最新刊期
卷 17 ， 期 3 ， 2011
- 摘要：Objective: Establishing the ambiexponent equation of pogostone cataplasm transdermal data. Method: The pogostone concentration in reception liquid was determined by GC at different time point and the data was fitted by different model. Result: The ambiexponent equation was the best fitting model. Conclusion: The ambiexponent equation was a suitable fitting model in drug transdermal absorption.关键词：pogostone cataplasm;transdermal absorption;ambiexponent equation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the release properties of geranium tannins colon-specific preparation in vitro . Method: The pellets were prepared by using calcium pectinate as a carrier, Geranium tannins as the model drug to fill in the intestines dissolving capsule. The release of the preparation in different mediums in vitro was determined with gallic acid as the index. Result: The accumulated percentage of release rate of pellets in simulated gastrointestinal fluid was less than 10%, but in the simulated colonic fluid which contained 0.5% pectin enzyme, the accumulated percentage of release rate reached 80% within 4 hours. Conclusion: The preparation had the characteristics as a colon-specific delivery system.关键词：calcium pectinate;colon-specific;compound
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发布时间：2024-01-04 - 摘要：Objective: To study the preparation technique of chrysophanol microcapsules. Method: The preparation technique of chrysophanol microcapsules was optimized by orthogonal design; in vitro release of chrysophanol microcapsules, morphology and its stability were studied. Result: With the complex agglutination method, the encapsulation rate was significantly related to the ratio of coating material, the encapsulation temperature and the stirring speed. The highest rate of production and minimum diameter of microcapsule were obtained when the ratio of coating material to chrysophanol was 1 ∶4, the temperature of encapsulation was at 60 ℃ and the stirring speed was 200 r ·min-1. Conclusion: The process was simple and reliable. The chrysophanol microcapsules were very stable and could be used as sustained-release preparation.关键词：chrysophanol;microcapsule;orthogonal design;preparation technique;in vitro release
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发布时间：2024-01-04 - 摘要：Objective: To select the optimal molding technology of Waiganfengsha sugarless granules. Method: Orthogonal design of L9 (34) was used with composite score of formalizing properties, color uniformity, solubility and moisture percentage as the judgement criteria to screen out the kinds and ratio of the excipients and optimal preparation process. Result: The optimum conditions was A2B2C2 , that was 1.75 times of mixing excipients; the mixed composition of excipients was dextrin-MCC-stevia hormone (94 ∶5 ∶1); the wetting agent was 90% alcohol. The factors to be affected in descending order were A>C>B ; among the factors, A and C of the granulation comprehensive score was significant ( P <0.05). Conclusion: The molding technology was stable and reasonable, and can be applied to industrial production.关键词：Waiganfengsha sugarless granules;orthogonal experiments;composite score;molding technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction process of the total flavones of Lithocarpus litseifolius . Method: Main effective component of phloridzin in total flavones was taken as indexes, and extraction process was optimized by comparative experiment and orthogonal test. Result: The optimum extraction process was determined as follows: extracting three times by refluxing, 10-fold drug amount and 70% alcohol added and extracted for two hours. Conclusion: The extraction process is scientific, reasonable and feasible.关键词：Lithocarpus litseifolius;total flavones;phloridzin;extraction process
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发布时间：2024-01-04 - 摘要：Objective: Four different macroporous resins of D101, AB-8, DM130 and ADS-7 were studied by the test of capability of static adsorption, static desorption and dynamic desorption according to the index of ephedrine. The concentration of ephedrine was determined by HPLC. Macroporous resin of D101 was the optimal absorbent for purifying Mahuang Fuzi Xixinting with static saturation time of 1 hour, static absorption capability of 999.5 μ g·g-1, static desorption rate of 90.5% and dynamic desorption rate of 94.1%.关键词：Mahuang Fuzi Xixin Tang;ephedrine;Macroporous resin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction technology for total alkaloids in the root of Aconitum flavum . Method: Dissolvent, times of extraction and washing were the test factors and the total alkaloids were the test index. The orthogonal design was used to optimize the extraction technology of total alkaloids. Result: The optimal extraction method was as follows: the ether:chloroform (3 ∶1) as dissolvent, extraction 3 times and washing once. Conclusion: The technology after optimization had a good stability, the yield of total alkaloids was high, and the method was easy to operate.关键词：Orthogonal Design;total alkaloids of Aconitum flavum;extraction technology;acid-base titrations
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发布时间：2024-01-04 - 摘要：Objective: To optimize the best matrix’s prescription and the techniques of preparation of Tianmagouteng cataplasm. Method: The matrix of Tianmagouteng Cataplasm was studied by uniform design and taking adhesive strength and cohesive strength as evaluation indices. Result: The best ingredient of the matrix was polyacylic acid Na-carbopol 940-PVP30-aluminium chloride-kaolin-citric acid-glycerin(6 ∶2 ∶0.5 ∶0.2 ∶4 ∶0.25 ∶45). Conclusion: Tianmagouteng Cataplasm with this precription had great capacity of loading extractive and good viscosity.关键词：Tianmagouteng cataplasm;cataplasm;matrix;uniform design
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发布时间：2024-01-04 - 摘要：Objective: To probe the effects of Rhizoma Corydalis processed with different acids on both the content of tetrahydropalmatine and the analgesia by Rhizoma Corydalis. Method: The decoction amount of the tetrahydropalmatine was measured by HPLC. The pain threshold was measured separately before and after mice took the drug by means of the hot plate. Result: The different processing of Rhizoma Corydalis exerted a certain influence on the decoction amount of the tetrahydropalmatine. Rhizoma Corydalis and its different acid products had an analgesic effect on the mice causing pain by hot plate. Conclusion: Of all the acid products of Rhizoma Corydalis, acetic acid ones had the least satisfactory effect. The comparison between malic acid, hydrochloric acid products and acetic acid product was not clearly marked. The citric acid products and the tartaric acid products had a certain advantage over others.关键词：Rhizoma Corydalis;processing with acid;tetrahydropalmatine;analgesic effect
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发布时间：2024-01-04 - 摘要：Objective: To optimize the separation and purification of total phenolic acids from salvia by polyamide resins. Method: Based on absorptive capacity and elution efficiency of total phenolic acids in salvia, the conditions to separate and purify total phenolic acids in salvia were determined. Result: The polyamide resin (60-100 meshes) was chosen, washed with 40% ethanol, and the optimal concentration was 3.5 mg ·mL-1. The purity of total phenolic acids obtained was 98.41%. Conclusion: Using polyamide resin (60-100 meshes) to separate and purify total phenolic acids from salvia is feasible.关键词：salvia;total phenolic acids;polyamide resins;separation and purification
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发布时间：2024-01-04 - 摘要：Objective: Studies on extraction and purification technology of the Chinese medicine leeches, preparing process of extracting leeches to provide technical basis. Method: The extraction of leeches was optimized by orthogonal experiment and countercurrent circuit extractive technique, with titer detected by anti-thrombin activity as an index, and using of membrane separation technology to refine extract. Result: The best technology was to extract five hours with 12 times the amount of 0.9% sodium chloride solution, and extract three times. Selected membrane with 30×１０３ and 0.5×１０３ could achieve the purpose of the purification. Conclusion: The extraction and purification technology was stable, reasonable and practicable.关键词：Leech;countercurrent circuit extractive technique;membrane separation technique;purification;determination of tobramycin by turbidimetric method
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction technology of naringin from Citrus aurantium. Method: The content of C. aurantium was determined by HPLC. The extraction technology was optimized by orthogonal experiment with the extraction rate and the extract of naringin as evaluation index. Result: The optimum extraction technology was determined as follows: 10 times amount of 90% ethanol was used as solvent, the extraction time was 1 hour and the extraction was conducted for 3 times. Conclusion: The extraction technology was simple and reliable. It can be used as the optimal extraction of naringin from C. aurantium.关键词：Citrus aurantium;extract;naringin
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发布时间：2024-01-04 - 摘要：Objective: To establish the method of dissolution test for Xuesaitong Dispersible Tablets. Method: The small cup method was used with distilled water as dissolution medium at a rotate speed of 50r ·min- 1, Paddle method was used and the dissolving time was 60 minutes. The dissolution rate was determined by HPLC-ELSD. Result: The cumulate dissolution rate of Xuesaitong Dispersible Tablets had little difference in and between batches. The cumulate dissolution percentage of Xuesaitong Dispersible Tablets tended to be stable after 30 minutes, and the dissolution parameters T50 and Td of Xuesaitong Dispersible Tablets were 3.58min, 5.99min, Xuesaitong capsules and tablets dissolution parameters T50 and Td were 13.39, 25.42, 26.53, 56.77 min respectively. Conclusion: The method established is simple,accurate and repeatable for the quality control of Xuesaitong Dispersible Tablets. The dissolution of Xuesaitong Dispersible Tablets was quicker than that of the marketed capsules and that of the common tablets.关键词：Xuesaitong Dispersible Tablets;dissolution rate;Panax notoginoside;HPLC- ELSD
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction technology of Zhuanshu Granules. Method: L9(34) table was used to examine the effects of the 4 factors, and the weighted sum of the content of ginsenoside, total flavones in Licorice and total polysaccharides were determined by orthogonal test. Result: The optimal extraction process was as follows: 10 times of 30% alcohol was added and reflux extraction was performed 3 times for 1.5 h each time. Conclusion: The optimized extraction process was reasonable in design, stable in results and can provide theoretic support for industrialization of Zhuanshu Granules.关键词：Zhuanshu Granules;Extracting Technology;orthogonal experiment
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发布时间：2024-01-04 - 摘要：Objective: To establish the optimized preparation of Simotang dropping pills. Method : The optimum extraction process was selected with Single-factor test and orthogonal, taking difference of pill mass,dissolution and the shape of pill as indicators, and through examing the dropping temperature, dropping speed and external diameter of burette to select the best dropping conditions. Result: The best dropping conditions was that the dropping temperature was at 75 ℃,dropping speed was 30 to 40 drops per minutes, internal and external diameter of burette are 2.0 mm and 5.0 mm. Conclusion: The formulation technology is suitable for industrial production.关键词：Simotang dropping pills;orthogonal testing;formulation technology
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发布时间：2024-01-04 - 摘要：Objective: To screen and optimize the extract technique of Yiganzhuanyin Granules. Method: The extract technique of Yiganzhuanyin Granules was investigated using orthogonal design with the dry extract yielding rate and quercetin content as the index. Result: The optimal condition for the extraction of Yiganzhuanyin Granules was 10 folds amount of water,3 times,1 hour each time. Conclusion: The optimum extraction procedure is stable and reliable,and can be used in the extraction procedure of Yiganzhuanyin Granules.关键词：Yiganzhuanyin Granules;quercetin;extract technique;orthogonal design
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发布时间：2024-01-04 - 摘要：Objective: To optimize the decolorization technology of the extract of Nervilia fordii . Method: The loss of free amino acids and decolorization of the extract of N fordii were used as index. On the base of single factor screening, the effects of the pH value, the amount of activated carbon, decolorizing temperature and decolorizating time were investigated. The uniform design was adopted for getting the optimal mixed technique parameters, and verified by experiment. Result: The pH value of extract solution was 3.0; the activated carbon added was 0.01 g \5L-1; tempetature was at 40 ℃, and decolorizing time lasted for 20 min. Conclusion: The optimized extraction process is stable and feasible.关键词：Nervilia fordii;amino acid;decoloration
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发布时间：2024-01-04 - 摘要：Objective: To analyze the fatty acid and other fat-soluble components in seeds of Semen Plantaginis. Method: The seeds of Semen Plantaginis was smashed and screening by 40 of mesh sieve, and extracted by using petroleum ether (60~90 ℃).The extractionwas concentrated and deal by the methylated method. After that, the fatty acids and other-fat-soluble components were determined by GC-MS, and used area normalization method for quantitative analysis. Result: 50 substances were detected from the petroleum etherextraction of seeds of Semen Plantaginis that identified 42 compounds,25 kinds of fatty acids and 17 non-fatty acids. Conclusion: The major fatty acids are 9,12,15-(Z,Z,Z)-octadecatrienoic acid(30.09%),9,12-(Z,Z)-octad-ecadienoie acid(25.91%),13-(Z)-docosenoic acid(7.60%),14-methyl-pentadecanoic acid(7.41%) as well as the rare fatty acids of 2-hexyl-cyclopropaned-ecanoic acid(6.51%),2-octyl-cyclopropaneoctanoic-acid(0.60%)and 2- cyclopropyl]methyl]-cyclopropaneoctanoic acid(0.07%).关键词：seeds of Semen Plantaginis;fatty acids;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To research the effects of different concentrations of trace elements zinc on yield and effective components of Rabdosia rubescens under field condition,and to provide scientific basis for proper application of trace elements zinc. Method: Foliar application of different concentrations of trace elements zinc In the initial stage of growth and development of R. rubescens，according to the yield and content of the results of analysis of oridonin ,rosmarinie aci and ponicidin，filtering out the best spraying concentration. Result： Compared with spraying water, 0.10% of the zinc could make the R. rubescens high yields and improve its effective components，increase yields 16.5%,enhance the content of oridonin 40.28%. Conclusion ：Rational application of zinc could increase the yields and made an obvious improvement in the content of oridonin.关键词：冬凌草;锌;产量;有效成分
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents from the petroleum ether and chloroform fractions of ethanol extract from Spiraea pubescens . Method: The compounds were isolated by silica gel chromatography and preparative HPLC,and their structures were elucidated on the basis of chemical and spectroscopic methods,including MS,1D and 2D NMR spectral techniques. Result: Six compounds were isolated from the petroleum ether and chloroform fractions of ethanol extract from S . pubescens ,and were identified as lupeone(1),friedelin(2),lupeol(3), β -amyrin(4),betulin(5),and uvaol(6). Conclusion: This is the first report of the isolation of 1-5 from Spiraea genus,and 6 from this plant.关键词：Spiraea pubescens;pentacyclic triterpenoid;chemical constituent
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发布时间：2024-01-04 - 摘要：Objective: Study on variation of ester alkaloids and hydrolysate in Radix Aconite Lateralis with the change of decocting time to establish the relationship between the content change and decocting time. Method: HPLC analysis was performed on a Eclipse XDB C18 (4.6 mm ×250 mm,5 μm) with 0.1 mol ·L-1 ammonium acetate (contains 0.5 mL glacial acetic acid per 1 000 mL, phase A) and acetonitrile-tetrahydrofuran (25 ∶15, phase B) as mobile phase. Gradient elution was used to the determination of ester alkaloids in Baifupian and crude aconite roots at different decocting time. Result: The diester-type alkaloids in crude Aconite roots were extremely unstable, and there was only hypaconitine detected in 0.5 hours. But it's three kinds of monoester-type alkaloid showed a tendency of increasing gradually with increase of decocting time, and reached the peak at 8 hours. The mesaconitine and hypaconitine in Baifupian were detected in the whole decoction process, but the aconitine did't detect after 4 hours of decoction. The benzoylmesaconine and benzoylaconine in Baifupian decocted fluid firstly increased and then decreased, after 3-5 hours reached the peak, while the benzoylhypaconine gradually increased to the peak within 10 hours. Conclusion: In the decoction process, the change of ester alkaloids in crude Aconite roots and Baifupian was significantly different. The general trend is that the diester-type alkaloids in crude Aconite roots and Baifupian have transformed into pymesaconitine, pyraconitine, pyrhypaconitine, benzoylmesaconine, benzoylaconine and benzoylhypaconine.关键词：Radix Aconite Lateralis;decocting;ester alkaloids;dynamic variation
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发布时间：2024-01-04 - 摘要：Objective: Study on extract method of liposoluble constituent bufalin, cinobufagin and resibufogenin in Bufons Venenum by orthogonal experiment design and established HPLC method for determination the assay of three effective constituents above. Method: An orthogonal test was adopted in this study. The content of bufalin, cinobufagin and resibufogenin was used as index for optimizing the extraction condition. In established HPLC method , the column:Diamonsil-C\-18 column(4.6 mm×250 mm,5 μm),mobile phase:acetonitrile-water (55 ∶45), the flow rate :1.0 mL·min-1,the detection wavelength 296 nm. Result: The best extraction technology was to extract for 120 min with 75 times 95% ethanol in 80 ℃. The calibration curves of bufalin, cinobufagin, resibufogenin were linear and LOD were 14.6,9.0,11.8 ng ·mL-1 respectively. The repeatability and precision of the HPLC method was good. Conclusion: Confirmed the best extract method and established HPLC method to determine the assay of three constituents in Bufons Venenum extraction was simple and accurate.关键词：Bufons Venenum;orthogonal experiment design;bufalin;cinobufagin;resibufogenin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for HPLC-fingerprint of Radix Aconiti from different areas. Method: The fingerprint for Radix Aconiti from different areas was established by HPLC. Gemini-NX C18 column was used at 35 ℃ with acetonitrile-0.2% acetic acid (adjusted to pH 10.0 with ammonia water) as mobile phase by gradient eluting, the flow rate was set at 0.8 mL ·min-1, detection wavelength was set at 235 nm and eluting period was 75 min. Result: HPLC-fingerprint of R.aconiti from different areas was established, which contained 12 common peaks, and six peaks in the fingerprint were identified, most of the similarities were over 92%, 10 batches of R.aconiti from different areas were classified into three groups based on hierarchical cluster. Conclusion: Fingerprint established was characteristic and specific, combined with the determination it could be used for the quality control of Radix Aconiti .关键词：Radix Aconiti;HPLC;chromatographic fingerprint;clustering analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method to determine the content of adenosine in Pinellia pedatisecta from different districts. Method: Experiment was carried out on Kromasil C18 (4.6mm×200mm, 5μm) column with mobile phase consisted of methanol-water (15 ∶85) at a flow rate of 1.0 mL ·min-1.The detection wavelength was at 260 nm and the column temperature was at 25 ℃. Result: The linear range of adenosine was 9.84-78.72mg ·L-1 (r=0.999 7).The average recovery was 99.01%, RSD 1.94% (n=6). The adenosine content of P. pedatisecta from different districts ranged in order was as follows: Heze>Anguo>Hanzhong>Baoding>Bozhou>Liuyang. Conclusion: The method is quick, simple and reproducible for the determination of adenosine in P. pedatisecta.关键词：Pinellia pedatisecta;adenosine;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the volatile oil from Bulbophyllum odoratissimum . Method: The chemical compositions of the volatile oil of the plants which were obtained by organic-steam distillation with hexane were analyzed by GC-MS. The constituents were identified by their mass spectra. The relative percentage of the oil constituents was calculated from the GC peak areas. Result: Thirty-eight kinds of chemical components were identified from B. odoratissimum. The amount of the identified components was accounted for 91.448% of all the volatile oil. The constituents whose relative content was more than 2% were determined as Z-9-octadecenal (18.075%), 2,4-Di-tertbutylphenol (3.264%), palmitic acid (5.413%), oleic acid (25.079%), tricosane (6.344%), tetracosane (3.768%), 1-Monoolein (2.416%), 1-monolinolein (4.953%), pontacosane (4.332%), hexacosane (3.387%), heptacosane (3.841%), octacosane (3.102%). Conclusion: The composition of volatile oils from B. odoratissimum by GC-MS.关键词：Bulbophyllum odoratissimum;Steam Distillation;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for determination of the content of linoleic acid and α-linoleic acid in hemp fruit by GC, and provide evidence for evaluating quality and criterion. Method: The GC separation was performed on a ZB-WAX column (0.5 μm×0.32 mm×30 m) at 190 ℃, inject temperature at 250 ℃, FID detecter temperature was at 250 ℃, gas as N2 (99.99%); flow rate was 1.0 mL ·min-1, air 450 mL ·min-1, H2 45 mL ·min-1. Result: The content of linoleic acid and α-linoleic acid in hemp fruit was 31.42%-37.21%, 8.02%-9.65%. Conclusion: The method can be used to control the quality of linoleic acid and α-linoleic acid in hemp fruit, which is accurate, convenient and repeatable.关键词：hemp fruit;linoleic acid;α-linoleic acid;GC
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for determination of berberine hydrochloride and chlorogenic acid in Zhizi jinhuawan by capillary electrophoresis. Method: The separation was performed on a fused silica capillary of 60 cm×75 μm ID (52 cm of effective length). 0.2 moL ·L-1 NaH2PO4 +CH3CH2OH(50 ∶50)(pH 5.50)and 50 mmol ·L-1 Na2B4O7 were selected as the running buffers. The separation voltages were 21 kV and 12 kV, gravity injection (10 s, 15 cm). The detection wavelengths were 265 nm and 327 nm. Result: The concentration ranges of determination for berberine hydrochloride and chlorogenic acid were 11.0-44.0 mg ·L-1 and 3.6-14.4 mg ·L-1 with linear ralationship ( r = 0.999 3). The average recoveries were 100.0% and 98.1%, precisions of the method were 1.72% and 0.96%(RSD, n = 6). The average content of berberine hydrochloride was 3.39 mg ·g-1, chlorogenic acid was 0.88 mg ·g-1. Conclusion: The method was accurate, sensitive and rapid for the quality control of Zhizi Jinhuawan.关键词：capillary electrophoresis;Zhizi Jinhuawan;berberine hydrochloride;chlorogenic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of Berberine hydrochloride in Qingfei Yihuo Wan. Method: The analysis was performed on a Diamonsil-C18 column using acetonitrile-water (54 ∶46) (every 100 mL add in 0.34 g SDS and 0.17 g NaH2PO4,pH adjusted to 3.0 with phosphoric acid) as mobile phase. The flow rate was 1.0 mL ·min-1, and the detection wavelength was 350 nm. Result: The linear range of berberine hydrochloride was 5.00-35.00 mg ·L-1 ( r =0.999 2). The average recovery for berberine hydrochloride was 97.6%, precision of the method was 1.39% ( n =6). Conclusion: The method can be used for quantitative determination of Berberine hydrochloride in Qingfei Yihuo wan.关键词：Qingfei Yihuo wan;berberine hydrochloride;Assay;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish the methods for determining protocatechuic acid, chlorgenic acid and caffeic acid in Sangye dispensing granules by HPLC. Method: The column of Hypersil ODS2 C18 column (4.6 mm× 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution. The flow rate was 1.0 mL ·min-1. The column temperature was at 20 ℃, the detection wavelength was at 258 nm, 328 nm. Result: The average recovery of protocatechuic acid, chlorgenic acid and caffeic acid was 102.1%, 101.1% and 101.5% respectively. Protocatechuic acid, chlorgenic acid and caffeic acid were linear in the range of 0.032 2-0.161 0 μg, 0.125 6-1.256 μg and 0.035 1-0.351 μg respectively. Conclusion: The method is simple, accurate and can used to determine the content of the protocatechuic acid in mulberry formula granules.关键词：Sangye dispensing granules;HPLC;protocatechuic acid;chlorgenic acid;caffeic acid
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents of essential oil from the leaves and flowers of Pittosporum tobira Ait in Guangxi. Method: The essential oils were extracted by steam distillation and analyzed by GC-MS. The relative content was determined by area normalization. Result: Ten compounds were identified, which accounted for over 100% of the leaves essential oils. The main constituents were viridiflorol (40.09%), hexadecane (15.13%). 6 compounds were identified, which accounted for 100% of the flowers essential oils. The main constituents were viridiflorol (60.33%), 4-methoxy-6-(2-propenyl)-1,3-benzodioxole (17.40%), ledol (5.69%). Conclusion: The main constituents of essential oil from the leaves and flowers of P. tobira in Guangxi is viridiflorol.关键词：Pittosporum tobira Ait;essential oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To elucidate the constituentes from the whole plant of Elephantopus scaber and to provide samples for activity screening. Method: The chromatography on silica gel was used and the structures were determined by IR, NMR and MS spectra analysis and physicochemical properties comparison. Result: Eight compounds were isolated and identified as 3,4-dihydroxy benzaldehyde(1), p -coumaric acid(2), vanillic acid(3), syringic acid(4), β -stitosterol(5), daucosterol(6), 2,5-dimethoxy-1,4-benzoquinone(7), n- octacosanoic acid(8). Conclusion: The compounds 1,3,5,7 and 8 were isolated from the plant scaber for the first time.关键词：Elephantopus scaber;chemical constiuents;2,5-dimethoxy-1,4-benzoquinone;syringic acid
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发布时间：2024-01-04 - 摘要：Objective: These volatile components were extracted from 5 species flowers of Michelia figo , Magnolia liliflora,Gardenia jasminoides , Agastache rugosa , and Rosa multiflora , this research will provide scientific proof for the search of anti-oxidation activities and antimicrobial activities to empolder these natural resources. Method: These volatile components were extracted from flowers of M. figo , M. liliflora , G. jasminoides , A. rugosa , and R. multiflora respectively by steam distillation. Five species volatile components were investigated about antimicrobial activities of scavenging effect for ·OH, and were evaluated to anti-oxidation activities using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. Result: Five species volatile components have obvious action to anti-oxidation activities of scavenging effect for ·OH, and inhibited the growth of testing bacterias and sterilized them. Conclusion: Their anti-oxidation activities showed M. figo ﹥ A. rugosa ﹥ M. liliflora ﹥ G. jasminoides ﹥ R. multiflora . The antimicrobial results showed A. rugosa ﹥ M. liliflora ﹥ R. multiflora ﹥ G. jasminoides ﹥ M. figo .关键词：flower;volatile component;anti-oxidation activity;antimicrobial activity
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method to determine the concentration of methotrexatate (MTX) in human plasma. Method: The ferulic acid was used as inter standard (I.S.) The analytical column was a Kromasil C18 column connected with the Agilent 1100 HPLC system. The isocratic mobile phase was the acetonitrile and buffer of phosphate acid (pH 6.8) with the rate of 15 ∶85.The detective UV wavelength was 306 nm and the column temperature was 30 ℃. The plasma samples, added ferulic acid as internal standard, were deposited down the protein with acetonitrile and the upper phase was evaporated by nitrogen at 60 ℃. The remains was dissolved with mobile phase, 20 μL was injected into the sampler. Result: The chromatography was good and not interfered by the components of the plasma. The linear equation was Y=-1.65×10-2X+0.355,r=0.999 5. Conclusion: This is a accurate, sensitive and conventient method for the dertermination of MTX in the therapeutic drug monitoring.关键词：Methotrexate;HPLC;acute lymphoblasticleukemia
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard for Shaoshi Securing capsule. Method: Paeonia lactiflora and Catsia tora were identified by TLC. The content of paeoniflorin in Shaoshi Securing capsule was determined by HPLC. HPLC analytical method was carried out by using a C18 column and a mixture of methanol-water (27 ∶73) as the mobile phase. The detection wavelength was set at 230 nm. Result: Spots obtained from the test solutions had the same color in reference solution and medical material in the same location, and the bland solution had no interference. The calibration curve was linear in the range of 0.074-0.741 μg ( r =0.999 95). The average recovery of sample was 100.7% with RSD of 2.0% ( n =6). Precision of the analytical method was 1.1% ( n =6). Conclusion: In TLC the spots were very clear and specific to identify the herbal medicine in Shaoshi Securing Capsule. HPLC method is simple, quick, high precise and accurate for the quality control of the capsules.关键词：Shaoshi Securing capsule;TLC;HPLC;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To study the regulatory effect of Liuwei Dihuang Pill (LDP) and Jingui Shenqi Pill (JSP) on cytokines expression in the brain of experimental autoimmune encephalomyelitis (EAE) mice. Method: The EAE model was induced by subcutaneous injection of myelin oligodendrocyte glycoprotein (MOG)35~55 and complete Freund’s adjuvant (CFA), supplemented with intraperitoneally pertussis toxin (PTX). The brain of mice was removed on day 12th, 25th and 40th post-immunization (PI), respectively. And the protein expression of TNF-α, TGF-β and IL-17 were assessed by immunohistochemistry method. Result: Compared with the normal control (NC), the protein expression of TNF-α significantly increased in model group on day 25th PI (P<0.01). Compared with the model group, TNF-α expression decreased in LDP-treated and JSP-treated group significantly (P<0.01). The protein expression of TGF-β in model group significantly decreased compared with NC mice on day 40th PI (P<0.01). Compared with model group, TGF-β protein increased in prednisone acetates (PA), LDP and JSP-treated groups significantly (P<0.05 or P<0.01). The protein expression of IL-17 in model group significantly increased compared with the NC group on day25th and 40th PI (P<0.01). Compared with model group, IL-17 protein reduced in LDP-treated and JSP-treated groups (P<0.01). The ratio of TNF-α/TGF-β and IL-17/TGF-β significantly increased compared with the NC mice on day 40th PI (P<0.01), but reduced in PA, LDP and JSP-treated groups(P<0.01). Conclusion: Both LDP and JSP treatment may suppress EAE through regulating the cytokines of TNF-α, TGF-β and IL-17.关键词：Liuwei Dihuang pill;Jingui Shenqi Pill;experimental autoimmune encephalomyelitis;tumor necrosis factor-α;interleukin-17;transforming growth factor-β;mice 刘妍,等:补肾方对实验性自身免疫性脑脊髓炎小鼠T辅助细胞1,17和调节性T细胞的影响
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Siteng Fang (STF), a Chinese herb formula, on proliferation and apoptosis in hepatocarcinoma Bel-7402 cells in vitro . Method: Human hepatocarcinoma Bel-7402 cells and human normal liver HL-7702 cells were treated with different dose of STF. Cell proliferation was detected with CCK-8 (cell counting kit-8, CCK-8) assay; cell apoptosis was detected by flow cytometry. Apoptotic morphology was visualized by hoechst 33258 staining, and activation of caspase-3 was detected by western blot assay. Result: STF showed no obvious cytotoxicity against normal liver HL-7702 cells. Upon treatment with 100-400 mg ·L-1 STF for 24 h, proliferation of Bel-7402 cells was significantly inhibited. 100-400 mg ·L-1 STF also induced apoptosis in Bel-7402 cells. In addition, caspase-3 was activated after 100-400 mg ·L-1STF treatment. Conclusion: STF may inhibit cell proliferation, and induce apoptosis in Bel-7402 hepatocarcinoma cells. Activation of caspase-3 may be involved in the process.关键词：hepatocarcinoma cell;Siteng Fang;proliferation;apoptosis;caspases-3
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Anxin Granules (AXG) on the levels of serum superoxide dismutase (SOD) and malondialdehyde(MDA) and myocardial cell apoptosis in rats with chronic heart failure (CHF) ,and to explore the possible therapeutic mechanism. Method: Fifty female SD rats were randomly divided into five groups:normal control group (A),model group (B),captopril group (C),low-dose AXG group (D) and high-dose AXG group (E).CHF models were established by intraperitoneal injection of small-dose of adriamycin,once per week for continuous 6 weeks.Meanwhile,group C was injected captopril (6.25 mg ·kg-1·d-1); groups D and E received low-dose AXG (0.8 g ·kg-1·d-1) and high-dose AXG (1.6 g ·kg-1·d-1) ig respectively.After 6 weeks,serum levels of SOD, MDA and the left ventricular mass index (LVMI) of different groups were detected. Myocardial cells apoptosis was tested by TUNEL. Apoptosis index (AI) was counted. Result: Compared to group A,the serum levels of MDA ,and LVMI,and AI in cardiac muscle were markedly increased in the model group (P<0.01),the activity of SOD in serum was significantly decreased (P<0.01) in rats model with CHF. AXG decreased the serum levels of MDA,LVMI and AI compared with the model group (P<0.05 or P<0.01).And the level of SOD was ascended markedly. Conclusion : AXG inhibit apoptosis and anti-oxidative stress.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Chaihu Shugan Tang on EEG and expression of multidrug resistance protein P-gp of refractory epilepsy rats induced by licl-pilocarpine. Method: Except for blank control group(A),50 Sprague-Dawley refractory epilepsy rats were randomly divided into five groups: model group(B group),VPA group(C group),low,middle,high dose of Chaihu Shugan Tang groups (D,E,F group). Chaihu Shugan Tang was decocted and concentrated into 1g crude drug/mL. C group is administrated for 0.2 g ·kg-1, D,E,F group is 4.5,9,18 g ·kg-1,the same amount of saline was given to A,B group. After the intragastirc administration for 8 weeks each group, EEG was recorded and the protein P-gp was detected in rat hippocampus and temporal cortex in each group. Result: There was no epileptic wave in A group,but much more epileptic wave in B group. Epileptic wave were decreased in C,D,E,F groups compared with B group. Expression of P-gp by Western blotting was different in each group ( P <0.01). Conclusion :VPA increases expression of multidrug resistance protein P-gp in rat hippocampus and temporal cortex. Chaihu Shugan Tang decreases expression of P-gp and epileptic wave. High dose of Chaihu Shugan Tang is the most efficient.关键词：refractory epilepsy;traditional Chinese medicine;electroencephalogram;multidrug resistance;P-glycoprotein;Chaihu Shugan Tang
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Tengcha extract(TF)on hepatofibrosis induced by chronic hepatic injury. Method: Wistar rats were randomized into six groups: TF treatment (200, 100 and 50 mg ·kg-1) groups, colchicines treatment(Colc,0.1 mg ·kg-1)group, model group and normal control group. Rats were injected carbon tetrachloride (CCl4) subcutaneously two times a week to induce hepatofibrosis as well as ig administration of TF or colchicines. After 10 weeks, the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST), alkaline phosphatase(AKP), albumin(ALB), globulin (GLB) in serum and hydroxyproline(Hyp), malondialdehyde(MDA), glutathione peroxidase(GSH-Px) in liver tissue were determined, and liver histological changes were investigated by hematoxylin-eosin(HE) staining. Result: Compared with model group, TF significantly reduced the levels of ALT, AST, AKP and GLB in serum(P<0.01),and decreased Hyp, MDA level in liver tissue (P<0.05)while ALB, A/G, GSH-Px were elevated significantly(P<0.01). Pathological examination revealed that TF had therapeutical effect on experimental hepatofibrosis. Conclusion: TF has better effects on protecting hepatocyte, relieving liver injury and against hepatofibrosis.关键词：Tengcha;hepatofibrosis;carbon tetrachloride(CCl4)
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发布时间：2024-01-04 -
Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells
摘要：Objective: To study the influence of CLYQ on proliferation and extracellular matrix(ECM) of hepatic stellate cells(HSC). Method: Cytotoxicity caused by CLYQ was detected by MTT assay. The contents of hydroxyproline (Hyp), hyaluronic acid (HA) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in HSC cell supernate were detected by enzyme linked immunoabsorbent assay (ELISA). Result: TC50 is 6.191 g ·L-1, and TC0 is 3.504 g ·L-1. CLYQ could inhibit the proliferation of HSC. The various concentration group of CLYQ could degrade significantly the contents of Hyp, HA and TIMP-1 in cell supernate (P<0.05, P<0.01), presenting the dose-effect relationship. Conclusion: CLYQ can inhibit the proliferation of HSC, so that reducing the synthesis of ECM and hepatic fibrosis.关键词：Compound Liuyueqing (CLYQ);hepatic fibrosis;hepatic stellate cell;cell proliferation;extracellular matrix- 3
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Zhongfeng Zaobei Huatan Jiaonang(ZFZBHTJN) on nerve protection and the VEGF expression in cerebral ischemia-reperfusion injury rats model. Method: Fifty SD rats were randomly divided into sham operation group, ischemia-reperfusion (I-R) group, ZFZBHTJN high, Medium, low dose group(1.62,1.08,0.54 g \5kg-1 \5d-1). The rat model of cerebral ischemia-reperfusion was produced by Zea-longa method. The impacts of ZFZBHTJN on neuro logic function and expression of VEGF were obseved. Result: Compared with sham operation group, I-R group presented severe neuro logic deficits and increases of VEGF expression in cortex and hippocampus.Compared with those in I-R group, neuro logic deficits of rats were relieved significantly and VEGF expression increased distinctly in ZFZBHTJN groups. High dose group (1.62 g \5kg-1 \5d-1) presented the most significant effect on nerve protection (P<0.01). Conclusion: ZFZBHTJN may relive cerebral ischemia-reperfusion injury in rats by increasing VEGF expression.关键词：Zhongfeng Zaobei Huatan Jiaonang(ZFZBHTJN);cerebral ischemia-reperfusion injury;vascular endothelial growth factor;rat model
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发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-inflammatory effect of Sedum lineare Thunb(SLT). Method: Three models were used to explore the anti-inflammatory effect of SLT. Two acute inflammatory models were made in mice, one was ear edema induced by d imethylbenzene, and the other was higher permeability of blood capillary induced by acetic acid. The chronic inflammatory model of granulation tissue was established by cotton pellets implantation in mice. Result: SLT suppressed the swelling of earflaps induced by dimethylbenzene, decreased the permeability of blood capillary induced by acetic acid obviously, and it significantly inhibited the proliferation of granulation tissue. SLT also decreased thoracic gland index and enhanced the spleen index in chronic inflammatory model. Conclusion: The SLT has obvious anti-inflammatory effect, which may result from modulating immune function.关键词：Sedum lineare Thunb;anti-inflammatory effect;mice
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of the Shenfu Yixin Granule on heart failure rats myocardial c-fos,c-myc expression. Method: Hear failure rats were produced by left anterior descending coronary artery occlusion in one hundred thirty heathly male SD rats. Twenty rats obtained the surgery without ligation as sham control. After 8 weeks, rats with left ventricle ejection fraction(LVEF)<50% were randomly Yixin assigned to 5 groups (n=10 in each group): model group, captopril group, losartan group, two groups of Shenfu Yixin Granule(5.48,21.90 g ·kg-1 ·d-1). All the medicine was taken orrally once a day for 4 weeks. The rats in model group and sham control (n=10) were administered equivalent water. After 4 weeks, The LVEF was valued with Color Doppler Ultrasound again. The left ventricular mass index (LVMI) was checked. The expression of c-fos,c-myc were detected respectively by immune-histochemisty technique. Result: Shenfu Yixin Granule(21.90 g ·kg-1 ·d-1) increased the LVEF, decreased LVMI and inhibited the expression of c-fos,c-myc. Conclusion: Shenfu Yixin Granules inhibit the expression of c-fos,c-myc in heart failure rats.关键词：Shenfu Yixin Granula;heart failure;c-fos;c-myc
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发布时间：2024-01-04 - 摘要：Objective: To explore the protection effects of Gastrodia elata on cerebral ischemia-reperfusion. Method: The model of focal cerebral ischemia-reperfusion in rats was established by the suture-occlusion method. One hundred and forty four Wistar rats were randomized into sham group,model group,G. elata extraction low dose group (50 mg ·kg-1), G. elata extraction medium dose group(100 mg ·kg-1),G. elata extraction high dose group (150 mg ·kg-1),Nimodipine group(25 mg ·kg-1). Every group were randomized again into 3 sub-groups at 6 hours,12 hours and 24 hours after ischemia-reperfusion, and rats of sub-groups were killed at different time points respectively. Consecutive coronal sections were done to Hippocampal CAI. Neuronal apoptosis were detected by immunohistochemical method and caspase-8 expression was detected by TUNEL method. Result: ① Compared with the sham group, at time points of 6 hours,12 hours and 24 hours after reperfusion, neuronal apoptosis of model group was significantly increased(P<0.05); Compared with the model group, neuronal apoptosis of G. elata extraction groups,Nimodipine group were significantly decreased(P<0.05;P<0.01)after reperfusion.② Compared with the sham group, the caspase-8 protein expression of model group was significantly increased after reperfusion(P<0.05;P<0.01); Compared with the model group, the caspase-8 protein expression of G.elata extraction groups,Nimodipine group was significantly decreased(P<0.05;P<0.01)after reperfusion. G. elata reduced the level of the caspase-8 protein expression,attenuating neural apoptosis. Conclusion: G. elata has a protectetive effect on cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis.关键词：Gastrodia elata;cerebral ischemia/reperfusion injury;neuronal apoptosis;caspase-8
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发布时间：2024-01-04 - 摘要：Objective: To explore effects of Ganoderma lucidum polysaccharides(GLP) on synapsis and hippocampal synaptophysin expression in AD-rats. Method: AD-rats induced by Aβ25-35 were treated with different concentrations of GLP for 7 days. The Morris water maze was applied to validate the learning and memory ability in rats. The expression of synaptophysin and the alleosis of synaptic stereology were detected on tissues of CA1 and CA3 regions in hippocampus by immunohistochemitry method, transmission electron microscope (TEM) and image-analysis system. Result: Compared with the model group, GLP (75,50 mg ·kg-1) decreased the average delitescence of rats in Morris water maze, and GLP increased the synaptophysin expression levels (P<0.01)and numerica density(Nv),surface density(Sv) (P<0.01,P<0.01). Conclusion: GLP improves the learning ability in AD-rats. Reversal of synaptophysin and Nv, Sv on hippocampus could be contributed to the mechanism.关键词：alzheimer disease;β-amyloid polypeptide;hippocampus;synaptophysin;Ganoderma lucidum polysaccharides
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influences of extract from Peony Radix Alba on the behavioral and hypothalamic-pituitary-adrenocortical (HPA) axis changes in rats with damaged olfactory bulb (DOB). Method: The tests of open-field and step-down passive avoidance were used to observe the behaviors of rats. Radioimmunoassay (RIA) was used to analyze the level of corticotropin releasing hormone (CRH) in hypothalamus, adrenocorticotropic hormone (ACTH) in pituitary gland and cortisol (CORT) in serum of rats with DOB. Result: The rats had a characteristic hyperactivity in the test of "open-field" and learning deficits in step-down passive avoidance (P<0.05), and their levels of CRH, ACTH and CORT increased significantly (P<0.05). The extract of Peony Radix Alba at the dose of 70,35 mg ·kg-1 corrected the behavioral changes (P<0.05) and decreased the levels of CRH, ACTH and CORT (P<0.05). Conclusion: The extract of Peony Radix Alba can correct behavioral changes in rats with DOB, and its regulating effect on HPA axis is one of the mechanisms for treating depression.关键词：Peony Radix Alba;damaged olfactory bulb;corticotropin releasing hormone;adrenocorticotropic hormone;cortisol
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Anwei decoction on the expression of intestinal trefoil factor mRNAs (TFF3 mRNAs) in chronic atrophic gastritis(CAG) rats. Method: Thirty six rats were randomly divided into three groups: model group, Anwei decoction group and Weifuchun group. CAG was induced by N-methy-N-nit ro-N-nitroguanidine(MNNG). The expression of TFF3 mRNA in rats gastric mucosas was determined by real-time polymerase chain reaction (real-time PCR). Result: The ratio of TFF3 mRNA/β-actin was 0.95±0.23,0.43±0.04,0.64±0.04 respectively in the model group, Anwei decoction group and Weifuchun group. Compared with that in the model group, the expression of TFF3 mRNA was remarkably reduced in Anwei decoction group and Weifuchun group(P<0.01). Anwei decoction showed better effect than Weifuchun(P<0.05). Conclusion: Anwei decoction may treat CAG by reducing the expression of TFF3 mRNA in gastric mucosas.关键词：Anwei decoction;atrophic gastritis;intestinal trefoil factor;experimental research
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Zingiberis Rhizoma Recens on neuron apoptosis in cerebral ischemia-reperfusion injury of rats. Method: Male SD rats were randomly allocated into six groups: sham-operation group, model contral group, nimodipine group, aqueous extract of Zingiberis Rhizoma Recens groups with high, medium or low dose groups. The model of focal cerebral ischemia-reperfusion (MCAO) in rat was produced by Zea Longas thread method. The brains were taken out on the ice for paraffin processing at 24 hours after ischemia-reperfusion. Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL staining) was used to assay neuron apoptosis in cerebral cortex and hippocampus. The protein expressions of caspase-3(cysteine-containing aspartate-specific protease), Bcl-2(B cell lymphoma/leukemia-2) and Bax(Bcl-2 accociated X protein) in hippocampus were detected by immunohistochemistry method. Result: Compared with sham operation group, TUNEL staining positive cells and caspase-3, Bcl-2 and Bax immunoreative neurons in cortex and hippocampal tissue increased significantly, the ratio of Bcl-2/Bax decreased in MCAO rat model control group. Aqueous extract of Zingiberis Rhizoma Recens decreased TUNEL positive cells and caspase-3, Bcl-2 and Bax immunoreative neurons in hippocampal tissue significantly and increased the ratio of Bcl-2/Bax markedly, compared with MCAO rat model control group. Conclusion: Aqueous extract of Zingiberis Rhizoma Recens has protective effects on cerebral ischemia-reperfusion. Apoptosis signal relative proteins may be involved in the protection.关键词：Zingiberis Rhizoma Recens;focal cerebral ischemia-reperfusion;apoptosis;apoptosis-related protein
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of XueluoFuxin granula on carotid artery thrombosis in rats and mesenteric microcirculation disturbance in mice. Method: Wistar rats were randomly divided into six groups according to body weight: normal control group, XueluoFuxin groups (5.2, 10.4,20.8 g ·kg-1 ·d-1 crude herb), Fufang Xueshuantong group (0.4 g ·kg-1 ·d-1) and troxerutin group (48.6 mg ·kg-1 ·d-1). Rats were given drugs ig everyday for 7 days. The right common carotid arteries of anesthetized rats were separated to observe the thromboembolism events at 30 minutes after the last administration. Mice were randomly divided into normal control group, XueluoFuxin groups (7.5,15.0,30.0 g ·kg-1 ·d-1 crude herb), Danlou tablet group (0.58 g ·kg-1 ·d-1) and racanisodamine group (3.9 mg ·kg-1 ·d-1). Mice were given drugs ig for 7 days. Adrenaline was locally given on jejunal loops to induce mesenteric microcirculation disturbance at 30 min after the last administration. Micrangium diameter, blood flow rate in micrangium and hemorheology were observed. Result: XueluoFuxin reduced the thromboembolism events; accelerated the blood flow rate in micrangium and improved hemorheology. Conclusion: XueluoFuxin has the effects of inhibiting thrombosis and improving microcirculation.关键词：XueluoFuxin;thrombus;microcirculation
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects and mechanism of Shuweitang on expression of interstitial cells of cajal(ICC) and motilin(MTL)in functional dyspepsia(FD) rats. Method: FD model was induced by moderate enraging the rats tail. All rats were divided random1y into 6 groups: control group, model group, high and low dosages of Shuweitang groups, domperidone and Muxiangshunqi groups. Drugs were administrated for 14 days. ICC and MTL were determined by immunohistochemical method.And gastric emptying was observed with phenolsu1fonphthalein. Result: Compared with the control group, gastric emptying and the expression of ICC and MTL in the model groups were obviously decreased (P<0.05). Compared with model group, gastric emptying, MTL and ICC increased significantly in high-dose Shuweitang, Muxiangshunqi and domperidone groups (P<0.05).Low dose of Shuweitang showed no significant differences(P>0.05). Conclusion: High dose of Shuweitang may increase diet and water intake, promote ICC and MTL expression in FD rats, and accelerate gastric emptying.关键词：functional dyspepsia;rats;Shuweitang;interstitial cells of Cajal;motilin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Bixue Capsule on hemorheological changes in a rat model of acute blood stasis. Method: Sixty SD rats were divided at random into normal control group, model control group, Tongxinluo Capsule control groups, Low dose group, middle dose groups and high dose group (242,48,968 mg \5kg-1 accordingly) of Bixue Capsule. Rats were treated with oral medication daily for 7 days. The acute blood stasis was induced by subcutaneous injection of large dose of adrenaline hydrochloride and ice-water socking for the rats. Then, the hemorheological parameters were observed for evaluating the effects of Bixue Capsule. Result: As compared with vehicle, Bixue Capsule obviously decreased the low shear viscosity of whole blood, the hematocrit and erythrocyte aggregation (P<0.05). Conclusion: Bixue Capsule significantly reduced the degree of red blood cells aggregation and improved blood viscosity in rats with blood stasis syndrom.关键词：Bixue Capsule;blood stasis syndrom;hemorheology;rat
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发布时间：2024-01-04 - 摘要：Objective: To observe the correlation between the expression of AQP1,AQP5 mRNA and the lung injury in the rats with lung sluggishness syndrome and the regulative effect of rhubarb on it, to explore the material foundation of the lung defensive sluggishness syndrom and the mechanism of rhubarb in the prevention and treatment of the lung injury. Method: Lipopolysaccharide(LPS) was injected into SD rats to perform lung-defensive sluggishness syndrome.Sixty rats was randomly divided into 5 groups:control group,lung-defensive sluggishness group,natural recovery after 5 days group,rhubarb prevention group (9 g ·kg-1 ·d-1 for 2 days before model establishment)and rhubarb treatment group(9 g ·kg-1 ·d -1for 5 days after model establishment). Physiological and pathological staining was performed and real-time PCR was used to observe the expression of AQP1,AQP5 mRNA in the pulmonary tissue. Result: The pulmonary edema could be showed obviously in the lung-defensive sluggishness model and rhubarb could alleviate it. Compared with the control group,expression of AQP1, AQP5 mRNA of the model group was decreased,thus the natural recovery after 5 days group was elevated;no significantly difference can be seen between rhubarb prevention group and rhubarb treatment group. Conclusion: The pulmonary edema and abnormal expression of AQP1,AQP5 mRNA can be showed in lung-defensive sluggishness model and the rhubarb can inhibit them,which is one of the important mechanisms in protecting the lung injury of lung-defensive sluggishness syndrome.关键词：lung-defensive sluggishness;material foundation;lung injury;AQP1,AQP5 mRNA;Rhubarb
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发布时间：2024-01-04 - 摘要：Objective: To observe the post-stroke depression(PSD) model of rat and hippocampal neuronal apoptosis related gene expression changes, and the intervention of Bupleurum Liver-Coursing Powder on for PSD and the molecular mechanism. Method: One hundred male SD rats were randomly divided into the following 3 groups:Bupleurum Liver-Coursing Powder group, PSD model control group and normal control group. Except the normal control group, at first the other two groups were set up middle cerebral artery occlusion(MCAO) rat model, eneurological recovery of stroke rat was assessed by Longa grades standard test. A week after surgery,we re-establish PSD composite rat model, while Bupleurum Liver-Coursing Powder group (7.875 g \5kg-1) was fed 21 days with the drugs, PSD model control group was fed distilled water.Immunohistochemical staining detect Bcl-2 and Bax protein expression in hippoca mpus. Result: Compared with the normal control group, Bax protein expression in PSD model group was increased, but Bcl-2 protein decreased(P<0.01); Compared with the PSD model group, Bupleurum Liver-Coursing powder group was significantly reduced in Bax protein expression (P<0.01) and increased in Bcl-2 protein expression (P<0.01). Conclusion: Bupleurum Liver-Coursing Powder may play a role in the treatment of PSD through inhibiting the apoptosis by means of intervention with reduced Bax protein expression and increased the expression of Bcl-2 gene.关键词：Bupleurum Liver-Coursing Powder;post-stroke depression;hippocampal neurons;gene expression
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发布时间：2024-01-04 - 摘要：Objective: To explore the methods of culturing highly purified rat brain microvascular endothelial cells(BMEC). Method: BMEC of SD rats were prepared by filtering through a mesh,and digested by collagenase. Then the cells were examined under microscope and the expression of factor Ⅷ polyclone antibody was determined. Result: The cells became polygon arranging as single layer in 1-2 days. In 7-10 days, the cells arranged as ovum-like in compact monolayer. Immunocytochemistry of factor Ⅷ polyclone antibody showed positive in cytomembrane and cytoplasm, but negative in cell nucleus. Most of the cells were found to express factor Ⅷ-related antigen. Conclusion: Ideal rat brain microvascular endothelial cells can be cultured by the improved cultural method of double filtering and digestion.关键词：microvascular endothelial cells;cell cultivation;SD rat
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发布时间：2024-01-04 - 摘要：Objective: To study effects of Simo decoction on expression of dopamine receptor 1,2(DR1,DR2) in brain and duodenum tissue in chronic stress mice. Method: The mice were randomly divided into control group, stress only (model) group, mosapride group and Simo decoction oral liquid (7.56 mL \5kg-1) group.Chronic stress model was established by exerting the factors of irregular diet, reverse of day and night, binding and stimulating tails. And then treated with distilled water, domperidone and Simo decoction. The expressions of DR1 and DR2 were measured by immunohistochemistry . Result: Simo decoction significantly enhanced expressions of DR1 and DR2 in brain and duodenum tissue compared with the normal group( P＜0.05).and there was significantly difference with model group P＜0.05). Conclusion: Simo decoction appears potential to regulate the expression of DR1 and DR2 in brain and duodenum tissue, which is one of possible mechanism to therapy FGIDs.关键词：Simo decoction;chronic stress;dopamine receptor 1;dopamine receptor 2
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of compound rehmannia on learning and memory abilities in senile dementia mice induced by D-galactose. Method: The senile dementia model mice were established by D-galactose hypodermic injection for 6 weeks and then given compound rehmannia in therapeutic group and the same volume of normal saline in the control. The learning and memory ability of all mice were test with Y-type maze, Nitric oxide (NO) and Superoxide dismutase(SOD) in brain were determined afterward. Result : Compared with control group, the learning and memory abilities in model group were lowered (P＜0.01). After given compound rehmannia all the abilities were significantly improved (P＜0.01). Conclusion: compound rehmannia can improve the leaning and memory abilities.关键词：D-galactose;senile mice;learning and memory
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Huangjin capsule on insulin resistance in type 2 diabetic rats and the primary pharmacological mechanism. Method: The model of type 2 diabetic-insulin-resistant rats (T\-2DIRR) was made through intraperitonea injection of lower dosage of streptozocin(30 mg ·kg-1) and high caloric diet (abounded with sugar & fat),the effects of Huangjin capsule on weight,blood and serum contents of glucose, TC,TG,HDL-C, observed in the model rats. Result: Huang jin capsule lowered significantly fasting blood glucose (FBG) and improved HDL-C; and reduced the TC,TG in T\-2DM,IR, and reduced the concentration of insulin in hyperinsulinemia,and potentiated ISI (P<0.01) in T\-2DM,IR. Conclusion: In the experiment with type 2 diabetic-insulin-resistant rats,huang jin capsule can lower fasting blood glucose (FBG) and reduce level of TC,TG, and improve HDL-C,insulin sensitivity.关键词：Huangjin capsule;type 2 diabetes mellitus;insulin resistance
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发布时间：2024-01-04 - 摘要：Objective: To study the pharmacokinetics of salidroside liposomes in rats. Method: SD rats were divided into control group with salidroside solution and experimental group with liposomes. Plasma concentrations of salidroside in rats after intragastric administration were determined by HPLC. The pharmacokinetics parameters were calculated by DAS2.0. Result: The main pharmacokinetic parameters of liposomes and solution of salidroside were as the followings:t1/2β were (13.19±0.60), (5.16±0.19)h;AUC0-12were (4.75±0.69) and (2.68±0.74) μg ·mL-1 ·h-1. Conclusion: Salidroside liposomes could release the drug slowly.
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发布时间：2024-01-04 - 摘要：Objective: To observe the expression of nuclear factor-κB (NF-κB)and plasminogen activator inhibitor-1(PAI-1) of the renal interstitial in rat unilateral ureteral obstruction (UUO) model and the renoprotective effect of bicyclol, and then to explore the mechanisms. Method: Renal interstitial fibrosis rat model was produced by unilateral ureteral obstruction, eighty-one rats were randomly assigned to shame operation group, UUO model group and bicyclol-treated group. After operations rats in bicyclol-treated group were intregastric administration(ia) at bicyclol 200 mg ·kg-1 once a day until rats were killed. Rats in sham-operated group and UUO model group were intragastric administrated at identical voluminal normal saline. In each group, nine rats were chosen randomly to be killed at the 7, 14 and 21 d after operation for histological examination of kidney tissue.Renal tissues were examined by HE and Masson stain. The expression of NF-κB and PAI-1 were detected by immunohistochemical staining, and the expression of PAI-1-mRNA in renal tissue was determined by reverse transcription-polymerase chain reaction (RT-PCR). Result: The relative area of renal interstitial fibrosis of UUO model group at 7, 14 and 21 d was (13.03±0.66)%,(25.76±1.47)%,and (53.16±2.45)%respectively. And that of bicyclol-treated group was (9.63±0.58)%,(16.84±0.83)% and(33.59±1.61)% respectively. Compared with UUO model group, fibrotic area of bicyclol-treated group was decreased markedly(P<0.05, respectively). The protein expression of NF-κB of UUO model group at 7, 14 and 21 d was (11.73±0.42)%,(22.56±0.69)% and(36.27±1.14)% respectively, but that of bicyclol-treated group was (5.67±0.42)%,(10.79±0.37)% and(26.62±0.23)% respectively. The protein expression of PAI-1 of UUO model group at 7, 14 and 21 d was (7.29±0.23)%,(12.32±0.20)% and (18.36±0.19)% respectively, but that of bicyclol-treated group was (4.26±0.14)%,(7.69±0.13)% and (13.35±0.21)% respectively. The expression of PAI-1 mRNA of UUO model group at 7, 14 and 21 d was (1.17±0.10),(2.29±0.07) and (3.33±0.10) respectively, but that of bicyclol-treated group was (0.32±0.03),(1.18±0.05)and(2.06±0.40) respectively. Compared with UUO model group, the expression of NF-κB, PAI-1 protein and PAI-1 mRNA of bicyclol-treated group was decreased markedly(P<0.05, respectively). Conclusion: Bicyclol can alleviate the degree of renal interstitial fibrosis and protect renal function, down-regulates the expression of PAI-1 and NF-κB, thus blocking the renal fibrosis.关键词：renal interstitial fibrosis;bicyclol;NF-κB;PAI-1
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发布时间：2024-01-04 - 摘要：Objective: To investigate the absorption mechanism of palmatine chloride by using Caco-2 monolayer model. Method: A Caco-2 cell monolayer model was used to investigate the bi-directional transport of palmatine chloride. The effect of time, drug concentration, temperature, pH and inhibitors on the absorption of palmatine chloride was studied. Apparent permeability coefficient (Papp) of palmatine was calculated based on quantitation by HPLC. Result: Directional flux studies with palmatine chloride suggested transport with the permeability in the apical to basolateral direction higher than that in the basolateral to apical direction. Transport of palmatine chloride could be of saturation with time and concentration, and it was also effected by P-glycoprotein inhibitor, pH and temperature. Conclusion: The absorption of palmatine chloride in Caco-2 cell model was an active transportation mediated by transporter, which mainly located in the apical side of Caco-2 cell monolayer.关键词：palmatine chloride;Caco-2 cell model;active transport
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effects of integrative medicine for bronchial pneumonia in children. Method: Fifty cases of bronchial pneumonia were randomLy divided into control group and treatment group, all were given cephalothin sodium or penicillin and symptomatic treatment, in the same time the treatment group were used reduning injection. Result: The effective rate of the control group was 88%, the treatment group was 100% and no adverse reactions. The difference was statistically significant (P＜0.05). Conclusion: Integrative medicine for bronchial pneumonia in children have good dffect.关键词：bronchial pneumonia;integrative medicine;cephalothin sodium;reduning injection
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发布时间：2024-01-04 - 摘要：Guanxin Ⅱ decoction is a popular traditional Chinese medicine. In clinical,it has been widely used in treating coronary artery diseases,and angina pectoris,and so on. Pharmacokinetic studies of Guanxin Ⅱ decoction have been done.In this study, we have reviewed recent studies of Guanxin Ⅱ decoction, including its pharmacology, clinical and pharmacokinetics, in the context of the decoction.关键词：Guanxin Ⅱ decoction;pharmacology;clinical;pharmacokinetics
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发布时间：2024-01-04 - 摘要：With the development of social economy and the improving of living standards, the prevalence of hyperuricemia increased year by year. Hyperuricemia has been closely associated with metabolic syndrome and become a serious metabolic disease damaging human being,s health. In recent years, it has been paid more and more attention to Chinese medicine in the prevention and treatment of hyperuricemia, but also has made some achievement and progress in basic experimental study of hyperuricemia with Chinese medicine. This article reviews the progress on experimental study of hyperuricemia by traditional Chinese medicine in recent years.
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发布时间：2024-01-04 - 摘要：Coniferyl ferulate was widely present in various plants. In recent years, many studies were reported on it. However, more and more attention was paid to its excellent pharmacological activities discovered. In order to research it more systematically, the related reports during the past three decades years had been searched and summarized, including its physical and chemical properties, extraction and separation, content determination, stability and biological activities. Many studies showed that coniferyl ferulate is poorly unstable and easy to decompose,especially in the condition of strong light and high temperature, and so far the pressurized liquid extraction method was a better way to extract and high performance liquid was the method common used to content determination; Many of the strong pharmaceutical activities such as antimicrobial, anticancer, antioxidant, diastolic blood vessels were found,which provide information reference to quality standards and product development of chinese medicine containing Coniferyl ferulate.关键词：Coniferyl ferulate;ferulic acid;Ligusticum chuanxiong;Angelica sinensis;stability;biological activity
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发布时间：2024-01-04 - 摘要：To explore the effect of the climate, soil and terrain factors on the growth, distribution and quality of medicinal yinhua, this paper reviews relevant literatures in recent years, summarizes the correlation between medicinal yinhua and climate factors,soil factors,terrain factors of environment factors, analyses the environment factors on the influence of medicinal yinhua. The result indicates that, light is probably the most important influence on medicinal yinhua factors. The research provides references for the comprehensive development and utilization.关键词：medicinal yinhua;environment factors
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发布时间：2024-01-04 - 摘要：Objective: To analyse the contents, methods and findings in Chinese medicine dispensing granule (CMDG) related articles which was collected by Wangfang data,VIP information and CNKI in 2001-2010. Method: CMDG related articles in the three Chinese full-text database were retrieved and divided into nine categories: CMDG preparation process, chemical analysis (identification of the active ingredient, quality control, etc.), pharmacodynamics, toxicology, clinical safety evaluation, reporting, review and others, the number of these articles was counted at the same time. Result: The number of CMDG articles increased at first and then decreased, the peak appeared in 2007.The number of CMDG chemical research of CMDG is the most, followed by clinical applications and reports, then the pharmaceutical technology and pharmacodynamics studies, research of CMDG toxic and clinical safety is the least. Conclusion: Study of the CMDG tends to be more and more profound,and should pay more attention to the toxicology and clinical safety evaluation.关键词：chinese medicinal dispensing granule;literature research
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