最新刊期
卷 21 ， 期 19 ， 2015
- 摘要：Objective: To optimize purification process of total flavonoids from four Crataegus fruits in Xinjiang by macroporous resin and evaluate its antioxidant activity. Method: With purity of total flavonoids as index, 13 macroporous resins for adsorption and separation of total flavonoids were compared.Purification process of total flavonoids was optimized by screening flow velocity of sample, concentration of eluent.Antioxidant activity was determined by 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) and 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method.Contents of rutin and hyperin were determined by HPLC with mobile phase of acetonitrile-0.4% formic acid (16:84) and detection wavelength at 360 nm. Result: HPD722 resin offered the best adsorption and desorption capacity for total flavonoids.Optimal purification process was as follows:flow velocity of sample 0.84 mL·min-1, after reaching adsorption balance, dynamically eluted with 40% ethanol.After separated and purified by HPD722 resin, contents of total flavonoids, rutin, hyperin and antioxidant capacity increased 1.9-3.3, 2.4-5.1, 2.6-3.7, 2.0-4.6 times, respectively. Conclusion: Total flavonoids from four C. fruits can be effectively purified and enriched by HPD-722 macroporous resin.关键词：Crataegus pinnatifida var. major;C. pinnatifida;C. chlorocarpa;C. sanguine;total flavonoids;purification;antioxidant activity
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technique of Cuochuangxiao gel and provide a reference for clinical application of this preparation. Method: With composite score of contents of free anthraquinones in Rhei Radix et Rhizoma, gardenin and caffeic acid as index, uniform design was adopted to investigate effects of ethanol concentration, ethanol volume, extraction time and times on extraction process of Cuochuangxiao gel.HPLC was employed to determine contents of free anthraquinones in Rhei Radix et Rhizoma, gardenin and caffeic acid with mobile phases of methanol-0.1% phosphoric acid (85:15), methanol-water (23:77) and acetonitrile-0.1% phosphoric acid (10:90), detection wavelengthes of 254, 238, 323 nm, respectively. Result: Optimum extraction conditions were as follows:added 12 times the amount of 80% ethanol, extracted 3 times for 1 h each time.Under these conditions, contents of free anthraquinones in Rhei Radix et Rhizoma, gardenin and caffeic acid were 1 260.33, 1 597.56, 32.19 mg·L-1, respectively. Conclusion: This extraction process is reasonable, stable and feasible, which provides a theoretic basis for industrial production of Cuochuangxiao gel.关键词：Cuochuangxiao gel;aloe-emodin;chrysophanol;physcion;gardenin;caffeic acid
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To choose the best DNA extraction method for different kind of Tupistra chinensis materials. Method: Sodium dodecyl sulfate (SDS), improved SDS, hexadecyl trimethyl ammonium bromide (CTAB) and improved CTAB methods were used to extract DNA from five different materials of T. chinensis tubers and leaves, including fresh tubers, silica gel dried tubers, baked tubers, fresh leaves and silica gel dried leaves.Integrity, purity and yield of extracted DNA was compared in order to determine which method was the best one for different T. chinensis materials. Result: Extracting DNA from silica gel dried tubers and silica gel dried leaves with improved CTAB method performed the highest purity and good integrity, not only its value of A260 nm/A280 nm was between 1.8-1.9, but also with a better integrity.These remaining materials had different levels of pollution, A260 nm/A280 nm of fresh leaves was generally low. Conclusion: Improved CTAB is the most effective method for DNA extraction from silica gel dried leaves and tubers materials.Genomic DNA extracted from silica gel dried leaves and tubers using improved CTAB method performs a better integrity and better purity.关键词：Tupistra chinensis;DNA extraction;purity;fresh tubers;integrity;improved CTAB method
- 5
- |
- 3
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To prepare betaxolol hydrochloride-montmorillonite ion exchange microspheres eye suspension. Method: Excipients of betaxolol hydrochloride-montmorillonite ion exchange microspheres eye suspension were screened and its preparation characters were evaluated, biological properties of microspheres suspension were investigated by eye irritation and isolated corneal permeability test. Result: Optimal combination was 0.15% carbomer 934P as suspending agent and 0.01% tween-80 as wetting agent.Its stability, pH value, osmotic pressure, viscosity and corneal irritation were complies with the requirement of ophthalmic preparations applications.During irritation test, there was no obviously irritating in the microspheres suspension group, but the Beiteshu group's conjunctiva appeared partly lymphocytic infiltrates.Isolated corneal permeability test showed that the suspension's apparent permeability coefficient (Papp) was 1.78×10-5 cm·s-1, half of betaxolol hydrochloric solution (3.57×10-5 cm·s-1) and twice of Beiteshu drops (0.85×10-5 cm·s-1). Conclusion: It is shows that self-made ion exchange microspheres suspension possesses capability of sustained-release and extenuate drug toxicity.关键词：montmorillonite;betaxolol hydrochloride;ion exchange microspheres;irritation;corneal permeability
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To prepare curcumin-quinacrine liposomes, then optimize its formulation technology and evaluate its quality in order to provide references for development of curcumin. Method: Curcumin-quinacrine liposomes were prepared by film dispersion-ammonium sulfate gradient method.The content of curcumin and quinacrine were determined by HPLC, mobile phase was methanol-water (containing 0.3% acetic acid) (70:30) and acetonitrile-water (containing 0.6% acetic acid and 1.5% triethylamine) (50:50), flow velocity was 1.0, 1.5 mL·min-1, detection wavelength was 423, 269 nm, respectively.Formulation process of curcumin-quinacrine liposomes was optimized by single factor tests with encapsulation efficiency as index.Characterization of curcumin-quinacrine liposomes were investigated, including particle size and Zeta potential. Result: Parameters of optimal prescription were mole ratio of phospholipid-cholesterol (2:1), curcumin-membrane material (1:40), quinacrine-membrane material (1:48) and ammonium sulfate concentration of 250 mmol·L-1.Encapsulation efficiencies were (95.33±0.85)% and (94.13±0.49)% for curcumin and quinacrine, respectively.Particle size was (117.73±0.15) nm, Zeta potential was (-8.56±0.22) mV, polydispersity index (PDI) was 0.12±0.01. Conclusion: Curcumin-quinacrine liposomes has advantages of uniform particle size, high encapsulation efficiency, good stability and enough slow-release effect.关键词：curcumin;quinacrine;liposomes;encapsulation efficiency
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To discuss preparation process parameters of Salviae Miltiorrhizae Radix et Rhizoma extract in mass production. Method: Single factor tests were adopted, pretreatment of raw materials of tanshinone extract was investigated by taking contents of cryptotanshinone and tanshinone ⅡA as indicators.Water extraction temperature of Salviae Miltiorrhizae Radix et Rhizoma was optimized by taking solid-containing content and the content of salvianolic acid B as indexes.With solid-containing content, the content of salvianolic acid B and fingerprints as indicators, raw materials of water extraction for Salviae Miltiorrhizae Radix et Rhizoma were determined.Fingerprints was employed to determine optimum concentration temperature of water extract of Salviae Miltiorrhizae Radix et Rhizoma. Result: Pretreatment of raw materials of tanshinone extract was cutting into small pieces of 1-2 cm.Raw materials of total phenolic acid extract in Salviae Miltiorrhizae Radix et Rhizoma can use the residues of tanshinone extract, extraction temperature was revised to 100℃, the concentrated temperature was revised to 80℃. Conclusion: These optimized process conditions are stable and feasible, which can provide experimental basis for large-scale production of Salviae Miltiorrhizae Radix et Rhizoma extract.关键词：Salviae Miltiorrhizae Radix et Rhizoma;tanshinone extract;salvianolic acid B;Cryptotanshinone;fingerprint
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate antioxidant activities and inhibitory effect on protein tyrosine phosphatase1B (PTP1B) of different fractions of Rubus idaeus, R.caesius and Ribes meyeri in Xinjiang, in order to screen out active ingredients with antioxidant activity and hypoglycemic action. Method: The different polar fractions were extracted by methanol and fractional extraction method. Then the total flavonoids content of different polar fractions were determined. The anthocyanin fractions of fruits were purified by AB-8macroporous resin. ABTS method, DPPH method and PTP1B inhibitory activity experiment were adopted to compare relevant activities of different fractions and analyze the correlation of flavonoid content and activity. Result: After AB-8macroporous resin purification, anthocyanin contents of R.idaeus and R.meyeri fruit extract increased from (12.7±0.14) mg·g-1 to (238.1±1.18) mg·g-1, and (35.3±0.45) mg·g-1 to (548.0±1.11) mg·g-1, respectively. With the increase of anthocyanin content, antioxidant activity and PTP1B inhibitory activity were also significantly increased. The ethyl acetate fraction of R.caesius stem had the highest total flavonoids content, which was (658.65±11.43) mg·g-1. The water fraction of R.caesiusstem had the highest antioxidant activity and PTP1B inhibitory activity, which was IC50(0.08±0.01) mg·L-1, and their ABTS and DPPH radical scavenging capacity were (4.53±0.07) and (2.82±0.09) mmol Trolox·g-1 equivalent, respectively. Conclusion: The different fractions of three plants have high flavonoid contents and good antioxidant activity and PTP1B inhibitory effect. The water fraction of R.caesius stem was the main active fraction. Anthocyanin of R.idaeus and R.meyeri fruits was the main active ingredient, and flavonoids from stem and leave of R.meyeri was the main antioxidant ingredient.关键词：Rubus idaeus;Rubus caesius;Ribes meyeri;protein tyrosine phosphatase 1B;antioxidantion
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To estabilish an HPLC fingerprint of Fritillariae Thunbergii Bulbus to provide a comprehensive evaluation for its quality control. Method: Welch XtimateTM C18 (4.6 mm×250 mm, 5 μm) column was used and eluted with a mobile phase of acetonitrile-10 mmol·L-1ammonium acetate solution (pH 10.0) in a gradient mode, the flow rate was 0.8 mL·min-1, the column temperature was 25℃. An evaporated light scattering detector was used to determine the fingerprint. The fingerprint was further evaluated by chemometric methods, including similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA). Result: The precision, stability and repeatability of the method were favorable. Seven chromatographic peaks were extracted as the common peaks of fingerprint, and 16 batches of samples were compared and classified with such methods as SA, HCA and PCA, and then divided into 2 clusters by their quality difference. Conclusion: The method is reproducible, simple and reliable and can be used for the quality control and evaluation of Fritillariae Thunbergii Bulbus.关键词：Fritillariae Thunbergii Bulbus;HPLC-ELSD;fingerprint;chemometrics;quality evaluation
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To synthesize the novel derivatives with the natural resource 18α-glycyrrhetinic acid and evaluate their antitumor activity. Method: The novel 18α-glycyrrhetinic acid derivatives (7 and 8) were synthesized by combining the matrine and nitrogen mustard melphalan with the 18α-glycyrrhetinic acid. The target compounds were characterized by 1H-NMR, MS and elemental analyses. Their antitumor activities against SMMC-7721 cell lines and their toxicities on normal BRL cell lines were evaluated by MTT assay in vitro. Result: The compound 7 exhibited a higher antitumor activity and nontoxicity in vitro. In the in vivo test in mice, with the dose of 6, 9 μmol·kg-1, the inhibition rates of compound 7 in HepA tumor growth were 40.72% and 60.12%, which were higher than parent drug. Meanwhile, with the dose of 6 μmol·kg-1, the inhibition rate of melphalan was 39.93%. Conclusion: The compound 7 exhibited a high antitumor activity against hepatoma cells, which is worth further studying and developing.关键词：18α-glycyrrhetinic acid;derivatives;combination;traditional Chinese medicine;antitumor
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To analyze X-ray diffraction (XRD) and Raman spectrum fingerprint characteristics of Fluoritum, and to provide a reference for its fast and effective identification. Method: XRD was used to determine phase composition and the content of CaF2 from Fluoritum, Calcite and Quartz with Cu target Kα radiation as incident light source, filter of Ni, 40 kV as X-ray tube voltage, 40 mA as the current, 1 degree as divergence slit (SD) and scattering slit (SS), 0.3 mm as receiving slit.On this basis, Raman spectrum characteristics of Fluoritum in the range of 61-2 695 cm-1, with 785 nm as wavelength of excitation light source, 300 mV as laser power, 1.5 m optical fiber probe and laser intensity of 100%. Result: Analysis of XRD indicated that samples 1-15 were Fluoritum, samples 16-20 were adulterants and sample 21 was counterfeiter.Fluoritum had 3 groups of characteristic peaks of Raman spectrum in the range of 310-325, 720-1 500, 1 700-1 900 cm-1, which can be used to identify its authenticity. Conclusion: XRD can accurately determine phase composition of Fluoritum, which provides original sample data to identify Fluoritum by Raman spectrum.As a way of fast analysis technology, Raman spectroscopy has good specialization, it can identify Fluoritum and its adulterants, counterfeiters.关键词：fluoritum;X-ray diffraction;phase;Raman spectrum;fingerprint characteristics;cluster analysis
- 6
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To investigate the diterpenoid alkaloids from the roots of Aconitum Penduli Radix and their bioactivities. Method: The compounds were isolated by positive-phase silica gel column chromatography, Sephadex LH gel column chromatography and semi-preparative HPLC. The structures of the isolated compounds were determined with chemical and spectroscopic methods. The cytotoxic activities were assessed by MTT. Result: From the roots of Aconitum Penduli Radix, 8 diterpenoid alkaloids were isolated and identified as 3-deoxyaconitine(1), 3-acetylaconitine(2), aconitine(3), hypaconitine(4), 8-O-methyl-14-benzoylaconine(5), spicatine A(6), aldohypaconite(7), hokbusine A(8). Compound 3 showed the potent cytotoxicity against A549 and 1299 lung cancer cells and compounds 2, 4-8 showed the lower cytotoxicity. Conclusion: Diterpenoid alkaloids are the main and effective chemical components from from Aconitum Penduli Radix. Specifically, compounds 6-8 were obtained from the roots of Aconitum Penduli Radix for the first time. Compounds 2-8 showed a certain cytotoxicity.关键词：Aconitum Penduli Radix;diterpenoidalkaloids;chemical constituent;cytotoxicity
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents of volatile oil from Zelkova schneideriana leaves with GC-MS technology. Method: The volatile oil was extracted from the leaves by steamdistillation. The constituents of volatile oil were identified by GC-MS technology. Result: Relative content of volatile oil was determined by peak area normalization. Totally 64 compounds were identified, accounting for 71.06% of the total volatile oil content. The principal chemical constituents of the essential oils were ethyl propionate (3.61%), butyl acetate (8.28%), trans-2-hexenal (1.66%), N-propylbenzene (1.46%), 4-ethyltoluene (6.18%), trimethylbenzene (5.61%), linalool (1.09%), 6-methyl-3, 5-hep-tadien-2-one (1.43%), geranyl acetone (1.43%), beta-ionone (1.12%), palmitaldehyde (1.25%), myristicacid (1.07%), farnesyl acetone (1.89%), dibutyl phthalate (10.82%), phytol (5.72%). Conclusion: The results provide reliable experimental data and theoretical basis for the further development and utilization of Zelkova schneideriana leaves.关键词：Zelkova schneideriana leaves;volatile oil;GC-MS
- 5
- |
- 5
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC-MS/MS method for simultaneously determining five components (chronogenic acid, phillyrin, forsythoside A, luteoloside, rutin) in Shuanghuanglian tablets. Method: Waters Acquity BEH C18column (2.1 mm×100 mm, 1.7 μm) was adopted and eluted with the mobile phase of acetonitrile and 0.1% formic acid/water in a gradient mode at a flow rate of 0.4 mL·min-1. The analytes were detected by tandem mass spectrometry with the electrospray ionization (ESI) source in the multiple reaction monitoring (MRM) mode. Result: Ten compounds showed a good linear relationship (r>0.999);the precisions, repeatabilities and stabilities of the method were good, and the average recoveries were between 96% and 104.67% with the relative standard deviations of no more than 3%. Conclusion: The established method is highly repeatable, rapid and accurate and can be used for the determination of five effective components in Shuanghuanglian tablets.关键词：UPLC-MS/MS;Shuanghuanglian tablets;chronogenic acid;phillyrin;forsythoside A;luteoloside;rutin
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC fingerprint method of water extracts from Portulaca oleracea. Method: The sample solutions of P. oleracea from different origins were prepared by the reflux extraction. An HPLC fingerprint analysis was performed with acetonitrile-methanol (7:3) and 0.1% formic acid as the mobile phase by gradient elution. The UV detection was 320 nm. And the similarity evaluation analysis was conducted. Result: Totally 11 common peaks were identified in the fingerprints of sample solution of 10 types of P. oleracea from different origins. With the No.5 peak caffeic acid as the reference peak, the similarities of 12 common peaks in the fingerprints of the 10 batches of samples were more than 0.80. Conclusion: The method has a good reproducibility and stability and identify 11 common peaks in the HPLC fingerprint, with a high overlap rate in the chromatographic peaks, indicating no significant regional differences in P. oleracea. It can provide a reliable basis for the quality control of P. oleracea and a reference for relevant and clinic application studies.关键词：Portulaca oleracea;fingerprint;HPLC;different places
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for the determination of quercetin and kaempferol in Dicranopiers dichotoma, and to determine the content of quercetin and kaempferol in D. dichotoma from different areas. Method: Different volume fractions of methanol and ethanol were chosen as the extraction solvents for quercetin and kaempferol. They were extracted by ultrasonic extraction and refluxing process and determined by HPLC. Diamonsil C18 column (4.6 mm×200 mm, 5 μm) was adoped, with the mixture of methanol-0.1%H3PO4 water (54:46) as the mobile phase. The flow rate was 1.0 mL·min-1, and the column temperature at 30℃, the detection wavelength was set at 366 nm. Result: Refluxing process with 80% methanol was proved to be the optimal extraction method for quercetin and kaempferol, and the content of quercetin and kaempferol in D. dichotoma from Yingtan of Jiangxi Province was more than that from other areas. This method had a good separation performance for quercetin and kaempferol in D. dichotoma, and the linear range were 1.051-16.24, 0.505-8.08 μg, respectively. Conclusion: This method is accurate, sensitive, stable and reliable, and can be used in the quality control of D. dichotoma. The contents of quercetin and kaempferol in D. dichotoma of different origins were significantly different.关键词：Dicranopiers dichotoma;quercetin;kaempferol;HPLC
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To establish a method to determine the contents of heavy metal elements in Tanshinone ⅡA. Method: ICP-MS, AAS and AFS were used to determine the contents of metal elements with Sc, Y, In and Tb as the internal labels after microwave digestion of samples. Result: Each metal elements had a good linear relationship (correlation coefficient >0.999 7), detection limit within 0.03 μg·kg-1 to 2.16 μg·kg-1and the average recovery value ranged from 95% to 113%. Conclusion: The method was simple in operation, and excellent in precision, accuracy, stability, and repeatability, which can be recommended to determine the content of metal elements in Tanshinone ⅡA. It also can provide reference for accurate determination and quality control of metal elements in other traditional Chinese medicine extracts.关键词：microwave digestion method;ICP-MS;AAS;AFS;Tanshinone ⅡA;determination of metal elements
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of scutellarin, 3, 5-dicaffeoylquinic acid (3, 5-DCQA), 3, 4-dicaffeoylquinic acid (3, 4-DCQA) in extract of Erigerontis Herba. Method: The HPLC method was carried out on Dalian Elite C18 column (4.6 mm×250 mm, 5 μm) evaluated with acetonitrile-0.1% phosphoric acid (22:78) as mobile phase, the flow rate was 1 mL·min-1, the temperatue of column was at 35℃, the detection wavelength was at 330 nm for UV detection. Result: The calibration curves were linear in the range of 0.032 7 to 1.308 0 μg (r=0.999 9) for scutellarin, 0.048 2 to 1.928 0 μg (r=0.999 9) for 3, 5-DCQA, 0.058 3 to 2.332 0 μg(r=0.999 8) for 3, 4-DCQA, respectively. The average recoveries were 99.74% with RSD 1.9%; 100.23% with RSD 1.4% and 99.33% with RSD 1.3% respectively. Conclusion: The assay demonstrated that the method was simple, it had adequate accuracy and selectivity to quantify the three active components in extract of Erigerontis Herba.关键词：extract of Erigerontis Herba;scutellarin;3,5-dicaffeoylquinic acid;3,4-dicaffeoylquinic acid;determination
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study excretion characteristics of senkyunolide I in urine and bile by intravenous injection and intragastric administration, respectively. Method: Rats were intravenous injected and intragastric administrated with senkyunolide I at a dose of 72 mg·kg-1, respectively.The urine and bile were collected at different time points.The concentrations of senkyunolide I in urine and bile were measured by HPLC-VWD, detection wavelength was set at 278 nm, mobile phase consisted of acetonitril-water (containing 0.1% formic acid) for gradient elution. Result: After intragastric administration and intravenous injection, cumulative excretion of senkyunolide I in 48 h were (179.8±33.68), (264.8±87.28) μg in urine, cumulative excretion percentages were calculated to be (0.77±0.15)% and (1.35±0.26)%, respectively;cumulative excretion of senkyunolide I in 36 h were (359.4±75.66), (426.3±140.90) μg in bile, cumulative excretion percentages were calculated to be (1.22±0.49)% and (1.72±0.59)%, respectively. Conclusion: Prototype is not main elimination form of senkyunolide I in rats' urine and bile, its speed of excretion is fast.关键词：senkyunolide I;cumulative excretion;urine;bile;Chuanxiong Rhizoma
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To study the effects of Qingfei oral liquid on airway inflammation and hypersensitivity in a respiratory syncytial virus (RSV) infected murine asthma model. Method: Sixty 6-8-week-old male Balb/C mice were equally divided into six groups by random:the normal group (CON), model group (RSV+OVA), Qingfei oral liquid group [high dose (HD), middle dose (MD), low dose (LD)], and ribavirin group (LB).The asthma model was reproduced by sensitization and nebulizer inspired OVA, followed by repeated inhalation of RSV for three consecutive times to obtain RSV-infected asthma model (1.0×106 PFU/mL in 50 μL). Qingfei oral liquid groups were administered with different doses. The normal control group was administered with normal saline. LB group received ribavirin injection ip 24 h after of the last administering, Buxco RC system was used to detect airway hyperresponsiveness, ESO cells infiltration were measured in bronchoalveolar lavage fluid (BALF) fluid, and inflammation was assessed in lung tissue by HE staining, PAS staining, VG staining. Result: The proportion of BALF's eosinophils (EOS%) in model group was significantly higher than that in the normal group (P<0.05).Airway hyperresponsiveness in model group was significantly higher than that in normal group (P<0.01). Qingfei oral liquid and Ribavirin cleared RSV's infection and significantly improved airway hyperresponsiveness compared with the model group (P<0.05), among which Qingfei oral liquid middle-dose group had the most significant effect. There was no significant difference in airway functions compared with normal group. Qingfei oral liquid middle-dose group significantly reduced lung tissue edema and inflammatory cell infiltration, airway secretions and collagen hyperplasia around airway compared with model group. Conclusion: Qingfei oral liquid middle-dose group can significantly reduce inflammation of lung tissues and airway hyperresponsiveness in RSV infected murine asthma model.关键词：Qingfei oral liquid;respiratory syncytial virus;asthma;airway inflammation;airway hypersensitivity
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To analyze the effects of traditional Chinese medicine Shuyu Ningxin recipe on on the praxiology and the expressions of hippocampal brain-derived neurotrophic factor (BDNF) and its tyrosine kinase B (TrkB) expression in rats with chronic stress-induced depression. Method: Sixty male SD rats were randomly divided into normal control group, model group, western medicine group (fluoxetine), as well as Shuyu Ningxin recipe low dose group, middle dose group and high-dose group (2.5, 7.5, 25.0 g·kg-1respectively), with 10 models in each group. All other groups except normal control group were subjected to establish chronic stress-induced depression model for a period of 21 days. Starting from two weeks after modeling, the medicine was given for 21 days except those in the normal control group. Rats in model group was administered with normal saline;rats in western medicine group received 12 mg·kg-1 fluoxetine orally;rats in Shuyu Ningxin recipe low dose group, middle dose group and high-dose group orally received 2.5, 7.5, 25.0 g·kg-1 respectively. Body weight in rats on day 1 (before modeling) and 56 days (after the administration), sugar intake, the horizontal movement level and vertical movement times were compared. After the end of the experiment, the expression levels of hippocampal BDNF and TrkB were compared between various groups. Result: On day 56, the body weight of the rats, sugar intake, horizontal movement level, vertical movement times, BDNF and TrkB integral absorbance(IA) values in CA1 and CA3 were significantly lower than those in normal group (P<0.05);compared with model group, the above indicators in western medicine group and Shuyu Ningxin recipe group were significantly increased (P<0.05). Conclusion: Shuyu Ningxin recipe can change the expression levels of BDNF and TrkB in hippocampal tissues and thus play a role in antidepressant therapy.关键词：Shuyu Ningxin recipe;depression;brain-derived neurotrophic factor;tyrosine kinase B
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To using establish the hyperprolactinemia(HPRL) rat model by using modified Xiaoyao San, to observe the effect of modified Xiaoyao San on ovarian granulosa cell proliferation and cell cycle of HPRL rat in vitro, with the liver and spleen as the core, Intraperitoneal injection of metoclopramide was adopted to make the rat model of HPRL. Method: Totally 120 female SD rats were randomly divided into six groups:the blank group, the negative control group (model group), the positive control group (Bromocriptine group, 1 mg·kg-1), and the modified Xiaoyao San powder high, middle and low dose groups (55, 22.5, 13.75 g·kg-1). The intervention period was 30 days. MTT assay was used to detect the cell proliferation of cultured cells in each group at 24, 48, 72, 96 h.The change in each phase of the cell cycle was analyzed by flow cytometry and apoptotic index. Result: Compared with the model group, the model group showed significant decreases in granulose cell proliferation at 24, 48, 72, 96 h and S phase and increase in cell ratio in G0/G1 phase (P<0.05). Compared with the model group, modified Xiaoyao San powder group and Bromocriptine group showed significant reduce apoptotic index and increase in cell proliferation in S phase and decreases in cell ratio in G0/G1 phase (P<0.05). The effect of modifid Xiaoyao San high dose group was similar with the Bromocnptine group. Conclusion: Modified Xiaoyao San has a significant effect in promoting proliferation of ovarian granulose cells and reduce apoptotic index, and its mechanism may be correlated with the transformation of granulose cells to S phase from G0 period and the increase in ratio of cells in S phase.关键词：soothing liver and invigorating spleen;hyperprolactinemia;granulose cell;cell cycle
- 3
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To determine the angiogenesis effect of radix astragali extract on myocardium of rats with myocardial infarction (MI) and analyze its possible mechanism. Method: Left coronary arteries of Sprague-Dawley rats were ligated to make MI models. The rats were randomly divided into MI model group, 3 different dose groups of Astragali Radix extract (10, 20, 40 mg·kg-1·d-1), and the sham operation group. Each group consisted of 8 rats. All the treatment groups were orally fed with the drug extract of above corresponding dose, and the model group and sham operation group were fed with normal saline 20 mL·kg-1·d-1. 4 weeks later, hematoxylin and eosin staining, Masson staining, and electron microscope were taken to analyze the left ventricular myocardial tissue and vascular pathological changes in rats with MI, and immumohistochemical staining was taken to analyze the expression changes in vascular endothelial growth factors (VEGF) and CD34 proteins. Result: Compared with the sham operation group, myocardial tissue was disordered in the model group, accompanied by severe myocardial necrosis, significantly reduced quantity of complete microvessel (P<0.05), significantly increased collagen content (P<0.01), and obvious endothelial cell loss (P<0.05). Compared with the model group, the structures of myocardial tissue in all the treatment groups were relative regular, with significantly increased collagen content (P<0.01), significantly increased number of new microvessels (P<0.05), as well as relatively complete endothelial cell morphology and increased number of endothelial cells (P<0.05). In addition, VEGF and CD34 protein expression in the cytoplasm of myocardial tissue was increased significantly (P<0.01). Conclusion: Astragali Radix extract can obviously improve the disorganized myocardial tissue of rats after myocardial infarction, and promote angiogenesis in the impaired myocardial tissue.关键词：Astragali Radix;myocardial infarction;angiogenesis;pathology;tonifying Qi
- 3
- |
- 2
- |
- 5
发布时间：2024-01-04 - 摘要：Objective: To explore the regulating effects of Clausenae Lansii Folium extracts on cytoskines level of type Th1/Th2 in inflammation reaction on asthmatic rats. Method: Forty-eight healthy male rats were randomly divided into six groups:normal control group, asthma model group, dexamethasone group(1.5 mg·kg-1·d-1), Clausenae Lansii Folium extracts groups of low, medium and high doses (520, 1 040, 2 080 mg·kg-1·d-1). Except normal control group, all the other groups were sensitized with ovalbum by subcutaneous injection on the first and the seventh day. From the fifteenth day, rats in various administering groups received atomization treatment 0.5 hour after gavage 1 hour for 1 week. The normal control group and asthma model group were treated with normal saline instead. The levels of nitric oxide (NO), leukotriene D4(LTD4), interleukin (IL-4), interferon-γ (IFN-γ) in serum were measured by ELISA, and NO in lung tissue was detected by nitrate reductase method. The expression of IL-4, IFN-γ mRNA in lung tissue was measured by RT-PCR. The pathological changes in lung tissue were observed. Result: Compared with the normal group, levels of NO, IL-4 and LTD4 in serum of rats in model group were significantly higher, and IFN-γ content was lower. NO content in lung tissues was also significantly higher, IL-4 mRNA expression was significantly higher, and IFN-γ mRNA was significantly lower (P<0.01). Compared with the model group, all of Clausenae Lansii Folium groups of low, medium and high dose could significantly reduce NO and IL-4 levels in serum, and increase IFN-γ level (P<0.05, P<0.01). Clausenae Lansii Folium groups of high, medium doses could reduce the level of NO in lung tissues of the asthma rats. Clausenae Lansii Folium high dose group could significantly reduce the level of LTD4 in serum of asthma rats (P<0.05, P<0.01). All of Clausenae Lansii Folium groups of low, medium and high dose could significantly reduce IL-4 mRNA expression and increase IFN-γ mRNA expression (P<0.05, P<0.01). Conclusion: The extracts of Clausenae Lansii Folium are effective in treatment of inflammation reaction in asthmatic rats by adjusting Th1/Th2 balance, and reducing the infiltration of inflammation cells.关键词：asthmatic rat;Clausenae Lansii Folium extracts;inflammation cytoskines;Th1/Th2
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of the Zhongfeng capsule on blood fat and cytokines in rats with atherosclerosis. Method: SD rats were randomly divided into 5 groups:the normal group, the model group, the high-, low-dose Zhongfeng capsule groups and the positive group (atorvastatin calcium tablets, 5 mg·kg-1). The rats in the normal group received saline and basic food. The rest rats were injected with VD3 and ovalbumin, while fed with a high-fat diet. All rats received the corresponding drugs for 90 days. The levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C), interleukin-6 (IL-6), interleukin-18 (IL-18), tumor necrosis factor-α (TNF-α), adiponectin(APN) and soluble CD40L (sCD40L) in the blood serum were analyzed and the arteriosclerosis index (AI) were calculated. The pathological observation of aorta was carried out. Result: Compared with normal group, the levels of TC, LDL-C, HDL-C, TNF-α, IL-18, sCD40L and AI were significantly increased, while the APN decreased in the model group (P<0.05, P<0.01). Compared with the model group, the AI and LDL-C, TNF-α, IL-18 levels decreased, while the serum APN level increased in the high-dose Zhongfeng capsule group (P<0.05, P<0.01). HE stain showed aortic endothelial injury and atherosclerotic plaque formation in the model group, while the aortic lesions could be restrained in different degree in the treated groups. Conclusion: Zhongfeng capsule could inhibit the atherogenesis formation and development in atherosclerosis. The mechanisms may be involved in reducing the level of serum LDL-C, improving the level of APN and decreasing the secretion or release of the inflammatory factors.关键词：Zhongfeng capsule;atherosclerosis;cytokines
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study the effect of drug-containing serum with modified Shengmai decoction on cell proliferation, cell cycle and migration of non small cell lung carcinoma(NSCLC)H460 cells, and investigate its molecular mechanisms. Method: Drug-containing serum with modified Shengmai decoction was prepared by administering the rabbits with 18 g·kg-1 crude drug ig, and blank control serum was prepared with the same volume of normal saline(NS) ig. Both serum was regarded as a concentration of 100%. H460 cells were cultured in vitro and cells in logarithmic growth phase were collected. 10% control serum group, 2.5%, 5%, 10% drug-containing serum groups and 1 mg·L-1 DDP group were established. Cell density was adjusted to 3 000 cells/mL. After serum action for 24, 48 hours, four methyl azo blue(MTT) method was used to measure the proliferation inhibition rate. Cell density was then adjusted to 5×105 cells/mL, and after serum action for 24 hours, flow cytometry was used to test cell cycle, scratch assay was taken to observe cell migration, and Western blot method was used to measure the expression changes of E-cadherin(E-cad) and Vimentin. Result: Compared with the blank control group, drug-containing serum with modified Shengmai decoction had higher cell proliferation inhibition rate (P<0.01), higher cells percentage in S phase(P<0.01), longer wound scratch distance, higher protein content of E-cad and lower Vimentin (P<0.01) after serum action for 24 hours. Conclusion: Modified Shengmai decoction has an inhibition effect on cell H460 mainly by inhibiting cells in phase S. Modified Shengmai decoction can inhibit the metastasis of H460, whose mechanism may be associated with intervening the epithelial-mesenchymal transformation.关键词：modified Shengmai decoction;non-small-cell lung cancer;H460 cell;proliferation;cellcycle;migration
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect of anti-Candida albicans of Jingwanhong constituents of berberine hydrochloride, baicalin and borneol in vitro. Method: The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of berberine hydrochloride, baicalin, borneol (50, 100, 150 g·L-1) were determined by two-fold dilution method and checkboard method. The anti-bacterial effects of the combined three drugs were measured and the combined inhibitory fraction (FIC) was calculated. Result: The MIC of berberine hydrochloride, baicalin, borneol was 160, 1 280, 320 mg·L-1, and the MBC was 640, 20 480, 640 mg·L-1, respectively. Baicalin and berberine hydrochloride combined showed the synergistic effect with FIC index of 0.5.The FIC of baicalin and borneol combined was 0.625, which showed the additive effect.The berberine hydrochloride and borneol combined showed the synergistic effect with FIC index was 0.5.Three-drug combination had a certain synergy with the FIC index of 0.375. Conclusion: Three constituents of berberine hydrochloride, baicalin, borneol could inhibit C. albicans, and three drugs combined showed a certain synergy.关键词：Candida albicans;berberine hydrochloride;baicalin;borneol
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of Qiqilian capsule on the left ventricular hypertrophy (LVH) of spontaneously hypertensive rats (SHR) and explore the possible mechanisms. Method: Twelve-week-old SHRs were divided into the model group, the losartan group (4 mg·kg-1) and Qiqilian capsule high, middle and low dose groups (1.2, 0.6, 0.3 g·kg-1), with 10 rats in each group. Another 10 healthy WKY rats were included in the normal group and given normal saline, ig. All of treatment groups were given corresponding drugs, ig, for consecutively six weeks. The blood pressure were measured by tail-cuff, and the method of ELISA was used to determine the contents of serum tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-10 (IL-10) and high-sensitivity C-reactive protein (hs-CRP). Changes of cardiac hypertrophy parameters of SHRs were observed. Result: Compared with the normal group, the model group showed significant higher SHR blood pressure, serum TNF-α, IL-1β, hs-CRP, left ventricular hypertrophy index(LVH), left ventricular weight(LVW), right ventricular weight(RVW), myocardial fiber diameter(MD) and lower IL-10 content (P<0.01). The Qiqilian capsule high, middle-and-low-dose groups showed significantly lower blood pressure of SHRs, TNF-α, IL-1β, hs-CRP, lower IL-10 content (P<0.01) and lower LVHI, LVW/RVW, MD in SHR rats (P<0.05, P<0.01). According to the pathological detection, the model group showed diffuse enlargement, deformity, large nucleus, hyperchromic effect in myocardial cells, mild edema and disorder arrangement in myocardial fibers arranged, and significant inflammatory cell infiltration;all treatment groups showed obvious mitigations. Conclusion: Qiqilian capsule has the protective effect against LVH in SHRs, and the mechanism may be related to inhibition of the expression of inflammatory cytokines.关键词：Qiqilian capsule;left ventricle hypertrophy;myocardial tissue;inflammatory factors
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To study effect and mechanism of gallic acid which extracted and separated from Dimocarpus longan stone on melanin synthesis of B16 cells. Method: Cultured mouse melanoma B16 cells in vitro, and treated with different concentrations of gallic acid in different time.Its effect on B16 cells proliferation was observed by MTT assay, content change of B16 cells melanin was determined to observe effects of gallic acid on melanin synthesis.By morphological observation, Hoechst 33342 staining and reverse transcription polymerase chain reaction (RT-PCR) were adopted to determine inhibition ways of gallic acid on B16 cells, and changes of tyrosinase expression in B16 cells.Mechanism of gallic acid on B16 cells melanin secretion was investigated. Result: Gallic acid had inhibitory effects on B16 cells significantly at the concentration range of 1-64 mg·L-1, half inhibiting concentration (IC50) of gallic acid on B16 cells growth were 32.29, 22.47, 18.68 mg·L-1 after treated for 24, 48, 72 h, respectively.Gallic acid significantly reduced melanin synthesis in B16 cells, and had positively related with concentration and acting time in a certain range.Inhibitory effect of gallic acid on B16 cells and melanin synthesis in different ways with different concentrations.At low concentrations (≤16 mg·L-1), its inhibitory effect through apoptosis, at high concentration (>16 mg·L-1), its inhibitory effect through cell directly killing way.When the concentration of gallic acid≤32 mg·L-1, it significantly reduced expression of tyrosinase in B16 cells. Conclusion: Gallic acid has obvious inhibitory effect on B16 cells and can reduce the content of melanin in B16 cells.Gallic acid has obvious inhibitory effect on tyrosinase expression in B16 cells.关键词：gallic acid;Dimocarpus longan;whitening;molecular mechanism;apoptosis;half inhibiting concentration
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Danggui Shuangshen Hamayou tablets (DSHT) in regulating the endocrine system of ovariectomized rats. Method: Totally 50 ovariectomized female rats were randomly divided into five groups:model group, Livial group and DSHT high, middle and low dose groups (0.6, 0.3, 0.15 g·kg-1), with 10 in each group. Another 10 were included into the sham group. Each treatment group was fed with the corresponding liquid. Model group and sham group were orally given normal saline for 30 days. Animals were killed, rat brain, uterus and adrenal were collected to calculate the viscera index, and detect estradiol(E2), luteinizing hormone (LH), follicle-stimulating hormone(FSH) with content kit. Norepinephrine(NE), dopamine (DA), 5-hydroxytryptamine(5-HT) and 5-hydroxyindole acetic acid(5-HIAA) content were tested by chemical fluorescence. Result: Compared with the sham group, the model group showed significantly lower weight, serum E2, uterus index, adrenaline index, NE, DA content (P<0.05, P<0.01) and higher LH, FSH, 5-HT, 5-HIAA content (P<0.05, P<0.01). DSHT could significantly increase the weight of rats in the model group, each DSHT group could significantly increased serum E2 levels in rats (P<0.05), with no effect on LH, FSH. Each treatment group showed significantly increase in the lowered uterus index after castration (P<0.05), and high dose of DSHT could increase the lowered adrenal index after castration (P<0.05). Each group showed significantly reduction in the increased 5-HT, 5-HIAA content in ovariectomized rats (P<0.05). The high-dose and the middle-dose of DSHT could significantly elevated the reduced NE, DA levels in rats after castration (P<0.05). Conclusion: DSHT can regulate reproductive endocrine hormones and hypothalamic neurotransmitters.关键词：Danggui Shuangshen Hamayou tablets;bilateral ovariectomy;reproductive endocrine hormone;viscera index;hypothalamic neurotransmitters
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Chenyuan ointment rubbing on the serum levels of nitric oxide(NO) and prostaglandin E2(PGE2) of rats with knee osteoarthritis (KOA) and explore its anti-inflammatory effect. Method: Totally 52 SD rats were randomly divided into 4 groups:normal group (group A), model group (group B), Chenyuan ointment group (group C, 2 g/rat) and manipulation control group (group D, Vaseline, 2 g/rat), with 13 rats in each group. KOA model was induced by closed-plaster method, except for the normal group. Eight weeks after fixation, groups A and B were given no intervention, the rats in groups C and D were given Chenyuan ointment with manipulation and vaseline with manipulation once a day. After 8 weeks, HE staining was adopted to observe the pathological changes of articular cartilage in each group and Mankin score and detect the serum levels of NO and PGE2. Result: Compared with group A, group B showed significantly higher mankin score and serum NO and PGE2 (P<0.01) and lower lower limb circumference and knee joint activity (P<0.01). Groups C and D showed lower mankin score and the serum levels of NO and PGE2 than that of group B (P<0.01). Specifically, group C showed the lower serum levels of NO and PGE2 (P<0.05). After the treatment, limb circumference and activity of knee joint of rats were significantly improved in groups C and D (P<0.01), particularly group C were improved more greatly (P<0.05). Conclusion: Chenyuan ointment rubbing can reduce the concentration level of NO and PGE2 in serum of KOA rats, and better improve muscle strength of lower limbs and activity of knee joint. Moreover, it has a ertain effect in reducing joint inflammation.关键词：Chenyuan ointment rubbing;osteoarthritis of knee;inflammatory mediator;nitric oxide;prostaglandin E2
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effect of Huiru Yizeng I for treating hyperplasia of mammary glands, and screen out the best prescription by pharmacodynamic experiment. Method: Totally 120 rats were randomly divided into 15 groups (n=8). Except for the normal group, the other groups were intramuscularly injected with estradiol benzoate (0.5 mg·kg-1) for 25 days and then progesterone (5 mg·kg-1) for five more days to establish the mammary gland yperplasia model. The rats were treated with different drugs for four weeks. The serum estradiol (E2), progesterone (P), prolactin (PRL), folliclestimulating hormone (FSH), luteinizinghormone (LH) were determined by ELISA. The pathomorphological changes in mammary tissues of each group were observed under microscope. Result: Compared with the normal group, the model group showed significant increases in serum E2, P, PRL, FSH, LH content (P<0.05) and the numbers of lobules, alveoli and secretions. Compared with the model group, serum hormones of rats in each treatment values were lower (P<0.05). Compared with the other groups, group 8 showed significantly decreases in the serum levels of estradiol(P<0.05), prolactin, follicle stimulating hormone, progesterone, luteinizing hormone (P<0.05) and reduction in the numbers of lobules, alveoli and secretions. Conclusion: Compound Huiru Yizeng I could significantly relieve the disorder of serum hormones, has significant therapeutic effect on hyperplasia of mammary glands, in which Hordei Fructus Germinatus, Prunellae Spica, Scrophulariae Radix, Fritillariae Thunbergii Bulbus, Laminariae Japonicae Thallus, Ostreae Concha, Cremastrae Pseudobulbus Pleiones Pseudobulbus are combined as the optimal prescription, with the most significant rapeutic effect on hyperplasia of mammary glands in rats.关键词：Huiru Yizeng I;hyperplasia of mammary glands;hormone;pathomorphology
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of hyperin (Hyp) on concanavalin A (Con A)-induced immunological liver injury in mice, and to explore its mechanism. Method: Kunming mice were randomly divided into six groups:the normal group, the model group, the low-, middle-, high-dose Hyp groups (12.5, 25, 50 mg·kg-1) and the bifendate group (200 mg·kg-1). The mice in he treated groups were orally administered with hyperin or bifendate for 10 consecutive days. The mice were injected with 20 mg·kg-1 of Con A intravenously except the control 1 hour after the last medication. 12 hours later, the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, the concentrations of superoxide dismutase (SOD) and methane dicarboxylic aldehyde (MDA) in hepatic tissue, the levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interferon-γ (IFN-γ) and tumor necrosisfactor-α (TNF-α), CD3+, CD4+ and CD8+ in blood were determined. In addition, hepatic histopathological examination was also performed. Result: Compared with the normal group, the serum AST and ALT activities, the level of MDA, IL-2, IL-4, IFN-γ and TNF-α increased, the SOD, T lymphocyte subset CD3+, CD4+ and CD8+ ratios in hepatic tissue decreased in the model group (P<0.01). Compared with model group, the serum AST and ALT activities, the level of MDA, IL-2, IL-4, IFN-γ and TNF-α were reduced, the SOD, T lymphocyte subset CD3+, CD4+ and CD8+ ratios in hepatic tissue were elevated in the model group(P<0.01). Moreover, the pathological injuries of liver tissue were alleviated. Conclusion: Hyp has a potent protective effect against Con A-induced immunological liver injury in mice. The mechanism might be related to removing free radicals, regulating the balance of T cell subgroup and reducing the secretion of inflammation factors.关键词：hyperin;immunological liver injury;concanavalin A;inflammation factor;T cell subgroup
- 3
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：Objective: To study the relationship between the inflammation and expression of hepatic purinergic receptors P2X7 and A2A in hyperlipidemia rats, and the intervention effect of Yirui capsule. Method: Fifty male rats were randomly divided into two groups. Ten rats were fed with normal diets as normal group, and forty rats were fed with high-fat diets as the observation group. After 14 d, the forty rats were further randomly divided into model group and Yirui capsule low, middle, high dose groups, 10 rats for each group. Yirui capsules were administered ig to the treatment group for 30 days at 140, 280, 560 mg·kg-1 respectively. And then, the levels of lipids in serum were detected, tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in serum were detected by enzyme-linked immunosorbant assay(ELISA), and the expression of purinergic receptors P2X7 and A2A in livers were analyzed by RT-PCR. Result: The content of inflammatory cytokines TNF-α, IL-1β and the expression of purinergic receptors P2X7 and A2A in livers were increased in model group, compared with normal group (P<0.01). The content of inflammatory cytokines TNF-α, IL-1β and the expression of purinergic receptors P2X7 and A2A in livers were decreased in Yirui treatment group, compared with model group (P<0.01). Conclusion: Yirui capsule can regulate the expression of purinergic receptors in rat's liver, which is possibly relevant to the anti-inflammatory properties and amendment of lipid metabolism.关键词：hyperlipidemia;inflammatory;purinergic receptors;Yirui capsule
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 -
Effects of Wenyang Huazhuo Tongluo Recipe on Serum BAFF and PⅢNP in Patients with Systemic Sclerosis
摘要：Objective: To observe the effect and mechanism of Wenyang Huazhuo Tongluo recipe (WYHZTLR) in treating patients with systemic sclerosis (SSc). Method: Eighty-two patients with SSc were randomly divided into two groups. Control group (41cases) was treated with methotrexate (MTX) and prednisone. Treatment group (41cases) were treated with WYHZTLR combined with MTX and prednisone. All SSc patients were treated for six months. The serum levels of B-cell activating factor (BAFF) and aminoterminal propeptide of type Ⅲ procollagen (PⅢNP) were measured by ELISA. The levels of IgG and IgM were detected by immune scatter turbidimetry. The skin score and disease activity index were assessed at the same time. Result: The treatment group was superior to the control group in efficacy, with a statically significant difference (P<0.05). The levels of BAFF, IgG, IgM and PⅢNP in two groups after treatment were significantly lower than those of before treatment (P<0.05, P<0.01). The above indexes were improved more significantly in the treatment group compared with the control group (P<0.05). Meanwhile, skin score and disease activity index significantly decreased in two groups from that of before the treatment, particularly for the the treatment group (P<0.05, P<0.01). There were positive linear correlations between BAFF and skin score, disease activity index and PⅢNP before and after treatment (P<0.01). Conclusion: WYHZTLR can reduce skin score and disease activity index and relieve clinical symptoms by reducing the serum levels of BAFF and PⅢNP and inhibiting the immunoreaction.关键词：systemic sclerosis;Wenyang Huazhuo Tongluo recipe;B-cell activating factor;aminoterminal propeptide of type Ⅲ procollagen- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Linggui Zhugan Tang combined with Shengmai San in treating senility frequent ventricular premature beat (FVPB) as well as its influences to heart rate variability (HRV) and heart rate turbulence (HRT). Method: One hundred and eight patients with frequent ventricular premature beat were randomly divided into control group (53 cases) and observation group (55 cases) according to their order of hospitalization. Patients in control group received about 1 minute of esmolol hydrochloride intravenous injection, 0.5 mg·kg-1·min-1, with a maintenance dose of 0.05 mg·kg-1·min-1 for intravenous drip. Based on the treatment of control group, patients in observation group also received Linggui Zhugan Tang combined with Shengmai San. Courses of treatment in two groups were both 4 weeks. Before and after treatment, times of ventricular premature beat, HRV and HRT were recorded by 24 hours' ambulatory electrocardiogram. HRV included 4 indicators:24 hours' standard deviation of normal to normal RR intervals (SDNN), standard deviation of 5 min average normal RR intervals in 24 h (SDANN), root mean square successive differences (RMSSD) in normal R-R intervals, and percentage of differences between adjacent normal RR intervals exceeding 50 milliseconds, PNN50.HRT included 2 indicators:turbulence onset (TO) and turbulence slope (TS). And scores of main symptoms were also graded. Result: Total effective rate for premature beat was 92.73% in observation group, and 77.36% in control group. Thus, the efficacy in observation group was better than that of control group for comparison (P<0.05). After treatment, the SDNN, SDANN, RMSSD and PNN50 in observation group were superior to these in control group (P<0.05, P<0.01). TS in two groups went up slightly compared with the data before therapy, while the increase in observation group was more obvious (P<0.01). The number of cases with ventricular premature beat in observation group was less than that in control group (P<0.05), and the reducing number of ventricular premature beat in observation group was more than that in control group(P<0.05). After therapy, the main symptom scores in observation group were lower than these in control group (P<0.01). The decrease of main symptom scores in observation group were superior to that in control group (P<0.01). Conclusion: Linggui Zhugan Tang combined with Shengmai San for senility frequent ventricular premature beat could improve HRV and HRT, reduce the incidence of ventricular premature beat, and improve the clinical symptoms, with better clinical efficacy compared with western medicine alone.关键词：frequent ventricular premature beat;Linggui Zhugan Tang;Shengmai San;heart rate variability;heart rate turbulence
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic efficacy of Naogengtong decoction combined with Shuxuening injection on hemiplegia in recovery stage of cerebral infarction and its impact on interieukin-6(IL-6), interieukin-10 (IL-10), tumor necrosis factor (TNF-α), matrix-metalloproteinase-9 (MMP-9). Method: Eighty-eight patients of cerebral infarction were randomly divided into control group and observation group of 44 patients each by random number table. Both groups received conventional treatment. In control group, patients received Shuxuening injection (iv gtt and qd). The patients in observation group were also treated with Naogengtong decoction (1 dose/d and bid) by oral administration based on the treatment of control group. Course of treatment were 4 weeks for both groups. Barthel Index (BI), Fugl-Meyer score (FMMS), disability rate, CSS score, and improved Ashworth score were compared between two groups. IL-6, IL-10, TNF-α, and MMP-9 in serum were detected and compared between two groups. Result: The clinical efficacy in observation group was superior to that of control group by ridit analysis (P<0.05). The BI and FMMS score of upper and lower limbs in the observation group were higher than those of control group (P<0.01). After treatment, the disability rate in control group and observation group was 25.12% and 15.39% respectively, which showed no statistical significance.After treatment, CSS scores and improvement Ashworth scores in observation group were lower than those in control group (P<0.01). IL-6, IL-10, TNF-α and MMP-9 levels in observation group were lower than those in control group (P<0.01). Conclusion: Naogengtong decoction combined with Shuxuening injection can improve activity of daily living, FMMS score, muscular tension, and neurologic impairment in patients with hemiplegia in recovery stage of cerebral infarction, and its mechanism may be related with inhibiting the inflammatory response and down-regulating the level of MMP-9.关键词：Naogengtong decoction;Shuxuening injection;hemiplegia;interleukin-6;interleukin-10;tumor necrosis factor-α;matrix metalloproteinase-9
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To observe the efficacy and treatment course of Shuangyin Jiuzheng decoction combined with acupuncture in the treatment of wind-heat type peripheral facial paralysis. Method: One hundred and eight patients with wind-heat type peripheral facial paralysis were randomly divided into acupuncture group (54 cases) and treatment group (54 cases) according to the number table method. Patients in acupuncture group accepted acupuncture treatment, qd and one day interval every week. The patients in treatment group were additionally given with Shuangyin Jiuzheng decoction based on one acupuncture treatment, 3 times/day, 1 dose/day taken orally. The treatment course was 4 weeks in both groups. Facial disable index physiology(FDIP), facial disability index society(FDIS), house-branckmann(H-B) scale, and traditional Chinese medicine(TCM) syndrome were scored in both groups before and after treatment. Result: The total effective rate in treatment group was 90.74%, higher than 75.93% in acupuncture group (P<0.05). The clinical cure rate was 50% in treatment group, higher than 24.07% in acupuncture group (P<0.05). The number cured cases in treatment group was higher than that in control group at the 2nd week after treatment (P<0.05). After treatment, FDIP score in treatment group was higher than that in acupuncture group, with statistical significance between two groups (P<0.05), and FDIS score in treatment group was lower than that in acupuncture group (P<0.05). Decrease of H-B score and TCM syndrome score in treatment group was more significant than that of acupuncture group (P<0.05). Conclusion: Compared with acupuncture alone, Shuangyin Jiuzheng decoction combined with acupuncture can obviously improve the total effective rate and shorten the course of treatment in the treatment of wind-heat type peripheral facial paralysis, and deserves clinical application.关键词：peripheral facial paralysis;Wind-heat syndrome;Shuangyin Jiuzheng decoction;acupuncture
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic efficacy of Yiqi Huoxue decoction on cardiac function and life quality in patients with qi-deficiency and blood stasis syndrome in chronic heart failure (CHF), as well as its impact on cystatin C (Cys C), homocysteine(Hcy), and cyclophilin A(Cyp A) in serum. Method: One hundred and eighteen patients with CHF (Qi deficiency and blood stasis syndrome) were randomly assigned to either control group or observation group of 59 patients each according to random number table. Two groups were treated according to the guide of the diagnosis and treatment of chronic heart and failure. Patients of control group accepted hydrochlorothiazide tablet (25 mg/time, qd), captopril (12.5 mg and bid), digoxin tablet (0.25 mg and qd), and Metoprolol(25 mg, bid).The patients of observation group were treated with additional Yiqi Huoxue decoction based on control treatment, 1 dose/d and bid. Treatment course was 4 weeks in both groups. Lee's CHF score, NYHA cardiac heart grading, 6 minutes walk test (6 MWT), and clinical symptom score were compared between both groups before and after treatment. The quality of life of the two groups was evaluated by the minnesota heart failure life quality questionnaire. Left ventricular ejection fraction (LVEF) and E/A were detected using color Doppler ultrasound diagnostic system.Cys C, Hcy and Cyp A were tested in both groups. Result: Total effective rate for observation group was 93.22%, higher than 79.66% in control group (P<0.05). After treatment, 6 MWT in observation group was higher than that of control group, while scores of life quality and Chinese medicine(TCM) syndrome were obviously lower than those of control group, with significant differences between two groups (P<0.05). After treatment, LVEF and E/A were obviously increased in both group (P<0.01), and the increase value in observation group was higher than that in control group (P<0.05). After treatment, Cys C, Hcy, and Cyp A in observation group was remarkably lower than those in control group (P<0.05). Conclusion: Additional Yiqi Huoxue decoction based on the cardiotonic and diuretic therapy can improve cardiac function and life quality for CHF patients with heart qi deficiency and blood stasis, and its mechanism may be related to depressing Cys C, Hcy, and Cyp A levels.关键词：Yiqi Huoxue decoction;chronic heart failure;cardiac function;life quality;cystatin C;homocysteine;cyclophilin A
- 3
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To research the effect of Qiangyuan Shenghua decoction on lymphocyte level of patients with posttraumatic sepsis. Method: Eighty-eight patients with posttraumatic sepsis were randomly divided into treatment group and control group. Patients in control group were treated with conventional western medicine and pure water, and patients in the treatment group were treated with Qingyuan Shenghua decoction in addition to the conventional western medicine therapy. The white blood cell count (WBC), the number of neutrophils (NEU), neutrophil percentage (NEU%), total lymphocyte count (TLC), total lymphocyte percentage (TLC%) and C-reactive protein (CRP) and calcitonin original (PCT) in venous blood of all the patients were detected on the 1st, 3rd and 7th day before and after treatment, and the mortality was also observed on the 7th day after treatment. Result: The difference on WBC, TLC, TLC%, NEU, NEU%, CRP and PCT between the two groups before and after treatment was not significant. After a day's treatment, the TLC in the treatment group was significantly different from before the treatment (P<0.05). After 3 day's treatment, the WBC, NEU, NEU%, TLC and TLC%, CRP and PCT of patients in treatment and control group were significantly different from before the treatment (P<0.05), and the WBC, NEU, NEU%, TLC and TLC%, CRP and PCT in the treatment group were significantly different from that of the control group (P<0.05). After 7 day's treatment, all of the indexes of patients in treatment and control group were significantly different from before the treatment (P<0.05) and that of the control group (P<0.05). The mortality of treatment group was less than that of control group (P<0.05). Conclusion: Qiangyuan Shenghua decoction could improve the lymphocyte level and lymphocyte ration, and the prognosis was favorable.关键词：Qingyuan Shenghua decoction;sepsis;lymphocyte
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To probe into combined application value of Qili Qiangxin capsules combined with trimetazidine treatment on heart failure patients with ischemic cardiomyopathy. Method: From January 2013 to December 2013, 110 heart failure patients with ischemic cardiomyopathy were selected in our hospital. The patients were divided into study group and control group randomly. The patients in control group were treated with trimetazidine as clinical medication. The patients in study group were treated with trimetazidine combined Qili Qiangxin capsules drug treatment. The clinical treatment of the patients in two groups were compared and analyzed. Result: Compared with control group, the patients in study group after treatment, New York Heart Association(NYHA) class for the ratios of gradeⅠ and gradeⅡ were increased significantly, up to 21.82% and 67.27%, the ratios of grade Ⅲ and grade Ⅳ were decreased significantly, only 10.91% and 1.82%, the difference were significant statistically(P<0.05). Compared with control group, the patients in study group the ratio of effective was 58.18% and the total efficiency was 92.73%, there were improved significantly, while the ratio of inefficient was 7.27%, there was decreased significantly, the difference were significant statistically(P<0.05).Compared with control group, the patients in study group after treatment, the left ventricular end-diastolic volume(LVEDV) was (87.53±12.40) mL, left ventricular end-systolic volume (LVESV)was (67.01±13.12) mL, left ventricular end-diastolic diameter (LVEDD) was (18.32±2.78) mm, left ventricular end systolic pressure(LV ESP)was (33.54±5.16) mm, there were decreased significantly, the level of left ventricular ejection fraction (LVEF) was (53.86±7.15)%, there was increased significantly, the difference were significant statistically(P<0.05). Compared with control group, the patients in study group after treatment the levels of N-terminal pro-B-type natricuretic prptide(NT-proBNP) was (417.13±90.42) ng·L-1, cordiac tioponin Ⅰ(cTnI) was (0.30±0.10) μg·L-1, hypersensitive C-reactive protein(hs-CRP) was (8.97±3.85) mg·L-1, there were reduced significantly, the difference were significant statistically(P<0.05). Conclusion: Qili Qiangxin capsules combined with trimetazidine have a positive role in promoting for reversal and improvement of cardiac function, left ventricular remodeling and neuroendocrine factors in heart failure patients with ischemic cardiomyopathy. It was suitable for clinical application and promotion.关键词：ischemic cardiomyopathy;heart failure;Qili Qiangxin capsules;trimetazidine
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To study the curative effect of additional Sini Tang combined with prucalopride in treating patients with Yang deficiency type chronic constipation, in order to offer a new therapeutic schedule for improving the clinical curative effect and reduce adverse drug reactions. Method: Totally 278 cases of patients with Yang deficiency type chronic constipation treated in our hospital from October 2012 to November 2014 were taken as the research objects, and randomly divided into western medicine group and Chinese combined with western medicine group, each group was 139 cases. The western medicine group were treated by oral prucalopride, the Chinese combined with western medicine group were treated by oral prucalopride and additional Sini Tang, both groups were treated for 12 weeks.The changes of clinical symptoms, Chinese medicine symptom score and colon transmission test score of two groups before and after treatment were observed, and the adverse reactions in the process of treatment were compared and analyzed. Result: Various symptom scores of two groups after treatment were significantly lower than that before the treatment (P<0.05), and the comparison between the groups showed that the clinical symptoms and clinical symptom scores of Chinese combined with western medicine group were significantly lower than that of western medicine group (P<0.05). The colon transmission test showed that the number of normal patients of Chinese combined with western medicine group was significantly higher than that of western medicine group, with significantly fewer moderate patients (P<0.05). After the treatment, the improvement of anal canal resting pressure, anal canal maximum systolic blood pressure, the length of the anal sphincter and the continuous extrusion time of patients in Chinese combined with western medicine group were more significant than that of the western medicine group (P<0.05).After the treatment, compared with western medicine group, the cure rate of patients in Chinese combined with western medicine group was obviously increase and no efficiency patients were significantly decreased (P<0.05).During the treatment, the incidence of adverse reactions of Chinese combined with western medicine group was obviously lower than that of the control group (P<0.05), the differences were statistically significant. Conclusion: Combined therapy with additional Sini Tang in treating Yang deficiency type chronic constipation can effectively increase prucalopride's effect in relieving clinical symptoms, improve the clinical curative effect, and significantly reduce the occurrence of adverse reactions.关键词：chronic constipation;Yang deficiency type;prucalopride;Sini tang
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy of Jiannaoling tablets combined with compound Yinxing Tongmai oral liquid in treatment of vascular cognitive impairment(VCI) and its impact on inflammation response and oxidative damage. Method: Ninety patients with VCI were randomly divided into control group (45 cases) and observation group (45 cases) by number table method. Patients in control group orally took Nimodipine tablets, 20 mg/time, 1 time/day;donepezil hydrochloride tablets, 5 mg/time, 1 time/day. The patients in observation group also took Jiannaoling tablets, 4 pieces/time, 3 times/day and Yinxing Tongmai oral liquid, 10 mL/time, 3 times/day based on the treatment of control group. Treatment course was 12 weeks in both groups. Minimum mental state examination(MMSE) and Montreal cognitive assessment scale (MoCA) were taken to evaluate cognitive function.The activity of daily living (ADL) scale was used to test the quality of life, and dialectical scale of vascular dementia (DSVD) was taken once both before and after treatment to evaluate the traditional Chinese medicine syndrome. Superoxide dismutase (SOD), malondialdehyde (MDA), C-reactive protein (CRP), tumor necrosis facor α (TNF-α) and homocysteine (Hcy) were detected both before and after therapy. Result: By Ridit analysis, the cognitive effect in observation group was superior to that in control group after treatment (P<0.05). MoCA, MMS and ADL scores in observation group were higher than these in control group after treatment, while the DSVD score was lower than that in control group (P<0.01). The scores in directional force, attention and calculation force and language ability in MMSE scale in observation group were higher than the data in control group (P<0.01). CRP, Hcy, TNF-α and MDA levels in observation group were lower than those in control group, wile SOD was higher than that in control group (P<0.01). Conclusion: Based on the conventional western medicine treatment, Jiannaoling tablets combined with compound Yinxing Tongmai oral liquid can improve the cognitive function in patients with vascular cognitive impairment, reduce clinical symptoms and enhance the activities of daily living. Its mechanism may be related to reducing inflammation and oxygen free radical damages.关键词：vascular cognitive impairment;Jiannaoling tablets;Yinxing Tongmai oral liquid;inflammatory response;oxidative damage;homocysteine
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Base construction of Good Agriculture Practice for Chinese Crude Drugs is a systematic project, for blind spots and problems existing in the production, this article intends to discuss its production and quality management system, and make working proposals.Experiences in preparation process of more than one GAP base were concluded, these referred production and quality management systems in process of GAP base were combed, and these specific works were summarized and classified.Certification preparation work was divided into six major systems, including the production technology, the experimental research, the management, the quality control, the personnel training, the document file, as well as the referred department, the personnel, the work content and the relevant software and the hardware.Recommendations for system construction in process of GAP certification were madedfor learning and discussing in the peer, promoting the development of GAP career and production of safe and high quality Chinese medicinal materials.关键词：Good Agriculture Practice for Chinese Crude Drugs;production technology system;quality control system;experimental research system;base management system;training system;file system
- 3
- |
- 2
- |
- 3
发布时间：2024-01-04 - 摘要：Objective: To systematically assess the efficacy of Shenmai injection on the mortality rate of patients with acute myocardial infarction (AMI). Method: Randomized controlled trials for evaluating Shenmai injection for AMI patients with the mortality rate as an outcome were collected from electronic databases for a systematic evaluation and meta-analysis. Besides, a cumulative meta-analysis according to the year of publication was conducted, and trial sequential analysis (TSA) was also adopted to test their results. Result: Sixteen qualified trials were included. Meta-analysis and cumulative meta-analysis indicated that Shenmai injection can decrease the mortality rate of AMI patients, with RR=0.61, 99%CI (0.43, 0.85), RD=-0.06, 99%CI (-0.09, -0.03), NNT≈15.3.TSA further verified the results. Conclusion: Shenmai injection can decrease the mortality rate of patients with AMI. Due to the limitations of the study, clinical trials with rational design and strict execution shall be carried out in the future to provide more reliable evidence.关键词：Shenmai injection;acute myocardial infarction;systematic evaluation;Meta-analysis;trial sequential analysis
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：Objective: To systematically evaluate the effectiveness and safety of Zhengtian pill for migraine. Method: The randomized controlled trails(RCTs) of Zhengtian pill and conventional western medicine or placebo in treating migraine were searched in domestic and overseas databases through computer. Literature screening, information extracting and literature quality assessment were completed by 2 reviewers independently. And Revman 5.3.0 software was adopted for the meta analysis. Result: Totally 15 RCTs involving 956 patients were included. The results showed that when compared with the control group, Zhengtian pill combined with Nimodipine group was superior in the total effective rate[RR=1.44, 95%CI(1.2, 1.74), P=0.000 1], Zhengtian pill combined with Flunarizine group had a better effective rate[RR=1.21, 95%CI(1.08, 1.35), P=0.000 9] and a shorter headache duration[MD=-0.84, 95%CI(-12.03, -4.88), P<0.000 01];when compared with the aspirin group, Zhentian pill group had no statistical difference in the total effective rate[RR=1.21, 95%CI(0.99, 1.49), P=0.06];when compared with the placebo group, Zhentian pill had a better effective rate[RR=2.5, 95%CI(1.83, 3.41), P=0.000 01], shorter headache duration[MD=-1.31, 95%CI(-1.60, -1.02), P<0.000 01], lower frequency of headache[MD=-1.42, 95%CI(-1.81, -1.03), P=0.000 01], less episode days[MD=-1.25, 95%CI(-1.91, -0.60), P<0.000 01], decreased headache degree[MD=-1.11, 95%CI(-1.43, -0.79), P<0.000 01] and better conditions for the accompanied symptoms such as nausea[MD=-1.11, 95%CI(-1.27, -0.95), P<0.000 01], photophobia[MD=-0.43, 95%CI(-0.81, -0.28), P<0.000 01] and less tears[MD=-0.54, 95%CI(-0.7, -0.38), P<0.000 01]. But the existing evidences cannot verify the relations between Zhengtian pill and reported adverse events. Conclusion: The current evidences showed that Zhengtian pill is effective in treating migraine, with better effects than placebo in reducing headache degree and headache duration and relieving accompanied symptoms, and equal overall response rate with Aspirin in treating migraine. The combination of Zhengtian pill and Nimodipine or Flunarizine had a better efficacy than simple administration of western medicine. But more RCTs with higher qualities are required to confirm the reliability and extrapolation of our conclusion.关键词：Zhengtian pill;migraine;systematic evaluation;randomized controlled trail
- 3
- |
- 2
- |
- 6
发布时间：2024-01-04 - 摘要：Objective: To discuss the composition rule of clinical prescriptions for peptic ulcer. Method: Totally 99 Chinese medicine literatures for treating peptic ulcer were retrieved by CNKI database. Their clinical prescription composition data was mined by Traditional Chinese Medicine Inheritance Support System, the methods included drug frequency statistics and association rule analysis on drug combination. Result: Totally 29 herbs were used over 10 times in the 147 traditional Chinese medicine herbs in the 99 prescriptions;40 medicine pairs were got in the condition of 20 support degree;8 medicine pairs were got when degree of confidence exceeded or equaled to 0.9. Conclusion: The most commonly used drugs for treating peptic ulcer in clinic were those strengthening the spleen and replenishing Qi, soothing the liver and regulating Qi, eliminating dampness and phlegm, and clearing fire medicines;peony licorice decoction, wujisan, and sinisan are common combined prescriptions for treating peptic ulcer.关键词：peptic ulcer;data mining;association rules;Traditional Chinese Medicine Inheritance Support System
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the relations among property, flavor, meridian tropism and efficacy of Xiaochaihu Tang and other derived prescriptions through the analysis of their main added Chinese herbs. Method: With the Dictionary of Chinese Medicine·Volume Prescriptions as prescription sources, Xiaochaihu Tang and other derived prescriptions were collected. With the frequency analysis as the starting point, the golden section method was introduced to select out the main added Chinese herbs in these prescriptions and analyze their main efficacy, main property, flavor as well as meridian tropism. Result: The 509 collected prescriptions included 171 added herbs, and 25 of them were used for 1 506 times, accounting for 62.53% of all the added herbs. Among all herbs, Radix Paeoniae Alba was the most frequently used. By efficacy, antipyretic herbs were the most frequently used. By property, flavor and meridian tropism, the frequently used herbs were mostly cold and bitter and entered the spleen meridian. Conclusion: By using the frequency analysis of the data mining method, we can better study the prescription features and compatibility laws of the added Chinese herbs in Xiaochaihu Tang prescriptions, which provides theoretical guidance for the clinical herbal prescriptions.关键词：Xiaochaihu Tang;data mining;frequency analysis;property, flavor meridian, meridian tropism and efficacy;compatibiliy regularity
- 3
- |
- 2
- |
- 7
发布时间：2024-01-04 - 摘要：To elucidate scientific connotation of nature and flavour of Achyranthis Bidentatae Radix, as well as provide theoretical basis for guidingits clinical practice and scientific research.Herbal textual research onAchyranthis Bidentatae Radix from ancient herbal works and modern data analysis.Through textual research from ancient herbal literatures, we found that Achyranthis Bidentatae Radix and Cyathulae Radix used now was in accordance with the plant recorded in these ancient herbal literatures.In addition, evolution process of its nature was neutral, flavour of Achyranthis Bidentatae Radix was from bitter or sour to bitter, sour and sweet, flavour of Cyathulae Radix from bitter and sour to bitter and sweet was found, and evidence of nature and flavour of Achyranthis Bidentatae Radix and Cyathulae Radix existed was elucidated.This work can provide important reference for research on nature and flavour, development and clinical application of Achyranthis Bidentatae Radix and Cyathulae Radix.关键词：Achyranthis Bidentatae Radix;Cyathulae Radix;nature and flavour;herbal textual research;Shennong's Herbal Classic
- 3
- |
- 2
- |
- 2
发布时间：2024-01-04 - 摘要：To analyze and summarize the toxicity mechanism of Arecae Semen based on related domestic and abroad literatures on Arecae Semen these years, and provide reference for the further study of Arecae Semen. Through research, we find that chewing Arecae Semen can lead to oral submucous fibrous changes. Meanwhile, it also can cause reproductive system toxicity for people and other animal, increase risk for cirrhosis and hepatocellular carcinoma, reduce the body's immune system function, produce nervous system toxicity and has a relationship with cardiovascular and endocrine disease. In recent years, the toxic effects of Arecae Semen have drawn much attention from many scholars at home and abroad, and the research of toxicity mechanism has achieved much progress. However, the detailed mechanism of toxicity has not been clarified so far. The summary of the researches on toxicity mechanism of Arecae Semen in recent years is conductive to more scientific and healthier development and utilization of Arecae Semen.关键词：Arecae Semen;toxicity;mechanism
- 3
- |
- 2
- |
- 4
发布时间：2024-01-04 - 摘要：With the development of social productive forces and improvement of people's living standards, the treatment of perimenopausal syndrome(PMS) has attracted more and more attention. In this paper, we would summarize the animal models of PMS by analyzing the characteristics of the clinical symptoms for PMS, analyze the advantages and disadvantages of commonly used animal models of PMS based on the clinical features of the disease, propose more sophisticated clinical ideas and methods of animal models to study PMS, lay a foundation for the quantitative evaluation of animal models, promote the combining of diseases and PMS in animal models, and provide reliable and accurate experimental methods for the future study of PMS, thereby promoting in-depth study of PMS.关键词：perimenopausal syndrome;clinical disease characteristics;animal model
- 3
- |
- 2
- |
- 9
发布时间：2024-01-04 - 摘要：Pyrethrum tatsienense, a traditional Tibetan medicine, has effects in clearing heat, resisting inflammation, easing pain and treating grasserie. Domestic and foreign study literatures on raw material source, chemical constituents, pharmacological activities and quality standard of P. tatsienense were analyzed and summarized to provide scientific and theoretical basis for the general development of P. tatsienense. According to the findings, the main chemical constituents of P. tatsienense included essential oil, triterpenes, flavonoids, steroids and organic acids;the extracts of P. tatsienense had anti-inflammatory, analgesic, anti-hypoxia, anti-myocardial ischemia and hepatic protective effects. However, its pharmacodynamic material basis and action mechanism were still unclear, and the pharmacognosy and quality standard of P. tasienense were seldom studied. Therefore, efforts shall be made to standardize the name, varieties and base sources of P. tatsienense through herbalogical studies and current utilization surveys and improve the quality standard of P. tatsienense based on in-depth studies on pharmacology and pharmacodynamic material basis, in order to provide guarantee for the accurate clinical medication and effective safety of P. tatsienense.关键词：Pyrethrum tatsienense;pharmacognosy;chemical constituents;pharmacological activity;quality standard
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04 - 摘要：To summarize and analyze the clinecal study of traditional Chinese medicine(TCM) preparations of Gastrodiae Rhizoma. Through reviewing the relevant literatures for clinical application of TCM preparations of Gastrodiae Rhizoma in CIKI database, domestic and foreign clinical studies about traditional Chinese medicine preparations of Gastrodiae Rhizoma. The clinical literature of TCM preparations of Gastrodiae Rhizoma focused on Tianma pill, Tianma injection, Tianma capsule and Tianma tablets, while where were fewer research reports for other preparations, such as Tianma Rengong tablet, Tianma Dingxuanning, Tianma Fengwangjing and Tianma Yinao granule. Literature retrieval study showed that TCM preparations of Gastrodiae Rhizoma were commonly used in the treatment of vertigo, headache, rheumatic arthritis, neuralgia in clinic, with a good and exact effect, but occasionally caused allergic purpura, drug rash, allergic urticaria and other adverse reactions, which shall be given attention in clinical application. Over the years, many scholars have made a certain progress in studies for the clinical application of TCM preparations of Gastrodiae Rhizoma. This literature study can provide reference for the cognition, comprehensive utilization and further studies for the development of TCM preparations of Gastrodiae Rhizoma.关键词：Gastrodiae Rhizoma;traditional Chinese medicine preparation;clinical
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：To provide a beneficial reference for further research on pharmacological effects and clinical applications of Diankuang Mengxing Tang, this study was based on domestic and foreign literatures about Diankuang Mengxing Tang in the last decade. Diankuang Mengxing Tang was a classical prescription established by famous medical expert Wang Qingren of the Qing Dynasty. Diankuang Mengxing Tang was widely studied by many scholars in recent years, and they have made constant breakthroughs in pharmacological effects and clinical application. It was reported that it has pharmacological effects in sedation, hypnosis and anti-atherosclerosis, and was used to treat various clinically diseases, particularly in treating psychiatric disorders and neurological system diseases. Therefore, further studies on it have a promising prospect.关键词：Diankuang Mengxing Tang;pharmacological effects;clinical application
- 3
- |
- 2
- |
- 1
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0