最新刊期
卷 17 ， 期 6 ， 2011
- 摘要：Objective: To study the adaptability of hygroscopic kinetics curve model of traditional Chinese medicine extract powder. Method : SPSS14.0 statistical analysis software was employed in this study to evaluate the adaptability of the distribution curve of the moisture sorption of six extract powders of traditional Chinese medicine with the coefficient of determination (R2), the residues sum of squares (RSS) and AIC values as index. Result: The double exponential model was the best, first order kinetics model was the second, and the other four kinetics models were the worst. Conclusion: Double exponential model has better adaptability for the fitting of the moisture sorption process of traditional Chinese medicine extract powder.关键词：traditional Chinese medicine extract powder;moisture sorption;kinetics model;adaptability
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发布时间：2024-01-04 - 摘要：Objective: To study the optimum methods of extracting and refining ginsenosides from red ginseng. Method: The ability of extracting and refining ginsenosides was compared in different types of macroporous resin and different technological conditions with total amount of ginsenosides as index. Result: The HPD-400 macroporous resin was best for separating and purifying total saponins in the following technological conditions: the sample concentration was 1.7 g ·L-1, pH 4.68, the flow rate was 7.5 BV ·h-1, the eluting reagent was 80% ethanol (4 times the volume of column) and eluting velocity was 7.5 BV ·h-1. Conclution : The technological condition will provide bases for the preparation technique of Traditional Chinese Medicine containing ginsenosides.关键词：Macroreticular resin;Red ginseng;total ginsenosides;purification
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发布时间：2024-01-04 - 摘要：Objective: This paper describes the intrinsic viscosity difference of Gardenia extract treated with pectinase. Method: The intrinsic viscosity analysis was carried out on an Ubbelohde viscometer to determine the change after treated with different relatively density, amount of pectinase, time, temperature, etc. And the changes of geniposide, genipin-1-β-D-gentiobioside and deacetyl-asperulosidic acid methyl ester were also detremined respectively. Conclusion: Pectinase brought about the change of content of geniposide, genipin-1-β-D-gentiobioside and deacetyl-asperulosidic acid methyl ester, the maximum change rate of them was genipin-1-β-D-gentiobioside.关键词：pectinase;Gardenia jasminoides Ellis;intrinsic viscosity
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发布时间：2024-01-04 - 摘要：Objective: To prepare total Dracocephalum moldevica flavonoids osmotic pump tablets and study its factors influencing on drug release in vitro. Method: The UV spectrophotometer was used to determine the release of the osmotic pump tablets, and various factors affected the drug release were studied. Result: The type and amount of penetration enhancers, the amount of sodium bicarbonate, and the coating weight had significant effects on release in vitro. Conclusion: The preparation of osmotic pump tablet is reasonably and stable and the formulations can meet the requirement of the controlled release drug delivery.关键词：total Dracocephalum moldevica flavonoids;osmotic pump tablet;release
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发布时间：2024-01-04 - 摘要：Objective: To screen out the optimum technology for purification of total anthraquinones from Radix et Rhizoma Rhei. Method: Adsorption performance and desorption capacity were compared with different types of macroporous resin with the content of total anthraquinones as the target, so as to select the appropriate resin category. Thus, specific parameters of greatest sample volume, sample speed and elution solution etc were selected from resin purification technology by single-factor study. Result: The technology for AB-8 macroporous resin to purify total anthraquinones from Rheum could not only guarantee the elution rate of 82.96% of major effective indigent, but also decrease the solid content from 38.03% to 12.23% at the same time. Conclusion: AB-8 had a good effect on the adsorption of total anthraquinones from Rheum and has a good appliccation prospect.关键词：purification;macroporous resin;total anthraquinones;determination
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发布时间：2024-01-04 - 摘要：Objective: The method was established to determine the entrapment efficiency for the APPS liposome. Method: Anion exchange resin was used to separate the liposome and free APPS; UV-visible spectrophotometry was used to determine the content of APPS, and liposome encapsulation efficiency was calculated. Result: The method established was as follows: the anion-exchange resin was used with 1.0 mol ·L-1NaCl as eluent; the free APPS recovery was 99.21%, liposome recovery was 0.07% with the linear range of 11.66-58.29 mg·L-1(r=0.999 4). The intra- and inter-day precisions (RSD) could meet the requirements (within 2.0%); the average recovery was 99.28%, RSD was 0.66%. The encapsulation efficiency of APPS liposome was 70.16% on average. Conclusion: This method is simple and feasible, and can determine the entrapment efficiency for the APPS liposome.
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发布时间：2024-01-04 - 摘要：Objective: To study the optimized extraction process of berberine hydrochloride, one of the effective parts of Kuolougenwan. Method: An orthogonal experimental design L9(34) test was adopted in this study. And the content of berberine hydrochloride was determined to fit its quality standard, screening out the best method of extraction conditions. Result: The best extracting technical conditions were as follows: 10 times amount of water, extraction for 2 hour and extracted 2 times. Conclusion: The optimized process is simple, fast and feasible with good stability and repeatability.关键词：Kuolougenwan;orthogonal test;extraction;berberine hydrochloride
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction technology of Rhizome Cimicifugae. Method: The content of total saponins (measured by cimicifuga glycosides H-1) and total phenolic acids (the sum of ferulic acid, isoferulic acid and caffeic acid) was selected as the evaluation index for the first time. Orthogonal test was carried out to achieve the best extraction technology of Rhizome Cimicifugae by multiple guidelines grading methods. Result: The optimum extraction condition was that the medicinal materials were extracted 3 times with 6 times amout of 70% alcohol-0.1mol·L-1 HCl (95 ∶5) and for 1.5 hours per time. Conclusion: The optimum extraction condition was stable and feasible. It will provide a theoretical basis for regulating the extracting process of Rhizome Cimicifugae and lay the foundation to the further research and use of Rhizome Cimicifugae.关键词：Rhizome Cimicifugae;extraction technology;orthogonal test;total saponins;total phenolic acids
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction process of total flavonoids in Resina Draconis. Method: The extraction of total flavonoids was chosen as the assessment index. The extraction process was optimized with the orthogonal design using spectrophotometer to determine total flavonoids content at 508 nm wavelength. Result: The optimum ethnol extracting process conditions were as follows adding 8 fold of ethyl acetate, extracted two times, heating for 1 hour. Conclusion: The optimum extraction process of total flavonoids in Resina Draconis was reliable and efficient.关键词：Resina Draconis;total flavones;orthogonal test;extraction process
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发布时间：2024-01-04 - 摘要：Objective: To optimize the optional extraction method of Radix Linderae from Dangwu Dispersible Tablet. Method: Extracting method, extraction solvent and extraction times were selected to determine the factors and levels by single-factor test method with the content of linderane as index. Then content of linderane was used as index to evaluate the technologies based on orthogonal design, considering ointment rate at the same time, in which 4 factors considered were the concentration of ethanol, solvent consumption, extraction temperature and extracting time. Result: The optimum extraction processes was ultrasonic extraction technique, 50 ℃, adding 6 times amount of 90% ethanol, extracting for two times with 20 min each time. Conclusion: The method has the advantage of speediness, higher extraction rate etc. This process is stable and reliable.关键词：Radix Linderae;linderane;single-factor test;orthogonal experiment
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of S.baicalensis. Method: The optimization processing technology of S. baicalensis was investigated using orthogonal design with the yield of baicalin as the index to determine the processing of parameters. Result: The optimal condition for the processing technology of S. baicalensis was dosed in hot water 200 ℃ by steaming 50min. Conclusion: The optimized extractive technology is scientific and efficient.关键词：Scutellaria baicalensis;baicalin;processing technology;Orthogonal Design
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发布时间：2024-01-04 - 摘要：Objective: To optimize the technical conditions of ethanol percolation extraction for Citri Grandis Exocarpium Plaster. Method: The content of naringin extracted from Citri Grandis Exocarpium, which was regarded as the marker of detection, was determined by high performance liquid chromatography and was used to optimize the extraction processing conditions by L9(34) orthogonal test. Result: The optimum extraction condition was that the concentration of ethanol was 80%, the volume of solvent was 15 times of medicinal materials, the soaking time was 24 hours and the percolating speed was 5 mL ·min-1 ·kg-1. The average transferring rate of naringin in 3 batches of samples was 79.0%. Conclusion: The optimum extraction process is reasonable and the transferring rate of naringin is higher.关键词：orthogonal test;percolation extraction process;Citri Grandis Exocarpium;naringin;high performance liquid chromatography
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发布时间：2024-01-04 - 摘要：Objective: To establish the optimum preparation process and investigate its dissolution of Osthole droping pills. Method: The proportion of osthole to matrix, processing temperature, drops distance and drops speed on the basis of the preliminary experiments were investigated with the spherical degree, the weight coefficient of variation, dissolved casual time and appearance evaluation of the dropping pills as the scoring indexes, and ultraviolet measuring technology was used to study its dissolution. Result: The optimal conditions were that proportion of matrix to osthole was 6 ∶1, matrix PEG3000:PEG6000 was 6 ∶1, processing temperature was at 60 ℃, drops rate at 12 d ·min-1 and 20cm distance above liquid level. Conclusion: The preparation method is simple, convenient and can be controlled easily. It is also consistent with the items on pills preparation requirements in Ch.P (2005). The dissolution of products is good.关键词：osthole;droping pill;orthogonal test;technical study
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发布时间：2024-01-04 - 摘要：Objective: Research the method of ZTC1+1 natural clarifying agent for the removal of extraneous materials in Paeonia Lactiflora medicine. Method: Single factor design method was used for investigating ZTC1+1 natural clarifying agent of the relevant factors and the optimum combination of factors and alcohol precipitation methods were compared. Result: The effect of ZTC1+1 natural clarifying agent of impurity was better than alcohol precipitation. Conclusion: Natural clarifying agents with ZTC1+1 for aqueous extract of Chinese medicine is a better way to impurity. The method is convenient, economical and safe with the effect of imparity removal.关键词：Paeonia Lactiflora;ZTC1+1 natural clarifying agent;impurity
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for the Content determination of chlorogenic acid, paeoniflorin and baicalin inYinqiaochaigui decoction. Method :HPLC-based separation of the agents was performed on Kromasil Cl8 column (4.6 mm×250 mm,5 μm) at 25 ℃ with the mobile phase of methanol-0.1% Phosphoric acid water solution (gradient elution),flow rate of 1.0 mL ·min-1 and multiple detection wavelength of 327,230, 280 nm. Result :HPLC allowed simultaneous quantitative determination of the 3 components in Yinqiao Chaigui decoction, and they showed good linear relationships when their sample amount ranged 0.077 6-0.776 0 μg,0.076 1-0.761 0 μg and 0.264 8-2.648 μg,respectively,with correlation coefficients all beyond 0.999 9 and average recovery rates of 102.67%,97.85% and 101.86%, respectively. Conclusion :This method is simple and convenient in detecting the 3 components in Yinqiao Chaigui decoction, and the results are precise and reproducible.关键词：Yinqiao Chaigui decoction;chlorogenic acid;paeoniflorin;baicalin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop a reversed-phase HPLC method for the determination of swertiajaponin in the leaves of Lophatherum gracile. Method: Cosmosil 5 C18-ms-Ⅱ (4.6 mm×250 mm, 5 μm) column was adopted with the mobile phase of acetonitrile-0.05% acetic acid solution (14:86); the flow rate was 1 mL ·min-1, and the detection wavelength was set at 350 nm. Result: The calibration curve was linear in the range of 0.3~5.0 μg (r=1.000 0); the average recovery was 100.2 %. Conclusion : The content of swertiajaponin in the leaves of L. gracile from 8 different sources was determined for the first time. The developed method is simple, accurate with good reproducibility.关键词：Lophatherum gracile;swertiajaponin;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: The method of determining polysaccharides and their monosaccharides composition by CZE was established to provide a reference for discussing the effective substance of Polygonatum and Odoratum. Method: The degradation conditions of Polygonatum and Odoratum polysaccharides were optimized by orthogonal tests. The degradative monosaccharides, which were derived with 1-phenyl-3-methyl-5-pyrazolone (PMP), were separated and determined by CZE, and the results were compared with those which were determined by phenol-sulfuric acid method. Result: The Polygonatum and Odoratum monosaccharides all included xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid and galacturonic acid. However, the ratio was significant different. Conclusion : The analysis of Polygonatum and Odoratum polysaccharides and the composition of monosaccharide by CZE are accurate and reliable with satisfactory results. The similarities and differences of the effectiveness were evidenced by their monosaccharide composition in some degree, and the results suggested that their clinical application could not be confused.关键词：Polygonatum;Odoratum;polysaccharide;capillary zone electrophoresis;phenol sulfuric acid method
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发布时间：2024-01-04 - 摘要：Objective: Determination of α-terthienyl in Radix Echinopsis from different places by using RP-HPLC. Method: The separation was carried out at 25 ℃ on a Hypersil ODS2 C18 column (4.6 mm×250 mm, 5μm) with MeOH 0.1% acetate solution (85 ∶15) as mobile phase. The detective wavelength was at 352 nm with the flow rate of 1.0 mL ·min-1. Result: The α-terthienyl was linear within the concentration range of 0.4916-6.1450 μg (r=0.999 9). The average recovery was 98.83%. Conclusion: This method is simple and practical with strong specificity and good reproducibility.The content of α-terthienyl is up to the regulation of no less than 0.2% in Chinese Pharmacopoeia (Edition 2010) from different places.关键词：HPLC;Radix Echinopsis;α-terthienyl;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish the chromatographic fingerprints of Jinkui Shenqiwan by RP-HPLC. Method: A gradient mobile system was applied in the experiment. The fingerprints of different samples were compared with similarity evaluation software published by committee codex. Result: Sixteen peaks were identified as the characteristic fingerprints. The samples made by ourselves had good similarity, but there were great differences among different producers. Conclusion: This method shows high precision and good repeatability; it can be used as the method to assess the quanlity of Jin Kui Shenqiwan.关键词：Jinkui Shenqiwan;fingerprints;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To isolate and determine the chemical constituents of Ranunculus sceleratus. Method: The column chromatographic techniques were applied to isolate the constituents. Result: The spectroscopy methods were used of EI-MS and NMR to identify the structures of the separated compounds. Five compounds were isolated from R. sceleratus and their structures were elucidated. Conclusion: Compound 3-5 were isolated for the first time from this plant and 5 was isolated for the first time from the genus.关键词：Ranunculaceae;Ranunculus sceleratus;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of catechin in crude drugs from various habitats and prepared medical herbs of Cynomorium songaricum from different areas by HPLC. Method: The determination was carried on Tigerkin C18column, 0.04 mol ·L-1 citric acid-N,N-dimethylformamide-tetrahydrofuran (52 ∶5 ∶1) as mobile phase with a flow rate 1.0 mL ·min-1 and detection wavelength at 280 nm. Result: Among the crude drugs from 10 various habitats,the content of catechin in crude drugs from 9 various habitats is over 0.080% except from gannan which was 0.048%; Among the prepared medical herbs from 22 regions,the content of catechin in 5 various habitats is over 0.080%, the content in most prepared medical herbs was over 0.040%. Conclusion: The contents of catechin in crude drugs from various habitats and prepared medical herbs of Herbe cynomorii from different areas were different,the content of catechin in prepared medical herbs was lower than that in crude drugs.关键词：catechin;crude drugs and prepared medical herbs of Cynomorium songaricum Rupr.;content determination
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发布时间：2024-01-04 - 摘要：Objective: To establish the fingerprint of leaves of Adinandra nitida by RP-HPLC. Method: The chromatographic conditions were as follows: a Kromasil C18 column was used; the mobile phase was composed of acetonitrile-0.1% phosphoric acid with gradient elution; the flow rate was 1 mL ·min-1 and the UV absorbance detection was set at 254 nm. Result: Under the selected chromatographic conditions, 10 batches of HPLC fingerprint of A. nitida with good similarity were obtained, no less than 0.9. Conclusion: Quality of A. nitida can be controlled effectively by HPLC- UV fingerprint.关键词：Adinandra nitida;fingerprint;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of seven components such as paeoniflorin, ginsenoside R1, ginsenoside Rg1, forsythin, harpagoside, ginsenoside Rb, dehydrocostuslactone contained in Rupixiao granule. Method: HPLC was conducted on an Alltima C18 column (4.6 mm×250 mm, 5 μm) at 30 ℃; the mobile phase was consisted of acetonitrile (A)-water (B) with gradient elution at the flow rate of 1.0 mL ·min-1 detected at 203 nm. Result: The linear range (mg ·L-1) of paeoniflorin, ginsenoside R1, ginsenoside Rg1, forsythin, harpagoside, ginsenoside Rb, dehydrocostuslactone was 3.906 25-125 (r=0.999), 9.375-300 (r=0.999), 37.5-1 200 (r=1.000), 2.1875-70 (r=0.999), 3.75-120 (r=0.999), 25-800 (r=0.999) and 0.625-20 μg(r=1.000) respectively. The average recoveries were 97.95% (RSD 0.71%), 99.05% (RSD 1.11%), 100.70% (RSD 2.46%), 102.74% (RSD 0.35%), 103.06% (RSD 0.55%), 98.25% (RSD 1.68%) and 100.16% (RSD 1.82%) respectively. Conclusion: The developed method was convenient, reliable and accurate for the quality control of Rupixiao preparation.关键词：Rupixiao granule;paeoniflorin;Ginsenoside Rg1;harpagoside;Forsythin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: Research of the compatibility of Corydalis Rhizoma with Carthamus Flos on the dynamic change of tetrahydropalmatine for regulation of Chinese crude drug compatibility and discussing the rationality. Method: The compatibility of Corydalis Rhizoma with Carthamus Flos after the decoction of two kinds of effective ingredients was studied to determine the dynamic change of tetrahydropalmatine. Result: The tetrahydropalmatine content decreased at 75 minutes after compatibility of Corydalis Rhizoma with Carthamus Flos. Conclusion: Compatibility of fumaric with Carthamus Flos can reduce tetrahydropalmatine content, and the results can provide theory basis for compatibility test.关键词：TLCS;Corydalis Rhizoma;Carthamus;compatibility;content determination
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发布时间：2024-01-04 - 摘要：Objective: To study on the extraction and assaying of Huidouba polysaccharides, to provide new clues for drug treatment in clinical diabetes, and to develop and research new materials for treating diabetes mellitus, especially those from natural resources that is able to treat diabetes mellitus and no obvious side effects, which is of great significance. Method: The method of decocting with water and depositing with alcohol was adopted to extract polysaccharides from Huidouba. And after phenol-sulfuric acid reaction, the method of absorption spectrometry was used to determine the polysaccharides content of Huidouba. Result: The standard curve was linear in the range of 15-50 mg ·L-1 with correlation coefficient of r=0.999 3.The linear regression equation was A=0.015 2C+0.019 2.The average content of total sugars of Huidouba polysaccharides was 99.92% calculated using the conversion factor f=3.347 3. Conclusion: The method of phenol-sulfuric acid reaction used in determining the content of polysaccharides is simple in operation with high sensitivity, good stability and reproducibility, which can be used for quantity control of the polysaccharides content of Huidouba.关键词：Huidouba;polysaccharides;extraction;phenol-sulfuric acid method;assaying
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发布时间：2024-01-04 - 摘要：Objective: To analyze the changing regularity of the toxicity of the Mahuang Fuzi Gancaotang through different compatibility methods, and to determine the main factor for reducing its toxicity. Method : Different compatibility methods were used to allocate Ephedre sinica , Aconitum carmichaeli and Glycyrrhiza uralensis . The total alkaloids in all samples were isolated by extraction methods and by different pH value solutions. And ultraviolet spectrometer was used to analyze the content of total alkaloid and ester alkaloid in all compatibility samples. Result : The content of ester alkaloid in compatibility samples was decreased from 46% to 10%, while the content of non-ester alkaloid in compatibility samples was increased from 54% to 89%. Conclusion : E. sinica is similar to G.uralensis in decreasing the toxicity of A.carmichaeli largely and retaining its activity. And the effect of decreasing toxicity is better than other compatibility methods when three kinds of medicines were decocted together at the same time. So, it has important significance to reveal the mechanism of compatibility.关键词：Mahuang Fuzi Gancaotang;compatibility;alkaloid;toxicity
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发布时间：2024-01-04 - 摘要：Objective: To search the volatile constituents from aerial parts and roots of Indigofera stachyodes. Method: The volatiles were analyzed by head-space solid micro-extraction, coupled with GC-MS and Kovats indices for the first time. A quantitative analysis in percent was performed by peak area normalization measurements. Result: As a result, 35 compounds were identified from the aerial parts, and 21 from roots, 85.66% and 93.74% of the total essential constituents respectively. 12 compounds were shared in both samples. Conclusion: It was different between the aerial part and roots of I. staclyodes.关键词：Indigofera stachyodes;volatile constituents;SPME;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents in the leaves of Sophora japonica. Method: The constituents were isolated by column chromatography and their structures were elucidated by physico-chemical properties and spectroscopic analysis. Result: Fifteen compounds were isolated and identified as quercetin ( 1 ), kaempferol ( 2 ), isorhamnetin ( 3 ), genistein ( 4 ), prunetin ( 5 ), daidzein ( 6 ), calycosin ( 7 ), pyrocatechol ( 8 ), protocatechuric acid ( 9 ), eicosyl behenate ( 10 ), eicosanol ( 11 ), behenic acid ( 12 ),β-sitosterol ( 13 ), stigmasterol ( 14 ) and daucosterol ( 15 ). Conclusion: All of the constituents are obtained in the leaves of S. japonica; among them, compounds 7-10, 12 and 14 are reported in this species for the first time. The results are helpful for the comprehensive utilization of pagoda tree resources.关键词：Sophora japonica;leaves;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Melia azedarach. Method: The chemical constituents were isolated and purified by column chromatography, and their structures were identified by spectroscopic analysis. Result: Seven compounds were isolated and identified as isosclerone (Ⅰ), fraxinellone (Ⅱ), kulinone (Ⅲ), kulactone (Ⅳ), dubione B (Ⅴ), kulonic acid (Ⅵ), β-sitosterol (Ⅶ), succinic acid (Ⅷ) and 5-(hydroxymethyl)-2-furaldehyde (Ⅸ). Conclusion: CompoundsⅠ,Ⅷ and Ⅸ were isolated from this plant for the first time.关键词：Melia azedarach;chemical constituents;structural identification
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发布时间：2024-01-04 - 摘要：Objective: To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Hibiscus Mutabilis. Method: HPLC method was set up by Waters Xbridge C18column (4.6 mm×250 mm, 5 μm) with gradient elution; the mobile phase was comprised of acetonitrile-1% acetic acid solution, the UV detection wavelength was at 370 nm with a flow rate of 1.0 mL ·min-1, and column temperature was at 25 ℃. Result: There were 10 common peaks in the fingerprints of H.mutabilis based on 10 habitats. Sum of peak areas was larger than 90% of total area. As compared with standard sample, rutin, hyperin and quercitrin were checked out. Conclusion: The method is reliable, accurate and can be used as a quality control method for H.mutabilis.关键词：Hibiscus m;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish the method of the dissolubility of puerarin in Yiyin Xiaoke Pills. Method: Basket-stirring method was used for the determination of in vitro dissolution and HPLC method was applied to determine the accumulated dissolution rate of puerarin at the different time. Result: The calibration curve was linear in the range of 88.4-2 210 μg, (r=0.999 9). The accumulated dissolution rate of puerarin in Yiyin Xiaoke pills reached 88.47% in 10min, while the accumulated dissolution rate of puerarin in capsule was only 0.25%. Conclusion: Yiyin Xiaoke pills can improve the dissolution rate of poorly soluble drugs of the kind of puerarin.关键词：Yiyin Xiaoke pills;puerarin;in vitro dissolution
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of total flavonoids in the leaves of Apocynum venetum by HPLC. Method: The total quercetrin and kaempferol content in the flavonoids was determined by HPLC after hydrolysis. The assay was performed on Shim-pack CLC-ODS column; methanol-0.4 % phosphoric acid (45 ∶40) was used as mobile phase; the flow rate was 1.0 mL ·min-1, detection wavelength was at 360 nm, temperature of column was at 30 ℃. Result: Quercetrin was linear in the range of 5.52-55.2 mg ·L-1 (r=0.999 9, n=6); the average recovery and relative standard deviation were 98.76% and 1.01% (n=6) respectively. Kaempferol was linear in the range of 3.505-35.05 g ·L-1 (r=0.999 9,n=6); the average recovery and relative standard deviation were 98.62% and 1.14% (n=6) respectively. The content of total flavonoids in three samples was 17.08, 16.54 and 16.81 mg ·g-1 respectively. Conclusion: This method was accurate and reliable. It can be used for the determination of the flavonoids in the leaves of A. venetum. It will provide the basis for the quality standards of Luobuma leaves and further study.关键词：Apocynum venetum leaves;total flavonoids;HPLC;determination;hydrolysis
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发布时间：2024-01-04 - 摘要：Objective: To establish an analysis method for the determination of the content of polysaccharides from Prunus mume. Method: Using Phenol-sulfuric Method to determination of the content of polysaccharides for P.mume. Result: The absorbance was determined at 488 nm. The linear curves was fine between the concentration rang of 0.02-0.2 g ·L-1 (r=0.999 8), the average recovery was 100.38%, the RSD was 0.892%.The content of polysaccharide in P.mume was 2.51%. Conclusion: Phenol-sulfuric method is a simple operation process with accurate result, laying a foundation for further study on polysaccharides in P.mume.关键词：Prunus mume;polysaccharides;phenol-sulfuric method
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of boron and mercury in Bingpengsan. Method: The samples were digested by microwave. Boron and mercury in Bingpengsan were directly analyzed by ICP-MS. Bi and Be were selected as the internal standard, and the instrument parameters were adjusted to the optimum working conditions. Serial standard solutions were prepared. The reagent blank, standard series and the sample solutions were added to equipment and detected. The accuracy of the method was validated by recovery rate. Result: Mercury was linear in the range of 0-60 μg ·L-1; the average recovery was 99.7%. Boron was linear in the range of 0-80 μg ·L-1; the average recovery was 99.4%. Conclusion: This method is convenient, quick, accurate and highly sensitive. The method can be used for the quality control of Bingpengsan.关键词：ICP-MS;microwave digestion;Bingpengsan;mercury;boron
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method for bilirubin in mongolia drug sanchen pill. Method: The analysis was carried out on a Kromasil C18(4.6 mm×200 mm, 5 μm)and mobile phase was methanol-acetonitrile-1%glacial acetic acid(88 ∶10 ∶2)with a flow rate of 1 mL ·min-1 detection wavelenth was at 453 nm. Result: Bilirubin was linar in hte range of 0.0118-0.472 μg (r=0.999),RSD 1.86%. Conclusion: The method is qiuck simple and reproducible for the determination of bilirubin in mongolia drug sanchen pill.关键词：mongolia drug;sanchen pill;bilirubin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the compatible stability of Shuanghuanglian powder injection and 9 common injection. Method : The samples of complexes with the sodium chloride solution as a solvent were collected at room temperature to study the changes of appearanc, pH and to determine content by using HPLC method. Result : White precipitate produced and then content decreased after mixing Shuanghuanglian powder injection with cefuroxime sodium injection. deepened color, pH slightly increased and content decreased after mixing Shuanghuanglian powder with sodium bicarbonate injection and hydrochloric acid injection. Conclusion: Shuanghuanglian powder could not combine with cefuroxime sodium powder for injection or sodium bicarbonate injection, but can combine with other drugs.关键词：Shuanghuanglian powder injection;compatible;stability;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint of the pieces of Radix Alangii Preparata. Method: Using the high performance liquid chromatography,the chromatographic column was Alltech Apollo C18 (4.6 mm × 250 mm, 5 μm) and acetonitrile-buffer (buffer: 0.2 g heptane sulfonate, 3.5 g potassium dihydrogen phosphate volume to 1 000 mL)was selected as mobile phase gradient elution were adopeted. Result :Established HPLC fingerprint of R Alangii Preparata, and the results of methodological studymet the technical requirements for fingerprinting. Conclusion: The method is stable, accurate, and reliable, to provide a scientific basis of quality control standard for the R.Alangii Preparata.关键词：Radix Alangii Preparata;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: Using in vitro everted gut scas to investigate the intestinal absorption of the extracts from Corydalis yanhusuo at different intestinal section and different concentration. Method: Corydaline (CDL) and Tetrahydropalmatine (THP) which are representative compositions of the extracts from Corydalis yanhusuo in everted gut scas were detected by HPLC, and the absorption parameter to describe the character of absorption was calculated. Result: The absorption of CDL and THP was linearity in different intestine and different dose, and the square of coefficient correlation exceed 0.95, which consistent with zero order rate process. The Ka of CDL and THP increased along with the raised dosage of the extracts from Corydalis yanhusuo (P<0.05), indicated the passive absorption. The absorption of CDL or THP in duodenum, jejunum, ileum was quick, but the absorption of CDL or THP in colon absorption was slow. And the absorption of THP was quicker than that of CDL. Conclusion: For different dosage of extracts from Corydalis yanhusuo, the absorption of CDL and THP conforms to the zero order rate process at different sections of intestine, with a pattern of passive absorption.关键词：extracts Corydalis yanhusuo;everted gut sacs;Corydaline;tetrahydropalmatine;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study anxiolytic activity of valeportriate isolated from the root of Valeriana jatamansi. Method: Sixty Sprague-Dawley rats were randomly divided into 5 groups for daily intragastric administration of drugs: valeportriate high-dose (20 mg ·kg-1), valeportriate medium-dose (10 mg ·kg-1), valeportriate low-dose (5 mg ·kg-1), the positive drug group and normal control group, treated ig for 10 days. Adopting the international common-used anxiety animal model with the elevated plus-maze test (EPM),we observed the effect of anti-anxiety of valeportiate from the angle of behavior. Result: The results of EPM showed that DZP and valeportriate medium-dose group obviously increased the percentages of open arm time (14.07%, 38.09%, 37.42%, P<0.01) and entry (16.98%, 30.10%, 37.19%, P<0.05 or P<0.01) comparing with control group. Conclusion: Valeportriate has obvious anti-anxiety activity.关键词：anxiety disorders;valeportriate;elevated plus maze
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发布时间：2024-01-04 - 摘要：Objective: To compare the effects of Typhae Pollen before and after being carbonized on the hemorheological parameters and clotting time in acute blood-stasis model of rats. Method: The SD rats were divided into control, model, Yunnan White Drug-power 0.27 g ·kg-1, water extract solution of Typhae Pollen 1.2 g ·kg-1, carbo water extract solution of Typhae Pollen 1.2 g ·kg-1, carbo power of Typhae Pollen 1.2 g ·kg-1. Except the control group, blood stasis models were established in the rest groups. The hemorheological parameters, the prothrombinase time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) were detected in these groups. Result: Typhae Pollen and carbo of Typhae Pollen could decrease the ηb (the viscosity of whole blood) at high shear rate, ESR(erythrocyte sedimentation rate), K of ESR and rigidity of erythrocyte, which increased in model rats. Carbo of Typhae Pollen could shorten PT. Typhae Pollen and carbo of Typhae Pollen could shorten APTT, decrease FIB, and the potency of Typhae Pollen was stronger than the carbo. Conclusion: TyPhaePollen and carbo of Typhae Pollen showed the effect of invigorating blood and hemostasis by ameliotating the abnormal hemorheological parameters, shorting blood coagulation time, decreasing FIB. The effect of Typhae Pollen carbo on blood clotting was stronger than Typhae Pollen, while the effect of Typhae Pollen on reducing FIB was stronger than the carbo.关键词：Typhae Pollen;carbo of Typhae Pollen;hemorheology;hemostasis
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-stress and analgesic activities of water extraction of K-CoxB-JN, a compound Chinese medicine for treatment of myocarditis. Method: Water extraction of K-CoxB-JN was consecutively given to healthy Kunming mice with high (9.6 g ·kg-1), medium (4.8 g ·kg-1) and low dose (2.4 g ·kg-1) ig. At the same time, control group and positive group were set up. Analgesic activity was evaluated in mice model of pain induced by thermal stimulus and acetic acid. Meanwhile, anti-stress activity was evaluated by swimming and anti-hypoxia experiments. Result: Water extraction of K-CoxB-JN could enhance anti-stress capability of anti-fatigue and anti-hypoxia. The number of writhing induced by acetic acid was significantly reduced and the time of licking rear feet induced by hot plate stimulation was prolonged. Conclusion: Water extraction of K-CoxB-JN has good effects of anti-stress and analgesic capability.关键词：Water extraction of K-CoxB-JN;analgesic capability;anti-stress capability;anti-hypoxia
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Xiaoyaosan on changes of behavior and plasma monoamine neurotransmitters on chronic unpredictable mild stress (CUMS) model in rats. Method: The stimulus on CUMS model lasted 3 weeks. At the beginning of stimulus,the rats were treated with the decoction of Xiaoyaosan or two positive drugs (fluoxetine and cultural venlafaxine) for 4 weeks. During the experiment, body weight, the percentage of sucrose preference and data of open-field test (including ambulation numbers, rearing numbers, immobility time) were detected, which was to evaluate the anti-depression action of Xiaoyaosan. In the 4th week, all rats were decapitated after the last treatment, then blood was immediately isolated for detecting the concentration of NA and 5-HT by HPLC - fluorescence detection. Result: After 3 weeks of CUMS procedure, body weight, number of activities and percentage of sucrose preference of the rats in model group decreased significantly compare with control group (P<0.01 and P<0.05, respectively). Continually administrated for 4 weeks, the body weight, activity times and percentage of sucrose preference in the model group treated with Xiaoyaosan (92.5 g ·kg-1and 46.3 g ·kg-1) increased significantly (P<0.01 and P<0.05, respectively) compared with model group. Continuous treatment of 92.5,46.3 g ·kg-1and 23.1 g ·kg-1 Xiaoyaosan for 4 weeks could increase the concentration of NA in plasma (P<0.05). Conclusions: It suggested that Xiaoyaosan had the antidepression effect and the anti-depression effect was possibly related to increasing the concentration of NA in plasma.关键词：Xiaoyaosan;chronic unpredictable mild stress;anti-depression;plasma;neurotransmitter
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Siwu Decoction and its drug pairs on proliferation of rat ovary granulosa cell, and to probe the effect of combination of drug pairs. Method: Siwu Decoction and its drug pairs were added into the cultured rat ovary granulosa cells in vitro at the concentration of 2,20,200 mg ·L-1, and proliferation of the cells was measured by MTT. Result: Under the treatment of Siwu Decoction, the proliferation of granulosa cells was significant promoted by 36.73% in compare with the control group. The same effect was also found after treating with 5 drug pairs, and the order of promoting proliferation effects was: Angelicae Sinensis Radix-Chuanxiong Rhizoma (66.86%)>Angelicae Sinensis Radix-Paeoniae Radix Alba(58.52%)>Paeoniae Radix Alba-Chuanxiong Rhizoma(47.03%)>Paeoniae Radix Alba-Rehmanniae Radix Praeparata(41.43%)>Angelicae Sinensis Radix-Rehmanniae Radix Praeparata(23.67%). Water preparation (1 ∶2), ethanol preparation (1 ∶1) and ethanol-water preparation (1 ∶1.5) showed the strongest activity in each group extracted by different method. Conclusion: Siwu Decoction and its drug pairs showed significant promotion effects on rat ovary granulosa cell proliferation. Different pairing ratios of Angelicae Sinensis Radix to Chuanxiong Rhizoma and different extraction procedure contribute to different effects on the proliferation of rat ovary granulosa cell in vitro.关键词：SIWU decoction;drug pair;ovary granulosa cell;proliferation
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-apoptotic mechanism of tanshinoneⅡA and the function of prohibitin (PHB) on myocardial cell apoptosis induced by hydrogen peroxide(H2O2). Method: there were six groups of myocardial cells in the study: normal control group, oxidative stress group, tanshinoneⅡA group, siRNA group, siRNA+oxidative stress group, siRNA control group. Primary cultured neonate rat myocardial cells were cultured in medium with 200 μmol ·L-1 hydrogen peroxide, and the medium was supplemented with tanshinoneⅡA (1×10-4,5×10-5,1×10-5 mol ·L-1) in advance for 24 hours. PHB in myocardial cells was knocked down by small interfering RNA (siRNA) interference, and the expression level of PHB was determined by western blotting analysis. Flow cytometric analysis was used to detect apoptosis rate, i and mitochondrial membrane potential(MMP). Result: H2O2-mediated cell apoptosis resulted in activation of PHB, increasing of i, and decreasing of mitochondrial membrane potential. Tanshinone IIA profoundly prohibited myocardial cells apoptosis induced by hydrogen peroxide, and decreased i, and increased mitochondrial membrane potential in a dose dependent manner. Specific silence of PHB by siRNA down-regulated the expression level of PHB and increased of apoptosis rate and i, and decreased mitochondrial membrane potential. Conclusion: The results demonstrated that the activation of PHB is compensatory increased in myocardial cells against oxidative stress. tanshinone ⅡA could reduce the expression of PHB by attenuate the injury induced by oxidative stress.
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发布时间：2024-01-04 - 摘要：Objective: This study was designed to screen different combination of astragalus active ingredients for blood sugar regulation in diabetes rats, and to find the influences on blood glucose, serum insulin and adiponectin. Method: Diabetes rats were induced by STZ (52 mg ·kg-1, peritioneal injection ), some of which were administered by different combination of astragalus flavonoids, polysaccharides, and astragalosides for 30 days, the control group was parallelly established. After the treatment, detection of blood glucose and ELISA assay of serum insulin and adiponectin were carried out. Result: Body weight of diabetes rats was dropped, the blood glucose of diabetes was ascended,serum insulin and adiponectin descended(P<0.05).The blood glucose descended in astragalus flavonoid+astragalosides group (body weight gained for the group); astragalus group, astragalus flavonoid+polysaccharides group, astragalus flavonoid+astragalosides group and astragalus flavonoid+polysaccharides+astragalosides group. The best group was astragalus group for ascending serum insulin(P<0.05). The treatment groups showed regulative effect on serum adiponectin, the effect of astragalus group, astragalus flavonoid+astragalosides group and astragalus flavonoid+polysaccharides+astragalosides group were obvious(P<0.05). Conclusion: Astragalus and different combination of its active ingredients could influence the content of blood glucose, serum insulin and adiponectin in diabetes rats, the best result came from astragalus flavonoid+astragalosides group.关键词：active ingredients of astragalus;compatibility;blood glucose;insulin;adiponectin
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发布时间：2024-01-04 - 摘要：Objective: To optimize anti-coxsackie B complex capsule for treating myocarditis induced by coxsackie B virus with the method of orthogonal test. Method: The optimized prescription of anti-coxsackie B complex capsule was selected according to the survival time of mice after oxygen deficiency with the method of orthogonal t value design. The optimized prescription was verified by anti-stress reaction experiments including tolerance to oxygen deficiency after myocardial ischemia and weight loading swimming. Result: Four of the seven components in the original prescription were selected preferentially to compose the optimized prescription, including Panax quinquefolius, Astragalus mongholicus, Radix Ophiopogonis and Chinese magnoliavine fruit. Both the original prescription and the optimized prescription could remarkably prolong the survival time of oxygen deficient mice and the swimming time of weight loading mice, however, there was no significant difference between the two prescriptions. Conclusion: The optimized prescription had similar effects on elevating the ability of anti-stress action in mice as the original one with less dosage.关键词：anti-coxsackie B complex capsule;optimization;anti-stress action;viral myocarditis
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发布时间：2024-01-04 - 摘要：Objective: To study the vascular smooth muscle relaxation effect and explicit the material base of Gastrodia elata Blume. Method: Tension recording for rat isolated aortic artery was used to study the effect of vascular smooth muscle relaxation. Column chromatography and mass spectrography and nuclear magnetic resonance spectrometry were used to isolate and identify the active compounds of G.elata Blume. Result: G.elata Blume. could significantly inhibit the smooth muscle constriction of isolated aortic artery induced by KCl. Five esterified phenolic compounds(Ⅰ-Ⅴ) were isolated from G.elata Blume, such as p-hydroxybenz aldehyde(Ⅰ); p-hydroxybenzyl methylether(Ⅱ); p-hydroxybenzyl alcohol(Ⅲ); 4,4'-dihydroxydiphenyl methane(Ⅳ); 4,4'-dihydroxydibenzyl ether(Ⅴ). The results showed that the vascular smooth muscle relaxation effects were the result of the role of five compounds. Conclusion: The five esterified phenolic compounds(Ⅰ-Ⅴ) from G.elata Blume. play a combined role for vascular smooth muscle relaxation.关键词：Gastrodia elata Blume;vascular smooth muscle;active compounds;screening
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发布时间：2024-01-04 - 摘要：Objective: To investigate possible mechanisms of the intervention effects of prepared Rhei Radix et Rhizoma with wine on serum lipid, nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in rabbit models of atherosclerosis (AS). Method: Thirty-two healthy male New-Zealand rabbits were randomly divided into four groups (n=8): Normal control group(N),AS model group(M), low dose(L)and high dose(H)prepared Rhei Radix et Rhizoma with wine groups. After 12-week treatment,serum lipid was detected with automatic analysis device. The expressions of serum NO was detected with nitrate reductase method,and protein of iNOS was observed by immunohistochemistry method,iNOS mRNA in aortic tissue were detected by RT-PCR method. Result: At the end of the 12th week, compared with AS model group,levels of serum TC,LDL,and NO in L and H group were significantly decreased (P<0.01or P<0.05), however the level of serum HDL were increased obviously (P<0.01). The serum levels of TG in high dose group were significantly lower than that in AS model group but no significant difference between the low dose and model group. Conclusion: Prepared Rhei Radix et Rhizoma with wine can reduce the expression of iNOS gene and protein in aorta vessels of experimental atherosclerotic rabbits, reduce the production of NO and levels of serum lipids. and can inhibit the progression of atherosclerosis.关键词：prepared Rhei Radix et Rhizoma with wine;atherosclerosis;serum lipid;nitric oxide;inducible nitric oxide synthase
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发布时间：2024-01-04 - 摘要：Objective: To reveal the mechanism of the protection of Dusuqing Granule through the levels of the inflammatory factor expression in multiple organ injury caused by Klebsiella pneumonia. Method : Fifty-five male Sprague Dawley (SD) aged rats were randomlLy divided into control group (CG), model group (MG), Dusuqing group (DG) and Lomefloxacin group (LG). There were 25 rats in the model group and 10 in each of the other three groups. The models of multiple organ injury caused by pneumonia were made by injection of Klebsiella pneumoniae through the tracheal intubation. The expressions of inflammatory factors like tumor necrosis factor-α(TNF-α), interleukin-1(IL-1) and interleukin-6(IL-6) in the lungs, the theart, the kidney and the small intestine were measured. Result: The level of expressions of TNF-α, IL-1 and IL-6 in the lungs, the hear, the kedney and the small intestine in MG were significantly higher than those in CG (P<0.01 or P<0.05). The neutrophil rates and the counts of WBC in peripheral blood increased dramatictly in MG (P<0.01). The pathological changes under light microscope were obvious in the organs in MG (P<0.01 or P<0.05). Compared with MG, the levels of expressions of TNF-α, IL-1, and IL-6 in the lungs, the heart, the kidney and the small intestine in DG decreased significantly (P<0.01 or P<0.05). The neutrophil rates and the counts of WBC in peripheral blood dropped dramaticly in DG (P<0.01). The pathological injuries were light obviously in the organs in DG (P<0.01 or P<0.05). Conclusion: Low the levels of inflammatory factor expression, such as TNF-α, IL-1 and IL-6, so as to reduce organ damage, which might be one of the mechanisms of Dusuqing Granule’s protection in multiple organ injury.关键词：Dusuqing Granule;pneumonia;multiple organ injury;inflammatory factors
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Tongluo Shenggu Capsules on the osteoporosis induced by stress absence. Method: Fourty male SD rats were randomly divided into four groups: normal control, model control, high and low dose drug groups. Besides the normal control group, all animals in other three group were subjected to tail suspension test for 21 days, the animals in drug groups were administered Tongluo Shenggu Capsule. The bone metabolism markers and biomechanical parameters were determined. Reusult: Compared with the nomal control group, the alkaline phosphatase (ALP) and the concentration of calcium in serum of model group was decreased significantly, the area and thickness of bone tranecula, the amount of osteoblast were decreased obviously, compared to the model control, Tongluo Chenggu capsules could increase ALP, the area and thickness of bone tranecula and the osteoblast amount. Conclusion: Tongluo Shenggu Capsules could enhance the growth of bone and could be used to treat osteoporotic induced by stress absence.关键词：stress absence;osteoporotic;Tongluo Chenggu Capsules
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发布时间：2024-01-04 - 摘要：Objective: To investigate the bacteriostatic action of mangoferin monosodium salt. Method: Bacteriostasis in vivo and in vitro experiments for Diplococcus pennmoniae,Klebsiella pneumoniae,Staphylococcus aureus,Haemophilus influenzae was examined respectively to observe the antibiosis effect of mangoferin monosodium salt. Result: Mangoferin monosodium salt showed some extents of antibiosis effect to D.pennmoniae,K.pneumoniae,S.aureus,H.influenzae for in vitro experiment.The minimal inhibitory concentration (MIC) were 10.0,5.0,10.0,2.5 g ·L-1,respectively.Mangoferin monosodium salt also showed significant in vivo protection effect in infected mouse. Conclusion: Mangoferin monosodium salt has good bacteriostatic action.关键词：mangoferin monosodium salt;bacteriostati action;Diplococcus pennmoniae;Klebsiella pneumoniae;staphylococcus aureus;Haemophilus influenzae
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Shenxiong Huayu Capsule on the apoptosis and the expression of Bcl-2 and Bax protein in CA1 Area of hippocampus in vascular dementia rats. Method: The vascular dementia model of rat was established by repeated close and open of the common carotid arteries at both sides and intraperitoneal injection of sodium nitroprusside (SNP). The learning and memory abilities of rats were detected by Morris water maze. The cell apoptosis was detected by TUNEL staining and the expression of Bcl-2 and Bax protein were detected by immunohistochemical SABC staining. Result: ① The number of cell apoptosis in the CA1 area of hippocampus was increased in the VD model group compared with that in the sham-operation group and blank control group (P<0.01). The number of cell apoptosis in the CA1 area of hippocampus was decreased in Shenxiong Huayu Capsule intervention group than in the VD model group (P<0.01). ②The expression of Bcl-2, Bax in the CA1 area of hippocampus was increased in the VD model group compared with that in the sham-operation group and blank control group (all P<0.01), Bcl-2/Bax was decreased (P<0.01). The expression of Bcl-2 in the CA1 area of hippocampus was increased in Shenxiong Huayu Capsule intervention group compared with that in the VD model group (P<0.01), Bax was decreased(P<0.01) and Bcl-2/Bax was increased (P<0.01). Conclusion: Shenxiong Huayu Capsule can inhibit apoptosis by up-regulation of expression of Bcl-2 protein and down-regulation of Bax protein in CA1 area of hippocampus in vascular dementia rats and improve the learning and memory abilities of for the vascular dementia rats.关键词：vascular dementia;apoptosis;Bcl-2;Bax;Shenxiong Huayu Capsule
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Qishen Yiqi Dropping Pills on ventricular remodeling and function after myocardial infarction model in rats. Method: The myocardial infarction model of SD rat was established by logating the anterior descending coronary artery and then the rats were divided into myocardial infarction control group and medicine group (n=16 in each froup). Meanwhile, a sham-operation group was build including 16 rats. The rats in medicine group were intragastrically given Qishen Yiqi Dropping Pill at the dose of 38 mg ·kg-1 ·d-1. The rats in the control group and the sham-operation group were intragastrically given at the same dosage of normal saline. Eight weeks later,left ventricular end-diastolic pressure,left ventricular peak pressure, the maximum change rate of left ventricular pressure rise and fall, left ventricular mass and left ventricular mass index,serum and plasma angiotensinⅡ, tumor necrosis factor α,endothelin-1,brain natriuretic peptide, etc. were tested. Result:Eight weeks later, left ventricular end-diastolic pressure of the control group was higher than that of sham-operation group(P<0.05);left intraventricular systolic pressure,the maximum change rate of left ventricular pressure rise and fall were lower than those of the sham-operation group(P<0.05);serum ICAM-1 and plasma AngiotensinⅡ, tumor necrosis factor α, endothelin-1 and brain natriuretic peptide etc were higher than those of sham-operation group(P<0.05). After drugs intervention,left ventricular mass and left ventricular mass index,left ventricular end-diastolic pressure,serum angiotensinⅡ,tumor necrosis factor α,endothelin-1and brain natriuretic peptide of the rats in the medicine group were lower than those of the control group; left intraventricular systolic pressure, the maximum change rate of left ventricular pressure rise and fall were higher than those of the control group(P<0.05). Conclusion: Qishen Yiqi Dropping Pills can improve the ventricular remodeling and function for the myocardial infarction model in rats.关键词：Qishen Yiqi dropping pills;myocardial infarction;ventricular remodeling;heart function;mechanism
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发布时间：2024-01-04 - 摘要：Objective: To explore the in vitro effect of the toxin of Selenocosmia huwena on inhibiting the growth and inducing the death of cells of neurogliocytoma, and activating caspase-3. Method: MTT was applied to obtain the growth curves of cell affected by different concentrations of toxin of S.huwena for 24 h and 48 h. AnnexinV-FITC was used to test the death rate. Absorption spectrometry was adopted to measure the relative activity of caspase-3. Result: The toxin of S.huwena evidently inhibited the growth of U251, and it could induce the death of cells neurogliocytoma with concentration-dependent manner. With cells affected by different concentrations of toxin for 24 h, we found that the activity of caspase-3 reached the maximum at 48 h with concentration-dependent manner. Conclusion: The toxin of S.huwena evidently inhibits the growth of U251 and induces the death of U251, and the process include the activation of caspase-3.关键词：toxin of Selenocosmia huwena;gliocytoma U251;cell apoptosis;caspase-3
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Polysaccharides of Banana Peel on proliferation, colony formation and expression of nuclear factor- kappa B(NF-κB)p65 in human breast cancer cells. Method: The MTT assay was used, and the human breast cancer cells (MCF-7) in logarithmic phase of growth were randomly divided into drug group and control group, with the concentration of 0.5,1.5,2.5,3.5 g ·L-1 Banana Peel polysaccharides in MCF-7 cells, Intervention durations were 24, 48,72 h accordingly.The colony formation assay and the western blot technique were used to observe the effects of Polysaccharides on human breast cancer cell colony formation and expression of NF-κB(p65) in breast cancer cells. Test set of drug group and control group, drug group MCF-7 cell with the concentration of 0.5,1.5,2.5 g ·L-1 Banana Peel polysaccharides intervention 48 h. Result: Banana Peel polysaccharides inhibited cell proliferation and induced apoptosis of tumor cells within the drug concentration and drug effects duration of studies. MCF-7 cell colony formation rate, NF-κB (p65) protein expression decreased significantly with a dose-response manner Conclusion: Banana peel polysaccharides inhibited the MCF-7 cells proliferation and colony formation (P<0.05). Polysaccharide banana peel down-regulated the expression levels of NF-κB(p65) protein in MCF-7 cells. The mechanism of MCF-7 apoptosis may be related to down-regulation of the expression levels of NF-κB(p65).关键词：polysaccharides of Banana Peel;breast cancer;proliferation;colony formation;nuclear factor-κB
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Qingkailing in model mice by measuring learning and memory ability and the expression of β-amyloid protein in brain tissue. Method :Male KM mice were randomly divided into 6 groups: Control group; Model group; L-QKL group (20 g ·kg-1); M-QKL group(40 g ·kg-1); H-QKL group (80 g ·kg-1) and Nim group (0.03 g ·kg-1). All the mice were administrated by ig with drugs or water in the same volume for 30 days. AD model of mice was established by ip scopolamine. The Morris water maze was used to observe the learning and memory ability for the model mice. Biochemical methods were used to determine the content of β-amyloid protein in brain tissue. Result: :Qingkailing could significantly improve the memory of model mice compared with the control group, Qingkailing could decrease the content of β-amyloid protein in the brain tissue obviously. Conclusion: Qingkailing could improve the ability of learning and memory of model mice, and its mechanism might involve in reducing the level of β-amyloid protein in brain tissue.关键词：Qingkailing;learning and memory;β-amyloid protein
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发布时间：2024-01-04 - 摘要：Objective: To screen acetylcholinesterase inhibitory activity of total alkaloidal extracted from 8 tibetan medicines. Method: Tibetan medicines were extracted by ethanol and treated with hydrochloric acid and base, and then extracted by chloroform and n-butanol to obtain chloroform and n-butanol-soluble fractions. The AChE inhibitory activity was tested by TLC bioautographic method and modified Ellman's colorimetric method in 96-well microplates. Result: The results indicated that most of the chloroform-soluble fractions among the tested samples exhibited various activities. Holarrhena antidysenterica, Gentiana straminea, Anisodus tanguticus were demonstrated having significant activity with IC50 values of 6.32,28.2 and 34.80 mg ·L-1respectively. The n-butanol-soluble fractions of A.tanguticus also exhibited AchE inhibitory activity with the IC50 value of 51.7 mg ·L-1. Conclusion: H.antidysenterica, G.straminea, A.tanguticus, as sources of AchE inhibitor, are worthy of futher study.关键词：Tibetan medicine;acetylcholinesterase inhibitors;alkaloids;TLC bioautographic method
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidative effect of ethanol extract of Rhaponticum uniflorum. Method : The effect of R.uniflorum ethanol extract on the total antioxidant capacity of liver was determined by ferric reducing method, and the anti-lipid peroxidative activity was detected by thiobarbituric acid (TBA) method. Result :R. uniflorum ethanol extract concentration-dependently elevated the total antioxidant capacity of liver homogenate, inhibited the lipid peroxidation of liver homogenate induced by H2O2, ·OH and Fe2+ with the IC50s of 56.4, 144.2 and 355.6 mg ·L-1, respectively, and inhibited the lipid peroxidation of liver mitochondria induced by H2O2 and Fe2+ with the IC50s of 781 and 36.3 mg ·L-1, respectively. Conclusion: The ethanol extract of R. uniflorum has an antioxidative effect in vitro.关键词：Rhaponticum uniflorum;ethanol extract;antioxidation;anti-lipid peroxidative
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic and antioxidative effect of Boschniakia rossica extract (BRE) combined with vinorelbine (NVB) chemotherapy in VX2-bearing rabbits. Method: The VX2 transplanted rabbit model was established and the rabbits were divided into 4 groups: model, BRE, NVB, BRE+NVB (BRE combined with NVB). Animals in BRE and BRE+NVB groups were treated with 100 mg ·kg-1 ·d-1 BRE i.g. for 21 days from the 8th day, and animals in NVB and BRE+NVB groups were treated with 2.5 mg ·kg-1 ·d-1 NVB i.v. twice on the 15th and 22st day. Then the size and weight of tumor were estimated and the growth inhibition ratio was calculated. The expression of proliferation cell nuclear antigen (PCNA) protein was determined with immunohistochemical method, and the serum activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant activity (TAOC), as well as the content of malondialdehyde (MDA) were detected by colorimetric method. Result: The administration with BRE and/or NVB inhibited the growth of transplanted tumor and the proliferation of tumor cell, while the BRE+NVB group showed the highest growth inhibition ratio and the lowest proliferation index. Furthermore, NVB treatment increased serum MDA content and reduced serum levels of SOD and TAOC despite of the increased activities of CAT and GSH-Px. However, BRE or BRE+NVB treatment increased the serum SOD, CAT, GSH-Px and TAOC, and reduced the serum MDA level in VX2-bearing rabbits. Conclusion: BRE exhibits an inhibitory effect on the growth of transplanted VX2 tumor, and enhances the therapeutic effect of NVB chemotherapy, probably via the inhibition of tumor cell proliferation and the improved antioxidative capability.关键词：Boschniakia rossica;vinorelbine;VX2;proliferation;antioxidation 许惠仙,等:草苁蓉联合长春瑞滨化疗对VX2移植瘤的抑瘤作用及机制研究 第17卷第6期 2011年3月 中国实验方剂学杂志 Chinese Journal of Experimental Traditional Medical Formulae Vol.17,No.6 Mar.,2011
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发布时间：2024-01-04 - 摘要：An improved everted gut sac method was applied to the prescription compatibility effect on absorption of the major components in Rhizoma Coridalis extracts. With the separation and detection, tetrahydropalmatine(THP) and dehydrocorydaline(DHC) were quantified by HPLC-UV. The standard curves of THP and DHC were lineary over the concentration range of 0.29-46.4 and 0.09-22.7 g ·mL-1 respectively. The extraction recovery of both compounds were 95%-100%. The precisions, the accuracy and the stability all met the requirements. The contents of THP did not show difference before and after compatible prescription administrated, where the DHC had a significant increase in the same process. The results revealed that DHC might lead to an increasing pharmacological effect after prescription compatibility.关键词：everted gut sac;Rhizoma Coridalis;combination
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发布时间：2024-01-04 - 摘要：Objective: To explore the change of the expression of apoptotic regulatory protein PKC, Bcl-2 and Bax in spleens of spleen deficiency rats. Method: Sixty SPF rats were divided equally into five groups.Expression of Bcl-2 and Bax in spleens was studied by immunohistochemistry method. Result: Compared to the control group, the expression of Bcl-2 was markedly lower in the model group. Compared to the model group,the level of Bcl-2 was significantly increased while levels of Bax and PKC was significantly decreased. Conclusion: Effect mechanism of Spleen-Invigorating recipe on spleen deficiency may be related to the incresed of Bcl-2 as well as the decrese of Bax and PKC.关键词：spleen deficiency;spleen;PKC;Bcl-2;Bax
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发布时间：2024-01-04 - 摘要：Objective: To separate and purify polysaccharides from root of Cherokee Rose (Jinyinggen,JYG) in Chinese, and investigate its antitumor activity in vivo. Method :Water extracting and ethanol precipitating methods were applied to extract polysaccharide from root of Cherokee Rose. Sevage method was used to remove protein, and macro porous resin absorption was used for decolorization. Mice tumor model was established with transplantion of S180 solid tumor or ascites tumor. And the S180- ascites mice were divided into four groups: control(NS), JYG-Low, medium, high (50, 100, 200 mg ·kg-1). For the mice model with transplanted S180 solid tumor, the mice were divided into eight groups: control(NS),5-Fu(20 mg ·kg-1),JYG-Low,medium,high (50,100,200 mg ·kg-1), JYG-Low,medium,high (50,100,200 mg ·kg-1)+5-Fu(20 mg ·kg-1). Intraperitoneal injection was used to be the major route of administration. The effects of different groups on the inhibitory rate, the proliferation rate,WBC,thymus index(TI) and spleen index(SI) were observed after the mice were sarcrified. Result: The sugar content of JYG polysaccharides was 85.43%.The life-prolonging rates of JYG polysaccharides (low, medium, high) were 3.07%±2.35%,10.11%±4.32%,34.3%±8.31% respectively. There was only significant differences between high dose group and control group. The antitumor effect of JYG polysaccharides-low,meddian,high doses combinated with 5-Fu were superior to 5-Fu, the inhibitory rate were 62.74%±4.35%,67.92%±3.24%,78.79%±6.23% respectively. Compared with 5-Fu alone, the drug combination could increase WBC count, TI, SI and the IL-2 level, but had no effect on TNF-α. Conclusion : The single use of JYG polysaccharides was not effect to inhibit the growth of S180 tumor, but the combination of JYG polysaccharide with 5-Fu had a coordinated effect with better efficacy and lower toxicity.关键词：root of cherokee rose;polysaccharides;antitumor;S180 sarcoma
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发布时间：2024-01-04 - 摘要：Objective : To investigate the effect of extract from Tribulus terrestris on myocardial cell apoptosis caused by adriamycin in rats. Method : Rats were given intraperitoneal injection of adriamycin (ADR) to induce cardiocyte apoptosis. After continuous ig of extract from T. terrestris , myocardial ultrastructure in every experimental group was observed with electron microscope. The apoptotic rate of myocardial cells was determined by terminal deoxynucleotidyl transferaseediated dUTP nick-end labeling (TUNEL), and the expression of Bcl-2 and Bax was detected by using immuno-histochemical method. Result : ADR of 20 mg ·kg-1 induced cardiocyte apoptosis. Extract from T. terrestris middle-and high-dosage groups (40,60 mg ·kg-1) could reduce cardiotoxicity injury by ADR. Extract from T. terrestris middle-and high-dosage groups could significantly increase the Bcl-2 expression ( P <0.01), reduced Bax expression ( P <0.05). Conclusion : Extract from T. terrestris may decrease the cardiocyte apoptosis induced by adriamycin, and it has a protective effect on adriamycin-induced cardio-toxicity.关键词：extract from Tribulus terrestris;adriamycin;Myocardial cells;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of Xinzhi Bimin capsule on the model of rats with allergic rhinitis(AR) and the effect on Interleukin-5(IL-5) and eosinophil(EOS). Method :Fourty rats were randomly divided into control group,sensitized group, the positive control (Beiyankang, 3 g ·kg-1)and experimental groups(3.6 g ·kg-1).The rats were sensitized by ovalbumin. Result : IL-5 content in bone marrow of the experimental group was significantly lower than those of the model group and the positive control group(P<0.05); IL-5 content of blood serum in the experimental group was significantly lower than those of the model group and the positive control group(P<0.05); the numbers of EOS, compared with the control group and the model group,there were significant difference(P<0.05). Conclusion : Xinzhi Bimin capsule can reduce IL-5 content of bone marrow and in serum,and relieve EOS infiltration.关键词：Xinzhi Bimin capsule;allergic rhinitis;interleukin-5;eosinophil(EOS)
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发布时间：2024-01-04 - 摘要：Objective: To select the extraction technigues for Zishen Ningshen decoction. Method: By the experiment of locomotor activity and sleep in mice, four extraction techniques, i.e. water-extraction, alcohol-extraction, water-extraction with alcohol-precipitation, water-extraction and partial alcohol-extraction, were evaluated. Result: The extracts of water-extraction and alcohol-precipitation and water-extraction could significantly decrease the locomotor activity, significantly prolong the sleeping time induced by the under-threshold doses pentobarbital sodium, and the extract of partial water-extraction with partial alcohol-extraction could significantly prolong the sleeping time induced by the threshold doses pentobarbital sodium; and the four extracts all could improve the sleeping rate of the mice, among which water-extraction with alcohol-precipitation extract performed best. Conclusion: The water-extraction alcohol-precipitation was selected as the extraction technique for Zishen Ningshen decoction.关键词：Zishen Ningshen decoction;selection of the extraction techniques;the water-extraction with alcohol-precipitation;pharmacodynamic experiment
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发布时间：2024-01-04 - 摘要：Objective: To study antifungal action in vitro of extracts from Garlic by supercritical fluid extraction (SFE). Method: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of SFE extract of Garlic against 15 strains dermatophytes were determined by media dilution method. Result: MIC of SFE of garlic to Shanghai strain (Trichophyton tousurans) and Wuhan strains (Trichophyton memtigrophytes, Wool microsporum canis, Achromatism trichophyton rubrum) were 3.13 mg ·L-1, MIC to Shanghai strains(Plaster microsporum canis, Wool microsporum canis)and Wuhan strain(Wool microsporum canis) were 1.56 mg ·L-1, MIC of SFE to Shanghai strains (Trichophyton cerebriforme, Trichophyton rubrum, Trichophyton gypserum) and Wuhan strains (Trichophyton gypserum, Trichophyton rubrum, Microsporum canis, Candida albicans ) were less than 0.78 mg ·L-1. MBC values were different. Apart from MBC of SFE of garlic to Shanghai strains(Plaster microsporum canis, Epidermophyton floccosum)and Wuhan strains (Trichophyton mentagroph, Epidermophyton floccosum) were 6.25 mg ·L-1, MBC to Shanghai strains (Trichophyton tousurans, Wool microsporum canis) and Wuhan strains (Trichophyton rubrum, Achromatism trichophyton rubrum, Microsporum canis) were 3.13 mg ·L-1, MBC to Trichophyton cerebriforme(Shanghai strain)was 1.56 mg ·L-1, MBC to Shanghai strains (Trichophyton rubrum, Trichophyton gypserum) and Wuhan strains (Trichophyton gypserum,Candida albicans) were less than 0.78 mg ·L-1. Conclusion: The SFE extract of Garlic showed strong antifungal effect in vitro.关键词：garlic;supercritical fluid extraction;dermatophytes
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of local application of Qingpeng Paste for treating adjuvant arthritis (AA) in rats. Method: Ten rats was eandomly selected form fifty rats as the normal control, the other rats were used to made the adjuvant arthritis model. The model was induced by injection of Freund’s complete adjuvant. Then, the fourty rats were divided into model group, Qingpeng Paste high dose group (1.6 g ·kg-1) and Qingpeng Paste low dose group (0.4 g ·kg-1). The influence on swelling of the rear leg, the serum nitric oxide (NO) and interleukin-1β(IL-1β) level in the adjuvant arthritis rats were observed. HE staining was used to examine the pathological changes in joint synovium of the rat after using Qingpeng Paste locally. Result: Qingpeng Paste significantly decreased swelling of joints in AA rats compared with the control (P<0.05). the levels of NO of the rats in Qingpeng Paste groups were significantly decreased compared with the control (P<0.05). However, the level of IL-1β did not change. The pathological changes un the synovium tissues were markedly ameliorated in Qingpeng Paste groups. Conclusion: Qingpeng Paste can ameliorate the symptom of adjuvant arthritis in rats.关键词：Qingpeng Paste;adjuvant arthritis in rats;nitric oxide;interleukin-1β
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Shengmaiyin and its crude compositions extract on isolated normal and failure heart model. Method : Failure model of isolated heart was made by ligating coronary artery, then the hearts were perfused by Working Heart system. Polygraph was used to determine cardiac function affected by different drugs. Result : Shengmaiyin of different concentrations could cause the left ventricular systolic pressure, the maximum rise or decline rate of left ventricular pressure and coronary blood flow to increase in a concentration-dependent manner. Renshenfang was a little better than Dangshenfang. As compositions of Shengmaiyin, Hongshen and Ophiopogonic Radix had a positive effect on cardiac function, of which two parts of Ophiopogonis Radix crude extracts, Maidong-30 and saponin all had a great effect on left intraventricular pressure maximum alleosis rate. Conclusion : The main materials of Shengmaiyin affected positively for isolated failure heart are two compositions of Ophiopogonic Radix:Ophiopogonic Radix-30 and saponin.关键词：Shengmaiyin;isolated failure heart;rat;Ophiopogonic Radix
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发布时间：2024-01-04 - 摘要：Objective: To investigate whether schizonepetin has any influences on the cardiovascular system, the nervous system and the respiratory system of animals. Method: Mice and rats were given oral medicine with different doses of ST in order to evaluate the effects of ST on sleep and the autonomous movement in rats, on the falling number of mice from the rotating stick. Anesthetized dogs were given oral medicine with different doses of ST in order to evaluate the effects of ST on the frequency and depth of respiration, systolic and diastolic blood pressure (SAP, DAP), heart rate (HR), PR, QRS, QT and T-wave. Result: With the dosage, ST had no obvious effect on the number of sleep and the autonomous movement in rats,on the falling number of mice from the rotating stick. With the dosage, ST had no obvious effect on the frequency and depth of respiration, systolic and diastolic blood pressure, heart rate, PR,QRS,QT and T-wave. Conclusion: Experimental dosage for mice, 20 times of the clinical adult dosage, is the half dose of LD50 for mice. The study indicates that at this level of dosage ST has no obvious effects on respiratory, cardiovascular and nervous systems.关键词：schizonepetin;general pharmacology;cardiovascular systems;respiratory systems;central nervous system
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effects of compound Sanguisorba officinalis tincture on burns. Method : The compound tincture, a hospital preparation, was prepared according to Jiangxi hospital preparation Norms. Four-hundred ninety-six patients suffered from burn were divided into treatment group and control group randomLy, and each wound was debrided and exposed. The treatment group was treated with compound S. officinalis tincture, while the control group was cured with SNO (silver nitrate ointment) combined with antibiotics. Result : The effective rate of treatment group and control group was 100% and 94.6% respectively, and the average cure time was 12.7 d and 31.3 d respectively. There was significant difference between the two groups ( P <0.01). Conclusion: Compound S. officinalis tincture is effective for the treatment of I and II burns in short treatment duration and with less adverse reactions, and it is reasonable priced.关键词：compound Sanguisorba officinalis tincture;treatment;burns
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发布时间：2024-01-04 - 摘要：Objective: To study the clinical efficacy of carotid artery injection of ligustrazine phosphate injection for treatment of cerebral infarction. Method: Two-hunrend and fourty patients (45 to 65 years) surffered from cerebal infarction were randomized into treatment group (120 cases) and control group (120 cases). Treatment group was taken with the convientional treatment supplemented with carotid artery injection of ligustrazine phosphate, and the control group was only taken convientional treatment supplemented with vein injection of ligustrazine phosphate. Two groups were observed with integrated effects, neurological deficit score, area of cerebral infarction etc, before and after treatment. Result: The total effective rate of carotid artery injection was 94.2% (113/120). The total effective rate of the control group was 85.8% (103/120). No case with adverse reactions was found in treatment group. The neurological deficit score and area of cerebral infarction in the treatment group was better than that in the control group (P＜0.05). The shorter the disease history, the better the treatment showed. Conclusion: Carotid artery injection of phosphate ligustrazine can reduce neurological deficit score and area of cerebral infarction.关键词：cerebral infarction;ligustrazine phosphate injection;carotid artery injection
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Dingchuan decoction combined with airway inhalation of corticosteroids and β 2 agonist for treating children with acute attack of bronchial asthma (heat asthma, TCM term). Method: Eighty cases of heat asthma with symptom of bronchial asthma in children were randomLy divided into control group and treatment group, Thirty-eight patients in the control group were treated with pulmicort and salbutamol with pump. The other forty-two patients in the treatment group were treated with pulmicort and salbutamol with pump combined with oral Dingchuan decoction. Three days were considered as a course of treatment. Result : The total effective rate was 97.6％ in treatment group and 84.2％in control group, The difference had statistical significance ( P <0.05). Conclusion: Dingchuan decoction combined with airway of inhaled corticosterioids and β2 agonist for treating children with bronchial asthma (asthma heat) appears to be significant effective. The therapeutic approach might be widely applied.关键词：asthma;Dingchuan decoction
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Jiemin Granules (JMG) on allergic thinitis based on a clinical trial. Method: Sixty cases with allergic thinitis (AR) were randomly divided into two groups equally. i.e. treatment group(TG) treated by JMG and control groups(CG) treated by Loratadine Tablets (LT). Both interventions lasted for two weeks. Then, therapeutic effects were evaluated for the short-term (the 1st day) and the long-term (the 15th day). Result: On the 1st day following the therapy finished, the total effective rate was 90% for the cases of TG, while it was 93% for those of CG, with no statistical significance between them. By the 15th day after the therapy, the improving rate was 83% in TG, in contrast, the rate was only 70% in CG, with marked statistical significance( P ＜0.05)between them. Conclusion: The short-term therapeutic effect of JMG is similar with that of the LT, while the long-term therapeutic effect is better than that of LT. It holds a delaying effect on disease recurrence and is worthwhile for the JMG to be promoted into practical usage in the treatment of AR.关键词：allergic thinitis;Jiemin Granules;observation of therapeutic effect
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发布时间：2024-01-04 - 摘要：Objective: To study the therapeutic effect of citalopram combined with Shenshuaining pill (SSNP) in the treatment of post-stroke depression. Method: Eighty post-stroke depression patients were randomly assigned into treated (citalopram combined with SSNP) and control (citalopram) groups (each n =40) for 6 weeks.Anti-depression therapeutic effect, neural functional impairment and adverse reactions were assessed with the hamilton depression scale (HAMD), the modified Edinburgh-Scandinavia stroke scale (MESSS), and the Treatment Emergent Symptom Scale(TESS)respectively. Result: In the treatment group, the scores of the HAMD and TESS distinguished markedly at the end of the 1st and 2st weeks, and the total scores of the MESSS were 14.22±4.53 in treatment groups at the end of the 6th were, compared with control group(17.38±5.10), they decreased significantly distinguished( P <0.05). Conclusion: Citalopram combined with SSNP had superior therapeutic effect for neural function recovery, and was a kind of rapid and safe drug in the treatment of post-stroke depression.关键词：citalopram;Shenshuaining pill;post-stroke depression
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect on the treatment for gastroesophageal reflux disease with reinforcing spleen and stomach. Method: One hundred and six cases are randomly divided to the therapy group (56 cases) and the control group (50 cases). The therapy group was treated by Chinese materia medica decoction. the control group was treated by omeprazole and domperidone. The course of treatment was 30 days. The follow-up was done 30 days after the treatment. Result: After a course of treatment, the effective rate of two groups was 98.2% and 94.0% respectively; the effective rate of the therapy group was higher than that of the control group, the difference between two groups had a distinct significance(P<0.05). The therapy group obviously improved scores of each symptom and reflux diseases questionnaire. Conclusion : The effect of the treatment for gastroesophageal reflux disease with reinforcing spleen and stomach is sure.关键词：gastroesophageal reflux disease;reinforcing spleen and stomach;reflux diseases questionnaire
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发布时间：2024-01-04 - 摘要：Objective: To investigate drug-induced adverse reactions by analyzing clinical manifestations. Method: Using retrospective methods the clinical drug adverse reactions were analyzed. Result :Based on data of 452 cases,we learnt obviously that the incidence rate in the old is higher than that in the young.The antibiotic drugs account for most adverse reactions,and adverse reactions occur in several systems. The adverse reactions in skin is the highest, while most are slight. Conclusion: Adverse reactions still exist at large in clinic, In order to avoid or reduce the incidence of the reactions, drugs should be used reasonably with caution in patients.关键词：adverse drug reactions;cases analysis
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发布时间：2024-01-04 - 摘要：The literatures on medicine and herbs from ancient time to modern time were collected and studied, and the historical development of methods for processing Fluoritum and the survey on processing in different provinces were summarized. From Liang Dynasty to Qing Dynasty, 12 processing methods were recorded, and the processing technique with fire and vinegar was applied widely. The traditional processing theoretic was gaining in the long-term of history. In present days, the processing technique with fire and vinegar is practiced country-widely, and other techniques appear to be less-applied.关键词：fluoritum;processing methods;historical development
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发布时间：2024-01-04 - 摘要：The alzheimer disease (AD) pathological models were collected to provide new ideas and methods, including main methods for establishing AD model, i.e. the physical methods, the chemical methods the multi-factors methods, transgenic AD model and natural aging AD model. From the data, we realized that there were many kinds of methods for AD establishment. Each model either at whole animal level or at cellular level has its advantages and disadvantages, with different underline mechanisms. Researchers should select the suitable method according to the need of their experiments.关键词：Alzheimer disease;animal model;cellular model
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发布时间：2024-01-04 - 摘要：The flavonoid compounds have very powerful antioxidant and remove the active ability, can prevent free radicals attackmg DNA and inducing cancer. In cardiovascular system, endocrine system, immune system and antitumor aspects significant pharmacological activities which very ectensive prospect on the duelopment and application the feavondas have. This paper mainly describes the recent natural flavonoids of the extraction process of research, compare to its at the same time. It is concluded that the ultrasonic method with microwave-assisted extraction is simple operation in with short time, can consider to appliy in industrial production scale.关键词：flavone;quantitative analysis;extraction process
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发布时间：2024-01-04 - 摘要：Naodesheng is composed of Panax Notoginseng, Rhizoma Chuanxiong, Flos Carthami, Radix Puerariae, Crataegus pinnatifida. It has wide pharmacological actions on the cardiovascular system. Also, it has wide clinical application and good therapeutic effect. The literatures on quality control, pharmacological actions and processing procedure of Naodesheng in recent twenty years are analyzed and discussed.
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发布时间：2024-01-04 - 摘要：Glucocorticoid is an effective medicine to cure inflammatory disease in clinic, but the glucocorticoid resistance always takes place. Modern medicine thinks that the resistance has relevant with glucocorticoid metabolism, the gene change of glucocorticoid receptor and the histone acetylase and deacetylases of target gene, etc. On the other hand, Traditional Chinese medicine regards the resitance related with the yin and yang deficit of kidney. This review is to summarize the pathogenesis progress of glucocorticoid resistance in both modern medicine and traditional chinese medicine.关键词：glucocorticoid;glucocorticoid resistance;pathogenesis
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发布时间：2024-01-04 - 摘要：Chinese patent medicines have good therapeutic effect in common rheumatic diseases treatment such as rheumatoid arthritis, systemic lupus erythematosus, Sjogren’s syndrome, ankylosing spondylitis, osteoarthritis, primary osteoporosis and so on. As an adjuvant for antirheumatic medicine, Chinese patent medicines could not only improve the efficacy but also reduce the dosage of anti-rheumatic western medicines and to reduce their adverse reactions. It will help to improve the therapeutic effect that choosing a proper Chinese patent medicine according to patients’ different pathological stage and the symptom differentiation.关键词：Chinese patent medicines;rheumatic diseases;therapentic effect
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