最新刊期
卷 16 ， 期 17 ， 2010
- 摘要：ObJective: To investigate the drug-releasing efficiency of triptolide microemulsion gel in vitro. Method: Franz diffusion cell was used to investigate the percutaneous absorption of the triptolide with semipermeable membrane and mice skin as the percutaneous barrier. Result: The experimental equation fitting for drug release characteristics of triptolide microemulsion gel was the first-order model in vitro, and the percutaneous speed was 2.6 μg ·cm-2 ·h-1. Conclusion: The transdermal speed of triptolide was less than the drug-releasing speed in triptolide microemulsion gel; triptolide microemulsion gel was the controlled releasing preparation.关键词：microemulsion gel;percutaneous permeability;the drug release rate
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发布时间：2024-01-04 - 摘要： ObJective: To investigate the purification processes of Scutellaria from Sanhuang spray with macroporous adsorption resin. Method: The content of baicalin was measured with HPLC. The adsorption rate and desorption rate were measured to evaluate the optimal macroporous adsorption resin and the optimal purification conditions. Result: The optimal macroporous adsorption resin was SP-825. The optimal purification condition was as follows: the sample concentration was 0.2 g ·mL-1; the diameter height ratio was 1 ∶7; the sample volume was 2 BV; the eluant was 70% ethanol; the elution flow rate was 3 BV ·h-1; the ethanol consumption was 6 BV. Conclusion: Purification process of the extract of Scutellaria and Gardenia with SP-825 macroporous adsorption resin was stable and feasible.关键词：macroporous adsorption resin;baicalin;purification
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发布时间：2024-01-04 - 摘要： ObJective: To study the drug release characteristics of Shuanghuanglian in-situ gel in vitro. Method: The phosphate buffer of pH 7.4 was taken as the releasing mediator, the content of the chlorogenic acid, baicalin and phillyrin in the releasing mediator was determined by HPLC, the drug release charactersitics of the Shuanghuanglian in-situ gel was studied by Franz diffusion cell method. Result: The releasing curve of the the chlorogenic acid, baicalin and phillyrin followed the Ritger-Peppas equation kinetics, but the releasing rate of the chlorogenic acid was faster than that of the baicalin and phillyrin. Conclusion: the drug release mechanism of Shuanghuanglian in-situ was non-fickian diffusion release.关键词：Shuanghuanglian in-situ gel;drug release in vitro;HPLC;Franz diffusion cell
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发布时间：2024-01-04 - 摘要：ObJective: To establish a preparation method of Niuhuang Shangqing pellets. Method: Optimization of yield and roundness was prepared by extrusion-spheronization process and researched the dissolution of pellets in vitro. Result: Blend the extraction with MCC, and add adequate water. Squeeze with 30 r ·min-1 and roll 6 min with the rate 50 Hz, drying and screening. Conclusion: The pellets had a good appearance and stable quality.关键词：Niuhuang Shangqing pellets;preparation;extrusion-spheronization
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发布时间：2024-01-04 - 摘要：ObJective: To explore the possibility for application of the ultrafiltration technology using polysulfone (PS) hollow fiber membrane to purify water-extraction of Lonicera and optimize the preparation procedure. Method: The PS membrane of different aperture 50 000, 10 000 and 6 000 were studied on the applicability with water-extraction of Lonicera. The proper molecular weight retention was optimized with removal of liquid impurities and the transformation ratio of luteolin and chlorogenic acid as index, while the proper ultrafiltration condition was optimized by orthogonal tests. Result: The PS membrane with 10 000 interception molecular weight had better separation efficiency and the preferred operation parameters were 0.1 MPa of pressure, 30 ℃ of liquid temperature, and 2.5 L ·min-1 of liquid speed. Conclusion: The preferred aperture of PS membrane and the operati on conditions are feasible for the purification of water-extraction of Lonicera, which can effectively improve the production efficiency.关键词：polysulfone hollow fiber membrane;ultrafiltration;chlorogenic acid
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发布时间：2024-01-04 - 摘要：ObJective: To make the optimum process conditions of extraction for Fulin granules. Method: Orthogonal design was used for the optimum extraction of Fulin granules with paeoniflorin and dry-paste as marker. Result: Optimum processing condition was as follows: water reflux-extraction three times with 10 folds water for 2 h, 8 folds water for 1 h and 8 folds water for 1 h respectively. Conclusion: This method is simple and can be applied rightly in mass production.关键词：Fulin granules;extraction process;Orthogonal Design
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发布时间：2024-01-04 - 摘要：ObJective: To study the formation technology of YangJing capsule. Method: Moisture absorption percentage and particles qualified rate were used as index to screen proper excipient and the best dry granulating technology conditions respectively. The inspected angle of repose, bulk density and the critical humidity were also investigated. Result: Dextrin as excipients had a good anti-wet performance. The best method to make granula was as follows: rollers speed at 7 r·min-1, transmission speed at 40 r·min-1, 0.4 mm as the distance of two rollers and 80% ethanol as wet agent. Conclusion: The molding technology was reasonable, and can provide reliable basis for production.关键词：Yangjing capsule;molding technics;dry granulating
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发布时间：2024-01-04 - 摘要：ObJective: To optimize the formulation of Huanglian-Jiedu bioadhesive microspheres. Method: The optimum formulation was investigated by central composite design with five responses ie. yield, entrapment efficiency of three effective ingredients and mucoadhesive property. The data were imitated using second-order polynomial equation, and the numerical optimization was performed. Result: Prepare microspheres with optimum formulation, and the responses approached the prediction at the optimum. Conclusion: The performulation and preparation of microspheres were reasonable and reliable.关键词：central composite design;Huanglian-jiedu;bioadhesive microspheres;optimum formulation
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发布时间：2024-01-04 - 摘要：ObJective: To study the preparation of polysaccharide capsule from corn stigma. Method: Single factor test was used for screening the optimum condition for preparation of corn stigma polysaccharide capsule, including the concentration and consumption of wetting agent, drying temperature and time, to establish a determination method. Result: The preparation technology established was simple and stable. Conclusion: The method established can promote the utilization of corn stigma resource.关键词：polysaccharide from corn stigma;capsule preparation
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发布时间：2024-01-04 - 摘要：ObJective: To optimize the process of extracting effective constituents from Radix Salvia by central composite design/response surface methodology. Method: The effects of influence factors such as solvent fold, ethanol concentration and extract time on extraction rate of tanshinone Ⅱa and salvianolic acid were investigated by using box-behnken design and response surface method. The data were imitated using multi-linear equation and second-order polynomial equation. Result: Multiple correlation coefficients from second-order polynomial equation were prior to those from multi-linear equation. The optimum condition of extraction process was 9 fold solvent, 1.5 hours for decoction, and 3 times for extraction; the ethanol concentration was 60%. Bias between observed and predicted values was less than 3%. Conclusion: The optimum model was highly predictive.关键词：Radix Salvia;central composite design;response surface methodology;extraction process
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发布时间：2024-01-04 - 摘要：ObJective: Preparation of microcapsules of rutin and its quality evaluation. Method: Gelatin w,as used as capsule material and the rutin microcapsules were prepared by a single pool method.Optimization was performed by orthogonal design.Its preparation,encapsulation efficiency and microcapsule particle size,drug loading,in vitro release were also studied. Result: The optimum condition of rutin microcapsules prepared by this method was as follows:3% gelatin was used with capsule material and heart sac ratio of 1 ∶2;sacculation temperature was at 60 ℃ with stirring speed of 400 r ·min-1.The average encapsulation efficiency of rutin microcapsules obtained by the method was 75.24%.The particle size distribution was 76.40% in 25-35 μm,with an average drug loading of 32.04%.In vitro release of 30.48% reached in 0.5 h and the cumulative release in vitro reached 90% in 12 h. Conclusion: Microcapsules containing drugs prepared by the optimum conditions had shown good reproducibility,process stability,and good sustained action by in vitro release test.关键词：rutin;microcapsules;orthogonal design;encapsulation efficiency
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发布时间：2024-01-04 - 摘要：ObJective: To study the optimal technologies for the purification of total flavones from Valeriana Jatamansi with macroporous resin. Method: Taking the static elution ratio and absorption ratio as indexes, the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index. Result: The HPD600 macroporous resin among five types of resins was found with the best separating efficiency, by which static absorption capacity and desorption ratio could reach 131.11 mg ·g-1 and 91.69%, respectively. The best result of dynamic absorption was based on the followings: sample concentration 3.42 g ·L-1,feed rate 2.4 BV ·h-1, sample capacity 25.64 mg ·g-1, elution flow rate 2.4 BV ·h-1.After eluted with 3BV of distilled water and 4 BV of 80% ethanol, the yield of total flavones was 80.36% and the purity was 41.20%. Conclusion: With definite technical process, the HPD600 macroporous resin could be used to purify the total flavonoids of V. Jatamansi.关键词：Valeriana jatamansi;total flavonoids;macroporous resins;purification
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发布时间：2024-01-04 - 摘要：ObJective: To optimize the best semi-bionic extraction condition of liposoluble and water-solubility ingredients from Salvia miltiorrhiza Bge. Method: The semi-bionic extraction condition was optimized by making the extraction ratios of tanshinoneⅡA, salvianolic acid B, total phenolic acid and dried extract weight as indices. The experiment was designed by a orthogonal test. Result: Optimum extraction condition was: alcohol 70%, time 2 h, stock ratio 1 ∶18 (g ·mL-1, mass: volume). Conclusion: The method of semi-bionic extraction-70% ethanol extractions is scientific and rational.关键词：orthogonal test;Salvia miltiorrhiza Bge.;semi-bionic;extraction method
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发布时间：2024-01-04 - 摘要：ObJective: To establish an HPLC method for the content determination of gastrodine and guanosine in Banxia Baizhu Tianmatang. Method: The Platisil ODS-C18 column was used. The mobile phases consisted of water and acetonitrile with gradient elution. The flow rate was 0.65 mL ·min-1 and column temperature was at 25 ℃.The detection wavelength was at 260 nm. Result: Gastrodine and guanosine were linear in the range of 0.007-0.35 g ·L-1 (r=0.999 9) and 0.000 32-0.016 g ·L-1 (r=0.999 9) respectively. The average recoveries were 99.7% and 100.6% with the RSD 1.9% and 2.0% (n=9) respectively. Conclusion: The method was accurate and quick, and can be used for quality control of Compound Pinelliae and Atractylodis Macrocephalae and Grastrodiae Decoction.关键词：Banxia Baizhu Tianmatang;gastrodine;guanosine;HPLC
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发布时间：2024-01-04 - 摘要：ObJective: To study and compare the components content of Terminalia chebula Retz. and T. chebula Retz. Var. tomentella Kurt, lay the foundation for improving its quality standard. Method: Character identification, TLC and HPLC were used to determine the content of gallic acid from them. Result: According to the determination of the 13 batches of T. chebula Retz. and the 11 batches of T. chebula Retz. Var. tomentella Kurt., the content of gallic acid in T. chebula Retz. was significantly higher than that in T. chebula Retz. var. tomentella Kurt., and the highest content was up to 7 times. The results of identification did also show large gap between them. Conclusion: The methods established in this article to determine the content of gallic acid can be used for the quality control of T. chebula Retz. and the result is reliable and reproducible. The method of TLC has a good specificity, which can well distinguish the component differences between them. The purpose of identification can be achieved.关键词：Terminalia chebula Retz.;Terminalia chebula Retz. var. tomentella Kurt.;TLC;content determination;quality evaluation
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发布时间：2024-01-04 - 摘要：ObJective: To establish an HPCE method for determining the fingerprint of Tongsaimai pellet. Method :The buffer was composed of 40 mmol ·L-1borax (pH 9.18)-methanol (90 ∶10).CE was performed on a 75 μm×56 cm (effective length is 50.0 cm)fused silica capillary.Separation voltage was 25 kV,sampling pressure was 5 kPa,sampling time was 2.0 s,the temperature was maintained at 25 ℃,and detected at 254 nm. Result :Based on the fingerprints of 10 batches Tongsaimai pellet, an average electropherogram was used as the standard fingerprint, among which there were 16 characteristic peaks. Conclusion :The fingerprint analysis can be used for assessing the quality consistency of Tongsaimai pellet from batch to batch.关键词：Tongsaimai pellet;fingerprint;HPCE
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发布时间：2024-01-04 - 摘要：ObJective: To study the change of the content of active ingredient in Chinese material medicine. Method: Seven kinds of different compatibility of Dachengqi dceoctions were made when Rhei Radix et Rhizoma was decocted with other three drugs (Magnoliae Officinalis Cortex,Aurantii Fructus Immaturus and Natrii Sulfas),then the quantitative analysis of naringin and hesperidin was determined by HPLC. Result: The flavonoid content was influenced by different compatibility of Dachengqi decoction. The contents of naringin and hesperidin were 4.45% and 1.16% in the traditional decoction, of which higher than those of in other decoctions. The linear range of naringin and hesperidin showed a good correlation from 0.168 5 to 1.684 8 μg(r=0.999 8),and from 0.022 8 to 0.228 0 μg(r=0.999 5),respectively. Their average recoveries were 98.85%(RSD=1.36%) and 98.20%(RSD=2.26%),respectively. Conclusion: The flavonoid content was increased when all drugs were decocted. The HPLC method was well specified for determinantion of the contents of naringin and hesperidin,which could be applied to the quality control and active ingredient determination of compatibility of Dachengqi decoction.关键词：Dachengqi Decoction;compatibility;naringin;hesperidin
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发布时间：2024-01-04 - 摘要：ObJective: To determine the content of rutin and quercetin in the tartary buckwheat extract, so as to appraise its quality. Method: The chromatographic conditions were as follows: with the Welchrom-C18 (4.6 mm×150 mm, 5 μm) as the chromatographic column, methanol-0.2% formic acid solution (50:50) as the mobile phase, 280 nm as the detection wavelength, 30 ℃ as the column temperature, 1.0 mL ·min-1 as the flow rate. Result: The linear range for rutin was 1.56-100.00 mg[DK]·L-1 and the linear range for quercetin was 1.52-97.00 mg[DK]·L-1. The average recovery for rutin and quercetin was 98.49% (RSD 1.25%,n=5) and 97.87% (RSD 1.69%,n=5) respectively. Conclusion: Rutin and quercetin were 18.97%±1.43% and 5.03%±0.42% respectively determined by the method applied.关键词：tartary buckwheat extract;rutin;quercetin;HPLC
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发布时间：2024-01-04 - 摘要：ObJective: To determine fast the content of paeonol in Liuwei Dihuang pills from different manufacturers by near-infrared spectroscopy (NIRS). Method: Near-infrared spectra of different batch of Liuwei Dihuang pills from different manufacturers were collected. Based on the reference values measured with HPLC, the quantitative calibration model of paeonol was built with near-Infrared spectrometry combined with partial least squares (PLS). Result: The internal cross validation proved that the correlation coefficient (R2) was 0.952 1 and the root-mean-square error of cross-validation (RMSECV) was 0.020 93; By the verification of the external validation samples, the root-mean-square error of prediction (RMSEP) was 0.099 7 and the average recovery was 100.38%. Conclusion: The predicting model was accurate and credible. The proposed method could be used in predicting the content of paeonol in Liuwei Dihuang pills. The simple, fast and non-destructive NIRS combined with PLS can provide a new approach to determine rapidly the content of paeonol in large numbers of samples.关键词：near-infrared spectroscopy;Liuwei Dihuang pills;paeonol;partial least square
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发布时间：2024-01-04 - 摘要：ObJective: To establish a method for determination of plumbagin in ZiJintougu sprays. Method: The column was Elipse-XDB-C18 (4.6 mm×150 mm, 5 μm) and the mixture of methanol-water (70 ∶30) was used as mobile phase; the detection wavelength was at 406 nm. Result: The linearity was in the range of 0.046-0.410 μg (r=0.999 9). The average recovery was 99.96% with RSD of 1.86%. Conclusion: The method was simple, rapid and accurate, and suitable for the quality control of ZiJintougu sprays.关键词：HPLC;Zijintougu sprays;plumbagin
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发布时间：2024-01-04 - 摘要：ObJective: To study antimicrobial activity against SA, MRSA and ESBLs by Musa basJoo rhizome and flower. Method: Disc diffusion method was applied to determine the MIC value. Result: MIC in the petroleum ether extracts of the rhizome against SA, MRSA and ESBLs was 31.25 μg ·disc-1. MIC in n-BuOH extract against MRSA and ESBLs was 250 μg ·disc-1. MIC in the petroleum ether extracts of the flower against SA, MRSA and ESBLs was 125 μg ·disc-1. The order of antimicrobial activity was petroleum ether extracts of rhizome > petroleum ether extracts of flower>n-BuOH extract of rhizome. Conclusion: Petroleum ether extracts of rhizome and flower were active fraction.关键词：Musa basJoo;antimicrobial activity;Staphlococcus aureus;MRSA;ESBLs
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发布时间：2024-01-04 - 摘要：ObJective: To establish a method for the determination of schisandrin and schisandrin B in Wuweiziyou soft capsule. Method: An HPLC method was set up with Kromasil C18 (4.6 mm×200 mm, 5 μm) column. The Mobile phase was H2O (A) and MeOH (B). Gradient elution was performed with 67% B from 0-10 min, 67%-78% B from 10-15 min, 78% B from 15-35 min, and 78%-67% B from 35-38 min, 67% B from 38-43 min. The flow rate was 1.0 mL ·min-1, the column temperature was at 30 ℃ and detection wave length was at 254 nm. Result: The linear ranges of schisandrin and schisandrin B were within 98.80-989.0 g (r=0.999 8, n=6) and 38.8-388.0 g (r=0.999 8,n=6), and the average recoveries were 102.05% and 100.98% respectively. Conclusion: This method was easy, sensitive, specific and accurate for the determination of schisandrin and schisandrin B in Wuweiziyou soft capsule.关键词：Wuweiziyou soft capsule;schisandrin;schisandrin B;HPLC
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发布时间：2024-01-04 - 摘要：ObJective: To establish the analysis method for gypsum and calcined gypsum by XRPD. Method: XRPD fingerprints and strong lines of gypsum and calcined gypsum were analyzed. Result: Sixteen specimens of gypsum, 5 specimens of calcined gypsum and gypsum contained preparation Qingyanliuweisan were analyzed and identified by XRPD. XRD strong lines of gypsum and calcined gypsum were acquired. Conclusion: The method established can be used for identification of gypsum and calcined gypsum because of different XRPD fingerprints.关键词：gypsum;calcined gypsum;X-ray powder diffraction;Qingyanliuweisan
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发布时间：2024-01-04 - 摘要：ObJective: To establish the quality standards for HuoxueJiangmei powder. Method: TLC method was used for identification of Fructus Schisandra Chinensis,Flos Carthami and Radix et Rhizoma Salviae Miltiorrhizae;and HPLC methods was used for the contents of tanshinoneⅡA in Radix et Rhizoma Salviae Miltiorrhizae in HuoxueJiangmei powder. Result: TLC method was simple, specific reproducible; HPLC method established was simply and accurate. TanshinoneⅡA was linear in the rang of 0.026 67-0.213 4 μg. The average recovery was 101.19% (RSD 1.18%). Conclusion: This quality standard can control the quality of HuoxueJiangmei Powder effectively.关键词：Huoxuejiangmei powder;TLC;HPLC;tanshinoneⅡA
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发布时间：2024-01-04 - 摘要：ObJective: To establish an HPLC method for separation and determination of rhein, emodin and chrysophanol in Dahuangtongqi oral liquid. Method: The chromatographic condition was as follows: Inertsil ODS-SP (4.6 mm×150 mm, 5 μm) column was used with the mobile phase of methanol-0.1% phosphate acid (72 ∶28); the flow rate was 1.0 mL ·min-1, column temperature was at 25 ℃ and detection wavelength was at 440 nm. Result: Rhein, emodin and chrysophanol in Dahuangtongqi Oral Liquid were linear in the range of 4.2-50.4 (r=0.999 9), 3.9-46.8 (r=0.999 7),4.2-50.4 mg ·L-1 (r=0.999 9) respectively. The average recovery of rhein, emodin and chrysophanol was 97.68%, 97.36%, 97.27%, respectively. Conclusion: This method was simple. The results were accurate and reproducible. It can be used in the quality control of Dahuangtongqi oral liquid.关键词：Dahuangtongqi oral liquid;rhein;emodin;chrysophanol
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发布时间：2024-01-04 - 摘要：ObJective: To investigate the chemical constituents in leaves of Lonicera Japonica. Method: Column chromatography with macroporous resin and versamide,TLC and prep-HPLC were used to isolate compounds. Physicochemical constants and spectroscopic analysis were employed for the structural elucidation. Result: Six compounds were isolated and elucidated as lonicerin(Ⅰ),3,5-dicaffeoylquinic acid(Ⅱ), 4,5-dicaffeoylquinic acid(Ⅲ), 1,5-dicaffeoylquinic acid(Ⅳ),β-sitosterol(Ⅴ), β-daucosterol (Ⅵ), Conclusion: CompoundsⅡ,compounds Ⅲ,compounds Ⅳ are isolated from Leaves of L. Japonica for the first time.关键词：leaves of Lonicera japonica;chemical constituents;identif ication
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发布时间：2024-01-04 - 摘要：ObJective: To study on the chemical constituents of Desmodium heterocarpon. Method: The compounds were isolated by column chromatography with silica gel and Sephadex LH-20. The structures were identified on the basis of spectral analysis. Result: Eight compounds were obtained and identified as betulic acid (1), friedelin (2), n-hexadecanoic acid (3), β-sitosterol (4), (Z, Z)-9,12-octadecadienoic acid (5), 1-hentetracontanol (6) octacosane (7) and vitexin(8). Conclusion: All these compounds were obtained from this plant for the first time.关键词：Leguminosae;Desmodium;Desmodium heterocarpon;chemical constituents
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发布时间：2024-01-04 - 摘要：ObJective: As the research obJect of chlorogenic acid, using X-ray powder diffraction analysis of its crystal structure. Method: In use of XPRD at step of 0.01° scan and get a high-resolution diffraction pattern. Result: Through indexing to identify the crystal system and the space group, then used monte carlo simulated annealing method and rietveld refinement to determine the three-dimensional crystal structure and fractional coordinates of each atom in crystal cell. Conclusion: Powder crystal structure of chlorogenic acid is orthorhombic, space group Pna21 with unit cell dimensions a=36.132 , b=11.709 , c=8.152 , ɑ=90°, β=90°, r=90°, V=3 448.86 3, Z=4.关键词：chlorogenic acid;X-ray powder diffraction;crystal structure
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发布时间：2024-01-04 - 摘要：ObJective: To establish a method of evaluating reaction abilities of flavonolds with Fe(Ⅱ) with 9 flavonoids have been investigated in ethanol solution at 20 ℃ by UV-Visible spectroscopy. Result: Based on the changes of the UV-Vis spectra, we could determine that 7 flavonoids (quercetin, myricetin, rutin, kaempferol, baicalin. baicalein and luteolin) occurred complesx reaction, due to the bathochromic shift of the wavelength band. On the other hand. no bathochromic shift as indication for complexation of catechin and chrysin could be observed under the conditions we had chosen. The kinetics of complex formation were investigated with the ligand in zero-order by the presence of Fe(Ⅱ). The reation rate constants in turn were as follows:myricetin>quercetin>baicalein>kaempferol>baicalin>rutin>luteolin. Conclusion: The strongest chelating power is the pyrogallol hydroxyl in ring B, and the pyrocatechol hydroxyl ranks second, a single phenolic hydroxyl relatively weak. When flavonoid aglycones transform into glycosides. their complex capacity will decrease significantly. The system investigated can be considered as a simplified method to evaluate the complex ability between flavonoids and Fe(Ⅱ).关键词：flavonoids;Fe(Ⅱ);UV-Vis spectroscopy;complexation;kinetics
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发布时间：2024-01-04 - 摘要：ObJective: To ananlysis the volatile oil from root of Alpinia stachyoides. Method: The volatile oil was extracted by steam distillation and analysed by GC-MS. Result: Through comparison with mass spectra database,28 components were resolated and identified, The main componets are β-pinene(8.37%),camphor(8.07%),and myrtle enol(6.70%)et al. Conclusion: Analysis can provida evidence for quality control and integrated usage of root of Alponia stachyoides Hance.关键词：root of Alpinia stachyoides;volatile oil;gas chromatography spectrometry(GC-MS)
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发布时间：2024-01-04 - 摘要：ObJective: To study the chemical constituents and antioxidant activity of the essential oils from Cinnamomum burmannii in Guangxi. Method: The essential oils were extracted by steam distillation, and then the constituents were separated and identified by GC-MS. The scavenging activities on DPPH radical, ABTS+radical and reducing power were detected by UV-Vis spectrophotometry. Result: 137 compounds were isolated and 61 compounds were identified in the leaves that composed about 93.58% of the total essential oils. The maximal scavenging rate on DPPH and ABTS+ radical reached to 21.71% and 58.89%, respectively. And these oils had weaker reductive capability than BHT. Conclusion: The principal chemical constituents of the essential oils are caryophyllene (21.71%), eucalyptol (18.22%),guaiol (7.52%), (+)-α-terpineol (7.06%), (-)-β-pinene (3.57%), γ-eudesmol (3.33%), bulnesol (3.16%), (Z)-nerolidol (3.16%), elemol (2.67%), α-caryophyllene (2.22%), (1S)-α-pinene (1.9%), (-)-terpinen-4-ol (1.8%), (+)-ledene (1.35%), caryophyllene oxide (1.29%), and γ-terpinen (1.05%). These oils possessed good scavenging activities on ABTS+ radical, but showed low activities on scavenging DPPH radical and reducing power. And the antioxidant activities of these oils were concentration-dependent.关键词：Cinnamomum burmannii;essential oils;antioxidant activity;GC-MS
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发布时间：2024-01-04 - 摘要：ObJective: To contrast ingredients of rehmannia dride rhizome with different disct. Method: Carbohydrate was extracted by supersound and water used for solvent, was separated by NH2 chromatographic column and acetonitrile-water(72 ∶28) for mobile phase at 1.0 mL ·min-1,was detected by refraction detector and was assayed by high-performance liquid chromatography. Catalpinoside was extracted by supersound and methanol used for solvent, was separated by C18 chromatographic column and methanol-0.1%phosphoric acid solution at 1.0 mL ·min-1,was detected at λ=210 nm and was assayed by high-performance liquid chromatography. Result :Carbohydrate ingredients of rehmannia dride rhizome and prepared rhizome of rehmannia are conformity,but content are different. Content of catalpinoside have striking difference. Conclusion: The method is good in the aspect of reproducibility and sensitivity.It can be applied to contrast for the ingredients of Rehmannia dride rhizome and prepared rhizome of rehmannia.关键词：place of production;rehmannia root;oligosaccharide;catalpol;high-performance liquid chromatography
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发布时间：2024-01-04 - 摘要：ObJective: To study the pharmacodynamic actions of Jingangteng's effective ingredients(JEIS) on chronic pelvic inflammation disease (CPID)and to provide experimental evidence for Jingangteng's further development. Method: The models of CPID were made by inJection of brei admixed with phenol and tragacanth into the uterus of rats,and the inhibitory effect on CPID was observed. The severity of oedema in inflammation was observed to study the anti-inflammatory effects of JEIS. The analgesic effect of the drug was studied with pain model of mice induced by acetic acid, the PGE2 contents in the uterus in CPID rats were measured by enzyme-linked immunosorbent assay (ELISA). Result: JEIS could obviously inhibit the inflammation of uterus in rats with CPID. The number of stretching induced by acetic acid was reduced and the pain threshold of mice was increased by JEIS. JEIS also had anti-inflammatory activity against xylene-induced mouse ear swelling and albumen-induced rat paw edema. JEIS could significantly decrease the level of PGE2, in the uterus of CPID rats. Conclusion: JEIS has significant analgesic and anti-inflammatory activities, and decreasing of PGE2 may be one of the anti-inflammation mechanisms of JEIS.关键词：Jingangteng;effective ingredients;chronic pelvic inflammation;anti-inflammation;analgesia
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发布时间：2024-01-04 - 摘要：ObJective: To determine the concentration of sinomenine and triptolide in nude mouse hypodermis. Method: Microdialysis coupled with high-performance liquid chromatography was developed for on-line determination of the in vivo concentration of sample, when the perfusate was 30% ethanol/physiological saline solution.The Diamonsil-C18 column (4.6 mm×250 mm,5 μm)was used and the mobile phase consisted of methanol ∶buffer phosphate(pH 9.0)(55 ∶45).The detect wave length was 225 nm.The data obtained after being calculated using the relative recovery in vivo were dealed with WINNONLIN 5.2 programe,while retro-dialysis method was used to study relative recovery in vivo . Result: The peak time of sinomenine and triptolide were 14 h and 10 h, peak concentrations were 30.15 μg ·mL-1 and 3.31 μg ·mL-1 respectively. Conclusion: The method can be used to determine in vivo and real time concentration of sinomenine and triptolide in the skin dynamically. It is suitable for the pharmacokinetics study of sinomenine and triptolide at the same time.关键词：sinomenine;triptolide;microdialysis;pharmacokinetics;HPLC
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发布时间：2024-01-04 - 摘要：ObJective: To observe histopathological changes in renal arteriolar sclerosis for effect of Compound Calculus Bovis Depressurization Formula (CCBDF) on spontaneous hypertensive rats (SHR). Method: SHR of 14-week was used in the study. The rats were treated with CCBDF through intragastric administration for 8 weeks, then histopathological changes in renal arteriole were investigated. Result: Compared with the model group, blood pressure in treated groups ·kg-1(crude drug powder)and 0.6 g ·kg-1(tablet)] were all reduced significantly(P<0.01); the numbers of SHR renal arteriolar sclerosis were decreased, accounting for 20%,20%,30%,40% of total animal number respectively(model group 80%); the number of vessels diseased(NVD) was reduced; and the extent of lesion was lessened. The pathological grades of the four treated groups was 5,5,7,7 score respectively(model group 20 score).Rank sum test (K-S)showed there was significant difference between treated groups(high and middle doses)and model group(P<0.05). Conclusion: Adult SHR can develop renal arteriolar sclerosis and CCBDF has protective effect on SHR renal arteriolar sclerosis as it reduces their hypertension.关键词：spontaneous hypertensive rat;Compound Calculus Bovis Depressurization Formula;hypertension;renal arteriolar sclerosis;pathological changes
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发布时间：2024-01-04 - 摘要：ObJective: To study the protection effects of Swertia pseudochinensis Hara extract on experimental liver inJury. Method: Mouse model of acute liver inJury was established by ip inJection of 0.1% carbon tetrachloride (CCl4) oil solution (0.01 mL ·g-1), and D -Galactosamine ( D -Gal, 800 mg ·kg-1) respectively. Rat model of acute Jaundice was developed by ip inJection of α-naphthyl isothiocyanate oil solution (ANIT,100 mg ·kg-1). Several doses of S. pseudochinensis Hara extract were ig administrated to the models. Activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL), direct bilirubin (DBIL) Content in serum were determined. Bile flow in rats and pathological changes in liver were also investigated. Result: S. pseudochinensis Hara extract at the concentration of 92.6 mg ·kg-1inhibitted the acute liver inJury induced by CCl4 and D -Gal in mice. It decreased the increasing level of transaminases caused by ANIT in rat Jaundice model, and reduced the liver degeneration and the number and extent of necrosis in the model animals. The extract also increased the bile flow rate in the rat Jaundice model. Conclusion: S. pseudochinensis Hara extract has the efficacy of protecting liver from inJury to a certain extent.关键词：Swertia pseudochinensis Hara;carbon tetrachloride(CCl4);D -galactosamine;α-naphthyl isothiocyanate;liver injury
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发布时间：2024-01-04 - 摘要：ObJective: To explore the damage of intestinal barrier in the rats model of dampness blocking spleen and stomach syndrome and the functional recovery of Pingwei powder for it. Method: SD rats were randomly divided into control group, model group, treatment group and autonomous recovery group, ten rats in each. Animal model of dampness blocking spleen and stomach was established. Such indexes as content of D-lactic acid and activities of DAO, Na+-K+-ATPase and Ca2+-ATPase were tested. Result: Compared with the blank group, the content of D-lactic acid increased (P<0.01)and the activities of Na+-K+-ATPase and Ca2+-ATPase decreased obviously(P<0.01)except DAO (P>0.05). The general trend in Pingwei powder group treatment was better than that in natural recovery. Conclusion: Pingwei Powder can markedly recovery the damage of intestinal barrier and ion pump in rats with syndrom of dampness blocking spleen and stomach.关键词：Pingwei Powder;dampness blocking spleen and stomach syndrome;intestinal barrier
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发布时间：2024-01-04 - 摘要：ObJective: To investigate in vivo nasal mucosa absorption of baicalin and its phospholipid complex in rats; to compare the effect of the baicalin-phospholipid complex on cerebral edema and neurological impairment by sublingual iv administration and intranasal administration. Method: The nasal absorption in vivo in rats was used to compare the intranasal absorption of baicalin and its phospholipid complex.The model of focal ischemic reperfusion inJury of middle cerebral artery was built in rats by placing an intraluminal suture and the prevention and treatment of baicalin phospholipid complex was observed after sublingual iv administration and intranasal administration. Result: The nasal mucosa absorption rate of baicalin-phospholipid complex in rat is significantly greater than that of baicalin, they are (12.2±2.04)×10-3 ·min-1 and(9.60±1.34)×10-3 ·min-1 accordingly. The neurologic function scores are 1.66±0.68 and 1.78±0.78 respectively. They manifest that prevention and treatment effect of baicalin-phospholipid complex by intranasal administration was better than by that of sublingual iv administration against cerebral edema and neurological impairment caused by cerebral ischemic inJury in rats. Moreover, the fresh content of brain tissue is (79.10±0.65)% and (79.48±0.76)% separately. Conclusion: The in vivo absorption and efficacy of baicalin-phospholipid complex was better than that of baicalin, and nasal intranasal administration is superior to sublingual iv administration.关键词：baicalin;phospholipid complex;intranasal administration;brain ischemia-reperfusion
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发布时间：2024-01-04 - 摘要：ObJective: To study the inhibitory effect of Compound Liuyueqing(CLYQ) on the synthesis of Collagen type I (Col I) in hepatic tissues in rats with hepatic fibrosis. Method: Hepatic fibrosis model of SD rats was induced by subcutaneous inJection (sc) of CCl4. Hepatic fibrosis SD rats were divided into 5 groups randomly: model control group, colchicina-group, high dose, middle dose and low dose of CLYQ groups, and normal SD rats were the blank contol group. Model control group and blank contol group were treated with normal saline, and the other groups were treated with corresponding drugs for four consecutive weeks by intragastric administration (ig). The relative quantification of Col I mRNA expression in hepatic tissues was detected by real time fluorescence quantitative PCR, and the quantification of Col I protein expression was detected by immunohistochemistry. Result: Compared with the model group, the various dosage groups of CLYQ could down regulate significantly Col I mRNA expression (P<0.01) and showed a dose-dependent relationship according to the results of real time fluorescence quantitative PCR. The results of immunohistochemistry showed that after treamented with CLYQ the area and the intensity of Col I protein expression in hepatic tissues were reduced and weakened significantly (P<0.05, or P<0.01) compared with that of the model group. Col I protein expression was reduced obviously in a dose=dependent manner. Conclusion: CLYQ can down-regulate the expression of Col I mRNA and protein synthesis in hepatic tissues of hepatic fibrosis rats. The results suggest that CLYQ can reduce the collagen, thus suppressing the deposition of ECM. Hence CLYQ has the marked effect of anti-hepatic fibrosis.关键词：Compound Liuyueqing;hepatic fibrosis;collagen type I;real time fluorescence quantitative PCR;immunohistochemistry
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发布时间：2024-01-04 - 摘要：ObJective: To study the preventive effects of the combination of ginseng and saffron for acute mountain sickness. Method: Kunming mice were randomized into 5 groups (control, Nuodikang 0.56 g ·kg-1, the combination of ginseng and saffron 20, 10, 5 g ·kg-1). The combination of ginseng and saffron was administered to mice once daily for 7 days. One hour after the last medication,the survival time was observed in mice using normobaric hypoxia. The time of swimming and the death rate were investigated in mice using hypobaric hypoxia. Result: The combination of ginseng and saffron (20, 10, 5 g ·kg-1) prolonged the survival time in mice under normobaric hypoxia and the swimming time in mice under hypobaric hypoxia, but had no significant effect on the death rate in mice with hypobaric hypoxia. Conclusion: The combination of ginseng and saffron has anti-hypoxia capacity in mice and can be used to prevent acute mountain sickness induced by environmental hypoxia.关键词：acute mountain sickness;Ginseng;saffron;hypoxia
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发布时间：2024-01-04 - 摘要：ObJective: To explore effects of ganoderma lucidum polysaccharides on expression of hippocampal N-methyl-D-aspartate(NMDA) receptor 2B(NR2B) in aging rats induce by D-gal. Method: The permanent dosage (sc) of D-gal was applied to SD rats to establish the aging model. Different concentrations of Ganoderma lucidum polysaccharides were individually administrated (ig) for 10 days. The Morris water maze was applied to validate the learning in rats. With immunohistochemitry and Western Blotting, the expression of NR2B was detected on tissues of CA1 and CA3 regions in hippocampus in rats. Result: As compared with the model, rats treated with G. lucidum polysaccharides (125, 100 mg ·kg-1) had longer durations of crossover platform and the persentage of swimming time in Ⅳ quadrant, as well as the increased expression of NR2B in hippocampus (P<0.01, P<0.01). Conclusion: G. lucidum polysaccharides would play important roles in promoting learning ability for aging rats induced by D-gal. The up-regulation of NR2B expression in hippocampus could be contributed to one of the related mechanisms.关键词：Ganoderma lucidum polysaccharides;experimental aging;hippocampus;N-methyl-D-aspartate receptor 2B
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发布时间：2024-01-04 - 摘要：ObJective: To study the effects of removing blood stasis and anti-inflammatory of Dieda Xiaotong Tablet to provide an experimental foundation for clinical application. Method: The removing blood stasis effect was demonstrated with rat and guinea pig models of hemostasis induced by local punching and clamping accordingly. The anti-inflammatory effect was determined with the models of the swelling ear in mice and cotton ball induced granuloma in rat. The treatment (ig) of Dieda Xiaotong Tablet lasted for 7 days. Result :Dieda Xiaotong Tablet markedly reduced blue stain area of skin and Evans content of in hemostasis rats. It could also suppress ear swelling caused by dimethylbenzene in mice and cotton ball induced granuloma in rat (P<0.01). Conclusion: Dieda Xiaotong Tablet possesses obvious effect of removing blood stasis and anti-inflammatoion.关键词：Dieda Xiaotong Tablet;pharmacological experiment;removing blood stasis;anti-inflammation
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发布时间：2024-01-04 - 摘要：ObJective: To observe influence of Yiqi Fuyuan Tang(YQFYT) combined with chemotherapy on apoptosis of colorectal cancer Lovo cells and the related molecular mechanism. Method: Experiment was divided into 5 groups: YQFYT-containing serum group, chemotherapeutic drugs-containing serum group, YQFYT+chemotherapeutic drugs-containing serum group, serum control group and blank control group. Apoptosis of colorectal cancer Lovo cells was investigated with AO/EB double fluorescent staining and single staining of Annexin V-FITC for each group. Expression of apoptosis proteins Bcl-2 and Bax of Lovo cell was determined. Result: The results indicated that YQFYT-containing serum had action of inducing apoptosis in colorectal cancer Lovo cells, and enhanced the effects of induced apoptosis of chemotherapeutic drugs. Western blot assay showed that:①Bcl-2, Bax protein expression was not significantly different between serum control group and blank control group; ②There was significant difference between treatment groups (P<0.01). The order from weak to strong of the Bcl-2 protein expression was as follows: YQFYT+ chemotherapeutic drugs-containing serum group, chemotherapeutic drugs-containing serum group, YQFYT-containing serum group, serum control group. The order for bax protein expression was Just the opposite. Conclusion: Serum YQFYT-containing serum has action of inducing apoptosis in colorectal cancer Lovo cells, and can enhance the effects of chemotherapeutic drugs for inducing apoptosis. Down-regulation of Bcl-2 protein expression and up-regulation of Bax protein expression might be one of the main mechanisms for apoptosis of Lovo colon cancer cells.关键词：Yiqi Fuyuan Tang;Chinese medicine-containing serum;apoptosis;Bcl-2, Bax expression
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发布时间：2024-01-04 - 摘要：ObJective: To observe the effects of Coix Seed Oil on analgesia and hemostasis in mice. Method: The effects of Coix Seed Oil on analgesia and hemostasis in mice were observed through the methods of body stretching, tail cutting and blood coagulation in glass capillary accordingly, and the influence of Coix seed Oil on the organ index of spleen and thymus in mice was also observed. Result: Compared with control group, 2.0 g ·kg-1 and 1.0 g ·kg-1 Coix Seed Oil had significant effects of prolonging the stretching incubation period, decreasing the stretching times and shortening the clotting time in mice, and could significantly increase the organ index of spleen and thymus in mice. Conclusion: Coix Seed Oil has the effects of analgesia and hemostasis, and can promote the growth of spleen and thymus in mice.关键词：Coix Seed Oil;mice;analgesia;hemostasis
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发布时间：2024-01-04 - 摘要：ObJective: To investigate the changes of endogenous metabolites concentrations in the urine of normal rats administered with Cinnamomi Cortex water extract. Method: The administered group were administered orally with Cinnamomi Cortex water extract for 30 days.Urine was collected at 0, 1, 8, 15, 22, 29 d after administration respectively and analyzed by LC/MS/MS.The acquired data were analyzed by PCA, OSC-PLS-DA. Result: After administration for 8 days, endogenous metabolites concentration in the urine of administered rats changed apparently. Through OSC-PLS-DA analysis, 21 biomarkers were found. The identified biochemical effects of these biomarkers were associated with the pharmacological actions of Cinnamomi Cortex, including increased concentrations of CPA (18 ∶1(11Z)/0 ∶0), gamma-linolenyl carnitine and decreased concentrations of deoxyuridine, CerP(d18 ∶1/22 ∶0). Conclusion: The administration of Cinnamomi Cortex water extract may change the cell signals and basic metabolism significantly in rat.关键词：Cinnamomi Cortex;metabonomics;biomarker
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发布时间：2024-01-04 - 摘要：ObJective: To investigate the effect of pretreatment by vagotomy and nicotine on systemic inflammatory reaction syndrome (SIRS) in a rat model of "two-hit", and to provide new ideas for the clinical treatment of SIRS. Method: Forty male SD rats were randomly divided into four groups, i.e. group A (control) without treatment, group B(unilateral vagotomy), group C (nicotine intervention), group D (unilateral vagotomy+nicotine intervention). The above interventions lasted 7 days. Afterwards, group B, group C and group D were treated by hemorrhagic shock and ip LPS for inducing SIPS. Twelve hours later, blood was with drawn to test the blood cell count, TNF-α,IL-6,LDH,CPK BUN, Cr, ALT, AST and TBA. Result: Vagotomy could promote inflammation, and nicotine pretreatment could inhibit inflammation. Joint pretreatment of vagotomy+nicotine could produce and inflammatory state in between. Conclusion: Nicotine in the secondary cholinergic anti-inflammatory pathways appears to be independent to the integrity of vagus nerve. It may function independently.关键词：two-hit;vagotomy;nicotine pretreatment;systemic inflammatory reaction syndrome
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发布时间：2024-01-04 - 摘要：ObJective: To evaluate the preventative and therapeutical effect of Xiaoer Feire Kechuan oral solution on the immunocompromised mice infected by influenza virus A (H1N1). Method: ICR mice were inJected cyclophosphamide to establish immunocompromised mice model, at the same day, the mice were infected by influenza A (H1N1) by nasal drops. The lung index, death rate, death protection rate and life prolongation were observed; The virus load in lungs was detected by real-time RT-PCR and the level of immune factors in blood serum were detected by ELISA. Result: With preventative and therapeutical treatment of Xiaoer Feire Kechuan oral solution, the lung index, death rate and the virus load in lung tissues of immunocompromised mice which were infected by influenza virus FM1 strain and PR8 strain decreased significantly, while the life time of immunocompromised mice was prolonged by the oral solution and CD4/CD8 ratio in serum increased significantly. Conclusion: Xiaoer Feire Kechuan oral solution could inhibit virus pneumonia in immunocompromised mice caused by influenza virus A(H1N1)significantly.关键词：Xiaoer Feire Kechuan oral solution;influenza virus;immunocompromised mice;viral pneumonia
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发布时间：2024-01-04 - 摘要：ObJective: Observing the protective effect of Zhenwu Decoction on renal interstitial fibrosis in rat, to explore its role of anti-renal fibrosis and Ⅰ,Ⅲ collagen fibers affected in renal fibrosis. Method :The rat model of chronic renal failure was used. Niaoduqing control group was treated by Niaoduqing solution, Zhenwu Decoction group was treated by Zhenwu Decoction concentrated solution, up to 28 days. After 4 weeks, blood samples were taken from the femoral artery. Serum creatinine, blood urea nitrogen, serum calcium and phosphorus were detected. Renal pathological staining for paraffin sections was carried out by hemotoxylin and eosin staining, periodic acid-silver methenamine (PASM) staining,Masson staining,Sirius red staining, Mallory staining. HE staining was randomly selected for semi-quantitative analysis in tubulointerstitial lesions. Periodic acid-silver methenamine staining,Masson staining,Sirius red staining, Mallory staining were randomly selected for kidney pathology analysis. Result :In PASM mesangial integral optical density quantitative model of mesangial proliferative,Masson staining fibrosis in the area of renal interstitial, Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be better than model group and Niaoduqing control group (P<0.05). Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be less than model group in renal interstitial of type Ⅰ and Ⅲ distribution area (P<0.05). Conclusion :Zhenwu Decoction shows effect of anti-renal interstitial fibrosis. And it inhibit Ⅰ, Ⅱ collagen fibers proliferation, which may be the mechanism for Zhenwu Decoction to treat renal interstitial fibrosis and chronic renal failure.关键词：Zhenwu Decoction;chronic renal failure;rat;renal interstitial fibrosis
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发布时间：2024-01-04 - 摘要：ObJective :To examine the effect of corn polysaccharide on cellular immunological and nonspecific immunological function in mice. Method: The doses of 0.026 7, 0.267, 0.8 g ·kg-1 were given to mice by ig for 30 days. Delayed tvpe hypersensitivity, carbon clearance test, lymphocyte transformation test induced by ConA and macrophage phagocytosis of chicken red blood cell test were conducted separately. Result :Compared with the normal control group, corn polysaccharide could promote DTH and ability of carbon clearance, but had no effect on macrophage phagocytosis and lymphocyte transformation. Conclusion :Corn polysaccharide can promote the function of cellular immunity of mice, and also can strengthen the ability of carbon clearance in mice.关键词：corn polysaccharide;cellularimmunity;carbon clearance
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发布时间：2024-01-04 - 摘要：ObJective: To observe the intervention effects and mechanism of Bushen Tongluo decoction on bone metabolism and density in the osteoporosis rats. Method :Rat models of the osteoporosis were established by bilateral ovariotomy. The rats were divided into six groups randomLy:the nomal group,model group,XinLinGuBao group,Bushen-based decoction group, Tongluo decoction group, Bushen Tongluo decoction group. After ten weeks treatment,the rats weight,the serum calcium,the serum phosphorus,the serum alkaline phosphatase and the bone density. Result :The weight of the osteoporosis rats increased, the serum calcium decreased, the serum phosphorus increased, the serum alkaline phosphatase increased and the bone density decreased in model group as compared with the normal group(P<0.05).The weight gain of the osteoporosis rats were inhibited in each treat group as compared with model group,but there were no significant difference. The serum calcium increased, the serum phosphorus decreased in each treat group as compared with model group,and there were significant difference in Bushen Tongluo decoction group.The serum alkaline phosphatase decreased obviously in each group as compared with model group(P<0.01).The bone density increased in each group as compared with model group,and there were significant difference in Bushen-based decoction group.(P<0.05) and in Tongluo decoction group and Bushen Tongluo decoction group(P<0.01). The numerus of Bushen Tongluo decoction group were higher than the numerus of Bushen-based decoction group or Tongluo decoction group. Conclusion :Bushen Tongluo decoction group can effectively decrease high dysbolism of bone metabolism,increase bone density in the osteoporosis rats. Bushen decoction and Tongluo decoction have good synergism.关键词：Bushen Tongluo decoction;osteoporosis;bone metabolism;bone density;empirical study
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发布时间：2024-01-04 - 摘要：ObJective: To compare the influence of different alcohol extraction on the acute toxicity of Sophorae Tonkinensis Radix et Rhizome , caused by different extracted crafts. Method : Separately using percolate and reflux extracted methods to prepare the extracts of different processed products. The classical acute toxicity methods were used in this study, the acute toxical symptoms, signs, death time and accumulated death rate were recorded and the LD50were calculated by Bliss method. Result : The LD50 and its 95% confident limit of reflux and percolation extracts on mice were respectively 27.135(24.869-29.622)g ·kg-1 and 13.399(12.016-14.899)g ·kg-1, which are equal to 316.6 and 156.3 times of the daily dosage in clinical, the main acute toxical symptoms such as: hyperactivity, restlessness, intermittent convulsion, breathlessness and died of respiratory failure eventually. The body weight between survival and normal mice had obvious difference (P<0.05), this difference disappeared after 7 d administration. Conclusion : The acute toxicity of different alcohol extraction of Sophorae Tonkinensis Radix et Rhizome is: percolation extracts>reflux extracts, explaining the key factors are temperature and concentration, especially the temperature, that affect the acute toxicity result expression of different alcohol extraction.关键词：Sophorae Tonkinensis Radix et Rhizome;alcohol extracted craft;acute toxicity
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发布时间：2024-01-04 - 摘要：ObJective: The effects of the different Salvianolic acid B (Sal B) and Paeonol (Pae) drug combinations on isolated vascular and acute myocardial ischemia-reperfusion inJury (MI/RI) were investigated in rats. Method: A increase-decrease design method of experiment with baseline geometric proportion was established to design Salvianolic acid B and Paeonol drug combinations. The changes in isometric tension were recorded by force transducers connected to the system. Vaso-relaxant effects of the drug combinations were observed on the contraction of rat thoracic aorta rings. Acute myocardial ischemia model was made by ligation of left anterior descending artery in the anesthetized open chest rats.Changes in baseline geometric was designed for the different combinations, the effects of different combinations on electrocardiogram(ECG), myocardial infarction square(MIS), malondialdehyde(MDA), superoxide dismutase(SOD)and nitrogen monoxidum(NO) were measured, and effects on isoenzyme of creatine-kinase(CK-MB) were also determined. Result : The drug combinations on vasorelaxant activity was superior to model group and can reduce the degree of myocardial ischemia(∑-ST) and the infarction square(MIS), Moreover, it also can reduce MDA and CK-MB in serum, and improve SOD and NO activity in MI/RI. The ratio of 3 ∶1 was the best in the drug combinations. Conclusion :The drug combinations of Sal B and Pae have some protective effects on acute myocardial ischemia-reperfusion inJury in rats. Some synergistic or additive effects may exist between Sal B and Pae.关键词：salvianolic acid B;paeonol;myocardial ischemia-reperfusion injury
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发布时间：2024-01-04 - 摘要：ObJective: To study the effect and mechanism of Qizhi Tongluo Capsule assisted with aspirin on experimental cerebral ischemia-reperfusion inJury of rats. Method: The middle-cerebral-artery-occlusion(MCAO) was used to induce focal cerebral ischemia-reperfusion inJury model of rats. The content of superoxide dismutase(SOD),malondialdehyde(MDA),nitrogen monoxide(NO),Ca2+-ATPase,Na+-K+-ATPase in brain tissue and NO,nerve growth factor (NGF),vascular endothelial growth factor (VEGF) in serum and hippocampus pathologic changes were observed to research the effect of Qizhi Tongluo Capsule assisted with aspirin. Result: Compared with using aspirin alone, the content of SOD in brain was increased and MDA in brain was reduced significantly by the combination use of Qizhi Tongluo Capsule and aspirin. Meanwhile, NO levels in brain and serum were reduced significantly(P<0.05 or P<0.01), Ca2+-ATPase,Na+-K+-ATPase in brain tissue and NGF,VEGF in serum were increased significantly(P<0.05 or P<0.01). Conclusion: It played a synergistic role in recovery that Qizhi Tongluo Capsule assisted with aspirin on cerebral ischemia-reperfusion model in rats, and it will be a beneficial assistance to reduce the damage of ischemic.关键词：Qizhi Tongluo Capsule;aspirin;adjuvant drug;cerebral ischemia-reperfusion;rat
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发布时间：2024-01-04 - 摘要：ObJective: To investigate possible mechanisms of the beneficial effects of Radix et Rhizoma Rhei prepared with wine on the contents and expressions of plasminogen activator inhibitor-1(PAI-1) in atherosclerotic (AS) rabbits induced by high lipid diet. Method: Thirty-two healthy male New-Zealand rabbits were randomly divided into four groups(n=8 each): Normal control group(N), AS model group(M), low dose(L)and high dose(H)Radix et Rhizoma Rhei prepared with wine groups. After 12-weeks treatment,the protein of PAI-1 was observed by immunohistochemistry method, and PAI-1 mRNA in aortic tissue were detected by RT-PCR method. Result: In low dose group, the expression of PAI-1 mRNA and protein in aortic tissue decreased significantly compared with AS model group(P<0.05 or P<0.01). In high dose group, the expression of PAI-1 mRNA and protein in aortic tissue were significantly increased compared with the other three groups.(P<0.05 or P<0.01). Conclusion: Radix et Rhizoma Rhei prepared with wine may play a dual role in controlling of PAI-1 gene expression.关键词：Radix et Rhizoma Rhei prepared with wine;atherosclerosis;plasminogen activator inhibitor type-1
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发布时间：2024-01-04 - 摘要：ObJective: To investigate the correlation between tumor necrosis factor-α (TNF-α) and t uveitis. Method :The polymerase chain reaction was employed to detect 60 cases of uveitis, peripheral blood TNF-α level and gene polymorphism were also determined. Result :TNF-α in peripheral blood was significantly higher (P<0.05) in observation group. After PCR amplification, TNF-α gene fragment of the size of 117b was found; The control group no specific amplification products. PCR/RFLP analysis showed that Group 2 TNF-α gene had three kinds of restriction patterns, i.e. TNFα-308A/A homozygous without digestion, TNFct-308G/G homozygotes with completed digestion and TNFα-308G/A heterozygote produces with partial digestion. Had genotype frequencies from the high to the generation were in the order of G/G type,G/A-type, A/A type. But the two groups had significant difference (P<0.05). Conclusion : TNF-α and the incidence of uveitis are correlated.关键词：uveitis;TNF-α;correlation
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发布时间：2024-01-04 - 摘要：ObJective: To explore the syndrome distribution of vascular dementia based on its prescription analysis so as to direct the clinical and research work even better. Method : Through the analysed prescriptions which were from the 152 pieces of literature(seized from VIP database in 2000-2008) on the treatment of vascular dementia by Traditional Chinese Medicine, explore the vascular dementia's syndrome distribution. Result : The invigorating the circulation of blood,supplement of Yin and Yang,nourishing Qi,enrich the blood,restoring a clear head,expectorant drugs and calming liver wind are much more used. The dominated syndrome of vascular dementia are blood stasis, deficiency of kidney yin and yang. Conclusion : The prescription analysis result embodies the characteristics and laws of modern Chinese medicine's treatment on vascular dementia, which also reveal this disease's syndrome distribution rules, ultimately could better guide clinical and research work.关键词：vascular dementia;prescription analysis;syndrome distribution
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发布时间：2024-01-04 - 摘要：ObJective: To observe the effect of a acupuncture medication combined regimen(AMCR) on the vascular endothelial function and the velocity of blood flow in the vertebral-basilar artery in patients with vertebro-basilar insufficiency(VBI). Method : Adopting randomized and controlled principle, a trial was conducted on 98 patients with VBI by randomized them into two groups, they were administered AMCR (Group A) and flunarizine hydrochloride capsule (Group B)respectively, for 20 days. Changes of the velocity of blood flow in the vertebral-basilar artery, as well as the levels of serum nitric oxide(NO), plasma endothelin-1(ET-1),6-keto-prostaglandin1α(6-keto-PGF1α) and thromboxane B2(TXB2) were observed before and after treatment. Result :After treatment the velocity of blood flow in the vertebral-basilar artery increased and clinical symptoms improved, with serum NO and 6-keto-PGF1α levels elevated, plasma ET-1 and TXB2 decreased in all the two groups(P<0.05).But the inter-group comparisons showed that the effect in Group A was superior to the other group in increasing the velocity of blood flow in the vertebral-basilar artery,elevating serum NO and 6-keto-PGF1α levels and decreasing plasma TXB2(P<0.05). Conclusion : The AMCR has markedly effect in increasing the velocity of blood flow in the vertebral-basilar artery, it could obviously improve the symptoms and vascular endothelial function, and inhibit the level of inflammatory factor in patients with VBI.关键词：vertebro-basilar insufficiency;Huatan Dingxuantang;acupuncture medication combined;vascular endothelial function
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发布时间：2024-01-04 - 摘要：People have done various researches on plants that belong to the Vitex L., especially V. negundo and V. trifolia. These researches had discovered different types of chemical compositions which include flavonoids, terpenes, lignans, sterols, anthracenes, volatile oil, etc. Experimental results confirmed that flavonoids in Vitex L. can be used in anti-aging, prevention and treatment of cardiovascular diseases. These results showed preferable value on exploitation and utilization of Vitex L. Since Vitex L. is widely distributed with high diversity in our country, some of these plants have been used in folk medicine. If we can further exploit the Vitex L. with scientific connotation, a huge consumer market will be ahead of us. Simultaneously, it will become a kind of economic crops which involve people's livelihood and highly related to regional development.关键词：Vitex L.;Vitex negundo;Vitex trifolia
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发布时间：2024-01-04 - 摘要：Iridoids, which exhibit various biological activities, are a kind of important secondary metabolites in plants. Researches on distribution and biological activities of iridoids were reviewed. Iridoids have a wide range of biological activities, such as diabetic treatment, protecting liver, anti-inflammatory, antibacterial, antitumor activity et al. All these could serve as referential basis for the effective exploration of medicinal plants and further medical using of iridoids.关键词：iridoids;distribution;biological activity
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发布时间：2024-01-04 - 摘要：This article summarizes the relative research and application of ginseng and saffron on preventing acute mountain sickness (AMS). In the guidance of prescription principles of monarch,minister,assistant and guide theory, the author analyses and discusses the possibility of compatible application of ginseng and saffron on preventing AMS. It is suggested that high dose ginseng combined with low dose saffron can be used to prevent AMS. The article provides a reference for further studies on new drugs and functional foods for preventing AMS.关键词：acute mountain sickness;Ginseng;saffron;prescription compatibility
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发布时间：2024-01-04 - 摘要：Total glucosides of paeon, which belongs to Ranunculaceae plants and contains a variety of ingredients, has many pharmacological effects. It affect cell immunity, humoral immunity and inflammation process in autoimmune diseases in many aspects and have reliable curative effects on such autoimmune diseases as rheumatoid arthritis and systemic lupus erythematosus.关键词：total glucosides of paeon;immunity;autoimmune disease
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发布时间：2024-01-04 - 摘要：The effects of chemical synthetic drugs for treatment of depression are obvious, but long-term use will bring with serious side effects. Chinese medicine is mild and has few side effects, prescriptions containing bupleurum as the monarch are widely used in treatment of depression. In this paper, experimental and clinical studies about this categrey of prescriptions were reviewed for treatment of depression. It demonstrates that prescriptions containing bupleurum as the monarch play a significant role in treatment of depression.关键词：depression;stagnation of liver;Bupleurum chinense;prescriptions containing bupleurum as the monarch
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发布时间：2024-01-04 - 摘要：This is a summary regarding the process of ointment's preparation and quality control, including herbal extraction,preparation,test of cutaneous stimulation,determination method of active ingredient,drug release, penetration,absorption etc. The specialty and advantage are discussed when Chinese ointment is used in cosmetic industry.The prospect of Chinese ointment is promissing cosmetics field.关键词：Chinese materia medica;ointment;cosmetics
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发布时间：2024-01-04 - 摘要：Taking Cough Theory in Yi Guan written by Zhao Xian-ke in Ming Dynasty as the key point and based on his academic thought on the pathogenesis of cough and clinical diagnosis and treatment, this thesis analyzes Zhao Xian-ke's unique five elements theory and Ming Men theory in the treatment of cough as the reference in related field.关键词：Yi Guan;Zhao Xian-ke;cough;five elements;Mingmen
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发布时间：2024-01-04
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