最新刊期
卷 21 ， 期 20 ， 2015
- 摘要：Objective: To provide a reference for development of Shedan in situ forming eye gels by investigating isolated corneal penetration of muscone and salvianolic acid B in this preparation. Method: Taking physiological saline containing 1% tween-80 as release medium, Franz vertical diffusion cell was adopted to investigate effect of pH and muscone concentration for rabbit cornea permeability of muscone and salvianolic acid B.Release medium was precolumn dedvatized with 2, 4-dinitrophenylhydrazine under 60℃ for 30 min and was separated by HPLC.Cumulative release curves of muscone and salvianolic acid B were drawn, their kinetics models were established, apparent permeability coefficient and permeation rate of steady state were calculated. Result: Diffusion behavior of muscone and salvianolic acid B through cornea were in line with the zero-order kinetics.In vitro permeation of muscone was not sensitive to pH, while apparent permeability coefficient of salvianolic acid B increased with pH of Shedan gels decreased.Permeability of salvianolic acid B increased with the concentration of muscone increased.When the concentration of muscone reached 0.5%, apparent permeability coefficient and steady-state flow rate of muscone reached maximum, which were 1.530×10-6 cm·s-1 and 7.651×10-3 μg·s-1·cm-2, respectively;and those for salvianolic acid B were 1.218×10-6 cm·s-1 and 6.558×10-3 μg·s-1·cm-2, respectively. Conclusion: Muscone can promote corneal permeability of salvianolic acid B in Shedan in situ forming eye gels, and its optimum dosage is 0.5%.关键词：muscone;salvianolic acid B;in situ forming eye gels;isolated cornea;apparent permeability coefficient;permeation rate of steady state;Moschus
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发布时间：2024-01-04 - 摘要：Objective: To explore effects of different purification technologies on water extract of Glycyrrhizae Radix et Rhizoma. Method: Four kinds of technologies of ZTC 1+1 Ⅱ natural clarifying agents, chitosan and microfiltration combined with DA-201 macroporous resin respectively, in addition microfiltration-ultrafiltration were all used for purification of water extract of Glycyrrhizae Radix et Rhizoma.Retention rates of index components, physical and chemical parameters, and HPLC fingerprint were all applied to analyze applicability of different technologies. Result: Index components were reduced with combination technologies, which effectively removed macromolecules in water extract at the same time.Microfiltration technology in removing impurity was the best among the primary purifications.Similarity of purified liquid with microfiltration-ultrafiltration technology compared with original liquid was 0.886, which had minimal effect on liquid components among four purification technologies. Conclusion: Microfiltration-ultrafiltration combination technology might be a better method for separation and purification of water extract of Chinese medicines.This method avoids pollution of organic reagents on water extract of Chinese medicines, which is suitable for industrialized continuous production for easy operating and cost saving.关键词：Glycyrrhizae Radix et Rhizoma;membrane separation;macroporous resin;liquiritin;glycyrrhizic acid;ultrafiltration;microfiltration
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Bupleuri Radix by rice wine moistening to stir-baking with bran, and provide references for standardization production and application of this decoction piece. Method: Taking comprehensive score of saikosaponin a, saikosaponin d and serum gastrin concentration as index, orthogonal test was adopted to optimize processing technology with four factors, including bran amount, wine amout, baking temperature and time.HPLC was employed to determine the content of saikosaponin a and d with mobile phase of acetonitrile-water (35:65) and detection wavelength of 210 nm.Spleen-qi deficiency syndrome was induced in animals by compound methods which including rhubarb, exhaust and hunger.After 14 days of administration, serum gastrin was determined by radio immunoassay. Result: Optimal processing parameters consisted of 15% bran, 15% wine and 7 min of baking time at 100℃.The amount of wine, baking temperature and time had significantly effects on processing technology.Contents of saikosaponin a, d and serum gastrin concentration were 0.077%, 0.683% and 73.56 ng·L-1, respectively. Conclusion: This optimum processing technology is reasonable, feasible and reproducible by verification, it can provide a reference for industrial preparation of Bupleuri Radix by rice wine moistening to stir-baking with bran.关键词：Bupleuri Radix;Zhangbang;processing technology;saikosaponin A;saikosaponin D;serum gastrin
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发布时间：2024-01-04 - 摘要：Objective: To clarify swelling properties of original peach gum and improved peach gum affected by solvent, salt concentration, temperature and pH value. Method: Weighing method was adopted to determine wet weight and dry weight of original peach gum and improved peach gum before and after swelling, calculated equilibrium swelling ratio. Result: Equilibrium swelling ratio of improved peach gum was significantly higher than original peach gum in different solvents;Polarity of solvent higher, equilibrium swelling rate of original peach gum and improved peach gum was higher.Equilibrium swelling ratio of improved peach gum was the same to original peach gum in different salt concentrations, this parameter was not affected by salt concentration, but it was significantly less than water.In different temperature, the higher of temperature, equilibrium swelling ratio of original peach gum and improved peach gum was higher, and improved peach gum was superior to original peach gum.In different pH value, original peach gum easily affected by pH value, equilibrium swelling ratio of original peach gum was not affected by pH value and showed better performance. Conclusion: Swelling properties of improved peach gum is significantly better than original peach gum;swelling properties of peach gum before and after being improved is easily effect by solvent polarity and temperature, but it is not sensitive to salt concentration and pH value (original peach gum is susceptible).关键词：improved peach gum;equilibrium swelling ratio;solvent;salt concentration;temperature;pH
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发布时间：2024-01-04 - 摘要：Objective: To identify chemical components rapidly in raw and processed products of Polygalae Radix by high performance liquid chromatography-time-of-flight mass spectrometry (HPLC-TOF/MS) and discuss chemical components change in Polygalae Radix before and after being processed. Method: An HPLC method was applied with a Halo® C18 column (2.1 mm×100 mm, 2.7 μm), acetonitrile-0.1% formic acid aqueous solution as mobile phase with gradient elution.Mass spectrometry with ESI ion source and was performed in the positive ion mode to scan MS data about MS total ion chromatogram and ion peaks of raw and processed products of Polygalae Radix.Belonging each ion peak according to control product positioning, MS date and literature reference, number and intensity changes of ion peak of Polygalae Radix before and after processing were compared. Result: Twenty-one kinds of compounds were obtained from raw product of Polygalae Radix, which contained 13 kinds of oligosaccharide esters and 8 kinds of saponins.Twenty kinds of compounds were obtained from processed products of Polygalae Radix, which contained 11 kinds of oligosaccharide esters and 9 kinds of saponins.After boiled with licorice juice, molecular ion peak intensity of tenuifoliose C or tenuifoliose E, tenuifoliose K were disappeared;molecular ion peak intensity of sibiricose A5, sibiricose A6, tenuifoliside A, tenuifoliside B, tenuifoliside C, 3, 6'-disinapoly-sucrose, tenuifoliose A, tenuifoliose B or tenuifoliose D, tenuifoliose H, onjisaponin Vg, onjisaponin Wg and onjisaponin Gg were decreased significantly, and generated a new component which named as tenuifolin. Conclusion: After boiled with licorice juice, contents of part of oligosaccharide esters and a variety of saponins in Polygalae Radix are changed significantly.关键词：Polygalae Radix;processed products of Polygalae Radix;tenuifolin;HPLC-TOF/MS
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method for analyzing the content of doliroside B in total saponins from Dolichos appendiculatus roots, and to investigate intestinal absorption characteristics of doliroside B. Method: Ultimate AQ-C18 column (4.6 mm×250 mm, 5 μm) was used with a mobile phase consisted of water-acteonitrile containing 0.1% acetic acid for gradient elution at a flow rate of 0.5 mL·min-1, detection wavelength was 215 nm and an injection volume was 20 μL. Result: Good linearity was obtained over the concentration range of 60-210 mg·L-1 with the correlation coefficient of 0.999 9.The mean recoveries were between 95% and 105%.The content of doliroside B in four batches of total saponins extract from D. appendiculatus roots were all above 80%.In addition, the absorption rate constant of doliroside B in small intestine was (0.018 3±0.001 1) h-1 and cumulative absorption percentage in 5 h was (9.49±0.26)%. Conclusion: This HPLC method is simple and accurate for quality control of doliroside B.Doliroside B is badly absorbed in small intestine.关键词：doliroside B;total saponins;Dolichos appendiculatus;intestinal absorption;doliroside A
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology of total polysaccharides from Gynura divaricata stems and leaves by XDA-7 macroporous resin. Method: Significant factors affecting purification of total polysaccharides were firstly screened by Plackett-Burman test, taking retention rate of total polysaccharides and removal rate of protein as indexes, response surface analysis was adopted to optimize purification technology of total polysaccharides with flow rate, elution volume, ratio of diameter to height, sample volume, sample liquid concentration and pH as independent variables. Result: Sample volume, elution volume and flow rate were significant factors affecting purification effect of total polysaccharides. Optimum technology conditions were as follows:sample volume (G. divaricata as crude drug)-resin volume ratio of 0.93 g·g-1, washed with 4.3 BV of water as eluant, elution flow rate of 1.36 mL·min-1.Retention rate of total polysaccharides and removal rate of protein were 93.08% and 49.13%, respectively. Conclusion: XDA-7 macroporous resin has good effect for retention of total polysaccharides and removing protein, while serving as bleaching effect, which indicates that this method can well purity total polysaccharides in G. divaricata stems and leaves.关键词：Gynura divaricata;total polysaccharides;protein;retention rate;removal rate;macroporous resin
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发布时间：2024-01-04 - 摘要：Objective: To optimize separation and extraction method of lysozyme by reversed micellar solution. Method: Taking the content of lysozyme as index, orthogonal test and single factor tests were adopted to optimize extraction process with CTAB concentration in reversed micellar clusters solution, ratio of N-octane and n-hexanol, KCl solution concentration and extract liquid to being extract liquid ratio as factors. Result: Optimum extraction conditions of lysozyme were as follows:CTAB concentration of 30 mmol·L-1, ratio of N-octane and n-hexanol of 4:1, KCl concentration of 0.15 mol·L-1, extract liquid-being extracted liquid (1:2).The content of lysozyme was 1.330 g·L-1. Conclusion: Extracting lysozyme by reversed micellar solution has advantages of liquid-liquid extraction, while lysozyme is not deactivated and its solubility is higher, this technology is worthy further development.关键词：lysozyme;reversed micellar solution;single factor test;extraction process
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total iridoid glycosides from Hedyotis diffusa. Method: Spectrophotometry was adopted to determine the content of total iridoid glycosides with asperuloside as index ingredient.Taking composite score of extract yield and the content of total iridoid glycosides as index, orthogonal test was adopted to optimize extraction technology of total iridoid glycosides with ethanol concentration, material-liquid ratio, extraction time and times as factors. Result: Optimal extraction conditions were as follows:extracted twice with 8 times the amount of 90% ethanol, 2 h for per time;yield of total iridoid glycosides was 22.85%. Conclusion: This optimized extraction process is stable and reasonable, which can serve as references for industrial production of extraction of total iridoid glycosides from H. diffusa.关键词：Hedyotis diffusa;total iridoid glycosides;asperuloside;Ultraviolet spectrophotometry;extract yield
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发布时间：2024-01-04 - 摘要：Objective: To optimize percolation extraction process of Shexiang Shuhuoling, and provide a reference for process improvement of this preparation in industrial production. Method: Based on single factor test, with composite score of transferring rate of catalpol and solid-containing content as index, orthogonal experimental design was adopted to optimize percolation extraction process by taking percolation rate, immersion time, ethanol concentration and amount as factors. Result: Optimum percolation extraction process was added 15 times the amount of 50% ethanol for immersing 6 h, percolation rate of 0.25 mL·min-1.Transferring rates of hydroxy safflower yellow A and catalpol were 97.50% and 102.07% with RSD of 5.4% and 3.5%, respectively. Conclusion: This process is feasible and economical, which is suitable for industrial production of Shexiang Shuhuoling.关键词：Shexiang Shuhuoling;percolation;catalpol;hydroxy safflower yellow A
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发布时间：2024-01-04 - 摘要：Objective: To compare contents of eight medicinal components (geniposidic acid, deacetyl asperulosidic acid methyl ester, genipin 1-gentiobioside, gardenoside, crocin I, neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid) in Gardeniae Fructus from different localities, and provide a reference for selecting its optimal locality. Method: HPLC was performed on a Kromasil C18 column with mobile phases of acetonitrile-0.1% phosphoric acid solution in a gradient mode at a flow rate of 1.0 mL·min-1 and detection wavelength was 238, 324, 440 nm.Taking each component as indeses for cluster analysis, four main factors were extracted by principal component analysis in SPSS 20.0. Result: Four principal component which accounted for over 95% of the total variance, qualities and uniformities of Gardeniae Fructus from GAP bases of Zhangshu county and Taihe county were higher than others. Conclusion: Contents of medicinal components in Gardeniae Fructus from different localities are significant different.There is correlation between contents of each component and localities.关键词：Gardeniae Fructus;principal component analysis;cluster analysis;gardenoside;neochlorogenic acid;geniposidic acid
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发布时间：2024-01-04 - 摘要：Objective: To discuss the difference in volatile compositions between Zingeris Rhizoma Recens, Zingiberi Rhizoma and Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex or Zingiberi Rhizoma processed Magnoliae Officinalis Cortex. Method: GC-MS was applied to determine the volatile oil compositions and contents in Zingeris Rhizoma Recens, Zingiberi Rhizoma, raw Magnoliae Officinalis Cortex, Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex and Zingiberi Rhizoma processed Magnoliae Officinalis Cortex. Result: Total volatile oil content in Zingiberi Rhizoma was higher than that in Zingeris Rhizoma Recens. Total volatile oil content in Zingiberi Rhizoma processed Magnoliae Officinalis Cortex was also slightly higher than that in Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex. Nine components were found in Zingeris Rhizoma Recens and 38 components in Zingiberi Rhizoma, 4 in common. Sixteen components were found in raw Magnoliae Officinalis Cortex, 16 components in Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex, 17 components in Zingiberi Rhizoma processed Magnoliae Officinalis Cortex. Five components were in common among these three. Fourteen components were in common between Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex and Zingiberi Rhizoma processed Magnoliae Officinalis Cortex. In these 14 common components, the relative mass fraction of 4 components was higher in Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex than that in Zingiberi Rhizoma processed Magnoliae Officinalis Cortex, but the relative mass fraction in rest components was lower than that in Zingiberi Rhizoma processed Magnoliae Officinalis Cortex. Conclusion: There is difference in volatile oil compositions and contents between Zingeris Rhizoma Recens processed Magnoliae Officinalis Cortex and Zingiberi Rhizoma processed Magnoliae Officinalis Cortex. The results provide a better way to clarify the difference between Zingeris Rhizoma Recens and Zingiberi Rhizoma when they are considered as processing assistants.关键词：Zingeris Rhizoma Recens;Zingiberis Rhizoma;officinal magnolia bark;essential oil
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发布时间：2024-01-04 - 摘要：Objective: To develop a UPLC-QTRAP-MS/MS analysis method for the simultaneous determination of 10 nucleosides and nucleobases in Polygoni Multiflori Radix, and analyze the differences of nucleosides and nucleobases in raw and prepared Polygoni Multiflori Radix. Method: QTRAP UPLC-MS/MS technology was performed on a Waters Atlantis T3 column (2.1 mm×150 mm, 3 μm), eluted by a mobile phase of methanol-5 mmol·L-1ammonium acetate (0.1% acetic acid) at a flow rate of 0.4 mL·min-1. Ten kinds of nucleosides and nucleobases were analyzed in the positive ion multiple reaction monitoring (MRM) mode. Result: Ten nucleosides and nucleobases had good linearities, correlation coefficient > 0.99;the content of uridine, adenine, guanosine and cytidine was higher in Polygoni Multiflori Radix;there was significant difference in nucleoside compositions between raw and prepared Polygoni Multiflori Radix. Conclusion: The method is simple, sensitive, accurate, and can provide a reliable and effective technique for the quality control of Polygoni Multiflori Radix.关键词：Polygoni Multiflori Radix;QTRAP LC-MS/MS;nucleosides;nucleobases
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发布时间：2024-01-04 - 摘要：Objective: To analyze compositions of volatile oils and anti-oxidative activities of flowers, stems and leaves of Lonicera japonica collected in Shandong province. Method: The volatile oils were extracted from the flowers, leaves, stems of L. japonica, and the compositions were identified and compared by GC-MS;70% ethanol was used to extract L. japonica flowers, stems and leaves, respectively. After solvents were recovered from the extract, petroleum ether, ethyl acetate, n-butanol were respectively used for extraction. In vitro anti-oxidative activities of the extracts, extracted aqueous phases and volatile oils were compared by employing DPPH scavenging assay. Result: The 49 compositions were separated and identified from different parts of L. japonica and eleven of which were mutual in the flowers, leaves, stems, respectively accounting for 60.66%, 77.98% and 80.38% of their volatile oils. Palmitic acid represented the highest percentage in these three parts. All samples of extracts could eliminate free radicals, and the scavenging capacity increased with increasing concentration, but there was difference between various samples. Ethyl acetate extract had the strongest scavenging capacity, followed by N-butyl alcohol extract, aqueous phase, volatile oils, and petroleum ether extract in turn. Conclusion: The volatile oils of flowers, stems and leaves of the plant were highly similar to each other in compositions;all of volatile oils and different polar extracts of these three parts have good antioxidant capacity.关键词：Shandong;Lonicera japonica;flower;stem;leaf;volatile oil;anti-oxidative activity;DPPH
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发布时间：2024-01-04 - 摘要：Objective: To identify different kinds of sulfate mineral medicines by infrared spectrum and provide the reference to regulate quality control. Method: Six different kinds of sulfate mineral medicines (plaster, glauber's salt, alum, Natrii Sulfas Exsiccatus, Calcined Gypsum and Withered Alum) on Chinese Pharmacopoeia were identified with the use of FTIR, and their similarities and differences in the infrared spectrum were compared and analyzed by infrared spectrum scanning between 400 and 4 000 cm-1;infrared spectrum was measured and compared for Natrii Sulfas Exsiccatus and Sodium sulfate anhydrous. Result: Between 400 and 4 000 cm-1, infrared spectrum has similarities because all of them are sulfate mineral medicines;however, due to the differences of forming mechanism, ions, peak shape, peak number and transmittance rate, infrared can be used to identify the different kinds of sulfate mineral medicines. There was difference in derivative infrared spectrum between Natrii Sulfas Exsiccatus and Sodium sulfate anhydrous. Conclusion: The method is simple, convenient, and provides certain reference for the market supervision of mineral medicines and even traditional Chinese medicinal materials.关键词：FT;identify FTIR;Gypsum Fibrosum;Natrii Fulfas;Alumen;Natrii Sulfas Exiccatus;Cypsum Ustum;Withered Alum
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发布时间：2024-01-04 - 摘要：Objective: To study the differences of chemical composition and antibacterial activity between Zedoary Turmeric oil and dregs residual oil, and provide scientific basis for full use of Curcuma wenyujin dregs. Method: The Zedoary Turmeric oil and the dregs residual oil were extracted by hydrodistillation and separated by capillary GC-MS. The chemical compositions were determined by normalization and were identified by MS. Their antibacterial activity was determined by using micro-dilution method. Result: For Zedoary Turmeric oil, 78 chromatographic peaks were detected and 29 compounds were identified, accounting for 86.68% of the total composition. For dregs residual oil, 78 chromatographic peaks were detected and 31 compounds were identified, accounting for 70.83% of the total composition. Zedoary Turmeric oil showed inhibitory activity against Escherichia coli and Staphylococcus aureus with the same MIC value of 50.5 mg·L-1;dregs residual oil showed inhibitory activity against M. tetragenus, E. coli and S. aureus with the same MIC value of 44.4 mg·L-1. Conclusion: It is the first time to contrast the differences of chemical composition and antibacterial activity between Zedoary Turmeric oil and dregs residual oil. Chemical composition analysis shows that their main chemical ingredients are similar, including elemene, germacrone, curzerene, curdione, neocurdione etc. The antifungal activity shows that dregs residual oil is in general stronger than Zedoary Turmeric oil. The experiment scientific basis were provided for the further research and utilization of the C. wenyujin dregs.关键词：zedoary turmeric oil;dregs residual oil;antibacterial activity;hydrodistillation;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint of flavonoid in Spatholobi Caulis, and observe the difference in chemical compositions and content between dry and fresh Spatholobi Caulis from the same origin by comparing the fingerprint of them. Method: HPLC method was adopted. The determination was performed on Diamonsil® C18 column(4.6 mm×250 mm, 5 μm) with acetonitrile -0.2% phosphoric acid water solution as eluent gradient at the flow rate of 1.0 mL·min-1. The detection wavelength was 280 nm. The detection time was 80 min. Result: The common mode of HPLC fingerprint of flavonoid in dry and fresh Spatholobi Caulis was set up respectively. There were 11 common peaks in the fingerprint of dry and fresh herbs. Four of them were recognized. Conclusion: The method is simple, accurate and has a good repeatability. It can provide scientific basis for processing and utilization of Spatholobi Caulis.关键词：dry and fresh Spatholobi Caulis;flavanoid;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To select the protein extraction protocols for establishing biological fingerprint of Astragali Radix by using PAGE technology. Method: The water soluble protein of Astragali Radix was extracted by 8 different methods (concentrated gel buffer diluted by 4 times, distilled water, Tris-HCl method, the citrate method, PVP buffer, the modified saturated phenol method, TCA-acetone method and protein extract solution) and the PAGE experiment was conducted. The optimal protein extraction protocols for establishing biological fingerprint of Astragali Radix were selected according to the number and the definition of the protein bands. Result: The content of protein extracted by protein extract solution, concentrated gel buffer diluted by 4 times and Tris-HCl buffer was higher, but it showed a higher content of impurities, a dn some characteristic peaks cannot effectively isolated;the content of bands of protein extracted by TCA-acetone method, the modified saturated phenol method, PVP buffer and citrate buffer were lower, with a smaller number of bands and lower definition;bands of protein extracted by distilled water were most numerous and stabler, with a higher separation among peaks in electrophoresis fingerprint. Conclusion: The protein solution extracted with distilled water is more suitable for establishing the protein fingerprint of Astragali Radix.关键词：Astragali Radix;biological fingerprint;polyacrylamide gel electrophoresis (PAGE);protein extraction methods
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard for Xiaohelin capsules. Method: Pangolin in Xiaohelin capsules was indentified by TLC. The content of tetrahydropalmatine and rosmarinic acid were determined by HPLC. Phenomenex C18 (4.6 mm×250 mm, 5 μm) column was adopted and eluted with the mobile phase A of 0.1% phosphoric acid (adjusted pH to 6.0 with triethylamine)-methanol (45:55) and the mobile phase B of 0.1% phosphoric acid-methanol (55:45) in a gradient mode at a flow rate of 0.8 mL·min-1. The column temperature was set at 28℃. The detection wavelength was 282 nm (0-25 min, for tetrahydropalmatine) and 330nm (25.01-60 min, for rosmarinic acid). Result: The developed TLC spots were fairly clear without interference. Tetrahydropalmatine and rosmarinic acid showed a good linear relationship in the ranges between 0.015 0-0.751 0, 0.060 3-3.016 μg (r=0.999 8), the average recovery rates were 98.86%(RSD 0.9%) and 99.96%(RSD 1.0%) respectively. Conclusion: The method is simple, reliable and accurate, and can be used in the quality control of Xiaohelin capsules.关键词：Xiaohelin capsules;tetrahydropalmatine;rosmarinic acid;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To investigate the amino acids of Dendranthemae Flos with different harvest periods and processing methods to provide scientific basis for Dendranthemae Flos's quality, processing and application. Method: The majoramino acids content of Dendranthemae Flos were determinated by pre-column derivatization and RP-HPLC, the quality differences of Dendranthemae Flos with different processing methods and harvest periods were evaluated. Result: The results showed that Dendranthemae Flos contains 17 kinds of essential and non-essential amino acids, and the amino acids content of machined samples was higher than the manually processed ones;t-test indicated significant differences in content among 15 amino acids, except praline and cystine. The different processing methods had the most significant impacts on aspartic acid, glutamic acid, serine, valine and lysine (VIP>1) by partial least squares-discriminate analysis (PLS-DA). In addition, the two processing methods can be clearly distinguished by their advantages and disadvantages. No significant impact of harvest periods were detected on the nutrients according to amino acids content (P>0.05). Conclusion: The analysis of amino acids by pre-column derivatization and HPLC methods could be one of the effective methods for evaluating Dendranthemae Flos's quality. Mechanical processing can ensure stable and high quality of Dendranthemae Flos.关键词：Dendranthemae Flos;amino acids;high performance liquid chromatography;PLS-DA
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发布时间：2024-01-04 - 摘要：Objective: Through the content determination of the five kinds of chromone in Aguilariae Lignum Resinatum, to establish a method to control Aguilariae Lignum Resinatum quailty. Method: Determinate five kinds of chromone content by HPLC method, by the Waters C18 column (4.6 mm×250 mm, 5 μm), mobile phase was acetonitrile-(A)-0.1% phosphorric acid (B), flow rate was 1 mL·min-1, the detection wavelength was 252 nm, the column temperature was 30℃, the sample volume was 10 μL. Result: The linear ranges of aquilarone B, aquilarone C, aquilarone D, aquilarone E{(5S, 6R, 7R, 8S)-2-[2-(4-methoxy phenyl) ethyl]-5,6,7,8-four hydrox-y-5,6,7,8-four hydrogen chromone} were 7.15-358.00, 0.88-44.20, 1.54-76.80, 1.26-63.20, 1.54-77.00 μg respectively. The RSD of precision, reproducibility and stability tests were less than 2%, the average recoveries were ranged from 95.56% to 98.82%. Conclusion: The determination of five kinds of chromone content in Aguilariae Lignum Resinatum by HPLC method is simple and fast, effective. Established a method to determinate five kinds of chromone content in Aguilariae Lignum Resinatum successful. It is provided a reference for Aguilariae Lignum Resinatum quality control.关键词：Aguilariae Lignum Resinatum;chromone;content determination;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the lignans components in the stem bark of Styrax perkinsiae. Method: The chemical components in the stem bark of S. perkinsiae were isolated and identified by repeated chromatography on silica gel column, sephadex gel filtration chromatography and spectral methods. Result: Eight tetrahydrofuranoid lignans, one benzofuranoid lignan, and one dibenzyl butane lignan were isolated from EtOAc fraction and CHCl3 fraction of the stem bark of S. perkinsiae and identified as following:(-)-(2R, 3R)-secoisolariciresinol (1), 2R, 3S-dihydrodehydrodiconiferyl alcohol (2), matairesionol (3), (±)-salicifoliol (4), (-)-epipinoresinol (5), lariciresinol-4'-monomethy ether (6), (+)-pinoresinol monomethy ether (7), piperitol (8), styraxin (9), 1R, 2R, 5S, 6R-2-(4'-hydroxy-3'-methoxyphenyl)-6-(3", 4"-dimethoxyphenyl)-3, 7-dioxabicyclo[3.3.0]octane 4'-O-β-D-glucopyranoside (10). Conclusion: Six compounds (1, 4, 6, 7, 8, 10) were isolated from the plants of Styrax for the first time.关键词：Styrax perkinsia;tetrahydrofuranoid lignan;benzofuranoid lignan;dibenzyl butane lignan
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发布时间：2024-01-04 - 摘要：Objective: To observe the antitumor effects of Xihuang dropping pills in S180 tumor-bearing mice and their influence on angiogenesis markers (CD34) and vascular endothelial growth factor (VEGF) expression in sarcoma tissues of tumor-bearing mice. Method: S180 tumor-bearing mice were divided into model group, Xihuang pills group (2.2 g·kg-1·d-1), Xihuang dropping pills low dose, middle dose and high dose groups (1.5, 3, 6 g·kg-1·d-1) and cytoxan group (CTX, 0.02 g·kg-1). Xihuang pills group, Xihuang dropping pills low dose, middle dose and high dose groups were administered ig, and CTX group was administered ip, 1 time/2 days for continuous 10 days. The antitumor effects of Xihuang dropping pills in S180 tumor-bearing mice were observed, and the morphology changes of tumor cells were observed by HE staining. The influence of Xihuang dropping pills on CD34 and VEGF expression was observed by immunohistochemical method. Result: Compared with the model group, the body weight in tumor-bearing mice in Xihuang dropping pills high dose group was significantly increased. The anti-tumor rate in Xihuang pills group, Xihuang dropping pills low, middle, high groups and CTX group was 26.28%, 21.47%, 26.92%, 30.76% and 35.85% respectively. CD34 protein expression in CTX group and Xihuang dropping pills high dose group was significantly lower than that in model group (P<0.05, P<0.01). VEGF protein expression in Xihuang dropping pills middle, high dose group and CTX group was significantly lower than that in model group (P<0.05). There was significant difference in proteins expression between Xihuang dropping pills middle dose group and Xihuang pills group. Conclusion: Xihuang dropping pills have the anti-tumor effect in S180 tumor-bearing mice, and the mechanism may be associated with down-regulating expression of CD34 and VEGF protein in tumor tissues.关键词：Xihuang dropping pills;Xihuang pills;S180;anti-tumor;vascular endothelial growth factor;CD34
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Yishen Huazhuo decoction on the proliferation and differentiation of Alzheimer's disease (AD) neural stem cells (NSCs), and to provide experimental basis for the treatment of AD by Yishen Huazhuo decoction. Method: NSCs were isolated from Kunming rats at embryonic day (E) 14 and were processed with β-amyloid 25-35 (Aβ25-35). The cells were randomly divided into blank group, model group (5 μmol·L-1 Aβ25-35) and Aβ25-35+YSHZ decoction low dose group (0.1 mg·L-1), middle dose group (1 mg·L-1) and high dose group (10 mg·L-1).The drug was added after 2 h of Aβ25-35 action, and the cells were detected after 48 h of culture. The effect of Yishen Huazhuo decoction on the proliferation of NSCs was observed by, WST method and its effect on differentiation was determined by immune fluorescence method. Result: Compared with the blank group, the cell activity in model group was significantly reduced, with increased (GFAP+/DAPI)% and reduced (Tubulin+/DAPI)%, with significantly statistical difference (P<0.05), and the low dose group achieved the best effect. Conclusion: Yishen Huazhuo decoction promotes the proliferation of NSCs mediated by Aβ25-35, induces NSCs differentiation to neurons and inhibits their differentiation to astrocytes.关键词：Alzheimer's disease;Yishen Huazhuo decoction;neural stem cells;neuron;astrocyte
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发布时间：2024-01-04 - 摘要：Objective: To study anti-oxidation effect of drug-containing serum with Pinus yunnanensis on the damaged rat hepatocytes induced by ethanol. Method: Ten SD rats were selected and randomly divided into normal group and P. yunnanensis group (1 g·kg-1), with 5 rats in each group, twice ig daily for continuous 3 days, and the drug-containing serum was prepared 8 hours after the last ig medication. The neonatal rats were sacrificed and their normal hepatocytes were separated under sterile conditions for culture. The pre-cultured hepatocytes were divided into normal group, model group, negative group, 3 drug-containing serum groups of P. yunnanensis (25%, 50%, 100%).The normal hepatocytes were pre-treated with different concentrations of-drug-containing serum for 1 h. All the other groups except normal group and negative group received ethanol (100 mmol·L-1) for wound inducing. 8 h after injury, MTT method was used to measure cellular survival rate, and the levels of alanine aminotransferase (ALT), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) in culture medium were measured. Result: Compared with the normal group, the hepatocytes in injured rats had significantly higher survival rate, significantly reduced ALT activity and MDA content in culture medium, and significantly increased SOD activity and GSH content (P<0.05, P<0.01) after treatment with 5% and 10% drug-containing serum. Conclusion: The drug-containing serum with P. yunnanensis has anti-oxidation effect on the damaged rat hepatocytes induced by ethanol.关键词：Pinus yunnanensis;medicated serum;rat hepatocytes;anti-oxidation effect
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发布时间：2024-01-04 - 摘要：Objective: To analyze the effective protein of bone mesenchymal stem cells (BMSCs) intervened by active principle region of Yangxin Tongmai formula (apr-YTF) using the proteomics technology of isobaric tags for relative and absolute quantitation (iTRAQ). Method: SD rats received apr-YTF 31.08 g·kg-1 for 5 days and used the drug-containing serum in rats with equivalent normal saline as blank control group. 5 days later, drug-containing serum was taken to intervene BMSCs, then cell membrane proteins were extracted, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to screen and identify the differentially expressed proteins, and bioinformatics analysis was conducted. Result: The 236 proteins and 62 differentially expressed proteins were identified, including 48 up-regulating proteins and 14 down-regulating proteins. These proteins are mainly involved in 102 kinds of biological processes, 35 kinds of cell components, 6 kinds of molecular pathways and 3 signal transduction pathways. These proteins interact in the three signal transduction pathways. Conclusion: The following proteins and signal transduction pathways play an important role in the process of apr-YTF to induce BMSCs differentiation into cardiomyocytes:presenilin-1, presenilin-2 in the Notch signaling pathway, syntaxin-4 protein in soluble N-ethyl maleimide sensitive fusion protein attachment protein (SNARE), and mitogen-activated protein kinase 12 (MAPK 12) in the MAPK signaling pathway.关键词：active principle region of Yangxin Tongmai formula;bone marrow mesenchymal stem cells;isobaric tags for relative and absolute quantitation;proteomics;bioinformatics;signal transduction pathway
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发布时间：2024-01-04 - 摘要：Objective: To observe the intervention effect and mechanism of compatibility of salvianolic acid B, C on renal interstitial fibrosis in rats induced by unilateral ureteral obstruction(UUO). Method: Fiftyhealthy SD male rats were randomly divided into 5 groups:normal group, model group, salvianolic acid B group (12.5 mg·kg-1), salvianolic acid C group (12.5 mg·kg-1), salvianolic acid B+C group (6.25 mg·kg-1+6.25 mg·kg-1). Except the normal group, UUO rats models were established. Relevant treated groups were given with relevant drugs for 14 d since day 2 of modeling. Enzyme-linked immunosorbant assay(ELISA) method was used to detect β2-microglobulin(β2-MG) and N-acetyl-β-D-glucosaminidase(NAG). Type I collagen (ColⅠ), type Ⅲ collagen (Col Ⅲ) and α-smooth muscle actin (α-SMA) by immunohistochemistry staining. Result: ①Renal tubular functional results of rats in each group:compared with normal group, β2-MG and NAG levels were significantly increased in model group and various treatment groups(P<0.05); compared with the model group, the levels of β2-MG and NAG in various treatment groups were significantly decreased (P<0.05). ②Results of immunohistochemistry in nephridial tissue:the levels of ColⅠ, Col Ⅲ, α-SMA in model group were significantly higher than those in the normal group (P<0.05). The levels of ColⅠ, Col Ⅲ in salvianolic acid B, B+C group and α-SMA in treatment groups were significantly lower than those in model group (P<0.05). The levels of ColⅠin salvianolic acid B group, and B+C group were significantly lower than those in salvianolic acid C group (P<0.05). The levels of Col Ⅲ, α-SMA in salvianolic acid B+C group were significantly lower than those in salvianolic acid C group (P<0.05). Conclusion: Compatibility of salvianolicacid B, C may protect renal function, improve tubular function to a certain degree. The mechanism may interfere with UUO rats kidney with the secretion of ColⅠ, Col Ⅲ and α-SMA.关键词：renal interstitial fibrosis;unilateral ureteral obstruction model;type I collagen;type Ⅲ collagen;α-smooth muscle actin
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发布时间：2024-01-04 - 摘要：Objective: To explore the neuroprotective effects of Ginkgo terpene lactones meglumine injection (GTLMI) and its active compounds on behavior and cerebrospinal fluid in rats with cerebral ischemia/reprerfusion injury. Method: The rats were divided into six groups which included control group, model group, GTLMI group (G, 20 mg·kg-1), ginkgolide B group (GB, 20 mg·kg-1), ginkgolide A group (GA, 20 mg·kg-1) and ginkgolide K group (GK, 20 mg·kg-1). After treatment with corresponding drugs, rats in each group except control group were subjected to transient focal cerebral ischemia produced by middle cerebral artery occlusion. Neurological deficit scores and biomarkers [Ca2+, glutamate(Glu), aspartic acid(Asp), lactate dehydrogenase(LDH) and creatine phosphorinase (CK)] in cerebrospinal fluid were detected after ischemia/reperfusion. Result: Compared with control group, rats in model group showed severe brain damage with significant changes in cerebrospinal fluid, including decreased content of Ca2+ and increased levels of Glu, Asp, LDH, CK. Diterpene ginkgolides (G, GB, GA and GK) significantly improved the animal behavior caused by cerebral ischemia after 24 h (P<0.05, P<0.01). Diterpene ginkgolides also markedly increased the content of Ca2+ in cerebrospinal fluid. Meanwhile, they decreased the content of neurotransmitter Glu and Asp when compared with model group (P<0.05, P<0.01). Additionally, the content of CK in cerebrospinal fluid significantly decreased in GTLMI and ginkgolide A group compared with that in model group (P<0.05), and the content of LDH in cerebrospinal fluid significantly decreased in ginkgolide B group compared with that in model group (P<0.05). Conclusion: GTLMI and its active components could obviously improve neurologic impairment, decrease free Ca2+ inflow into the cells and the content of excitatory amino acid in cerebrospinal fluid, and improve the level of biochemical index of cerebrospinal fluid, suggesting that diterpene ginkgolides are effective agents for cerebral protection.关键词：diterpene ginkgolides;cerebral ischemia/reperfusion;glutamate;aspartic acid;Ca2+;creatine hosphokinase;lactate dehydrogenase
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发布时间：2024-01-04 - 摘要：Objective: To observe the protect effects of Baogan granules on acute chemical liver injury of mice induced by carbon tetrachloride (CCl4). Method: Ninety Kunming mice were randomly divided into normal group, model group, hugan tablet group(0.7 g·kg-1), baogan granules high, middle, low dose group(12.9, 6.45, 3.225 g·kg-1). Mices were given medicine once a day continuing 30 days. 1h after the last administration, in addition to the normal l group, each group respectively ip 0.1%CCl4 10 mL·kg-1, acute chemical liver injury in mice model was established. 24 h after death mice, collect liver tissue and serum specimens, detection of serum glutamic acid transaminase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) activity;determination of liver tissue total superoxide dismutase (T-SOD) activity, reduced glutathione (GSH) and malondialdehyde (MDA) content and hepatic histopathological examination. Result: Baogan granules can significantly reduce liver damage ALT, AST and LDH level in mice (P<0.05), Increase the T-SOD activity and GSH content (P<0.05), Reduce the MDA content in liver homogenate (P<0.05), and show concentration-response relationship;Greatly improve the pathology of liver tissue injury. Conclusion: Baogan granules on CCl4 induced acute chemical liver injury in mice has antioxidant and protect liver function function.关键词：Baogan granules;acute chemical liver injury;superoxide dismutase;malondialdehyde;liver function
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发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-proliferation and apoptosis effects of aloe emodin (AE) alone and in combination with cisplatin (AE-CP) on breast cancer cell MDA-MB-231. Method: MDA-MB-231 cells were treated for 48 h with 5, 10, 20, 30, 40, 50 μmol·L-1 AE, 2, 4, 8, 12, 24, 36 μmol·L-1 CP or various concentrations of AE-CP. Cell proliferation was detected by MTT. Apoptosis and cell cycle were detected by Annexin V FITC/PI double-staining and flow cytometer. The protein levels of Bax, Bcl-2 and Bcl-xL were analyzed by Western blot. Result: AE alone significantly inhibited cell proliferation, with an IC50 of 22.21 μmol·L-1. IC50 decreased to 9.51 μmol·L-1 when it was used in combination with CP. The combination index CI50<1, indicating a synergic effect. AE alone arrested cell cycle in G1 phase and induced apoptosis. The arrest was accentuated when CP was given simultaneously, accompanied by a marked increment of apoptosis rate. AE-CP significantly inhibited the protein levels of Bcl-2 and Bcl-xL, but increased Bax. Conclusion: AE could inhibit the cell proliferation of MDA-MB-231, arrest the cell cycle in G1 phase, and induce apoptosis by increasing apoptosis proteins and decreasing anti-apoptosis proteins. AE shows a synergic effect of apoptosis when combined with CP.关键词：aloe emodin;breast cancer;MDA-MB-231 cell;apoptosis;cisplatin
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发布时间：2024-01-04 - 摘要：Objective: To preliminarily screen out the pharmacodynamic material basis of Banxia Xiexin Tang in the treatment of gastritis, and establish the content determination method for the pharmacodynamic material basis. Method: Macroporous adsorption resin chromatography (eluting solvent:water, 30% ethanol, 70% ethanol) was adopted to separate three parts of Banxia Xiexin Tang liquid. Totally 48 healthy Wistar rats (half male and half female) were randomly divided into six groups:the normal group, the model group, the Banxia Xiexin Tang group, the water elution group (the distilled water group for short), the 30% ethanol elution group (the 30% group for short) and the 70% ethanol elution group (the 70% group for short). Except for the normal group, the experimental animal model of acute gastritis was established with absolute ethyl alcohol with the dosage of 5 mL·kg-1, ig. In addition, 60 healthy Wistar rats (half male and half female) were randomly divided into six groups, with 10 in each group, the same as above. Except for the normal group, the remaining five groups were included in the chronic gastritis model by using the comprehensive modeling method, in order to compare the therapeutic effect of the separate parts in treating experimental acute and chronic gastritis. Result: A better therapeutic effect in treatment of gastritis was found in 70% ethanol elution liquid;the gastric mucosal surface was pale pink and smooth, with no obvious ulcer, with a significant difference in ulcer inhibition rate in comparison with the model group (P<0.05). The content of baicalin and berberine hydrochloride were determined by HPLC method in 70% ethanol elution parts. Conclusion: The efficacy of active substance of Banxia Xiexin Tang mostly existed in the 70% ethanol elution parts separated by macroporous adsorption resin, its active component for treating gastritis are related to alkaloids, flavonoids.关键词：Banxia Xiexin Tang;chronic gastritis;pharmacodynamic substance foundation;baicalin;berberine hydrochloride
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发布时间：2024-01-04 - 摘要：Objective: To compare the efficacy of Wushe Qufeng decoction and its derivative prescriptions in chronic eczema of guinea pigs. Method: Eight guinea pigs (4 female and 4 male) were randomly selected from 58 healthy guinea pigs as the normal group. The remaining 50 guinea pigs were used to make the chronic eczema models by irritating the skins of their shaved backs with low-dose 2, 4-dinitrochlorobenzene as the antigen for repeated stimulation. 48 successfully modeled ones were randomly divide into 6 groups:No.1 prescription group (Wushe Qufeng decoction, 8.05 g·kg-1), No.2 prescription group(7.22 g·kg-1), No.3 prescription group(11.75 g·kg-1), No.4 prescription group(12.86 g·kg-1), hydrocortisone butyrate cream group (0.5 g·kg-1), and model group. The guinea pigs in these groups were given orally for 7 days. 24 h after the last administering, all experiment animals were sacrificed, and the efficacy was assessed according to the symptom scores in local skin lesions and pathological changes of the skin lesions. Result: Symptom score:compared with normal group, the pathological symptom scores in model group were significantly higher (P<0.05). Compared with model group, the pathological symptom scores in No.1 prescription group, No.2 prescription group, No.3 prescription group, No.4 prescription group and hydrocortisone butyrate cream group were significantly reduced (P<0.05). The pathological changes in skin lesions were significantly improved in Wushe Qufeng decoction group and hydrocortisone butyrate cream group, similar to those in drug control group. No obvious difference was found between the groups of the derivative prescriptions of the Wushe Qufeng decoction and the model group. Conclusion: The efficacy of the Wushe Qufeng decoction in the chronic eczema of guinea pigs is better than that of its derivative prescriptions, equaling to that of the drug control group.关键词：Wushe Qufeng decoction;chronic eczema;guinea pigs
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Shugan Xiaozhi decoction on blood lipid indicators of non-alcoholic steatohepatitis rats. Method: Sixty Wistar male rats were randomly divided into control group, hyper-lipidemia model group, fenofibrate group (0.1 g·kg-1·d-1), Shugan Xiaozhi decoction high dose group, medium dose group and low dose group (high, medium and low doses refer to four times, two times and one time equivalent to the adult dose). All other groups except the control group were given with high fat diet to copy non-alcoholic steatohepatitis rats model, and received corresponding medicine at the same time, but the control group and hyper-lipidemia model group received distilled water. After 8 weeks, blood lipid cholesterol (TC), triglyceride (TG), free fatty acid (FFA), liver function alanine transaminase (ALT), aspertate aminotransferase (AST) and blood rheology were detected. Result: Compared with the model group, the levels of following indicators were obviously decreased in Shugan Xiaozhi decoction high dose group, medium dose group and low dose group:TG, TC, FFA, whole blood viscosity (low shear), plasma viscosity, red blood cell aggregation index, ALT, and AST (P<0.01). Conclusion: Shugan Xiaozhi decoction has the effect of lowering blood lipid levels, protecting liver and diminishing enzymes in non-alcoholic steatohepatitis rats.关键词：Shugan Xiaozhi decoction;non-alcoholic steatohepatitis;lipid-lowering;liver-protecting
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发布时间：2024-01-04 - 摘要：Objective: To observe the toxic reactions and organ injuries in wistar rats induced by modified Zexie Tang, determinate the dose of toxic reactions and provide reference for proposing the safe dosage in human beings. Method: Eighty Wistar rats were randomly divided into normal group and modified Zexie Tang low, middle and high dose groups (15, 30, 60 g·kg-1), with 20 rats in each group, half male and half female. Rats were administered ig with this medicine once a day for 90 consecutive days. The animal signs were observed and recorded every day. Animal blood test and serum biochemical test were conducted at the end of administration and two weeks after drug withdrawal. The main organs of rats were weighed, organ index was calculated and histopathological examination was taken. Result: Modified Zexie Tang had no effect on organ index. Rats in high dose group had decreased activity, and their hair became yellow and slipping.Body weight of female rats in middle dose group and low dose group in 9th week and 10th week was significantly lower than that in control group (P<0.01, P<0.05). Body weight of female rats in high dose group in 10th week was significantly lower than that in control group (P<0.05).Histopathological examination showed that edema, necrosis and falling off in renal tubular epithelial cells occurred in 1 male rat and 1 female rat in modified Zexie tang high dose group, and liver congestion occurred in one male rat. Conclusion: No obvious harmful effects were observed in rats of modified Zexie Tang middle dose group after 90 days of medication, but high dose group may have certain liver and kidney injury. Therefore, we shall pay close attention to the risks for liver and kidney toxicity in application of long-term and large dose of modified Zexie Tang.关键词：modified Zexie Tang;long-term toxicity;toxicity of kidney and liver
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发布时间：2024-01-04 - 摘要：Objective: To explore the medication rules of Bruceae Fructus oil for anti-tumor effect and provide some experimental basis for clinical treatment of cancer. Method: Seventy-two Kunming mice, half male and half female, were divided into 6 groups:normal group, model group, cyclophosphamide group (20 mg·kg-1), Bruceae Fructus oil 6 am administration group, 2 pm administration group, and 10 pm ministration group. Bruceae Fructus oil groups received oil emulsion 5 mL·kg-1. All other groups except normal group prepared hepatoma H22 tumor-bearing mice as animal models. After 10 d of continuous oral administration, blood samples were taken from eyeball and serum was separated to determine the content of alanine amino transferase(ALT), aspartate amino transferase(AST), total protein(TP), urea nitrogen(UNB), serum creatinine(SCr) and other liver and kidney evaluation indexes. Mice were sacrificed by cervical dislocation, and their tumors, thymus, spleen, kidney, and liver were isolated for weighing. The tumor inhibitory rate and organ index were calculated then, and light microscopy was used to observe histopathological changes of sarcoma tissues. Result: Compared with model group, Bruceae Fructus oil 6 am, 2 pm, and 10 pm groups could significantly inhibit tumor weight (P<0.01) and its inhibition rate was 34.98%, 39.92% and 42.80% respectively, and could significantly improve the liver, kidney, spleen, and thymus indexes (P<0.01). 2 pm, 10 pm administration groups achieved best results, and could reduce the content of ALT, AST, TP, BUN, SCr, wherein 10 pm administration group could most reduce ALT, TP, and SCr (P<0.05, P<0.01), and 2 pm administration group could most reduce AST, BUN (P<0.05, P<0.01). The histopathological results showed that the model group had larger necrosis area for sarcoma tissues, and three treatment groups can reduce necrosis area of sarcoma cells, with 10 pm administration group most obvious. Conclusion: Bruceae Fructus oil has some anti-tumor effect and protects the liver and kidney function, and its efficacy and side effects were affected by administration time. 10 pm administration group has the best results and fewer side effects.关键词：Bruceae Fructus oil;H22 tumor-bearing mice;anti-tumor;chronopharmacology
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Bushen Huoxue granule (BSHXG) on osteoporosis model induced by retinoic acid in rats, and to explore its mechanism of action. Method: Forty-eight SD rats were randomly divided into normal group, model group, BSHXG low, middle and high dose groups, and Xianling Gubao (XLGB) group. All other groups except the normal group were orally administered with retinoic acid daily 70 mg·kg-1, continuous for 14 d. Modeling and administration were done at the same time. The low, middle and high dose groups were given with BSHXG 10, 20, 40 g·kg-1;XLGB group was given with XLGB liquid 0.3 g·kg-1;model group and normal group were administered with equal volume of normal saline. After 4 weeks of dosing, orbital blood was taken to measure the bone alkaline phosphatase (BALP), bone gamma-carboxyglutamic-acid-containing proteins (BGP), and tartrate-resistant acid phosphatase (TRACP-5β) in serum;after the animals were sacrificed, left femur was taken to make tissue pathological slices and right femur was taken to measure bone mineral density. Result: Compared with the normal group, the body weight in the rats of model group increased slowly (P<0.05);the serum levels of BALP, TRACP-5β increased significantly (P<0.01);BGP decreased significantly (P<0.05);and bone density was significantly decreased (P<0.05). Compared with model group, the body weight in various administration groups increased rapidly (P<0.05) and BALP was significantly decreased (P<0.01). BSHXG middle and high dose groups and XLGB group could significantly increase BGP (P<0.05), decrease TRACP-5β (P<0.01), and significantly increase bone mineral density (P<0.05). Conclusion: Bushen Huoxue granule has the effect in preventing and treating osteoporosis, regulating the disorder state of bone formation and bone destruction, improving bone microstructure, and increasing bone mineral density and bone turnover rate.关键词：Bushen Huoxue granule;retinoic acid;osteoporosis;bone mineral density
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of mussaendoside U on M cholinergic innervation organs (ileum smooth muscle, pupil and salivary gland). Method: ①Muscle contraction force information was obtained using muscle force measuring device in vitro. Atropine was added to bath to achieve a final concentration of 10 mg·L-1, or mussaendoside U was added in bath to achieve a final concentration of 100, 50, 25 mg·L-1 respectively. After 10 min, acetylcholine bromide was cumulatively added to observed the effect of drug on contraction force of muscle induced by different final concentration of acetylcholine bromide. The experiment was repeated for 10 times. ②Mice were divided into normal group and 5 model groups, with 10 mice in each group. Model group received intraperitoneal injection of pilocarpine once at a dose of 20 mg·kg-1to make M cholinergic nerve excitement model. After 15 min, modeled mice received intravenous injection of atropine at a dose of 2.5 mg·kg-1, or mussaendoside U at a dose of 10, 5, 2.5 mg·kg-1, and received telecarbon suspension ig at the same time. Telecarbon propulsion rate at intestine was measured after 30 min. ③Methods of mice grouping, modeling and treatment were identical with those in above.Pupil diameter and salivary gland secretion were measured respectively 15, 60, 240 min after administration. Result: Mussaendoside U reduced contraction force of intestine smooth muscle;dose-effect curve between acetylcholine bromide and muscle contraction force was shifted to right;telecarbon propulsion rate at intestine in mussaendoside U (5 mg·kg-1) group was (64.20±10.14)%, lower than that in model group;pupil diameters were (2.5±0.19), (2.2±0.18), (2.1±0.40) mm respectively 15, 60, 240 min after administration, bigger than those in model group;salivary gland secretion was (102.3±13.6), (90.2±20.5), (68.0±21.5) mg, lower than those in model group (P<0.05). Conclusion: Mussaendoside U may inhibit M cholinergic nerve excitement as a kind of anticholinergic drug.关键词：mussaendoside U;M cholinergic nerve;smooth muscle;pupil;salivary gland
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Chaihu Shugan San (CHSGS) on serum motilin (MOT) and interstitial cells of Cajal-deep muscular plexus (ICC-DMP) in model rats with liver depression syndrome. Method: All 50 SD rats were randomly divided into 5 groups:normal group, model group, the high and low dose groups of CHSGS(21.0, 10.5 g·kg-1, ig), and domperidone group (10 mg·kg-1, ig), 10 rats in each group. The rats in rest 4 groups except normal group received modeling for 3 weeks by chronic unpredicted mild stress (CUMS). In the next 2 weeks after stimulus, each group was treated with gavage intervention daily 10 mL·kg-1. The normal and the model group received ig normal saline(NS) of the same volume. The changes of weight and ethological indexes of immobility time in forced swimming test were observed before and after the administration of CHSGS. Serum MOT levels were detected by using enzyme linked immunosorbent assay (ELISA), and ICC-DMP changes were observed under electron microscopy. Result: Compared with the normal group, the rats in model group had an obvious weight loss after modeling;immobility time was prolonged (P<0.05);serum MOT levels were decreased (P<0.05). After administration, compared with model group, CHSGS could reduce the immobility time in forced swimming test (P<0.05), significantly increase serum MOT levels (P<0.05), and significantly improve the structure and function of ICC-DMP under electron microscope. Conclusion: CHSGS has the effect of promoting gastrointestinal motility, and the mechanism may be associated with regulating serum MOT levels, as well as quantity and function of ICC-DMP in rats with liver depression syndrome.关键词：Chaihu Shugan San;chronic unpredicted mild stress;liver depression;motilin;interstitial cells of Cajal
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Erdong Gao on tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and aquaporin-5(AQP-5) in rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS). Method: Seventy-two SD rats were randomly divided six groups:normal group (A), model group (B), Erdong Gao high-dose group (C), Erdong Gao medium-dose group (D), Erdong Gao low-dose group (E) and dexamethasone group (F), twelve rats in each group. All the other groups except normal group received disposably iv LPS 6 mg·kg-1 through tail vein to establish ALI rat model. The rat's received first medication 0.5 hour after modeling, once a day, lasting for 4 days. The rats of groups C, D, E were respectively ig given with Erdong Gao (10, 5, 2.5 g·kg-1);the rats of groups A and B were given with ip 10 mL·kg-1 normal saline;the rats of group F were given with ip 0.01 g·kg-1 dexamethasone. After 4 days, the rats were sacrificed, and serum and lung tissue were taken for testing. The contents of TNF-α, IL-6 in serum were detected by double antibody clip enzym-linked immunosorban assay (ABC-ELISA) method. The expression of AQP-5 in lung tissue was determined by Western bloting method. The pathological morphology of lung tissue was observed by hematoxylin and eosin(HE) staining. Result: Compared with group A, the contents of TNF-α, IL-6 in serum were obviously increased (P<0.01);expression of AQP-5 in lung tissue was significantly decreased (P<0.01) and the pathological inflammation in lung tissue became serious in rats of group B. Compared with group B, Erdong Gao could significantly reduce the content of TNF-α, IL-6 in serum (P<0.05, P<0.01), increase the expression of AQP-5 in lung tissue(P<0.05, P<0.01), and alleviate the pathological inflammation in lung tissue. Conclusion: Erdong Gao could reduce the content of proinflammatory factor TNF-α, IL-6 and increase the expression of AQP-5 in lung tissue, improve the pathological inflammation in lung tissue, and alleviate the state of pulmonary edema, thus effectively preventing and treating ALI.关键词：Erdong Gao;lipopolysaccharide;acute lung injury;tumour necrosis factor-α;interleukin-6;aquaporin-5
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Danhong injection in combination with clopidogrel in the treatment of patients with unstable angina pectoris (UAP) and its effects on level of blood lipids and inflammatory factors. Method: The 142 cases of eligible UAP patients treated in our hospital were randomly divided into the control group (n=72) and the observation group (n=70). Besides conventional treatments, the control group was also treated with clopidogrel, while the observation group was also treated with Danhong injection combined with clopidogrel. Then, the curative efficacy, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6) and adverse reactions of the two groups were compared. Result: The observation group had a therapeutic efficiency ratio of 95.7%, which was significantly higher than that of 84.7% in the control group (P<0.05). The observation group had a therapeutic efficiency ratio of 92.9% on blood stagnaton type, which was significantly higher than that of 73.6% in the control group (P<0.05). Compared with the control group, after the treatment, levels of total cholesterol [TC, (4.60±0.24) VS (5.12±0.32) mmol·L-1], triglyceride [TG, (1.82±0.32) VS (2.41±0.27) mmol·L-1], low density lipoprotein cholesterol, [LDL-C, (2.61±0.34) VS (3.36±0.33) mmol·L-1] were respectively statistically lower and levels of density lipoprotein cholestero[HDL-C, (1.71±0.22) VS (1.35±0.23) mmol·L-1] was statistically higher in the observation group (P<0.05). As for inflammatory factors, levels of tumor necrosis factor-α [TNF-α, (54.21±9.57) VS (65.34±9.27) ng·L-1], interleukin-1 [IL-1, (0.28±0.09) VS (0.45±0.11) ng·L-1] and IL-6 [(6.28±3.24) VS (9.55±3.64) ng·L-1] were statistically lower in the observation group than those in the control group after the treatment (P<0.05). During the treatment, there was no case of severe adverse reaction. The incidence of adverse reaction in the observation group was 8.6%, which was statistically the same with that of 11.1% in the control group. Conclusion: The combined administration of Danhong injection with clopidogrel for treating UAP is effective, which can significantly increase the curative efficiency, improve level of blood lipids and alleviate inflammatory reaction with minor adverse reaction, and is worth further studing in clinic.关键词：Danhong injection;clopidogrel;unstable angina pectoris;blood lipids;tumor necrosis factor-α;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: To study the clinical application value of oral administration of Miao medicine Zhubi Tang combined with external application of Miao medicine Waifu San in the treatment of gouty arthritis. Method: Eighty cases of patients with acute gouty arthritis diagnosed and treated in our hospital from January 2014 to December 2014 were selected and divided randomly into control group (40 cases) and observation group (40 cases) according to their visiting sequence. The control group was treated with traditional western medicine colchicines and diclofenac sodium, and the observation group was treated with oral administration of Miao medicine Zhubi Tang combined with external application of Miao medicine Waifu San. The various clinical indicators of two groups of patients were observed, and the statistical software was used for data processing and analysis. Result: The treatment efficiency (92.31%) of observation group was significantly higher than that of the control group (83.78%), the inter-group difference was statistically significant (P<0.05). The pain score of observation group was significantly lower than that of control group after treatment (P<0.05), the pain relief time and of swelling disappearance time were significantly shorter than those of control group patients (P<0.05) and statistically significant. The blood uric acid (UA), blood sedimentation (ESR), C-reactive protein (CRP) levels of observation group after treatment were significantly lower than those of the control group (P<0.05), the improvement degree of hemodynamic indexes of observation group was better than that of control group (P<0.05), the above indexes differences were statistically significant. The incidence of adverse reactions of observation group was lower than that of control group (P<0.05) and statistically significant. However, the differences of inflammatory cells index, blood routine, liver and kidney function between two groups were not obvious, with no statistical significance. Conclusion: Oral administration of Miao medicine Zhubi Tang combined with external application of Miao medicine Waifu San can rapidly relieve swelling and pain, reduce the UA, ESR and CRP levels, decrease the damage to peripheral blood and the incidence of adverse reactions, and improve the clinical curative effect in the treatment of gouty arthritis, and so is worth promoting.关键词：Miao medicine Zhubi Tang;Miao medicine Waifu San;gouty arthritis
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发布时间：2024-01-04 - 摘要：Objective: To discuss the curative effect of Yangxin decoction in treating sub health insomnia and influence to quality of life. Method: One hundred and fourteen patients with sub health insomnia were randomly divided into control group (71 cases) and observation group (69 cases). Patients in control group received non-pharmaceutical therapy of sleep hygiene education combined with ear point tapping. Patients in observation group received addition and subtraction treatment of Yangxin decoction by oral taking and foot massage. Course of treatment was 4 weeks for both groups. Before and after treatment, scores of Pittsburgh sleep quality index (PSQI), WHO quality of life scale (WHOQOL-BREF) and the clinician Global Impression (CGI) were graded. Result: Ridit analysis showed that the clinical effect in observation group was superior to that in control group (P<0.05). After treatment, the scores of PSQI factors (sleep quality, time of falling asleep, sleep time, sleep efficiency, sleep disorders, hypnotic and day function) and the total scores were decreased compared with the data before treatment in both groups (P<0.01). Scores of PSQI factors and the total scores in observation group were lower than those in control group (P<0.01). After treatment, scores of physiology, mentality, environmental and social relations were higher than the data before treatment (P<0.01). And scores of mentality and social relations in observation group were higher than those in control group (P<0.05). CGI scores were decreased in the second, third, and fourth weeks compared with the data at first week in both groups (P<0.05, P<0.01). There was no statistically significant difference between two groups. Conclusion: Addition and subtraction treatment of oral Yangxin decoction and foot massage intervention can improve the quality of sleep and enhance the life of patients in sub-health insomnia. The clinical effect is clear.关键词：sub-health insomnia;Yangxin decoction;pittsburgh sleep quality index;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Jiawei Qianghuo decoction combined with lonphoresis in the treatment of cervical spondylotic radiculopathy(CSA) with anemofrigid-damp arthralgia syndrome and its influence on hemorheology and interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in serum. Method: One hundred and thirty four patients with CSA were randomly divided into control group and treatment group according to number table, with 67 patients in each group. The control group was treated with Gentongping granule (8 g/1 time and 2 times/d) and massage manipulation with relax and traction on cervical vertebra (20 min/1 time and 1 time/d). Treatment group was given with additional Jiawei Qianghuo decoction (1 dose/d and oral, bid) and another Jiawei Qianghuo decoction was grinded into coarse powder, soaked in 75% wine for 1 month and heated for 10 min when it is used by iontophoresis (25 min/time, qd). The treatment course was 4 weeks for both groups. Clinical effect of both groups was compared. Subjective symptoms, adaptability, and clinical physical signs were assessed by the clinical assessment scale for cervical spondylosis (CASCS). Pain rating index (PRI) was adopted to evaluate the pain in both groups. IL-1β and TNF-α changes before and after treatment were analyzed in both groups. Result: The total effective rate of the treatment group and control group was 91.04% and 77.61% respectively, with significant difference between two groups (P<0.05). Each and total scores for CASCS in treatment group after treatment were higher than those in control group (P<0.05). Clinical symptoms and PRI scores in treatment group after treatment were significantly lower than those in control group(P<0.05). After treatment IL-1β and TNF-α in serum in treatment group were lower than those in control group (P<0.05). Conclusion: Oral Jiawei Qianghuo decoction combined with iontophoresis can improve clinical symptoms, physical signs and adaptability, relieve pain and reduce IL-1β and TNF-α in serum, with a good therapeutic effect.关键词：Jiawei Qianghuo decoction;iontophoresis;cervical spondylotic radiculopathy;interleukin-1β;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the curative effect of Juanbi Zhitong decoction by orally taking and fumigation on gonarthromeningitis according to Chinese and western medicine scheme. Method: One hundred and twenty-two patients were randomly divided into control group (62 cases) and observation group (60 cases) by random number table. Patients in control group received joint puncture and triamcinolone acetonide injection and hyaluronic acid sodium injection each for 20 mL after arthroedema was pumped, 1 time/7 days, for totally 2 times, and patients also orally took celecoxib capsules, 0.2 g/time, 1 time/day. Based on the treatment of control group, patients in observation group received additional Juanbi Zhitong decoction by orally taking and fumigation. Course of treatment was 4 weeks in both groups. Before and after treatment, scores of pain, swell and joint motion, as well as pain remission and disappearing time were recorded. Scores of Bristol knee-joint were graded, and levels of erythrocyte sedimentation rate (ESR) and C-reaction protein (CRP) were detected both before and after treatment. Result: Ridit analysis showed that the clinical effect in observation group was superior to that in control group, with significantly statistical difference (P<0.05). Scores of pain, swelling and joint motion in observation group were lower than those in control group (P<0.01). Average remission and disappearing time of pain in observation group was shorter than that in control group (P<0.05). Levels of ESR and CRP were lower than those in control group (P<0.05). After treatment, compared with the data before treatment, various dimensional scores of Bristol knee-joint and total scores increased obviously (P<0.01), and those scores in observation group were higher than the data in control group except the score of malformation (P<0.05, P<0.01). And excellent and good rate in observation group was 70% higher than 51.2% in control group (P<0.05). Conclusion: Juanbi Zhitong decoction by orally taking and fumigation combined with western medicine can relive symptoms of pain and swell, promote the recovery of joint function and improve quality in the treatment of gonarthromeningitis. It has a good effect in Chinese and western medicine scheme.关键词：gonarthromeningitis;Chinese and western medicine scheme;Juanbi Zhitong decoction
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Xuanbi decoction combined with Baihu Guizhi decoction by oral and washout methods in treating acute gouty arthritis (AGA) and their influence to blood uric acid. Method: One hundred and thirteen patients were randomly divided into control group (56 cases) and observation group (57 cases) at the proportion of 1:1.Patients in control group received colchicine tablets and ibuprofen tablets. Based on the treatment of control group, patients in observation group received additional Xuanbi decoction combined with Baihu Guizhi decoction by oral method. The treatment course was 14 days in both groups. Visual analogue scale(VAS) was used to evaluate arthralgia, tenderness, joints swollen and articular activity obstacle before and after treatment. Remission time was recorded. Blood uric acid (UA), erythrocyte sedimentation rate (ESR), and C-reactive protein (C-RP) were detected. Result: Ridit analysis showed that the comprehensive clinical effect in observation group was superior to that in control group (P<0.05). On the second, third, seventh and tenth day after treatment, VAS scores of pain in observation group were lower than those in control group (P<0.01). Remission time in observation group was shorter than that in control group (P<0.05). Scores of arthralgia, tenderness, joints swollen and articular activity obstacle in observation group were lower than those in control group (P<0.01). Besides, levels of ESR, UA and CRP in observation group were lower than those in observation group (P<0.01). Conclusion: Based on the routine western medicine treatment, Xuanbi decoction combined with Baihu Guizhi decoction by oral taking can relieve pain of AGA and inflammatory reactions, and it can control the seizure of AGA more quickly and effectively.关键词：acute gouty arthritis;Xuanbi decoction;Baihu Guizhi decoction;inflammatory factors
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发布时间：2024-01-04 - 摘要：Objective: To determine the possible mechanism in diabetic nephropathy with normoalbuminuria and the effect of Bailing capsule on intervention. Method: Two hundred diabetes patients without micro albuminuria were selected and randomly divided into 2 groups, NDN group (n=100) and control group (n=100). Two groups were studied to compare the clinical data and laboratory results. Logistic regression analysis was used for the impacting factors of renal impairment. The NDN group was further divided into 2 groups:routine group (n=50) was treated with insulin.The Bailing capsule group (n=50) was treated with Bailing capsule combined with insulin. After 8 weeks, the clinical data and laboratory results in two groups were compared before and after treatment. Result: Normoalbuminuria diabetic nephropathy patients showed that the ankle-brachial index (ABI), toe-brachial index (TBI) and glomerular filtration rate(eGFR) were lower than that of the control group [ABI (0.82±0.17) VS (1.07±0.51), TBI (0.64±0.16) VS (0.99±0.23), eGFR (49.5±6.5) VS(95.4±7.1) mL·min-1·(1.73 m2)-1] (P<0.05), serum 8-isoprostaglandin F2α(8-iso-PGF2α) and carotid intima-media thickness (CIMT) were higher than that of the control group [8-iso-PGF2α (18.72±3.2) VS (8.21±1.9) μg·L-1, CIMT (1.13±0.19) VS (0.71±0.17)mm] (P<0.05). GFR was significantly positively correlated with ABI and TBI in patients with normoalbuminuria diabetic nephropathy (r=0.432, 0.374, P<0.05). ABI, TBI and 8-iso-PGF2α were independent risky factors of renal impairment in patients with normoalbuminuria diabetic nephropathy (P<0.005, P<0.001, P<0.001). After treatment, the 8-iso-PGF2α in the Bailing capsule group was decreased more significantly than routine group [(9.34±2.8) VS (18.08±4.1) μg·L-1]. After treatment the ABI, TBI in the Bailing capsule group was increased more significantly than routine group [ABI (0.98±0.35) VS (0.81±0.35), TBI (0.92±0.23) VS (0.62±0.22)] (P<0.05). Conclusion: The incidence of normoalbuminuria diabetic nephropathy may be associated with atherosclerosis and oxidative stress. The intervention with Bailing capsule can delay the development of normoalbuminuria diabetic nephropathy.关键词：diabetes;diabetic nephropathy;oxidative stress;atherosclerosis;Bailing capsule
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effects of method for warming the kidney and strengthening the spleen, and dispersing blood stasis and promoting collaterals in the treatment of clinical-stage diabetic nephropathy (DN). Method: Fifty eligible DN patients were randomly divided into the treatment group (25 patients) and control group (25 patients). Besides the routine therapy including controlling diet, lowing blood glucose and orally taking fosinopril (10 mg daily), the treatment group was also given traditional Chinese medicine(TCM) for warming the kidney and strengthening the spleen, and dispersing blood stasis and promoting collaterals for 12 weeks. The changes in quantitation of 24-hour urinary protein (UP), serum creatinine (SCr), endogenous creatinine clearance rate (CCr), TCM syndrome score were observed in the patients. Result: The total effective rate of the treatment group was 80.0%, and that of control group was 56.0%. The effect of treatment group was better than the control group in lowing 24 h UP (P<0.05), reducing SCr (P<0.05), increasing CCr (P<0.05) and improving renal function (P<0.05). Conclusion: The method of warming the kidney and strengthening the spleen, and dispersing blood stasis and promoting collaterals can effectively abate and treat clinical-stage DN.关键词：warming the kidney and strengthening the spleen;dispersing blood stasis and promoting collaterals;diabetic nephropathy
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发布时间：2024-01-04 - 摘要：Objective: To probe into clinical application value and significance of Danhong injection for patients with acute ischemic stroke. Method: Totally 90 acute ischemic stroke patients treated in our hospital From June 2013 to June 2014 were selected. The patients were divided into study group and control group randomly. There were 45 cases in each group. The patients in control group were treated with conventional regimen, including lowering the intracranial pressure, anti-platelet aggregation and neuroprotective agents. The patients in study group were treated with Danhong injection in addition to the therapies in control group. The neurological recovery, changes of hemorheology and improvements in activities of daily living of the patients in two groups were compared and analyzed. Result: Compared with control group, the patients in study group showed significant increases in the ratio of neurological improvement at 46.67%(21/45) and total efficiency at 91.11%(41/45), the differences were significant statistically (P<0.05). Compared with control group, after treatment, the patients in study group showed significant decreases in the plasma viscosity at (1.39±0.21) mPa·s, whole blood viscosity at high shear value at (5.91±0.12) mPa·s, whole blood viscosity at low shear value at (7.18±1.34) mPa·s, hematocrit at (40.23±4.15)%, platelet adhesion rate at (26.73±5.24)%, the differences were significant statistically (P<0.05). Compared with control group, after treatment, the patients in study group showed significant increases in BI scores at (74.61±7.82) points and FMA scores at (54.88±5.36) scores, the differences were significant statistically (P<0.05). Conclusion: Danhong injection has extremely important clinical significance in recovering nerve function, improving hemorheology and enhancing daily living for patients with acute ischemic stroke. It was worth further applying and promoting.关键词：acute ischemic stroke;Danhong injection;nerve function;hemorheology
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发布时间：2024-01-04 - 摘要：Objective: To investigate the frequently-used traditional Chinese medicines(TCMs) and their properties and compatible regularity in the prescriptions for tonifying kidney, in order to provide reference for clinical drug screening and rational drug combination. Method: The prescriptions with effect of tonifying kidney were collected from the Chinese Prescription Database by using BICOMB. The TCMs were extracted and their names were standardized according to the textbook to form a word-word matrix. The frequency statistics and Graphical Clustering Toolkit gCLUTO were adopted for a clustering analysis and visibility processing of the TCMs. Result: The 358 TCMs were involved in the 251 prescriptions of tonifying kidney, and 25 were frequently used. Most of the prescriptions were derived from Dialectical Record and other five prescription books. The compatible regularity of the TCMs could be divided into three main and five minor categories. In each category, they show the effect of tonifying kidney by clearing damp, tonifying Yang, nourishing Yin, nourishing blood and supplementing Qi. Conclusion: Radix Rehmanniae Praeparata, Poria Cocos, Angelica Sinensis, Semen Corni and Rhizoma Dioscoreae are the frequently used TCMs for tonifying kidney. Most of them feature sweet taste, warm and mild properties and enter kidney, liver, spleen meridians, with nontoxic. The therapeutic effects are promoted through coordination and antagonism among TCMs.关键词：kidney deficiency;data mining;prescription;compatible regularity;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the methodological qualities of the systematic review/Meta-analysis (SR/MA) of Xingnaojing in treating stroke. Method: Chinese biomedical literatures and journals were searched through China National Knowledge Infrastructure, VIP, Wanfang and Cochrane databases. The systematic review/Meta-Analysis of Xingnaojing in treating stroke was included. The search time was ended in January 2015.The Oxman-Guyatt Overview Assessment Questionnaire (OQAQ) Scale was used to evaluate the methodological and reporting quality of the included SR/MA literatures. Result: A total of 13 SR/MA literatures were included. The included SR/MA had the following shortcomings:incomplete literature search, high selection bias, poor methodological quality of the original articles and low accuracy. The original articles in SR/MA had the following shortcomings:unclear randomizatioon methods, unclear concealment, no blinding, loss to follow-up or intent-to-treat (ITT), uncertain co-intervention or contamination. Conclusion: The methodological qualities of domestic SR/MA of Xingnaojing in treating stroke were poor. It was necessary to improve the methodological qualities of SR/MA and standardize the experiment design, especially to improve the literature search and reduce the selection bias. The criteria recommended by Cochrane shall be adopted for the quality evaluation of the included original literatures.关键词：Xingnaojing;stroke;systematic review;Meta-analysis;methodology;quality assessment
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发布时间：2024-01-04 - 摘要：Objective: To assess the effect and safety of edaravone combined with Danhong injection in treating acute cerebral infarction (ACI) by Meta-analysis. Method: Randomized control trials (RCTs) of edaravone combined with Danhong injection in treating ACI were retrieved from Cochrane Library, PubMed, MedLine, ScienceDirect, CNKI, Wanfang and VIP (from January 2000 to December 2014). The data was analyzed by using RevMan 5.1. Result: Twenty-five RCTs were included. The results showed that edaravone combined with Danhong injection had statistically significant differences compared with control group in the effective rate for ACI [OR=4.15, 95%CI (3.24, 5.21)], the neurological deficit scores [WMD=-3.75, 95%CI (-4.53, -2.96)] and the activities of daily living score [WMD=14.40, 95%CI (12.97, 15.83)]. Conclusion: Compared with edaravone treatment group, Danhong injection treatment group and routine treatment group, edaravone combined with Danhong injection has a better efficacy for ACI.关键词：edaravone;Danhong injection;acute cerebral infarction;systematic review
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发布时间：2024-01-04 - 摘要：Objective: This research uses traditional Chinese medicine(TCM) inheritance support system to analyze the composing principles of the prescriptions given by Professor Yin Changjian for treating cirrhosis. Method: We collected the prescription used for cirrhosis cases, then created the related database to store the related information. We further utilized the TCM inheritance support system to analyze the composing principles. By using principle analysis, we revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering to analyze composing principle. Result: We found 435 cases of prescriptions related to Cirrhosis. The frequency of each herb appeared in the database was calculated, and the most used 21 herbs including Amomi Fractus Rotundus, Galli Gigerii Endothelium Corneum, etc. The core combination is highly related to correlation between Amomi Fractus Rotundus and Amomi Fractus Rotundus or Polygoni Orientalis Fructus. Seven new prescriptions for different Cirrhosis stage are discovered. Conclusion: The approach used in this work, utilizing data mining methods(text mining and association relationship) based on TCM inheritance support system, clearly shows the composition principle of prescription for treating cirrhosis used by Professor Yin Changjian:the basical therapies are invigorating the spleen for eliminating dampness, invigorating the circulation of blood and removing dampness through diuresis, and resloving hard lump. Making a point of protecting stomach-Qi.Invigorating the spleen for resloving hard lump.Prescribing the herbs on the basis of diffent cirrhosis stage.关键词：traditional Chinese medicine inheritance support system;cirrhosis;Yin Changjian's composition principle
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发布时间：2024-01-04 - 摘要：Ixeris chinensis has efficacies of detoxification, blood circulation, anti-inflammatory and analgesia in the treatment of hyperactivity cough, appendicitis, bruises embolism.According to relevant domestic and foreign literatures, nearly 100 compounds were isolated from I. chinensis, with guaiac sesquiterpene lactone compounds as the dominance;and a variety of new guaiac sesquiterpene lactone compounds were separated from I. chinensis all the grass:8-epicrepioside, ixerochinolide, ixerochinoside, chinensiolides A, B, C, D, E, F, G, H, I, J, K, L, as well as flavonoids, triterpenes and sterols. Modern pharmacological studies have shown that I. chinensis has anti-inflammatory, hepatoprotective, antioxidant, anti-nicotine, anti-viral, anti-leukemia effects;in particularly, flavonoids have a strong pharmacological activity. Furthermore, I. chinensis, as a folk medicine, were mostly used in compounds and can be found in many patents for food and beverage, healthcare tea andcomposite pig feed. For the purpose of the rational use of medicinal resources, the relevant studies on the chemical composition of I. chinensis, pharmacological effects, development and application in recent years were summarized, in order to provide a reference for further studies and development of new drugs of I. chinensis.关键词：Ixeris chinensis;chemical constituents;pharmacological effects;development and application
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