最新刊期
卷 17 ， 期 7 ， 2011
- 摘要：Objective: To explore the factors played important roles in Euodiae Fructus processing. Method: 2×2 factorial design was used in the processing with heating and adjuvant as independent variable. Evodin, evodiamine and rutaecarpine in Evodia were determined and analyzed by SAS. Result: Evodiamine and rutaecarpine in heating products were significantly higher than non-heating ones (P<0.05); evodin increased, but had no significant difference. Evodin in products without licorice was significantly higher than products with licorice (P<0.05); evodiamine and rutaecarpine showed no significant difference with or without of licorice. Heating and adjuvant had remarkable interaction to evodin (P<0.01), but not significant to evodiamine and rutaecarpine. Conclusion: The heating was the main factor in Euodiae Fructus processing, while licorice juice had little effect on the content of the three components in Euodiae Fructus measured in this study.关键词：Euodiae Fructus;processing;heating;adjuvant;factorial design
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发布时间：2024-01-04 - 摘要：Objective: To establish the best processing technique of Kansui Radix stir-baked with vinegar. Method: The best processing technique was gained through determining the content of euphol by HPLC as the index, and orthogonal design experiment was used to investigate the factors of vinegar amount, baked time and temperature. Result: The best processing technique was to add 30% vinegar and bake 7 minutes at 260 ℃. Conclusion: This best processing technique is stable, feasible with good repetition.关键词：Kansui Radix stir-baked with vinegar;processing technique;Orthogonal Design
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发布时间：2024-01-04 - 摘要：Objective: To optimize the water extraction conditions for solid-fermented Angelica sinensis. Method: Orthogonal design was used in optimizing the extraction conditions while ferulic acid and extract dry weight were adopted as markers. Result: Optimum extraction technology was as follows: the medicinal materials were soaked in water for 0.75 hour, and extracted 2 times with one hour for each time by 12-fold and 8-fold volume of water. Conclusion: Extraction technology established in this experiment is stable and feasible and can be used to extract solid-fermented Angelica sinensis.关键词：Angelica sinensis;solid fermentation;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: to optimize the cutting technology of Aucklandiae Radix. Method: The cutting technology of Aucklandiae Radix was optimized by L9 (34) orthogonal design taking the content of costunolide and dehydrocostuslactone, the intestinal propulsion in mice as indexes with the amount of water, soaking time and thickness of pieces as factors. Result: The best cutting technology of Aucklandiae Radix was cutting 1-2 mm thin pieces after soaking 10 h with 0.3 times water. Conclusion: The optimum cutting technology of Aucklandiae Radix is reasonable and feasible; it can provide a reference for establishing the quality standard of Aucklandiae Radix pieces.关键词：costunolide;dehydrocostuslactone;intestinal propulsion;cutting technology
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发布时间：2024-01-04 - 摘要：Objective: To establish an appropriate and technical process for the purification of Arctium lappa by macroporous resins. Method: The HPLC was used to analyze the content of arctiin in A.lappa, and the adsorption behavior of macroporous resins to arctiin in A.lappa was examined for the adsorption rate and desorption rate. Result: The D101 resin was the suitable for enriching arctiin. The sample concentration of arctiin was 28.69 g ·L-1 with the elution flow rate of 3 BV ·h-1. 5 BV water and 10 BV 30% ethanol were used as eluant of arcttin, respectively. Portions of 30% ethanol were collected, concentrated, and dried. Conclusion: This method is simple and feasible with good effect of enrichment in arctiin.
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发布时间：2024-01-04 - 摘要：Objective: To select optimal molding technique of Aitongxiao granules by orthogonal design. Method: The main influential factors of the molding technique included the proportion between mixed subsidiary materials, CMC-NA dosage, ethanol concentration. Conditions of the molding were evaluated with formation rate, hygroscopic and color uniformity as indexes. The optimal technique of Aitongxiao granules was selected by L9(34) orthogonal design. Result: The best molding technique was that the proportion between mixed subsidiary materials (dextrin to soluble starch) was 2 ∶1, 2%CMC-NA, the wetting agent was 85% alcohol. Conclusion: The forming technology is feasible,stable,reasonable and reproducible and can serve as theoretic basis for industrial production.关键词：orthogonal experiments;Aitongxiao granules;molding technique
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发布时间：2024-01-04 - 摘要：Objective: research on the process parameters of microwave vacuum drying technology of Jinshao capsule and its manufacturing feasibility. Method: Microwave vacuum drying technology was select to dry the concentrated liquid extraction of Jinshao capsule and to determine the moisture content, the retention rate of paeoniflorin and berberine hydrochloride. Result: Accessories required for microwave drying process with the shorter retention, retaining the active ingredients better. Conclusion: Microwave drying process is suitable for the drying of Jinshao capsule.关键词：Jinshao capsule;microwave vacuum drying process;technology
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发布时间：2024-01-04 - 摘要：Objective: To establish the techniques for the extraction of dracorhodin from Draconis Sanguis. Method: With the content of dracorhodin as the index, observations were made on the effect of different methods of extraction by HPLC. Result: High content of dracorhodin was obtained by SFE-CO2 relatively. Conclusion: SFE-CO2 is a workable technique for the extraction of dracorhodin from Draconis Sanguis.关键词：Draconis Sanguis;dracorhodin;extraction;SFE-CO2;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the extraction technological parameters of the total alkaloids from Lycopodium japonicum. Method: The orthogonal test was used to optimize extracting conditions in the process of acid water extraction. Then the optimized conditions for purification using cation exchange resin were investigated. Result: The optimized conditions in the process of acid water extraction were 0.5% hydrochloric acid as much as 15 times of the medicine amount for 24 h and three times. Then the extraction of acid water was purified with a column of macroporous cation exchange resin 001×7 at 3 BV ·h-1 of flow rate, then eluted with 10 BV of 8% aqueous ammonia ethanol. Conclusion: This technology is rapid and accurate for the purification of total alkaloids from L.japonicum.关键词：Lycopodium japonicum;total alkaloids;cation exchange resin
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发布时间：2024-01-04 - 摘要：Objective: Screen the extraction process of Tongfengding granules by the mouse model of hyperuricemia. Method: Given by gavage uricase inhibitor potassium oxide hydrochloride manufacture of high uric acid (UA) mouse model of hyperlipidemia, the mice were fed grain with water extraction, water extraction and alcohol precipitation, 70% ethanol extracts derived from extracts of Tongfending graules,and then the concentrations of uric acid in the blood of each group (SUA) were determined by UV spectrophotometer. Result: Compared with normal group, the serum uric acid levels in model group mice were significantly higher (P<0.001);compared with model group, the water extract obtained extract could significantly reduce the high uric acid levels in mice serum uric acid (P<0.01); supernatant and precipitation part in water extraction and alcohol precipitation had no significant impact on serum uric acid levels of high serum uric acid mice; 70% ethanol extracts could significantly increase serum uric acid levels in model group mice (P<0.001). Conclusion: Water extraction from the clinical setting for Tongfengding graules is the best particle extraction.关键词：Tongfengding granules;extraction process;hyperuricemia;potassium oxide hydrochloride
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发布时间：2024-01-04 - 摘要：Objective: To optimize the water extraction process of Xinshuaining Mixtures. Method: Orthogonal design was used to select the water extraction process of Xinshuaining Mixtures with the content of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and the yield of extract as the investigating index. Result: The best water extraction process of Xinshuaining Mixtures was: the first time adding 12 folds water, adding 10 folds water for second and third times, decocting 1 h for 3 times. Conclusion: The established technology was reasonable, feasible and reproducible. It can be used for the development of Xinshuaining Mixtures to provide theoretical and experimental basis.关键词：Xinshuaining Mixtures;water extraction process;Ginsenoside Rg1;Ginsenoside Re;ginsenoside Rb1;ortthogonal experiment
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发布时间：2024-01-04 - 摘要：Objective: To study comparatively on ultramicro powder of Tianqitongjing capsule and common Tianqitongjing capsule. Method: The dissolution rate of the two kinds of capsules was determined according to relating dissolution rate determination method on China pharmacopoeia. Zero angle of the two kinds of capsules was determined by powder flow ability determinator. The effect of the two kinds of capsules was compared on uterus and ovaries developing of mouse, i.e. the effect of xylene to swelling of ear of mouse and the effect of oxytocin to writhing response of rat. Result: Every target of ultrmicro powder of Tianqitongjing capsule was better than common Tianqitongjing capsule. The ultramicro powder could get to the identical drug action at the dosage of 1/3 comparing with the common Tianqitongjing capsule. Conclusion: The examination laying a foundation for the developing of ultramicro powder technology.关键词：ultramicro powder;Tianqitongjing capsules;dissolution rate;drug action comparison
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发布时间：2024-01-04 - 摘要：Objective: explore the best drying temperature and drying time of Gardeniae Fructus through the study of its drying process optimization, so as to control the quality of Gardenia. Method: To study the drying temperature and drying time on the Gardeniae Fructus geniposide content using high performance liquid chromatography with mass fraction of geniposide index, considering the drying temperature and drying time, and the single factor for optimization of Gardeniae Fructus drying process. Result: Gardeniae Fructus geniposide content is high and stable at 50 ℃; gardenia fruit geniposide showed a marked downward trend above 65 ℃. Conclusion: The drying process Gardeniae Fructus is fast and quality assurance for 36 hours at 50 ℃.关键词：drying process;Gardeniae Fructus;single factor design experiment
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发布时间：2024-01-04 - 摘要：Objective: To study the method of enrichment of fumaric alkaloids by using cation resin. Method: Ultraviolet spectrophotometry was used to determine total fumaric alkaloid with exchange capacity as index. Result: The optimum purification condition was as follows: herbs: concentrate solution was about 1 ∶5, herbs: cation resin was about 1 ∶1; flow rate was 2 BV ·h-1, diameter:high of cation resin column was about 1 ∶10; 5 BV of purified water eluted resin column to removed impurities, flow rate was 2 BV ·h-1, 4 BV of 70% ethanol as eluent consisted of 5% ammonia, 2 BV ·h-1 flow rate washing resin column. Conclusion: The optimum condition was stable and can be used for purification of alkaloids.关键词：Corgdalis Rhizoma;resins;ultraviolet spectrophotometry;separation and purification
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发布时间：2024-01-04 - 摘要：Objective: To achieve rapid quantitation of active components in Tongmai granule. Method: By choosing Kinetex C18 (3 mm×50 mm, 2.6 μm) as analysis column, an HPLC-UV method was established to detect puerarin and ferulic acid in Tongmai Granule under detection wavelength of 313 nm. The mobile phrase was optimized as 10% acetonitrile-1% acetic acid (54 ∶46) at flow rate of 0.6 mL ·min-1. Result: Under this analytic condition, the quantitation could be accomplished within 10 minutes profiling good separation and specificity of puerarin and ferulic acid which presented retention time at 5.7, 7.6 min.The average recovery was (98.1±1.40) %, (103.7±1.68) % for puerarin and ferulic acid respectively. Calibration curves ranged from 10 to 100 mg ·L-1 for puerarin and from 0.05 to 0.5 mg ·L-1 for ferulic acid with correlation coefficients of 0.999 7 and 0.999 3. Conclusion: The method is rapid, sensitive, accurate and precise. It could be used to provide a rapid determination in quality control of Tongmai granule.关键词：Tongmai granule;puerarin;ferulic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of camphor, menthol, borneol and methylsalicylate in Hongyao strapping. Method: Capillary gas chromatography was adopted. The chromatography separation was carried out with PEG-20M capillary column(0.25 μm×0.25 mm×30 m), using a flame ionization detector. The injector and detector temperature were set at 200 ℃. The column temperature was 160 ℃. n-Octanol was used as internal standard. The sample solution was prepared by distillation with essential oil collector. Result: The resolution and linearity of camphor, menthol, borneol and methylsalicylate were good under the chromatographic conditions. The average recoveries of camphor, menthol, borneol and methylsalicylate were 99.8%, 100.3%, 99.5%, 99.7%. Conclusion: This method is simple, sensitive, accurate and has good repeatability for controlling the quality of camphor, menthol, borneol and methylsalicylate in Hongyao strapping.关键词：CGC;Hongyao strapping;camphor;Menthol;borneol;methyl-salicylate;Assay
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发布时间：2024-01-04 - 摘要：Objective: To study the cleaning mechanism of Aconitum carmichaeli. Method: Titration method was used to determine the content of total alkaloids; UV was used to determine the content of ester-type alkaloids; HPLC was used to determine the content of three kind of diester alkaloids namely aconitine, hypaconitine, mesaconition; sulfuric-phenol method was used to determine the content of polysaccharides. Result: The content of total alkaloids in parent root, daughter root, rootlet of Aconitum carmichaeli was 11.20, 11.70, 18.40 mg ·g-1 respectively; the content of ester-type alkaloids was 4.41, 3.33, 8.24 mg ·g-1 respectively; the content of three kind of diester alkaloids was 0.90, 1.07, 2.18 mg ·g-1 respectively; the content of polysaccharides was 60.60, 98.20, 61.80 mg ·g-1 respectively. Conclusion: The categories of main chemical compositions of parent root, daughter root and rootlet have no obvious difference, but the content of them is different. The sum of content of three kind of diester alkaloids of rootlet is more than twice that of parent root.Cleaning has scientific value.关键词：Aconitum carmichaeli;cleaning;alkaloid;polysaccharide
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method for senegenin and polygalacic acid in Naolibao pills by capillary electrophoresis. Method: The separation was carried out in an uncoated fused silica capillary (75 μm×50 cm). The running voltage was 25 kV. The buffer was 20 mmol ·L-1 Na2B4O7 containing 20% methanol. The wavelength was at 210 nm. Result: senegenin was linear in the range of 13.25-212 mg ·L-1(r=0.997 1) and polygalacic acid was linear in the range of 12.5-200 mg ·L-1 (r=0.997 7). The average recoveries of senegenin and polygalacic acid in Naolibao pills were 100.5% and 100.6% respectively. Conclusion: This method is proved to be simple, rapid and accurate, and can be used as a new method for the quality control of Nailibao pills.关键词：senegenin;polygalacic acid;capillary electrophoresis;Naolibao pills
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components of the essential oil of the Asarum from various habitats and provide scientific basis for its quality control. Method: The essential oil was extracted by water-steam distillation. The relative content of chemical components was identified by GC-MS method. Result: Three batches of A. from various habitats were determined and 18 common characteristic compounds were identified, mainly including the methyl eugenol, safrole, estragole, 3,5-dimethoxy-toluene, α-pinene, β-pinene, 3-carene, eucarvone, myristicin, and elemicin. Conclusion: The essential oils of A. from various habitats are different in the ratio and level of main components.关键词：GC-MS;Asarum;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: An HPLC method was established for the determination of evodin, evodiamine and rutaecarpine in Evodia rutaecarpa and the processed product. Method: RP-HPLC method was established with an Agilent C18 column (4.6 mm ×250 mm, 5 μm). The mobile phase was acetonitrile and water (45 ∶55). The detective wavelength was at 225 nm. Result: There were obvious differences of the content of three components in E. rutaecarpa and the processed product. Conclusion: The different processing methods have certain effect on the content of evodin, evodiamine and rutaecarpine. It can provide evidence for the clinical use.关键词：Evodia rutaecarpa;processing methods;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To estalish an accurate method for the determination of baicalin in Qinqiao Kanggan granules. Method: Diamonsil C18(4.6 mm×250 mm,5 μm)column in an oven at 30 ℃was need,with a mobile phase consisting of mathanol-0.1% phosphoric acid(48 ∶52) and a UV detector at 280 nm, the flow rate was 1.0 mL ·min-1. Result: The linear ranges of baicalin was 0.49-2.46μg(r=0.999 9).The RSD of measurement precision test was 0.25%; The average recovery was 100.54% (RSD 1.89%, n=5). Conclusion: The method was simple and accurate, and can be used for the quality control of Qinqiao Kanggan granules.关键词：Qinqiao Kanggan granules;baicalin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of Huoxue Yangyin granules composed of Herb Leonuri, Radix Puerariae Lobatae. Method: The quality of each raw material was identified by TLC. The quantity of the preparation was conducted by HPLC and the method was developed with the condition as follows: Alltima C18 (4.6 mm×250 mm, 5 μm), mobile phase methanol-0.3% phosphoric acid (26 ∶74), the temperature of column 35 ℃, flow rate 1.0 mL ·min-1, calculating the content with external standard method. Result: Herb Leonuri, Radix Puerariae Lobatae could be detected by TLC. Puerarin was linear in a range of 0.041 8~1.002 2 μg (r=0.999 9). The average recovery was 99.4%, and RSD was 1.4% (n=6). Conclusion: This method is simple, reliable, accurate, and can be used to control the quality of Huoxue Yangyin granules.关键词：Huoxue Yangyin granules;Herb Leonuri;Radix Puerariae Lobatae;puerarin;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for simultaneously determining dehydrocavidine,palmatine hydrochloride and berberine hydrochloride in total alkaloids of Corydalis saxicola. Method: An Agilent XDB C18 column (4.6 mm×150 mm,5 μm) was used, the acetonitrile-0.01 mol ·L-1 potassium dihydrogen phosphate solution(18.5 ∶81.5)as mobile phase, the column temperature was at 30 ℃, the detection wavelength was set at 347 nm,the flow rate was 1.0 mL ·min-1. Result: The liner range of dehydrocavidine was 15.375-92.25 mg ·L-1 (r=0.999 9,n=6)and the average recovery was 96.43%(RSD 1.13%).The liner range of palmatine hydrochloride was 14.91-89.46 mg ·L-1 (r=0.999 9,n=6) and the average recovery rate was 96.28%(RSD 1.22%).The liner range of berberine hydrochloride was 3.525-28.2 mg ·L-1 (r=0.999 7,n=6).and the average recovery rate was 103.02%(RSD 1.35%). Conclusion: The method appeared to be accurate, sensitive and reliable,and can be used to control the quality of the total alkaloids of C.saxicola.关键词：total alkaloids of Corydalis saxicola;HPLC;dehydrocavidine;palmatine hydrochloride;berberine hydrochloride
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of methamidophos in herbs. Method: The content of methamidophos in herbs was determined by UPLC-MS/MS. The chromatographic analysis was performed on an Acquity UPLC BEHC18 (2.1 mm×50 mm, 1.7 μm) by a gradient elution employing of acetonitrile-0.1% formic acid. The analytes were detected using electrospray ionization tandem mass spectrometry with multiple reaction monitoring (MRM) in positive ion scan mode. Result: The linear range of methamidophos was 0.5-10.0 mg ·L-1. The average recovery was 98.53% and RSD was 2.1%. Conclusion: The method was simple, sensitive, accurate and short of analytical cycle.关键词：ultraperformance liquid chromatography (UPLC);MS;methamidophos
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard for Ganfukang tablets. Method: Hedyotis difusa and Pseudostellaria were identified by TLC. Schisandrin was determinated by HPLC. The chromatographic condition was as follows: column of Agilent HC-C18 (4.6 mm×250 mm, 5 μm), mobile phase consisting of acetonitrile-H2O (55 ∶45), flow rate of 1.0 mL ·min-1, and detection wavelength of 250 nm. Ferrous sulphate was determinated by spectrophotometry. Result: The characteristic identification by TLC was distinct and highly specific. Schisandrin was linear in the range of 0.112-5.644 μg (r=0.999 98), the average recovery was 97.03% (RSD 1.38%) with precision RSD of 1.5% (n=6), repetition RSD of 1.65% (n=6) and stability RSD of 1.43%. Conclusion: The method is reliable, accurate, specific and can be used for quality control of the production.关键词：Ganfukang tablets;schisandrin;Hedyotis difusa;Pseudostellaria;HPLC;TLC
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发布时间：2024-01-04 - 摘要：Objective: To establish the determination method of hyperin content in Hypericum attenuatum Choisy. Method: VP-ODS Cl8(4.6 mm×150 mm, 5 μm)colmnn was used,the mobile phase was acetonitrile-0.1% phosphoric acid water(16 ∶84);with the flow rate as 1.0 mL ·min-1, the detection wavelength was at 360 nm. Result: The linear relation of hyperin was good in the range from 0.099 8 to 0.998 0 μg.The average recovery was 100.11%,RSD 1.20%. Conclusion :The method established is accurate,sensitive and good in reproducibility.It can be applied to the qualitycontrol of H.attenuatum关键词：Hypericum attenuatum Choisy;hyperin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To synthesis of the antiplatelet drug triflusal. Method: Triflusal was prepared from 4-trifluoro methyl salicylic acid by one step. Result: Its structure was confirmed by NMR, MS, IR, EA. The total yield of triflusal with the purity of 99.5% by HPLC was 65.4%. Conclusion: The synthetic route for triflusal was simple, feasible and convenient for industrial manufacture.关键词：triflusal;synthesis;structural identification
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发布时间：2024-01-04 - 摘要：Objective: To explore the feasibility of quality control index of processed alisma pieces. Method: The content of 24-acetyl alisol A and 23-acetyl alisol B in processed alisma pieces was determined by high performance liquid chromatography; appearance information of processed pieces was gotten by cameras and Adobe Photoshop software, to evaluate the shade diferential of different origin. Result: The total amount of 24-acetyl alisol A and 23-acetyl alisol B in every processed alisma were more than 0.08%. Color uniformity of processed alisma was between 10 to 2.5, and the processed alisma chromatic diferential was in the 2-8 chromatic units. Conclution: The quality control indicators to reflect the intrinsic quality of processed Alisma Pieces can be used for quality control of traditional Chinese medicine alisma.关键词：Alismataceae;alisol B-23 acetate;alisol A24-acetate;external color difference of processed pieces
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发布时间：2024-01-04 - 摘要：Objective: To eatablish HPLC fingerprints of Citrus nobilis var.chachikan Wong and C. reticulata Blanco and to provide a base for the identification of its quality. Method: A gradient separated method was applied. ODS-C18 column (4.6 mm×250 mm, 5 μm), mobile phase was methanol-water, detection wavelength was at 300 nm, flow rate was 1.0 mL ·min-1,column temperature was at 25 ℃. Result: Eight mutual peaks in C. nobilis var.chachikan Wong and eight mutual peaks in its preparations were separated effectively, the same as C. reticulata Blanco. The methodological evaluation showed that this method had a good repeatability. Conclusion: The results of peak areas were in accordance with the request of fingerprint. The established fingerprint can be used for the quality control and species identifying of C. nobilis var.chachikan Wong, C. reticulata Blanco and its preparations.关键词：C. nobilis var. chachikan wong;Citrus reticulata Blanco;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components of essential oil from leaves and fruits of Lindera glauca. Method: The essential oil was collected by solid-phase microextration (SPME). The chemical constituents were separated and identified by gas chromatography-mass spectrometry (GC-MS) combined with Kovats index. Result: Twenty-five and thirty-three species of chemical components were identified by GC-MS. The content of the identified compounds extracted from leaves and fruits were 96.51% and 99.73% of the total volatile substances, respectively. D-germacrene (45.56%), (+)-ledene (5.76%) and caryophyllene (5.75%) were the most abundant volatile compounds released from leaves. The main chemical components of the fruit were (Z)-3,7-dimethyl-1,3,6-octatriene(31.90%), copaene (12.75%), α-caryophyllene (8.06%). Fourteen compounds were owned in both leaves and fruits. Conclusion: The main volatile compounds from leaves of L. glauca are obviously different from fruits.关键词：Lindera glauca;volatile compounds;solid-phase microextration;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the bud of Trachycarpus fortunei. Method: The constituents were isolated and purified by using macroporous resin column chromatography, silica gel column chromatography and Sephadex LH-20 chromatography, their structures were identified by physicochemical properties and spectrum analysis. Result: Eight compounds were separated and six of them were isolated and identified as isovanillic acid (Ⅰ), β-sitosterol (Ⅱ), dioscin (Ⅲ), 5-O-caffeoylshikimic acid (Ⅳ), caffeic acid (Ⅴ), rutin (Ⅵ). Conclusion: Compounds Ⅰ, Ⅱ, Ⅳ, Ⅴ and Ⅵ are firstly obtained from T. fortune, compounds Ⅰ and Ⅳ are firstly obtained from the plant of Trachycarpus.关键词：bud of Trachycarpus fortune;chemical constituents;5-O-caffeoylshikimic acid
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发布时间：2024-01-04 - 摘要：Objective: To study antimicrobial acitvity of Aquilaria sinensis peel extract. Method: The chloroform extract of A. sinensis peel was investigated for antimicrobial activity against the tested bacteria and fungi using filterpaper disc method and growth rate method respectively and their minimum inhibitory concentrations (MICs) were determined using serial dilution method. Result: The extract displayed significant antibacterial and antifungal activities against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa with MIC values of 6.25,6.25,12.5 g ·L-1 and against Trichoderma roseum, Aspergillus niger and A.flavus with MIC values of 6.25,6.25,12.5 g ·L-1. Conclusion: The chloroform extract of A. sinensis peel exhibited antimicrobial activity which is worthy of exploitation.关键词：Aquilaria sinensis peel;extract;antimicrobial activity;minimum inhibitory concentration
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发布时间：2024-01-04 - 摘要：Objective: Changes of chlorophyll, soluble sugar, soluble protein, during the plant regeneration in Rabdosia rubescens werestudied to explore the correlation between the accumulation of the active components and physiological characteristics of cultural materials of R. rubescens. Method: UV spectrophotometry and high performance liquid chromatography were used to determine the content of chlorophyll, soluble sugar, soluble protein in the cultural materials of different stage. Result: The content of chlorophyll, soluble protein and acetic acid was positively correlated with the oridonin, while the soluble sugar had a negative correlation with the oridonin. Conclusion: The content of chlorophyll, soluble protein in the cultural materials had a mutual promotion relationship with the oridonin, and the levels of soluble sugar were closely related to the growth speed and developmental phase of the cultural materials.关键词：Rabdosia rubescens;callus;Oridonin
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发布时间：2024-01-04 - 摘要：Objective: The characterization on growth and some secondary metabolites of Artemisia annua hairy roots were investigated. Method: We investigated the effects of plant growth regulators such as NAA and GA3 on the hairy roots and untransformed roots. The main components of some secondary metabolites were tested in the hairy roots. Result: NAA at lower concentration (0.01-0.05 mg ·L-1) could promote formation of lateral and branch roots and had no effect on untransformed roots. High concentrated NAA (0.1 mg ·L-1) inhibited the growth of hairy roots but stimulated the growth of untransformed roots. Artemisinin was not detected in untransformed roots, but at 0.64 mg ·g-1 in hairy roots. To the flavonoids in A. annua, chrysoplenetin was found in both roots, but there was a higher content of arteannuin (0.76 mg ·g-1) in hairy roots, 3.5 folds of that in untransformed roots. Conclusion: A. annua hairy roots have a unique characterization on growth and secondary metabolites.关键词：Artemisia annua;hairy root;growth;secondary metabolites;untransformed roots
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发布时间：2024-01-04 - 摘要：Objective: To study on the microscopic and UV-Vis spectrum character of Cardiospermum halicacabum,a Zhuang folk medical herb,which is popularly applyed in Guangxi Zhuang Autonomous Region. Method: The microscopic identification and UV-Vis spectrum identification were adopted. Result: The fibers formed into interval circularity in the cortex of the root. The fibers showed ring-shaped in the cortex of the stem. There were two vascular bundles in main vein of the leaves. Non-glandular hairs and glandular hairs were found in the leaf surface. It could be observed that two kinds of fibers, lots of non-glandular hairs, glandular hairs, calcium oxalate cluster crystals and square crystals were in the powder. Six kinds of solvent of crude drug had absorption peaks in 200-800 nm, respectively. Conclusion: These results as above can be used to provide the scientific evidence for the establishment of standard for quality controland further study.关键词：Cardiospermum halicacabum;microscopic identification;UV-Vis spectrum identification
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Complex Bajitian mixture on the expression of VEGF mRNA in SD rats with steroid-induced osteonecrosis of the femoral head. Method: Seventy-two adult SD rats were randomized into four groups: normal control group, model group, positive control group and therapeutic group. Rat model of osteonecrosis of femoral head was established by injecting glucocorticoid. Each group was given drugs by gastric infusion one time a day. The rats were killed in the fourth, eighth and twelfth week respectively. The expression of VEGF mRNA was evaluated by RT-PCR. Result: The expression of VEGF mRNA was increased significantly after treatment on the 12th week compared to the 4th and 8th week (P<0.01). Conclusion: Complex Bajitian mixture can up-regulate the expression of VEGF mRNA. The therapeutic effect is close with the Xianling Gubao capsule.关键词：Complex Bajitian mixture;steroid-induced osteonecrosis of the femoral head;experimental research;vascular endothelial grouth factor
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of Eucommia ulmoides agains UV irradiation-induced damage in ESF-1 cells and its mechanism. Method: E. ulmoides was extracted by 70 ethanol, then the extract was eluted with gradient of ethanol by macroporous resin,ESF-1 cells were immediately treated with different concentrations of 50% ethanol eluate after UVA irradiation (40 J ·cm-2) or UVB irradiation (70 mJ ·cm-2). Twenty-four hours later, the proliferation of cells was detected by MTT, SOD, LDH and MDA content were assayed by colrorimetry and TBA methods respectively. Result: Compared with the UVA group, the concentration of 125,250 mg ·L-1 could increase the proliferation(P<0.05) and reduce the activity of LDH (P<0.05). The concentration of 250 mg ·L-1 could increase the activitiy of SOD (P<0.05) and decreased the content of MDA (P<0.05). Compared with the UVB group, the concentration of 125, 250 and 500 mg ·L-1could increase the proliferation (P<0.05). The concentration of 125 mg ·L-1could increase the activity of SOD (P<0.05) and reduce the activity of LDH (P<0.05) and decreased the content of MDA (P<0.05). Conclusion: The ethanol eluate of 50% can inhibit UV irradiation-induced damage in ESF-1 cells. Its mechanism is due to its abilities of increasing SOD, scavenging oxygen free radicals, inhibiting lipid peroxidation, and protecting membrane structure.关键词：Eucommia ulmoides;ultraviolet;photoaging;ESF-1 cell
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发布时间：2024-01-04 - 摘要：Objective: This paper studies Chinese herbal compound of Huanglian Jiedu decoction and its extract systems by gradient extraction with three kinds of organic solvents and the changes in the indicative components in different polarity parts. Through pharmacodynamics screening, utility location and chemical composition which can improve cerebral ischemia and promote noopsychic function were approached, to provide the basis for refining separation. Method: Huanglian Jiedu decoction was extracted successively with water (partⅠ), ethyl acetate, water saturated butanol, the petroleum ether extract (partⅡ), ethyl acetate extract (partⅢ), butanol extract water saturated butanol extract (partⅣ), supernatant (partⅤ) and sediment (partⅥ) of residual water layer extract. Using RP-HPLC, and geniposide, baicalin, palmatine, berberine, baicalein and wogonin as the indicative components, each component in every extract system was detected. Finally, using PC12 cells, low-sugar of DMEM and cobalt chloride to made oxygen and glucose deprivation model, MTT was applied to detect the cell viability. Result: The quality of the indicative components in partⅤwas higher, having the similar ratio with partⅠ; partⅠand partⅤhad the highest protective effect in the oxygen and glucose deprivation injured cells. Conclusion: PartⅤ is the effective fractions that can improve cerebral ischemia and noopsychic function.关键词：Huanglian Jiedu Decoction;gradient extraction;polarity parts;pharmacodynamic screening
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发布时间：2024-01-04 - 摘要：Objective: To investigate the hepatoprotective effects of Cichorium glandulosum extracts (CG-Ⅰand CG-Ⅱ) against alcohol-induced acute liver injury in mice. Method: The acute liver injury animal model was established in mice by alcohol gavage (35% at 16 mL ·kg-1). The liver and spleen indexes, alanine aminotransferase (ALT), activities of serumaspartate amiontransferase (AST), cholesterol (CHO), triglycerides(TG), activities of superoxidedismutase (SOD) and lutathione peroxidase(GSH-Px), contents of malondialdehyde (MDA) and nitric oxide (NO) in liver homogenate, as well as the histopathological changes were all studied. Result: CG-I and CG-Ⅱsignificantly reduced the spleenindex and activities of serum ALT and TG, increased the activities of liver SOD and GSH-Px, lowered the contents of MDA and NO (P<0.05 or P<0.01). Specifically, CG-I (1.0,2.0 g ·kg-1) and CG-Ⅱ(1.2,2.4 g ·kg-1) decreased the liver index (P<0.05); CG-I(1.0 g ·kg-1) and CG-Ⅱ(1.2 g ·kg-1) decreased the activity of serum AST (P<0.05); CG-Ⅰ(1.0 g ·kg-1) and CG-Ⅱ(2.4 g ·kg-1) decreased the activity of serum CHO (P<0.05 or P<0.01); CG-Ⅰand CG-Ⅱ improved liver injuries. Conclusion: CG-I and CG-Ⅱ showed protective effects against acute liver injury induced by alcohol in mice.关键词：Cichorium glandulosum;alcoho-induced liver injury;mice
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Ginseng Two-ingredient Capsule on blood glucose and blood lipids in the diabetic rats. Method: Sixty SPF male Wistar rats with the weight of (180±20) g were given normal forage adaptively for 7 days. Ten rats were selected randomly to form normal group. With high fat forage diet of four weeks, the rest 50 rats were injected with 30 mg ·kg-1 of STZ intraperitioneally to establish rat model of diabetes. The model rats were randomly divided into model control group with 10 rats, Ginseng Two-ingredient Capsule group with 10 rats ig given the capsule, metformin hydrochloride group with 10 rats served metformin hydrochloride by way of ig, and Ginseng-Astragalus Hypoglycemic Granule group with 10 rats treated with Ginseng-Astragalus Hypoglycemic Granule by way of ig for 14 days. The fasting blood glucose and weight of the rats were measured before the treatments and after the treatments for two weeks. Blood sample was finally obtained from aorta of rats. Triglyceride and cholesterol were tested to evaluate the influence of Ginseng Two-ingredient Capsule on blood glucose, blood lipids, and the body weight in the diabetic rats was also investigated. Result: Fourteen days later after medicine treatment, Ginseng Two-ingredient Capsule could reduce blood sugar and blood lipids in the type 2 diabetic rats and control weight-reduction for rats effectively which have significant difference compared with that in the model group (P<0.05). Conclusion: Ginseng Two-ingredient Capsule can reduce blood sugar and blood lipids in type 2 diabetic rats and control weight-reduction for the rats effectively. However, its hypoglycemic effect was inferior to that of metformin or Ginseng-Astragalus hypoglycemic Granule.关键词：Ginseng Two-ingredient Capsule;Hypoglycemic effect;Wistar rat;hypolipidemic effect
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Anshen Dingzhi Ling (ADL) on the expression of dopamine receptor-1 (DRD1) and dopamine receptor-2 (DRD2) in prefrontal cortex and striatum of spontaneously hypertensive rat (SHR) model of attention deficit hyperactivity disorder (ADHD), and to investigate the mechanisim of ADL treatment for ADHD. Method: Thirty SHR rats were randomly divided into 5 groups: untreated model group, ritalin group (2.1 mg ·kg-1 by gavage), high dose of ADL group, middle dose of ADL group and low dose of ADL group (ig ADL with the crude drug dosage 34.1, 17.1 and 8.5 g ·kg-1 respectively). The normal control group includes 5 Wistar rats (given normal saline 10 mL ·kg-1 by gavage). The rats were killed after 14 days of treatment, then the expression of DRD1, DRD2 mRNA and protein in the prefrontal cortex and striatum of rat brain were detected by RT-PCR and Western blot. Result: Compared with normal group, the levels of DRD1, DRD2 mRNA and protein in model rats reduced significantly (P<0.01); the levels of DRD1, DRD2 mRNA and protein of ritalin group was significantly higher than that in the model group (P<0.01); the levels of DRD1, DRD2 mRNA and protein of the middle dose group was significantly higher than that in the model group (P<0.01);the levels of DRD1, DRD2 mRNA and protein of ADL low dose group and high dose group were higher than that in the model group, but there is no significant difference. Conclusion: ADL can up-regulate the expression of DRD1, DRD2 mRNA and protein in the prefrontal cortex and striatum in SHR, may be related with the influence to the activity of adenylyl cyclase. It shows that ADL plays an important role on regulation to the prefrontal-striatal pathway, and DRD1, DRD2 involved in the adjustment process, the ADL eventually has therapeutic effect for the treatment of ADHD.关键词：Anshen Dingzhi Ling;attention deficit hyperactivity disorder,;spontaneously hypertensive rat;dopamine receptor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the action mechanism of Danshen injection on red blood cell deformability in aged rats. And the change in hemorheological indexes and cholesterol in erythrocyte membrane was studied. Method: Thirty Wistar rats (70-72 weeks old) were randomly assigned to Danshen injection high-dose group (4.4 g ·kg-1), low-dose group (1.1 g ·kg-1) and model group with 10 rats in each group. Another ten younger Wistar rats were as young control group. The related indexes of hemorheology, such as the whole blood viscosity, plasma viscosity and the red blood cell deformability, were determined while the contents of cholesterol in erythrocyte membrane were determined by HPLC after giving medicine 14 days by intraperitoneal injection. Result: Compared with the model group, the Danshen injection group was effective. The whole blood viscosity (P<0.01)、plasma viscosity (P<0.05) and the content of cholesterol in erythrocyte membrane (P<0.01) were decreased. And the deformability of erythrocyte (P<0.01) was increased. Conclusion: Danshen injection can decrease the content of cholesterol in erythrocyte membrane and affect the metabolism of lipid which maybe one of the mechanism of increasing deformability of erythrocyte and improving the blood viscosity.关键词：aged rats;Danshen injection;hemorheology;erythrocyte membrane;cholesterol
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Huayu Qutan recipe and its separated recipes on the expression of inflammatory factors in cultured human umbilical veins endothelial cells EA.hy 926 induced by LPS. Method: SD rats were equally divided into five groups in random: the control group, Huayu Qutan recipe group, Buqi recipe group, Huayu recipe group and Qutan recipe group. They were ig given daily with normal saline and recipes respectively. Blood drawn from abdominal aorta of the rats 2 h after ending of the treatments on the 9th day, and the serum was takenfor testing. EA.hy926 cells were cultured and equally divided into seven groups randomly: the normal control group, LPS stimulating group, Huayu Qutan recipe group, Buqi recipe group, Huayu recipe group, Qutan recipe group as well as normal serum control group. Then the mRNA levels of NF-κB and TNF-α were tested by RT-PCR. The protein levels of TNF-α and NF-κB were detected by ELISA technique and fluorescent immunocytochemistry method, respectively. Result: The expression of NF-κB and TNF-α were both increased compared to normal group after stimulation of LPS(P<0.01). The expression of NF-κB and TNF-α in Huayu Qutan recipe group and Huayu recipe group were obviously inhibited compared to that of LPS stimulating group (P<0.01). Conclusion: The molecular mechanism of Huayu Qutan recipe and Huayu recipe against atherosclerosis is probably related with the inhibition of expression of NF-κB and TNF-α.关键词：Huayu Qutan recipe;atherosclerosis;inflammation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the change of ALPase during the differentiation from rat marrow mesenchymal stem cells to osteoblasts induced by Zuogui Pill medicated serum. Method: The method of adherent culture was used to culture marrow mesenchymal stem cells and then consecutively to purify mesenchymal stem cells. In conventional induction group, β-glycerophosphate, dexamethasone and vitamin C was used to induce mesenchymal stem cell to differentiate into osteoblasts. In the experimental group, on the basis of conventional induction Zuogui Pill was applied to get the medicated serum. Alizarin red staining was used for showing calcium nodules and alkaline phosphatase staining was used to identify the successful induction. On the 0, 7th, 14th and 21st day a colorimetric assay of alkaline phosphatase was carried out respectively. Result: Whole bone marrow adherent method combined with differential adhesion method could get the purified mesenchymal stem cells. Alkaline phosphatase measured by colorimetry increased with the prolongation of induction, and the induction by the medicated serum was more significant. Conclousin:Rat mesenchymal stem cells could differentiate into osteoblasts when induced by drugs. Zuogui Pill medicated serum could promote the express of alkaline phosphatase.关键词：mesenchymal stem cells;Zuogui Pill medicated serum;osteoblasts;alkaline phosphatase
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Guben Yishen Capsule(GB-C)on the immune function in mice. Method: Mice were divided into five groups. By the effect of the indexes of spleen and thymus,the level of serum hemolysin antibody,carbon particle clearance swallowing index,delayed type hypersensitivity(DTH)reflect the immune function. Result: GB-C can enhance the indexes of spleen and thymus,increase the number of K and α,improve the level of serum hemolysin antibody and the number of DTH was increased. Conclusion: GB-C can enhance the non-specific immunity and specific immunity function in mice.关键词：Guben Yishen Capsule;non-specific immunity;specific immunity
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effects of the extracts from stems and leaves of Oplopanax elatus on the immune functions of immune-suppressed mice by cyclophosphamide. Method: Mice were randomly divided into 5 groups: control group, model group, low, moderate and high dose groups (1.3, 2.6, 5.2 g ·kg-1) of the extracts from stems and leaves of O. elatus. The mice were examined for immune organ weight index, carbon particle clearance capability of macrophage, delayed type hypersensitivity. Result: Compared with model group, all dose groups could significantly increase the weight of the immune organs, carbon particle clearance capability of macrophage and delayed type hypersensitivity(P<0.05-P<0.01). Conclusion: The extracts from stems and leaves of O. elatus can enhance the immune functions in immune-suppressed mice.关键词：stems and leaves of Oplopanax elatus;immune function;cyclophosphamide
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective action of Panax notoginseng saponins (PNS)on immunological liver injury in mice. Method: Mice were randomly divided into five groups, control group, model group, low-dose (100 mg ·kg-1) of PNS, middle-dose (200 mg ·kg-1) of PNS, high-dose (400 mg ·kg-1) of PNS and bifendate (150 mg ·kg-1) group. The model of mouse with immunological liver injury was induced by injection of BCG and LPS from tail vein. The ALT, AST, MDA content, SOD and GSH-Px activities were assayed by spectrophotometry, hepatic tissues stained with hematoxylin and eosin (HE) were prepared for histological analysis to liver injury. Moreover TNF-α mRNA expression in liver tissue was detected by RT-PCR technology. Result: PNS could decrease the enlarged liver, spleen index, ALT, AST and MDA content(P<0.05 or P<0.01), prevented the reduction of SOD and GSH-Px activities in liver homogenate(P<0.05 or P<0.01). Meanwhile, the pathological injury of hepatic tissues was alleviated and the expression of TNF-α mRNA in liver tissue was inhibited. Conclusion: PNS showed significant protective action on immunological liver injury in mice.关键词：saponins of Panax notognseng;immunological liver injury;bacille calmette guerin;lipopolysaccharide
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发布时间：2024-01-04 - 摘要：Objective: To investigate the renal protective effect of Curcuma wenyujin on the kidney in diabetic rats. Method: Fouty Wistar rats were divided into sham operation group, model group and C.wenyujin-treatment group. Nephrectomy and streptozocin(STZ,45 mg ·kg-1ip) were used to induce diabetic nephropathy and treated with C.wenyujin(3 g ·kg-1 ·d-1 ig).Blood glucose(Glu),total cholesterol(TC),serum creatinine clearance,amount of urinary albumin, connective tissue growth factor (CTGF) protein and pathologic change of kidney tissue were observed. Result: The amount of Glu, TC, urinary albumin and renal protein expression of CTGF were markedly increased in model group compared with those in normal group(P<0.01). Pathologic changes of kidney tissue presented further development. Treatment with C.wenyujin could significantly decrease the above indexes compared with those in diabetic model group(P<0.01),and pathologic changes of kidney tissue were significantly improved. Conclusion: C.wenyujin has reno-protective effects on diabetic nephropathy in rats, and these effects may be correlated with its inhibition on over-expression of CTGF in kidney.关键词：Curcuma wenyujin;diabetic nephropathy;connective tissue growth factor
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发布时间：2024-01-04 - 摘要：Objective: To study the effect extracts from Xinjiang Coriopsis ticntoria flowers (CTF) on α-glucosidase activity. Method: Five extracts of CTF were prepared by water extraction and organic solvent abstraction. Including CTF ethylacetate extraction, CTF normal butyl alcohol extraction, CTF total flavanone, CTF water extraction and CTF neutral flavanone. The inhibitory effects of these extracts to α-glucosidase activity were investigated by in vitro enzymatic method; and akarbose was used as positive reference substance. Result: The extracts of CTF showed obviously inhibitory action against α-glucosidase activity in which 4 extracts have higher effects compared to those of acarbose, among them, the extract of CTF neutral flavanone was more effective for enzyme inhibition with a inhibitory rate of 87.26%. The CTF IC50 of the five extracts were: Acarbose IC50 858.0 mg ·L-1, CTF ethylacetate extraction IC50 12.5 mg ·L-1, CTF normal butyl alcohol extraction IC50 139.5 mg ·L-1, CTF total flavanone IC50 163.5 mg ·L-1, CTF water extraction IC50 367.6 mg ·L-1, CTF neutral flavanone IC50 5.8 mg ·L-1. Conclusion: Coriopsis ticntoria flowers extracts had remarkable inhibitory effect to α-glucosidase activity. This finding provides important information for pharmacological screening of new anti-diabetic agent from CTF plant.关键词：Xinjiang Coriopsis ticntoria flowers extract;α-glucosidase;inhibitory activity
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of tetramethylpyrazine injection on the angiogenesis and expression of vascular endothelial growth factor(VEGF)mRNA in ischemic myocardium of the rats with myocardial infarction(MI). Method :The left coronary artery of male Wistar rat was ligated to make the model of acute myocardial infarction.All rats were randomly divided into Tetramethylpyrazine injection group,Shexiang Baoxin Pills group(positive control group),model group and sham operation group. Microvasscular vascular density(MVD) and VEGF mRNA in ischemic myocardium of rats with MI were detected six weeks later. Result :MVC and MVD in ischemic myocardium of rats with MI in modle group, tetramethylpyrazine injection group and Shexiang Baoxin Pills group were higher than that in sham operation group.In tetramethylpyrazine injection group they were the same as Shexiang Baoxin Pills group,which were higher than that in modle group.The expression of VEGFmRNA in modle group were elevated compared with that in sham operation group.It was significantly elevated in tetramethylpyrazine injection group compared with that in modle group and lower than that in Shexiang Baoxin Pills group. Conclusion :tetramethylpyrazine injection may promote angiogenesis in ischemic myocardium of the rats with MI.One of the mechanisms might be that tetramethylpyrazine injection could promot the expression of VEGFmRNA in ischemic myocardium of the rats with MI.关键词：tetramethylpyrazine injection;myocardium infarction;angiogenesis;VEGFmRNA
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发布时间：2024-01-04 - 摘要：Objective: To study the interventive affect and mechanism of Buyang Huanwu decoction (BYHW decoction) on von Willebrand factor (vWF) of animal with metabolic syndrome(MetS). Method: Rats were distributed randomly into 4 groups: empty control, model control, BYHW decoction group,BYHW decoction without Astragali Radix group. To set up pathological modal of MetS, rats were given streptozotocin (STZ) and feed with high-fat and high-carbohydrate diet. Dose: BYHW decoction group 7.02 g ·kg-1 ig,BYHW decoction without Astragali Radix group 1.07 g ·kg-1 ig. Both were once a day. Experiment lasts for 8 weeks. Fasting blood glucose (FBG), insulin(Ins), homeostasis model assessment-insulin resistance(HOMA-IR) and vWF in serum were observed. Result: Compared with blank control, Ins, HOMA-IR and vWF were higher in model group (P<0.05). Compared with model, BYHW decoction group was lower in Ins, HOMA-IR and vWF(P<0.05); BYHW decoction without Astragali Radix group was higher in FBG (P<0.01), no evident differences in Ins, HOMA-IR, and lower in vWF. Conclusion: BYHW decoction might prevent IR and reduce vWF. Astragali Radix is the effective part in preventing IR and six other drugs may reduce vWF.关键词：Buyang Huanwu Decoction;metabolic syndrome;von willebrand factor
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发布时间：2024-01-04 - 摘要：Objective: To compare the effective similarities and differences between the Xiaoqinglong decoction and Mahuang Fuzi Xixin decoction on experimental allergic rhinitis (EAR) in guinea pigs. Method: EAR was induced in guinea pigs with ovum albumin, and all the animals with EAR are randomly divided into 4 groups, i.e. EAR model group, the Xinqin granules control group treating with Xinqin granules 4.65 g ·kg-1, treatment group with the Xiaoqinglong decoction 7.44 g ·kg-1,and treatment group with the Mahuang Fuzi Xixin decoction 2.33 g ·kg-1, supplemented with a normal group as the control. The experiment lasted 14 days. During the experimental period, attention was paid to observe changes in symptoms, histamine content in blood and morphological features of nasal mucosa under light microscopy. Result: The three treatment groups all reduced the accumulative scores of the symptoms and histamine content in blood, and showd highly signifieant compared with that of the normal group (P<0.01). The treatment group with the Mahuang Fuzi Xixin decoction showd highly signifieant(P<0.01)in reducing histamine content in blood(3.64±0.83) mg ·L-1,compared with the Xiaoqinglong decoction group(4.58±0.51) mg ·L-1 and the Xinqin granules group(4.36±0.13) mg ·L-1, and also acted better on recovering the nasal mucosa. Conclusion: Mahuang Fuzi Xixin decoction is more effective in treaing EAR, and it acts better than the Xiaoqinglong decoction on ruducing histamine content in blood and recovering the nasal mucosa.关键词：allergic rhinitis;guinea pig model;Xiaoqinglong decoction;Mahuang Fuzi Xixin decoction
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of the Shenshuai Baoshen Capsule on extracellular matrix accumulation in rat renal interstitial fibroblast, and analyze the mechanisms of the kidney protection effects of the Shenshuai Baoshen Capsule. Method: Sixty Wistar rats were randomly divided into blank control group, model control group, Shenshuai Baoshen capsule low dose group and high dose group. The model was established by ig administration of adenine. Different groups were treated for 8 weeks,The pathlological changes in kidney functions after therapy were observed. The protein collagen Ⅰ, MMP-1(matrix metalloproteinase-1), TIMP-1(tissue inhibitor of metalloproteinase-1), PAI-1(plasminogen activator inhibitor) expression in kidney tissue were detected respectively by immunohistochemistry. Result: The pathlological changes were reduced in Shenshuai Baoshen capsule group, Cr, BUN and UA were reduced, the expression of collagen Ⅰ, TIMP-1,PAI-1 protein in kidney tissue were reduced, and the expression of MMP-1 was induced which had the significant difference compared with that in the model group(P<0.01,P<0.05). Conclusion: The Shenshuai Baoshen Capsule could depress the collagen Ⅰprotein accumulation in renal tissue, by rising MMP-1 expression, depressing TIMP-1,PAI-1 protein expression. Therefore, the renal interstitial fibroblast was relieving by Shenshuai Baoshen Capsule, and chronic renal failure progress was delayed.关键词：renal interstitial fibroblast;matrix metalloproteinase-1;tissue inhibitor of metalloproteinase-1;plasminogen activator inhibitor;Shenshuai Baoshen Capsule
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发布时间：2024-01-04 - 摘要：Objective: The experimental study aims to study the pharmacodynamics of Xiangyan Zhitong decoction in primary dysmenorrheal therapy, we observed the pain-releasing effects of Xiangyan Zhitong decoction on dysmenorrheal in mouse and rat models induced by oxytocin. Changes of PGE2 and PGF2α content in rat peripheral blood and uterus tissues were measured, which could provide the pharmacological basis for clinical application of Xiangyan Zhitong decoction. Method: Dysmenorrheal was induced by diethylstilbestrol and oxytocin in mouse and rat with aspirin as the control, and the effectss of Xiangyan Zhitong decoction were observed for releasing oxytocin-induced pain and on PGE2 and PGF2α expression in rat peripheral blood and uterus tissue. Result: Xiangyan Zhitong decoction significantly inhibited the pain reactions in mice and rats induced by oxytocin. The content of PGE2 increased obviously in rat peripheral blood and uterus tissue, while PGF2αdecreased significantly. TCM group showed better effectiveness.Conclusions: Xiangyan Zhitong decoction could raise pain threshold and change the secretion of PGE2 and PGF2α. It has anti-dysmenorrheal effects.关键词：Xiangyan Zhitong decoction;primary dysmenorrheal;Chinese medicine therapy;pharmacodynamics
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发布时间：2024-01-04 - 摘要：Objective: To investigate the wound healing effect of Jintangbao Spray for promoting superficial second degrees burn, as well as anti-inflammatory and analgesic actions. Method: The effect of wound healing was investigated through superficial second degrees burn model in rats and feet burn model in mice. Anti-inflammatory effect was investigated through carrageenan-induced swelling of rat toes. Analgesic effect was investigated through formaldehyde-induced pain in mice. Result: Compared with the model group, Jintangbao Spray could remarkly promote healing of cutis in the trauma with superficial second degrees burn in rats. The pathomorphological evidences of hepatic tissue showed thatJintangbao Spray could remarkably repair cutaneous epidermis injury and the disorganized collagen fiber-bundle of dermis, and promote dermal follicle proliferation, as well as lighten inflammatory cell intiltration. It could significantly reduce water content in the feet burn model in mice, inhibit the carrageenan-induced swelling of rat toes and lighten the pain caused by formaldehyde in mice. Conclusion: Jintangbao Spray can remarkly promote healing of cutis in the trauma and has anti-inflammatory and analgesic effects, and the evidences provide the basis for clinical application of the medicine.关键词：Jintangbao Spray;superficial second degrees burn;anti-inflammatory effect;analgesic effect
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发布时间：2024-01-04 - 摘要：Objective: To investigate the relaxative characteristics of paeoniflorin on thoracic aortic artery in rat and its mechanism. Method: We perfused the isolated rings and observed the response of NA-induced artery contraction to paeoniflorin under the Ca2+-contained and Ca2+-free bath solutions. In the same way the effects of paeoniflorin on the vascular smooth muscle were observed by adding KCl(60 mmol ·L-1),and the effect on the contraction of the vascular smooth muscle depending on the intrace11ular calcium and extracellular calcium were also observed by adding NA. We also observed the effect of paeoniflorin on the contraction of rings induced by NA in the presence of L-NAME. Result: Peoniflorin relaxed rat aorta rings precontracted by NA in a dose-dependent manner.But had no effect on the aorta’s contraction induced by intracellular calcium and extracellular calcium as well as KCL(60 mmol ·L-1),the relaxant effect could be attenuated by L-NAME.There was significant change in the group of paeoniflorin(P<0.05). Conclusion: The results indicate that paeoniflorin relaxes vascular smooth muscle in an endothelium-dependent manner. The mechanisms seem to be related with promoting synthesis and release of NO,but not for opening Ca2+ activated K+ channel as well as the inhibition of Ca2+ influx and release of Ca2+ from intracellular stores.关键词：paeoniflorin;thoracic aortic ring;mechanism
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发布时间：2024-01-04 - 摘要：Objective: Analgesic,anti-inflammatory and anti-ulcer were choosed as pharmacological targets to evaluate the pharmacodynamics of Yulian Cataplasm therefore to provide the experimental basis for clinical applications of the cataplasm. Method: The rat experimental gastric ulcer was established by using pyloric ligation. Rat paw edema was caused by inflammation action of carragenan. The mouse model of analgesia was established by the method of somatic wrenching. The effects of Yulian Cataptosm(rats 12,6,3 g ·kg-1, mice 6,3,1.5 g ·kg-1,ig)were evaluated by ulcer index, inhibition rate and the incidence of ulcers, foot swelling, writhing and capillary permeability. Yulian cataplasm of high,middle and low dose groups were used respectively. Result: The external Yulian Cataplasm lowered ulcer index in a dose dependent manner. only the high and middle dose groups could increase excretion of pepsin while reduced the incidence of ulcers. Both middle and low dose groups could reduce rat paw swelling, middle dose group lasted longer duration of action. High and middle dose groups could decrease capillary permeability, all the three dose groups could reduce the writhing, and compared with excipient group, there was significant difference (P<0.05, P<0.01). Conclusion: Yulian Cataplasm could obviously inhibite the gastric ulcer, pain and swelling with the role of anti-inflammatory, it also provided an experimental basis on the feasibility of treating gastrointestinal diseases with transdermal drug delivery.关键词：Yulian Cataplasm;peptic culcer;transdermal delivery system
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Xifeng Jingning Tang on immunologic function in mice with immunodepression. Method: The immunodepression mice model was induced by intraperitoneal injection of cyclophosphamide with 50 mg ·kg-1. Mice were administrated with Xifeng Jingning Tang at doses of 3,6,12 g ·kg-1 for 7 days.The phagocytic activity of monocytes, the delayed hypersensitivity reaction and the haemolysin antibody formation in mice were observed by means of carbon particle clearance,ear swelling and haemolysin antibodies respectively. Result: The doses of 6 and 12 g ·kg-1 ·d of Xifeng Jingning Tang could improve immune function in mice shown by carbon clearance index, phagocytic index and ear swelling. Xifeng Jingning Tang 12 g ·kg-1 could increase content of haemolysin antibody induced by cyclophosphamide. Conclusion: Xifeng Jingning Tang can improve non-specific immunity,and enhance specific cellular immunity and humoral immune function in immunodepression mice induced by cyclophosphamide.关键词：Xifeng Jingning Tang;immune function;cyclophosphamide;Tourette syndrome
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Danggui San on uterine smooth muscle in rats and the related mechanism of it. Method: The effect of Danggui San decoction on the spontaneous activity of uterine smooth muscle by was observed PowerLab 10T biological signaling system and the activity of isolated uterus smooth muscle was recorded. The indexes include the amplitude, frequency / period, baseline and mobility in rat’s uterine smooth muscle movement track. Result: The water extracts of Danggui San could significantly restrain the amplitude,baseline and mobility; oxytocin could significantly drive up the baseline and increase the frequency. Danggui San could rectify these changes back to the normal level. Conclusion: The water extracts of Danggui San can restrain the mobility of uterine smooth muscle and rectify the excitation of oxytocin. This can afford impersonal evidence for illustrating Danggui San for stabilizing embryo and the partial mechanism involved.关键词：Danggui San;rat;uterine smooth muscle
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发布时间：2024-01-04 - 摘要：Objective: To investigate the methods in copying the animal proliferative breast model. Method : We copied the animal proliferative breast model with different experimental programs,according to the theory of homogeneous design. And we evaluated varying degrees of success of these models with such indexes as the diameter of the mammilla, height of the mammilla and pathological dye of the galactophore. Result : Compared with the blank control group, the experimental group of animals’ nipple diameter and height were significantly increased in the breast tissue hyperplasia of mammary glands are typical, with the fifth, sixth group was the most obvious. Conclusion: The optimal conditions for establishing the model appear to be:estradiol benzoate 0.42-0.5mg ·kg-1, the duration for 25-35 days and progesterone 3-4.5 mg ·kg-1, intramuscular injection for 2-3 days.关键词：hyperplasia of mammary gland;animal model;method to establish model;homogeneous design
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of Dihuangyinzi on learning and memory and AChE,ChAT of Hippocampal in chronic cerebral hypoperfusion rats. Method: Using the model of chronic cerebral ischemia by two-vessel occlusion, Learning and memory memory improvement was measured by Morris water maze test in rats of chronic cerebral hypoperfusion of Dihuangyinzi, and AChE,ChAT content were measured in hippocampus of rats. Result: Compared with the control group,Morris water maze test,Dihuangyinzi group escape latency Shortened,the number of cross platform increased,AChE and ChAT Content in the hippocampus were significantly increased. Conclusion: Dihuangyinzi can improve learning and memory function in rats of chronic cerebral hypoperfusion,which may protect the cholinergic system.关键词：Dihuangyinzi;chronic cerebral hypoperfusion;AChE;ChAT
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of RG-14260 on the proliferation and drug sensitivity of human endometrial carcinoma line ishikawa cells in vitro. Method: Cell proliferation inhibition rate of ishikawa cells was determined using MTT method after treatment with half of the IC50of the ADR,DDP,PTX and 10 μmol ·L-1RG alone or combination for 24 h. The mRNA levels of EGFR after treatment with RG and drug alone was detected by RT-PCR. Western blot was used to measured p21 protein abundance after treatment with 10 μmol ·L-1RG for 6,12,24 h. Result: RG-14260 significantly decreased the mRNA expressions of EGFR and increased the abundance of p21 protein. Compared with the treatment with agents alone, combined treatment of the RG-14260 significantly increased cell proliferation inhibition rate and improved the cytotoxicity of the chemotherapeutic agents(P<0.05). Conclusion: RG-14260 can block the EGFR pathway and increase p21 protein to inhibit the proliferation and enhance the chemosensitivity of human endometrial carcinoma ishikawa cells.关键词：endometrial carcinoma;RG-14260;epidermal growth factor receptor(EGFR);p21
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发布时间：2024-01-04 - 摘要：Objective: To study the inhibitory effect and mechanism of phenylpropanoid glycosides from Boschniakia rossica (PGBR) on proliferation of lung carcinoma cells. Method: MTT assay was used to examine the proliferation of A549 cells, flow cytometry was used for the determination of cell cycle distribution and cell apoptosis, TUNEL method was used for the determination of cell apoptosis, and immunohistochemical method was used for the determination of p53, Fas, Bax and Bcl-2 protein expression. Result: PGBR inhibited the proliferation of lung carcinoma cells in a time-and concentration-dependent manner, the inhibitory ratios of 200 mg ·L-1 PGBR at 12, 24 and 48 h were(20.4±2.1)%,(27.1±2.0)% and (30.7±2.2)%, respectively. PGBR increased the percentage of G0/G1 phase cells from(45.3±4.1)% to (57.4±4.0)%, and increased the apoptotic ratio from(2.4±0.90)% to (7.8±1.2)% to (57.4±4.0)%, and increased the apoptotic ratio from(2.4±0.90)% to(7.8±1.2)%. Furthermore, PGBR increased the expression of p53, Fas and Bax proteins and decreased the expression of Bcl-2. Conclusion: PGBR could exert an anti-lung cancer effect via induction of cell cycle arrest and cell apoptosis.关键词：Boschniakia rossica;phenylpropanoids;lung cancer;proliferation;apoptosis;cell cycle
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发布时间：2024-01-04 - 摘要：Objective: To discuss the relationship between the liver fatty acid binding protein and kidney damage induced by aristolochic acid in rats evaluated by perfusion of isolated kidney. Method: Rat perfusion model of isolated kidney was established at the constant flow of 5 mL ·min-1.The control group was administrated with normal perfusate,while the experimental group was given aristolochic acid(7 mg in 50 mL).After 10 min of perfusion, the perfusate and urine at different time points were collected.Then concentration of K,Na,Cl was determined and their clearance was calculated.The content of liver fatty acid binding protein was detected by Elisa. Result: Compared with the control group,the clearance of K and Na in the experimental group given aristolochic acid began to decrease from (99.54±14.55)% and (27.93±6.66)% of pre-perfusion to (33.16±9.59)% and (9.70±2.62)% of 30 min after perfusion respectively,and liver fatty acid binding protein immediately increased significantly from (7.42±0.12)ng ·L-1 of pre-perfusion to (8.18±0.47)ng ·L-1 at the moment of after perfusion respectively. Conclusion: Liver fatty acid binding protein is superior to the clearance of traditional indicators in the evaluation of acute tubular injury induced by aristolochic acid.It is an early and sensitive biomarker of renal tubular injury.关键词：aristolochic acid nephropathy;perfusion of rat isolated kidney;liver fatty acid binding protein
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发布时间：2024-01-04 - 摘要：Objective: To study the distribution of isorhynchophyline in liver and kidney tissues of SHR rats before and after compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Unics Le, to investigate the impact of channel tropism before and after combination. Method: Uncariae Ramulus Cum Uncis decoction group was administrated Uncariae Ramulus Cum Unics,Tianma Gouteng decoction group was administrated with decoction of compatible Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis at a dose of 1.172 g ·kg-1. A blank group was received normal saline. Then using HPLC to determinate the isorhynchophyline in liver and kidney tissues of SHR rats before and after the compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis. Analytical column was WatersTM(4.6 mm×250 mm, RP 18.5 μm). The mobile phase consisted of methanol-water (70 ∶30),pH 8.0,rate was 10.8 mL · min-1. The detector wave length was set at 254 nm,the column temperature was 30 ℃. Result: Isorhynchophyline in liver and kidney tissues was linear in the range of 0.08-16.0 mg ·L-1. There existed significant (P<0.05) differences in liver and kidney tissues before and after the compatibility. Conclution :The compatibility of Chinese medicine has close correlation with channel tropism.关键词：Unicariae Ramulus Cum Uncis;Gastrodiae Rhizoma;compatibility;channel tropism;isorhynchophyline;distribution
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for analyzing isofraxidin in rat blood serum and investigate pharmacokinetic indexes of isofraxidin after oral administration of Yinxieling or Sarcandra glabra(Thumb.) Nakai. Method: Sprague-Dawley rats were administrated Yinxieling or decoction of S.glabra respectively. Serum concentration of isofarxidin was measured by HPLC. The results were analyzed by pharmacokinetic software DAS(2.0). Result: The linearity of the calibration curve was good in the range of 0.25-12 mg ·L-1 for isofraxidin and the detection limit for isofraxidin were 0.25 mg ·L-1.The extraction recoveries of isofraxidin were 70.9%-71.9% with RSD was 1.1%. The intra-day precision and inter-day precision of isofraxidin were lower than 8.9%. The difference of pharmacokinetic parameters between the group of Yinxieling and S.glabra were unremarkable. Conclusion: The method is exclusive,sensitive and accurate. Compound prescription of Chinese medicine did not affect the pharmacokinetics of isofraxidin.关键词：Yinxieling;Sarcandra glabra;isofraxidin;pharmacokinetics
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发布时间：2024-01-04 - 摘要：Objective: Observation of clinical curation effects on chronic obstructive pulmonary diseases of integrated traditional chinese and western medicine use Jibei by Kechuantang which has the efficacy of releasing lung and clearing heat, reducing phlegm and resolving masses. Method: Jibei Kechuan Tang which is made up of Reineckea carnea, Piloselloides hirsuta, Scutellaria baicalensis, Frifllaria thuubergii , Meretrix meretrix, Ephedra sinica, Radix Morus Alba, Lepidium apetalum, Bambusa txilis, Banbusa-lxilis, Pheretima aspergillum. Sixty two patients is of the treated group, 43 cases of control group received Kechuanshunwan. Two group besides on acute attack stage and infection of serious combining western medicine conventional anti-inflammatory treatment. Did not use relieving cough resolving phlegm and western medicine. Result: Treatment groups efficient 93.55%,control group efficient 81.40%。It was found on analysis of Ridit to contain the treatment group overall outperform the control group (P<0.05). Conclusion: Jibei Kechuantang to be able to improve the symptoms and signs, significantly of chronic obstructive pulmonary diseases, reduced the course to improve cure rates, reduce application of western medicine, reduce medical costs.关键词：Jibei Kechuantang;chronic obstructive pulmonary diseases(COPD)
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发布时间：2024-01-04 - 摘要：Objective: To improve the dosage form of Xinshenger Tuihuang mixture (XTM) and to change the administration route, and to observe its curative effect on neonatal jaundice. Method: Water extraction and alcohol precipitation preparation for improving XTM were used, lotion and enteroclyster were prepared. Patients conforming to inclusion criteria were randomly divided into observation group A, B and control group( n ＝60 each). Control group was given oral dose of phenobarbital sodium 8 mg ·kg-1,tid. for 4 d; observation group A was given oral dose of XTM 10 mL,tid. for 4 d;and observation group B was given Chinese materia medica as enteroclysm combined with medicated bath bid. for 4 d.Then we detected serum bilirubin(SB)level,compared the effects of jaundice-abating between three groups. Result: Compared with control group, Observation group B was statistical significance( P ＜0.01). Compared with observation group A, the total effective rate in observation group B was raised( P ＜0.05). Conclusion: Chinese materia medica as enteroclysm combined with medicated bath is effective in the treatment of neonatal jaundice. Compared with oral administration it is convenient and deserved to spread in clinic.关键词：Xinshenger Tuihuang mixture;medicated bath;Chinese materia medica as enteroclysm;neonatal jaundice;curative effect
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发布时间：2024-01-04 - 摘要：Objective: To observe curative effect of flawless skin wounds drug for therapy of thermal skin wounds. Method: The curative effect of flawless skin wounds drug on thermal skin wounds in recent three years was summarized. Result: The total effective rate of flawless skin wounds drug treatment for thermal skin wounds is high, and the average cure duration for different degree of thermal skin wounds is short. Conclusion: The treatment for thermal skin wounds by flawless skin wounds drug with the following characteristics:fast epithelial growth, less exudation, less chance of infection, obvious analgesic effect, without toxic and side effects, short course of treatment, low cost, easy to use, inexpensive and reliable, good curative effect. So, it is worth to be used clinically.
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发布时间：2024-01-04 - 摘要：Tripterygium wilfordii Hook. f. (TWHF) belongs to Celastraceae. It is a traditional Chinese medicine, with complex chemical constituents and a variety of pharmacological effects. And it is applied for many diseases with significant effects. But the liver toxicity events caused by TWHF were often reported. Liver toxicity caused by TWHF were reviewed in this paper from four aspects including material basis, clinical features, pathological basis and mechanism, in order to provide more reference and methods for clinical safety and scientific research.关键词：Tripterygium wilfordii;liver toxicity;material basis;mechanism
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发布时间：2024-01-04 - 摘要：Metabonomics, a new science and technology, was developed in mid 1990s. It refers to a holistic analytical approach to all the low molecular weight metabolites in an organism or cells. The application of the science across the biology and pharmaceutical technology has a vast potential for future development. This paper briefly introduces the general of metabonomics, the application of metabonomics in Chinese Traditional Medicine, Chinese herbal resources, pharmacology, toxicology and clinical. Finally, the problems and future perspectives of metabolomics research are discussed and several ideas are also put forward.关键词：metabonomics;research of traditional Chinese medicine;drug evaluation;clinical application
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发布时间：2024-01-04 - 摘要：Wnt signal transduction pathway has a close relationship with cancer, as its abnormal activation plays an important role in many human cancers. Metastasis and invasion in hepatocellular carcinoma, the basic characteristics of hepatocellular carcinoma as well as important factors which influence the therapeutic effect of hepatocellular carcinoma, are closely related with Wnt/β-catenin signal transduction pathway. β-catenin is an essential regulatory factor in Wnt/β-catenin signal transduction pathway. The expression of β-catenin is positively correlated with lymph node and liver metastasis of hepatocellular carcinoma. There is also a close relationship between the metastasis of hepatocellular carcinoma and the expression of Tcf-4, which is a downstream transcription factor in Wnt/β-catenin signal transduction pathway. Therefore, the mutation and (or) aberrant expression of genes in Wnt/β-catenin signal transduction pathway set up the biomolecular basis for metastasis and invasion in hepatocellular carcinoma.关键词：hepatocellular carcinoma;Wnt/β-catenin signal transduction pathway;metastasis and Invasion
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发布时间：2024-01-04 - 摘要：Glutamate transporters play a key role in the central nervous system(CNS). Glutamate transporters comprise of excitatory amino acid transporters(EAATs) and vesicular glutamate transporters(VGLUTs).In addition to their fundamental role in mediating neurotransmitter uptake, EAATs may contribute to the modulation of a variety of cellular processes. Activation of the excitatory amino acid (EAA) carriers generates an electrogenic current attibutable to ion-coupled cotransport. Structurally distinct subtypes of human glutamate transporters, EAAT1-EAAT5 (1, 2, 11), have been identified and characterized by molecular cloning. VGLUTs transport specifically glutamates into synaptic vesicles in the presynaptic terminal for further release into the synaptic cleft. VGLUT1, VGLUT2 and VGLUT3 are three subtypes of VGLUTs. Recent studies show that many central nervous system disorders are accompanied by abnormal functions of glutamate transporters and the pathological characteristics of the disease are closely related to the particular subtypes, distribution, structures and functions of the glutamate transporters.关键词：vesicular glutamate;glutamate transporter;central nervous system disorder
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发布时间：2024-01-04 - 摘要：Objective: To summarize the experimental research progress on therapy of vascular dementia by the Chinese medicine in recent years . Method: Literature about the experimental research from the cascade of damage on vascular dementia by Chinese medicine in recent years was reviewed. Result: Chinese medicine had good therapeutic effects in the aspects of improving the learning and memory ability with vascular dementia animal models. Conclusion: Chinese medicine has its advantage on therapy of vascular dementia.关键词：vascular dementia;Chinese medicine treatment;experimental research;summarization
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发布时间：2024-01-04 - 摘要：This article introduced Gekko’s anlage and character, summed up the last ten years’s researches on its antitumor effect: active ingredient,pharmacological mechanism and clinical application,and indicated problems and research directions,in order to provide reference for its activity future research.关键词：gekko;antitumor activity;oligopeptide
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发布时间：2024-01-04 - 摘要：Objective: To introduce the research progress of superoxide dismutase (SOD) involved in liver injury. Method: Consulted the related medical journals, analyzed and compiled them. Result: SOD and its types participate in the pathological process of many types of liver injury. Conclusion: SOD plays an important role in the pathological process of liver injury. SOD and its types should be paid adequate attention to in deep studies of animal models and protective action.关键词：superoxide dismutase;liver injury;research progress
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发布时间：2024-01-04 - 摘要：With the application of infrared spectroscopy and chemometrics and other new technologies, infrared spectroscopy becomes a powerful tool in traditional Chinese medicine. Its strong ability to identify, accurate results and fast response measurement capabilities, make its application expanding gradually in the study of traditional Chinese medicine processing. The article discusses the development and application of infrared spectroscopy in the past 10 years at home and abroad in the Chinese medicine processing, and discussing its problems and prospects.关键词：near infrared;mid-infrared;processing;applications;prospect
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发布时间：2024-01-04 - 摘要：To systematically review the modern researches of the prescriptions associated to compatible theory of invigorating Qi to produce blood and explore the proper methods to research the compatible theory of invigorating Qi to produce blood.Methods and contents: 23 literatures related to invigorating Qi to produce blood, published from 1990 to 2010, were selected and summarized in terms of effective components, compatible ratio, effectiveness and it's mechanism. In addition, the problems existed in these researches were also analyzed.Conclusion: Indicating that the experimental research play emphasis on specific prescription rather than on compatible theory is not enough to explore the scientific connotation of compatible theory of invigorating Qi to produce blood.关键词：invigorating Qi to produce blood;compatible theory;experimental study
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发布时间：2024-01-04 - 摘要：Pulmonary fibrosis (PF) is a severe disease, and is one of the hot research subjects. Supplementing qi to remove stasis and phlegm is an important clinical method for treating PF in traditional Chinese medicine. Supplementing qi plays an essential role in this method. This paper reviews the research status of treating PF with astragalus, which is helpful for further exploring the anti-fibrotic mechanism of astragalus and directing its clinical application.关键词：astragalus;pulmonary fibrosis;study status
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发布时间：2024-01-04 - 摘要：Traditional Chinese herbal formula is the main drug treatment means. It reflects holistic concept and pattern identification and differentiation, and plays an important role in the theoretical system of traditional Chinese medicine. So, the modern study of Chinese herbal compound attracts wide attention. In recent years, many experts studied the pharmacology of Chinese herbal formula using molecular biological techniques and acquired a lot of fruitful results. This paper reviews the profiles of these studies and discusses them according to different types of content.关键词：Chinese herbal formula;prescription composition;molecules;gene;cells
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发布时间：2024-01-04 - 摘要：Taohe Chengqi Tang comed from the book named 〈Shanghanlun〉 which written by Zhang Zhongjing on Han Dynasty.In recent years, experimental studies on it are deeper and deeper,and its clinical applications are more broader.Research has made some progress.This paper reviewed the experimental research and clinical applications of Taohe Chengqi Tang in recent 10 years,to find out its rules and research directions.关键词：Taohe Chengqi Tang;experimental study;clinical application
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