最新刊期
卷 21 ， 期 18 ， 2015
- 摘要：Objective: To investigate influence of calcining temperature, calcining time and vinegar-quenching times on phase transformation of Pyritum and clarify variation rule and processing mechanism. Method: X-ray diffraction was adopted to qualitative and quantitative evaluate phase composition of different Pyritum sample. Result: Calcining temperature, calcining time and vinegar-quenching times all had a certain impacts on phase transformation in processing process of Pyritum, but processing temperature showed the most obvious effect, then quenching times affected phase transformation of Fe2O3 more obvious than processing time.Phase transformation rule of Pyritum was decomposed from FeS2 to Fe7S8 at first, the main phase of samples that processed under 600 ℃ was Fe7S8, whereas the main phase of Pyritum that processed under 600 ℃ calcined for 4 hours and qunched 3 times was Fe2O3.Along with calcining temperature rised to 800 ℃, the phase was mainly oxidized from Fe7S8 to Fe2O3. Conclusion: Calcining temperature, calcining time and quenching times all have different level of effect on phase composition of Pyritum, and the phase transformation observes some rules.关键词：pyritum;calcining temperature;calcining time;vinegar-quenching times;X-ray powder diffraction spectrum;phase variation
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发布时间：2024-01-04 - 摘要：Objective: To compare micromeritic properties and dissolution of each ultrafine powder of berberine hydrochloride for improving its dissolution efficiency. Method: Berberine hydrochloride was crushed by vibration mill.Particle size, specific surface area and porosity of samples were detected.The crystal structure of ultrafine powder was analyzed by differential scanning calorimetry (DSC).In vitro dissolution of berberine hydrochloride power was investigated. Result: After crushed, the minium value of d0.9 (90% of particle size cumulative distribution) of berberine hydrochloride could reach 38.78 μm.In a word, specific surface area and porosity varied from grinding time, they significantly increased in 4 minutes;after 4 minute, they decreased with increasing of grinding time.DSC curve indicated that the product of crystallinity decreased in different extent.Dissolution test showed that dissolution effect of berberine hydrochloride powder improved by comparing with the original powder, dissolution effect of the 4 min powder by crushing was the best. Conclusion: After crushed by vibration mill, some powder properties and dissolution of berberine hydrochloride can improve, this study will give a theoretical guidance for clinical medication and formulation development of drugs.关键词：micronization;berberine hydrochloride;micromeritic property;dissolution;specific surface area;porosity
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method for in vitro dissolution of lactones in Xianglian Wan, and reveal its dissloution behavior and key influencing factors. Method: Small cup method and HPLC were used to determine dissolution quantity of costunolide and dehydrocostuslactone from Xianglian Wan under different pH, rotational speed of stirring paddle and tween-80 concentration, cumulative dissolution rate was calculated and dissloution curves were drawn.Origin Pro 8.0 was used to fit dissolution curves with model and calculate dissolution parameters. Result: Under all pH dissolvents and rotational speeds of stirring paddle, cumulative dissolution rate of costunolide and dehydrocostuslactone in 6 hours were less than 50%.When tween-80 concentration exceeding 0.3%, cumulative dissolution rate of these two components in 4 hours both exceeded 90%.With increasing of tween-80 concentration, both T50(the time for dissolution 50%) and Td (the time for dissolution 63.2%) of these two components obviously became shorter, their dissolution speeds became quicker. Conclusion: In vitro dissolution of lactones in Xianglian Wan are not completely, the concentration of surfactant in dissolution medium has significant influence on dissolution of lactones in Xianglian Wan.关键词：Xianglian Wan;costunolide;dehydrocostuslactone;dissolution
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of Yuanhu Zhitong soft capsules about removing tannins and enriching alkaloids. Method: Gallic acid was indicators to purify tannins, tetrahydropalmatine was indicators to purify alkaloids.Single factor test was adopted to optimize purification process of removing gallic acid and enriching tetrahydropalmatine in Yuanhu Zhitong soft capsules. Result: AB-8 macroporous resin was selected, its best process of enriching tetrahydropalmatine was:the concentration of sample solution of 0.28 g· mL-1, diameter height ratio of 1:10, the largest adsorption capacity of 1.366 mg for per gram of resin, eluent of 95% ethanol, elution flow rate of 2 BV· h-1, eluent amount of 5 BV.Under these conditions, adsorption rate was 84.7%, elution ratio was 85.0%.Purity of total alkaloids in the final product was up to 3.63%. Conclusion: AB-8 resin can be used to remove tannins and enrich alkaloids, this study provides a reference for improving clinical efficacy and patient compliance of Yuanhu Zhitong soft capsules.关键词：Yuanhu Zhitong soft capsules;AB-8 macroporous resin;tetrahydropalmatine;gallic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of Qiangzhi Yishen capsules with macroporous resin. Method: Absorption ratio and elution rate of tenuifolin, paeoniflorin, glycyrrhizic acid and liquiritin were taken as indexes to screen out types of macroporous resins.Effects of the concentration of solution, diameter-height ratio and eluent volume on purification process of Qiangzhi Yishen capsules were investigated by single factor tests. Result: HPD-100 macroporous resin was selected, optimum purification process was as following:the mass concentration of sample loading solution 0.1 g· mL-1, resin-material drug (3:1), diameter-height ratio of resin 1:7.5, sample loading speed 3 BV· h-1, decontaminated with 4 BV of water, and then eluted with 5 BV of 70% ethanol, elution speed of 3 BV· h-1.Transport rates of tenuifolin, paeoniflorin, glycyrrhizic acid and liquiritin were 78.55%, 88.62%, 82.47%, 83.15%, with rate of extract of 7.52% and drying time of 0.5 h. Conclusion: This process can effectively remove starch, saccharides and other impurities in water extract, these purified substances is easy to use in preparations.关键词：Qiangzhi Yishen capsules;macroporous resin;tenuifolin;paeoniflorin;glycyrrhizic acid;liquiritin
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发布时间：2024-01-04 - 摘要：Objective: To study individual biomass of vegetative organs and alkaloids content in rhizome of Coptidis Rhizoma under different growth condition, and provide basic research data of optimal conditions for this herb in Emei county. Method: The weighing method and RP-HPLC was applied to determined biomass and compare contents of alkaloids in Coptidis Rhizoma under different altitude, shading way and cultivation density from Emei GAP base. Result: At an altitude of 1 200-1 800 m in Emei county, individual biomass of Coptidis Rhizoma had no significant difference, and contents of seven kinds of alkaloids conformed to requirement of the 2010 edition of Chinese Pharmacopoeia.Compared with traditional covering means, one and two layer shading net was no significant difference, but the new technology was saving labor and materials, protecting the forest resources.Production increased 32% per hectare with planting spacing of 10 cm×10 cm by comparing with 12 cm×12 cm. Conclusion: This study indicates that proper plant method, which is in altitude of 1 200-1 800 m, with monolayer net and planting spacing of 10 cm×10 cm, should be taken to improve quality and output of Coptidis Rhizoma.This method is proved to be practical for quality control of samples from GAP base of Coptidis Rhizoma.关键词：Coptidis Rhizoma;altitude;shading way;planting density;biomass;alkaloids
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发布时间：2024-01-04 -
Screening and Amelioration of DGGE Analysis Conditions for Endophytic Bacteria of Chuanxiong Rhizoma
摘要：Objective: To screen available 16S rDNA sequences of endophytic bacteria sampled from Chuanxiong Rhizoma for further denaturing gradient gel electrophoresis (DGGE) analysis, and optimize suitable analysis conditions. Method: In order to get DNA sequences of endophytic bacteria gathered from Chuanxiong Rhizoma, polymerase chain reaction (PCR) and nested PCR experiments have been conducted to the V3 region and the V6-V8 region of 16S rDNA respectively.Vertical electrophoresis has been used to ameliorate degeneration gradient of gels, time interval method has been used to figure out electrophoresis time, in addition, some modification of voltage-time context finally been made according to electrophoretograms. Result: The 16S rDNA V6-V8 region was ideal for DGGE analysis, owing to that it's effective to purify DNA of endophytic bacteria from their host of Chuanxiong Rhizoma, as a result makes it easy to get diverse sequences.These conclusive ideal analysis conditions for this region:gradient range of denaturing agent 55%-70% (Gel 6%), electrophoresis temperature at 60 ℃, voltage of 50 V, electrophoresis time of 16 h. Conclusion: These DGGE analysis conditions can be useful for distinguishing 16S rDNA V6-V8 sequences of endophytic bacteria of Chuanxiong Rhizoma.This research lays a reliable foundation for further study of exploring endophytic bacterium community structure of Chuanxiong Rhizoma, as well as many other medicinal plants.关键词：Chuanxiong Rhizoma;endophytic bacterrium;denaturing gradient gel electrophoresis;polymerase chain reaction;primer- 4
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发布时间：2024-01-04 - 摘要：Objective: To compare contents of five main effective components (aucubin, geniposidic acid, chlorogenic acid, catechin and rutin) in Eucommiae Folium from 17 different areas. Method: HPLC was employed to determine contents of five main effective components with mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution and detection wavelength at 208 nm.Contents of five main effective components in Eucommiae Folium from 17 different areas were investigated. Result: There were significant differences between contents and total content of these five main effective components in Eucommiae Folium from different areas.The highest total content of five effective components was 14.15% which was from Huangling county, Shaanxi province;samples with the lowest total content (1.37%) of them was from Quxian county, Sichuan province;difference of these two values was more than 10-fold. Conclusion: There are some regional differences of contents of five main effective components in Eucommiae Folium. The total content of main effective components in samples from Shaanxi and Henan province is more than from southwest area of our country, such as Sichuan, Guizhou province and Chongqing city.Eucommiae Folium should be possible to take low temperature rapid drying.关键词：Eucommiae Folium;aucubin;geniposidic acid;chlorogenic acid;catechin;rutin
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of ten lignans in Schisandrae Chinensis Fructus, and analyze difference of contents of ten lignans in the pulp, seed shell and seed kernel of Schisandrae Chinensis Fructus for researching regularities of their distribution. Method: HPLC was developed for determination of schisandrol A, gomisin D, gomisin J, schisandrol B, angeloylgomisin H, gomisin G, schisantherin A, schisandrin A, schisandrin B and schisandrin C in Schisandrae Chinensis Fructus from different area, mobile phase consisted of acetonitrile-water for gradient elution, detection wavelength was set at 220 nm and injection volume was 4 μL. Result: The content of total lignans in the seed kernel, seed shell and pulp of Schisandrae Chinensis Fructus from different area were 30.08, 9.76, 2.24 mg· g-1, respectively.Contents of ten lignans in these three parts of Schisandrae Chinensis Fructus showed significant difference, which decreased in the order of the seed kernel> the seed shell> the pulp. Conclusion: The developed method displays distribution information of lignans in three parts of Schisandrae Chinensis Fructus well, which can provide a reference for clinical application of Schisandrae Chinensis Fructus.关键词：Schisandrae Chinensis Fructus;lignans;distribution;gomisin D;gomisin J;schisandrol B
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发布时间：2024-01-04 - 摘要：Objective: To develop a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of five components (gastrodin, militarine, loroglossin, arbutin, 4-hydroxybenzenemethanol) in Gymnadeniae Conpseae Rhizoma, in order to provide basis for the quality control. Method: Gastrodin was used as the internal reference substance, and the relative correlation factors (RCF) with militarine, loroglossin, arbutin and 4-hydroxybenzenemethanol were determined by HPLC-UV. The contents of the four components were calculated according to the RCF, respectively. The contents of these components in 10 batches of Gymnadeniae Conpseae Rhizoma were determined by the external standard method. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Result: RCF of gastrodin-arbutin, gastrodin-4-hydroxybenzenemethanol, gastrodin-militarine and gastrodinloroglossin were 3.083 1(2.0%), 1.562 2(2.1%), 1.545 0(1.7%), 1.742 5(1.9%), respectively. For the five components, there was no significant difference between the calculated and determined values RSD(<3%)of the 10 batches by the two different methods. Conclusion: The method established in this study is accurate and feasible and can be used for the determination of five components in Gymnadeniae Conpseae Rhizoma simultaneously.关键词：Gymnadeniae Conpseae Rhizoma;quantitative analysis of multi-components by single-marker;relative correlation factors;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop a RP-HPLC method for the simultaneous determination of 16 amino acids in Xianling Gubao capsule. Method: With norleucine as the internal standard, hydrolyzed amino acids of Xianling Gubao capsule were derivatized with phenylisothiocyanate (PITC) and determined by RP-HPLC. Hypersil C18 column (4.6 mm×250 mm, 5 μm) was eluted with mobile phase A acetonitrile-0.1 mol· L-1 sodium acetate solution-acetic acid (7:93:0.05) and mobile phase B acetonitrile-water (80:20) in a gradient mode at a flow rate of 1 mL· min-1. The detection wavelength was set at 254 nm and the column temperature was 40 ℃. Result: The 16 amino acids were separated within 33 min with a good linearity (r>0.996 8) within the range of the test concentration. The average recoveries ranged between 97.5% and 100.7% and the RSD values were less than 5%. Conclusion: The method is simple, quick, accurate and reliable and can be used for the determination of amino acids in Xianling Gubao capsule.关键词：Xianling Gubao capsule;pre-column derivation;amino acids;phenylisothiocyanate;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize HPLC methods of loganin, paeonol and verbascoside, we detected the content of three components in Liuwei Dihuang Wan that prepared by sulfur-fumigated Dioscoreae Rhizoma, in order to reveal the effect of sulfur-fumigation on Traditional Chinese Medicine formulae. Method: The column was a Thermo Hypersil BDS C18(4.6 mm×250 mm, 5 μm). Loganin was gradiently eluted by acetonitrile-0.1% phosphoric acid with water system as mobile phase, and 236 nm as the detection wavelength. Paeonol was isocraticly eluted by acetonitrile-0.1% phosphoric acid with water (50:50), and 274 nm as the wavelength. Verbascoside was gradiently eluted by acetonitrile-0.1% phosphoric acid with water system as mobile phase, and 236 nm as the wavelength. The column temperature was 30 ℃, the flow rate was 1 mL· min-1, the injection volume was 10 μL. Result: With the increase of SO2 amount, loganin, paeonol and verbascoside derived from Liuwei Dihuang Wan were obviously decreased. In thesodium sulfite group, the content of the three components was the lowest, which to 0.022 6, 0.691 2 and 0.036 2 mg· g-1. Conclusion: The optimized HPLC methods had good accuracy, sensitivity and repeatability, which was suitable for determining in Liuwei Dihuang Wan. The results indicated that sulfur-fumigated Dioscoreae Rhizoma would have an adverse effect on Liuwei Dihuang Wan;the possible reason came from SO2 residue.关键词：Dioscoreae Rhizoma;sulfur-fumigation;Liuwei Dihuang Wan;loganin;paeonol;verbascoside
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发布时间：2024-01-04 -
Determination of Esculin and Esculetin in Cortex Fraxini by Cloud Point Extraction Coupled with HPLC
摘要：Objective: A new method was developed for the determination of esculin and esculetin in Cortex Fraxini by cloud point extraction coupled with HPLC. Method: The surfactant Genapol X-080 was chosen as the extract solvent. Some affecting factors such as the concentration of surfactant, liquid/solid ratio, the concentration of sodium chloride, equilibration temperature and equilibration time were investigated and optimized. Result: The optimum conditions were achieved of 0.18 g· mL-1 surfactant concentration, 100:1 liquid/solid ratio, 1.5 mol· L-1sodium chloride concentration for 35 min under 60 ℃. Conclusion: This methodology offers the advantages of safety, efficiency and easiness. Under the optimum conditions, the average recoveries of esculin and esculetin were 95.3%, 96.0% and RSD were 2.3%, 2.1%. The method was successfully applied to determine esculin and esculetin in Cortex Fraxini respectively.关键词：cloud point extraction;Cortex Fraxini;HPLC;determination- 3
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发布时间：2024-01-04 - 摘要：Objective: To study the Chemical Constituents of Uncariae Ramulus Cum Uncis. Method: The compounds were obtained and purified by column chromatography on silica gel column, Sephadex LH-20, ODS and preparative HPLC, the structures of these compounds were identified according to their physicochemical properties and spectral data. Result: Fourteen compounds were obtained from the ethanol extract of Uncariae Ramulus Cum Uncis, their structures were elucidated as β-sitosterol (1), daucosterol (2), chlorogenicacid (3), corynoxine (4), corynoxeine (5), isocorynoxeine (6), rhynchophylline (7), isorhynchophylline (8), protopine (9), cadambine (10), 3α-dihydrocadambine (11), vincoside lactam (12), strictosamide (13), hyperoside (14). Conclusion: Compound 9 was obtained from this genus for the first time. Compounds 3, 13 and 14 were obtained from the plant for the first time.关键词：rubiaceae;Uncariae Ramulus Cum Uncis;indole alkaloids;strictosamide
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发布时间：2024-01-04 - 摘要：Nine compounds were isolated from the rhizome of Smilax hypoglauca by column chromatography on silica gel and recrystallization. The structures of the these isolated compounds were identified by spectroscopic analysis(MS, 1H-NMR, 13C-NMR, IR). These compounds were identified as β-sitosterol(1), epifredelinol(2), friedelin(3), 7-methyl-5, 3', 5'-trimethoxy-flavone(4), physcion(5), emodin(6), 5, 6, 4'-trihydroxy-7, 8-dimethoxy-flavone(7), β-daucosterol(8), linarin(9).All compounds were isolated from this plant for the first time.关键词：Smilax hypoglauca;Smilax;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method of determination of 1-deoxynojirimycin in Jintangning capsules. Method: The samples were collected after elution with ammonia by processed D001-CC type positive cation exchange resin and pre-column derivatization with the concentration of 2 g· L-1 of 9-fluorenylmethyl chloroformate (Fmoc-Cl) and then determinated by DAD detector. ODS-C18(4.0 mm×250 mm, 5 μm)was eluted with the mobile phase of acetonitrile and 0.2% phosphoric acid in a gradient mode. The flow rate was 1.0 mL· min-1, the detection wavelength was 263 nm, and column temperature at 25 ℃. Result: 1-deoxynojirimycin had good linear relationships within the range of 0.84-8.4 μg (r=1.000 0), with the precision RSD of 0.3% and repetition RSD of 0.6%, the sample solution after derivatization was stable within 24 hours. The average recovery was 109.6%, and the relative standard deviations were 0.4%. Conclusion: The method is stable, reliable and accurate, and can be used for the determination of 1-deoxynojirimycin in Jintangning capsules.关键词：1-deoxynojirimycin;Jintangning capsules;pre-column derivatization;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To screen out the active components from Corydlis Decumbentis Rhizoma (CDR) and investigate their inhibitory effect on β1-AR. Method: A high expression β1 adrenergic receptor/cell membrane chromatography-offline-ultra-performance liquid chromatography/mass spectrometry method (β1-AR/CMC-Offline-UPLC/MS method) was used to scan and identify the effective components from extracts of CDR. β1-AR/CHO-S cells were cultured in metoprolol (10 μmol· L-1) and active components of jatrorrhizine (JAT) (1, 5 μmol· L-1) for 24 h. Then, the cells were injured in DMEM containing ISO (50 μmol· L-1) for 10 h. the effect of the active components on β1-AR downstream factors cAMP and PKA was determined by ELISA kits to evaluate their inhibitory effect on β1-AR. Result: JAT was the active component of CDR and the experiment in vivo showed that in JAT and MT groups, concentrations of cAMP and PKA were lower than that in the ISO group (P<0.01). Conclusion: JAT was successfully isolated and identified by the β1-AR/CMC-offline-UPLC/MS system from CDR and had an inhibitory effect on β1-AR. The β1-AR/CMC-Offline-UPLC/MS method can be used to screen out active components with clear action targets in a quick and effective manner. The screening results were found to be significantly consistent with its pharmacological effect.关键词：β1 adrenergic receptor;cell membrane chromatography;active components;jatrorrhizine
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发布时间：2024-01-04 - 摘要：Objective: To compare the effect of processing on naphthopyrone glycosides and anthraquinone aglycones in Semen Cassiae. Method: The high-performance liquid chromatographic method was used to determine the content of three naphthopyrone glycosides and three anthraquinone aglycones in Semen Cassiae and compare the changes in the content before and after processing. Result: The content of three naphthopyrone glycosides significantly decreased after processing. Specifically, rubrofusaringentiobioside dropped about 21%, cassiaside decreased by 60%, cassiaside C decreased by 87%. As for the changes in the content of the three anthraquinones, there was no significant difference between aurantio-obtusin and chryso-obtusin, but obtusifolin increased by 48%. Conclusion: Processing has a significant effect on the content of naphthopyrone glycosides and anthraquinone aglycones in Semen Cassiae. Specifically, naphthopyrone glycosides in Semen Cassiae decreased integrally, whole anthraquinone aglycones increased partially after processing.关键词：Semen Cassiae;processing;naphthopyrone;anthraquinone;change in content
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for the simultaneous determination of aloe-emodin and physcion in Wikstroemia indica. Method: Diamonsil C18column (4.6 mm×250 mm, 5 μm) was eluted with the mobile phases of acetonitrile-0.10% phosphoric acid water in a gradient mode. The UV detection wavelength was 254 nm. The flow rate was 1.0 mL· min-1. The column temperature was set at 30 ℃, and the sample size was 20 μL. The difference in the content of aloe-emodin and physcion in W. indica from from ten origins was compared. Result: All of aloe-emodin and physcion samples showed a good linearity within the range of 1.584-25.34 mg·L-1(r=0.999 2), 1.598-25.57 mg· L-1(r=0.999 3), respectively. The average recoveries were 100.2% and 99.7%, with RSD of 1.8% and 1.1%, respectively. There was a great difference in the content of aloe-emodin and physcion in W. indica of different origins. Specifically, W. indica from Shaoguang, Guangdong province, had the highest content of aloe-emodin but the lowest content of physcion;W. indica from Fuzhou, Fujian province, had the highest content of physcion but the lowest content of aloe-emodin.Aloe-emodin was not detected in 120724 W. indica from Zhejiang, and physcion was not detected from Anshun, Guizhou province. Conclusion: The method is simple, rapid and accurate and so can be applied for the quality control of W. indica.关键词：Wikstroemia indica;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: To explorethe influence of different processing methods on contents of gallic acid, ellagic acid content according to the content of the two compounds in 10 batches of Galla Chinensis, vinegar gallnut and Chinese gall leaven, separately. Method: 10 batches of crude drugs of Galla Chinensis were collected. 10 batches of vinegar products and Chinese gall leaven were processed. The contents of thegallic and ellagic acid in different processed products were detected by RP-HPLC method.Kromasil C18 column (4.6 mm×150 mm, 5 μm) was used. Mobile phase A was acetonitrile, mobile phase B was 0.1% three fluoride acetic acid. Gradient elute was used. Flow rate was set at 0.8 mL·min-1. The detection wavelength was 280 nm, the column temperature was set at 30 ℃. Result: The content of gallic acid and ellagic in raw materials, vinegar, fermentation were 20.19, 1.73; 74.26, 4.59; 236.11, 0.59 mg· g-1. Conclusion: The content of gallic acid in Galla chin can be increased by vinegar method and fermentation method significantly(P<0.01), significantly. Fermentation method can raised the content of this component more significantly than vinegar method (P<0.01). The content of ellagic acid can be raised in vinegar-process pieces significantly(0.01< P< 0.05). The difference of the content of this compound between crude drug and fermentation pieces was not statistically significant.关键词：Galla Chinensis;processed piece;RP-HPLC;gallic acid;ellagic acid
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发布时间：2024-01-04 - 摘要：Objective: To detect serum metabolites in rats with chronic myocardial ischemia after intragastric administration of Guizhi decoction. Method: Rats were divided into 3 groups randomly, included the Guizhi group, the model group and the normal group.The normal group was given normal saline with dose of 0.04 mg· kg-1 once a day simultaneously, the other groups were intraperitoneal given isoprenaline hydrochloride with dose of 0.04 mg· kg-1 once a day, six days in a row, after modeling, the Guizhi group was intragastric administration of Guizhi decoction with dose of 0.625 g· mL-1, then after two weeks, HE staining of the heart tissue of rats was observed.Characteristics of serum metabolites in rats were measured by nuclear magnetic resonance mass spectrometry, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Result: Compared with the model group, rat cells of the Guizhi group was completed with neat muscle fiber and no edema.Rat serum samples of the Guizhi group were closely to the normal group, two samples were separated with model accurate parameter of Q2>0.9.Twenty-eight metabolites were detected in rat serum of three groups, including carbohydrate, amino acids, organic acids and so on. Conclusion: Guizhi decoction can reduce the degree of chronic myocardial injury.Metabonomics is an effective technique for detecting traditional Chinese medicine in the treatment of cardiovascular disease.关键词：Cinnamomi Ramulus;decoction;chronic myocardial ischemia;biomarkers;metabonomics;principal component analysis
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发布时间：2024-01-04 - 摘要：Objective: To compare effect of different compatibility of Platycodonis Radix and Achyranthis Bidentatae Radix on tissue distribution of paeoniflorin in normal and blood stasis mice, preliminary study leading action of Platycodonis Radix and Achyranthis Bidentatae Radix. Method: Normal and blood stasis mice were gavaged with Xuefu Zhuyu Tang, lacked Platycodonis Radix or Achyranthis Bidentatae Radix parties.Mice were killed after 30 minutes, whole blood collected, the brain, heart, liver, spleen, lung and kidney were removed.HPLC was employed to determine the content of paeoniflorin with mobile phase of acetonitrile-water (16:84), detection wavelength at 230 nm and geniposide as internal standard. Result: After normal mice being gavaged with Xuefu Zhuyu Tang full party, single lacked Platycodonis Radix party, single lacked Achyranthis Bidentatae Radix party, lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, the content of paeoniflorin in lung were 0.010 2, 0.005 8, 0.091 0, 0.037 3 μg· g-1, those in kidney were 0.122 2, 0.052 0, 0.144 0, 0.065 0 μg· g-1, but paeoniflorin were unchecked in the brain, heart, liver and spleen.After blood stasis mice being gavaged with Xuefu Zhuyu Tang full party, single lacked Platycodonis Radix party, the content of paeoniflorin in lung were 0.076 4, 0.042 0 μg· g-1, those in kidney were 0.335, 0.210 μg· g-1, but it was unchecked in the brain, heart, liver and spleen.After blood stasis mice being gavaged with Xuefu Zhuyu Tang single lacked Achyranthis Bidentatae Radix party, lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, the content of paeoniflorin in lung were 0.274 7, 0.019 7 μg· g-1, those in kidney were 0.467 1, 0.149 0 μg· g-1, it almost were unchecked in the brain, heart, liver and spleen. Conclusion: Making comparison based on Xuefu Zhuyu Tang lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, Platycodonis Radix can increase the content of paeoniflorin in the lung and kidney in normal mice, Platycodonis Radix can more significantly increase the content of paeoniflorin in the lung than that in the kidney in blood stasis mice.Achyranthis Bidentatae Radix can decrease the content of paeoniflorin in the lung and kidney in normal mice, it can increase the content of paeoniflorin in the lung and kidney in blood stasis mice, it also can make distribution of paeoniflorin in the heart, liver, spleen in blood stasis mice.This study confirms Platycodonis Radix and Achyranthis Bidentatae Radix have effect of leading action.Leading sites of Platycodonis Radix and Achyranthis Bidentatae Radix are not single.关键词：Xuefu Zhuyu Tang;Platycodonis Radix;Achyranthis Bidentatae Radix;paeoniflorin;leading action
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发布时间：2024-01-04 - 摘要：Objective: To develop a sensitive, rapid and effective LC-ESI-MS/MS method for investigating the uptake pathways and mechanisms of matrine in HepG2 cells. Method: Hanbon Megres RP-C18 column (4.6 mm×250 mm, 5μm) was adopted. The mobile phase was 10 mmol· L-1ammonium acetate solution (containing 0.1% formic acid)-methanol (35:65). The MS quantification was performed by ESI ion source at the positive ion mode and selective reaction monitoring (SRM). With HepG2 cells as the in vitro model, the effect of temperature, drug concentration and selective inhibitors of polyspecific organic cation transporters on the cellular uptake of matrine were investigated. Result: Matrine in HepG2 cell lysate showed a good linearity within the range of 0.05 and 50.0 nmol· L-1 and the lower limit of quantification was 0.05 nmol· L-1. The uptake at 4 ℃ was significantly lower (P<0.001) than that at 37 ℃. The uptake was significantly decreased(P<0.01) when the concentration was increased from 0.5 μmol· L-1 to 400 μmol· L-1. Furthermore, the uptake of matrine was significantly inhibited (P<0.001) by pyrilamine, verapamil, quinidine and diphenhydramine. Conclusion: A simple, sensitive and rapid LC-ESI-MS/MS method was developed to study the uptake mechanism of matrine in HepG2 cells. The uptake of matrine into HepG2 cells is an active transport mechanism, which is likely due to the organic cation-sensitive transport system.关键词：matrine;HepG2 cells;LC-ESI-MS/MS;cellular uptake
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发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-metastatic effects of ethyl acetate extracts from Callicarpa nudiflora (ECN) and its mechanisms. Method: BALB/c Nu mice were used to establish in vivo experimental metastasis mode and the mice were randomly divided into control group and ECN groups treated with 0.125, 0.25, 0.5 g· kg-1 ECN respectively. After 8 weeks of gavage (once a day), human cytokeratin 19(CK19) mRNA expression in lungs of mice was checked by nest PCR to determine effects of ECN on the formation of lung metastatic foci of breast cancer. In vitro invasion and migration assay were involved to determine effects of ECN on metastatic properties of breast cancer cells. MDA-MB-231 cells were treated using ECN at dose of 100, 200 mg· L-1 and BB-94 with the final concentration of 10 nmol· L-1(n=4). After 14 h incubation, the invaded and migrated cells were calculated. In vitro adhesion assay was also used in this study. MDA-MB-231 cells were treated using ECN at dose of 100, 200 mg· L-1and BB-94 with the final concentration of 10 nmol· L-1(n=3). After 60 min incubation, cells attached to matrix proteins were detected. p-Snail and E-cadherin expressions were checked by western blot method in current study to detect the effects of ECN on activation of Snail in MDA-MB-231 cells, which were treated with 100, 200 mg· L-1ECN for 24 h. Result: ECN induced a significant decrease on human CK19 mRNA expression in lung of tumor-bearing mice(P<0.01). Moreover, in vitro adhesion, migration and invasion of MDA-MB-231 cells were inhibited significantly when these cells incubated with ECN (P<0.01). It was also showed that ECN affected activity of Snail and expression of E-cadherin in breast cancer cells. Conclusion: ECN shows significant anti-metastatic properties. The mechanisms of action of ECN may be associated with suppression of Snail activation.关键词：Callicarpa nudiflora;metastatisis;breast cancer;Snail;E-cadherin
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发布时间：2024-01-04 - 摘要：Objective: To observe pharmacodynamic effects and mechanism of simplified Qingluo Tongbi prescription (SQLT). Method: The model of rheumatoid arthritis was induced by collagen Ⅱ in DBA/1 mice. The mice were randomly divided into blank group, collagen-induced arthritis (CIA) group, SQLT group (8.4 g· kg-1), original Qingluo Tongbi prescription (QLT) group (13 g· kg-1) and polyglucosides of Triplegium wilfordii (T Ⅱ) group (9 mg· kg-1). General conditions and joint swelling were observed. All groups were treated with corresponding drugs through the intragastric administration for four weeks. Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), anti collagen Ⅱ(CⅡ) antibody in serum were measured, and hematoxylin-eosin(HE) staining was conducted to observe the histopathologic changes and pathological score of ankle tissues. Result: Compared with the normal group, the model group showed significant joint swelling and increases in TNF-α, IL-1β and anti CⅡ antibody, with high ankle osteoarthritis scores (P<0.01).Compared with the CIA group, QLT, SQLT and T Ⅱ groups showed notable reductions in the paws swelling, arthritis scores, contents of IL-1β, TNF-α and anti CⅡ antibody in serum (P<0.01, P<0.05), and inhibition in the histological damage and cumulative arthritis injury scores. Conclusion: SQLT rationally integrates herbs with different properties, tastes and efficacies, with a similar effect and mechanism with the original prescription.关键词：Qingluo Tongbi prescription;prescription simplification;collagen-induced arthritis mice;efficacy
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effective hypoglycemic part in different extracts from Leontopodium leontopodioides and its dosage by determining blood glucose in hyperglycemia mice. Method: L. leontopodioides were extracted by 70% ethanol and the extracts were separated by D101 macroporous resin and then eluted successively with 50%, 70%, 95% of ethanol to obtain 50%, 70%, 95% of ethanol extracts. Hyperglycemia mice were induced by streptozotocin (STZ) and then perfused respectively with metformin hydrochloride (300 mg· kg-1), ethanol extracts of 50% (100 mg· kg-1), ethanol extracts of 70% (100 mg· kg-1) and ethanol extracts of 95% (100 mg· kg-1) after 7 days. The model and blank groups were perfused with the same amount of distilled water. The fasting blood glucose of hyperglycemia mice were measured after 10 days. The screened effective hypoglycemic parts were divided into high (400 mg· kg-1), medium (200 mg· kg-1) and low (100 mg· kg-1) dose and used to treat hyperglycemia mice;10 days later the fasting blood glucose was measured. Result: The model group showed a significantly higher blood glucose level than the blank group. Compared with the model group, different extracts of L. leontopodioides can reduce hyperglycemia mice blood glucose, the ethanol extracts of 50% had an obvious effect, and the medium dose was the best. Conclusion: The effective hypoglycemic part in L. leontopodioides was 50% ethanol extracts.关键词：Leontopodium leontopodioides;hyperglycemia mice;hypoglycemic activity;ethanol extracts
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发布时间：2024-01-04 - 摘要：Objective: To investigate protective effect of polysaccharide from Vitis viniferal (VTP) on expression of beta-amyloid precursor protein (APP) in PC12 cells induced by β-amyloid protein25-35(Aβ25-35), in order to discuss the mechanism of VTP in pathogenesis of Alzheimer's disease (AD). Method: Alzheimer's disease (AD) cell model was established by inducing PC12 cells with 20 μmol· L-1 Aβ25-35 for 24 h and intervened with different concentrations of VTP (20, 40, 80 mg· L-1). Cell viability was detected by CCK-8.The activities of dismutase superoxide(SOD) and malondialdehyde(MDA) were measured. The expression of APP mRNA was quantified by real-time PCR. Result: Compared with the normal group, the model group showed decreases in cell viability and SOD activity (P<0.01) and increases in MDA content and APP mRNA expression (P<0.01). Compared with the model group, cell viability and SOD activity were increased (P<0.05), MDA content and APP mRNA expression were decreased in VTP groups (P<0.05), indicating the relief in neuronal injury. Conclusion: VTP has a protective effect in the oxidative injury induced by Aβ25-35 and plays a role in preventing and treating AD by resisting oxidation, adjusting APP mRNA expression, inhibiting Aβ formation and proteting neurocytes.关键词：Alzheimer's disease;polysaccharide from Vitis viniferal;PC12 cells;oxidative damage;beta-amyloid precursor protein
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study is to investigate the effects of licochalcone A (LCA) on the invasion and metastasis in mouse melanoma B16F10 cells. Method: The cell proliferation rate was evaluated using sulforhodamine B(SRB) method. And the anti-metastasis ability was observed using scratch wound assay. The activated of matrix metal proteinase-2(MMP-2) and matrix metal proteinase-9(MMP-9) were evaluated using gelatin zymography. The expression in protein level of MMP-2 and MMP-9 were detected using enzyme-elinked iemmunosorbent essay (ELISA).The mRNA expression of MMP-2 and MMP-9 were assessed using quantitative real-time polymerase chain reaction (PCR) and reverse transcription-PCR. Result: There was no significant effect on proliferation rate after treated with LCA 5-15 μmol· L-1 in B16F10 cells. LCA attenuated B16F10 cells migration in a concentration-dependent manner. LCA was able to reduce the activities of MMP-2 and MMP-9, down-regulated the expression of MMP-2 and MMP-9 both in mRNA and protein levels. Conclusion: LCA attenuated mouse melanoma B16F10 cells migration by inhibition of MMP-2 and MMP-9 expression.关键词：licochalcone A;anti-metastasis;matrix metal proteinase;mouse melanoma B16F10 cells
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发布时间：2024-01-04 - 摘要：Objective: To investigated the effect of Huanglian Wendan Tang (HLWDT) on insulin resistance and neurogenesis in hippocampus of diabetic rats. Method: The combined treatment of high-glucose-high-fat diet for 50 days and injection of 35 mg· kg-1 streptozocin (STZ) was used to establish rat type 2 diabetic mellitus model. The successfully modeling rats were selected in the following trial based on blood glucose. Then, the modeling rats were allotted into 6 groups:model group, metformin (0.2 g· kg-1), berberine (0.2 g· kg-1), HLWDT (3, 6, 12 g· kg-1) respectively, and the drugs were given by gavage for 30 days except normal and model groups. Oral glucose tolerance test (OGGT) was performed. The release of insulin (Ins), expression of insulin receptors (InsR) and β-secretase (BACE1) mRNA were detected in hippocampus neuron. Finally, the number and morphology of neurons were observed by a hematoxylin and eosin (HE) staining of dentate gyrus (DG) region in hippocampus. Result: After treatment of high-glucose-high-fat diet and 35 mg· kg-1 STZ injection, model group rats showed a significantly impaired OGGT (P<0.01), higher expression of InsR (P<0.01), lower expression of BACE1 (P<0.05), compared to the normal rats. The decreased number of neurons in DG region of hippocampus in model group rats was observed. All these adverse changes were reversed by treatment of metformin (0.2 g·kg-1), berberine (0.2 g· kg-1), HLWDT (3, 6, 12 g· kg-1) (P<0.05, P<0.01). Conclusion: Treatment of HLWDT can improve the impaired OGGT and decreased expression of BACE1 mRNA in hippocampus of diabetic rats, as well as the impaired neurogenesis in DG region of hippocampus. The underlie mechanism may be related to increasing expression of InsR mRNA in hippocampus neuron.关键词：diabetes;insulin resistance;hippocampus;Huanglian Wendan Tang
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine on fracture healing, and define its effect in strengthening the healing of fracture and its possible mechanism. Method: Totally 160 male healthy New Zealand rabbits were divided randomly into blank control group (blank group for short), Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine group (combined therapy group for short), Jiegu Chuangshang ointment control group (treated only with Jiegu Chuangshang ointment, control group for short) and model group, with 40 rabbits in each group. Except for the blank group, all of the other animals were included in the right radius fracture model and were subdivided randomly into 4 small groups at 4 time points-the 2th, 4th, 6th, 8th weeks, with 10 in each group. The combined therapy group was treated with Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine, and control group only received the treat of Jiegu Chuangshang ointment, blank group and model group were not treated. At the 2th, 4th, 6th, 8th week, serum alkaline phosphatase (AKP) activity and bone gla protein (BGP) content were determined, and the bone mineral density test, X-ray imaging check, histological and morphological detection were completed. Result: Compared with the normal group, the model group showed significant lower serum AKP and BGP content, bone mineral density and X-ray imaging score (P<0.01) and obvious pathological and morphological detection results. Compared with the model group, at the 2th, 4th, 6th, 8th week after treatment, the activity level of serum AKP and BGP content of the combined therapy group was significantly higher (P<0.05, P<0.01). Besides, the bone mineral density, X-ray imaging score, histological sections and morphological detection also shows that the fracture healing in the combined therapy group was ahead of schedule (P<0.05, P<0.01), and the differences at each time point between the two groups were significant statistically (P<0.05, P<0.01). Conclusion: Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine accelerate the healing of fracture by influencing the whole process of fracture healing.关键词：Jiegu Chuangshang ointment;iontophoresis of traditional Chinese medicine;fracture healing;serum alkaline phosphatase;Bone Histomorphometry
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Kangxianling docotion on PI3K/AKT/mTOR signaling pathway and its mechanism against renal fibrosis. Method: Totally 60 C57 mice were randomly divided into sham group (n=10) and operation group (n=50), the surgical group received 5/6 nephrectomy. After two weeks, the surgery group were randomly divided into model group, Kangxianling low, medium and high dose groups and rapamycin positive control group, with 10 in each group. The sham group received 0.5 mL of normal saline, ig, Kangxianling docotion low, medium and high dose groups were given 0.5 mL Kangxianling drug orally (0.1, 0.2, 0.4 mg· kg-1), rapamycin group was given 0.5 mL rapamycin gavage (0.016 μg· kg-1). Mice were sacrificed after 12 weeks of gavage. The 24 h urine protein before the mice were sacrificed to detect 24 h protein quantification, the orbital blood was collected to test serum creatinine, blood urea nitrogen;the remnant kidney was collected to observe renal morphology by HE change;PCR was used to detect PI3K/AKT/mTOR mRNA expressions in kidney tissues. Result: Compared with sham operation group, model group showed significantly higher 24-hour urinary protein excretion, serum creatinine, blood urea nitrogen, PI3K/AKT/mTOR mRNA expressions (P<0.01), with significantly renal pathological changes. Compared with the model group, all treatment groups showed lower 24-hour urinary protein excretion, serum creatinine, blood urea nitrogen, PI3K/AKT/mTOR mRNA expressions (P<0.05), with improvements in renal histology. Conclusion: Kangxianling can decrease 24 h urinary protein quantification in mice, improve renal function and delay the occurrence of renal fibrosis;its mechanism may be related to the inhibition of the expression pathway PI3K/AKT/mTOR signal.关键词：Kangxianling docotion;5/6 nephrectomy;PI3K/AKT/mTOR signaling pathway;renal fibrosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Shenkang injection (SKI) on the morphology changes induced by peritoneal dialysis solution (PDS) as well as tumor necrosis factor-α(TNF-α) and transforming growth factor-β1 (TGF-β1) in peritoneal dialysis rats. Method: Fifty SD rats were randomly divided into normal group, 4.25%PDS group, 4.25%SKI low, middle and high dose groups. Except for the normal group, all of the remaining four groups were intraperitoneally injected with low, middle and high doses of SKI+4.25%PDS. At the 8th week after the injection, rats were anesthetized with chloral hydrate, and the peritoneal solution was gathered. The level of TNF-α was detected by ELISA method. The visceral peritoneum tissues of rats were stained with hematoxylin-eosin (HE) to observe the changes of peritoneal morphology. Immunohistochemistry were used to analyze the expression of TGF-β1. Result: Compared with the normal group, rats in the PDS group showed significantly higher TNF-α and expressions of TGF-β1 (P<0.01), swellon and deciduous peritoneal mesothelial cells, thickened sub-mesothelial matrix, and severe vascular proliferation and inflammatory cell infiltration. Compared with the PDS group, SKI middle and high dose groups showed decrease in TNF-α content (P<0.01). all SKI groups showed great reliefs in the structure changes of the peritoneum, decrease in the inflammatory cell infiltration and expression of TGF-β1 (P<0.05, P<0.01). Conclusion: SKI may be helpful in ameliorating the peritoneal fibrosis induced by PDS, which may be correlated with inhibiting increases in TNF-α and TNF-β1.关键词：Shenkang injection;peritoneal fibrosis;tumor necrosis factor-α;transforming growth factor-β1
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Erxian Yikun decoction on the ability of learning and memory of perimenopausal female mice. Method: The female mice was ovariectomized to simulate perimenopausal status and then randomly divided into model group, diethylstilbestrol group (DES, 0.1 mg· kg-1), and Erxian Yikun decoction high, middle land low dose groups(30, 15, 7.5 g· kg-1). The sham operation group was also established as the control group. The mice were given corresponding drugs for 5 weeks. The effect on the ability of learning and memory in the different groups was investigated by using step down test and Morris water-maze test. The superoxide dismutase(SOD) and malondialdehyde(MDA) level in serum, lipid peroxidase(LPO), nitric oxide(NO) and acetyl choline(ACh) level in brain tissue, as well as the activity of SOD, nitric oxide synthase(NOS) and acetyl choline esterase(AChE) were also detected. Fluorescence quantitative PCR was used to detect Bcl-2 Assaciated x protein(Bax) and B cell lymphoma/lewkmia-2(Bcl-2) mRNA expressions in the hippocampus. Result: Compared with model group, the ability of learning and memory was significantly improved, the SOD activity in serum was increased, and the SOD activity and MDA level was decreased in the different treatment groups (P<0.05, P<0.01). Meanwhile, the ACh level in brain tissue was increased, the AChE activity and LPO level and NO level were also decreased in the treatment groups, and Erxian Yikun decoction activated the Bcl-2 and lowered expressions of Bax (P<0.05, P<0.01). Conclusion: Erxian Yikun decoction has the effect in improving the ability of learning and memory, this mechanism may relate to scavenging free radicals, anti-oxidization and regulating neurotransmitter level and influencing the effect of Bcl-2 and Bax expression.关键词：Erxian Yikun decoction;perimenopause;learning and memory
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and anti-influenza A virus (H1N1) mechanism of volatile oil from Cinnamomi Ramulus (VOCR) and its major component cinnamaldehyde in vivo. Method: Totally 70 KM mice were divided into seven groups:the control group, the model group, the Ribavirin group (0.15 g· kg-1), VOCR high and low dose groups (0.348, 0.174 mg· kg-1) and cinnamaldehyde high and low dose groups (0.264, 0.132 mg· kg-1), with 10 mice in each group. The model group was intratracheally injected with 50 μL H1N1 liquid to reproduce the H1N1 model and then continuously given drugs for 5 d to observe the inhibitory effect in the hemagglutination titer in lung tissues. The ELISA test was conducted to observe the effect on the secretions of serum interleukin-2(IL-2), IL-6 and tumor necrosis factor-α(TNF-α). Flow cytometry was used to detect the effect on T cell subsets after the five-day continuous treatment in the VOCR (0.348 mg· kg-1) group, the cinnamaldehyde (0.264 mg· kg-1) group, the control group, the model group and the Ribavirin (0.15 g· kg-1) group. Result: Compared with the normal group, the model group showed significant increases in the hemagglutination titer in lung tissues, mice serum IL-6 and TNF-α but notable decreases in CD4+/CD8+ and IL-2 content (P<0.05). Compared with the normal group, the VOCR (0.348 mg· kg-1) group and the cinnamaldehyde (0.264 mg· kg-1) group showed reduction in the serum levels of IL-6 and TNF-α separately (P<0.05);the Cinnamaldehyde (0.264 mg· kg-1) obviously increased the rate of CD4+ to CD8+ T cells (P<0.05). All groups show no remarkable effect on serum IL-2 content. Conclusion: VOCR and cinnamaldehyde could significantly decrease the hemagglutination titer in lung tissues of H1N1-infected mice and show a better anti-H1N1 effect. The mechanism of anti-influenza A virus effect s correlated with the regulation of the ratio between the secretions of serum cytokines IL-2, IL-6, TNF-α and the increase in T cell subsets of model mice.关键词：volatile oil from Cinnamomi Ramulus;cinnamaldehyde;influenza A virus A/PR/8/34;hemagglutination titer;cytokine;T cell subset
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发布时间：2024-01-04 - 摘要：Objective: To investigate the curative effect of Pogostemonis Herba oil in guinea pigs in the acute otitis media model. Method: Doubling dilution was used to determine the MICs (minimum inhibitory concentration) of Pogostemonis Herba oil against common pathogens in otitis media(Staphylococcus aureus, Moraxella catarrhalis and Pseudomonas aeruginosa). The acute otitis media model was established by puncturing tympanic membrane and injecting methicilln-resistant S. aureus(MRSA) at 1.0 McF· mL-1 into the middle ear. After the successful modeling, the guinea pigs were divided into six groups:Pogostemonis Herba high, middle and low dose groups (100, 50, 25 g· L-1), positive groups (Vancomycin 100 g· L-1 and Ofoxacin 5 g· L-1) and the model group. All of them were dropped with corresponding drugs through ears. The model group was given isopyknic normal saline, 0.04 mL a time, 2 times a day, for consecutively days. Their middle ear cavities were cleaned with sterilized saline water before the first, second, fourth, sixth, eighth administration. The cleaning liquors were diluted and used for counting bacterial colonies. At the seventh day after the administration, the guinea pigs were beheaded to collect middle ear cavities for HE staining and observe the inflammatory cell infiltration and distribution on middle ear mucosa. Result: Pogostemonis Herba showed a MIC value of 0.21 g· L-1 against S. aureus and 0.026 g· L-1 against M. catarrhalis, but with no effect against P. aeruginosa. Compared with the model group, Pogostemonis Herba oil can significantly reduce the bacteria number in middle ears of guinea pigs in the acute otitis media model (P<0.05) and the inflammatory cell infiltration at the middle ear mucosa. Conclusion: Pogostemonis Herba oil has a good efficacy in treating guinea pigs in the acute otitis media model.关键词：Pogostemonis Herba oil;staphylococcus aureus;acute otitis media
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发布时间：2024-01-04 - 摘要：Objective: To compare the effect of different flavonoids on a cell anti-inflammation, immunity and chondrocyte proliferation based on the decomposed formula of Yaotongning capsule (YTNC), in order to provide basis for screening candidate fractions treating rheumatoid bone diseases. Method: Five flavonoids:Glycyrrhiza uralensis (Fgu), Epimedium sagittatum (Fes), Carthamus tinctorius (Fct), Davallia mariesii (Fdm), Taxillus chinensis (Ftc), at 50% and their proportional compounds were mixed into alkaloids of Strychnos nux-vomica and Ephedra sinica to obtain six formulas. Their half effective inhibitory concentration (IC50) and half effective concentrations (EC50) were adopted to evaluate the effect of the samples in promoting interleukin-6(IL-6), IL-1β, tumor necrosis factor-α(TNF-α) inflammatory factor in macrophages Ana-1, inhibiting prostaglamd in E2(PGE2) secretion released from Ana-1 cells induced by LPS and promoting chondrocyte proliferation. The optimization method based on least squares was applied to calculate additive EC50/IC50 value of each sample. The interactions between the effective fractions were analzed by comparing the differences between the additive EC50/IC50 values with the corresponding experimental EC50/IC50 values. Result: The formula mainly consisting of Fdm had the best effect in promoting the secretion of IL-1β. The formula consisting of Fct had the best effect in stimulating IL-6 secretion, and other activities second only to the best formulas. The formula consisting of Strychnos nux-vomica, Ephedra sinica and the five flavonoids had the best effect among the inhibiting PGE2 secretion and promoting TNF-α secretion and chondrocyte proliferation, and a strong synergistic effect among the effective fractions in this formula was observed. In the model for promoting chondrocyte proliferation, there were strong antagonistic effects among effective fractions in the formula consisting of single flavonoid and alkaloid. Conclusion: There is excellent synergistic interaction among different kinds of flavonoids. Fct, Fdm and flavonoid mixtures have good synthetic pharmacological activities in anti-inflammation, immunomodulation and chondrocyte proliferation, suggesting that Fct, Fdm and flavonoid mixtures can be used to optimize candidate effective fractions of flavonoids for traditional Chinese medicines for treating rheumatism bone diseases.关键词：rheumatism bone disease;Yaotongning capsule;effective fractions of flavonoids;prostaglandin E2;immunomodulation;chondrocyte proliferation
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Sanhuang Huoluo formula on blood glucose, renal function, sterol regulating element binding protein-1c (SREBP-1c) and SREBP-2c of diabetic nephropathy rats. Method: The diabetic model was induced by streptozotocin. The rats in the successfully established diabetic model were randomly divided into the model group, the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups. After other groups took the resection of the right kidney, the positive drug group was given 1.3 mg· kg-1 of benazepril hydrochloride, Sanhuang adjustable low dose group, middle dose group and high dose group were orally given Chinese medicine granules with doses of 5, 15, 30 g· kg-1, respectively. Both of the normal group and the model group mice were orally given distilled water, and all of the groups orally given drugs for 8 weeks in a row. Blood glucose (GLU), glycosylated hemoglobin (HbA1c), blood urea nitrogen (BUN), creatinine (SCr), urine protein (Pro), SREBP-lc, SREBP-2c in rats were observed. Result: Compared with the normal group, GLU and HbA1c of the model group were significantly higher (P<0.01), GLU and HbA1c of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were also significantly higher (P<0.01). Compared with the model group, GLU of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were lower (P<0.01), HbA1c of the positive drug group and Sanhuang Huoluo formula medium and high doses groups were lower (P<0.01), HbA1c of the Sanhuang Huoluo formula low dose group was lower (P<0.05). Compared with the normal group, SCr, BUN, Pro, SREBP-lc and SREBP-2c of the model group were significantly higher (P<0.01), and SCr, BUN, Pro, SREBP-lc and SREBP-2c of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were also significantly higher (P<0.01). Compared with the model group, SCr, BUN, Pro, SREBP-lc and SREBP-2c of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were lower (P<0.01). Compared with the positive drug group, SCr, BUN, Pro, SREBP-lc and SREBP-2c of the Sanhuang Huoluo formula low dose group were higher (P<0.01), and SCr, BUN, Pro, SREBP-lc and SREBP-2c of the Sanhuang Huoluo formula medium dose group were higher (P<0.05). Conclusion: Sanhuang Huoluo formula can improve level of SCr, BUN, Pro, SREBP-lc and SREBP-2c in diabetes nephropathy rats, with a certain protective effect on renal damage in rats of diabetic nephropathy rats.关键词：diabetic nephropathy;Sanhuang Huoluo formula;renal function
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发布时间：2024-01-04 - 摘要：Objective: To investigate the curative efficacy of Yimucao injection combined with carboprost tromethamine in preventing cesarean section postpartum hemorrhage and on levels of fibrinogen(FIB) and D-dimer. Method: One hundred and forty cases of obstetric indications for cesarean section and with high risk factors of uterine inertia were divided into 2 groups equally according to random number table:the control group(n=70) and the observation group (n=70). After delivery of the fetus in cesarean section, the control group was treated with uterus injection of carboprost tromethamine, while the observation group was treated with intramuscular injection of Yimucao injection and uterus injection of carboprost tromethamine. Their 2, 24 h postoperative blood loss, the incidence of postpartum hemorrhage, duration of uterine contraction after the drug injection, lowering speed of uterine bottom after delivery, duration of lochia, level of FIB, D-dimer and adverse reactions were observed and compared. Result: In comparison with the control group, 2, 24 h postoperative blood loss were significantly lower (234.5±50.2) VS (356.2±51.5), (309.7±64.5) VS (443.3±68.6) mL and the incidence of postpartum hemorrhage was significantly lower (4.3% VS 14.3%) in the observation group (both P<0.05). After the drug injection, the observation group had a significantly longer duration of uterine contraction (3.43±1.03) VS (1.62±0.54) h, quicker lowering speed of uterine bottom (1.15±0.24) VS (0.56±0.13) cm· d-1 and shorter duration of lochia (14.8±4.7) VS (21.5±6.2) d than those in the control group (all P<0.05). And level of FIB (3.50±0.24) VS (4.61±0.42) g· L-1, D-dimer (1.21±0.23) VS (3.47±0.29) mg·L-1 in the observation group was respectively lower than that in the control group after the treatment (both P<0.05). During the treatment, there was no case of severe adverse reaction and no statistical difference in inter-group comparison of the incidence of adverse reactions (18.6% VS 21.4%). Conclusion: Therapy of Yimucao injection combined with carboprost tromethamine was effective in the prevention of cesarean section postpartum hemorrhage, and could effectively reduce postoperative blood loss, decrease the incidence of postpartum hemorrhage, lengthen duration of uterine contraction, promote postpartum recovery and mitigate high blood coagulation, with minor adverse reactions. It is worth further studies in clinic.关键词：Yimucao injection;carboprost tromethamine;cesarean section;postpartum hemorrhage;fibrinogen;D-dimer
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy of modified Yinchen Sini Tang in treating patients with acute-on-chronic liver failure (ACLF) characterized by Yin jaundice syndrome. Method: Two hundred and sixty eligible patients with ACLF characterized by Yin jaundice syndrome were randomly divided into the control group (n=130) and the treatment group (n=130). They were respectively given the simple comprehensive western therapy and the combined therapy with modified Yinchen Sini Tang for 8 weeks. Baseline characteristics were compared between both groups before and after the treatment.Before and after treatment, patients' symptoms and signs score, liver function [including alanine aminotransferase (ALT), total bilirubin (TBIL) and albumin-albumin (ALB)], primary trait analysis (PTA), model for end-stage liver disease(MELD) changes were recorded, and traditional Chinese medicine(TCM) syndromes, 8-week treatment effect and short-term (12 weeks) differences in prognosis of two groups were compared. Result: The treatment group was significantly superior to the control group in symptom scores (P<0.01). In the overall response rate of TCM symptoms, the treatment group was 87.90%, while the control group was 60.83%, with a significant difference (P<0.05). After treatment, both groups showed improvements in TBIL, ALT, ALB, PTA, MELD scores, the difference was significant (P<0.01). At the 8th week after treatment, the overall response rate in the treatment group were significantly higher than the control group (91.54% VS 53.85%) (P<0.01). At the 12th week, more patients were survived in the treatment group than the control group (P<0.05). Conclusion: Modified Yinchen Sini Tang can relieve clinical symptoms of patients with ACLF characterized by Yin jaundice syndrome and protect the liver, with a superior efficacy than the simple comprehensive western therapy.关键词：Yinchen Sini Tang;acute-on-chronic liver failure;Yin jaundice syndrome
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of modified Buzhong Yiqi Tang in treating recurrent spontaneous abortion (spleen kidney deficiency type) caused by negative closed antibody and its effect on levels of serum interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), γ-interferon (IFN-γ), interleukin-10 (IL-10). Method: Seventy-three patients were randomly divided into the experimental group (38 cases) and control group (35 cases) according to the number table method. For control group, patients took spouse in vitro lymphocyte-induce immune therapy. In addition to the therapy of control group, experimental group was given Buzhong Yiqi Tang from the 3rd month before preganecy to the 16 weeks of gestation. For the two groups, they were all followed up until delivery. Conception, the number of weeks exceeding the original gestational weeks, gestational age up to 12 weeks and term birth were recorded. The spleen kidney deficiency syndrome before and after treatment were scored. The conversion of three negative closed antibody (anti-CD3-BE, anti-CD4-BE and anti-CD8-BE) to positive were detected. The levels of serum IL-6, TNF-α, IFN-γ and IL-10 before and after treatment were tested. Result: There was no statistical significance in difference between pregnancy rate and the number of weeks exceeding the original gestational weeks in two groups. The gestational age up to 12 weeks and the rate of term birth in experimental group were superior to these data in control group (P<0.05). After treatment, positive conversion rate of anti-CD3-BE and anti-CD8-BE in experimental group were higher than these data in control group (P<0.05). Levels of TNF-α and IFN-γ in experimental group were superior to these in control group, while the IL-6 and IL-10 were higher than the standards in control group (P<0.01). The total effective rate was 89.47% in experimental group, which was better than 71.43% in control group (P<0.05). Conclusion: Modified Buzhong Yiqi Tang combined with in vitro spouse lymphocyte-induce immune therapy can treat recurrent spontaneous abortion caused by negative closed antibody, promote the positive conversion of closed antibodies, enhance the rate of childbirth pregnancy and relieve clinical symptoms. It has distinct clinical curative effect.关键词：recurrent spontaneous abortion;spleen and kidney deficiency syndrome;negative closed antibody;Buzhong Yiqi Tang;immunotherapy
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of soft capsule of Yindan Xinnaotong combined with DL-thioctic acid in the treatment of diabetic peripheral neuropathy and explore its mechanisms. Method: Ninety-eight patients with diabetic peripheral neuropathy were randomly divided into observation group and control group by the number table method, with 49 patients in each group. The patients in both groups received conventional treatment. The patients in control group were given Lipoic acid injection with 600 mg/time and qd. In addition to the therapy of control group, patients in treatment group were given 4 pieces soft capsules of Yindan Xinnaotong, tid. Both groups received a 4-week treatment. Tactile sensation and temperature sensation were detected by Semmes-weinstein method and Tip Thcrem sensation detector method respectively. Tendon jerk includes knee jerk and ankle jerk. Toronto clinical scoring system (TCSS) was used to evaluate the aforementioned detection. Sensory nerve conduction velocity (SNCV) and motor nerve conduction velocity (MNCV) for median nerve and peroneal nerve in both groups were observed, and blood sugar and blood lipids, the level of serum acetylcholinesterase malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and endothelin-1 (ET-1) were compared between two groups. Result: The total effective rate in the treatment group was 89.8%, which was superior to 73.47% in the control group (P<0.05). After treatment, SNCV and MNCV of median nerve for patients in treatment groups were higher than those of control (P<0.05). After treatment, level of blood sugar was decreased in both groups (P<0.05), but with no significant difference between them. Blood lipids were obviously decreased in treatment group than before the treatment (P<0.01). After treatment, levels of SOD and NO in the treatment group were higher than those of control group (P<0.05), while levels of MDA and ET-1 in the treatment group were lower than the control group (P<0.05). Conclusion: The curative effect of soft capsule of Yindan Xinnaotong combined with DL-thioctic acid is efficient in treating diabetic peripheral neuropathy, and its mechanism may be related with adjustment of the organism lipid metabolism and levels of MDA, SOD, NO and ET-1.关键词：diabetic peripheral neuropathy;soft capsule of Yindan Xinnaotong;DL-thiocitic acid;nerve conduction velocity
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of oral administration of Bushen Quhan Zhiwang decoction in the treatment of kidney deficiency and blood stasis type knee osteoarthritis(KOA) and its effect on levels ofinterleukin-6(IL-6) and tumor necrosis factor(TNF-α) in serum and synovial fluid. Method: One hundred thirty-seven patients with kidney deficiency and blood stasis type KOA were divided into control group(68 cases)and treatment group (69 cases) by random number table.Three and four cases were dropped during treatment from control group and treatment group respectively.For control group, patients took glucosamine hydrochloride tablets (480 mg/time, 3 times/d).In addition to the therapy of control group, patients in treatment group were orally administered with Bushen Quhan Zhiwang decoction(1 dose/d, bid) and lontophoresis(30 min/time, 1 time/d). Course of treatment were 6 weeks for both groups.The Western Ontario and McMaster Universities(WOMAC) score was used to evaluated the symptoms and signs of patients in two groups before and after treatment.The hemorheological indexes and levels of IL-6 and TNF-α in the serum and synovial fluid were detected before and after treatment. Result: The effective rate of control group was 87.69%, which was superior to 96.92% of treatment group(P<0.05). The scores for daily activities, joint stiffnessand pain and total score of WOMAC of treatment group were lower than those of control group(P<0.01).The hemorheological indexes in treatment group were superior to those in control group(P<0.01).The levels of IL-6 and TNF-α in the serum and synovial fluid in treatment group were lower than that in control group(P<0.01). Conclusion: Bushen Quhan Zhiwang decoction for oral administration shows significant efficacyin the treatment of kidney deficiency and blood stasis type KOA, and its mechanism may be related with improving blood circulation and reducing inflammatory reaction.关键词：Bushen Quhan Zhiwang decoction;knee osteoarthritis;hemorheology;interleukin-6;tumor necrosis factor-alpha
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic efficacy of Huopu Xialing decoction for oral administration combined with plaster application on Shenque acupoint in treatment of damp heat-type infantile persistent diarrhea and their effect on the serum level of (IL-10), (IL-23) and (TNF-α). Method: Patients with damp heat-type infantile persistent diarrhea were divided into control group and observation group by random number table, with 60 cases in each group. For control group, patients took Dioctahedralsmectite. Patients in observation group were treated with oral administration of Huopu Xialing decoction combined with plaster application on Shenque acupoint. Course of treatment were 3 d for both groups. Total effective rate, frequency of diarrhea at 1, 2, 3 d after treatment, and stool routine were compared between both groups. The serum levels IL-10, IL-23 and TNF-α were detected before and after treatment. Result: The total effective rate of the treatment and control groups was 93.33% and 83.33% respectively with a significant difference between them (P<0.05). At the second and third day after the treatment, the frequency of diarrhea for observation group was less than that of control group (P<0.01). After treatment, indexes of stool routine in both group was higher than before the treatment, and abnormal cases for WBC and RBC of observation group was less than that of control group (P<0.05). After treatment, serum level of IL-10 in observation group were obviously higher than control group, while IL-23 and TNF-α of observation group were lower than that of control group (P<0.01). Conclusion: Oral administration of Huopu Xialing decoction combined with plaster application on Shenque acupoint in treatment of damp heat-type infantile persistent diarrhea shows significant efficacy, and its mechanism may be related with increasing serum levels of IL-10 and inhibiting IL-23 and TNF-α.关键词：Huopu Xialing decoction;plaster application;infantile persistent diarrhea;interleukin-10;interleukin-23;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Jianpi Yishen Quyu decoction in treating diabetic nephropathy (DN) and its effect on serum levels of inflammatory factors. Method: Eighty patients were randomly divided into control group (40 cases) and research group (40 cases) by random number table. Patients in control group took telmisartan tablets, 40 mg/time, 1 time/day. In addition to the therapy of control group, patients in research group were given Jianpi Yishen Quyu decoction, 1 dose/day. Treatment courses in two groups lasted for 3 months. Before and after treatment, 24-hour urine protein quantitation (24 h UPQ), ratio of microalbuminuria and creatinine (ACR) and cystatin C (CysC) were recorded. And spleen and kidney deficiency and blood stasis blocking collaterals were scored. Levels of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected. Result: Total effective rate in research group was 75%, which was superior to 52.5% of control group (P<0.05). After the treatment, the SCr, BUN, 24 h UPQ, UAER, ACR and CysC in the research group were inferior to these in control group (P<0.01). The scores of spleen and kidney deficiency and blood stasis and collateral blocking symptoms in research group reduced to below these in control group at the first, second and third months after the treatment (P<0.01). The scores of spleen and kidney deficiency and blood stasis and collateral blocking in research group decreased faster than that in control group after treatment (P<0.01). Levels of serum hs-CRP, IL-6, TNF-α in research group were lower than these standards in control group (P<0.01). Conclusion: In addition to the conventional western medicine therapy, Jianpi Yishen Quyu decotion can be administered to improve DN renal function at early stage, reduce albuminuria and glomerular filtration rate, relieve the clinical symptoms and inflammation reaction. Its clinical curative effect is superior to that of simple western medicine treatment measures.关键词：diabetic nephropathy;Jianpi Yishen Quyu decotion;renal function
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic efficacy of Maxing Wuzi decoction on acute exacerbation of pulmonary heart disease (phlegm-damp obstructing lung type) and its effect on plasma levels of endothelin (ET)-1 and D-dimer. Method: One hundred and ten patients with acute stage of pulmonary heart disease (phlegm-damp obstructing lung type) were randomly divided into control group and treatment group by random number table, with 55 patients in each group. Patients of control group took routine western medicine therapy. In addition to the therapy of control group, patients in treatment group were give Maxing Wuzi decoction, twice/day, 1 dose/day. Course of treatment lasted for 4 weeks in both groups. After treatment, the curative effects in the two groups were evaluated, and changes of blood gas, hemorrheology, cardiac output and mean pulmonary arterial pressure were monitored and compared between both groups. Before and after treatment, plasma ET-1 and D-dimer levels were measured. Result: The total effective rates of control group and treatment group were 56.36% and 87.27% respectively, and treatment group was significantly higher than that of control group (P<0.01). After treatment, PaCO2 for treatment group was lower than that of control group, and PaO2 was higher than control group (P<0.01). Various hemorrheological indexes of observation group were obviously lower than that of control group (P<0.05, P<0.01). After treatment, cardiac output of treatment group was higher than control group, and mean pulmonary arterial pressure was lower than control group (P<0.01). Plasma levels of ET-1 and D-dimer in treatment group were lower than those of control group (P<0.01). Conclusion: Curative effect of Maxing Wuzi decoction are efficient in treating acute exacerbations of pulmonary heart disease (phlegm-damp obstructing lung type), and its mechanism may be related with improving blood viscosity and inhibiting levels ET-1 and D-dimer.关键词：Maxing Wuzi decoction;acute exacerbations of pulmonary heart disease;blood gas analysis;hemorheology;endothelin-1;D-dimer
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Jinshiyin prescription combined with extracorporeal shock wave lithotripsy (ESWL) in treating urolithiasis and its effect on serum lipids in patients with urolithiasis. Method: Totally one hundred and twenty cases of eligible urolithiasis patients were divided into two groups according to therapeutic scheme, the control group (n=60) and the observation group(n=60). The control group was treated with ESWL, and the observation group was treated with Jinshiyin prescription combined with ESWL. The follow-up visit lasted for one year. The curative efficacy, recurrence rate, urine culture result and changes in serum lipid between both groups were observed and compared. Result: The observation group showed a therapeutic efficiency ratio of 96.7%, which was significantly higher than that of 83.3% in the control group (P<0.05). After the one-year follow-up visit, the recurrence rate in the observation group was 1.7%, which was statistically lower than that of 13.0% in the control group (P<0.05). Compared with the control group, the observation group showed reductions in the proportion of positive urine culture results, total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL-C) and increase in high-density lipoprotein (HDL-C) after the treatment, with statistical significance in differences (all P<0.05). Conclusion: Jinshiyin prescription combined with ESWL in treating urolithiasis is an effective therapeutic scheme, which can increase the therapeutic efficacy, decrease the recurrence rate and urinary infection and effectively regulate serum lipid.关键词：Jinshiyin prescription;extracorporeal shock wave lithotripsy;urolithiasis;serum lipid
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发布时间：2024-01-04 - 摘要：Objective: To systematically evaluate the efficacy and safety of the adjuvant therapy with traditional Chinese medicines (TCM) in treating perimenopausal insomnia. Method: The PubMed, Cochrane Library, OVID, EMbase, CNKI, WanFang Data and VIP databases between 2010 and December 2014 were searched through computer to collect all RCTs on the comparison of the combined therapy of TCM and western medicines and the simple therapy of western medicines in treating perimenopausal insomnia. The data extraction form was designed. Two researchers independently screened the littérateurs, extracted data and evaluated the literature bias risk according to the inclusion standards. The Meta-analysis was operated by using RevMan 5.2.6 software. Result: A total of 21 RCTs involving 1 608 patients were included. The result of Meta-analysis indicated t hat the TCM adjuvant therapy group was superior to the control group in terms of clinical effectiveness[OR=3.26, 95%CI(2.50, 4.26), P<0.000 01], recurrence rate[OR=4.21, 95%CI(2.46, 7.22), P<0.000 01]and total scale of PSQI[MD=-2.28, 95%CI(-2.78, -1.78)]. With respect to the sleep improvement, the TCM adjuvant therapy group was also better than the control group in quality of sleep[MD=-0.40, 95%CI(-0.73, -0.06)], time of sleeping[MD=-0.54, 95%CI(-0.91, -0.16)], hour of sleep[MD=-0.41, 95%CI(-0.60, -0.22)], efficiency of sleep[MD=-0.38, 95%CI(-0.56, -0.20)], disorders of sleep[MD=-0.37, 95%CI(-0.55, -0.19)], application of hypnotic drugs[MD=-0.32, 95%CI(-0.47, -0.18)], daytime dysfunction[MD=-0.29, 95%CI(-0.47, -0.10)]and scale of Kupperman[MD=-5.48, 95%CI(-9.87, -1.09), P=0.01]. With respect to the syndrome evaluation, the TCM adjuvant therapy group showed a better effect than the conventional treatment group in relieving patients' hot flash and sweating[MD=-1.65, 95%CI(-1.84, -1.45)], irritability[MD=-0.99, 95%CI(-1.13, -0.85)], dizziness and palpitation[MD=-2.18, 95%CI(-2.92, -1.43)]and mental burnout[MD=-1.89, 95%CI(-2.07, -1.72)]. At the level of endocrine hormone, the TCM adjuvant therapy group can better improve the estradiol (E2) level[MD=17.70, 95%CI(1.89, 33.52)]and reduce the level of follicle-stimulating hormone (FSH)[MD=-11.51, 95%CI(-20.49, -2.53)], but with no statistical significance in difference in luteinizing hormone (LH)[MD=-5.85, 95%CI(-13.01, 1.30)]. No serious or frequent adverse effect was reported for the two groups. Conclusion: The Meta-analysis suggests that the adjuvant intervention with TCM can improve patients' sleep disorders, enhance the quality of sleep, with less adverse effect. However, there are only a few research methodologies and reports in a low quality. The above results shall be further proved by well-designed large-sample-size studies.关键词：perimenopausal insomnia;systematic evaluation;Meta analysis;randomizd controlled trial
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发布时间：2024-01-04 - 摘要：Human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) refers to the damage to the body's immune function caused by HIV and symptomized by common fatal opportunistic infections, which can cause organ failure in human bodies and even death. The prevention and treatment of common opportunistic infection of AIDS have important clinical significance to improve the quality of life in patients with HIV/AIDS and prolong their survival time. Physicians have obtained the remarkable clinical curative effect in preventing and treating of AIDS opportunistic infections with traditional Chinese medicine(TCM). Based on the summary of early-stage clinical practice in treating AIDS diarrhea, pulmonary infection, fever, herpes zoster and other common opportunistic infections with TCMs, the researchers put forward the accurate positioning of opportunistic infection of AIDS treatable by TCMs, conducted early interventions according to its etiology, pathogenesis and characteristics, studied effective prescriptions and treatment plans, took advantage of the synergy of combining traditional Chinese and western medicine, expanded the application scope and research thoughts for TCMs in preventing and treating AIDS, so as to better enhance their clinical efficaciy.关键词：human immunodeficiency virus/acquired immunodeficiency syndrome;opportunistic infections;practices;thoughts
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发布时间：2024-01-04 - 摘要：Drugs can play its efficacy only when their concentration accumulates to a certain degree at target sites. The accumulated concentration is not only related to the drug dosage, but also closely correlated with the processes of absorption, distribution, metabolism, and excretion in organisms. Since the pharmacokinetic behaviors of drugs may significantly change in pathological conditions, it is of great significance to study drug pharmacokinetics in disease conditions and rationally design clinical dosage regimens. Therefore, to provide certain reference of rational clinical drug administration of traditional Chinese medicines(TCMs) and their preparations, efforts shall be made to analyze the impacts of some clinical common diseases on the absorption, distribution, metabolism, and excretion of TCMs and the impacts of different lesions and their interactions on the metabolic process of TCMs.关键词：disease;absorption;distribution;metabolism;excretion
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发布时间：2024-01-04 - 摘要：Lotus leaves (Nelumbo nucifera) are fresh or dried leaves of perennial aquatic plant N. nucifera, which are commonly used as a traditional Chinese herb as well as food. The optimal harvest seasons of lotus leaves are summer and autumn. Lotus leaves are mostly distributed and cultivated in drainage basins of Yellow River, Changjiang River and Zhujiang River in China. Modern research has shown that flavonoids and alkaloids are the principal constituents in lotus leaves. Along with the development modern research technologies, in-depth studies have showed that quercetin, isoquercetin and kaempferol are the main flavonoid aglycines, and isoquinoline alkaloids, aporphine alkaloids and its derivatives are the principal alkaloids. Lotus leaves have extensive pharmacological activities such as antioxidant, anti-obesity, anti-human immunodeficiency virus(HIV), anti-cancer and anti-cardiovascular disease activity, with a better medicinal value. Based on the domestic and foreign literatures, theses and dissertations in the past 10 years, relevant researches for flavonoid and alkaloid constituents of lotus leaves were summarized in order to provide references for further studies and production, which included the chemical composition, extraction and isolation progress, quality assessment methods and the pharmacological activity. Based on the current studies, for the purpose of rational development and utilization of its plant resources, further studies shall be made for the extraction and purification technology, the quality criteria technology and the target localization technology.关键词：Nelumbo nucifera;chemical constituent;extraction and isolation progress;quality assessment methods;pharmacological activity
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发布时间：2024-01-04 - 摘要：Islet beta cell dysfunction and decrease play an important role in the occurrence and development of type 2 diabetes mellitus(T2DM). The effect of traditional Chinese medicine (TCM) on the protection of islet beta cells was recognized and plays a crucial role in the prevention and control of type 2 diabetes. The protective mechanism of TCMs' extracts, monomer and compounds to islet beta cells were summarized to define their effect in protecting islet beta cells and treating T2DM. Some TCMs can promote islet beta cell proliferation, reduce islet beta cells apoptosis, and protect islet beta cells indirectly by promoting islet beta cell proliferation, reducing islet beta cell apoptosis, improving serum and pancreatic tissue oxidative stress, improving islet cell insulin resistance, improving the level of glucagon like peptide-1(GLP-1), improving pancreas microcirculation, regulating autophagy level. Because of the complexity of the traditional Chinese medicine ingredients and compound formula, their protective effect on islet beta cells involves a variety of targets and mechanisms. Besides, under the guidance of general concept of TCMs, TCMs have a higher safety in treating T2DM. Therefore, TCMs have a broad prospect to protect islet beta cells. With the development of biotechnology, it is possible to screen out new diabetes drugs from TCMs.关键词：islet beta cells;proliferation;apoptosis;oxidative stress;islet cell insulin resistance;glucapon like peptide-1;pancreatic microcirculation;autophagy
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发布时间：2024-01-04 - 摘要：The advance in studies on the quality control of Scrophulariae Radix was summarized. Literatures in recent years were studied to define the main active ingredient in S. ningpoensis is iridoid glycosides. Although Scrophulariae Radix has grown in most areas of China, there is no improved quality standards and norms and sufficient quantitative control, which resulted in differences in quality control. The current development trend for controlling traditional Chinese medicine quality is to diversify the quality control mode and establish the multi-mode multi-chromatography system and its coupling technique quality control method. Currently, the quality control mode for Scrophulariae Radix is dominated by single index, multi-index quality control and fingerprint;HPLC, high performance capillary electrophoresis (HPCE), near-infrared spectroscopy (NIRS), attenuated total reflectance (ATR) and other techniques lay a foundation for exploring methods and new techniques for systematic control and evaluation for Scrophulariae Radix. The above content provides the reference for chemical components and quality control of Scrophulariae Radix and the basis for further development and utilization of Scrophulariae Radix resources.关键词：Scrophulariae Radix;quality control;microscopical identification;HPLC;new technology
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发布时间：2024-01-04 - 摘要：Dipsacaceae Scabiosa plants are widely applied in traditional folk herbal medicines at home and abroad, with a great potential medical value. The recent literatures on chemical constituents in Scabiosa and pharmacological effects were collected from SciFinder, PubMed, CNKI, Wanfang date and multiple domestic and foreign batabases. The literatures were analyzed and summarized in to reviews. So far, various chemical constituents, such as flavonoids, triterpennoids, iridoids, coumarins, organic acids, phenolic constituents and volatile oils, were isolated from Scabiosa plants. Their pharmacological effects included antipyresis and anti-inflammation, anti-oxidation, anti-platelet aggregation, sedation and immuno-enhancement, particularly in anti-oxidation and anti-bacteria. Flavonoids and terpenoids were its main effective components. Based on the domestic and foreign studies on Scabiosa plants, the advance in studies on structures and pharmacological effects of the chemical constituents were summarized to provide a reference for the comprehensive study and utilization of Scabiosa plants, the discovery of new active pharmaceutical ingredients and the protection of its resources.关键词：Scabiosa;chemical constituent;pharmacological activity
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发布时间：2024-01-04 - 摘要：Objective: Acronychia pedunculata, as a type of characteristic authentic medicinal herbs growing in Hainan, Guangdong, Guangxi, Yunnan and other provinces in China, was recorded in China Collection of Chinese Herbal Medicines, Illustrations of Chinese Medicinal Plantsand Colored Atlas of Chinese Folk Herbs. It mainly contains alkaloids, phenolic and essential oil constituents, with a fragrant smell, sweet taste and slight tongue numbness. It can be used as a food and medicine, with effects in treating cold and cough, helping digestion, stopping bleeding, killing pain, detumescence, promoting tissue regeneration, relieving asthma, anti-fever and stopping dysentery. According to modern pharmacological studies, A. pedunculata has anti-tumor, antibiotic and cytotoxic. This paper mainly focuses on the overview of studies on chemical constituents and pharmacological action of A. pedunculata, in order to provide reference for further comprehensive utilization and development of its resources.关键词：Acronychia pedunculata;chemical constituent;pharmacological activity
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发布时间：2024-01-04
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