decreased, MOR protein expression decreased in the Shuyu capsule and naloxone groups (P<0.01). Conclusion: Shuyu capsule could improve the symptoms of PMS with liver-qi depression syndrome, which may be achieved by regulating the MOR in the hypothalamus. In addition, the up-regulation of MOR in the central nervous system may be one of the pathophysiologic mechanisms.关键词：Shuyu capsule;premenstrual syndrome with liver-qi depression syndrome;μ-opioid receptors;Hypothalamus
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects and mechanisms of serum containing Rougan decoction on expression of osteopontin in human hepatic stellate cells induced by TGF-β1. Method: Thirty rats were randomly divided into 5 groups:the control group, the 3/5 d groups with low-dose Rougan decoction (9 g·kg-1), the 3/5 d groups with high-dose Rougan decoction (18 g·kg-1) of 6 rats each. Rats in each group received distilled water or Rougan decoction at 10 mL·kg-1·d-1 and the corresponding serums containing drug were obtained. Human hepatic stellate cell line LX-2 was cultured in DMEM containing 10% control serum or serum containing Rougan decoction. The proliferation of LX-2 cells was detected by using MTT method. LX-2 cells were pretreated with control serum or serum containing Rougan decoction for 24 h followed by incubation with TGF-β1 at the final concentration of 10 μg·L-1 for 24 h. The expression of OPN was detected by real-time PCR and Western blot, the expression of p-PKB was detected by Western blot. Result: Compared with the control group, the proliferation of LX-2 cells in serum containing Rougan decoction groups was significantly inhibited (P<0.05, P<0.01). Meanwhile, the expression of OPN and p-PKB were significantly down-regulated in serum containing Rougan decoction groups (P<0.05, P<0.01). Conclusion: The serum containing Rougan decoction has an inhibitory effect on the expression of OPN induced by TGF-β1, the mechanism may be related to the inhibition of PI3K/PKB signaling pathway.关键词：Rougan decoction;osteopontin;hepatic stellate cells;transforming growth factor-β1
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of plumbagin on the mRNA and protein expressions of α-smooth muscle actin (α-SMA) in human hepatic stellate cell (HSC )- LX2 cells stimulated by transforming growth factor-β1 (TGF-β1) in vitro. Method: HSC-LX2 cells were cultured in vitro and divided into the blank group, the model group, the high-, medium-and low-dose plumbagin groups (2.0, 1.5, 1.0 μmol·L-1). After being incubated with each drug for 72 hours, the mRNA expression of α-SMA was assayed by RT-PCR and the protein expression of α-SMA was assayed by immunocytochemistry. Result: Compared with blank group, the mRNA and protein expressions of α-SMA in HSC-LX2 cells increased in the model group (P<0.01). Compared with model group, the mRNA and protein expressions of α-SMA were inhibited in the high-and medium-dose plumbagin groups (P<0.05, P<0.01). Meanwhile, there were better results in the high-dose plumbagin group (P<0.01). Conclusion: Plumbagin could inhibit the activation and proliferation of HSC-LX2 cells and have certain anti-hepatic fibrosis effect.The mechanism may be related to restraining the mRNA and protein expressions of α-SMA.关键词：plumbagin;transforming growth factor-β1;HSC-LX2;α-smooth muscle actin;anti-hepatic fibrosis
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-hyperglycemic effect of the decoction of Rubus crataegiflolius roots in diabetic rats induced by alloxan. Method: Diabetic rat models were copied by iv alloxan through tail vein, then rats were randomly divided into 4 groups;the model group (distilled water, 13.5 mL·kg-1), the positive control group (metformin hydrochloride,210 mg·kg-1), the low and high dose groups (decoction of R. crataegifloliums roots, 1.35, 2.70 g·kg-1) of 10 rats each. Another 10 healthy rats were assigned to the normal group (distilled water, 13.5 mL·kg-1). The corresponding medicines were intragastrically administrated to rats once daily for 13 days. Changes of fasting blood-glucose (FBG), nitrogen oxide (NO), malonaldehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected. The organ coefficient was calculated. Result: Compared with the normal group, the levels of FBG, SOD and GSH-Px increased, the levels of NO and MDA decreased in the model group (P<0.05). Compared with model group, the levels of FBG and GSH-Px decreased in the low and high dose groups (P<0.05), the NO level increased in the low dose group (P<0.05), the MDA levels increased in the high dose group (P<0.05), and the SOD level decreased in the high dose group (P<0.05). Moreover, the organ damage could be repaired by R. crataegifldiums roots decoction. Conclusion: R. crataegiflolius roots decoction could decrease blood glucose by improving the anti-oxidation function of the body and the functional recovery of the isle.关键词：Rubus crataegiflolius roots decoction;metformin hydrochloride;diabete;Nitrogen monoxide;malondialdehyde;superoxide dismutase;glutathione peroxidase
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of rutin on renal function and pathological changes in diabetic nephropathy rats. Method: The model of diabetes nephropathy in rats was made by intraperitoneally injecting alloxan at 70 mg·kg-1 once weekly for 3 times continuously. The model rats were randomly divided into the model group, the high-, low-dose rutin groups (100, 200 mg·kg-1) and the irbesartan group (20 mg·kg-1). The healthy rats were assigned to the normal group. The corresponding medicines were intragastrically administrated to rats once daily for 12 weeks. Levels of fasting blood glucose (FBG), serum creatinine (SCr), blood urea nitrogen (BUN) in serum, 24-h urine protein and kidney index were tested. The renal pathological changes were examined by HE staining, Masson staining and electron microscopy. Result: Compared with the normal group, the levels of FBG, SCr, BUN, 24-h urine protein and kidney index all increased (P<0.01), kidney tissues such as glomerulus atrophy, renal capsule enlargement, renal interstitial area appearing much blue collagenous fiber, basilar membrane thicken diffusely, and fusion of foot process were injured remarkably in the model group. Compared with model group, rutin could decrease the levels of SCr, BUN, 24-h urine protein and kidney index (P<0.05, P<0.01). Conclusion: Rutin could improve the kidney function and reduce the renal injury degree in diabetic rats.关键词：rutin;diabetic nephropathy;kidney function;pathomorphology
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of epigallocatechin gallate (EGCG) on right ventricular hypertrophy induced by monocrotaline (MCT) in rats and explore its mechanisms. Method: Forty male Wistar rats were randomly divided into the normal group, the model group, the captopril group (25 mg·kg-1) and the EGCG (40 mg·kg-1) group. MCT-induced right ventricular hypertrophy model in rats was established, and the effects on right ventricular hypertrophy index (RVHI), right ventricular myocardial infarction (RVMI), nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β) and IL-6 of right ventricular hypertrophy myocardium tissue and right ventricular myocardial cells in rats were examined. Result: Compared with the normal group, RVHI and RVMI were significantly elevated (P<0.05),and NO, iNOS, IL-6 and IL-1β level of right ventricular hypertrophy myocardium tissue increased (P<0.05) in the model group. Compared with the model group, RVHI and RVMI was obviously declined (P<0.05), while levels of NO, iNOS, IL-6 and IL-1β significantly decreased (P<0.05) in the EGCG group. Conclusion: EGCG could significantly inhibit the RVH induced by MCT in rats. Its mechanisms may be related to reducing the levels of iNOS, NO, IL-1β and IL-6 in right ventricular hypertrophy myocardium tissue.关键词：epigallocatechin gallate;right ventricular hypertrophy;monocrotaline
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects and mechanisms of Xinjiangtang granule on kidney in streptozotocin-induced type 2 diabetic mellitus (T2DM) rats. Method: Fifty male SD rats were randomly divided into the normal control group (8 rats, normal diet) and the model group (42 rats). Rats in the model group received a high-fat-diet for 4 weeks and peritoneal injection of streptozotocin (STZ, 40 mg·kg-1) to induce T2DM. The T2DM rats were randomly subdivided into the model group, the metformin group (0.15 g·kg-1), the high-and low-dose Xinjiangtang granule groups (12.64, 6.32 g·kg-1). The corresoponding medicines were intragastrically administered to rats once daily for 8 weeks. After 8-week treatment, body weight, fasting blood glucose (FBG), ureanitrogen (BUN), serum creatinine (SCr), total cholesterol (TC), triglyceride(TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) of serum, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), catalase (CAT) and transforming growth factor-β1(TGF-β1) mRNA, plasminogen activator inhibitor-1(PAI-1) mRNA of kidney were detected. Result: Compared with the normal group, the levels of FBG, BUN, SCr, TC, TG, LDL-C, MDA and mRNA expressions of PAI-1,TGF-β1 increased, the body weight, levels of HDL-C, SOD, GSH-Px and CAT decreased in the model group (P<0.05, P<0.01). Compared with model group, the levels of FBG, BUN, SCr, TC, TG, LDL-C, MDA and mRNA expressions of PAI-1,TGF-β1 decreased, the body weight, levels of HDL-C, SOD, GSH-Px and CAT increased in the Xinjiangtang granule groups (P<0.05, P<0.01). Conclusion: Xinjiangtang granule could remarkably regulate glycometabolism of T2DM rats, and mechanism may be associated with inhibiting the mRNA expressions of TGF-β1 and PAI-l, weakening oxidative stress and improving kidney function.关键词：Xinjiangtang granule;diabetic nephropathy;oxidative stress;plasminogen activator inhibitor-1;transforming growth factor-β1
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Longmu Guizhi decoction(LD) on the α2A-AR mRNA expression in the left and right brain of juvenile spontaneously hypertensive rat(SHR),a rat model of attention deficit hyperactivity disorder(ADHD),and to investigate the mechanisms of LD for ADHD. Method: Male SHRs were randomly divided into high-dose LD group(18 g·kg-1), low-dose LD group(9 g·kg-1), methylphenidate group(3.75 mg·kg-1)and model group(saline, 10 mL·kg-1). All the SHRs were lavaged for 14 days. The α2A-AR mRNA expression in the left and right prefrontal cortex, corpus striatum were detected by the method of Real-time PCR. Result: In the right prefrontal cortex, α2A-AR mRNA expression was significantly decreased in the methylphenidate group and two LD groups compared with model group(P<0.05),but there was no obvious change in the left prefrontal cortex in all the groups. In the right corpus striatum, compared with saline control group,α2A-AR mRNA expression was significantly decreased in the methylphenidate control group, but it was significantly increased in the two LD groups. In the left corpus striatum, α2A-AR mRNA expression was significantly increased in the methylphenidate control group(P<0.05), there was no obvious change in other groups. Conclusion: The produce of ADHD may have a certain correlation with α2A-AR.The potential mechanism of LD in the treatment of ADHD is related to that it can decrease α2A-AR mRNA expression in the right prefrontal cortex,and increase its expression in the rigtht corpus striatum.关键词：Longmu Guizhi decoction;attention-deficit/hyperactivity disorder;left and right frontal-striatum;α2A adrenergic receptor
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effects and mechanism of Lianhua Qingwen (LHQW) capsule on lung tissue injury and expression of inflammatory cytokines in mice exposed to automobile exhaust. Method: Seventy-five healthy SPF male KM mice were randomly divided into 5 groups:normal group, model group, LHQW 2,4,8 g·kg-1 groups, and exposed to automobile exhaust in homemade cabinet in a noncontinuous way. The experimental animals were euthanatized 24 h later from the last exposure. The blood was used to detect the positive expression ratio of interleukin(IL) -4, IL-6 with flow cytometry. The serum of other animals was adopted to detect the content of IL-12 with ELISA. The pathological changes in lung tissue were determined with hematoxylin and eosin(HE) staining. Western-blot and real-time quantitative PCR were used to analyze the protein and mRNA expression of tumor necrosis factor-α(TNF-α), IL-1β, IL-4, IL-6, IL-12 and IL-13 in lung tissue. Result: The visible bronchial epithelium swelling, epithelial cell proliferation, necrosis, alveolar hemorrhage and exudation, perivascular edema could be seen in model group, the injury of lung tissue in LHQW 4, 8 g·kg-1 group was significantly reduced. The positive expression ratio of IL-6 was 20.1%, 17.6%, 11.4% in mice blood of LHQW 2,4,8 g·kg-1 group respectively, obviously lower than 32.9% in model group. Compared with normal group, the protein and mRNA expression of TNF-α, IL-1β, IL-4, IL-6, IL-12, IL-13 were significantly increased in the lung tissue of model group, and LHQW at the dose of 2, 4, 8 g·kg-1 could inhibit the expression at different extent. Conclusion: LHQW capsule could reduce the lung damage in mice exposed to automobile exhaust through reducing the content of inflammatory cytokines in the blood and lung tissues.关键词：Lianhua Qingwen capsule;automobile exhaust;tissue injury;inflammatory cytokines
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发布时间：2024-01-04 - 摘要：Objective: To discuss effect of extract compound preparation of Nelumbinis Folium,Herba seu Radix Gynostemmatis Pentaphylli and Perillae Folium on reducing of body weight and secrum lipid. Method: Male SD rats were randomly divided into 5 groups according to body weight,including the normal group,the model group,extract compound preparation in low,middle and high dosage groups.Rat body weight,serum lipids,fat mass and ratio of fat mass and body weight were investigated. Result: Compared with the model group,rat body weight of three extract compound preparation groups increased slowly with a significant change;fat mass was smaller with a significant change;total cholesterol(TC),serum truglyceride(TG),low density lipoprotein-cholesterol(LDL-C) were significantly decreased in three experimental groups;Lee's index of three experimental groups had a significant change;high density lipoprotein-cholesterol(HDL-C) of three experimental groups were higher than the model group. Conclusion: This extract compound preparation has a good anti-obesity anti-hyperlipemia effectiveness.关键词：Nelumbinis Folium;Herba seu Radix Gynostemmatis Pentaphylli;Perillae Folium;extract;obesity;hyperlipemia
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发布时间：2024-01-04 - 摘要：Objective: To explore effect of Eucommla ulmoides leaves flavonoids on blood glucose control and the protection of islet cells in diabetic rats. Method: Streptozocin was used to copy diabetic rats model,modeling rats were divided into four groups, model group, positive drug group(glyburide, 10 mg·kg-1),E.ulmoides leaves flavonoids groups (2.5,5.0 g·kg-1), the normal group was setup. After 28 days fasting blood glucose (FBG) was collected,serum free insulin (FINS)level and oral glucose tolerance test were carried out. Superoxide dismutase (SOD),glutathione peroxidase (GSH-Px)and malondialdehyde(MDA)content of pancreas homogenate was assayed. Hematoxylin and eosin (HE) staining was used to the microscopic observation islet morphology and cell number. Result: Compared with normal group,the significant increase of FBG and MDA,FINS falling and reduced glucose tolerance,the SOD and GSH-Px levels in model group rats were observed. Pathological islet apparently showed atrophy(P<0.01). Compared with model group, E.ulmoides leaves flavonoids could obviously decrease the MDA level,raise the level of SOD and GSH-Px,reduce FBG,improve glucose tolerance and the level of FINS(P<0.01, P<0.05). Pathological section showed that the rat islet was increased,the number of cells was increased obviously(P<0.01,P<0.05). Conclusion: E.ulmoides leaves flavonoids can obviously reduce the blood sugar of diabetic rats,improve the glucose tolerance,and increase the level of FINS,has the protection of islet cell function,antioxidative stress reaction is one of its mechanisms.关键词：Eucommla ulmoides leaves;flavonoids;diabetic rats;islet cell;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect and mechanism of Astragalic Radix extract on chronic nonbacterial prostatitis in rats. Method: Sixty SD rats were randomly divided into 6 groups:the normal group (distilled water), the model group (distilled water), the cernilton group (100 mg·kg-1), the low-, medium-and high-dose Astragalic Radix extract groups (100, 200 and 400 mg·kg-1) of 10 rats each. The chronic nonbacterial prostatitis model was prepared in rats except those in the normal group by injecting carrageenan. The corresponding medicines were intragastrically administered to rats once daily for 3 weeks. The effects on levels of prostatic index (PI), prostate-specific antigen (PSA), tumor necrosis factor-α (TNF-α), interleukin-1β(IL-1β), cyclooxygenase-2 (COX-2), prostaglandin E2 (PEG2), transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) were determined. The pathological changes of prostate tissue in rats were observed. Result: Compared with the normal group, the levels of PI, PSA, TNF-α, IL-1β, COX-2, PEG2, TGF-β1 and CTGF increased in the model group (P<0.01). Moreover, the pathological results showed obvious inflammatory cell infiltration and fibrosis in the model group. Compared with model group, the levels of PI, PSA, TNF-α, IL-1β, COX-2, PEG2, TGF-β1 and CTGF decreased in the Astragalic Radix extract groups (P<0.01). Meanwhile, the pathological morphology had good improvement. Conclusion: This study confirmed Astragalic Radix extract had therapeutically effective effect on chronic nonbacterial prostatitis in rats.关键词：Astragalic Radix;chronic nonbacterial prostatitis;inflammatory factor;pathological morphology
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to observe the influence of Danlou tablets on blood lipids,C-reactive protein(CRP),nuclear factor-κB(NF-κB) in patients with athemsclerosis(AS), and discuss the effect and mechanism involved in the process of anti-AS. Method: In this study, eighty-seven cases were divided into control group and Danlou group (treatment group) randomly, with control group treated with rosuvastatin merely, while treatment group by Danlou tablets and in the addition of rosuvastatin. After the treatment for 12 weeks, total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low density lipoprotein (LDL), lipoprotein a (LPa), apolipoprotein a (ApoA), apolipoprotein B (ApoB) in plasma were detected by automatic biochemical analyzer;CRP,NF-κB in peripheral blood were detected by the way of latex-enhanced immunoturbidimetric test and ELISA;carotid artery intima-media thickness (IMT) was detected by Philips ie33 ultrasound machine and plaque score of the AS was collected. Result: After the treatment, the level of TC,TG,LDL-C,LPa,ApoB in blood decreased, HDL in plasma rised, with significant difference (P<0.05,P<0.01) before and after treatment. TC,LDL-C,ApoB in plasma of the treatment group dropped obliviously, and there was significant difference compared with control group (P<0.05).It can be induced that it was more effective to combine conventional treatment with Danlou tablets than conventional treatment alone. Expression of CRP,NF-κB in treatment group reduced significantly, and there was significant difference compared with control group(P<0.01). According to the carotid intima-media thickness and plaque score displayed, AS plaque in treatment group declined significantly with significant difference(P<0.05). Conclusion: DanLou tablets could regulate the blood lipid levels effectively, and intervene in the carotid plaque progression. And one of the mechanisms of anti-AS is to lower the levels of CRP, NF-κB and other inflammatory cytokine.关键词：Danlou tablets;athemsclerosis;C-reactive protein;nuclear factor-κB
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to evaluate the effect of Liangxue Zishen Yiqi method on the level of hepatitis B virus-DNA(HBV-DNA)in dendritic cells (DCs), and on the peripheral lymphoid T cell in patients with chronic hepatitis B, in order to study the role of Chinese medicine method in the immune function, inhibition and removal of the virus in patients, and provide the new ideas for prevention and treatment of hepatitis. Method: In this study, one hundred and fifty-eight cases of HBV patients were randomly divided into treatment group and control group. The conventional treatment was applied in the two groups, Liangxue Zishen Yiqi medicine was given in treatment group while Liangxue Zishen Yiqi placebo prescription was given in control group. All the traditional Chinese medicine were granular formulation. Result: After the treatment for 24 weeks, the liver function, HBV-DNA, dendritic cells and proliferation of peripheral lymphoid T cell subsets were obviously improved, with no significant difference in the improvement of liver function between two groups. The reduction of HBV-DNA after treatment in control group was significantly different with control group (P<0.05),but with no significant difference in negative conversion ratio. The ratio of HBeAg negative conversion in treatment group was superior to the control group, however, the HBeAg sero conversion ratio was not. The levels of DCs molecules, and the secretion of DCs IL-12 levels were both superior to the control group (P<0.01,P<0.05);as to the proliferation of T lymphocyte subsets, it mainly improved the level of CD4+, and reduced the level of CD8+, with significant difference compared with control group (P<0.05). Conclusion: Liangxue Zishen Yiqi has positive effect on DC, lymphocyte subsets in patients with chronic hepatitis B. It can significantly restrain HBV-DNA, which may play a role in improving the immune function and arousing the T lymphocyte immune status of patients.关键词：Liangxue Zishen Yiqi method;dendritic cells;chronic hepatitis B;hepatitis B virus-DNA
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical efficacy of Tongmai Wendan decoction in patients with carotid artery atherosclerosis. Method: One hundred and twenty patients according to inclusion criteria were randomly divided into treatment group and control group, patients from treatment group receive Tongmai Wendan decoction and atorvastatin,and patients from control group was given atorvastatin,and the treatment period was 3 months, intima-media thickness of carotid arteries(IMT), blood lipid and symptoms before and after treatment were observed. Result: After treatment, IMT and blood lipid were improved in both groups, difference of IMT between the two groups was not statistically significant,difference of total cholesterol (TC)between the two groups was statistically significant(P<0.05),difference of low density lipoprotein cholesterol(LDL-C) between the two groups was statistically significant(P<0.05),difference of high density lipoprotein cholesterol(HDL-C) between the two groups was statistically significant(P<0.05),difference of trigly-ceride(TG) between the two groups was not statistically significant,some clinical symptoms such as dizziness, full feeling of the chest and sticky and greasy in mouth were improved in treatment group, the difference was statistically significant(P<0.05). Conclusion: Tongmai Wendan decoction can regulate the level of blood lipid in patients with carotid artery atherosclerosis and improve the symptoms of patients.关键词：Tongmai Wendan decoction;carotid artery atherosclerosis;intima-media thickness of carotid arteries;blood lipid
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to probe into the application value of the combination of Chinese medicine cycle treatment and letrozole for patients with ovulation disorder infertility in Shenxu Xueyu type. Method: The ninty-two patients with ovulatory disorder infertility in Shenxu Xueyu type were divided into treatment group and control group by digital form randomly. There were 46 cases in each group. The patients in the control group were treated with letrozole drug therapy. The patients in the treatment group were treated with letrozole combined with Chinese medicine cycle treatment. The clinical treatment of the patients in two groups were compared and analyzed. Result: Compared with control group, in treatment group, the recovery rate was 50.00% (23/46) and the total efficacy was 91.30%(42/46) with significant increase;after treatment, the primary follicular diameter was (19.89±3.25) mm, the endometrial thickness was (9.72±1.86) mm, both increased significantly;the ovulation rate was 76.09%, pregnancy rate was 45.65% and live birth rate was 90.48%, all increased significantly;while the abortion rate was 9.52%, which decreased significantly;after treatment, the levels of estradiol(E2) was (240.26±58.72) pmol·L-1, follicle-stimulating hormone(FSH) was (16.23±4.25) U·L-1, which both increased significantly, while the level of testosterone(T) was (1.69±0.30) nmol·L-1, luteinizing hormine(LH) was (24.35±6.18) U·L-1, LH/FSH was (1.51±0.73), which all decreased significantly;after treatment, the scores of TCM syndrome was (5.36±1.20) points, which reduced significantly;the ratio of TCM syndromes cure was 54.35% (25/46), the total efficiency was 95.65% (44/46), with both increased significantly. The differences were significant (P < 0.05). Conclusion: Chinese medicine cycle treatment combined with letrozole has a positive role in promoting the clinical effects and improving the clinical symptoms for patients with ovulation disorder infertility in Shenxu Xueyu type. It could serve as the ideal clinical treatment solution for patients with ovulation disorder infertility in Shenxu Xueyu type.关键词：Chinese medicine cycle treatment;letrozole;Shenxu Xueyu type;ovulation disorder infertility
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发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to study the effect of Houpu Wenzhong decoction combined with domperidone on plasma gastrointestinal hormone and curative effect on functional dyspepsia. Method: One hundred and fifteen cases with functional dyspepsia were divided into observation group (58 cases) and control group (57 cases), with control group given domperidone and observation group treated with domperidone and Houpu Wenzhong decoction. Efficacy, clinical symptoms scores, plasma gastrointestinal hormone level, and adverse drug reactions were compared between two groups. Result: Curative efficacy in observation group was significantly higher than that in control group (94.83% VS 80.70%);clinical symptom scores were all significantly lower than those in control group;in observation group, plasma motilin level was significantly higher than in control group [(279.31±60.47) VS (224.54±57.62) ng·L-1], and the plasma somatostatin in observation group was significantly lower than in control group[(9.38±4.14)VS(12.53±3.79) ng·L-1];there was no obvious adverse drug reactions occurred in both two groups. Conclusion: Houpu Wenzhong decoction combined with domperidone can help to improve plasma motilin and somatostatin levels, relieve the clinical symptoms, and improve the clinical curative effect.关键词：functional dyspepsia;Houpu Wenzhong decoction;domperidone;plasma motilin
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Wenyang Qufeng Huoxue method in treatment of incipient diabetic peripheral vascular disease. Method: One hundred and fourteen cases of patients were randomly divided into two groups, the control group of 51 cases was given conventional treatment of Western medicine while the treatment group of 62 cases was given Chinese medicine of Wenyang Qufeng Huoxue method on basis of the control group. After 3 months of treatment, to observe the efficacy and glycosylated hemoglobin (HbA1c), lipids [total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C)], hemorheology and other indicators. Result: The total effective rate of the treatment group was 96.8% while the control group was 80.4%, the two groups have significant difference (P<0.05);after treatment, the indicators except the HbA1c of the treatment group had a significant difference (P<0.05), HDL-C, hemorheology and brachial-ankle pulse wave veloc(baPWV) in the control group were significant difference with before, and the rest showed no significant difference;after treatment, the indexes except the HbA1c between the treatment group and the control group were significantly differences;there was no significant difference on adverse reaction between the two groups. Conclusion: Wenyang Qufeng Huoxue method is effective in treatment of incipient diabetic peripheral vascular disease that is worthy of promotion.关键词：diabetes;early peripheral vascular disease;Wenyang Qufeng Huoxue method
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Jiangtang Tongmai capsules in treating macroangiopathy of type 2 diabetes mellitus, and to investigate its influence on inflammatory response and microcirculation. Method: One hundred and forty-four patients were randomly divided into the control group (71 cases) and the observation group (73 cases) by a random number table. Patients in the control group received conventional glucose and blood pressure control, and took 40 mg simvastatin tablets and 100 mg aspirin enteric-coated tablets once daily. On the basis of the control group, patients in the observation group added 3 Jiangtang Tongmai capsules thrice daily. All patients in both two groups received 4 months of treatment. Before and after treatment, the carotid intermedium thickness and size of plaque were detected by color Doppler ultrasound. Levels of fasting blood-glucose(FBG), 2-hour postprandial blood glucose (PBG), glycated serum protein (GSP), tumor necrosis factor-α (TNF-α), high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), adiponectin (APN), fibrinogen (FIB), platelet maximum aggregation rate (MPAR) and D-dimer(D-D) were detected. Result: After treatment, cases of thicken of carotid intermedium thickness and size of plaque in the observation group were lower, carotid intermedium thickness and size of plaque were smaller than those in the control group (P<0.01). Level of GSP in the observation group was lower than that in the control group (P<0.01), and there was no statistically significant difference for the levels of FBG and PBG between two groups. Levels of TNF-α, hs-CRP, IL-6 D-D, FIB and MPAR in the observation group were lower (P<0.01), and level of APN was higher than those in the control group (P<0.01). Conclusion: Jiangtang Tongmai capsules could reduce the incidence rate of macroangiopathy of type 2 diabetes mellitus, and its mechanism of action may be related to reducing the symptoms of low rank inflammatory reaction and ameliorating microcirculation.关键词：type 2 diabetes mellitus;macroangiopathy;Jiangtang Tongmai capsules;inflammatory factor;microcirculation
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative efficacy of Juanxiao tablets for assistance in treating episodes of bronchial asthma of hot asthma syndrome, and to investigate its influence on levels of interleukin IL-6, IL-8 and IL-10. Method: One hundred and twenty-five patients were randomly divided into the control group (61 cases) and the observation group (64 cases) by a random number table. Patients in the control group received 1-2 spray salbutamol aerosols, and once every 4-8 h when necessary. For heavy patients, Budesonide nasal spray was added, 2 sprays to nostrils at morning and evening. Based on the treatment of the control group, patients in the observation group added 8 Juanxiao tablets thrice daily. All patients in both two groups received 2 weeks of treatment. The main symptoms including breathing, chest tightness, cough up phlegm, coughing, wheezing sound and asthma control questionnaire (ACQ) were graded before and after treatment. Lung function indexes such as forced expiratory volume in one second (FEV1), forced expiratory volume in first second (FEV1/FVC) and forced vital capacity ratio, peak expiratory flow (PEF), peak expiratory flow rate prediction percentage (PEF%pred) were evaluated. Levels of IL-6, IL-8, IL-10 and peripheral blood eosinophil (EOS) were tested. Result: The clinical efficacy of total effective rate in the observation group was 95.31%, which was superior to that of 81.97% in the control group (P<0.05). After therapy, scores of breathing, chest tightness, cough up phlegm, coughing, wheezing sound in the observation group were lower than those in the control group (P<0.01). The FEV1%pred, FEV1/FVC, PEF, PEF%pred in the observation group were higher than those in the control group (P<0.01). After treatment, levels of IL-6 and IL-8 in the observation group were lower, while IL-10 level was higher than those in the control group (P<0.01). The EOS and ACQ scores in the observation group were inferior to those in the control group (P<0.01). Conclusion: Juanxiao tablets could reduce clinical symptoms, improve lung function and control asthma in treating episode of bronchial asthma at (hot asthma syndrome). Its mechanism may be related to modulating inflammatory factor and reducing airway inflammation.关键词：bronchial asthma;hot asthma;Juanxiao tablets;inflammatory response;eosinophils
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Shuangu Sanzi capsules combined Bushen Jiangu capsules in treating osteoportic fracture, and to investigate its influence on alkaline phosphatase (ALP), bone gla protein (BGP) and bone morphogenetic protein (BNP-2). Method: One hundred and eighteen patients were randomly divided into the control group (58 cases) and the observation group (60 cases). Patients in the control group received 0.6 g calcium carbonate D3 chewable tablet every day. For female patients during menopause, 10 mg alendronate sodium tablets were added every day. Based on the treatment of the control group, patients in the observation group added 3 Shuangu Sanzi capsules and 4 Bushen Jiangu capsules thrice daily. All patients in both two groups received 6 months of treatment. Time of fracture healing and growth situation of bone callus was recorded. Before and after treatment, pain of waist and back was evaluated. The density of femur, tibia and levels of serous ALP, BGP and BMP-2 were detected. Result: The clinical effect of fracture healing in the observation group was superior to that in the control group (P<0.05). After treatment, density of the femur and tibia was higher (P<0.01), scores of pain of the waist and back were lower in the observation group than those in the control group (P<0.01). After 2 months of treatment, score of growth situation of bone callus was higher (P<0.01), the average time of fracture healing was lower in the observation group than those in the control group (P<0.01). Level of serous ALP was lower (P<0.01), and levels of BGP and BMP-2 were higher in the observation group than those in the control group (P<0.01). Conclusion: Shuangu Sanzi capsules combined Bushen Jiangu capsules could promote the healing of fracture, reduce the time of fracture healing, increase bone mineral density, alleviate pain and regulate levels of ALP, BGP and BMP-2 in treating osteoportic fracture.关键词：treating osteoportic;fracture;Shuangu Sanzi capsules;Bushen Jiangu capsules
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Yiqi Tongluo decoction combined thermal silver needle treatment instrument in treating postherpetic neuralgia (PHN) in middle-aged and elderly patients, and to investigate its influence on T cell subpopulations and serous β-endorphin (β-EP). Method: One hundred and twenty-five patients were randomly divided into the control group (61 cases) and the observation group (64 cases). Patients in the control group received 0.5 mg mecobalamin injection for intramuscular thrice weekly, 25 mg doxepin hydrochloride tablets twice daily, 1 grain of ibuprofen sustained release capsule twice daily. Patients in the observation group received one dose Yiqi Tongluo decoction combined thermal silver needle treatment instrument. All patients in both two groups received 8 weeks of treatment. Pain situation was evaluated by visual analogue scale (VAS). Before and after treatment, scores of Hamilton's depression scale (HAMD), Athens insomnia scale (AIS), qi deficiency and blood stasis and the quality of life index of skin disease (QLQI) were made. Levels of T cell subpopulations (CD3+, CD4+, CD8+, CD4+/CD8+) and β-EP were detected. Result: The clinical effect in the observation group was better than that in the control group (P<0.05). At the fourth and eighth week after treatment, scores of VAS in the observation group were lower than those in the control group (P<0.01). After treatment, scores of HAMD, AIS, qi deficiency and blood stasis and QLQI in the observation group were lower than those in the control group (P<0.01). Levels of CD3+, CD4+and CD4+/CD8+were higher (P<0.01), level of CD8+ was lower (P<0.01), and level of β-EP was higher (P<0.01) than those in the control group. Conclusion: Yiqi Tongluo decoction combined thermal silver needle treatment instrument could relieve the pain, ameliorate depression, insomnia and qi deficiency and blood stasis, improve patients' quality of life, regulate T cell subpopulations and improve the level of β-EP in treating middle-aged and elderly patients with PHN.关键词：postherpetic neuralgia;Yiqi Tongluo decoction;thermal silver needle treatment instrument;T cell subpopulations;serous β-endorphin
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effect of retention enema of Yuchang decoction combined mesalazine in treating ulcerative colitis (UC) of large intestinal dampness-heat syndrome, and to investigate its influence on interleukin (IL) -17 and IL-21. Method: One hundred and twenty patients with UC were randomly divided into the control group and the combined group of 60 patients each. The patients in the control group received 1 g mesalazine enteric-coated tablets quartic daily. Based on the treatment of the control group, patients in the combined group added retention enema of Yuchang decoction combined with mesalazine once daily. The patients in two groups received a 4-week period of treatment. Before and after treatment, colonoscopy was performed and the examinations were assessed by Baron scoring method and Sutherland DAI. Before and after treatment, the main signs and symptoms were scored. The quality of life in inflammatory bowel disease (IBD) was evaluated by IBD questionnaire (IBDQ). The levels of serum IL-17, IL-21 were detected. Result: The total effective rate in the combined group was 90%, which was superior to that of 76.67% in the control group (P<0.05). Baron scores and Sutherland DAI scores of the combined group were lower than those in the control group (P<0.01). The scores of diarrhea, bloody purulent stool, stomachache, and abdominal distension in the combined group were lower than those in the control group (P<0.01). IBDQ scores including intestinal tract symptom, constitutional symptom, emotional capability, and social competence of the combined group were higher than those of the control group (P<0.01). After treatment, the levels of serum IL-17 and IL-21 of two groups decreased (P<0.01), while the results were lower in the combined group (P<0.05). Conclusion: Using retention enema of Yuchang decoction combined with mesalazine could alleviate symptom and control disease activity in treating UC of large intestinal dampness-heat syndrome. Its effect is better than using mesalazine alone. The mechanism may be related to regulating inflammatory factors.关键词：ulcerative colitis;Yuchang decoction;retention enema;interleukin-17;interleukin-21
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Xiaoliu pills in treating uterus myoma of qi stagnation and blood stasis syndrome, and to explore its mechanism of action. Method: One hundred and ten patients were randomly divided into the control group (55 cases) and the observation group (55 cases) by a random number table. Patients in the control group received 25 mg mifepristone once daily. Patients in the observation group received 9 g Xiaoliu pills twice daily. All patients received 12 weeks of treatment. Before and after treatment, volume of fibroid and uterine was detected. Scores of qi stagnation and blood stasis were graded. Levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estrogen (E2), progesterone (P) and indexes of hemorrheology were detected. Result: The total clinical efficiency in the observation group was 94.55%, which was superior to that of 78.18% in the control group (P<0.05). After therapy, volume of fibroid and uterine in the observation group was smaller than that in the control group (P<0.01). At the eighth and twelfth week after treatment, scores of qi stagnation and blood stasis in the observation group were lower (P<0.01), falling range was larger than those in the control group (P<0.01). After therapy, levels of whole blood viscosity (high shear, low shear), plasmatic viscosity, fibrinogen and erythro-agglutation index decreased, and the results were lower in the control group (P<0.01). Levels of FSH, LH and E2 in observation group were lower than those in the control group (P<0.01), while, there was no statistically significant difference for the levels of PRL and P between two groups. Conclusion: The clinical effect of Xiaoliu pills on reducing volume of fibroid and uterine and decreasing scores of qi stagnation and blood stasis was superior to mifepristone. Moreover, it could ameliorate hemorrheology and regulate female sex hormone, so it is worthy to be used in clinic.关键词：myoma of uterus;qi stagnation and blood stasis;Xiaoliu pills;hormone;hemorrheology
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to explain the composing principles of the prescriptions for viral pneumonia collected in CNKI by traditional Chinese medicine (TCM) inheritance system. Method: The prescriptions used for viral pneumonia in CNKI were collected then input into the TCM inheritance system. TCM inheritance system was used to explain the composing principles of the prescriptions for viral pneumonia. Result: The composing principles were extracted based on the analyses of 173 cases of prescriptions by using TCM inheritance system.The frenquency of herbs were analyzed. The main association rule was Maxing Shigan Tang. There were 18 core combinations and 9 new prescriptions extracted. Conclusion: The complex composing principles of prescriptions for viral pneumonia collected in CNKI have been clarified by TCM inheritance system. The TCM inheritance system is very helpful to study the treatment for viral pneumonia by TCM.关键词：TCM inheritance system;viral pneumonia;composition principles
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to evaluate the clinical efficacy and safety of Simiao San as the main prescription for gouty arthritis. Method: CBM, CNKI, VIP database, Wanfang database, PubMed and EMbase were searched by computer for randomized controlled trials of Simiao San as the main prescription for gouty arthritis compared with western medicine. The quality of literatures was evaluated by using Cochrance system and Meta-analysis was performed by using Revman (version 5.2). Result: A total of 12 trials involving 937 patients were included. The Meta-analysis showed that the efficiency of Simiao San as the main prescription for gouty arthritis were better than using western medicine with [OR=2.28, 95%CI(1.54,3.37)].The rate of adverse drug reaction using Simiao San as the main prescription was less than using western medicine with [OR=0.12,95%CI(0.07,0.23)]. Conclusion: Compared with western medicine, Simiao San as the main prescription for gouty arthritis showed higher efficiency and safety.关键词：Simiao San;gouty arthritis;systematic evaluation;Meta-analysis
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发布时间：2024-01-04 - 摘要：We searched the database to consult the literatures on arrhythmia to obtain a summary about diagnosis and treatment of arrhythmia. There were mainly seven kinds of treatment of arrhythmia. The first is to combine with the specific disease in modern medical research, mainly after myocardial infarction, viral myocarditis, coronary heart disease, dilated cardiomyopathy, cor pulmonale, depression, high blood pressure, heart disease and acute coronary syndrome and heart failure combined quickness arrhythmia. The second is, according to the modern pharmacological study, to distinguish the single drug treatment, referring to the representative, and most studied traditional Chinese medicine(TCM):Sophorae Flavescentis Radix, Ginseng Radix et Rhizoma, Salviae Miltiorrhizae Radix et Rhizoma, Chuanxiong Rhizoma, Corydalis Rhizoma, Aconiti Lateralis Radix Preparata, Nardostachyos Radix et Rhizoma, Coptidis Rhizoma, and Ophiopogonis Radix. The third is to combine with modern preparation technology to develop static drops of TCM which is a widely used new dosage forms of TCM modernization. The fourth is, according to the TCM theory, four diagnostic and treatment methods based on syndrome differentiation are put forward. The fifth is bian gu fang differentiation with remarkable effect verified clinically. The sixth is bian acupuncture point treatment that is based on the theory of acupuncture, according to syndrome differentiation view in Chinese medicine, which is divided into acupuncture point acupuncture, acupuncture injection, acupuncture point bury line, and point massage. The seventh is to distinguish the ear differentiation. The integration of these seven methods, although it has low toxicity, low side effect, with global role in strengthening the body and improving the life quality of patients, could still lead to some defects. So the combination of Chinese medicine and western medicine, with increased research sample and objective index evaluation, can help to make the new breakthrough in the development of new drugs of TCM, and in the advance towards the world.关键词：overview;situation and prospect;quick arrhythmia
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发布时间：2024-01-04 - 摘要：Mori Folium is one of traditional Chinese medicines in China. Eight monomer alkaloids have been isolated from Mori Folium. Modern pharmacological studies have confirmed that these compounds have efficient activities of glucosidase inhibition, insulin secretion and inhibition of glycogenolysis. This article focused on the research progress of the monolithic structure, biological activity, extraction and purification technology and testing methods, by studying the chemical structure and properties of alkaloids, and analyzing its various content detection methods, to provide reference for the improvement of detection method of alkaloids. The current method for the determination of Mori Folium alkaloids focused on the study of its deoxynojirimycin(DNJ), relatively few studies on the total alkaloids content. On the basis of DNJ determination method, it is necessary to establish an appropriate method for the determination of total alkaloids Mori Folium, thus to provide a basis for the rational development and utilization of Mori Folium resources.关键词：Mori Folium;alkaloids;diabetes;determination
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发布时间：2024-01-04 - 摘要：Total flavonoids are main active components of epimedium and icariin is one of the most important active substances of epimedium total flavonoids, which has wide pharmacological effects such as anti-inflammation, anti-tumor, anti-osteoporosis, and cardio cerebral vascular protection, et al. In this paper, documents of epimedium total flavonoids and icariin about cardiovascular protection were collected from domestic and international literature database such as CNKI, PubMed and SpringerLink et al. The research progress mainly related to anti-atherosis, anti-hypertension, effects on hemodynamics and hemorrheology, anti-myocardial ischemia, anti-heart failure, we also collected data for cytokine and intracellular signaling pathways mechanisms and analyzed them at different depth. This paper has important theoretical value and practical significance for the new drug of epimedium total flavonoids and icariin and provides reliable literature information for the further studies.
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发布时间：2024-01-04 - 摘要：The chemical constituents, efficacy, in vivo process and related intellectual property rights on Abelmoschi Corolla were summarized. The main chemical structure types from Abelmoschi Corolla include myricetin, quercetin and gossypetin. It was mainly used for chronic kidney disease and wound infection, with the ability to suppress the immune response and anti-inflammatory effect. The metabolic processes in vivo involved in carboxyl reaction, acetylation reaction, hydrolysis reaction, and absorption by passive diffusion. Intellectual property rights concentrated on compund extraction, preparation and drug compatibility, lacking modern clinical and basic research.This paper reviewed progress on Abelmoschi Corollat, put forward the research idea.关键词：Abelmoschi Corolla;chemical compasition;pharmacological activity
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