最新刊期
      卷 21 ， 期 22 ， 2015
      • WANG Jie,LI Min,LIU Yong-mei,DUAN Lian
        Vol. 21, Issue 22, Pages: 1-5(2015) DOI: 10.13422/j.cnki.syfjx.2015220001
        摘要：miRNA, as a 19~23 nucleotide non-coding RNA, plays an important role in biological growth, differentiation, cell proliferation and apoptosis. miRNA played an important role in pathologic remodeling process of coronary artery disease(CAD) and injury in myocardial contractility. Further research found that some cardiac-specific miRNA exhibited certain a diagnostic value of diagnoses about CAD, which was closely associated with traditional markers. Traditional Chinese medicine (TCM) played an important role in treating coronary heart disease(CHD). According to studies on TCM and miRNA,miRNA is likely to be a new target for the theory development of TCM theory, and syndrome remarker and development R&D of TCM. This paper gives a brief introduction of miRNA, and focuses on the role of miRNA as a potential biomarker for treating(CHD) and its close relationship with TCM.  
        关键词：miRNA;CAD;TCM   
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        发布时间：2024-01-04
      • LIU Yong-mei,SUN Xiu-ling,LIU Rui-hua,WANG Jie
        Vol. 21, Issue 22, Pages: 6-10(2015) DOI: 10.13422/j.cnki.syfjx.2015220006
        摘要：Objective: To investigate the effect of panax notogin seng (PNS) on the expression of miRNA-466b and its apoptosis related proteins in myocardial cells of acute myocardial infarction (AMI) animal model, and to explore the post transcriptional molecular mechanism of the anti-apoptosis effect of PNS. Method: The 36 male wistar rats were randomly divided into 3 groups:sham operation group, model group, and PNS group. Coronary ligation of left anterior descending branch was done to establish acute myocardial infarction (AMI) models. PNS group rats were intramuscularly injected with PNS (1.429 mg·g-1) respectively on 4 h before AMI, 1 day and 2 days after AMI. TUNEL, RT-PCR and Western blot methods were used to observe the apoptosis of myocardial tissue in rats, and detect the expression of miRNA-466b, Caspase-3, Caspase-9 and Bcl2L13 genes and proteins. Result: Compared with model group, PNS treatment group could significantly reduce infarction area and apoptosis of myocardial cells (P<0.05), and there was no difference in miRNA-466b expression level between model group and sham operation group, while miRNA-466b in PNS group was significantly up-regulated, with significant difference with model group (P<0.01). Compared with sham operation group, the expression of Caspase-3 mRNA and protein in model group was significantly increased (P<0.05), and PNS can significantly down-regulate it (P<0.05, P<0.01), and changes in expression of Bcl2L13 and Caspase-9 mRNA and protein were not evident in model group, while PNS can significantly down-regulate the expression of these two indicators (P<0.05, P<0.01). Conclusion: PNS plays a key role in the protection of myocardial cells in rats with AMI, probably by up-regulating miRNA-466b, down-regulating Bcl2L13, Caspase-3 and Caspase-9 protein expression to inhibit cell apoptosis.  
        关键词：acute myocardial infarction;panax notogin seng;celluar apoptosis;microRNA   
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        发布时间：2024-01-04
      • DUAN Lian,LIU Yong-mei,LI Min,WANG Jie
        Vol. 21, Issue 22, Pages: 11-15(2015) DOI: 10.13422/j.cnki.syfjx.2015220011
        摘要：Objective: To observe the effect of tonifying kidney anti-hypertension decoction on SHR with abnormal blood lipid and blood glucose and adiponectin pathway. Method: Totally 40 5-week-old clean grade SHRs were randomly divided into the normal diet group and high-fat group. After the modeling at the 8th week, the high-fat group rats were divided into three groups:model group (blank SHR with abnormal glucose and lipid metabolism), captopril group and tonifying kidney group (tonifying kidney anti-hypertension decoction). After the treatment of 8 weeks, the rats were killed to test their blood pressure, glucose, blood lipid and insulin. Myocardial tissue sections were stained with HE to observe the pathological changes in heart. The content of ADP in serum was detected by Elisa assay. Result: Before the treatment with drugs, blood pressure of SHRs with a high sugar and fat diet had no statistical difference. Blood pressure of groups with a high sugar and fat diet was higher than those with normal diet (P<0.05). After the modeling at the 4th week, compared with the model group, captopril group showed a lower blood pressure significantly (P<0.05). After 8 weeks, compared with the model group, captopril group and tonifying kidney group showed statistically significant difference in the blood pressure (P<0.01). Compared with SHR group, model group, captopril group, tonifying kidney group showed decreased adiponectin, and model group had significant difference (P<0.05). In PPAR-mRNA of heart, the model group, captopril group and tonifying kidney group were higher than SHR group. Compared with the model group, captopril group and tonifying kidney group were lower. The expressions of AdipoR1 protein in model group, captopril group and tonifying kidney group were higher than that in SHR group. Compared with the model group, captopril group and tonifying kidney group were lower. AdipoR2 protein expression in model group, captopril group and tonifying kidney group were higher than that in SHR group. Compared with the model group and the captopril group, tonifying kidney group had a decreasing tendency. Conclusion: Tonifying kidney anti-hypertension decoction can regulate the abnormal blood lipid and blood glucose of SHRs and protect their hearts. Its mechanism may be correlated with the regulation of adiponectin pathway.  
        关键词：Tonifying kidney anti-hypertension decoction;adiponectin;blood pressure;lipid;blood glucose   
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        发布时间：2024-01-04
      • YUAN Rong,WANG Jie,GUO Li-li,LIN Fei,WEI Ben-jun,LI Jun-ping,CHEN Zhong
        Vol. 21, Issue 22, Pages: 16-20(2015) DOI: 10.13422/j.cnki.syfjx.2015220016
        摘要：Objective: To observe the effect of sleep deprivation(SD) stress on myocardial ischemia/reperfusion (I/R) injury, so as to discuss the sleep deprivation mechanism in aggravate myocardial I/R injury, while providing new ideas for studies on the effect of anxiety and insomnia on the development of coronary heart disease(CHD). Method: Experimental rats were randomly divided into sham operation, SD sham operation, I/R group and SD+I/R group, the modified multiple platform water environment method was used for seven-day sleep deprivation. Efforts were made to take the blood from rats eyes and then ligate left anterior descending coronary artery for 50 min, reperfused for 2, 24 h respectively to establish myocardial I/R model. The SD 7-day serum melatonin (MT), angiotensin (AngⅡ), endothelin (ET), nitric oxide (NO), interleukin 6(IL-6), tumor necrosis factor (TNF alpha) were measured; Ultrasound was used to observed cardiac ejection fraction (EF)at 2, 24 h after I/R, HE staining at 2 h after I/R was adopted to observe cardiac pathological structure and measure serum creatine kinase MB(CK-MB), lactate dehydrogenase (LDH) at 2 h after I/R. TTC Evans blue staining was used to determine the percent of myocardial infarction in ischemic area at 24 h after I/R. Result: After SD 7 days, the rats showed manic irritability, significant weight reduction, decline in serum MT levels, and obvious increases in AngⅡ, ET, IL-6, TNF-alpha. Serum CK-MB and LDH increased obviously at 2 h after I/R, ejection fraction (EF) were significantly decreased at 2, 24 h after I/R, and the percent of myocardial infarction in ischemic area significantly increased at 24 h after I/R.The pathological results showed the aggravated myocardial injury, with statistically significant differences from sham operation, SD sham operation and simple I/R group(P<0.05). Conclusion: The simple sleep deprivation stress can cause decrease in MT and increase in AngⅡ, ET, IL-6 and TNF-alpha, but without obvious effect on myocardial structure and function of rat. However, sleep deprivation could obviously aggravate myocardial ischemia/reperfusion injury. Its mechanism may be related to neuroendocrine disorders, decrease in melatonin, endothelial injury and inflammation, and shall be confirmed in further studies.  
        关键词：coronary heart disease;insomnia;sleep disorder;psycho-cardiology;neuroendocrine   
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        发布时间：2024-01-04
      • TAN Hui,WANG Jian,YIN Ting-ting,HU Jian-peng,HE Ling,XU Wei
        Vol. 21, Issue 22, Pages: 21-25(2015) DOI: 10.13422/j.cnki.syfjx.2015220021
        摘要：Objective: To discuss influence of Naoluo Xintong prescription on angiogenesis and rCBF in MCAO/R rats. Method: Wistar rats were randomly divided into sham group,model group and Naoluo Xintong group,model of MCAO/R rats was reproduced in accordance to suture method,and further were subdivided into three time points,such as 3,7,14 d.The content of thrombopoietin (TPO) and endothelin (ET) in brain tissue homogenate were determined by ELISA.Expressing of vascular endothelial growth factor (VEGF) protein in cerebrum was assayed by immunohistochemistry.The rCBF was dynamically measured by laser Doppler flowmeter. Result: Compared with sham group,the content of TPO and ET,VEGF protein significantly increased,rCBF reduced in Naoluo Xintong group.Compared with model group,the content of TPO and ET markedly reduced,the amount of VEGF protein and rCBF increased in Naoluo Xintong group.Compared within the same group,the content of TPO in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d,the content of ET in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d.The amount of VEGF protein in Naoluo Xintong group in 14 d increased by comparing with Naoluo Xintong group in 3 d.The rCBF in Naoluo Xintong group in 14 d markedly increased by comparing with Naoluo Xintong group in 3 d. Conclusion: Naoluo Xintong lyophilized powder can improve blood flow status,increase rCBF by regulating regulatory factor related to cruor,fibrinolysis,VEGF and promoting angiogenesis,which play an treatment role on ischemic cerebral vascular disease (ICVD).  
        关键词：Naoluo Xintong prescription;thrombopoietin;endothelin;vascular endothelial growth factor;regional cerebral blood flow (rCBF)   
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        发布时间：2024-01-04
      • PIAO Xin-xin,WANG Ning,WANG Guang-yun,YANG Fei-xue,XUAN Zi-hua,HU Rong-feng,DAI Min,WANG Jian
        Vol. 21, Issue 22, Pages: 26-30(2015) DOI: 10.13422/j.cnki.syfjx.2015220026
        摘要：Objective: The present study was designed to investigate the effects of Naoluo Xintong capsules on platelet aggregation and thrombus formation in rats with focal cerebral ischemia/reperfusion injury. Method: Male SD rats were randomly divided into sham operation group (n=10), modeling group (n=60). Middle cerebral artery occlusion/reperfusion (MCAO/R) models were induced by the intraluminal suture method. The successfully modeled rats were randomly divided into model group, Aspirin group (0.010 g·kg-1), Naoluo Xintong capsules high, middle and low dose groups (4.500, 2.250, 1.125 g·kg-1). Transmission electron microscope was used to observe the ultrastructure changes of hippocampal neurons. The levels of serum thromboxane B2(TXB2), 6-keto prostaglandin F1α(6-keto-PGF1α), platelet aggregation rate and in vivo thrombus formation time were detected. Result: Compared with the sham operation group, pathological changes of the neurons in model group were severely damaged, TXB2 level in serum was significantly increased, 6-keto-PGF1α level was significantly reduced, and platelet aggregation rates at 1,2,5 min and maximum aggregation rate in model group were significantly increased (P<0.05, P<0.01). Compared with the model group, pathological morphology of neurons in Naoluo Xintong capsules groups (1.125, 2.250,4.500 g·kg-1) was significantly improved. After Naoluo Xintong capsules treatment, the serum TXB2 level was significantly decreased. In contrast, the level of serum 6-keto-PGF1α was increased. The platelet aggregation rate in Naoluo Xintong capsules low, middle and high dose groups in 1 min and 2 min was significantly lower than that in the model group, and the platelet aggregation rate in Naoluo Xintong capsules low and high dose groups and maximum aggregation rate in low and the middle dose groups in 5 min were significantly decreased. The thrombus formation time in Naoluo Xintong capsules low and middle dose groups was markedly prolonged (P<0.05,P<0.01). Conclusion: Naoluo Xintong capsules can inhibit the platelet aggregation and thrombus formation in focal cerebral ischemia/reperfusion injury models, with certain protection effect for ischemic cerebral vascular diseases.  
        关键词：Naoluo Xintong capsules;focal cerebral ischemia/reperfusion;platelet aggregation;thrombus formation   
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        发布时间：2024-01-04
      • GUI Shuang-ying,LI Wan-rong,HUANG Yi-ming,WANG Jian
        Vol. 21, Issue 22, Pages: 31-33(2015) DOI: 10.13422/j.cnki.syfjx.2015220031
        摘要：Objective: To optimize formulation and preparation process of Peiyuan granules which will provide scientific references for clinical development of Peiyuan granules. Method: Single factor tests were performed to choose formulation and preparation process,with formability,hygroscopicity and fluidity as indexes.Moisture content,fluidity,bulk density and critical relative humidity of Peiyuan granules were inspected. Result: Optimum process conditions of Peiyuan granules were as follows:dry extract powder-dextrin-soluble starch (5:3:3), wetting agent of 90% ethanol,wet granulation dried at 60℃ for 1 h.Yield,moisture content,angle of repose,bulk density and critical relative humidity were 95.78%,5.08%,30.73°,0.526 g·mL-1,70%. Conclusion: This formation process is reasonable and feasible.Moisture content,fluidity and bulk density of Peiyuan granules are up to requirements of the 2010 edition of Chinese Pharmacopoeia.  
        关键词：Peiyuan granules;formulation;preparation process;hygroscopicity;fluidity;critical relative humidity   
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        发布时间：2024-01-04
      • WANG Chao,LIU Chun-fang,LIN Na
        Vol. 21, Issue 22, Pages: 34-39(2015) DOI: 10.13422/j.cnki.syfjx.2015220034
        摘要：In conjunction with the title search and MeSH retrieval from CNKI, Pubmed, Web of science and other databases, we screened literature mentioned mechanisms of neuropathic pain(NP) for Chinese medicine intervention in the past 20 years. By Summarizing and analysising the literature, 70 articles were selected, and the main aspects that mentioned for NP treatment with Chinese medicine are as following:descending inhibition/facilitation systems, ion channels, membrane receptors, neuroinflammation, glial cell activation, signaling pathways, antiapoptotic, antioxidative stress, enzyme activity inhibition, et al. Collectively, Chinese medicine treatment for NP hyperalgesia are effective, with characteristics of multitargets intervention, not only by inhibiting nerve impulses, Chinese medicine can also improve NP neurogenic inflammation, Chinese medicine intervention for NP neuroinflammation gradually attracted scholars' attention, yet, in-deep studies are further needed.  
        关键词：neuropathic pain;NP descending inhibition/facilitation systems;ion channels;membrane receptors;mechanisms   
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        发布时间：2024-01-04
      • CHEN Ting,LI Xin,QU Ya-kun,XU Ying,LIN Na
        Vol. 21, Issue 22, Pages: 40-44(2015) DOI: 10.13422/j.cnki.syfjx.2015220040
        摘要：Objective: To observe the estrogen-like effect of the combined administration of Veratri Nigri Radix and Salviae Miltiorrhizae Radix et Rhizoma(SMRR) using immature mice model, so as to provide a scientific basis for eighteen incompatible medicaments Veratri Nigri Radix and SMRR according to theories of traditional Chinese medicine. Method: Totally 10021-day-old healthy Kunming female mice were randomly divided into 10 groups:normal, estradiol valerate (E2, 0.1 g·kg-1), E2 plus estrogen receptor antagonist (ICI, 0.005 g·kg-1), E2 plus Veratri Nigri Radix (VN, 0.045 g·kg-1), SMRR (SM, 1.6, 3.2 g·kg-1), SM plus ICI, SM plus VN for 7 days respectively, with 10 mice in each group. The estrogen-like effect was detected by observing uterus index, E2 in serum, LH and FSH levels and histomorphology changes in uterus and vagina. Result: Compared with normal group, SM groups showed significant increase in the uterus index (P<0.01, P<0.05) and decrease in the level of E2 (P<0.01), the level of LH (P<0.05) and FSH (P<0.01, P<0.05), and the growth and development of uterus and vagina in immature mice, with a similar effect to E2 (P<0.01). In SM plus VN group, uterus index decreased(P<0.05, P<0.01), level of E2 notably decreased (P<0.01, P<0.05), level of LH (P<0.01) and FSH (P<0.05, P<0.01) increased, and the growth and development of uterus and vagina decreased, with a similar trend to the SM plus ICI group. Meanwhile, in E2 plus VN group, uterus index decreased(P<0.01), level of E2 notably decreased (P<0.01), level of LH and FSH increased (P<0.01), and the growth and development of uterus and vagina decreased, with a similar effect to SM plus ICI group. Conclusion: These findings rprove that SMRR exerts estrogenic effect, VN has the anti-estrogen effect and can antagonize the estrogenic efficacy of SMRR.  
        关键词：Salviae Miltiorrhizae Radix et Rhizoma;Veratri Nigri Radix;antagonize;estrogen;uterus index   
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        发布时间：2024-01-04
      • LI Xin,CHEN Ting,QU Ya-kun,XU Ying,LIN Na
        Vol. 21, Issue 22, Pages: 45-49(2015) DOI: 10.13422/j.cnki.syfjx.2015220045
        摘要：Objective: To investigate the estrogenic effect of the incompatibility of Veratri Nigri Radix(VN) and Paeoniae Radix Alba(PRA) on immature mouse, so as to provide a scientific basis for eighteen incompatible medicaments VN and PRA. Method: One hundred female Kunming mice were randomly divided into 10 groups:the normal group, the PRA groups (4, 2 g·kg-1), estrogen receptor agonist group (E2, 0.1 g·kg-1), PRA+VN groups (PRA 4, 2 g·kg-1+VN 0.045 g·kg-1), E2 and VN group(E2 0.1 g·kg-1+VN 0.045 g·kg-1), PRA+estrogen receptor antagonist(ICI 182780) groups (PRA 4, 2 g·kg-1+ICI 0.005 g·kg-1), E2 group (0.1 g·kg-1) and ICI group (E2 0.1 g·kg-1+ICI 0.005 g·kg-1). Six days later, the immature mouse uterus coefficient was calculated. E2, LH and FSH were detected. Uterus and vagina pathological changs were observed by HE staining. Result: Compared with normal group, PRA and E2 groups showed significantly increase in the uterus index (P<0.01) and the level of E2 (P<0.05), decrease in the level of LH(P<0.01) and FSH(P<0.01), and the growth and development of uterus and vagina of the immature mouse, with a slightly weaker effect to E2. in the PRA plus VN group, uterus index decreased (P<0.01), level of E2 notably decreased(P<0.01), level of LH (P<0.01) and FSH (P<0.01)increased, and the growth and development of uterus and vagina decreased, with the same effect to PRA plus ICI. Conclusion: These findings prove that PRA exerts estrogenic effect, VN has the anti-estrogen effect and can antagonize the estrogenic efficacy of PRA.  
        关键词：Veratri Nigri Radix;Paeoniae Radix Alba;immature mouse;impair;estrogen   
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        发布时间：2024-01-04
      • SUN Dan-ni,WANG Qian-qian,WANG Chao,LIU Chun-fang,LIN Na
        Vol. 21, Issue 22, Pages: 50-54(2015) DOI: 10.13422/j.cnki.syfjx.2015220050
        摘要：Objective: To study the effect of the combined administration of Aconiti Radix Cocta and Ampelopsis Radix at different dose or ratio on formalin-induced licking time in mice. Method: The uniform design method was used based on two factors and seven levels to investigate licking time of formalin-intraplantar model at two phases, meanwhile, a demonstration test was conducted by choosing significant ratio and dose. Result: The combined administration of Aconiti Radix Cocta and Ampelopsis Radix availably reduced the licking time of two phases. Neither Radix ampelopsis nor Aconiti Radix Cocta used alone had the analgesic effect by regression analysis. There was the synergistic effect between Ampelopsis Radix and Aconiti Radix Cocta, and Ampelopsis Radix could increase the analgesic effect of Aconiti Radix Cocta in mice, with the peak at the rate of 1:1. Conclusion: The combined administration of Ampelopsis Radix and Aconiti Radix Cocta could increase the analgesic effect of Aconiti Radix Cocta, with the peak at the rate of 1:1. This study provides the laboratory basis for further researches on the combined effect between Ampelopsis Radix and Aconiti Radix Cocta.  
        关键词：eighteen incompatible pairs;uniform design;Aconiti Radix Cocta;Ampelopsis Radix;analgesic effect   
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        发布时间：2024-01-04
      • SUN Dan-ni,WANG Qian-qian,WANG Chao,LIU Chun-fang,LIU Wei-wei,LIN Na
        Vol. 21, Issue 22, Pages: 55-59(2015) DOI: 10.13422/j.cnki.syfjx.2015220055
        摘要：Objective: To study the influence of the combined administration of Trichosanthis Fructus and Aconiti Radix Cocta at different dose or ratio on thier expectorant and antitussive effects. Method: The uniform design method was used based on two factors and seven levels to investigate phenolsulfonphthalein excretion and number of cough in mice and the influence of the combined administration of Trichosanthis Fructus and Aconiti Radix Cocta at different dose or ratio on theirexpectorant and antitussive effects. A demonstration test was conducted by choosing significant ratio and dose. Result: Combined administration of Trichosanthis Fructus and Aconiti Radix Cocta at most ratios can increase the phenolsulfonphthalein excretion in mice. According to the regression analysis, Trichosanthis Fructus had an expectorant effect in mice. And the expectorant effect of the combined administrationof Trichosanthis Fructus and Aconiti Radix Cocta was not lower than that of the single administration of Trichosanthis Fructus in mice. At the rate of 1:2 between Trichosanthis Fructus and Aconiti Radix Cocta, the expectorant effect of the combined administrationwas almost the worst. Both Trichosanthis Fructus and Aconiti Radix Cocta at all ratios decreased the times of cough. According to the regression analysis, the combined administration of Trichosanthis Fructus and Aconiti Radix Cocta had good antitussive effect, but they had not an interactive effect with each other. Conclusion: The combined administration of Trichosanthis Fructus and Aconiti Radix Cocta could decrease the expectorant effect of Trichosanthis Fructus, but had no effect on the antitussive effect of Trichosanthis Fructus.  
        关键词：eighteen incompatible pairs;uniform design;Trichosanthis Fructus;Aconiti Radix Cocta;expectorant effect;antitussive effect   
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        发布时间：2024-01-04
      • HE Jing-xia,YANG Bin,KE Hua-xiang,YIN Rong-li,ZHANG Ji-dan,LI Hua
        Vol. 21, Issue 22, Pages: 60-63(2015) DOI: 10.13422/j.cnki.syfjx.2015220060
        摘要：Objective: To establish quantitative models of paeoniflorin and water in samples of Paeoniae Radix Alba using near infrared (NIR) spectroscopy. Method: 108 batches of Paeoniae Radix Alba samples were collected from different regions of China. Near infrared spectrum correction models for determination of paeoniflorin and water in samples were developed with the application of partial least squares regression. The reference analyses were performed with oven-drying method and ultra performance liquid chromatography method respectively for determination of water and paeoniflorin. Correlation coefficient and predicted root mean square error were also used for evaluating the estimated performance of the models. Result: For paeoniflorin and water, the correlation coefficients for calibration set were 0.9676 and 0.8731, respectively;root-mean-square errors for calibration set were 0.406 and 0.264, respectively;root-mean-square errors for prediction set of the two analytes reached 0.599 and 0.252, respectively. Conclusion: This method is rapid, simple, reliable and has no pollution, which can be used for rapid and accurate determination of paeoniflorin and water in Paeoniae Radix Alba.  
        关键词：near infrared spectroscopy;Paeoniae Radix Alba;paeoniflorin;water;content determination   
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        发布时间：2024-01-04
      • HE Jing-xia,YANG Bin,KE Hua-xiang,YIN Rong-li,ZHANG Ji-dan,LI Hua
        Vol. 21, Issue 22, Pages: 64-67(2015) DOI: 10.13422/j.cnki.syfjx.2015220064
        摘要：Objective: To rapidly determine the contents of paeonol and water in Moutan Cortex using near infrared (NIR) spectroscopy combined with partial least squares (PLS). Method: 108 batches of Moutan Cortex samples were collected from different regions of China. Near infrared spectrum correction models were established using PLS method for determination of paeonol and water content. The reference analyses were performed with oven-drying method and ultra performance liquid chromatography method respectively for water and paeonol content. Correlation coefficient and predicted root mean square error were also used for evaluating the estimated performance of the models. Result: The correlation coefficients for calibration set were 0.9593 and 0.8738 respectively for paeonol and water content;root mean square error of calibration was 0.114 and 0.167, respectively;root mean square error of prediction set was 0.136 and 0.157, respectively. Conclusion: This method is rapid, simple, and has no pollution, which can be used for rapid determination of paeonol and water in Moutan Cortex.  
        关键词：near infrared spectroscopy;Moutan Cortex;paeonol;water;partial least squares   
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        发布时间：2024-01-04
      • ZHANG Ji-dan,LI Hua,KE Hua-xiang,HE Jing-xia,SHAO Ai-juan,YANG Bin
        Vol. 21, Issue 22, Pages: 68-71(2015) DOI: 10.13422/j.cnki.syfjx.2015220068
        摘要：Objective: To develop rapid determination models of water and schisandrin in Schisandra Chinensis Fruit using near infrared (NIR) spectroscopy. Method: 93 batches of Schisandra Chinensis Fruit samples were collected from different regions of China. Near infrared spectrum correction models for determinations of schisandrin and water contents in samples were developed with the application of partial least squares regression. The reference analyses were performed with oven-drying method and ultra performance liquid chromatography method respectively for water and schisandrin. Correlation coefficient and predicted root mean square error were also used for evaluating the estimated performance of models. Result: The correlation coefficients for calibration models were 0.8767 and 0.8890 respectively for water and schisandrin;and root mean square error of prediction set was 0.121and 0.0219 respectively. Conclusion: This established near infrared spectrum method is rapid, reliable and has no pollution, which can be used for the quality evaluation of Schisandra Chinensis Fruit on site.  
        关键词：near infrared diffuse reflectance spectroscopy;Schisandra Chinensis Fruit;schisandrin;water;content determination   
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        发布时间：2024-01-04
      • LI Hua,KE Hua-xiang,HE Jing-xia,ZHANG Ji-dan,HE Yu-xin,LI Ling,YANG Bin
        Vol. 21, Issue 22, Pages: 72-76(2015) DOI: 10.13422/j.cnki.syfjx.2015220072
        摘要：Objective: To develop quantitative methods for rapid and nondestructive determination of phenolic compounds and water in Magnolae Officinalis Cortex using near-infrared (NIR) diffuse reflectance spectroscopy. Method: 99 batches of Magnolae Officinalis Cortex samples were collected from different regions of China.Partial least squares (PLS) method was used to establish NIR quantitative models for determinations of water and phenolic compounds in samples. The reference analyses were performed with oven-drying method and ultra performance liquid chromatography method respectively for determination of water and phenolic compounds. Correlation coefficient and predicted root mean square error were also used for evaluating the estimated performance of the models. Result: For water, magnolol, honokiol, and magnolol+honokiol, root mean square errors of calibration set were 0.156, 0.120, 0.133 and 0.236, respectively;the correlation coefficients were 0.8728, 0.9899, 0.9769 and 0.9812, respectively;and principal components were 4, 6, 9 and 5, respectively. Predicted root mean square errors of the four analytes reached 0.161, 0.154, 0.179 and 0.248, respectively. Conclusion: The developed analytical method based upon NIR spectroscopy was proved to be rapid, accurate, and non-destructive, which can be used for rapid quality evaluation of Magnolae Officinalis Cortex.  
        关键词：near infrared spectroscopy;Magnolae Officinalis Cortex;water;phenolic compounds;quantitative model   
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        发布时间：2024-01-04
      • CHEN Ying,LIU Hui,LI Pu-ling,CHEN Jian-hong,LI Ling yun,LIU Meng-jiao,MA Yin-lian,ZHANG Cun,TU Ya
        Vol. 21, Issue 22, Pages: 77-81(2015) DOI: 10.13422/j.cnki.syfjx.2015220077
        摘要：Objective: To identify three pieces of Scutellariae Radix (crude,processed by wine and fried into carbon) through infrared spectroscopy technique with baicalin as a standard. Method: 1D and 2D infrared spectroscopy were adopted to clarify potential change variation of three pieces of Scutellariae Radix by comparing their infrared spectra characteristic. Result: Spectra of three products were similar in 1D infrared spectroscopy.In the second derivative spectra,absorption peaks at 1357 cm-1 and 1296 cm-1 were moved to higher and lower wavenumbers after charred,respectively;absorption peak at 1413 (1408) cm-1 in crude and processed by wine were disappeared after charred.There were significant differences in 2D infrared spectroscopy.During 800-1300 cm-1,three characteristic peaks were shown in crude products with the strongest peak was at 1078 cm-1;processed products by wine had four characteristic peaks with the strongest peak was at 1066 cm-1;the strongest peak was at 1129 cm-1 in fried into carbon products which had three characteristic peaks.Four characteristic peaks were shown between 1300-1800 cm-1,the strongest peaks of crude,processed by wine and fried into carbon products were at 1576,1620,1558 cm-1,respectively. Conclusion: Infrared spectroscopy technique can provide a great deal of information,which can provide a scientific basis for further explanation of change variation of processed products of Scutellariae Radix.  
        关键词：infrared spectroscopy;Scutellariae Radix;pieces;baicalin;processing of traditional Chinese medicine   
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        发布时间：2024-01-04
      • LI Pu-ling,CHEN Jian-hong,LIU Hui,MA Yin-lian,CHEN Ying,LIU Meng-jiao,LI Ling-yun,ZHANG Cun,TU Ya
        Vol. 21, Issue 22, Pages: 82-85(2015) DOI: 10.13422/j.cnki.syfjx.2015220082
        摘要：Objective: To establish a method for identifying different processed products of Gardeniae Fructus by comparing their infrared spectroscopy. Method: Infrared spectroscopy and two-dimensional spectroscopies as well as reference of geniposide were employed to distinguish three different processed products of Gardeniae Fructus so as to find their change variation. Result: One-dimensional spectrum pattern of three different processed products of Gardeniae Fructus was similar.In the second derivative spectra,characteristic peaks of raw,stir-baked and fried into carbon of Gardeniae Fructus were 1228,1235,1241 cm-1,respectively.In two-dimensional correlation infrared spectrum during 1300-1800 cm-1,the strongest automatic peaks of raw,stir-baked and fried into carbon of Gardeniae Fructus were 1636,1640,1740 cm-1,respectively. Conclusion: Infrared spectroscopy and two-dimensional spectroscopies can be used to identify different processed products of Gardeniae Fructus.  
        关键词：Gardeniae Fructus;pieces;stir-frying;geniposide;infrared spectroscopy   
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        发布时间：2024-01-04
      • WANG Jing-jing,LIANG Juan-juan,CHEN Liang-mian,GAO Hui-min,WANG Zhi-min,XIAO Wei,WANG Zhen-zhong
        Vol. 21, Issue 22, Pages: 86-93(2015) DOI: 10.13422/j.cnki.syfjx.2015220086
        摘要：Objective: To develop quantitative analysis of multi-components with single marker (QAMS) for determination of butylphthalide, senkyunolide A, and Z-ligustilide in Tianshu capsule. Method: An HPLC method was developed for simultaneous determination of butylphthalide, senkyunolide A and Z-ligustilide, and the chromatographic peaks were identified based on the UV spectra and accurate molecular weights obtained by UPLC-Q-TOF/MS. With butylphathlide as the internal substance, relative correction factors (RCFs) of senkyunolide A and Z-ligustilide to butylphathlide were calculated at single wavelength and different wavelengths respectively through slope analysis method. Relative retention value method was used for positioning of chromatographic peaks;external standard method (ESM) was used to verify the result accuracy. Result: QAMS method was established for the determination of butylphthalide, senkyunolide A and Z-ligustilide in Tianshu capsule and RCFs of senkyunolide A and Z-ligustilide to butylphathlide were 0.272 and 1.056, respectively. The contents of these three marker compounds were 0.008%-0.036%, 0.023%-0.125% and 0.028%-0.084% in 18 batches of Tianshu capsule samples. No significant differences on the quantitative results were found between QAMS and ESM. Conclusion: The established QAMS method with butylphathlide as the internal substance could be used for the determination of butylphthalide, senkyunolide A and Z-ligustilide in finished products and manufacturing process of Tianshu capsule.  
        关键词：quantitative analysis of multi-components with single marker;relative correction factor;Tianshu capsule;HPLC;UPLC-QTOF/MS   
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        发布时间：2024-01-04
      • MO Shu,ZHANG Hong-min,HAN Fu-man,WANG Li-xin,WANG Jin-yu,TONG Yan,FENG Wei-hong,LIU Jian-xun
        Vol. 21, Issue 22, Pages: 94-98(2015) DOI: 10.13422/j.cnki.syfjx.2015220094
        摘要：Objective: To establish a new method for quantitative analysis on multi-components by single marker (QAMS) and validate its feasibility and technical adaptability in analysis on Quyu Huatan Tongmai granules for simultaneous determination of 12 main constituents. Method: Quyu Huatan Tongmai granules was chosen as research object,taking berberine hydrochloride as index component,relative correction factors of sodium danshensu,salvianolic acid B,cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA,ferulicacid,epiberberine,coptisine,palmatine hydrochloride,chlorogenic acid and lovastatin were established by HPLC and calculated to achieve QAMS.Contents of 12 components in samples were determined with the external standard method,difference between two methods was compared and to validate correctness and adaptability of QAMS. Result: No significant differences were observed between quantitative results of these two methods with RSD<5%. Conclusion: This established QAMS method can be used for quantitative determination of Quyu Huatan Tongmai granules.It can provide references for quality control of Chinese patent medicine.  
        关键词：quantitative analysis of multi components by single marker;relative correction factor;Quyu Huatan Tongmai granules;ferulic acid;quality evaluation;berberine hydrochloride   
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        发布时间：2024-01-04
      • QIAO Yuan-yuan,YU Ting-ting,ZHENG Cai-xia,WEI Yi,TAN Rui
        Vol. 21, Issue 22, Pages: 99-102(2015) DOI: 10.13422/j.cnki.syfjx.2015220099
        摘要：Objective: To establish a quantitative assay of multi-components by single marker (QAMS) for determination of four bioactive components in Lancea tibetica, and examine the accuracy and feasibility of the relative correction factors (RCFs) between different types of compounds. Method: L. tibetica was chosen as the research object, and RCFs were determined between acteoside and catalpol, phillyrin and phillygenol. The contents of the four constituents were also calculated according to the RCFs to carry out QAMS. Difference of QAMS and external standard method was investigated to validate the accuracy and feasibility of the QAMS method. Result: RCFs between acteoside and catalpol, phillyrin and phillygenol were 7.0449, 2.1350 and 0.9647 respectively within a certain linear range;repeatability was good in different experimental conditions (RSDs were 0.3%, 1.2% and 1.2% respectively);there was no significant differences between the quantitative results of QAMS method and external standard method for 4 constituents. Conclusion: It is accurate and feasible to evaluate 4 constituents of L. tibetica by QAMS. Therefore, this method is suitable to determine different types of components, and can provide a new reference for the quality assessment of Tibetan herb-medicine.  
        关键词：quantitative analysis of multi components by single marker;Tibetan Herb medicine;Lancea tibetica;relative correction factors   
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        发布时间：2024-01-04
      • FU Ling-yun,HUANG Hai-feng,XU Yi-ni,ZHANG Yan-yan,LUO Hong,TAO Ling,CHEN Yan,SHEN Xiang-chun
        Vol. 21, Issue 22, Pages: 103-107(2015) DOI: 10.13422/j.cnki.syfjx.2015220103
        摘要：Objective: To investigate inhibitory effect of oxymatrine (OMT) on proliferation of rat cardiac fibroblasts (CFs) induced by aldosterone (ALD),and then explore its mechanism. Method: Primary CFs was digested by trypsin with neonatl rats,and then was purified by differential adhension.Expeiremts were designed 4 groups as following:blank group,model group (1×10-7 mol·L-1 of aldosterone),OMT low-dose group of 3.78×10-4 mol·L-1,OMT high-dose group of 7.57×10-4 mol·L-1.Vimentin of CFs was identified by immunocytochemistry.Inhibitory effect of OMT on CFs proliferation was detected by MTT assay.Real-time PCR was employed to determine mRNA level of p38 mitogen-activated protein kinase (p38MAPK).Expression of phosphorylation-p38MAPK (p-p38MAPK) and p38MAPK was analyzed by Western blot. Result: Optimal proliferation of CFs induced by ALD with 1×10-7 mol·L-1 at 24 h.OMT with 7.57×10-4 mol·L-1 could significantly inhibit proliferation of CFs.OMT could not affect expression of p38MAPK mRNA,but it attenuated phosphorylation of p38MAPK induced by ALD. Conclusion: OMT inhibits proliferation of CFs induced by ALD,and its mechanism may be involved in inhibiting phosphorylation of p38MAPK.  
        关键词：oxymatrine;aldosterone;cardiac fibroblasts;mitogen-activated protein kinase   
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        发布时间：2024-01-04
      • LIU Xiao-hong,YANG Li-qiang,XU Yi-ni,ZHANG Yan-yan,PAN Di,ZHANG Bei,CHEN Yan,TAO Ling,SHEN Xiang-chun
        Vol. 21, Issue 22, Pages: 108-111(2015) DOI: 10.13422/j.cnki.syfjx.2015220108
        摘要：Objective: To observe effect of oxymatrine (OMT) on expression of extracellular signal-regulated kinase 1/2 (ERK1/2) in cardiomyocytes induced by aldosterone (ALD). Method: Pancreatic enzyme digestion method was used to extract caridac myocytes and purity of cultured caridac myocytes was evaluated by immunocytochemistry.Cardiac myocytes were pre-incubated with oxymatrine of high-does and low-does for 1 h,and then exposed to ALD for 24 h.Cell injury was evaluated by MTT assay and LDH leakage.Protein expression of ERK1/2 and phosphorylation-ERK1/2 (p-ERK1/2) was detected by Western blot and ERK mRNA was detected by real time polymerase chain reaction (PCR). Result: After co-incubation with OMT could enhance MTT volume and decrease LDH activity in medium induced by ALD,there was significant difference by comparing with model group.Meanwhile,expression of p-ERK1/2 was significantly down-regulated after co-incubation with OMT. Conclusion: OMT has an protective effect on caridac myocytes induced by ALD,its mechanism may be related to inhibition of p-ERK1/2.  
        关键词：oxymatrine;cardiac myocytes;extracellular signal-regulated kinase;aldosterone;phosphorylation   
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        发布时间：2024-01-04
      • WEN Bo,LINGHU Ke-gang,XU Yi-ni,ZHANG Yan-yan,LUO Hong,TAO Ling,CHEN Yan,SHEN Xiang-chun
        Vol. 21, Issue 22, Pages: 112-115(2015) DOI: 10.13422/j.cnki.syfjx.2015220112
        摘要：Objective: To investigate protective effect of essential oil from Fructus Alpinia zerumbet (EOFAZ) on human aortic endothelial cells (HAECs) injury induced by oxidized low density lipoprotein (ox-LDL),and explore its possible mechanism. Method: Experiment was randomly divided into 4 groups as following:blank group (serum free ECM),model group (200 mg·L-1 of ox-LDL),EOFAZ with high dose group (200 mg·L-1 of ox-LDL+100 μg·L-1 of EOFAZ) and low dose group (200 mg·L-1 of ox-LDL+10 μg·L-1 of EOFAZ).Pretreated with EOFAZ for 1 h and continue added ox-LDL for 24 hours.Then 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method was employed to analyze cell survival rate and HE staining was used to observe morphology.Leakage of lactate dehydrogenase (LDH) was detected by enzyme-linked immunosorbent assay (ELISA).Expression of c-Jun N-terminal kinase 1/2/3 (JNK1/2/3) and phosphorylation-JNK1/2/3 (p-JNK1/2/3) protein was detected by Western blot analysis and JNK1/2/3 mRNA level was detected by quantitative real time polymerase chain reaction (qRT-PCR). Result: EDFAZ could significantly enhance survival rate of HAECs,ameliorate pathological injury of cells,decrease LDH leakage content and inhibit phosphorylation of JNK1/2/3,but it had no effect on expression level of JNK1/2/3 protein and mRNA. Conclusion: EDFAZ can ameliorate HAECs injury status induced by ox-LDL,and its mechanism may be related to inhibition of phosphorylation of JNK1/2/3.  
        关键词：Alpinia zerumbet;essential oil;oxidized low density lipoprotein;human aortic endothelial cells;c Jun N terminal kinase   
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        发布时间：2024-01-04
      • WANG Ya-jie,YANG Qing,LI Yu-jie,WANG Jun-li,CAI Wei-yan,WENG Xiao-gang,LI Qi,CHEN Ying,KAN Xiao-xi,GUO Yan,CHEN Xi,HUANG He-fei,GUAN Jing,ZHU Xiao-xin
        Vol. 21, Issue 22, Pages: 116-121(2015) DOI: 10.13422/j.cnki.syfjx.2015220116
        摘要：Objective: To study the antipyretic, anti-inflammatory and analgesic effect of Xiaoer Qingwen Jiere granules. Method: Wistar rats and Japanese white rabbits were selected. Antipyretic effect was investigated by subcutaneous injection of yeast induced fever in rats models and endotoxin induced fever in rabbits models. KM mice were selected, and acetic acid writhing and hot plate stimulation method was used to observe the analgesic effect. ear swelling, capillary permeability and hind paw inflammation swelling in ICR mice and SD rats were observed for investigating the anti-inflammatory effect. Result: Administration of Xiaoer Qingwen Jiere granules at 3.92 g·kg-1 and 15.68 g·kg-1 for 3 h could significantly reduce the rabbit anal temperature difference (P<0.05, P<0.01), and administration of 3.78-30.24 g·kg-1 for 4 h could significantly reduce rat rectal temperature (P<0.05, P<0.01), administration of 43.68 g·kg-1 could significantly reduce the writhing times of mice (P<0.05), and 10.92, 21.84 g·kg-1 for 2 h could significantly improve pain threshold of mice (P<0.05). Administration of 5.46-43.68 g·kg-1 continuously for 3 days could significantly reduce xylene induced right ear swelling in mice (P<0.01), 7.56 g·kg-1 could significantly reduce foot swelling degree (P<0.05),15.12 g·kg-1 for 0.5, 2, 4 h could significantly reduce foot swelling degree (P<0.05, P<0.01),30.24 g·kg-1 for 0.5-4 h could significantly reduce foot swelling degree (P<0.05, P<0.01).At 43.68 g·kg-1, absorbance value of peritoneal washing liquid was significantly lower than that in model group (P<0.05). Conclusion: Xiaoer Qingwen Jiere granule has antipyretic, anti-inflammatory and antipyretic analgesic effects.  
        关键词：Xiaoer Qingwen Jiere granule;antipyretic;anti-inflammatory;analgesic   
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        发布时间：2024-01-04
      • CHEN Xi,YANG Qing,LI Qi,CHEN Ying,KAN Xiao-xi,HUANG He-fei,LI Yu-jie,WANG Ya-jie,CAI Wei-yan,WENG Xiao-gang,LIU Si-si,GUO Shan-shan,ZHU Xiao-xin,GUAN Jing
        Vol. 21, Issue 22, Pages: 122-127(2015) DOI: 10.13422/j.cnki.syfjx.2015220122
        摘要：Objective: To study the effect of Xiaoer Qingwen Jiere Granule in regulating immune function in mice, and its antibacterial efficacy in vitro or in vivo. Method: Totally 70 Balb/C mice were divided into following groups:normal, model, Yupingfeng granule, thymosin, and low, medium, high doses of Xiaoer Qingwen Jiere granules (10.92, 21.84, 43.68 g crude drug/kg) according to body weight, with 10 in each group. Immune deficiency model was constructed through intraperitoneal injection with cyclophosphamide in all groups except for the normal group, in order to observe the influence of Xiaoer Qingwen Jiere granule on the T or B lymphocyte proliferation, carbon clearance ability and serum and hemolysin content. ICR mice were randomly divided into seven groups by weight:model group, amoxicillin group (370 mg·kg-1), Xiaoer Ganmaoning mixture group (8.25 mL·kg-1) and low, medium, high-dose Xiaoer Qingwen Jiere granules groups, with 20 to 22 mice in each group. Bacterial infection model was constructed through intraperitoneal injection with Staphylococcus aureus to observe the animal mortality rate, drug protection and the average survival days of animals. The minimal inhibitory concentrations (MIC) of S. aureus, S. epidermidis, Escherichia coli, Candida albicans were observed by using the tube method Qingwen Jiere. Result: Low and medium doses of Xiaoer Qingwen Jiere granules can increase the lymphocyte proliferation induced by lipopolysaccharide(LPS) and Concanavalin(ConA) significantly. In the model induced by staphylococcus aureus, Xiaoer Qingwen Jiere granules can reduce the mortality significantly and prolong the survival time and inhibite S. aureus(P<0.05,P<0.01), S. epidermidis, E. coli, C. albicans to varying degrees. Conclusion: Xiaoer Qingwen Jiere granule can improve the immune function of immune compromised mice, protect the mice infected by staphylococcus aureus from death in vivo, and inhibit the growth of a variety of bacteria inordinately.  
        关键词：Xiaoer Qingwen Jiere granules;immune regulation;innate immunity;specific immunity;antibacterium   
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      • Vol. 21, Issue 22, Pages: 128-132(2015) DOI: 10.13422/j.cnki.syfjx.2015220128
        摘要：Objective: To observe the effect of Xiaoer Qingwen Jiere granules on the mice models infected by influenza A (H1N1). To observe the in vitro antiviral effect of Xiaoer Qingwen Jiere granules. Method: ICR mice were randomly divided into 7 groups, including normal group, model group, Ribavirin group (82.5 mg·kg-1), Xiaoer Ganmaoning mixture group,Xiaoer Qingwen Jiere granules high, middle, low dose groups (12.8, 6.4, 3.2 g·kg-1), n=10-12 in each group.In respect of in vivo antiviral aspect, ICR mice infected by influenza A (H1N1) were used as pneumonia models. Xiaoer Qingwen Jiere granules (12.8, 6.4, 3.2 g·kg-1) were given since the time of modeling, with Ribavirin as the positive drug to observe the lung inflammation, calculate lung index, death rate,and life prolongation rate.Serum cytokines tumor necrosis factor α (TNF-α), nitric oxide (NO), and γ interferon (IFN-γ) were detected by ELISA. In respect of in vitro antiviral aspect, CEP method was used to observe the pathological changes of virus. Result: Compared with the model group, Xiaoer Qingwen Jiere granules could significantly decrease the lung index and death rate of ICR mice and prolong their life span. Xiaoer Qingwen Jiere granules middle and low dose groups could significantly increase serum INF-γ, and NO levels in infected mice (P<0.05, P<0.01).In respect of in vitro antiviral aspect, Xiaoer Qingwen Jiere granules can significantly suppress pathological changes by H1N1 influenza A virus(FM1 strain, PR8 strain, Brisbane/59/2007), H3N2 influenza A virus (Brisbane/10/2009 strain),influenza B virus(jiangxi xiushui/32/2009 strain), parainfluenza virus 1(PIV-1), and coxsackie virus B type 4 (CoxB4). Conclusion: Xiaoer Qingwen Jiere granules has obvious therapeutic effect for the viral pneumonia caused by influenza A virus in mice, and also has certain inhibition effect on multiple viruses in vitro.  
        关键词：Xiaoer Qingwen Jiere granules;influenza virus;viral pneumonia   
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      • SONG Li-hua,LIU Shu-zhi
        Vol. 21, Issue 22, Pages: 133-136(2015) DOI: 10.13422/j.cnki.syfjx.2015220133
        摘要：Cataplasm is a new type of preparation for external use and belongs to transdermal drug delivery system.It has many advantages,such as high drug loading,good compatibility with skin,minimal skin irritation.Cataplasm is not only used to cure surgical diseases but also internal diseases,it is pasted on the skin surface and can reduce side effects,so it is more and more favored by the pharmaceutical industry.Cataplasm has become a research hotspot.Research materials about cataplasm is concluded,analyzed and summed up by referring the data in recent years, its character,study of the matrix formulation and preparation,quality control and assessment,transdermal evaluation,pharmacokinetics and forecast.Cataplasm is a new formulation,compared with traditional formulations,cataplasm has special advantages,it will have good market in the future.  
        关键词：cataplasm;matrix;preparation process;quality control   
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      • DU Mao-bo,SHEN Shuo,CHEN Qiang,DU Yan-jiao,YAO Yao,HE Ai-ping,LIANG Li-na,LIU Shu-zhi
        Vol. 21, Issue 22, Pages: 137-139(2015) DOI: 10.13422/j.cnki.syfjx.2015220137
        摘要：Objective: To establish a method of screening solid-type surfactants. Method: When surfactants reached critical micelle concentration(CMC),particle size of systems changed remarkably.we defined three parameters of S1,S2 and E,S1 was size when surfactants concentration was lower than CMC,S2 was size when surfactants concentration was higher than CMC,E=S1/S2.E value was the key to screen surfactants.Particle size and Zeta potential of microemulsions was determined,its shape was investigated by transmission electron microscope,its irritation was evaluated by rabbit eye irritation test. Result: Relative strength of emulsifying ability was in the order of cremophor RH40> Kollicoat IR> PVA> β-CD> PEG4000> SLS> poloxamer407> poloxamer188> arabic gum> PEG-CHO modified gelatin B> bone gelatin B.Microemulsions was prepared with cremophor RH40 as surfactant,its particle size was 38.50 nm,Zeta potential was-18.13 mV,its shape was round up and uniform.This microemulsion had no irritation in male rabbit eyes for 7 days. Conclusion: This approach to screen surfactants is reasonable and feasible.  
        关键词：particle size;surfactants;critical micelle concentration;cremophor RH40;poloxamer   
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      • XIE Ming
        Vol. 21, Issue 22, Pages: 140-144(2015) DOI: 10.13422/j.cnki.syfjx.2015220140
        摘要：Formulae and compounds are two concepts with different connotations in traditional Chinese medicine(TCM). Formulae are the main form of using TCM based on the theory of drug properties, syndrome pathogenesis and therapeutic method, and characterized by the combination of TCMs, which fully reflects the close relationship between TCM and TCM herbs. But as the product of TCM modernization, compounds are characterized by the combination of effective parts or components of TCMs based on theory of active substances, disease pathology and pharmacology, which shows the relations between natural medicines and TCMs. There are essential differences in the connotation between formulae and compounds although their similarity in the use of TCMs. The confusion of the definitions of formulae and compounds makes theoretical difficulties for TCM formulary, clinical application base, compounds' efficacy evaluation and application based on syndrome differentiation. TCM formulae and compounds shall have their own research objectives and fields based on different subject backgrounds, connotations and functions. For one thing, modern research of formulae shall follow the experience background in the treatment based on syndrome or disease differentiation and the correlation between the disease syndrome and formulae, and focus on the correlation of drug compatibility and disease or syndrome, namely the regularity of the correlation between the disease syndrome and formulae and the biological connotation. Meanwhile, based on the formula chemistry and its complicated regulating effect, and the identification of formulae's chemical components, efforts shall be made to explore the compound-biological effect mode, which is academically significant in identifying TCM formulae's efficacy principle form a new perspective; For another, compound studies are not necessarily restricted to the experience background in TCM. It is of more practical significance to track the progress in systematic biology and synthetic biology, set up the biological effect evaluation system for the combination of TCM components and discover the new formulae containing TCM chemical fractions, components or ingredients for treating modern diseases by using TCM chemical analysis and the latest separation technology.  
        关键词：formulae;compounds;connotation;traditional Chinese medicine   
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      • ZHANG Jing-sheng,WANG Xiao-wan,ZHANG Guang-xia,LIU Jin-na,XIE Ming,YANG Jian-feng
        Vol. 21, Issue 22, Pages: 145-149(2015) DOI: 10.13422/j.cnki.syfjx.2015220145
        摘要：Objective: To explore the safety concentration of berberine in vitro and its insulin resistance (IR) on adipocyte. Method: The 3T3-L1 preadipocytes were taken into blank control and different concentrations of berberine (0.001~10 μmol·L-1)groups. The A value of each group was measured by MTT method after 48 hours. Mature 3T3-L1 preadipocytes were divided into control and IR model group. The cell culture medium and glucose content were detected after the model group cells were inducted by 10 μmol·L-1 insulin for 24 h and 48 h. Glucose content in the culture medium of the IR model were detected after intervention for 24 h and 48 h. Result: Compared with the control group, the A values of three 0.01-1 μmol·L-1 berberrine groups increased and 10 μmol·L-1 group increased and decreased significantly (P<0.05,P<0.01). Compared with the normal group, the glucose content of culture medium induced by Ins for 24 h and 48 h increased and decreased significantly (P<0.01). Compared with the model group, the glucose content in culture medium of 0.01-1 μmol·L-1 berberine groups after 24 h and 0.0001-1 μmol·L-1 berberine groups after 48 h decreased significantly (P<0.01). Conclusion: The safety concentration of berberine in vitro is low, and the low concentration of berberine in vivo has a significant anti-IR activity.  
        关键词：berberine;3T3-L1 preadipocyte;insulin resistance   
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      • LU Fang,YANG Xiao-dan,JING Yue-e,ZHANG Na,LIU Shu-min
        Vol. 21, Issue 22, Pages: 150-153(2015) DOI: 10.13422/j.cnki.syfjx.2015220150
        摘要：Objective: To observe the effect of Acanthopanax senticosus polysaccharides(ASPS) on endogenous metabolites in normal rats using metabolomics method, find potential biomarkers, analyze the metabolic pathways, and explore its pharmacological effects and mechanisms of action. Method: Twenty SD rats were randomly divided into two groups, namely 10 in the blank group with normal saline treatment (10 mL·kg-1) and 10 in ASPS group with ASPS treatment (100 mg·kg-1) for 20 days respectively. Urine samples were collected on day 21 for UPLC-Q-TOF/MS analysis. Micromass Markerlynx software was adopted for chromatographic peak identifying and peaks matching, and the principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (O-PLS-DA) were used for dimension reduction process for the complex multi-dimensional data. Based on the metabolic fingerprinting data analysis of urine samples in blank group and ASPS group, biomarkers were matched according to mass to charge ratio, and their metabolic pathway was identified. Result: Twenty potential biomarkers such as 6-dimethylaminopurine and L-acetylcarnitine were identified, and their pharmacological activity was found to be related to neuroprotection and resisting cell apoptosis. Conclusion: ASPS has a certain pharmacological effects on Alzheimer's disease, cardiovascular disease and other diseases, and its mechanism may be related to cell apoptosis and excitotoxic hypothesis.  
        关键词：Acanthopanax senticosus polysaccharides;metabonomics;biomarkers;Alzheimers disease;cardiovascular disease   
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        发布时间：2024-01-04
      • REN Yan-dong,JING Yue-e,ZHANG Shu-xiang,WANG Hong-yu,LU Fang,LIU Shu-min
        Vol. 21, Issue 22, Pages: 154-157(2015) DOI: 10.13422/j.cnki.syfjx.2015220154
        摘要：Objective: To investigate the effect of Baichanting compound(BCT) on neuroethology,oxidative stress response, and dopamine (DA) content in mice with Parkinson's disease(PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), and discuss the possible mechanism of BCT's neuroprotection effect in PD models. Method: Male C57BL/6 mice were processed with MPTP-HCl (30 mg·kg-1·d-1, ip, 0.9% normal saline solution) for 5 days to establish PD mice models;the mice were randomly divided into normal group,model group, levodopa and benserazide tablets group(62.50 mg·kg-1·d-1), BCT high-dose, middle-dose and low-dose groups (363.00, 181.50, 95.75 mg·kg-1·d-1). All treatment groups were dissolved with 0.5% carboxyl methyl cellulose (CMC-Na);BCT compound was ig given once daily for 20 days.The pole and independent activity experiments were done to observe the mice behavior change.The content of DA in corpus striatum was detected by UPLC-MS-MS method.Superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px), monoamine oxidase-B (MAO-B),and malondialdehyde (MDA) levels in midbrain substantia nigra tissues were detected by chemical colorimetry method. Result: Compared with the normal group, pole climbing time was significantly longer in model group(P<0.05), autonomic activity significantly reduced(P<0.05), content of DA in corpus striatum, SOD and GSH-Px levels significantly reduced (P<0.01), MDA and MAO-B levels significantly increased (P<0.01). Compared with the model group, neural behavior was improved significantly(P<0.05), the content of DA in corpus striatum,the activity of SOD and GSH-Px were increased significantly(P<0.01,P<0.01),and MDA and MAO-B levels were significantly reduced (P<0.01) in PD model mice after the treatment of BCT. Conclusion: BCT can significantly improve neurobehavioral change and the content of striatal DA in PD mice by improving the body's ability to resist oxidation and scavenging free radicals.It is the possible mechanism of neuroprotective potential for BCT in PD model.  
        关键词：Baichanting;Parkinson's disease;oxidative stress;1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine   
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        发布时间：2024-01-04
      • HONG Xiao-hua,REN Jian-xun,WANG Yang-hui,DONG Xiao-xia,AN Xiao-jing,YANG Bin,JIN Long,GE Zheng-yan,LIU Jian-xun
        Vol. 21, Issue 22, Pages: 158-162(2015) DOI: 10.13422/j.cnki.syfjx.2015220158
        摘要：Objective: To study the effect of Qizhu granule on liver function in rats with adriamycin nephropathy. Method: SD rats were randomly divided to six groups:blank group, model group, prednison group 10 mg·kg-1 and Qizhu granule groups with the dosage of 5, 10, 20 g·kg-1. All animals, except for blank group, had been intravenously injected with drugs to establish the rat model with adriamycin nephropathy. Four weeks later, the serum albumin and lipid levels, liver and kidney functions were measured at the end of the study. The pathological analysis of hepatic and kidney tissues was performed by hematoxylin and easin(HE) staining. The expression of fibronectin (FN) was also measured by immunohistochemical staining. Result: Compared with blank group, the total cholesterol(TCH) and triglycerides(TG) levels in serum and the expression of FN in kidney were significantly increased in model group after the 4-week study (P<0.01), but the serum albumin levels, liver and kidney functions were significantly decreased (P<0.01). Meanwhile, abnormal pathological changes of hepatic and kidney tissues could be observed by HE staining. Compared with model group, Qizhu granule could significantly decrease the levels of TCH and TG in serum and FN expression in kidney (P<0.05, P<0.01), increase the serum albumin levels, improve liver and kidney functions and attenuate pathological changes of hepatic and kidney tissues. Conclusion: Qizhu granule can mitigate hypohepatia in rats with adriamycin nephropathy by long-term administration.  
        关键词：Qizhu granule;liver function;adriamycin nephropathy   
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        发布时间：2024-01-04
      • QIN Hai-jiao,LIU Meng-ting,TANG Li-ying,ZHAN Hao,FANG Jing,YANG Hong-jun,FU Mei-hong,WANG Bin,WU Hong-wei,XIE Lian-wu
        Vol. 21, Issue 22, Pages: 163-168(2015) DOI: 10.13422/j.cnki.syfjx.2015220163
        摘要：Objective: To identify intestinal adsorption ingredients of Epimedii Folium and investigate their absorption characteristics in different intestinal segments. Method: Everted intestinal sac model was utilized.Intestinal sac fluid samples at different time after administration of high,medium and low concentration of Epimedii Folium extract were collected and five flavonoids in fluid samples were determined by HPLC.Accumulative absorbed doses(Q) and absorption rate constants(Ka) of five flavonoids were calculated,while proportions of different flavonoid between Epimedii Folium extract and intestinal absorption liquid were compared. Result: Epimedin A,epimedin B,epimedin C,icariin and icarisid Ⅱ were identified and could be quantitatively analyzed in extract and intestinal sac fluid samples of Epimedii Folium.Q of five flavonoids reached maximum value in front and middle jejunum segments under medium and low concentrations of Epimedii Folium extract,while Q of five flavonoids reached maximum value in ileum under high concentrations of extract.In these three concentrations of Epimedii Folium extract,absorption model of five flavonoids was linearity with R2>0.9,which was in consistent with zero order absorption rate.Ka of five flavonoids increased with increasing of concentration of Epimedii Folium extract,which indicated that intestinal adsorption mechanism of five flavonoids was passive diffusion.Proportions of five flavonoids in intestinal sac fluid were different from those in Epimedii Folium extract. Conclusion: Epimedin A,epimedin B,epimedin C,icariin and icarisid Ⅱ in Epimedii Folium extract can be absorbed in whole intestine.Everted intestinal sac model can be used to evaluate intestinal absorption characteristics of ingredients in Epimedii Folium extract effectively.  
        关键词：Epimedii Folium;everted intestinal sac method;epimedin A;icariin;icarisid Ⅱ   
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        发布时间：2024-01-04
      • WANG Chun,LIU Li-mei,SONG Zhi-qian,DONG Yun-zhuo,DU Zhi-yong,NING Zhang-chi,LIU Yuan-yan,LIU Zhen-li
        Vol. 21, Issue 22, Pages: 169-175(2015) DOI: 10.13422/j.cnki.syfjx.2015220169
        摘要：We searched CNKI,Wanfang data,PubMed and reference lists of identified articles(As of September 1,2014),and reviewed literature,summarized extraction and separation technology of effective components in the most common Chinese medicines for cardiovascular disease.The most common extraction methods were refluxing extraction,ultrasonic extraction,microwave extraction,supercritical extraction,enzyme method,flash extraction and so on,but these researches were usually for laboratory scale.Single factor design,orthogonal design,uniform design and etc were widely used for optimization of extraction method.Water and different concentration of ethanol were always used as extraction solutions.The most common separation methods were macroporous resin,solvent extraction and precipitation method,and the later two methods usually used with macroporous adsorption resin.Some new separation techniques,such as ultrafiltration method,biological transformation technology and molecular imprinting technique,were also used.This article can provide a literature basis for extraction and separation method of Chinese medicines in cardiovascular disease.  
        关键词：cardiovascular disease;Chinese medicine component;Salviae Miltiorrhizae Radix et Rhizoma;purification process;text mining   
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        发布时间：2024-01-04
      • ZHAO Zhi-qiang,BAO Cheng-xian,ZHU Li-hua,JIA Li-xia,LIU Li,ZHANG Qing-bo,LI Xiang-jun
        Vol. 21, Issue 22, Pages: 176-179(2015) DOI: 10.13422/j.cnki.syfjx.2015220176
        摘要：Objective: To study the effect of paeoniflorin on proliferation and cytokine secretion for mouse spleen cell in vitro, in order to determine its impact and mechanism on immune function in vitro for clinical application and industrial production. Method: Spleen cells were cultured with the paeoniflorin solutions at concentrations of 62.5, 12.5, 250, 500, 1000 mg·L-1. The blank group was set as well. Cell culture plates were taken out at 3, 6, 9 d. Proliferations of the cells were determined by counting the cells on the blood cell plates. Interleukin-12(IL-2), interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) secreted by the cells were examined with enzyme linked immune sorbent assay (ELISA). After intervention with 500 mg·L-1 paeoniflorin solution for 5 days, the cell subtypes were checked with flow cytometry (FCM). After intervention for 9 days, changes in cell phenotypes were measured by Western blot. Result: At 3, 6, 9 d, compared with the blank group, proliferation of the cells at different concentrations in drug group were significantly higher (P<0.01), and IL-2, IFN-γ and TNF-α contents in drug group increased significantly with the rise in the concentration of paeoniflorin (P<0.05, P<0.01). Moreover, paeoniflorin could stimulate the proliferation of T lymphocytes and B lymphocytes. Paeoniflorin at different mass concentrations increased the protein expression of IL-2, IFN-γ, TNF-α and nuclear factor-κB (NF-κB). Conclusion: Paeoniflorin can increase immune function by stimulating proliferation of and secretion of IL-2, IFN-γ and TNF-α and activating NF-κB.  
        关键词：paeoniflorin;cellular immune;humeral immune;cytokines   
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        发布时间：2024-01-04
      • LI Xiao-lin,ZHANG Shun-jie,LI Ying,MENG Li-ming,XU Xue-liang,CHEN Min
        Vol. 21, Issue 22, Pages: 180-183(2015) DOI: 10.13422/j.cnki.syfjx.2015220180
        摘要：Objective: To establish an appropriate sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) method for identification of Acanthopanax senticosus and A. sessiliflorus seeds. Method: Using 15% gel SDS-PAGE,soluble proteins in A. senticosus seeds from 15 different locations and A. sessiliflorus seeds from 2 different locations were analyzed.Difference of protein band numbers,band intensity and protein molecular weight were compared by BandScan 5.0 software. Result: Total protein bands of 17 different seed samples were 31,10 common bands and their own specific band of two seeds could be standard protein fingerprints of A. senticosus and A. sessiliflorus seeds.There were some differences in protein bands among A. senticosus seeds from various locations. Conclusion: This electrophoresis method and the standards protein fingerprints can be applied to identify seeds of A.senticosus and A. sessiliflorus.  
        关键词：Acanthopanax senticosus;A. sessiliflorus;protein;SDS-PAGE;electrophoretogram   
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        发布时间：2024-01-04
      • JING Xiao-yang,LEI Yan,XIU Cheng-kui
        Vol. 21, Issue 22, Pages: 184-187(2015) DOI: 10.13422/j.cnki.syfjx.2015220184
        摘要：Objective: To observe the effect of extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma (GNC)on immune organ function and behavior of aging rats. Method: Totally 100 SD (Sprague Dawley) rats were selected to raise for 18-19 months to establish the model of natural aging rats. According to the horizontal stratification of weight, the model aging rats were randomly divided into 5 groups:aging model group, traditional Chinese medicine low-dose, medium-dose, high-dose groups (2305.64, 1152.82, 576.41 mg·kg-1) and resveratrol group (50 mg·kg-1). And another group of 2 month old SD rats was set up as young group. After 3-month intervention, the total number of cell crossing and the total behavior distance of rats were recorded through open field test. Rat serum reactive oxygen species (ROS) content, thymus index and spleen index were detected. Result: Compared with young group rats, the total number of cell crossing decreased in aging model group (P<0.01), the total action distance of natural aging rats decreased (P<0.05), serum ROS content increased (P<0.01), and thymus index and spleen index decreased (both P<0.01). Compared with the aging model group, the number of cell crossing and total action distance of all traditional Chinese medicine groups and resveratrol group increased (P<0.05). The serum content of ROS decreased (P<0.05). Traditional Chinese medicine high-dose group and resveratrol group showed increase in the thymus index and spleen index (P<0.01), the traditional Chinese medicine medium-dose group showed increase in the rat thymus index (P<0.05). Conclusion: The extracts from GNC can improve the mental retardation state of natural aging rats and enhance their space exploration capacity, improve the function of immune organs and reduce the serum ROS content of aging rats.  
        关键词：Extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma;aging;immune;behavior;reactive oxygen species   
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        发布时间：2024-01-04
      • XU Feng,WANG Luo-lin,SHI Zhi-qi,CHEN Peng,SUN Dong-mei
        Vol. 21, Issue 22, Pages: 188-191(2015) DOI: 10.13422/j.cnki.syfjx.2015220188
        摘要：Objective: To optimize preparation process of Zuojin gastric floating-bioadhesive pellets and investigate its in vitro release characteristics. Method: With release of berberine hydrochloride as indicator to evaluate and optimize formulation.Prescription composition for pellet core of Zuojin gastric floating-bioadhesive pellets was optimized by single factor tests,process parameters of extrusion spheronizatiaon were investigated by orthogonal design while pellets circularity and yield of pill core were used as evaluation index.Pellet core were coated by mixed solution of carbomer and sodium bicarbonate. Result: Optimum preparation process was as follows:extrusion speed of 30 r·min-1,rounding speed of 800 r·min-1,spheronization time of 20 min.All coated pellets which were drifted in 1 min were prepared with HPMC K4M as matrix,MCC PH301 as ball material and disintegrating agent,sodium bicarbonate as drift material.About 80% of pellets were continuous floated within 10 hours,biological adhesion rate was 83.7%-86.8%.Cumulative release rate of berberine hydrochloride in 12 hours was more than 90%. Conclusion: Zuojin gastric floating-bioadhesive pellets has good release properties with uniform appearance,its preparation technology is simple with low production cost.  
        关键词：Zuojin Wan;gastric floating;bioadhesive;extrusion spheronization method;turnadle pan coating;berberine hydrochloride   
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        发布时间：2024-01-04
      • TANG Shi-huan,LU Peng,YANG Hong-jun
        Vol. 21, Issue 22, Pages: 192-196(2015) DOI: 10.13422/j.cnki.syfjx.2015220192
        摘要：As a kind of functional food, health food is a kind of functional food, which has a long history in our country and has a unique advantage, and it is an important part of our national health care industry. Following the arrival of the big health era, health food industry will usher in an unprecedented development opportunities. To speed up the development of health food, this paper has constructed a structured database of health food formulations based on Traditional Chinese Medicine Inheritance System (TCMISS), and made a systematic analysis of the formulation about the 26 health functions of formula data. On the basis of this, the research on the method of association rules is used to select the most common raw materials for each health function. This study has identified the various health care functions corresponding formula commonly used raw materials and formulation features.From the new perspective of health food formulations the prescription composition rules, the results can provide a basis and reference for the selection of raw materials and formula research and development of health food.  
        关键词：health food;Traditional Chinese Medicine Inheritance System;database;raw materials;health food formulation   
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        发布时间：2024-01-04
      • WU Jia-rui,GUO Wei-xian,LIN Meng-juan,ZHANG Dan,ZHANG Xiao-meng,MA Wen-hao,ZHANG Yue,ZHANG Bing
        Vol. 21, Issue 22, Pages: 197-202(2015) DOI: 10.13422/j.cnki.syfjx.2015220197
        摘要：Objective: To explore the medication rules of state medical master YAN Zheng-hua's prescriptions with Taxilli Herba. Method: Prof. YAN Zheng-hua's prescriptions containing Taxilli Herba were collected to build a database based on Traditional Chinese Medicine Inheritance Support System (TCMISS). Then the data were mainly analyzed by Apriori algorithm in TCMISS to acquire the frequency of single medicine, the frequency of drug combinations, the association rules and core drug combinations. Result: The prescriptions containing Taxilli Herba were commonly used to treat vertigo, arthromyodynia syndrome, lumbago and other syndromes. The frequently used drugs were Paeoniae Radix Rubra, Achyranthis Bidentatae Radix, Paeoniae Radix Alba, Salviae Miltiorrhizae Radix et Rhizoma, Dipsaci Radix, and so on. The frequently used drug combinations were 'Paeoniae Radix Rubra, Taxilli Herba', 'Achyranthis Bidentatae Radix, Taxilli Herba', and 'Paeoniae Radix Alba, Taxilli Herba'. The drug association rules with a confidence of more than 0.9 included 'Achyranthis Bidentatae Radix-> Taxilli Herba','Poria-> Taxilli Herba', 'Mori Ramulus-> Taxilli Herba', and 'Fired Ziziphi Spinosae Semen-> Taxilli Herba', and so on. Conclusion: Prof. YAN Zheng-hua's prescriptions containing Taxilli Herba mostly have the effects of nourishing liver and kidney, promoting blood circulation to remove blood stasis and relieve pain, expelling evil-wind, removing dampness, with concentrated medication and clear prescription principles.  
        关键词：Taxilli Herba;data mining;association rules   
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        发布时间：2024-01-04
      • WU Jia-rui,GUO Wei-xian,LIN Meng-juan,ZHANG Xiao-meng,ZHANG Dan,ZHOU Wei,ZHANG Bing
        Vol. 21, Issue 22, Pages: 203-208(2015) DOI: 10.13422/j.cnki.syfjx.2015220203
        摘要：Objective: To explore the medication rules of State Medical Master YAN Zheng-hua's prescriptions with Achyranthis Bidentatae Radix. Method: The prescriptions including Achyranthis Bidentatae Radix that built by Pro. Yan were collected to build a database based on Traditional Chinese Medicine Inheritance Support System (TCMISS). Then the data were analyzed mainly by Apriori algorithm in the data analysis module of TCMISS to acquire the frequency of single medicine, the frequency of drug combinations, the association rules and core drug combinations in 441 prescriptions including Achyranthis Bidentatae Radix. Result: The prescriptions including Achyranthis Bidentatae Radix were commonly used to treat vertigo, rheumatism syndrome, lumbago and other syndromes. The highest frequency used drugs were Paeoniae Radix Rubra, Taxilli Herba, Paeoniae Radix Alba, Salviae Miltiorrhizae Radix et Rhizoma, Leonuri Herba, and so on. The most frequency drug combinations were 'Achyranthis Bidentatae Radix, Paeoniae Radix Rubra', 'Achyranthis Bidentatae Radix, Taxilli Herba', and 'Paeoniae Radix Rubra, Taxilli Herba' and so on. when the the minimum-confidence was 0.9, the drug association rules were 'Dipsaci Radix-> Achyranthis Bidentatae Radix', 'Paeoniae Radix Alba, Taxilli Herba-> Achyranthis Bidentatae Radix', 'Paeoniae Radix Alba, Paeoniae Radix Rubra, Taxilli Herba-> Achyranthis Bidentatae Radix', and 'Taxilli Herba-> Achyranthis Bidentatae Radix', and so on. Conclusion: The drugs in the prescriptions containing Achyranthis Bidentatae Radix that built by Pro. Yan mostly had the effects of promoting blood circulation to remove meridian obstruction, invigorating the liver and kidney and strengthening muscles and bones, which reflected the clear and precise conception of composing formulas.  
        关键词：Achyranthis Bidentatae Radix;data mining;association rules   
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        发布时间：2024-01-04
      • ZHANG Shuo,WU Jia-rui,ZHANG Xiao-meng,ZHANG Dan,JI Kai
        Vol. 21, Issue 22, Pages: 209-213(2015) DOI: 10.13422/j.cnki.syfjx.2015220209
        摘要：Objective: To investigate the medication rules of traditional Chinese Medicine (TCM) for treating impotence. Method: The prescriptions for treating impotence that from TCM classics and famous TCM doctors were collected to build a database based on TCM inheritance assist system. Association rules algorithm and entropy clustering methods were used to mine the frequency of single drug and drug combinations, the association rules between drugs, and so on. Result: Based on the analysis of prescriptions for treating diabetes, the highest frequency of single drugs were Cuscutae Semen, Rehmanniae Radix Praeparata, Angelicae Sinensis Radix, Lycii Fructus, and Epimedii Folium. The most frequency drug combinations were 'Lycii Fructus, Cuscutae Semen', 'Cistanchis Herba, Cuscutae Semen', 'Lycii Fructus, Epimedii Folium'and so on. When confidence was over 0.85, the drug combinations of association rules were 'Poria->Cuscuta Semen', 'Rehmanniae Radix Praeparata->Lycii Fructus', 'Bupleuri Radix->Angelicae Sinensis Radix', and so on. Conclusion: In TCM, the drugs used for treating impotence were often with the actions of warming and invigorating kidney Yang, and nourishing Qi and blood.  
        关键词：impotence;famous traditional Chinese medicine doctor;association rules;clustering algorithm   
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        发布时间：2024-01-04
      • WU Jia-rui,WANG Kai-huan,JI Kai,ZHANG Dan,ZHANG Xiao-meng,YANG Bing,FENG Run-bin
        Vol. 21, Issue 22, Pages: 214-217(2015) DOI: 10.13422/j.cnki.syfjx.2015220214
        摘要：Objective: To explore the medication rules of traditional Chinese medicine for treating diabetes. Method: The traditional Chinese medicine (TCM) prescriptions for treating diabetes were collected to build a database based on TCM inheritance assist system. Association rules algorithm and entropy clustering methods were used to determine the frequency of single drug and drug combinations, the association rules between drugs, core drug combinations and so on. Result: Based on the analysis of prescriptions for treating diabetes, the frequently adopted drugs were Astragali Radix, Poria, Rehmanniae Radix, Dioscoreae Rhizoma, and Salviae Miltiorrhizae Radix et Rhizoma. The frequently adopted drug combinations were 'Salviae Miltiorrhizae Radix et Rhizoma and Astragali Radix', 'Astragali Radix and Rehmanniae Radix', and 'Poria and Astragali Radix'. In the association rules with a confidence of over 0.85, the drug combinations included 'Corni Fructus, Moutan Cortex, Poria->Dioscoreae Rhizoma', 'Moutan Cortex, Alismatis Rhizoma, Poria->Dioscoreae Rhizoma', and 'Dioscoreae Rhizoma, Moutan Cortex, Alismatis Rhizoma->Poria'. Conclusion: In TCM, the drugs used for treating diabetes mostly have the effects of tonifying Qi and Yin, strengthening spleen and kidney, clearing heat and promoting fluid, and activating circulation to remove blood stasis, which reflects a refined and rational compatibility.  
        关键词：diabetes;association rules;clustering algorithm   
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      • SONG Jing-mei,WU Jia-rui,JIANG Di,ZHANG Dan
        Vol. 21, Issue 22, Pages: 218-221(2015) DOI: 10.13422/j.cnki.syfjx.2015220218
        摘要：Objective: To investigate the medication rules of traditional Chinese medicines(TCM) for the treatment of hepatopathy by National Medical Masters. Method: The prescriptions for hepatopathy were collected to build a database based on TCM inheritance assist system. Apriori algorithm and complex system entropy cluster were adopted to determined the frequency of single medicine and drug combinations that used in prescriptions, and the association rules between drugs e. Result: Frequently used herbs included Poria, Salviae Miltiorrhizae Radix et Rhizoma, Artemisiae Scopariae Herba, Atractylodis Macrocephalae Rhizoma and Bupleuri Radix;Frequently used drug combinations included 'Salviae Miltiorrhizae Radix et Rhizoma and Poria', 'Atractylodis Macrocephalae Rhizoma and Poria' and 'Salviae Miltiorrhizae Radix et Rhizoma and and Paeoniae Radix Rubra'.The association rules with a confidence of above 0.6 included 'Astragali Radix-> Salviae Miltiorrhizae Radix et Rhizoma and', 'Atractylodis Macrocephalae Rhizoma-> Poria', 'Astragali Radix-> Poria' and 'Pilosula Codonopsis-> Poria'. Conclusion: The prescriptions used for the treatment of hepatopathy mostly have the effects of reinforcing spleen and eliminating dampness, cooling blood and activating blood circulation, nourishing Yin to calm liver and discharging liver and relieving depression, with the refined compatibility and the TCM medication principles for treating symptoms and root causes.  
        关键词：hepatopathy;association rules;clustering algorithm   
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        发布时间：2024-01-04
      • WU Jia-rui,YANG Shu-yi,ZHANG Xiao-meng,ZHANG Dan,LIU Shi,ZHANG Bing,YANG Bing
        Vol. 21, Issue 22, Pages: 222-225(2015) DOI: 10.13422/j.cnki.syfjx.2015220222
        摘要：Objective: To systematically evaluate the clinical efficacy and safety of Shengmai injection in the treatment of angina pectoris in coronary heart disease. Method: Literatures concerning randomized controlled trials(RCTs) regarding Shengmai injection in the treatment of angina pectoris in coronary heart disease were searched in CNKI, VIP, WanFang Database, CBM, PubMed, Embase and Cochrane Library. Quality of the included studies was evaluated with the method recommended in Cochrane, extracted the required information, and statistical was performed using the Review Manager 5.2 software. Result: A total of 14 RCTs with 932 participants were included, and the experimental group was 472 cases and the control group was 460 cases. Clinical effect analysis:Firstly, 9 studies included compare the total clinical effective rate, the Meta-analysis indicated that Shengmai injection combined with conventional treatment with western medicine is effective in treating angina pectoris in coronary heart disease, on the basis of conventional therapy, Shengmai injection's total clinical effective rate is higher compared to the control group, there were statistically significant differences between groups[RR=1.24, 95%CI(1.17,1.32), P<0.00001]. Secondly, 3 studies compared Hs-CRP and the Meta-analysis indicated that Shengmai injection combined with conventional treatment with western medicine can reduce Hs-CRP effectively[MD=-4.46, 95%CI(-6.79,-2.12), P=0.0002]. Finally, 2 studies compared the rheology of blood and the Meta-analysis indicated Shengmai injection combined with conventional treatment with western medicine can reduce rheology of blood effectively[MD=-0.11, 95%CI(-0.18,-0.03),P=0.006]. Conclusion: Shengmai injection combined with conventional treatment with western medicine is effective in treating cardiovascular and cerebrovascular diseases.  
        关键词：Shengmai Injection;angina pectoris in coronary heart disease;systemic evaluation   
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        发布时间：2024-01-04
      • WU Jia-rui,ZHANG Yu-jing,ZHANG Xiao-meng,ZHANG Dan,ZHANG Bing,YANG Bing,JIN Yan-ping,FENG Run-bin,SHENG Xiao-guang
        Vol. 21, Issue 22, Pages: 226-228(2015) DOI: 10.13422/j.cnki.syfjx.2015220226
        摘要：Objective: To explore the rules of compatibility of the antagonistic combination of 'Zingiberis Rhizoma and Scutellariae Radix' in clinical. Method: The prescriptions included the application of 'Zingiberis Rhizoma and Scutellariae Radix' were systematically retrieved in databases, and the collation and analysis of related diseases, dosage, compatibility proportion and preparation formulation were analyzed by Excel of Microsoft 2007. Result: The prescriptions included the antagonistic combination of 'Zingiberis Rhizoma and Scutellariae Radix' were mainly used for the treatment of internal diseases (878 cases, 90%), which was followed by surgical diseases (52 cases, 5%), gynecological diseases (17 cases, 2%), and so on. In the internal diseases, the quantity of morbidity of spleen-stomach diseases was the most, which was accounted for 80% (705 cases) of the total. Others were diseases of pulmonary system (63 cases, 7%), and diseases in the liver and gallbladder (46 cases, 5%).In the prescriptions, the dosages of Radix Scutellariae were primarily 10,9,6,15 g, 82% of which were in the range provided by Chinese Pharmacopoeia. The dosages of Zingiberis Rhizoma were mainly 10 g and 6 g, 95% of which were in the range provided by Chinese Pharmacopoeia. And the compatibility proportion of 'Zingiberis Rhizoma and Scutellariae Radix' was mostly 1 to 1, which was accounted for 36%. Conclusion: The antagonistic combination of 'Zingiberis Rhizoma and Scutellariae Radix' was widely used in a variety of interior, exterior, gynaecology, paediatrics and other syndromes. The application of the antagonistic combination was proved have a satisfactory efficacy, though the two drugs have the contrary property.  
        关键词：antagonistic theory;Scutellariae Radix;Zingiberis Rhizoma;compatibility   
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        发布时间：2024-01-04
      • TANG Lin,HE Qian,LI Li,MENG Jing-yan
        Vol. 21, Issue 22, Pages: 229-233(2015) DOI: 10.13422/j.cnki.syfjx.2015220229
        摘要：Objective: To analyze the rules of Ye Tian-shi's prescriptions for treating stomachache in Ye Tian-shi's Medical Records by using traditional Chinese medicine inheritance support system (TCMISS). Method: The prescriptions for treating stomachache in YE Tian-shi's Medical Records were collected and then entered into TCMISS. The medication rules were analyzed by using data mining methods, including revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering. Result: ① Based on the analysis of 105 prescriptions, 20 herbs were used for more than 10 times in the database. ② 19 herb pairs were used for 11 times, when the support degree was set to 11.Moreover, when the confidence was set to 0.6, the association rules for the herbs in the prescriptions were obtained. ③ 14 pairs of new core herbal combinations and 7 new prescriptions were obtained by using entropy cluster and hierarchical clustering methods. ④ The similarity of 20 prescriptions including Jinlingzi power was searched by matching prescriptions. Conclusion: The rules of Ye Tian-shi's prescriptions for treating stomachache were summarized through TCMISS, which will provide the data support for in-depth studies on Ye's prescriptions.  
        关键词：Ye Tian-shi;stomachache;traditional Chinese medicine inheritance support system;data mining;rules of prescriptions   
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        发布时间：2024-01-04
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