最新刊期
卷 17 ， 期 8 ， 2011
- 摘要：Objective: Discussing the feasibility of habitat processing method of Scutellaria baicalensis compared with traditional processing method. Method: The moisture content of medicinal parts was determined before processing, and the alcohol-soluble extract content and the content of baicalin and baicalein were compared on different pieces. Result: It was easily to cut pieces with non-green phenomenon when the moisture content was in 28%-42%. The content of baicalin, baicalein and alcohol-soluble extract were similar to the traditional processing method. Conclusion: The habitat processing method not only simplifies the processing method but also avoid the hydrolysis of baicalin more effectively. It can also reduce the loss of active ingredients to ensure the quality of S.baicalensis.关键词：Scutellaria baicalensis;pieces;habitat processing
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发布时间：2024-01-04 - 摘要：Objective: To prepare pomegranate polyphenols gel and control the quality and to observe penetrability of gallic acid from the gel in vitro. Method: Carbopol-940 was used as the gel matrix, glycerin as wetting agent, potassium sorbate as a preservative and triethanolamine as neutralizer. UV-spectrometry method was used to determine total polyphenol content of the gel. The skin of rat was put between the supplying container and receiving container, and the experimentation of penetrating was carried out. Result: The preparing technology of gels was feasible. Gallic acid can be completely separated within a linear range of 0.024 5-0.245 0 g ·L-1(r=0.999 6). The average recovery was 99.56％, RSD 0.58％. The rate of penetrability in Gallic acid is 36.95％ during 24 hours. Conclusion: The preparation technique is simple and feasible for the quality control. It shows excellent transdermal effects.关键词：pomegranate polyphenols;gel;carbopol-940;skin of the rat;transdermal effects
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发布时间：2024-01-04 - 摘要：Objective: To study the preparation technology of the inclusion complex of borneol with β-CD and HP-β-CD, and to compare their stability. Method: The borneol was included with β-CD and HP-β-CD. GC method was used to investigate their stability. Result: Borneol that were included by β-CD and HP-β-CD could prevent the volatilization of borneol and promote the stability of the drug. Conclusion: The stability of borneol included with HP-β-CD and β-CD was improved.关键词：borneol;β-CD;HP-β-CD;inclusion
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发布时间：2024-01-04 - 摘要：Objective: To investigate the purification processes of salvianolic acid B and paeoniflorin from Fufang Jingzhiguanxin with macroporous adsorption resin. Method: To measure the content of salvianolic acid B and paeoniflorin with HPLC. To evaluate the optimal macroporous adsorption resin. Result: The optimal macroporous adsorption resin was D-101.The optimal purification conditions: The sample concentration was 0.2 g ·mL-1. The diameter height ratio was 1 ∶7.The sample volume was 8 BV. The eluant was 40% ethanol. The elution flow rate was 3 BV ·h-1. The ethanol consumption was 4 BV. Conclusion: D-101 has a good purification effect under the above conditions.关键词：macroporous adsorption resin;salvianolic acid B;paeoniflorin;purification
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发布时间：2024-01-04 - 摘要：Objective: To study the percutaneous absorption of strychnine in Shangke spray in vitro. Method: The transdermal permeation tests of strychnine in Shangke sprays were performed through excised rats skin in Franz diffusion cells. Strychnine concentration was determined by HPLC. Result: Strychnine can penetrate through rat skin. The permeation rate and the lag time were 0.015 3 mg ·cm-2 ·h-1 and 0.235 h respectively. Conclusion: The research provides experimental data for the design of Shangke sprays.关键词：Shangke sprays;strychnine;percutaneous absorption
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发布时间：2024-01-04 - 摘要：Objective: to study practicability on separating passive absorption active constituents of aqueous extract of traditional Chinese medicine(TCM) by liposome liquid membrane extraction technology. Method: The aqueous extract of TCM such as Fructus Evodiae and Herba Ephedrae were extracted by liposome preparaed respectively by ammonium sulfate transmembrane gradients method, citric acid transmembrane gradients method and sodium glutamine transmembrane gradients method by liquid membrane extraction technology. The active constituents extracted by liposome were determined by RP-HPLC. Result: Active constituents such as ephedrine and evodiamine which were passively absorbed much could be extracted easily by liposome, and the extracted rates were all more than 50%. Active constituents such as glycyrrhizic acid, chlorogenic acid, puerarin and paeoniflorin which were passively absorbed little could be extracted hard by liposome, and the extracted rates were all no more than 10%. The extracted rates were affected by the factors such as varieties of liposome, ratio of liposome and aqueous extract of TCM, time of extracting and temperature of extracting. Conclusion: Separating active constituents of aqueous extract of TCM by liposome liquid membrane extraction technology is feasible.关键词：liposome;liquid membrane extraction
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发布时间：2024-01-04 - 摘要：Objective: To screen the suitable resin for purification of saponin from sapindaceae and to establish the optimum technological parameters of purification. Method: According to the retention rate of the hederagenin, four types of macroporous resin were used to purify the saponin from sapindaceae. Result: The best conditions was: D-101 of macroporous resin; the concentration of ethanol eluate was 50%; the usage of ethanol was 5 BV. Conclusion: D-101 of macroporous resin showed good capability of isolation and purification of saponin from sapindaceae.关键词：sapindaceae;hederagenin;macroporous resin;isolation and purification;HPLC
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发布时间：2024-01-04 - 摘要：Objective: Using multi-index comprehensive evaluation method to provide the production technology parameters of Wuji Wan. Method: The orthogonal test was employed with the index of the rate of extraction and the content of total alkaloid, paeoniflorin, evodiamine and rutaecarpine, optimizing the extraction process of Wuji Wan by multi-index comprehensive evaluation method. Result: Extraction times had much notable effect on experimental results and times, and the amount of ethanol had notable effect on the results. Ethanol concentration and extraction time had no notable effects. Considering the effects of five factors synthetically, the optimum process was A3B3C3D1, that was 80% ethanol,10 times the amount of ethanol, extraction 3 times and 1hour every time. Conclusion: The experimental method was steady and trusty, which will have some instruction meaning and reference value to the production of Wuji Wan.关键词：Wuji Wan;the orthogonal test;comprehensive evaluation method
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect on total flavonoids’content of compound Pyrola with different extraction process and optimize the best extraction process. Method: Flavonoid compounds were extracted from compound Pyrola with different solvent, water and alcohol respectively. Orthogonal test was used to determine the best extraction process of compound Pyrola flavonoids with the content of total flavones as the marker. Result: The more effective way to extract total flavones was extraction with 20 times amount of 60% alcohol under reflux for 1 hour. Conclusion: This method obtains more effective components. It is stable and reliable.关键词：total flavones;orthogonal test;compound Pyrola
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发布时间：2024-01-04 - 摘要：Objective: To optimize a technique for extracting and purifying total tanshinones from Salvia miltiorrhiza. Method: Total tanshinones were selected as marker components and determined to optimize extraction and purification process of Salvia miltiorrhiza by orthogonal test. Result: The optimum extraction process was as follows: Salvia miltiorrhiza was added with 8 times alcohol of 90% and extracted 1 hour for 2 times. The optimum extraction purification was as follows: D101 macro porous resin was adopted in the experiment.The concentration of sample was 40%, the adsorptive speed of flow was 3 BV ·h-1. The adsorbing column was eluted with 5 BV water to remove the sugar. Finally the adsorbing column was eluted with 6 BV 90% ethanol and the flow rate was 5 BV/h. Conclusion: The content of the total tanshinones obtained was high by the optimum purification conditions. The technique is simple and suitable for the modern production.关键词：total tanshinone;orthogonal design;macro porous resin
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发布时间：2024-01-04 - 摘要：Objective: To optimize selection of collagen sponge freeze-drying process, such as collagen protein molecules of freeze-drying reference. Method: Orthogonal test was used to choose the best collagen sponge freeze-drying process with water imbibition and re-hydration values as index. Result: The rate of pre-freezing was 0.35 ℃ ·min-1, the duration of pre-freezing was 1.5 h, the lowest temperature of pre-freezing was -35 ℃ and the temperature of the first sublimation was -10 ℃. Conclusion: The pigskin collagen sponge produced by the freeze-drying process which has been chosen is osteoporosis and porous, the water imbibition and re-hydration of the pigskin collagen sponge are well.关键词：collagen sponge;freeze-drying;technology research
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发布时间：2024-01-04 - 摘要：Objective: To study and optimize the extraction and preparation technics of icariin and total flavonoids by orthogonal design. Method: The best conditions of extracting icariin and total flavonoids from Herba Epimed by flash-type extraction were screened by using method of orthogonal design and selecting the amount of icariin and total flavonoids as index. Result: The effect influencing factors of flash-type extraction were as follows: extraction times＞duration of extraction>ratio of solid to liquid>concentration of ethanol. The optimum extraction condition obtained was: 10 minutes and 3 times, ratio of solid to liquid 1 ∶25, 50% ethanol. Conclusion: Flash-type extraction was found to be an efficient and rapid method for extracting total flavonoids and icariin from Herba Epimedii.关键词：Herba Epimedii;flash-type extraction;total flavonoids;icariin;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To study formulation and preparation of Gutong film coating tablets. Method: The single-factor method was taken to optimize the pharmaceutical formulations, the content was selected by granule properties, granulating difficulty, angle of repose, degree of surface smoothness, hardness and disintegration. Result: The optimum process was as follows:the weight proportion of dry extraction to starch, lactose and silica powder was 1 ∶0.5 ∶0.4 ∶0.1; the wetting agent was 85% alcohol; through wet-granulating, tabletting and film coating, the Gutong tablets were prepared successfully. Conclusion: The formulation of Gutong film coating tablets is reasonable and the process technical is simple and feasible, which conforms to the general requirements of production.关键词：Gutong tablets;film coating;process technical
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发布时间：2024-01-04 - 摘要：Objective: To optimally select the extraction technology of total flavonoids of Lycium barbarum leaves by ultrasonography and to determine the content of total flavonoids of L.barbarum leaves of different picked time. Method: The effects of four single-factors (including ethanol concentration, ethanol consumption, ultrasonic extraction time and temperature)on the extraction of total flavonoids of L. barbarum leaves have been studied.And the results of the orthogonal experiment were analyzed by variance analysis. Use the best extraction technology to determine the contents of total flavonoids in leaves of L.barbarum at different picked times. Result: The best single factor was determined and the best extraction of total flavonoids of L. barbarum leaves by ultrasonography with orthogonal experiment was optimized. The best extraction technology were as follows: using 65% ethanol as extractor with the ratio of material to liquid at 1 ∶60 and extraction for 35 min in 70 ℃. Conclusion: By comparing the contents of total flavonoids in leaves of L. barbarum at different picked time, the contents of total flavonoids in leaves were decreased with the leaves growing. It is conclude that the best picked time of L. barbarum is the middle of May.关键词：leaves of Lycium barbarum;total flavonoids;ultrasonic extraction;picked time
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发布时间：2024-01-04 - 摘要：Objective: To study the individual quality evaluation standard of raw rhubarb and stewed Rhubarb. Method: Qualitative and quantitative identification of two pieces from rhubarb were performed by using the thin-layer chromatography, HPLC fingerprint chromatogram. Result: The change of quantity and content in pieces could be reflected directly and clearly in HPLC fingerprint chromatogram and the TLC in which two kinds of mobile phase were used. There were notable differences in the content of 5 kinds of free anthraquinone, anthraquinone glycoside, stilbene glycoside, catechin and gallic acid in stewed Rhubarb and raw rhubarb. Conclusion: Suggestion on quality standard of raw rhubarb and stewed Rhubarb should be taken differently by the indicator including the content of free anthraquinone, anthraquinone glycoside, catechin, gallic acid and stilbene glycoside. The emodin-8-O-β-D-glucoside and the catechin are the characteristics index of raw rhubarb.关键词：raw rhubarb;stewed rhubarb;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To study and establish the quality control methods for Baiqilong capsules. Method: The method of TLC was used to identify Hedyotis diffusa, Gekko swinhoana, Radix Astragali and Poria cocos. The content of astragaloside IV was determined by HPLC. Result: Four herbal drugs were well identified by TLC, the spots were clear, round and without interference. Quantitative analysis of HPLC showed that the linear range of astragaloside IV was 1.354~10.832 μg. The average recovery of astragaloside IV was 99.59%, and the RSD was 1.25% (n=6). Conclusion: The method set up is simple and feasible, and could effectively control the quality of Baiqilong capsules.关键词：Baiqilong capsules;quality control;Gekko swinhoana;astragaloside IV
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of standard extract from Coptidis Rhizoma and establish quality control method of it. Method: L9(34) orthogonal experiment was used to optimize the extraction process of CoptidisRhizoma. The contents of berberine hydrochloride, palmatine hydrochloride and coptisine hydrochloride were detected simultaneously by HPLC. Result: The optimal condition for extraction were as follows: most coarse powder of Coptidis Rhizoma was reflux extracted with 10 times 60% ethanol for 1 h, for three times. The extraction yield was 25.47%. The contents of berberine hydrochloride, palmatine hydrochloride and coptisine hydrochloride were 23.02%, 5.63% and 5.72%, respectively. The calibration curve of berberine hydrochloride was linear in the range from 8.5 to 271.2 mg ·L-1 (r>0.999 9) and the average recovery was 100.14% (RSD 1.92%). The calibration curve of palmatine hydrochloride was linear in the range from 4.2 to 133.2 mg ·L-1 (r>0.999 9) and the average recovery was 98.52% (RSD 1.65%). The calibration curve of coptisine hydrochloride was linear in the range from 3.1 to 97.2 mg ·L-1 (r>0.999 9) and the average recovery was 99.80% (RSD 1.45%). Conclusion: Reflux extraction with ethanol was effective method for preparation of standard extract form Coptidis Rhizoma. HPLC methods established can be used for quality control of the standard extract.关键词：Standard extract from Coptidis Rhizoma;berberine hydrochloride;palmatine hydrochloride;coptisine hydrochloride;orthogonal experiment;HPLC;preparation;quality control
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standards of the method for determination of small bionic zymolysis peptides of leeches. Method: Biure method, Lorry method in China pharmacopoeia 2010 (vol.Ⅱ and vol.Ⅲ), the ninhydrin colorimetry method were used to determine the content of total peptide with amino acid analyzer determination result for reference. Result: The content of total peptide determined by Lorry method in China pharmacopoeia 2010 (vol.Ⅲ) was not only accurate but also repeatable and stable. Conclusion: Determination with Lorry method in China pharmacopoeia 2010 (vol.Ⅲ) is confirmed as a valid content determination method of small bionic zymolysis peptides of leeches.关键词：leeches;content determination;biuret method;Lowry method;ninhydrin colorimetry method
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发布时间：2024-01-04 - 摘要：Objective: The volatile constituents in aerial part and rhizome of Osmunda japonica.were analyzed by head-space solid micro-extraction,coupled with GC-MS. Thirty-one compounds and 27 compounds were identified from the aerial part and the rhizome of O.japonica separately.Eight compounds were mutual, account for 13.99% of total compounds in the aerial part and for 40.04% in the rhizome. It indicated that the volatile constituents in aerial part of O.japonica are quite different from in rhizome.关键词：HS-SPME-GC/MS;Osmunda japonica;volatile constituents
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发布时间：2024-01-04 - 摘要：Objective: To study the soluble calcium ion content of different compatibility of Maxing Shigan tang and to discuss the compatibility regularity of Maxing Shigan tang. Method: The ICP-AES was used to study the soluble calcium ion content of each single herb about their different compatibility and different soaking and boiling time in Maxing Shigan tang. Result: All of herbs except the Almond in Maxing Shigan tang contained calcium. Gypsum had the highest content of calcium, followed by Ephedra, Licoroce had the lowest. The calcium ion content was decreased when Gypsum compatible with other herbs. Gypsum decoction had the highest content of calcium, followed by Gypsum Ephedra, Gypsum Licoroce and Gypsum Almond groups; Gypsum Ephedra Licoroce group had the lowest content of calcium. The calcium ion content of Maxing Shigan tang group was higher than that of Gypsum Ephedra Licoroce group. The calcium ion content of gypsum and Maxing Shigan tang group was increased with extend time of soak, but was increased first and then decreased by extending the boiling time. Especially, in the group of Gypsum and Maxing Shigan tang theoretical value, the calcium ion content declined rapidly. Conclusion: The calcium ion content can be decreased when Gypsum compatible with other herbs and increased by extending the soaking time. The calcium ion content was increased first and then decreased when extending the boiling time. The Gypsum compatible with Ephedra, Licoroce and Almond will not increase the calcium ion content, but can keep it in a stable range.关键词：Maxing Shigan tang;ICP-AES;content of calcium ion;Gypsum;compatibility
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of anti-osteoporosis active extract of the processed Cibotium baronetz. Method: The components of C.baronetz were isolated on silica gel column chromatography and their structures were elucidated by chemical properties and spectroscopic evidence. Result: Eight constituents were isolated and identified as onitin (1), pyromeconic acid (2), maltol (3), kojic acid (4), 5-hydroxymaltol (5), protocatechuic aldehyde (6), caffeic acid (7), protocatechuic acid (8). Conclusion: Four α-hydroxy- 4H-pyran-4-one compounds were isolated from processed C.baronetz for the first time.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the parameters of serum pharmacochemistry of Dihuang Yinzi. Method: Using UPLC-ESI-MS method, the chromatograms of rabbit serum samples after oral administration of Dihuang Yinzi and crude drugs were analyzed and compared with control rabbit serum. Result: Nine constituents absorbed into the blood after administration of Dihuang Yinzi were detected, including seven metabolites and two original form compounds. Conclusion: The constituents absorbed into blood and their metabolites of Dihuang Yinzi were identified preliminary. This study is conducive to the exploration of bioactive constituents of Dihuang Yinzi.关键词：Dihuang Yinzi;serum pharmacochemistry;constituents absorbed into blood
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发布时间：2024-01-04 - 摘要：Objective: To establish a sensitive HPLC fingerprint method for controlling the quality of compound Jiangtang dripping pills(CJDP). Method: HPLC analysis was performed on a Hypersil BDS C18 column (4.6 mm ×250 mm,5 μm),with a mixture of acetonitrile and water as mobile phase in gradient mode. The wavelength was set at 238 nm, and column temperature was 30 ℃. Result: Five peaks were identified in the HPLC fingerprint of CJDP and their similarities were more than 0.99.Good precision, stability and repetition were showed. Conclusion: This method provides an evidence to quality evaluation of CJDP.关键词：compund Jiangtang dripping pill;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To analyze and compare the chemical constituents of the volatile oil from fresh and dried Rhizoma Musae. Method: Essential oils obtained by hydrodistillation from fresh and dried Rhizoma Musae which came from the same plant, was analyzed by GC-MS. Result: Thirty-six compounds were characterized, representing 76.28% of the fresh Rhizoma Musae volatile oil,whereas in the dried Rhizoma Musae oil,only 32 compounds were characterized, representing 81.78 %. Conclusion: Among them, the majority were pentadecanal, squalene and heptenal. Their relative content was 19.60%,11.46%,8.69% in fresh Rhizoma Musae oils and 17.37 %,8.87%,5.07 % from dried Rhizoma Musae, respectively. The relative contents of other chemical constituents were different.关键词：Rhizoma Musae;essential oil;gas chromatography-mass spectrometry (GC-MS)
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发布时间：2024-01-04 - 摘要：Objective: The fatty acid components from the rhizome of Tupistra chinensis produced in Hanzhong and Liuba of the Qinling-Daba mountains in Shaanxi province were extracted by soxhlet extraction. Method: The experiment were identified and determined by gas chromatography-mass spectrometry(GC-MS) after methyl esterification treatment. Result: The relative percentage of each component was confirmed by peak-area normalization method. Total 29 kinds of fatty acid were identified from T. chinensis produced in Liuba on Southern Slope of Qinling mountains. The main components of fatty acids were hexadecanoic acid(42.815%),9,12-octadecadienoic acid(11.386%),9-octadecenoic acid(6.378%),octadecanoic acid,8,10-dimethoxyl(6.355%),octadecanoic acid(4.18%),octadecanoic acid,9,10,12-trimethoxyl(3.724%),etc. Total 31 kinds of fatty acid were identified from T.chinensis in Nanzheng on Northern slope of Bashan mountain. The main components of fatty acids were hexadecanoic acid(32.404%),9,12-octadecadienoic acid(10.144%),hexacosanoic acid(5.466%),octadecanoic acid,8,10-dimethoxy(5.418%),9-octadecenoic acid(4.675%),octadecanoic acid(4.664%), octadecanoic acid,9,10,12-trimethoxyl(4.638%),octadecanoic acid, 9,11,13-trimethoxyl(4.422%),docosanoic acid(4.234%),etc. Conclusion: It was shown that there were some impotent fatty acids in T.chinensis which have potential development and application value in food nutrition and health care.关键词：Tupistra chinensis;fatty acids;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the active constituents of storax. Method: The constituents were isolated with silica gel and Sephadex LH-20 gel column chromatography and purified by reversed phase ODS column chromatography. Their structures were elucidated by spectroscopy. Result: Ten compounds were obtained and identified as pimaric acid (1), isopimaric acid (2), dehydroabietic acid (3), abietatrien-3β-ol (4), oleanoic aicd (5), oleanonic acid (6),p-hydroxycinnamic acid (7), vanillin (8), vanillic acid (9), 5-hydromethyl furaldehyde (10). Conclusion: Compound 1-9 are obtained from this plant for the first time.关键词：storax;chemical constituents;NMR
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发布时间：2024-01-04 - 摘要：Objective: The chemical composition of essential oils isolated from the leaves, stem and flowers of Dichrocephala integrifolia were investigated. Method : The essential oils of D. integrifolia were extracted by solid-phase microextraction(SPME), then separated and analysed by gaschromatography-mass-spectrometry (GC-MS). Result: Thirty four components were identified in the oils of flowers with germacrene-D(35.71%), β-pinene (15.35%), β-cis-ocimene(10.95%), 2, 4-diisopropenyl-1-methyl-1-vinylcyclohexan (6.64%), γ-elemene(3.90%). Phthalic-acid, diisobutyl ester(28.25%), α-bisabolol(9.18%), β-pinene(8.44%) and phthalic acid, butyl octyl ester (6.90%) were found to be predominant constituents in the oils of stem and leaves. Conclusion: β-Selinene was the major constituent of both the oils studied (15.35% and 8.44 %, respectively).关键词：Dichrocephala integrifolia;essential oils;chemical constituents;SPME;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: The samples of Mongolian medicinal plants of Scabiosa were analyzed by microwave digestion-inductively coupled plasma-atomic emission spectrometry (ICP-AES) for content of many trace elements including Al, As, B, Ba, Cd, Co, Cu, Fe, Hg, In, Mn, Pb, Sr and Zn. Method: A mixture of nitric acid and hydrogen peroxide was used for microwave digestion of samples. Result: The method exhibited a spike recovery range of 96.6% to 110.6%, with RSDs of less than 4.29%. Under optimized experimental conditions, a simultaneous determination of various trace elements was achieved without any interference among them. Conclusion: The findings will provide useful evidences for the better exploration and utilization of Mongolian medicinal plants of Scabiosa.关键词：ICP-AES;trace elements;Mongolian medicine;Scabiosa
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发布时间：2024-01-04 - 摘要：Objective: To determine the contents of Zn,Cu,Fe,Co,Mn and Cr in Chinese medicine of Liuwei Dihuangwan by atomic absorption spectrometry, studying on the relationship between the trance elements and the composition of Liuwei Dihuangwan. Method: The factor analysis was used to investigate the relationship between the trance elements and the principle of Liuwei Dihuangwan composition and indication by the guiding of Chinese medicine. Result: Factor analysis revealed the two structure of Liuwei Dihuangwan, pointed out that efficacy of Fructus Rehmanniae Praeparata, Fructus corni, Rhizoma dioscoreae and Fe,Cr,Co,Mn function correlation; that efficacy of Poria, Cortex moutan, Rhizoma alismatis and Zn,Cu function correlation.The composition of Chinese medicine in prescription and arrange in dimensional affect were same as the complaining of Chinese medicine. Conclustion: The principle and indication of Chinese medicine are related to trance elements.关键词：trance elements;Liuwei Dihuangwan;principle of composition;indication
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC fingerprint for the identification of Smilax china. Method: The analysis was carried out on a Diamonsil C18(4.6 mm×250 mm,5 μｍ)with a mobile phase consisting of acetonitrile-0.1 % phosphoric acid, gradient eluent, the flow rate of 0.6 mL ·min-1. The wave length was 330 nm, data were analyzed by fingerprint similarity evalution software to compare the similarity of samples. Result: The mutual mode to HPLC fingerprints was estabilished,and the 18 mutual peaks were indicated.The similarities among the of S. china and S. glabra collected from different sources were obtained. there were apparent difference in fingerprint. Conclusion: HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Musa basjoo.关键词：Smilax china;HPLC Fingerprint;identification
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of ethyl ether spirit of seed coat of Iris. Method: To analyze and identify the chemical constituents by GC-MS. Result: We separated the extraction by GC-MS method, and affirmed 26 peaks by NIST workstation and reference documentation. The contents of irisquinone, (E)irisquinone isomer and pallasone B were high and main types of the constituents were saturated fatty acid ester, unsaturated fatty acid ester, fragrance ester, organic acid, and alkaloid etc. Conclusion: Analyzing and identify composition by GC-MS is fast, stable, and has fine reproduction, it can be used for composition analysis of seed coat of iris.关键词：Iris pallasiui;chemical constituent;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To investigate the fingerprint for methanol extraction of Muxiang Shunqi pills by HPLC-UV. Method: The samples were separated with an Agilent Zorbax Eclipse XDB C18 reversed-phase column(4.6 mm×150 mm, 5 μm) by linear gradient elution using water-acetonitrile as mobile phase; the flow rate was 1 mL ·min-1 and the detection wavelength was set at 280 nm, 225 nm(rosa banksiae); the column temperature was set at 35 ℃. Muxiang Shunqi pills from different pharmaceutical company were compared and the individual crude drugs were analyzed. Result: Honokiol was linear in the range of 104-520 mg ·L-1, Y=70 580X+1.187 5. r=0.999 7; RSD of all relative retention time and relative peak area achieved good results, the precision test and stability test satisfied the requirements and repeatability test was less than 1% and 3% respectively. Conclusion: The quality of Muxiang Shunqi pills is obviously different; one of the main reasons of such state is the quality of crude drugs.关键词：Muxiang Shunqi Pills;Pericarpium citri reticulatae;Cyperi Rhizoma;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective: By determining the active compositions and other compositions in rhizome of Atractylodes lancea from geoherbs and herbs in main producing areas in order to synthetically evaluate the quality of them. Method: β-eudesmol, atractylon, atractylodinol and atractylenolideⅡ in rhizome of A.lancea in different regions were determined by HPLC. Result: There were great differences in content and quality among different regions of A. lancea. The highest content of β-eudesmol and atractylodinol was found in wild products grown in Yingshan city Hubei Province, while the highest content of atractylon and atractylenolideⅡ was found in maoshan city Jiangsu province. Conclusion: The quality in the main origins was better than that in authentic origin from the content determination of β-eudesmol, atractylon, atractylodinol and atractylenolideⅡ in different places, which provide the basis for industrial development of lancea.关键词：Atractylodes lancea;β-eudesmol;atractylon;atractylodinol;atractylenolideⅡ;HPLC;determination
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC-ELSD method for the determination of diosgenin in Dioscorea nipponica and comparison of content by ELSD and DAD. Method: An Agilent Eclipse XDB-C18(4.6 mm×150 mm, 5 μm) was used with the mobile phase of methanol-water(95 ∶5) at 40 ℃. Evaporated light scattering detector was used. The drift tube temperature was at 70 ℃ and the pressure of nebulizing gas was 367.5 Pa, gain was 7, filter was 1.The injected volume was 10 μL. Result: The linear calibration curve was obtained in the ranges of 2.60-20.8 μg for diosgenin(r=0.999 94). The average recovery was 97.3% with RSD of 1.94%. Conclusion: This method is simple, sensitive and accurate. It can be used for the quality control of D. nipponica Makino. This method achieves more flat baseline, higher sensitivity, better resolution and higher content than DAD.关键词：Dioscorea nipponica;diosgenin;ELSD;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of berberine hydrochloride, phellodendrine and timosaponin BⅡ in common anemarrhena rhizome and amur corktree bark extraction. Method: HPLC-UV-ELSD was used in quantitative analysis with condition as follows: Dikma C18(4.6 mm×250 mm, 5 μm) was used in analysis; mobile phase was acetonitrile-0.2% acetic acid, gradient elution; flow rate was 1.0 mL ·min-1; column temperature was at 30 ℃. Both UV and ELSD were used as detector. UV wavelength of 280 nm; evaporative light scattering detector drift tube temperature was at 46 ℃; nitrogen pressure: 1.5 Bar. Result: The linear ranges of berberine hydrochloride, phellodendrine and timosaponin BⅡ were 1.008-3.528 μg(r=0.999 5), 0.322-1.127 μg(r=0.999 6), 0.702-2.457 μg(r=0.999 8), the average recoveries were 100.11%, 98.97%, 99.56% with the RSD 1.08%, 0.90%, 1.37%. Conclusion: This quantitative determination method is simple, well reproducible, accurate and reliable.关键词：common anemarrhena rhizome and amur corktree bark extraction;berberine hydrochloride;timosaponin BⅡ;HPLC-UV-ELSD
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the essential oils from Magnolia coco. Method: The essential oils were extracted by steam distillation(SD) and supercritical carbon dioxide extraction(SFE), and then the constituents were separated and identified by GC-MS. Result: Fifty six compounds were isolated and identified by SD that composed about 87.46% of the total essential oils; and 25 compounds were isolated and identified by SFE that composed about 95.86% of the total essential oils. Conclusion: The principal constituents of essential oils in M.coco from Guangxi are α-pinene, nerolidol, caryophyllene, germacrene D, bicyclogermacrene, which are very different from the other origin.关键词：Magnolia coco;essential oils;steam distillation;supercritical carbon dioxide extraction;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To develop a quantitative reversed phase high performance liquid chromatography method for determination of R,S-goitrin in Banlangen preparations. Method: The samples were separated at 30 ℃ on a Shiseido C18 MG( 4.6 mm×250 mm,5 μm) column eluted with methanol-0.02% phosphate(7 ∶93) as the mobile phase. Flow rate was set at 1.0 mL ·min-1 and the detection wavelength was set at 245 nm. Result: The calibration curve of R,S-goitrin was linear within the range from 0.004 9 to 0.312 0 g ·L-1(r=1.000 0) and the average recovery was 99.0 %(RSD 2.04 %). Conclusion: The developed method was proved to be simple, reliable and useful by analysis of 26 batches of the samples purchased from different areas.关键词：R,S-goitrin;banlangen preparations;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop a RP-HPLC method for simultaneous determination of two flavonoids, quercetin-3-O- -5-O-β-D-glucopyranoside(compound I) and quercetin-3-O- -5-O-β-D-glucopy-ranoside(compound II) in the seeds of Impatiens balsamina. Method: An AlltimaTM-C18 column(4.6 mm×250 mm, 5 μm) was used at 35 ℃ with the mobile phases of methanol-water(containing 0.1% acetic acid) in gradient manner(0-10 min, 25%-28%A, 11-12 min, 28%-34%A, 13-20 min, 34%-35%A, 21-28 min, 35%-41%A, 29-35 min, 41-25%A). The flow rate was 1.0 mL ·min-1, and the detection wavelength was 345 nm. Result: The linearity was in the range of 0.210-4.200 μg and 0.206-4.120 μg, the correlation coefficients(n=6) were 0.999 0 and 0.999 8; the average recoveries(n=6) were 98.26% and 101.73% for two flavonoids respectively. Conclusion: Determination of two flavonoids in the seeds of Impatiens balsamina was developed for the first time, and provided a reference for the quality standard of it.关键词：Impatiens balsamina;flavonoids;RP-HPLC;quantitative determination
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发布时间：2024-01-04 - 摘要：Objective: The volatile compounds from Ainsliaea fragrans were studied. Method: Solid-phase micro-extraction(SPME) was used to extract the volatiles. The chemical constituents were analyzed by gas chromatography-mass spectrometry(GC-MS) coupled with Kovats indece. A quantitative analysis in percent was performed by peak area normalization measurements. Result: Fourteen compounds were identified, 59.92% of total volatiles. Conclusion: The main compounds were 3-ethyl-8-methyl-S-triazolo pyrazine(43.37%), caryophyllene(8.89%), Z,Z,Z-1,5,9,9-tetramethyl-1,4,7-cycloundecatriene(2.47%).关键词：Ainsliaea fragrans;volatile compounds;SPME;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: HPLC method prescribed in the main component of puerarin content. Method: HPLC to Kromasil C18(4.6 mm×250 mm, 5 μm) column for the analysis of the mobile phase was methanol-water(27 ∶73), flow rate 1 mL ·min-1, detection wavelength 250 nm, Result: puerarin regression equation Y=93 128X+92 301(r=0.999 5, n=6), the scope of puerarin 0.06-0.18 μg a good linear relationship. The average recovery was 100.53%, RSD 1.41% precision. Conclusion: The method is simple, rapid, accurate, sensitive and suitable for quality control of Kanger skin.关键词：Kangerfu Capsul;puerarin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of vitamin C in Xierkang syrup. Method: Capillary electrophoresis, 3.5 g ·L-1 boric acid-3 g ·L-1 borax (pH 8.7) was used as running buffers and benzoic acid as internal standard. The separation voltage was set at 12 kV and the detection wavelength at 243. Result: The linear range of vitamin C was 0.112-1.12 g ·L-1. The average recovery was 99.6%. Conclusion: The method is simple, rapid, and sensitive and it could be used for the quantitative analysis of vitamin C in Xierkang syrup.关键词：Xierkang syrup;Rosa roxburghii;vitamin C;capillary electrophoresis
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发布时间：2024-01-04 - 摘要：Objective: To identify microscopically the root, stem and leaf of Gendarussa vulgaris. Method: The paraffin section and temporary water tablets were used to identify and analyse the microscopic structure. Result: In the older root of G.vulgaris, there is a thin cork layer outside, then the velamen.The cambium has become ring with xylem development and pith in center. The stem has multiple epidermis, clear endodermis, stone cells into ring, and some small calcium oxalate crystals or column crystal in its pith parenchyma cells. In addition, the material also has cystolithes in the epidermal cells of stem and leaf, diacytic type of stoma and glandular scales. The palisade tissue is short, and through the veins. Conclusion: The results provide new information for identification, formulation the quality standards of G. vulgaris Nees.关键词：Gendarussa vulgaris;microscopic identification;cross section
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of shading on chemical constituents of Pinellia peda-tisecta. Method: Four nitrogenous compounds contents of them were measured under different treatments(0%, 40%, 60%, and 80% of shade rate) by a HPLC. Result: The results showed that four nitrogenous compounds contents of P.peda-tisecta increase then decrease with the increase of shade rate. Compared with full light, the contents of trigonelline and thymine increse under A,B,C treatment, the contents of amidopurine and hypoxanthine increase under A,B treatment and decrese under C treatments. Conculusion: Under moderate shade, which will help increasing four nitrogenous compounds contents, it is suitable for cultivation under moderate shade, it is suitable for cultivation forest(fruit) intercropping.关键词：Pinellia peda-tisecta;shading;nitrogenous compounds
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of two treating principles and formulas, named 'invigorating spleen to remove phlem and promoting blood circulation to remove meridian obstruction’(Jianpi) and 'invigorate the kidney and promoting blood circulation to remove meridian obstruction’(Bushen), on the expression of osteoprotegerin(OPG) and receptor activator of nuclear factor-κB ligand(RANKL) mRNA in steroid-induced osteonecrosis of the femoral head(SONFH), as well as to explore and compare their mechanism of prevention and treatment for SONFH. Method: Sixty-four chickens were randomly divided into control group, model group, Jianpi group and Bushen group. Except the control group, the animal model of SONFH was established with injection of methylprednisolone in chest muscle of chickens. At 8th and 16th weeks, both sides of femoral heads were taken off to observe the expression of OPG and RANKL mRNA with in situ hybridization staining. Result: Compared with the control group, the expression of OPG mRNA was decreased and RANKL mRNA increased significantly in model group(P<0.01). Compared with model group, both in Jianpi and Bushen group, the expression of OPG mRNA was increased and RANKL mRNA decreased significantly, meanwhile the ratio of OPG to RANKL was increased(P<0.01 or P<0.001). In Bushen group, the change did not obvious at the 8th weeks, however, the changes were significant compared with that in Jianpi group at the 16th weeks. Conclusion: Both Jianpi and Bushen formula can regulate the expression of OPG and RANKL mRNA to inhibit bone destruction, thereby exert preventive and therapeutic effect for SONFH. Jianpi take action earlier than Bushen formula.关键词：osteonecrosis of the femoral head;osteoprotegerin;receptor activator of nuclear factor-κB ligand
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发布时间：2024-01-04 - 摘要：Objective: To find out the best prescription composition of reinforcing Qi and enriching blood by factorial design in hemorrhagic blood deficient mouse. Method: The factor A with 12 levels was composed with 12 of reinforcing Qi medicine, such as Ginseng Radix et Rhizoma, Panacis Quinquefolii Radix, Codonopsis Radix, Pseudostellariae Radix, Astragali Radix, Atractylodis Macrocephalae Rhizoma, Dioscoreas Rhizoma, Glycyrrhizae et Rhizoma Radix, Jujubae Fructus, Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis,Ganoderma and factor B with 6 levels was composed with 6 of enriching blood medicine, such as Angelicae Sinensis Radix, Rehmanniae Radix, Pblygoni Multiflori Radix, Paeoniae Alba Radix, Corii Asini Colla, Longan. The medicines composed 72 prescriptions of reinforcing Qi and enriching blood according to 12 × 6 factorial design. The hemorrhagic blood deficient model of mouse was established with 0.5mL orbital bleeding. Peripheral blood cells were counted after 1, 4, 7, 10 d of modeling. The repeated measures of factorial design were used to analyze the data. Result: There was no interaction between reinforcing Qi medicine and enriching blood medicine.The difference in effect of the prescriptions of reinforcing Qi and enriching blood on RBC was significant at different time points(P<0.01). The difference in effect of different levels of reinforcing Qi on RBC, HGB and HCT was significant(P<0.01), and the Astragali Radix was the best. The effect of enriching blood medicines on RBC, HGB and HCT was all significant,and Angelicae Sinensis Radix was the best. The composition of Astragali Radix and Angelicae Sinensis Radix showed significant effect of enriching blood. Conclusion: The influence of reinforcing Qi medicine on the prescriptions is more strong than that of the medicines of enriching blood. Composition of Astragali Radix with Angelicae Sinensis Radix appears to be the best compatibility for the efficacy.关键词：factorial design;medicine of reinforcing Qi;medicine of enriching blood;hemorrhagic blood deficient;best compatibility for efficacy
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发布时间：2024-01-04 - 摘要：Objective: To observe the hypoglycemic effect of the Astragalus Hypoglycemic Granule on type 2 diabetic mice caused by streptozotocin(STZ). Method: Eighty SPF male C57BL/6 mice of 4-5 weeks old were given the diet of maintenance fodder for one week. After that 10 mice were selected randomly to form the normal group. Given high fat forage diet and intraperitoneal injection of STZ of 60 mg ·kg-1,type 2 diabetic model was established for the other 60 mice. Then, they were randomly divided into low dose of Astragalus Hypoglycemic Granule group(1.0 g ·kg-1), middle dose of Astragalus Hypoglycemic Granule group(2.0 g ·kg-1), high dose of Astragalus Hypoglycemic Granule group(3.0 g ·kg-1), metformin hydrochloride group(0.3 g ·kg-1), Ginseng-Astragalus Hypoglycemic Granule group(0.2 g ·kg-1), and model control group(n=10 in each group). The mice in normal and model control groups were given distilled water once a day and the ones in other groups were administrated medicine treatment by ig once a day for 14 days. The changes regarding fasting blood glucose(FBG), body weight, serum insulin and pathological changes in β-Islet-cells were observed after continuous 14 days’ medical administration of Astragalus Hypoglycemic Granule. Result: FBG was decreased significantly, the content of serum insulin was increased obviously, the body weight was controlled effectively and the damage of β-Islet-cells was repaired in type 2 diabetic mice of each group after medicine administration. Compared with the mice in model control group ·L-1, serum insulin:(9.69±2.66) mU ·L-1, weight:(20.00±1.18)g], there were significant difference with the mice in high dose of Astragalus Hypoglycemic Granule group ·L-1, serum insulin:(18.98±1.41) mU ·L-1, weight:(26.08±0.63)g] and the mice in metformin hydrochloride group ·L-1, serum insulin:(17.33±1. 23) mU ·L-1, weight:(25.83±0.89)g](P<0.01). There showed statistical significance in the mice of low dose of Astragalus Hypoglycemic Granule group ·L-1,serum insulin:(15.89±1.38)mU ·L-1, weight:(25.69±0.44) g] and the mice in Ginseng-Astragalus Hypoglycemic Granule group ·L-1, serum insulin:(15.80±1.21) mU ·L-1, weight:(22.90±0.74) g](P<0.05). Conclusion: Astragalus Hypoglycemic Granule can significantly decrease fast blood glucose, increase the content of serum insulin and has repair function for β-islet-cells.关键词：type 2 diabetes;Astragalus Hypoglycemic Granule;C57BL / 6 mice;fasting blood glucose;serum insulin;β-islet-cells
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Rehmanniae Radix and Rehmanniae Radix Praeparata on blood glucose and blood lipid in streptozocin-induced diabetic mice. Method: C57 male mice was administrated by intraperitoneal injection of STZ(60 mg ·kg-1) to establish diabetes model. Fresh Rehmannia root of 6, 4, 2 g ·kg-1 and prepared Rehmannia root of the same doses were ig administrated in grouped mice respectively once daily for a week. The effects on blood glucose and blood lipid in diabetic mice were observed accordingly. Result: Fresh Rehmannia root of 6 g ·kg-1 could reduce blood glucose and improve blood lipid in the diabetic mice, compared with the mode group P<0.01.Fresh Rehmannia root of 4 g ·kg-1 and prepared Rehmannia root of 6 g ·kg-1 could reduce blood glucose, and compared with the mode group P<0.05.Fresh Rehmannia root and prepared Rehmannia root showed effects of reducing blood glucose and improving blood lipid in the diabetic mice with dose-effect manner. Conclusion: Fresh Rehmannia root is better for hypoglycemic effect and improving blood lipid in streptozotocin-induced diabetic mice than prepared Rehmannia root.关键词：Rehmanniae Radix;Rehmanniae Radix Praeparata;diabetes;hypoglycemic;improving blood lipid
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of the Three-clearing Hypoglycemic Formula(Sanqing Jiangtang Fang) on the blood glucose and blood lipids in type 2 diabetic rats. Method: Ten rats were selected randomly from 82 male Wistar rats to form the normal group(group C). With high fat forage diet of four weeks, the rest 72 rats were injected of small dose of streptozotocin(STZ) intraperitioneally twice to establish rats model with type 2 diabetes. Based on fasting blood sugar levels, the model rats were randomly divided into group treated with high dose of Three-clearing Hypoglycemic Formula(group SG), group treated with middle dose of Three-clearing Hypoglycemic Formula(group SZ), group treated with low dose of Three-clearing Hypoglycemic Formula(group SD), model control group(group M), group treated with acarbose(group A) and group treated with rosiglitazone(group L). Group SG, group SZ and group SD were administrated with water extracts of Three-clearing Hypoglycemic Formula respectively. Group M was administrated with normal saline. Group A was administrated with acarbose. Compared with group A, group L was administrated with equivalent dosage of rosiglitazone. The sugar tolerances were measured 2 weeks and 4 weeks before and after treatment respectively. Four weeks after treatment, blood was obtained from aorta of rats. blood lipid and hepatic/renal function were tested for further comparisons. Result: ① Four weeks after medicine treatment, there was significant difference between group SD and group M regarding fasting blood glucose levels(P=0.011); ② Four weeks after medicine treatment, there was significant difference in SD group regarding 2 hours-postprandial glucose levels, compared with group SG, group M, group A and group L(P<0.05); ③ Four weeks after medicine treatment, there were no significant difference between each group regarding HDL-c, LDL-c, ALT, AST and crestine levels. Comparing group SZ, group SD, group M and group C, there was significant statistics regarding CHO level(P<0.05). Comparing group SZ, group SD, group A and group C, there was significant difference regarding TG level(P<0.05). Comparing group C with other groups, there was significant difference regarding BUN level(P<0.05). Conclusion: Low dose of Three-clearing Hypoglycemic Formula has better hypoglycemic effect regarding fasting blood glucose and 2 hours-postprandial glucose.关键词：Three-clearing Hypoglycemic Formula(Sanqing Jiangtang Fang);type 2 diabetes;animal model
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发布时间：2024-01-04 - 摘要：Objective: To investigate the inhibitory effects of the serum containing Xiaobanxia plus Fuling formulas on the proliferation of human hepatoma carcinoma HepG2 cell and on the expression of apoptosis associated factors in HepG2. Method: The effects of Xiaobanxia plus Fuling formulas on the proliferation of HepG2 were determined by MTT assay. The cell cyclic retardation was examined by using flowcytometry. Immunohistochemistrywas used to detect the expression of caspase-3 and caspase-9 in the level of protein. Result: MTT showed that Xiaobanxia plus Fuling formulas had an inhibitory effect on the proliferation of HepG2 cells, and the effect was best at 24 h than that at other time. The cell cycle analysis showed that Xiaobanxia plus Fuling formulas increased the percentage of S phase cells, and cyclophosphamide(CTX) reduced the percentage of G2 phase cells in 24 hour. The results of the immunohistochemistry demonstrated the expression of caspase-3 was increased with dose-time dependent. Conclusion: The serum containing Xiaobanxia plus Fuling decoction has a significant inhibitory effect on the proliferation of HepG2.The mechanism of inducing cell apoptosis may be related with increasing in the level of caspase-3.关键词：Xiaobanxia plus Fuling formulas;HepG2;apoptosis;caspase-3
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发布时间：2024-01-04 - 摘要：Objective: To study the effective compounds of the prescripition of Tongmai Oral Liquid(TMOL) and its best compatible proportion. Method: Using method of serological pharmacology, proliferation of glomerular mesangial cell(GMC) was observed to screen the effective compounds of TMOL. Based on the result of screening, using orthogonal experimetnt design, the best compatible proportion between the total polysaccharides and saponins was evaluated. Result: The results showed the total polysaccharide and saponin, which were extracted from the prescription of TMOL could inhibit the proliferation of GMC and the best compatible proportion between total polysaccharide and saponin is 960 mg ∶240 mg. Conclusions: total polysaccharide and total saponin were the chiefly effective compounds and the best compatible proportion was 960 mg ∶240 mg.关键词：Radix Astragali;Radix Et Rhizoma Notoginseng;effective compounds;compatible proportion.
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Fuzhi capsule on the ability of learning and memory and the hippocampus Ach E activity and CHAT content and the content of IL-6,TNF-ɑ in serum in rats with vascular dementia. Method: The rat model was established by blocking bilateral common carotid arteries permanently, and the model rats were randomly classified into two groups, i.e. Fuzhi Capsule group(4.5 g ·kg-1,ig) and model group. Then(3 d, 7 d and 28 d after treatment) active avoid reaction(AAR)rate, hippocampus Ach E activity and CHAT content and the content of IL-6,TNF-ɑ in serum were observed. Such factors were detected as hippocampus Ach E activity and CHAT content in serum after treatment. Result: Cholinergic neurons in the hippocampus were damaged irreversibly after 3 d of ischemia-reperfusion. The Fuzhi Capsule could reduce the damage process in early stage but did not have significant improvement on the damaged cholinergic nerve in the course of treatment. The hippocampus Ach E content was increased significantly after 3 d, then at a low level. Then Fuzhi Capsule increased it in 7 d and 28 d by comparison with the sham operated group, decreased the expression of IL-6 in early ischemia-reperfusion obviously. The expression of TNF-ɑ were abnormal in the early and late inflammation stage. Then treatment of Fuzhi Capsule decreased it obviously comparison with that of the sham operated group. Conclusion: Fuzhi Capsule can improve the ability of learning and memory. This indicates that Fuzhi capsule may mitigate the injury of inflammation and regulate the low level of acetylcholine in hippocampus.关键词：Fuzhi Capsule;vascular dementia;inflammatory factor;acetylcholine system
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发布时间：2024-01-04 - 摘要：Objective: To study the changes in gene expression profiles of gastric carcinoma after treatment of Compound Qinglongyi Capsule and the potential mechanism of it. Method: MTT assay was used to observe the effect of Compound Qinglongyi Capsule at doses of 0.8×10-3,1×10-3,1.2×10-3,1.4×10-3, 1.6×10-3g.L-1 accordingly on proliferation of SGC-7901 cells. The gene expression spectra were tested by gene chip technique, and the databases were analyzed by gene set analysis. Result: MTT results showed that after treated with different doses for 24 h, Compound Qinglongyi Capsule could obviously inhibit the proliferation of SGC-7901 cells in a dose-dependent manner. Compared with the untreated group, expressions of 78 genes were changed, among them 23 gene expression up-regulated, and 55 gene expression down-regulated. Conclusion: Cell proliferation in the carcinoma cell line SGC-7901 can be inhibited effectively by Compound Qinglongyi Capsule.关键词：gastric carcinoma;gene chip;Compound Qinglongyi Capsule
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发布时间：2024-01-04 -
Effect of Jinkui Shenqi Pill on Sperm Quality and Renal Function in Rats Adenine-induced Infertility
摘要：Objective: To observe the influence of Jinkui Shenqi Pill on sperm quality and renal function in rats with adenine-induced infertility. Method: Thirty adult male SD rats were randomly divided into Jinkui Shenqi Pill group, model group and control group. Sperm qualities of experimental rats were detected by automatic sperm quality analyzer, and contents of testosterone(T), estradiol(E2), luteinizing hormone(LH) and follicle-stimulating hormone(FSH) were measured by radioimmunoassay. Contents of BUN and Scr were ested by automatic biochemical analyzer. Renal tissue was observed by optical microscope. Testis was observed by transmission electron microscopy. Result : Jinkui Shenqi Pill could significantly improve symptoms of Shen-yang deficiency in adenine-induced infertility rats, and it could improve the sperm quality, including sperm density, sperm motility and sperm activity. It could increase content of T obviously, lower contents of E2, LH and FSH, lower contents of BUN and Scr. And it can better the damaged kidney tissue in model rats. Conclusion : Jinkui Shenqi pill can improve sperm quality significantly and improve function of kidney. And the mechanism of improving sperm quality may be related to the increase in content of T by stimulating the interstitial cells and lowering level of E2.关键词：adenine;infertility;Jinkui Shenqi Pill;sperm;renal function- 2
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of Dryopteris crassirhizoma on systemic inflammatory response syndrome (SIRS) through establishing the mouse model of SIRS. Method: BALB/c mice were randomly devided into several groups,the normal control group,the LPS model group and the different doses of Dryopteris crassirhizoma extract groups(4.68, 2.34, 1.17 g ·kg-1).The rectal temperature,respiratory rate,wet and dry lung weight ratio,WBC, the level of glucose and lipid metabolism were determined before and after treatment. Result: Intra-peritoneal injection of LPS 6 mg ·kg-1 could significantly lower the body temperature,reduce WBC,make the lung congestion and edema,reduce the level of glucose and lipid metabolism. Osmunda japonica and Matteuccia struthiopteris could raise the low temperature and reduction of WBC caused by LPS,improve the abnormal serum choleterol. Conclusion: O. japonica and M. struthiopteris have marked effect in protecting LPS caused SIRS.关键词：Osmunda japonica;Matteuccia struthiopteris;systemic inflammatory response syndrome;endotoxin
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发布时间：2024-01-04 - 摘要：Objective: To observe the changes of Na+-K+-ATPase activity in the lung tissue and the changes of ultra-structure in alveolar type Ⅱ cells in rats with lung defensive sluggishness syndrome(LDSS) and the regulation of rhubarb on it to explore the material foundation of LDSS and the mechanism of rhubarb in the prevention and treatment of the lung injury. Method: Lipopolysaccharide(LPS)was injected into SD rats to perform lung-defensive sluggishness syndrom,60 rats was randomizly divided into 5 groups:control group, lung-defensive sluggishness group,natural recovery after 5 days group, Rhubarb prevention group(9 g ·kg-1 ·d-1 for 2 days before modle establishment) and rhubarb treatment group(9 g ·kg-1 ·d-1 for 5 days after model establichment).The ultrastructure changes were observed under transmission electron microscope, The homogenate was used to detect the activity of Na+-K+-ATPase with the Na+-K+-ATPase kit. Result: Compared with the normal control group, alveolar type Ⅱ cells Lamellar body vacuoles, endoplasmic reticulum, cytoplasmic swelling and perinuclear extension were showed in the model group. A large number of alveolar type Ⅱ cells Lamellar body vacuoles, endoplasmic reticulum, markedly nuclear condensation were showed in the natural recovery after 5 days group. After the rhubarb intervention, the number of lamellar body of type Ⅱ cells was larger than that in recovery after 5 days group and lung-defensive sluggishness group, especially no enlargement of endoplasmic reticulum. It was showed that the expression of Na+-K+-ATPase activity of lung- defensive sluggishness group was lower than that of control group. However, the expression in the natural recovery after 5 days group was significant higher than control group. Rhubarb could inhibit the abnormal expression of Na+-K+-ATPase activity of lung. Conclusion: The abnormal ultra- structure of alveolar type Ⅱ cells and abnormal expression of Na+-K+-ATPase activity can be showed in lung-defensive sluggishness model and rhubarb can inhibit them,which is one of the important mechanisms for protecting the lung injury in lung-defensive sluggishness syndrome.关键词：acute lung injury;alveolar type Ⅱ cells;Rhubarb;Na+-K+-ATPase;ultra-structure
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Qifu Decoction on heart function of adriamycin-induced cardiac injury in a rat model, and to discuss the mechanism. Method: Heart function was measured by ECG instrument, the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) were detected, and the content of lipid peroxidation product malondialdehyde(MDA) were checked by chemical colorimetric method. Result: Compared with the normal group, the heart rate of the rats in the model group was slowed down, the sum of QRS voltage decreased, Q-T interval extended, all with statistically significance(P<0.05). Arrhythmia appeared in a few individuals. Compared with the model group, the heart rate of the rats in Qifu Decoction group and Chinese herb control group was speeded up, the sum of QRS voltage increased, Q-T interval shortened, all with statistically significance(P<0.05). Compared with the normal group, the activities of SOD and GSH-Px were weakened, and the content of MDA increased in the model group, Qifu Decoction group and Chinese herb control group, all with statistically significance(P<0.05). Compared with the model group, the activities of SOD and GSH-Px were strengthened, and the content of MDA decreased in Qifu Decoction group and Chinese herb control group, all with statistically significance(P<0.05). Conclusion: Qifu Decoction can protect the heart from adriamycin in the rat model. Its mechanism may be related with antioxidant stress.关键词：Qifu decoction;adriamycin;cardio- toxicity;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: By chronic stress and spleen damp-heat to explore the combination of traditional Chinese medicine syndrome with IBS in animal model. Method :Thirty SD rats were randomly divided into 3 groups, namely: the blank group, model groupⅠ(Spleen and Stomach Damp-heat group), model groupⅡ(composite model group), each of 10 rats. Except the blank group, the model groupⅠand group Ⅱ were the spleen damp-heat modeling groups. However group Ⅱ was simultaneously with irritable bowel syndrome by chronic stress methods. By the end, the physiological indices of rats and histological changes were investigated. Result: In the model groups, body weight, body temperature, fecal character and behavior were significant different compared with the control group(P<0.05). However in the intestine there was no significant histological difference. Conclusion: Damp-heat diarrhea-type IBS of rat model is to some extent to human diarrhea-predominant IBS(spleen type) with good repeatability.关键词：traditional Chinese medicine syndrome;damp-heat diarrhea type;irritable bowel syndrome;animal model
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发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-colon cancer effects of total alkaloids from Corptidis Rhizoma. Method: Colon cancer model of Wistar rat was induced by 1-2 dimethylhydrazine(DMH)(40 mg ·g-1 sc)in combination with 1% dextran sodium sulfate solution(DSS)(freely drinking). All rats were randomly divided into 3 groups:control(DMH + DSS + solvent), total alkaloids from Corptidis Rhizoma(Tal)(DMH + DSS + Tal 120 mg ·g-1 and berberine(Ber)(DMH + DSS + Ber 100 mg ·g-1). The drugs were given orally once a day for 5 day per week.The body weight, the number of colon abrrant crypt foci(ACF), the incidence and number of colon cancer in rats, as well as the morphological changes in rat colon tissues were evaluated. Result: Tal significantly improved the dyscrasia induced by DMH+DSS, both of the body weight and general condition were better than that in control group. Tal also significantly inhibited ACF and colon cancer incidence in the rats treated by DMH + DSS for 10 weeks or 20 weeks,which was similar to that of Ber. Conclusion: Tal can inhibit ACF and tumor formation induced by DMH + DSS. This may be directly related to berberine existing in Tal.关键词：total alkaloids from corptidis Rhizoma;berberine;colon cancer;chemoprevention
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-inflammatory effects of the extract of Rubus parvifolius. Method: Butanol fraction from R. parvifolius was obtained for the experiments. Kunming mice and SD rats were used as the animals, and the selection of two dosages was 1.0, 0.5 g ·kg-1 ig for 7 d. Mice ear inflammatory edema was induced with xylene and rat paw edema was created by injection of carrageenan. The mice or rats were divided into groups including negative control (normal saline), positive control (dexamethasone) and the group of the extract from R. parvifolius. The swelling degree of mice ear and rat paw edema, and the swelling inhibition effect caused by the extract of R. parvifolius were observed by using method of volume measurement. Result: The swelling degree of auricle inflammation in mice was reduced by the extract from R. parvifolius compared with that of the control group (P<0.05), and the swelling inhibitive rates were 81.76% and 76.79% in high and low dose group respectively. The foot swelling of rats induced by carrageenan was significantly inhibited by the extract as compared with that in the control group (P<0.05). Conclusion: The extract (total saponins) of R. parvifolius has an obvious anti-inflammatory effect.关键词：butanol fraction of R. parvifolius;anti-inflammatory effect;swelling degree;swelling inhibition rate
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发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-oxidant effect in vitro and in vivo of Houttuyniae Herba polysaccharide on mouse liver,kidney,and heart and brain tissues. Method: Kunming mice were divided into model group, control group and Houttuyniae Herba polysaccharide group. Three Houttuyniae Herba Polysaccharide subgroups were given Houttuyniae Herba polysaccharide for 7 days with doses of 30, 60,120 mg ·kg-1 respectively.The control group was ip injected blend oil,and the other groups was ip given 0.15％ CCl4 mixed with blend oil at 2 h after last administration.After 24 hours blood serum of mice was separated and ALT and AST were determined.The liver index was calculated and MDA in liver homogenate were determined. Free radicals induced by the fenton reaction were determined by absorption at wavelength of 532 nm (A532 nm). Result: Houttuyniae Herba Polysaccharide could alleviate the inflammation in response to experimental liver injury. Different concentrations of Houttuyniae Herba Polysaccharides can reduce ALT and AST in serum (P<0.05) and lower the content of the MDA in tissue (P<0.01). Anti-oxidation experiments of Houttuyniae Herba polysaccharide in vitro shopwed that it inhibited the production of lipid peroxidates and significantly decrease A532nm (P<0.01). Conclusion: Houttuyniae Herba polysaccharides can reduce serum AST,ALT activity, has antioxidant effect in liver, kidney, heart and brain. The antioxidant effect is especially strong for kidney and liver.关键词：Houttuyniae Herba polysaccharide;free radicals;antioxidation
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Jingang wine on the kidney yang and sexual function in rat model and mouse model with deficiency of kidney yang. Method: Rat model and mouse model of deficiency of kidney yang were made by intramuscular injection of hydrocortisone. The animal models were divided into control group, model group, positive group, and Jingang wine group (low doses, middle dose, and high dose, given 10 days). The pharmacological action of the wine was evaluated by body weight,autonomous activities, survival time of animal in hypothermia swimming, prostate weight and seminal vesicle weight, endurance time in hypooxygen, and sex organ accessories in male castrated rats. Result: Compared with the model group, there were obvious difference in the growth rate of weight, prostate weight and seminal vesicle weight, swimming time, and autonomous activities among different dose Jingang wine groups(P<0.05). There was obvious difference in endurance time in hypooxygen condition compared with that of the control group(P<0.05). Compared with the model group, there were obvious differences in erection time and sex organ accessories in the castrated male rats, such as prostate and seminal vesicle and levator ani(P<0.05). Conclusion: Jingang wine would nourish the kidney and support yang, and could improve sexual atrophy and function.关键词：Jingang wine;syndrome of deficiency in kidney yang;nourishing kidney to support yang
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of the decoction of invigorating spleen and regulating Qi(DISRQ) on gastrointestinal motility, motilin(MTL),gastrin(GAS) in rats with functional dyspepsia(FD),exploring the mechanism of the DISRQ for adjusting gastrointestinal dynamics. Method: Seventy-two Wistar rats of SPF class were randomly divided into six groups i.e. normal group, model group,low dose of DISRQ group, middle dose of DISRQ group, high dose of DISRQ group and cispride group(n=12 each, male and female in half). The normal group and model group were given physiological saline intragastrically; while DISRQ groups were administrated with DISRQ at doses of 80,48,16 g ·kg-1 respectively,and cisapride group was given cisapride of 0.005 g ·kg-1. Investigation started from 7 days after modeling and continued for 14 successive days. Gastrointestinal Motility, MTL and GAS in each group were observed. Result: Different doses of DISRQ could improve the function of gastric emptying and promote intestinal propulsion of FD rats in different extent(P<0.05 or 0.01),and the effect of the high dosage showed no significant difference with those in the positive control group(cispride).Compared with those in normal group, the level of plasma MLT and GAS in the model group were remarkably decreased(P<0.05). Different doses of DISRQ could increase the content of MLT and GAS in different extent(P<0.05). There was no significant difference between high dose group of DISRQ and the cisapride group. Conclusion: The therapeutic effect of DISRQ in experimental FD rats might result from improving gastric emptying,promoting the small bowel peristalsis,enhancing the level of plasma MTL and GAS.关键词：decoction of invigorating spleen and regulating Qi;functional dyspepsia;motilin;gastrin
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发布时间：2024-01-04 - 摘要：Objective: To compare the cellular immune function of Liaoning, Jilin and Korean ginseng in immunosuppressed mice. Method: Immunosuppressed model of mouse was induced by subcutaneous injection of hydrocortisone. Three decoctions of ginseng originated from Liaoning, Jilin and Korea were ig given to explore CD3, CD4, CD8 in spleen cell suspension by flow cytometry. Serum IFN-γ level was determined by ELISA assay;and SRBC was used to induce delayed-type hypersensitivity (DTH). Result: The CD3, CD4 and CD8 of spleen cell suspension were increased after each ginseng decoction treatment,Korean ginseng could increase the CD4 and CD4/CD8 ratio for the immunosuppressed mice significantly(P<0.05). Liaoning ginseng had no significant difference compared with that of Jilin ginseng or Korean ginseng. The levels of DTH and IFN-γwere also raised significantly after each decoction treatment(P<0.05), and Liaoning ginseng had no significant difference compared with that of Jilin ginseng or Korean ginseng. Conclusion: Liaoning ginseng decoction can improve cellular immune function on immunosuppressed mice, and Liaoning ginseng had no significant difference compared with Jilin and Korean ginseng.关键词：ginseng decoction;immunosuppression;T-cell subsets;IFN-γ;delayed-type hypersensitivity
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of rutaecarpine (Rut) on human normal hepatic cells (HL7702) and human embryonic kidney cells (HEK293) through in virto experiments.To study the toxicity of rutaecarpine on the vitality of hepatic and renal cells by preliminary compare co-culture system with solo-culture system. Method: ①The influence of rutaecarpine on the activity of hepatic and renal cells through MTT method in solo-culture system and co-culture system was detected.②The shape of cells was observed through inverted microscope.③After giving Rut, the change of ALT,AST,ALP,BUN,Cr and LDH in cultural supernatant of hepatic and renal cells was determined.④After giving Rut by intravenous administration, the influence of Rut on hepatic and renal function and histopathology in mice was investigated. Result: ①The MTT method showed that 2 μg ·mL-1 and 4 μg ·mL-1 of Rut decreased the activity of hepatic and renal cells. The inhibitory action on hepatic cells’ activity was higher than that on renal cell activity under the same concentration of Rut.② In cultural supernatant of hepatic cells, 4 μg ·mL-1 of Rut increased AST, ALP and LDT(P<0.01). ③The animal experiment showed that after giving 10 mg ·kg-1 of Rut to mice by intravenous administration for 7 days, ALP in blood serum was increased notably (P<0.01), while ALT, AST, BUN and Cr had a trend to increase. Histopathological examination revealed that there was an increase in hepatocyte nuclear division in 1/3 mice, meanwhile, basophilic degeneration was only found in one mouse’s kidney. Conclusion: Rut may cause hepatic and renal toxicity. The toxicity to hepatic and kidney cells induced by drugs can be studied by solo-culture system and co-culture system.关键词：rutaecarpine;hepatotoxicity and nephrotoxicity;HL7702 cells;HEK293 cells
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发布时间：2024-01-04 - 摘要：Objective: To oberseve the acute toxicity,anti-fatigue effect and anti-stress effect of Ginseng Tonic Decoction in mice. Method: Mice of KM species were used to test the maximum tolerance dose (MTD)of Ginseng Tonic Decoction. The mouse anti-fatigue test, the mouse acute cerebral ischemia anti-hypoxia test, the mouse anti-hypoxia test and the mouse resistant test to high and low-temperature were used to evaluate anti-stress effect of Ginseng Tonic Decoction at doses of 42.0,21.0 and 10.5 g ·kg-1 ·d-1. Result: the oral MTD of Ginseng Tonic Decoction in mice was>160 g ·kg-1 ·d-1,equal to 76.2 times of human adult’s clinical dose per day. Compared with the control group,mice in Ginseng Tonic Decoction in high dosage group, moderate dosage group and positive control group could prolong the falling time that mice drop from rotating bar because of fatigue;Ginseng Tonic Decoction in high dosage group could prolong the asthmatic time after decapitation and the death time under ambient pressure in mice; Decoction in high dosage group, moderate dosage group and positive control group could prolong the death time under high temperature of 45 ℃ and under low temperature of -5 ℃,there were significant differences(P<0.05 or P<0.01). Conclusion: Tonic Decoction can improve the ability of anti-fatigue, anti-hypoxia and resistance to high and low-temperature. So, the decoction shows anti-stress effects without detectable acute toxicity.关键词：Ginseng Tonic Decoction;mice;acute toxicity test;anti-fatigue effect;anti-stress effect
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发布时间：2024-01-04 - 摘要：Objective: To evaluate realgar suspension’s toxicity and to find out the relationship between the content of soluble arsenic, valence arsenic of realgar suspension come from different batches and the acute toxicity in mice by observing mice’s intoxication reaction and mortality after single-dose intragastric administration with the realgar suspensions. Method: The content of total arsenic, soluble arsenic and valence arsenic from 3 different batches of realgar suspension were tested. The maximum dose level was determined according to the results of preliminary test. Toxicity experiment was designed with ig dose proportion of 0.85.Mice’s intoxication reaction and mortality were observed after administration for 14 days,LD50 and 95% confidence interval were obtained by SPSS software. Result: The total arsenic content of 3 different batches of realgar suspension was 95.5%, 96.0% and 94.8% respectively, soluble arsenic content was 3.32%, 5.23% and 6.73% respectively, valence arsenic content was 1.39%, 3.26% and 4.36% respectively. LD50 obtained from different batches was 2.069 g ·kg-1, 1.319 g ·kg-1 and 1.100 g ·kg-1 respectively. When doubled dosage of LD50 was ig applied, the mice were all dead. Conclusion: The experiment shows that the toxicity of realgar exists in the water soluble components. Soluble arsenic and valence arsenic have strong negative correlation with LD50, which means the higher the content of soluble arsenic and valence arsenic, the higher the toxicity of realgar suspension, while LD50 doesn’t correlate well with total arsenic content.关键词：Realgar;soluble arsenic;valence arsenic;acute toxicity;LD50
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发布时间：2024-01-04 - 摘要：Objective: To investigate the dispositions of Mahuang Tang in rodents by the drug cumulative method and pharmacological effect method and to make a comparison. Method: Taking mortality as the index, mice were injected ip with 19.69 g ·kg-1 Mahuang Tang twice at different time points, and the disposition of Mahuang Tang was determined by the drug cumulative method. Taking antipyretic effects as the index, rats with yeast-induced fever were administered 11.2 g ·kg-1 ig of Mahuang Tang, the disposition was determined by the pharmacological effect method. Pharmacokinetic parameters were determined using DAS software version 2.1.1. Result: The drug cumulative method showed a two-compartment model to describe the disposition, the apparent pharmacokinetic parameters were t1/2β=9.36 h, AUC=46.80 g ·kg-1 ·h, CL=0.32 (g ·kg-1)/(h ·g ·kg-1). The pharmacological effect method applied a one-compartment model to describe the disposition, the apparent pharmacokinetic parameters were t1/2=4.15 h, AUC=55.01 g ·kg-1 ·h, CL=0.27(g ·kg-1)/(h ·g ·kg-1). Conclusion: The pharmacokinetic parameters from two methods are quite different. The drug cumulative method results in a longer elimination half-hour than that of the pharmacological effect method. The biological effect method can be applied to investigate the disposition of traditional medical formulae, predicting pharmacokinetic parameters with different values and meaning apart from the classic pharmacokinetic method.关键词：Mahuang Tang;drug cumulative method;pharmacological effect method;apparent pharmacokinetic parameters
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发布时间：2024-01-04 - 摘要：Objective: To probe into the pharmacodynamic mechanism of Qilian Shupi(QLSP) recipe by way of researching on its treatment on precancerous lesion of Chronic atrophic gastritis(CAG). Method: Seventy-two patients with precancerous lesions of CAG were divided randomly into two groups, 36 of them were treated with QLSP and the other with Wei Fuchun tablet. After three months, the total effective rate, Hp-eradicating rates, gastroscopic images and pathological changes of gastric mucous membranes were observed. Result: The clinical research showed that the total effective rate of QLSP group was 88.9%,while it was 63.9% in Wei Fuchun group; the Hp-eradicating rate of QLSP group was 72%,while it was 43.5% in Wei Fuchun group; QLSP could improve gastroscopic images and pathological results of gastric mucous membrane. Conclusion: Invigorating spleen and tonifying kidney,regulating qi to disperse stagnation,and dispersing accumulation of pathogenic factors to resolve masses are the principles in the management of the precancerous lesion of CAG.关键词：Qilian Shupi recipe;precancerous lesions of chronic atrophic gastritis;invigorating spleen and tonifying kidney;dispersing accumulation of pathogenic factors to resolve masses
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发布时间：2024-01-04 - 摘要：Objective: To observe and learn the current status of vascular cognitive impairment (VCI) in ischemic stroke inpatients. By evaluating the level of cognitive function in ischemic stroke inpatients,and analysing its influential factors. Method: The level of cognitive function in ischemic stroke inpatients was evaluated with the montreal cognitive assessment(MoCA), analysing its influential factors were analysed. Result: Thirty-one cases of VCI were found in 43 cases of ischemic stroke inpatients, accounting for 72.1%, including 13 cases of VaD patients, accounting for 30.23% of all subjects. Which has positive correlation with the level of education. Conclusion: There is a big proportion of cognitive impairment in ischemic stroke patients. It is necessary giving more adequate attention to the status. MoCA is an useful tool in assessing cognitive function evaluation with ischemic stroke inpatients, it can be used for screening VCI patients in ischemic stroke inpatients.关键词：ischemic stroke;cognitive evaluation;MoCA
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发布时间：2024-01-04 - 摘要：Objective: To study the effectiveness of Fuzheng Xiaoliu Tang with Xihuang Capsules to attenuate adverse effect and enhance curative effect for chemotherapy. Method: This randomized controlled trial was carried out in 52 patients with gastric cancer. The patients were divided into test group and control group. The short-term efficiency, KPS score, immune function, hemogram, liver and kidney function were determined before and after treatment. Result : Compare with the control group, Fuzheng Xiaoliu Tang with xihuang capsules could elevate the short-term efficiency, the KPS score, the immune function of the test group. There was no harmful event happened. Conclusion: Fuzheng Xiaoliu Tang with Xihuang Capsules is a safe and effective adjuvant for chemotherapy.关键词：Fuzheng Xiaoliu Tang;Xihuang Capsules;gastric cancer;chemotherapy;attenuate adverse effect and enhance curative effect
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发布时间：2024-01-04 - 摘要：Objective: The aim of our study is to assess the effects of Jianpi Bushen recipe joint metformin in endocrine and biochemical festures of Obese polycysticovariansyndrome(PCOS) patients. Method: Twenty-four obsess PCOS patients were administrated metfomin 500 mg three times daily joint Jianpi Bushen recipe one dose of medicine every day,morning and evening hours service for three months. Twenty obsess PCOS patients were administrated metfomin 500 mg three times for three months only. Baseline characteristics were assessed initially and 3 months after treatment in two groups. Result: Fourteen patients achieved regular menstrual periods in integrative medicine group and eleven patients in Metformin group, a significant reduction occurred in waist-hip ratio(WHR), luteinizing hormone(LH), androgen(T), FINS, IAUC, triglyceride(TG), low density lipoprotein(LDL-c), ApoB (P<0.05-P<0.01) and a significant increasing occurred in ISI (P<0.01) in integrative medicine group. In metformin group, a significant reduction occurred in WHR, LH, T, FINS, IAUC, ApoB (P≤0.05-<0.01) and ISI increased significantly(P<0.05). Conclusion: Three months of Jianpi Bushen recipe joint metformin can reduce LH and T levels, increase insulin sensitivity and reduce the side effects of metformin,espdcially It was superior in improving lipid metabolism than Metformin therapy in obsess PCOS patients.关键词：polycystic ovary syndrome;metformin;Jianpi Bushen recipe
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发布时间：2024-01-04 - 摘要：Objective: To analyze the effectiveness and prognostic factors of antithymus globulin (ATG)/antilymphocyte globulin (ALG) together with herbs of nourishing the kidney in treatment of severe aplastic anemia (SAA). Method: Clinical data of 71 cases with severe aplastic anemia who received ATG/ALD treatment from 1992 to 2009 were analyzed retrospectively. Result: In the 71 patients, 30 were nearly cured (42.3%), 11 were relieved (15.5%), 18 were improved (25.4%) and 12 were invalid (16.9%), The overall effective rate was 83.1%. Among all the factors, we found that neutrophil counts (ANC) more than 0.2×109/L(P=0.0001)and the reticulocyte more than 10×109/L (P=0.001)indicated a higher remission rate, while sex (P=0.320), age (P=0.563), course of disease (P=0.334), platelet count (P=0.621), the percentage of lymphocyte in bone marrow slides less than 60% (P=0.632) had no relation with treatment results. And another research on survival rate found that patients with age over 60 had shorter life span than their counterparts. However, ANC (P=0.288), reticulocyte count (P=0.205), platelet count (P=0.917), the percentage of lymphocyte in bone marrow slides (P=0.914), the course of disease before use ATG/ALG (P=0.093) were not related with survival. Conclusion: ATG/ALG together with Chinese herbs in treatment of severe aplastic anemia was valid and safe, which could also improve clinical curative effect and increase patients’ survival rate. ANC more than 0.2×109/L and the reticulocyte more than 10×109/L would be prognostic factors for efficiency in SAA.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Qixiantang on TNF-α and IL-8 in patients with ulcerative colitis (UC). Method: Fifty patients with UC were divided into therapy group (25 patients treated with Qixiantang) and control group (25 patients treated with sulfasalazine and dexamethasone).TNF-α and IL-8 in serum were detected by ELISA for all patients before and after treatment, and compared with 25 healthy volunteers. Result: Creative effects in therapy group was obviously better than that in control group,the difference was significance (P<0.05).After treatment,compared with control group,therapy group had the lower plasma levels of TNF-α and IL-8 (P<0.05). Conclusion: Qixiantang is effective for patients with UC. The down regulation of the expressions of TNF-α and IL-8 in serum may be one of the therapeutic mechanism.关键词：Qixiantang;ulcerative colitis;TNF-α;IL-8
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发布时间：2024-01-04 - 摘要：Objective: The experience and thoughts from elder and famous of traditional Chinese medicine(TCM) doctors are wealth of TCM. Based on understandings of study and work, in order to inherit the experiences and thoughts from elder famous TCM doctors. The authors compare and analyze advantages and disadvantages of traditional apprenticeship and expert mining methods by modern sciences and technology. Traditional apprenticeship have some advantages to study the thoughts from elder and famous TCM doctors, however, the expert mining methods have some advantages to analyze the experience of treatment and clinical data from elder and famous TCM doctors. A proposal is put forward:Investigative inheriting is an important method to summarize and inherit the experience and thoughts from elder and famous TCM doctors in modern times.关键词：elder and famous of traditional Chinese medicine doctors;traditional apprenticeship;expert mining method;investigative inheriting
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发布时间：2024-01-04 -
Discussion on Prevention and Treatment of Senile Gynecologic Diseases Starting with Regulating Liver
摘要：Because of ovarian failure, the organs and tissues function of elderly women gradually decline. The senile gynecological diseases seriously affect the physical and mental health and quality of life of elderly women. Therefore, the prevention and treatment of senile gynecological diseases is worth the community the widespread attention. The key of prevention and treatment strategies for senile gynecologic diseases is to start with regulating liver. Liver has a close relationship with spleen and kidney. Besides spleen and kidney, the prevention and treatment of senile gynecological diseases especially should attach importance to regulating liver. 'The liver is the congenital dependence of women’ means that the prevention and treatment of women's diseases focuses on regulating liver.On the prevention and treatment of senile gynecological diseases regulating liver should start well in advance.关键词：elderly women;gynecologic diseases;prevention and treatment;regulating liver- 2
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发布时间：2024-01-04 - 摘要：Through experiments, we find, in all kinds of toxins of spiders, many erzymes and some neurotoxins that have selective effect on the nervous system of mammals and insects. Selenocosmia huwena is a new spider of theraphosidae found in Yunan and Guangxi, whose toxin contains SLH-1, four neurotoxins (namely HWTX-I,HWTX-Ⅱ,HWTX-V and HWTX-XI) and some complicated mixtures consisted of polypeptides (mainly those of mulecular 2×103-9×103), proteins and low-molecular-weight substances. With many activated and physiological effects, the toxin of selenocosmia huwena will be used intensively in curing epilepsy, cancers, cerebral hemorrhage, diabetes and hepatitis.关键词：selenocosmia huwena;toxin;ingredient;application research
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发布时间：2024-01-04 - 摘要：FT Raman spectroscopy is a new analytical techniques in recent 20 years. Advantages of FT Raman spectroscopy is fast, simple and accurate and no damage to the samples and so on. Rich resources of Chinese herbal medicine in china, It is key to enhance the management of quality of traditional Chinese medicine for modernization of Chinese medicine. This paper review the application of the Raman spectroscopy in the identification of Chinese herbal medicines, analysis of active ingredients and quality control of Chinese medicine.关键词：Raman spectroscopy;Chinese medicine;identification;quality control;applications
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