最新刊期
卷 16 ， 期 18 ， 2010
- 摘要：Objective:To compare the powder properties between common powder and micropowders of Angelica to provide experimental evidence for the superfine grinding application.Method:Morphology structure,hygroscopic property,liquidity,surface area and porosity of Angelica powders were investigated;the contents and the dissolution of ferulic acid of Angelica powders were determined by HPLC.Result: With the decreasing of particle size of powders,the surface area,pore volume and moisture increased,and the liquidity decreased;the contents of ferulic acid from four Angelica powders were in the order of: common powder > ultrafine powder I > ultrafine powder II > ultrafine powder III;but with the crushing level increased,dissolution rate and the total dissolution of the ultrafine powder II was higher than levels of particle size in other angelica powder.Conclusion: An appropriate degree of micronization is helpful for the dissolution of the active components in Angelica.关键词：Angelica;superfine grinding;power properties;dissolution
- 0
- |
- 1085
- |
- 63
发布时间：2024-09-23 - 摘要：Objective: To compare the powder properties between common powder and micropowders of Angelica to provide experimental evidence for the superfine grinding application. Method: Morphology structure, hygroscopic property, liquidity, surface area and porosity of Angelica powders were investigated; the contents and the dissolution of ferulic acid of Angelica powders were determined by HPLC. Result : With the decreasing of particle size of powders, the surface area, pore volume and moisture increased, and the liquidity decreased; the contents of ferulic acid from four Angelica powders were in the order of: common powder > ultrafine powder I > ultrafine powder II > ultrafine powder III; but with the crushing level increased, dissolution rate and the total dissolution of the ultrafine powder II was higher than levels of particle size in other angelica powder. Conclusion : An appropriate degree of micronization is helpful for the dissolution of the active components in Angelica.关键词：Angelica;superfine grinding;power properties;dissolution
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: Total flavones sustained-release tablets of Dracocephalum were prepared and the influencing factors on release of the tilianin of Dracocephalum were studied . Method: The Dracocephalum total flavones sustained-release tablets were compressed after wet granulating the extracts of Dracocephalum total flavones with HPMC. Then, the influencing factors on release of tilianin were investigated by determining release-rate of tilianin at different time to draw release curve and calculate K value (a kind of index comprehended with cumulative release at 2, 6, 12 h, respectively) and f2 (similar factorof release curve). Result : The main influencing factors on release-rate of tilianin were the type of HPMC and the ratio of starch to lactose; the amount of HPMC and adhesive were the secondary. Conclusion : The release of tilianin of Saussurea involucrate could be controlled effectively by selecting HPMC K15M as matrices.关键词：Dracocephalum total flavones;sustained-release tablets;HPMC;in vitro dissolution
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:Total flavones sustainedrelease tablets of Dracocephalum were prepared and the influencing factors on release of the tilianin of Dracocephalum were studied.Method:The Dracocephalum total flavones sustainedrelease tablets were compressed after wet granulating the extracts of Dracocephalum total flavones with HPMC.Then,the influencing factors on release of tilianin were investigated by determining releaserate of tilianin at different time to draw release curve and calculate K value（a kind of index comprehended with cumulative release at 2,6,12 h,respectively） and f2（similar factorof release curve）.Result: The main influencing factors on releaserate of tilianin were the type of HPMC and the ratio of starch to lactose;the amount of HPMC and adhesive were the secondary.Conclusion: The release of tilianin of Saussurea involucrate could be controlled effectively by selecting HPMC K15M as matrices.关键词：Dracocephalum total flavones;sustained-release tablets;HPMC;in vitro dissolution
- 0
- |
- 238
- |
- 11
发布时间：2024-09-23 - 摘要：Objective:To compare the dissolution of Tianshu dropping pill（A）,capsule（B） and tablet（C） in vitro.Method:Small vessel and HPLC method were applied to determine the accumulated dissolution rate of ferulic acid at the different time,data were analyzed by Weibull distribution,and the dissolution rate was evaluated by similar factor method.Result: The dissolution results of 3 kinds of Tianshu were all up to the standards of Chinese Pharmacopeia;the Td of Tianshu（A,B and C） preparations were as follows: 6.15,11.30 and 18.66 minute;the similarity factors（f2） of preparation B and C were less than 50,and the dissolutions of preparation B and C were significantly different from preparation A.Conclusion: Among them,the dissolution profile of preparation A was superior to other preparations.This provides the basis of improving the preparation process,evaluating and controlling the quality.关键词：Tianshu preparation;dissolution in vitro;HPLC;Weibull distribution;similarity factor
- 0
- |
- 188
- |
- 8
发布时间：2024-09-23 - 摘要：Objective:To investigate the preparation of the novel liposome composed of hydrogenated soybean phosphatidylcholine（HSPC） and soybean phosphatidylcholine（SPC） containing zedoary turmeric oil（ZTO）.Method:HPLC analysis and spectrophotometry analysis were used to determine the contents of curcumol and ZTO in ZTO liposomes both composed of HSPC and SPC respectively.The ZTO novel liposomes were prepared by ethanol injection method.The best prescription of ZTO liposome both composed of HSPC and SPC was determined through orthogonal design,using the drug content of liposomes as the evaluation index.Result: When ZTO novel liposome was prepare with the best prescription,both the entrapment efficiency stability and the drug content were high.Conclusion: The method was accurate and applicable.The preparation was feasible and the quality of the novel liposome was stable and controllable.关键词：zedoary turmeric oil;liposomes;preparation;HSPC;SPC
- 0
- |
- 387
- |
- 10
发布时间：2024-09-23 - 摘要：Objective: To investigate the preparation of the novel liposome composed of hydrogenated soybean phosphatidylcholine (HSPC) and soybean phosphatidylcholine (SPC) containing zedoary turmeric oil (ZTO). Method: HPLC analysis and spectrophotometry analysis were used to determine the contents of curcumol and ZTO in ZTO liposomes both composed of HSPC and SPC respectively. The ZTO novel liposomes were prepared by ethanol injection method. The best prescription of ZTO liposome both composed of HSPC and SPC was determined through orthogonal design, using the drug content of liposomes as the evaluation index. Result : When ZTO novel liposome was prepare with the best prescription, both the entrapment efficiency stability and the drug content were high. Conclusion : The method was accurate and applicable. The preparation was feasible and the quality of the novel liposome was stable and controllable.关键词：zedoary turmeric oil;liposomes;preparation;HSPC;SPC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To study the percutaneous absorption of ginsenoside Rg1 in Shangke spray in vitro. Method: The transdermal permeation tests of ginsenoside Rg1 in Shangke Spray were performed through excised rat skin in Franz diffusion cells. Ginsenoside Rg1 concentration was determined by HPLC. Result : Ginsenoside Rg1 could penetrate through rat skin. The permeation rate and the lag time were 0.161 mg ·cm-2 ·h-1 and 0.368 h respectively. Conclusion : The research provides experimental data for the design of shangke Spray.关键词：Shangke spray;Ginsenoside Rg1;percutaneous absorption
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the percutaneous absorption of ginsenoside Rg1 in Shangke spray in vitro.Method:The transdermal permeation tests of ginsenoside Rg1 in Shangke Spray were performed through excised rat skin in Franz diffusion cells.Ginsenoside Rg1 concentration was determined by HPLC.Result: Ginsenoside Rg1 could penetrate through rat skin.The permeation rate and the lag time were 0.161 mg.cm-2.h-1 and 0.368 h respectively.Conclusion: The research provides experimental data for the design of shangke Spray.关键词：Shangke spray;Ginsenoside Rg1;percutaneous absorption
- 0
- |
- 156
- |
- 5
发布时间：2024-09-23 - 摘要：Objective:To optimize technique of bran-processed Fructus Aurantii Immaturus.Method:Orthogonal design L9（3）4 was used to select the best processing technical parameters by rate of the paste,synephrine,naringin and hesperidin in the processing products.Result: The optimal procedure was suggested as follows: Diameter of Fructus Aurantii Immaturus was 1.5-2.5 centimeters and onetenth amount of bran by Fructus Aurantii Im-maturus weight was added,frying for one minutes at 180 degrees Celsius.Conclusion: The process is feasible.关键词：Fructus Aurantii Immaturus;orthogonal test;synephrine;naringin;hesperidin;processing technology
- 0
- |
- 373
- |
- 3
发布时间：2024-09-23 - 摘要：Objective: To optimize technique of bran-processed Fructus Aurantii Immaturus. Method: Orthogonal design L9(3)4 was used to select the best processing technical parameters by rate of the paste, synephrine, naringin and hesperidin in the processing products. Result : The optimal procedure was suggested as follows: Diameter of Fructus Aurantii Immaturus was 1.5-2.5 centimeters and one-tenth amount of bran by Fructus Aurantii Immaturus weight was added, frying for one minutes at 180 degrees Celsius. Conclusion : The process is feasible.关键词：Fructus Aurantii Immaturus;orthogonal test;synephrine;naringin;hesperidin;processing technology
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:Purify the total flavonoids from Hedyotis diffusa.with macroporous resin and establish optimum process for separating and purifying total flavonoids from H.diffusa.Method:The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests,and the separation tech-niques were evaluated by measuring the concentration of total flavonoids from H.diffusa with ultraviolet spectropho-tometer.Result: The HPD-826 macroporous resin gave the best effect of separation with the sampling rate 2 BV.h-1,concentration 1.0 g.L-1,pH 5 respectively,and the concentration of elution was 80% alcohol,eluting veloc-ity 2 BV.h-1,with pH 7.Conclusion: HPD-826 resin could be applied to purify total flavonoids from H.diffusa.关键词：total flavonoids;Hedyotis diffusa;macroporous resins;purification
- 0
- |
- 584
- |
- 21
发布时间：2024-09-23 - 摘要：Objective: Purify the total flavonoids from Hedyotis diffusa. with macroporous resin and establish optimum process for separating and purifying total flavonoids from H.diffusa. Method: The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests, and the separation techniques were evaluated by measuring the concentration of total flavonoids from H.diffusa with ultraviolet spectrophotometer. Result : The HPD-826 macroporous resin gave the best effect of separation with the sampling rate 2 BV ·h-1, concentration 1.0 g ·L-1, pH 5 respectively, and the concentration of elution was 80％ alcohol, eluting velocity 2 BV ·h-1, with pH 7. Conclusion : HPD-826 resin could be applied to purify total flavonoids from H.diffusa.关键词：total flavonoids;Hedyotis diffusa;macroporous resins;purification
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To optimize extraction technology of the polysaccharides from Chrysanthemum indicum. Method: Based on the single factors test, factors such as material to solvent ratio, extraction temperature and extraction time were optimized by orthogonal test L9(34). Result : The optimal technology conditions were as follows: extraction temperature was set at 90 ℃ and extraction time was 4 h with ratio of 1 ∶20. Conclusion : Under these conditions the polysaccharides yield of C.indicum was up to 3.32%.关键词：Chrysanthemum indicum;polysaccharides;extraction;orthogonal experimental design
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of the polysaccharides from Chrysanthemum indi-cum.Method:Based on the single factors test,factors such as material to solvent ratio,extraction temperature and extraction time were optimized by orthogonal test L9（34）.Result: The optimal technology conditions were as fol-lows: extraction temperature was set at 90 ℃ and extraction time was 4 h with ratio of 1∶ 20.Conclusion: Under these conditions the polysaccharides yield of C.indicum was up to 3.32%.关键词：Chrysanthemum indicum;polysaccharides;extraction;orthogonal experimental design
- 0
- |
- 510
- |
- 17
发布时间：2024-09-23 - 摘要：Objective: To determine the optimal processing method for Fructus Forsythiae. Method: HPLC was applied on the determination with Alltima C18 column (4.6 mm×250 mm, 5 μm) and methanol - water as mobile phase by a gradient elution program at a flow rate of 0.8 mL ·min-1. The detection wavelength was at 280 nm and column temperature was at room temperature. Result: The regression equation of Forsythiaside was Y =959 631.703 1 X +250 467.648 4, r =0.999 4,Linear range was 1.5-15 μg, The regression equation of phillyrin was Y =883 203.205 8 X -9 047.738 3, r =0.999 4,Linear range was 0.145-1.45 μg. The average recovery of Forsythiaside and Phillyrin was 97. 9% with RSD 2. 30% and 99. 2% with RSD 2. 27% (n= 6 ) respectively. Content of the Forsythiaside and Phillyrin was in the maximum yield with the processing methods of steaming for 20-30 min steaming. Conclusion: The method was accurate and repeatable, can be applied to the quality control of Fructus Forsythiae. The optimal processing method of Fructus Forsythiae was steaming for 20-30 min.关键词：Fructus Forsythiae;processing methods;forsythiaside;phillyrin
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To determine the optimal processing method for Fructus Forsythiae.Method: HPLC was applied on the determination with Alltima C18 column（4.6 mm × 250 mm,5 μm） and methanol-water as mo-bile phase by a gradient elution program at a flow rate of 0.8 mL.min-1.The detection wavelength was at 280 nm and column temperature was at room temperature.Result: The regression equation of Forsythiaside wasY = 959 631.703 1X + 250 467.648 4,r = 0.999 4,Linear range was 1.5-15 μg,The regression equation of phillyrin was Y = 883 203.205 8X-9 047.738 3,r = 0.999 4,Linear range was 0.145-1.45 μg.The average recovery of Forsythiaside and Phillyrin was 97.9% with RSD 2.30% and 99.2% with RSD 2.27%（n = 6） respectively.Content of the Forsythiaside and Phillyrin was in the maximum yield with the processing methods of steaming for 20-30 min steaming.Conclusion:The method was accurate and repeatable,can be applied to the quality control of Fructus Forsythiae.The optimal processing method of Fructus Forsythiae was steaming for 20-30 min.关键词：Fructus Forsythiae;processing methods;forsythiaside;phillyrin
- 0
- |
- 210
- |
- 12
发布时间：2024-09-23 - 摘要：Objective:To optimize the best β-cyclodextrin including technology conditions of volatile oil from Pericarpium Citri Reticulatae Viride by orthogonal design.Method:The saturated aqueous solution-ultrasound method was used.Result:The optimum preparation conditions were established as follows:the proportion of volatile oil to β-cyclodextrin was 1∶ 8（mL.g-1）,The concentration of alcohol was 90%,The ultrasonic time was 30 min,The water temperature was at 40 ℃ ～ 45 ℃.Conclusion:The different usage of β-cyclodextrin is the principal factor that influence the inclusion effect of volatile oil from Pericarpium Citri Reticulatae Viride,the method of this technology is simple,and the technological process is short,it is suitable for commercial process.关键词：Pericarpium Citri Reticulatae Viride;volatile oil;β-cyclodextrin;including compound;orthogo-nal design;GC-MS
- 0
- |
- 211
- |
- 14
发布时间：2024-09-23 - 摘要：Objective: To optimize the best β-cyclodextrin including technology conditions of volatile oil from Pericarpium Citri Reticulatae Viride by orthogonal design. Method: The saturated aqueous solution-ultrasound method was used. Result ：The optimum preparation conditions were established as follows：the proportion of volatile oil to β-cyclodextrin was 1∶8(mL ·g-1），The concentration of alcohol was 90%，The ultrasonic time was 30 min，The water temperature was at 40 ℃～45 ℃. Conclusion ：The different usage of β-cyclodextrin is the principal factor that influence the inclusion effect of volatile oil from Pericarpium Citri Reticulatae Viride，the method of this technology is simple，and the technological process is short，it is suitable for commercial process.关键词：Pericarpium Citri Reticulatae Viride;volatile oil;β-cyclodextrin;including compound;orthogonal design;GC-MS
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:通过对六味地黄汤的煮散和自动煎药机的煎出率进行比较研究,探讨不同煎煮方式的中药煎出率差异。方法:比较两种不同煎煮方式的煎膏率及用高效液相测定的马钱苷煎出率。结果:六味地黄汤煮散的煎膏率及马钱苷的煎出率都高于自动煎药机的煎出率。结论:应用中药煮散可以达到节约药材、提高中药的使用率的目的,有利于中药的可持续发展。关键词：马钱苷含量;总浸出物;煮散;自动煎药机
- 0
- |
- 462
- |
- 18
发布时间：2024-09-23 - 摘要：目的:对复方头孢克洛分散片进行最佳制剂处方优选。方法:以均匀分散时间为指标,采用正交设计试验,对复方头孢克洛分散片处方进行筛选。结果:最佳处方组成为头孢克洛62%,盐酸溴已新2%,预胶化淀粉20%,交联聚乙烯吡咯烷酮（PVPP）12%,硬脂酸镁2%,甜菊糖2%,均匀分散时间为40 s。结论:所选处方合理,分散均匀性好。关键词：复方头孢克洛分散片;正交试验;制备工艺
- 0
- |
- 152
- |
- 1
发布时间：2024-09-23 - 摘要：Objective:To analyse the difference of content of total lignanoid,wuweizi ester A and deoxy-schizandrin between Schisandra chinensis（Turcz.） Baill.and Schisandra sphenanthera Rehd.et Wils.Method:① Chromatometry with chromotropic acid and sulphuric acid as chromogenic agents was used to determine the total lig-nanoid;② HPLC was selected to determine the content of wuweizi ester A and deoxyschizandrin.Result: ① The content of total lignan in Schisandra chinensis was between 4.91%-93.60% and was twice higher than that in S.sphenanthera.② Schisantherin A and deoxyschizandrin were linear in the range of 0.20-1.00 μg and 0.387-1.94 μg respectively.The average recovery was 97.1% and 101.3% respectively.The content of schisantherin A and deoxyschizandrin in S.chinensis was lower than that in S.sphenanthera.Conclusion: There were differences on content of total lignanoid,wuweizi ester A and deoxyschizandrin between S.chinensis and S.sphenanthera.The HPLC method used for determination of wuweizi ester A and deoxyschizandrin was accurate and with good repeat-ability,and can be used to control the quality of Fructus Schisandrae.关键词：Fructus Schisandrae;lignanoid;wuweizi ester A;deoxyschizandrin;content determination
- 0
- |
- 353
- |
- 15
发布时间：2024-09-23 - 摘要：Objective: To analyse the difference of content of total lignanoid, wuweizi ester A and deoxyschizandrin between Schisandra chinensis(Turcz.) Baill. and Schisandra sphenanthera Rehd. et Wils. Method: ① Chromatometry with chromotropic acid and sulphuric acid as chromogenic agents was used to determine the total lignanoid; ② HPLC was selected to determine the content of wuweizi ester A and deoxyschizandrin. Result : ① The content of total lignan in Schisandra chinensis was between 4.91%-93.60% and was twice higher than that in S.sphenanthera. ② Schisantherin A and deoxyschizandrin were linear in the range of 0.20-1.00 μg and 0.387-1.94 μg respectively. The average recovery was 97.1% and 101.3% respectively. The content of schisantherin A and deoxyschizandrin in S.chinensis was lower than that in S.sphenanthera. Conclusion : There were differences on content of total lignanoid, wuweizi ester A and deoxyschizandrin between S.chinensis and S.sphenanthera. The HPLC method used for determination of wuweizi ester A and deoxyschizandrin was accurate and with good repeatability, and can be used to control the quality of Fructus Schisandrae.关键词：Fructus Schisandrae;lignanoid;wuweizi ester A;deoxyschizandrin;content determination
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: The solid phase extraction and capillary gas chromatography were introduced for determining 12 organoehiorine and pyrethroid pesticide residues in polygonum orientale. Method: The organochlorine and pyrethroid pesticides were extracted with n-hexane and acetone by ultrasonic method and cleaned up by Florisil solid-phase extraction (SPE) column. Then, the extract was separated by capillary column HP-5(0.32 mm×30 m,0.25 μm) and detected by (electron capture detector,ECD). The internal standards were used for quantitative determination. Result : A mixed standard solution prepared from primary standards of 9 organochlorine and 3 pyrethroid pesticide. The calibration curve for each of 12 organochlorine and pyrethroid pesticide was prepared covering the concentration ranges of 1 to 200 or 5 to 1 000 μg ·L-1. The average recoveries and RSD were in the range of 82.0%-106.8% and 0.2%-7.9％ at three organochlorine and pyrethroid pesticides levels respectively. The limits of detection were 0.15-13 μg ·kg-1. There were no organochlorine and pyrethroid pesticide detected in 15 batches samples. Conclusion : This method was effective, fast and accurate. It has good repeatability and suitable for the analysis of organochlorine and pyrethroid pesticide in polygonum orientale.关键词：polygonum orientale;organochlorine and pyrethroid;pesticide residues
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:The solid phase extraction and capillary gas chromatography were introduced for de-termining 12 organoehiorine and pyrethroid pesticide residues in polygonum orientale.Method:The organochlorine and pyrethroid pesticides were extracted with n-hexane and acetone by ultrasonic method and cleaned up by Florisil solid-phase extraction（SPE） column.Then,the extract was separated by capillary column HP-5（0.32 mm × 30 m,0.25 μm） and detected by（electron capture detector,ECD）.The internal standards were used for quantitative determination.Result: A mixed standard solution prepared from primary standards of 9 organochlorine and 3 pyre-throid pesticide.The calibration curve for each of 12 organochlorine and pyrethroid pesticide was prepared covering the concentration ranges of 1 to 200 or 5 to 1 000 μg.L-1.The average recoveries and RSD were in the range of 82.0%-106.8% and 0.2%-7.9% at three organochlorine and pyrethroid pesticides levels respectively.The limits of detection were 0.15-13 μg.kg-1.There were no organochlorine and pyrethroid pesticide detected in 15 batches samples.Conclusion: This method was effective,fast and accurate.It has good repeatability and suitable for the analysis of organochlorine and pyrethroid pesticide in polygonum orientale.关键词：polygonum orientale;organochlorine and pyrethroid;pesticide residues
- 0
- |
- 119
- |
- 6
发布时间：2024-09-23 - 摘要：Objective: To develop an HPLC method for determination of nine active components in complex prescription of salvia miltiorrhiza Bunge by wavelength switching. Method: This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0.1% phosphoric acid solution, the flow rate was 1.0 mL ·min-1,detecting wavelength was at 281,203 and 270 nm, and column temperature was 30 ℃. Result : Nine components were linear(r>0.999 0) in the range of the test concentration, and the recoveries of the method were between 94.87%-104.31%, respectively. Conclusion : This method is suitable for the quality control of this commonly used complex prescription.关键词：HPLC;complex prescription of salvia miltiorrhiza bunge;multiple active components;wavelength switching
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To develop an HPLC method for determination of nine active components in complex prescription of salvia miltiorrhiza Bunge by wavelength switching.Method:This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0.1% phosphoric acid solution,the flow rate was 1.0 mL.min-1,detecting wavelength was at 281,203 and 270 nm,and column temperature was 30 ℃.Result: Nine components were linear（r > 0.999 0） in the range of the test concentration,and the recoveries of the method were between 94.87%-104.31%,respectively.Conclusion: This method is suitable for the quality control of this commonly used complex prescription.关键词：HPLC;complex prescription of salvia miltiorrhiza bunge;multiple active components;wave-length switching
- 0
- |
- 259
- |
- 7
发布时间：2024-09-23 -
Determination of Diosgenin in Paris polyphylla var. yunnanensis at Different Collecting Time by HPLC
摘要：Objective:To determine diosgenin in Paris polyphylla var.yunnanensis at different collecting time.Method:Diosgenin was separated on a Zorbax SB-C18（4.6 mm × 150 mm,5 μm） column and detected at 203 nm at the flow rate of 1.0 mL.min-1 using acetonitrile-water（90∶ 10） as the mobile phase.Column tempera-ture was at 35 ℃.Result: There were differences in the contents of diosgenin in P.polyphylla var.yunnanensis at different collecting time.The contents of diosgenin increased from April to June and reached the highest level in May and June.However,it decreased sharply in June and July.The contents maintained the same level from July to October.Conclusion: The contents of diosgenin in P.polyphylla var.yunnanensis varied with the collecting seasons.关键词：Paris polyphylla var.yunnanensis;diosgenin;HPLC;different collecting time;determination- 0
- |
- 585
- |
- 34
发布时间：2024-09-23 - 摘要：Objective: To determine diosgenin in Paris polyphylla var. yunnanensis at different collecting time. Method: Diosgenin was separated on a Zorbax SB-C18 (4.6 mm×150 mm, 5 μm ) column and detected at 203 nm at the flow rate of 1.0 mL ·min- 1 using acetonitrile-water (90 ∶10) as the mobile phase. Column temperature was at 35 ℃. Result : There were differences in the contents of diosgenin in P. polyphylla var. yunnanensis at different collecting time. The contents of diosgenin increased from April to June and reached the highest level in May and June. However, it decreased sharply in June and July. The contents maintained the same level from July to October. Conclusion : The contents of diosgenin in P. polyphylla var. yunnanensis varied with the collecting seasons.关键词：Paris polyphylla var. yunnanensis;diosgenin;HPLC;different collecting time;determination
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:Gallic acid of Saxifraga stolonifera in Guizhou was determined by HPLC.The difference of the quality of S.stolonifera in Guizhou was studied by the current quality criteria of S.stolonifera.It aims at providing a technological basis of improving the method of controlling the quality of S.stolonifera and the standardi-zation planting.Method:The content of bergenin and gallic acid in S.stolonifera was determined by HPLC.Result: The content of bergenin of S.stolonifera was higher than 0.080%,the minimum of current quality criteria,and the average content of gallic acid was 0.121%.The content of bergenin and gallic acid in S.stolonifera was different from various habitats,various collection time and various processing methods.Conclusion: The quality of S.stolonifera was evaluated by using various componential indices and to provide a standardized planting technological basis for GAP and improve the method of quality control of S.stolonifera.关键词：Saxifraga stolonifera;gallic acid;bergenin;HPLC
- 0
- |
- 175
- |
- 10
发布时间：2024-09-23 - 摘要：Objective: Gallic acid of Saxifraga stolonifera in Guizhou was determined by HPLC. The difference of the quality of S.stolonifera in Guizhou was studied by the current quality criteria of S.stolonifera. It aims at providing a technological basis of improving the method of controlling the quality of S.stolonifera and the standardization planting. Method: The content of bergenin and gallic acid in S.stolonifera was determined by HPLC. Result : The content of bergenin of S.stolonifera was higher than 0.080%, the minimum of current quality criteria, and the average content of gallic acid was 0.121%. The content of bergenin and gallic acid in S.stolonifera was different from various habitats, various collection time and various processing methods. Conclusion : The quality of S.stolonifera was evaluated by using various componential indices and to provide a standardized planting technological basis for GAP and improve the method of quality control of S.stolonifera.关键词：Saxifraga stolonifera;gallic acid;bergenin;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for simultaneous determination of oleanolic acid and ursolic acid from ten fruits of Chinese medicinal herbs, including Fructus Corni, Fructus Crataegi, Fructus Ligustri, Fructus Chaenomelis, Fructus Mume, Fructus Forsythiae, Pericarpium Granati, Calyx Kaki, Spica Prunellae and Fructus Chaenomelis Sinensis, and their chromatograms were compared. Method: Oleanolic acid and ursolic acid were separated on Shim-pack CLC-ODS (M) (4.6 mm×250 mm, 5 μm) with mobile phase consisted of methanol-water-glacial acetic acid-triethylamine (265 ∶35 ∶0.1 ∶0.05) at a flow rate of 0.6 mL ·min-1. The detective wavelength was set at 210 nm by photodiode array detector (PDAD). Result : The total contents of oleanolic acid and ursolic acid from Fructus Forsythiae, Spica Prunellae and Fructus Ligustri were higher than that of others. Some identification characteristics were shown in the analysis of ten kinds of Chinese herbal medicines under the conditions in this paper. Conclusion : This method was simple, accurate and reproducible, and suitable for the analysis of these drugs. The chromatogram information may be useful for the identification of the ten fruits from Chinese medicinal herbs.关键词：fruits of Chinese medinal herbs;oleanolic acid;ursolic acid;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for simultaneous determination of oleanolic acid and ur-solic acid from ten fruits of Chinese medicinal herbs,including Fructus Corni,Fructus Crataegi,Fructus Ligustri,Fructus Chaenomelis,Fructus Mume,Fructus Forsythiae,Pericarpium Granati,Calyx Kaki,Spica Prunellae and Fructus Chaenomelis Sinensis,and their chromatograms were compared.Method:Oleanolic acid and ursolic acid were separated on Shim-pack CLC-ODS（M）（4.6 mm × 250 mm,5 μm） with mobile phase consisted of methanol-water-glacial acetic acid-triethylamine（265∶ 35∶ 0.1∶ 0.05） at a flow rate of 0.6 mL.min-1.The detective wavelength was set at 210 nm by photodiode array detector（PDAD）.Result: The total contents of oleanolic acid and ursolic acid from Fructus Forsythiae,Spica Prunellae and Fructus Ligustri were higher than that of others.Some identification characteristics were shown in the analysis of ten kinds of Chinese herbal medicines under the conditions in this paper.Conclusion: This method was simple,accurate and reproducible,and suitable for the analysis of these drugs.The chromatogram information may be useful for the identification of the ten fruits from Chinese medicinal herbs.关键词：fruits of Chinese medinal herbs;oleanolic acid;ursolic acid;HPLC
- 0
- |
- 557
- |
- 29
发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for the determination of tetrahydropalmatine in Xiaoheling capsule. Method: The HPLC separation was performed on C18 column (4.6 mm×150 mm, 5 μm) with mobile phase consisted of acetonitrile -2% acetic acid solution (ajusted pH to 5.0 using triethylamine) (25 ∶75); The detective wavelength was set at 280 nm with a flow rate of 1 mL ·min-1and the column temperature was set at 30 ℃. Result : Corydalis was linear in the range of 44.68-357.44 mg ·L-1 with coefficient 0.999 9. Average recovery was 101.10% with RSD 2.42% (n=5). Conclusion : The method was simple, rapid and accurate, and applicable for the quality control of Xiaoheling capsule.关键词：Xiaoheling capsule;tetrahydropalmatine;HPLC;content determination
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for the determination of tetrahydropalmatine in Xiaoheling capsule.Method:The HPLC separation was performed on C18 column（4.6 mm × 150 mm,5 μm） with mobile phase consisted of acetonitrile-2% acetic acid solution（ajusted pH to 5.0 using triethylamine）（25∶ 75）;The detective wavelength was set at 280 nm with a flow rate of 1 mL.min-1and the column temperature was set at 30 ℃.Result: Corydalis was linear in the range of 44.68-357.44 mg.L-1 with coefficient 0.999 9.Average recovery was 101.10% with RSD 2.42%（n = 5）.Conclusion: The method was simple,rapid and accurate,and applicable for the quality control of Xiaoheling capsule.关键词：Xiaoheling capsule;tetrahydropalmatine;HPLC;content determination
- 0
- |
- 52
- |
- 4
发布时间：2024-09-23 - 摘要：目的:建立了石斛明目丸的薄层色谱鉴别与含量测定方法。方法:修订了显微鉴别项,增加了人参、黄连、川芎的薄层鉴别项,并采用高效液相色谱法测定黄连中盐酸小檗碱的含量。结果:薄层色谱斑点清晰,重复性好,盐酸小檗碱在0.010 22～0.306 6μg线性关系良好。平均回收率为97.71%,RSD为1.0%。结论:本方法简便易行,重复性好。关键词：石斛明目丸;薄层色谱;高效液相色谱;盐酸小檗碱
- 0
- |
- 153
- |
- 10
发布时间：2024-09-23 - 摘要：Objective:To study the qualitative and quantitative methods of Sixiaowan pill.Method:Radix et Rhizoma Rhei,Rhizoma Cyperi,Crataegus pinnatifida and Areca catechu in Sixiaowan pill were identified with the method of TLC.The contents of emodin and chrysophanol were determined with the method of HPLC.Result: For TLC,the spots of four herbal drugs were well separated and without interference.Emodin and chrysophanol were linear in the range of 0.051 3-0.256 5μg and 0.153 9-0.769 5μg respectively.Conclusion: The methods set up by this study were accurate and easy to perform with the merits of good resolution,specificity and reproducibili-ty.It could effectively control the quality of Sixiaowan pill.关键词：Sixiaowan pill;TLC;HPLC
- 0
- |
- 114
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To study the qualitative and quantitative methods of Sixiaowan pill. Method: Radix et Rhizoma Rhei, Rhizoma Cyperi, Crataegus pinnatifida and Areca catechu in Sixiaowan pill were identified with the method of TLC. The contents of emodin and chrysophanol were determined with the method of HPLC. Result : For TLC, the spots of four herbal drugs were well separated and without interference. Emodin and chrysophanol were linear in the range of 0.051 3-0.256 5μg and 0.153 9-0.769 5μg respectively. Conclusion: The methods set up by this study were accurate and easy to perform with the merits of good resolution, specificity and reproducibility. It could effectively control the quality of Sixiaowan pill.关键词：Sixiaowan pill;TLC;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a determination method for quality control of Rougan capsule.Method: The contents of salvianolic acid B,curcumin,demethoxycurcumin and bisdemethoxycurcumin in Rougan capsule were determined by HPLC-DAD.These compounds were analyzed with a Apollo C18 column（4.6 mm × 250 mm,5 μm） by gradient elution using 4% acetic acid-acetonitrile as the mobile phase,the flow rata was 1.0 mL.min-1,the column temperature was maintained at 30 ℃ and the wavelength was detected at 286 and 426 nm.The content of tauroursodeoxycholic acid in Rougan capsule was determined by HPLC-ELSD.It was analyzed with a Apollo C18 column（4.6 mm × 250 mm,5 μm） by isocratic elution using acetonitrile-acetic acid-ammonium acetate solution（pH 4.5） as the mobile phase,the flow rate was 0.8 mL.min-1,the temperature of the drift tube was set at 80℃,the pressure of nebulizing gas（air） was at 35 psi,the temperature of the atomizer was at 36 ℃.Result: All these compounds were linear in the range of 0.148 0-1.850,0.125 6-1.570,0.043 2-0.540 0,0.033 6-0.420 0 and 0.972 0-12.15 μg（r = 0.999 3-0.999 9）,respectively.The RSDs of the precision,reproducibility and stability test were less than 3%;the average recovery of the method was in the range of 97.8%-101.7%.Conclusion: The established method is simple,accurate,and repeatable and can be used for quality control of Rougan capsule.关键词：Rougan capsule;salvianolic acid B;curcumin constituents;tauroursodexycholic acid;HPLC;determination
- 0
- |
- 137
- |
- 2
发布时间：2024-09-23 - 摘要：Objective: To establish a determination method for quality control of Rougan capsule. Method: The contents of salvianolic acid B, curcumin, demethoxycurcumin and bisdemethoxycurcumin in Rougan capsule were determined by HPLC-DAD. These compounds were analyzed with a Apollo C18 column (4.6 mm×250 mm, 5 μm) by gradient elution using 4% acetic acid-acetonitrile as the mobile phase, the flow rata was 1.0 mL ·min-1, the column temperature was maintained at 30 ℃ and the wavelength was detected at 286 and 426 nm. The content of tauroursodeoxycholic acid in Rougan capsule was determined by HPLC-ELSD. It was analyzed with a Apollo C18 column (4.6 mm×250 mm, 5 μm) by isocratic elution using acetonitrile-acetic acid-ammonium acetate solution (pH 4.5) as the mobile phase, the flow rate was 0.8 mL ·min-1, the temperature of the drift tube was set at 80℃, the pressure of nebulizing gas (air) was at 35 psi, the temperature of the atomizer was at 36 ℃. Result : All these compounds were linear in the range of 0.148 0-1.850, 0.125 6-1.570, 0.043 2-0.540 0, 0.033 6-0.420 0 and 0.972 0-12.15 μg (r=0.999 3-0.999 9), respectively. The RSDs of the precision, reproducibility and stability test were less than 3%; the average recovery of the method was in the range of 97.8%-101.7%. Conclusion : The established method is simple, accurate, and repeatable and can be used for quality control of Rougan capsule.关键词：Rougan capsule;salvianolic acid B;curcumin constituents;tauroursodexycholic acid;HPLC;determination
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To establish a method for determination of matrine in Shuyin Lotion. Method: The matrine content was determined by HPLC on inertsil ODS-SP column (4.6 mm×150 mm, 5 μm) with a mobile phase of acetonitrile-water-triethylamine (3 ∶97 ∶0.2, adjusted to pH 3.5 with phosphoric acid), the flow rate was 1.0 mL ·min-1, and the detection wavelength was set at 220 nm. Result : Matrine was linear in range of 3.9-117.0 μg (r=0.999 9). The average recovery was 101.69% with RSD of 1.84% (n=9). Conclusion : The method is simple, quick and accurate. It can be used for the quality control of Shuyin lotion.关键词：Shuyin lotion;matrine;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a method for determination of matrine in Shuyin Lotion.Method:The matrine content was determined by HPLC on inertsil ODS-SP column（4.6 mm × 150 mm,5 μm） with a mobile phase of acetonitrile-water-triethylamine（3∶ 97∶ 0.2,adjusted to pH 3.5 with phosphoric acid）,the flow rate was 1.0 mL.min-1,and the detection wavelength was set at 220 nm.Result: Matrine was linear in range of 3.9-117.0 μg（r =0.999 9）.The average recovery was 101.69% with RSD of 1.84%（n =9）.Conclusion: The method is simple,quick and accurate.It can be used for the quality control of Shuyin lotion.关键词：Shuyin lotion;matrine;HPLC
- 0
- |
- 61
- |
- 9
发布时间：2024-09-23 - 摘要：Objective: To study the chemical constituents of Maimendong plus Qianjin Weijing decoction. Method: The constituents were isolated by column chromatography and their structures were elucidated by physico-chemical properties and spectroscopic analysis. Result : Twelve compounds were isolated and identified as formononetin (1), liquiritigenin (2), isoliquiritigenin (3), 20(R)-ginsenoside Rg3 (4), succinic acid (5), isovanillic acid (6), uracil (7), succinic anhydride (8), D-mannitol (9), p-hydroxybenzoic acid (10), β-sitosterol (11), stigmasterol (12). Conclusion : Most of the constituents were come from Radix Glycyrrhiza, Radix Ginseng, Rhizoma Pinelliae and Rhizoma Phragmites, and compounds 1-4 may be responsible for lung cancer therapeutic action of the formula on the basis of literature reports.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents of Maimendong plus Qianjin Weijing decoction.Method:The constituents were isolated by column chromatography and their structures were elucidated by physico-chemical properties and spectroscopic analysis.Result: Twelve compounds were isolated and identified as form-ononetin（1）,liquiritigenin（2）,isoliquiritigenin（3）,20（R）-ginsenoside Rg3（4）,succinic acid（5）,isovanil-lic acid（6）,uracil（7）,succinic anhydride（8）,D-mannitol（9）,p-hydroxybenzoic acid（10）,β-sitosterol（11）,stigmasterol（12）.Conclusion: Most of the constituents were come from Radix Glycyrrhiza,Radix Gin-seng,Rhizoma Pinelliae and Rhizoma Phragmites,and compounds 1-4 may be responsible for lung cancer therapeu-tic action of the formula on the basis of literature reports.
- 0
- |
- 307
- |
- 14
发布时间：2024-09-23 - 摘要：The purpose was to screen antibacterial constituent from the barks of Pterocarya stenoptera,which was a Chinese traditional drug often used to treat bacterial and fungous skin diseases for hundreds of years.Through extraction,precipitation,silica gel column adsorption chromatography,Sephadex LH-20 gel chromatogra-phy using a bioassay-guided fractionation technique（An antibacterial experiment in vitro was used as the screening technique）,a red needlelike liposoluble compound with excellent antibacterial activity to Diplococcus cata,Strepto-coccus albus,Gibberella saubinetii,Salmonella sp.,Bacillus subtilis,and so on,was isolated from the barks of P.stenoptera.The compound was identified as 2-ethoxy-5-hydroxynaphthalene-1,4-dione（C12H10O4,M = 218） by EI-MS,1H-NMR,13C-NMR and DEPT.The structure of another antibacterial compound,juglone（Azadbakht M,2005）,is very similar to 2-ethoxy-5-hydroxynaphthalene-1,4-dione except for a 2-ethoxy,which suggest that this kind of chemical structure may has potential value for development and application of new antibacterial compound.The results in this paper had not only revealed the antibacterial activity substance of of P.stenoptera,but also laid the foundation for the study of quality standard of P.stenoptera and antibacterial active section for externally used.关键词：Pterocarya stenoptera;antibacterial activity;bioassay-guided fractionation technique;isola-tion and purification;NMR;EI-MS;naphthoquinone
- 0
- |
- 316
- |
- 14
发布时间：2024-09-23 - 摘要：The purpose was to screen antibacterial constituent from the barks of Pterocarya stenoptera, which was a Chinese traditional drug often used to treat bacterial and fungous skin diseases for hundreds of years. Through extraction, precipitation, silica gel column adsorption chromatography, Sephadex LH-20 gel chromatography using a bioassay-guided fractionation technique (An antibacterial experiment in vitro was used as the screening technique), a red needlelike liposoluble compound with excellent antibacterial activity to Diplococcus cata, Streptococcus albus, Gibberella saubinetii, Salmonella sp., Bacillus subtilis, and so on, was isolated from the barks of P. stenoptera. The compound was identified as 2-ethoxy-5-hydroxynaphthalene-1,4-dione (C12H10O4, M=218) by EI-MS, 1H-NMR, 13C-NMR and DEPT. The structure of another antibacterial compound, juglone (Azadbakht M, 2005), is very similar to 2-ethoxy-5-hydroxynaphthalene-1,4-dione except for a 2-ethoxy, which suggest that this kind of chemical structure may has potential value for development and application of new antibacterial compound. The results in this paper had not only revealed the antibacterial activity substance of of P. stenoptera, but also laid the foundation for the study of quality standard of P. stenoptera and antibacterial active section for externally used.关键词：Pterocarya stenoptera;antibacterial activity;bioassay-guided fractionation technique;isolation and purification;NMR;EI-MS;naphthoquinone
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To investigate the chemical constituents of Celastrus vamotii. Method: The air-dried roots and stems of C.vamotii were powdered and refluxed with 95% EtOH for three times. The combined extract were evaporation under reduced pressure to give residue, which was suspended in water and fractionated with ethyl acetate. Result : Five compounds were isolated from the EtOAc fraction and identified on basis of spectral and chemical evidence such as MS, 1H-NMR, and 13C-NMR etc. They are β-sitosterol (1), β-amyrin (2),β-amyrin acetate (3), 30-hydroxylup-20 (29)-en-3-one (4),and 3-oxolup-20-(29)-en-30-al (5). Conclusion: All compounds were isolated from the plant for the first time.关键词：Celastrus vamotii;Cleastrus;chemical constituents;triterpenoids
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the chemical constituents of Celastrus vamotii.Method:The air-dried roots and stems of C.vamotii were powdered and refluxed with 95% EtOH for three times.The combined extract were evaporation under reduced pressure to give residue,which was suspended in water and fractionated with ethyl acetate.Result: Five compounds were isolated from the EtOAc fraction and identified on basis of spectral and chemical evidence such as MS,1H-NMR,and 13C-NMR etc.They are β-sitosterol（1）,β-amyrin（2）,β-amyrin acetate（3）,30-hydroxylup-20（29）-en-3-one（4）,and 3-oxolup-20-（29）-en-30-al（5）.Conclusion: All com-pounds were isolated from the plant for the first time.关键词：Celastrus vamotii;Cleastrus;chemical constituents;triterpenoids
- 0
- |
- 127
- |
- 2
发布时间：2024-09-23 - 摘要：Objective: To get a crude perception of the composition and the molecular weight of Salvia miltiorrhiza polysaccharides. Method: Using high perform HPGPC and alcohol fractionation,We got a better outline of the composition of the polysaccharides and respectively analized their molecular weights. Result : The test results proved that S.miltiorrhiza polysaccharides breaks down to two parts with respect to their ranges of molecular weight. Conclusion : The part with low molecular weight exhibits the character of pure substance which is exploitable.关键词：HPGPC;M w;Salvia miltiorrhiza polysaccharides(SMP);alcohol fractionation
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To get a crude perception of the composition and the molecular weight of Salvia miltiorrhiza polysaccharides.Method:Using high perform HPGPC and alcohol fractionation,We got a better outline of the composition of the polysaccharides and respectively analized their molecular weights.Result: The test results proved that S.miltiorrhiza polysaccharides breaks down to two parts with respect to their ranges of molecular weight.Conclusion: The part with low molecular weight exhibits the character of pure substance which is exploitable.关键词：HPGPC;Mw;Salvia miltiorrhiza polysaccharides（SMP）;alcohol fractionation
- 0
- |
- 345
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: To study DPPH and ABTS radical scavenging activities of Oxtropis myriophylla. Method: The anti-oxidant activity of O . myriophylla was measured by DPPH assay and ABTS assay. Furthermore, the AB-8 macroporous absorbent resincolumn chromatography with gradient elution (aqueous solution, 50% and 90% ethanol solution)were used. Result: 50% ethanol extract from O . myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions, which is a trifle stronger than ascorbic acid. In addition, the active ingredient was screened effectively by the column chromatography method. Conclusion: 50% ethanol extract from O . myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions, which is a trifle stronger than ascorbic acid. In addition, the active ingredient was screened effectively by the column chromatography method. Conclusion: The 50% ethanol extracts exhibited strong scavenging capacity of free radical.关键词：Oxtropis myriophylla;scavenging capacity of free radical;anti-oxidant
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study DPPH and ABTS radical scavenging activities of Oxtropis myriophylla.Method: The anti-oxidant activity of O.myriophylla was measured by DPPH assay and ABTS assay.Furthermore,the AB-8 macroporous absorbent resincolumn chromatography with gradient elution（aqueous solution,50% and 90% ethanol solution）were used.Result:50% ethanol extract from O.myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions,which is a trifle stronger than ascorbic acid.In addition,the active ingredient was screened effectively by the column chromatography method.Conclusion:50% ethanol extract from O.myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions,which is a trifle stronger than ascorbic acid.In addition,the active ingredient was screened effectively by the column chromatography method.Conclusion: The 50% ethanol extracts exhibited strong scavenging capacity of free radical.关键词：Oxtropis myriophylla;scavenging capacity of free radical;anti-oxidant
- 0
- |
- 239
- |
- 8
发布时间：2024-09-23 - 摘要：Objective:To determine gallic acid and quercetin from different parts of Archidendron clypearia,and to provide evidence for the rational development and utilization of A.clypearia.Method: HPLC method was used with Kromosil C18column（4.6 mm × 250 mm,5 μm）,and the mobile phases were acetonitrile and 0.2% phosphoric acid solution with the gradient elution.The flow rate was 1 mL.min-1 and the detection wavelength was 254 nm.Result: Both of the content of gallic acid and quercetin are highest in the leaves,lower in the new shoot and lowest in the shoot,even quercetin cannot be tested in the shoot.Conclusion: The method can be used to control the quality of Archidendron clypearia.It is simple,accurate,reproducible and suitable for simultaneous determination of two active constituents in Archidendron clypearia.
- 0
- |
- 275
- |
- 14
发布时间：2024-09-23 - 摘要：Objective: To determine gallic acid and quercetin from different parts of Archidendron clypearia, and to provide evidence for the rational development and utilization of A.clypearia. Method: HPLC method was used with Kromosil C18 column (4.6 mm×250 mm, 5 μm), and the mobile phases were acetonitrile and 0.2% phosphoric acid solution with the gradient elution. The flow rate was 1 mL ·min-1 and the detection wavelength was 254 nm. Result: Both of the content of gallic acid and quercetin are highest in the leaves,lower in the new shoot and lowest in the shoot, even quercetin cannot be tested in the shoot. Conclusion: The method can be used to control the quality of Archidendron clypearia. It is simple, accurate, reproducible and suitable for simultaneous determination of two active constituents in Archidendron clypearia.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: Use the metabonomics method to study the CCl4-induced liver injury model, and discriminate the potential biomarkers contributed to liver injury in plasma metabolites. Method: Adopt the metabolic skills based on UPLC-TOF-MS and PCA, to research the change of plasma metabolite in normal rat or CCl4-induced liver injury rat with two different doses. Result: Ascertain the CCl4 dosage in establish rat liver injury model of plasma metabolites and find ten characteristic biomakers in plasma. Conclusion: Metabonomics method was successfully used to study the CCl4-induced liver injury model by the rat plasma, and laid a foundation for the further study of the liver protection of Traditional Chinese Medicine.关键词：metabonomics;UPLC-TOF-MS;liver injury;rat plasma;biomarker
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:Use the metabonomics method to study the CCl4-induced liver injury model,and discriminate the potential biomarkers contributed to liver injury in plasma metabolites.Method:Adopt the metabolic skills based on UPLC-TOF-MS and PCA,to research the change of plasma metabolite in normal rat or CCl4-induced liver injury rat with two different doses.Result: Ascertain the CCl4 dosage in establish rat liver injury model of plasma metabolites and find ten characteristic biomakers in plasma.Conclusion: Metabonomics method was successfully used to study the CCl4-induced liver injury model by the rat plasma,and laid a foundation for the further study of the liver protection of Traditional Chinese Medicine.关键词：metabonomics;UPLC-TOF-MS;liver injury;rat plasma;biomarker
- 0
- |
- 697
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: To sutdy the enzyme-reducing action and pharmacodynamics of Xiaoyao powder on liver injury in rats in the morning and evening in order to provide chronomedication evidence for hepatopaths. Method: The liver injury rat model was developed by subcutaneous injection of forty percent carbon tetrachloride every two days.With the decrease of aspartate amino transferase as the parameter,the pharmacodynamics in the morning and evening of Xiaoyao powder was studied.The apparent parameters of pharmacodynamics were estimated based on the time-effect curve by PKSolver 2.0. Result: The time-effect curve of Xiaoyao powder shows characteristics of double peaks and can be described by statistical moment.The main pharmacodynamic parameters are as follows: td=17.79h(day),24.30h(night),t1/2(ED)=3.73h(day),3.50h(night),AUC=657.07U ·L-1 ·h-1(day),810.19U ·L-1 ·h-1(night),MRT=6.23h(day),6.19h (night), respectively. Conclusion: Significant circadian rhythm of pharmacodynamics was found in rats when Xiaoyao powder was given at different times of the day.The findings suggest that the hepatic protection effect for liver injury of Xiaoyao powder in rats is better when given at the end of rest phase than other time session.关键词：Xiaoyao Powder;pharmacodynamics;circadian rhythm;liver injury
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To sutdy the enzyme-reducing action and pharmacodynamics of Xiaoyao powder on liver injury in rats in the morning and evening in order to provide chronomedication evidence for hepatopaths.Method:The liver injury rat model was developed by subcutaneous injection of forty percent carbon tetrachloride every two days.With the decrease of aspartate amino transferase as the parameter,the pharmacodynamics in the morning and evening of Xiaoyao powder was studied.The apparent parameters of pharmacodynamics were estimated based on the time-effect curve by PKSolver 2.0.Result: The time-effect curve of Xiaoyao powder shows characteristics of double peaks and can be described by statistical moment.The main pharmacodynamic parameters are as follows: td = 17.79 h（day）,24.30 h（night）,t1/2（ED） = 3.73 h（day）,3.50 h（night）,AUC = 657.07 U.L-1.h-1（day）,810.19 U.L-1.h-1（night）,MRT = 6.23 h（day）,6.19 h（night）,respectively.Conclusion: Significant circadian rhythm of pharmacodynamics was found in rats when Xiaoyao powder was given at different times of the day.The findings suggest that the hepatic protection effect for liver injury of Xiaoyao powder in rats is better when given at the end of rest phase than other time session.关键词：Xiaoyao Powder;pharmacodynamics;circadian rhythm;liver injury
- 0
- |
- 143
- |
- 2
发布时间：2024-09-23 - 摘要：Objective:To develop an HPLC-FLU method for the determination of capsaicin in rabbit plasma and to study its pharmacokinetics.Method: Six rabbits were given capsaicin nanoemulsions and ethanol solution（1mg.kg-1） via ear vein by double cycle crossover design,and after 3,6,9,12,15,25,40,60 and 120 min,blood samples were collected.HPLC-FLU was established to determine the plasma concentration of capsaicin and the pharmacokinetic evaluation was carried out.Result:The linear range of capsaicin was 0.197-49.3 mg.L-1（r = 0.999 6） and the limit of detection of capsaicin was 5.9 ng.mL-1,the average recovery was 102.5% ～ 107.6% with RSD < 5%.The t1/2 of capsaicin nanoemulsions and solution was（11.77 ± 2.32） min and（9.53 ± 2.19） min respectively,and the AUC was（5 311 ± 1 324.6） ng.mL-1.kg-1.min and（4 909.5 ± 1 110.4） ng.mL-1.kg-1.min respectively.Conclusion:This assay method is simple,precise and sensitive enough for the pharmacokinetic study of capsaicin.The pharmacokinectics parameters between capsaicin nanoemulsions and solution had no significant difference（t test,P > 0.05）.关键词：capsaicin;pharmacokinetics;HPLC-FLU;nanoemulsions
- 0
- |
- 168
- |
- 7
发布时间：2024-09-23 - 摘要：Objective: To develop an HPLC-FLU method for the determination of capsaicin in rabbit plasma and to study its pharmacokinetics. Method: Six rabbits were given capsaicin nanoemulsions and ethanol solution (1mg ·kg-1) via ear vein by double cycle crossover design, and after 3, 6, 9, 12, 15, 25, 40, 60 and 120 min, blood samples were collected. HPLC- FLU was established to determine the plasma concentration of capsaicin and the pharmacokinetic evaluation was carried out. Result: The linear range of capsaicin was 0.197-49.3 mg ·L-1(r=0.999 6) and the limit of detection of capsaicin was 5.9 ng ·mL-1, the average recovery was 102.5%~107.6% with RSD<5%. The t1/2 of capsaicin nanoemulsions and solution was (11.77±2.32) min and (9.53±2.19) min respectively, and the AUC was (5 311±1 324.6) ng ·mL-1 ·kg-1 ·min and (4 909.5±1 110.4) ng ·mL-1 ·kg-1 ·min respectively. Conclusion: This assay method is simple, precise and sensitive enough for the pharmacokinetic study of capsaicin. The pharmacokinectics parameters between capsaicin nanoemulsions and solution had no significant difference ( t test, P>0.05).关键词：capsaicin;pharmacokinetics;HPLC-FLU;nanoemulsions
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of Siwu decoction and its composite drug-pairs or drugs on the primary dysmenorrheal model of mouse so as to clarify the potential mechanism and compatible regularity of Siwu decoction. Method: The model of primary dysmenorrheal mice were used to investigate the effect of Siwu decoction on the writhing response and the levels of nitric oxide and calcium ion in mice uterine tissue. Result: Siwu decoction and its composite drug-pairs and drugs had certain pharmacological effect on primary dysmenorrheal by reducing the writhing times, increasing nitric oxide (NO) levels and reducing calcium ion (Ca2+) concentration in uterine tissue of mice. Conclusion: Siwu decoction and its composite drug-pairs and drugs exhibited characteristic influence on the primary dysmenorrheal model of mouse. The drug of Rehmanniae Radix Praeparata might have the antagonistic effect to Chuanxiong Rhizoma, Angelicar Sinensis Radix and Paeoniae Radix Alba on the activity of reducing the model mice’s writhing times. The drug-pair of Angelicar Sinensis Radix and Paeoniae Radix Alba may be the most significant drug-pair for the treatment effect on the model of primary dysmenorrheal mice. This study provided a reliable reference for the follow-up study.关键词：SIWU decoction;primary dysmenorrheal;nitric oxide;calcium ion
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the influence of Siwu decoction and its composite drug-pairs or drugs on the primary dysmenorrheal model of mouse so as to clarify the potential mechanism and compatible regularity of Siwu decoction.Method: The model of primary dysmenorrheal mice were used to investigate the effect of Siwu decoction on the writhing response and the levels of nitric oxide and calcium ion in mice uterine tissue.Result:Siwu decoction and its composite drug-pairs and drugs had certain pharmacological effect on primary dysmenorrheal by reducing the writhing times,increasing nitric oxide（NO） levels and reducing calcium ion（Ca2 +） concentration in uterine tissue of mice.Conclusion:Siwu decoction and its composite drug-pairs and drugs exhibited characteristic influence on the primary dysmenorrheal model of mouse.The drug of Rehmanniae Radix Praeparata might have the antagonistic effect to Chuanxiong Rhizoma,Angelicar Sinensis Radix and Paeoniae Radix Alba on the activity of reducing the model mice’s writhing times.The drug-pair of Angelicar Sinensis Radix and Paeoniae Radix Alba may be the most significant drug-pair for the treatment effect on the model of primary dysmenorrheal mice.This study provided a reliable reference for the follow-up study.关键词：SIWU decoction;primary dysmenorrheal;nitric oxide;calcium ion
- 0
- |
- 702
- |
- 25
发布时间：2024-09-23 - 摘要：Objective: To study the anti-inflammatory effects of the Liangxue Jiedu Huoxue Granules. Method: The anti-inflammatory effect of Liangxue Jiedu Huoxue Granules was observed on pathological model with increasing vascular permeability induced by acetic acid in mice and the model of paw edema caused by egg white in rats. Result: The Liangxue Jiedu Huoxue Granules could significantly decrease the degree of paw edema caused by egg white in rats(P＜0.05) and markly inhibit the degree of the increase in permeability of the capillaries(P＜0.01). Conclusion: The Liangxue Jiedu Huoxue Granules has significant action of anti-inflammation.关键词：Liangxue Jiedu Huoxue Granules;anti-inflammation
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the anti-inflammatory effects of the Liangxue Jiedu Huoxue Granules.Method:The anti-inflammatory effect of Liangxue Jiedu Huoxue Granules was observed on pathological model with increasing vascular permeability induced by acetic acid in mice and the model of paw edema caused by egg white in rats.Result: The Liangxue Jiedu Huoxue Granules could significantly decrease the degree of paw edema caused by egg white in rats（P < 0.05） and markly inhibit the degree of the increase in permeability of the capillaries（P < 0.01）.Conclusion: The Liangxue Jiedu Huoxue Granules has significant action of anti-inflammation.关键词：Liangxue Jiedu Huoxue Granules;anti-inflammation
- 0
- |
- 77
- |
- 3
发布时间：2024-09-23 - 摘要：Objective:To study the protective effect of Yinzhihuang Injection on hepatic cell damage caused by Carbon tetrachloride（CCl4） in vitro.Method: CCl4 fumigation method was adopted to duplicate hepatic cell damage in vitro,and the protective effect of Yinzhihuang Injection and Fufang Yinchen Injection and their main active ingredients,baicalin and coumarin,from different companies were investigated.Result:The ALT,AST and MDA of damaged cells could be decreased and GSH-Px could be elevated by Yinzhihuang Injections from different factories,and the strongest protective effect was originated by Fufang Yinchen Injection.Conclusion: Both Yinzhihuang Injection and their main active ingredients,baicalin and coumarin,could prevent the hepatic cell damage caused by CCl4 in vitro,and the protective effect of normal samples from different companies had good consistency agreed with each other.关键词：Yinzhihuang Injection;Fufang Yinchen Injection;carbon tetrachloride（CCl4）;hepatic cell damage;protective effect
- 0
- |
- 362
- |
- 18
发布时间：2024-09-23 - 摘要：Objective: To study the protective effect of Yinzhihuang Injection on hepatic cell damage caused by Carbon tetrachloride (CCl4) in vitro. Method: CCl4 fumigation method was adopted to duplicate hepatic cell damage in vitro , and the protective effect of Yinzhihuang Injection and Fufang Yinchen Injection and their main active ingredients, baicalin and coumarin, from different companies were investigated. Result: The ALT, AST and MDA of damaged cells could be decreased and GSH-Px could be elevated by Yinzhihuang Injections from different factories, and the strongest protective effect was originated by Fufang Yinchen Injection. Conclusion: Both Yinzhihuang Injection and their main active ingredients, baicalin and coumarin, could prevent the hepatic cell damage caused by CCl4 in vitro, and the protective effect of normal samples from different companies had good consistency agreed with each other.关键词：Yinzhihuang Injection;Fufang Yinchen Injection;carbon tetrachloride(CCl4);hepatic cell damage;protective effect
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To explore the possible functional mechanism for effect of Er-xian Decoction on uterus through quantity analysis of effect of Er-xian Decoction and its compositions on uterus in immature rat. Method: Fifty-four immature female SD rats were randomly divided into nine groups: normal control group(A), diethylstilbestrol(DES) group(B), Er-xian Decoction group(C), Herba Epimedii group(D), Rhizoma Curculiginis group(E), Radix Morindae Officinalis group(F), Radix Angelicae Sinensis group(G), Cortex Phellodendri group(H), Rhizoma Anemarrhenae group(I). After the rats were administrated for six days, serum were got to measure Estradiol(E2). After bilateral uterus was removed and weighed, paraffin sections, HE staining and immunohistochemical staining were made. Result: The uterus coefficient was increased significantly in Group B, C, D and E (P<0.05). The level of E2 in Group B only was increased. It was found diethylstilbestrol (DES) could induce increasing in the height of endometrium epidemic cells and the height of endometrium sreoma cells. Immunohistochemical staining showed that DES caused a decrease in the expression of ERα and ERβ in the uterus of immature rats, but Er-xian Decoction and its kidney-warming composition (Herba Epimedii, Rhizoma Curculiginis ) could up-regulate the ERα and ERβ expression. Conclusion: Er-xian Decoction and its kidney-warming composition (Herba Epimedii, Rhizoma Curculiginis ) could act as the phytoestrogen by upregulating the expression of ERα and ERβ in the uterus of immature rats.关键词：Er-xian Decoction;Uterus;estrogen receptor;rat
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To explore the possible functional mechanism for effect of Er-xian Decoction on uterus through quantity analysis of effect of Er-xian Decoction and its compositions on uterus in immature rat.Method:Fifty-four immature female SD rats were randomly divided into nine groups: normal control group（A）,diethylstilbestrol（DES） group（B）,Er-xian Decoction group（C）,Herba Epimedii group（D）,Rhizoma Curculiginis group（E）,Radix Morindae Officinalis group（F）,Radix Angelicae Sinensis group（G）,Cortex Phellodendri group（H）,Rhizoma Anemarrhenae group（I）.After the rats were administrated for six days,serum were got to measure Estradiol（E2）.After bilateral uterus was removed and weighed,paraffin sections,HE staining and immunohistochemical staining were made.Result:The uterus coefficient was increased significantly in Group B,C,D and E（P < 0.05）.The level of E2 in Group B only was increased.It was found diethylstilbestrol（DES） could induce increasing in the height of endometrium epidemic cells and the height of endometrium sreoma cells.Immunohistochemical staining showed that DES caused a decrease in the expression of ERα and ERβ in the uterus of immature rats,but Er-xian Decoction and its kidney-warming composition（Herba Epimedii,Rhizoma Curculiginis） could up-regulate the ERα and ERβ expression.Conclusion: Er-xian Decoction and its kidney-warming composition（Herba Epimedii,Rhizoma Curculiginis） could act as the phytoestrogen by upregulating the expression of ERα and ERβ in the uterus of immature rats.关键词：Er-xian Decoction;Uterus;estrogen receptor;rat
- 0
- |
- 326
- |
- 25
发布时间：2024-09-23 - 摘要：目的:通过经典药理学实验方法,探讨鼻康喷雾剂治疗变应性鼻炎的抗过敏作用。方法:采用变应性鼻炎的常用试验法即大鼠被动皮肤过敏反应试验法,试验中设阳性对照组、空白组、及鼻康喷雾剂的高、中、低剂量组,以生理盐水为空白,鼻炎康片为阳性对照药。结果:通过测定吸收度（A）值,计算鼻康喷雾剂的疗效。其中空白组、阳性对照组、鼻康喷雾剂高、中、低剂量组A值分别为（0.32±0.13）和（0.26±0.06）,（0.21±0.05）和（0.19±0.05）,（0.19±0.11）和（0.15±0.09）,（0.25±0.12）和（0.19±0.09）,（0.30±0.15）和（0.20±0.11）,与空白组比较有显著性差异（P<0.05或P<0.01）。结论:鼻康喷雾剂对大鼠同种被动皮肤过敏反应引起的血管通透性增加有抑制作用,提示有抗过敏作用。关键词：鼻康喷雾剂;抗过敏;大鼠被动皮肤过敏
- 0
- |
- 142
- |
- 8
发布时间：2024-09-23 - 摘要：Objective: To investigate the utilization of grey relational clustering method in evaluating the treatment of Buzhong Yiqi Tang on rats with Qi-deficiency and fever. Method: SD rats were randomly divided into normal group, Qi-deficiency group, Qi-deficiency and fever group, positive control group (aspirin), high-dose Buzhong Yiqi Tang group, middle-dose Buzhong Yiqi Tang group and low-dose Buzhong Yiqi Tang group. Swimming strain method and dietary restriction method were utilized to establish the rat model of Qi-deficiency, addition to normal group and Qi-deficiency group, in each group we established the rat model of Qi-deficiency and fever by intraperitoneal injection of LPS. The difference ΔT between rats’ measuring temperature and BBT of each group was then compared. Finally, using gray relational clustering method a comprehensive evaluation of the experimental results was conducted. Result: As for the treatment on rats with Qi-deficiency and fever, aspirin group and high-dose Buzhong Yiqi Tang group were the best, middle-dose Buzhong Yiqi Tang group and low-dose Buzhong Yiqi Tang group ranked behind. Conclusion: Grey relational clustering method can evaluate the effect of Buzhong Yiqi Tang on rats of Qi-deficiency with fever objectively and fairly.关键词：grey relational clustering method;Buzhong Yiqi Tang;Qi-deficiency with fever
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the utilization of grey relational clustering method in evaluating the treatment of Buzhong Yiqi Tang on rats with Qi-deficiency and fever.Method:SD rats were randomly divided into normal group,Qi-deficiency group,Qi-deficiency and fever group,positive control group（aspirin）,high-dose Buzhong Yiqi Tang group,middle-dose Buzhong Yiqi Tang group and low-dose Buzhong Yiqi Tang group.Swimming strain method and dietary restriction method were utilized to establish the rat model of Qi-deficiency,addition to normal group and Qi-deficiency group,in each group we established the rat model of Qi-deficiency and fever by intraperitoneal injection of LPS.The difference ΔT between rats’measuring temperature and BBT of each group was then compared.Finally,using gray relational clustering method a comprehensive evaluation of the experimental results was conducted.Result:As for the treatment on rats with Qi-deficiency and fever,aspirin group and high-dose Buzhong Yiqi Tang group were the best,middle-dose Buzhong Yiqi Tang group and low-dose Buzhong Yiqi Tang group ranked behind.Conclusion:Grey relational clustering method can evaluate the effect of Buzhong Yiqi Tang on rats of Qi-deficiency with fever objectively and fairly.关键词：grey relational clustering method;Buzhong Yiqi Tang;Qi-deficiency with fever
- 0
- |
- 297
- |
- 29
发布时间：2024-09-23 - 摘要：Objective:To optimize the extraction techniques of Geli Weiyanxiao.Method:The selection of optimum proess was investigated by L9（34） orthogonal design with the Geli Weiyanxiao as the observation index.Result:Water extraction process: to smash the best size for coarse particles,plus 10 times the volume of water,from 1 hour,3 times.Ethanol extraction process: to smash the best size for 10 mesh number,adding10-fold 70% ethanol,eatracting for 2 times,2 hours for each extraction;In consideration of cost-saving and the results of various indexes,the optimal process condition was selected as follows: the best size for coarse particles,plus 10 times the volume of water,from 1 hour,3 times.Conclusion:The optimal extraction technique for Geli Weiyanxiao is stable and reliable.关键词：Geli Weiyanxiao;extraction technique;analgesic effect;anti-inflammatory action
- 0
- |
- 96
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To optimize the extraction techniques of Geli Weiyanxiao. Method: The selection of optimum proess was investigated by L9(34) orthogonal design with the Geli Weiyanxiao as the observation index. Result: Water extraction process: to smash the best size for coarse particles, plus 10 times the volume of water, from 1 hour, 3 times. Ethanol extraction process: to smash the best size for 10 mesh number, adding10-fold 70% ethanol, eatracting for 2 times, 2 hours for each extraction; In consideration of cost-saving and the results of various indexes, the optimal process condition was selected as follows: the best size for coarse particles, plus 10 times the volume of water, from 1 hour, 3 times. Conclusion: The optimal extraction technique for Geli Weiyanxiao is stable and reliable.关键词：Geli Weiyanxiao;extraction technique;analgesic effect;anti-inflammatory action
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To observe the effects of Promoting Pedo-digestion Granules (PPD) on the digestion and absorption function and its maximum torerance dose (MTD). Method: The distance of ink moving in small intestine in KM mice was used to evaluate the effect. The mice were randomly divided into treatment groups, control groups and the difenoxin model control group. Treatment groups were fed with 1,2, 4 g ·kg-1 dosage accordingly. The body weight, food intakes, food efficiency ratio and the secretion amount of SD rats’gastric juice and pepsin activity were also used to evaluate the effect. The rats were randomly divided into treatment groups and control groups. Treatment groups were fed with dosage as the above. PPD were fed for 30 days. MTD was measured. Result: PPD could increase the rate of ink promoting, which was superior to that in the difenoxin model control group, PPD also could increase the body weight of rats, appetite and food exploitation rate, promote the secretion amount of gastric juice and pepsin, pepsin activity, which was superior to that in the control group. MTD was 56 g ·kg-1 as about 54 times of population recommended amount. Conclusion: PPD has the function of promoting the digestion and absorption function, and oral dose commonly used is safe.关键词：Promoting Pedo-digestion Granules;intestine movement;pepsin;maximum torerance dose
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Promoting Pedo-digestion Granules（PPD） on the digestion and absorption function and its maximum torerance dose（MTD）.Method: The distance of ink moving in small intestine in KM mice was used to evaluate the effect.The mice were randomly divided into treatment groups,control groups and the difenoxin model control group.Treatment groups were fed with 1,2,4 g.kg-1 dosage accordingly.The body weight,food intakes,food efficiency ratio and the secretion amount of SD rats’gastric juice and pepsin activity were also used to evaluate the effect.The rats were randomly divided into treatment groups and control groups.Treatment groups were fed with dosage as the above.PPD were fed for 30 days.MTD was measured.Result:PPD could increase the rate of ink promoting,which was superior to that in the difenoxin model control group,PPD also could increase the body weight of rats,appetite and food exploitation rate,promote the secretion amount of gastric juice and pepsin,pepsin activity,which was superior to that in the control group.MTD was 56 g.kg-1 as about 54 times of population recommended amount.Conclusion:PPD has the function of promoting the digestion and absorption function,and oral dose commonly used is safe.关键词：Promoting Pedo-digestion Granules;intestine movement;pepsin;maximum torerance dose
- 0
- |
- 268
- |
- 6
发布时间：2024-09-23 - 摘要：Objective:To investigate the anti-tumor and immune effect of Fuzheng Guben Granule combined with chemotherapy and radiotherapy in tumor-bearing mice.Method:Tumor-bearing model of S180 sarcoma in mice was established by subcutaneous injection of S-180 ascites at armpit.On the next day,the mice were randomly divided into 10 groups,Fuzheng Guben Granule with radio-and chemotherapy was carried out once a day for ten days.Tumor inhibitory percentage,thymus gland,spleen indexes were then measured.The determination of cytokine,such as IL-2,IL-6,TNF-α and peripheral blood cell counts was made.The effect of treatment on tumor inhibition and immunological function was studied.Result:The combined therapy could significantly increase thymus gland,spleen indexes and improved the phagocytic function;antagonize serum IL-2,IL-6 and TNF-α reduction induced by chemotherapy;suppress the decrease in peripheral blood cells count induced by radiotherapy.Conclusion:Combinative therapy can enhance immune functions for S180-bearing mice.
- 0
- |
- 173
- |
- 6
发布时间：2024-09-23 - 摘要：Objective: To investigate the anti-tumor and immune effect of Fuzheng Guben Granule combined with chemotherapy and radiotherapy in tumor-bearing mice. Method: Tumor-bearing model of S180 sarcoma in mice was established by subcutaneous injection of S-180 ascites at armpit. On the next day, the mice were randomly divided into 10 groups, Fuzheng Guben Granule with radio- and chemotherapy was carried out once a day for ten days. Tumor inhibitory percentage, thymus gland,spleen indexes were then measured. The determination of cytokine, such as IL-2,IL-6,TNF-α and peripheral blood cell counts was made. The effect of treatment on tumor inhibition and immunological function was studied. Result: The combined therapy could significantly increase thymus gland,spleen indexes and improved the phagocytic function;antagonize serum IL-2, IL-6 and TNF-α reduction induced by chemotherapy;suppress the decrease in peripheral blood cells count induced by radiotherapy. Conclusion: Combinative therapy can enhance immune functions for S180-bearing mice.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the effect of Shutong Capsule on defecating function with type of dry stagnation in stomach and intestines.Method: Through feeding with rice and prohibition of drinking water three days,we prepared the rat model and mouse model with coprostasis type of dry stagnation in stomach and intestines.And then,using Shutong Capsule（high,medium and low dosage,ig） as the treatment,first time of defecation and grains of defecation in 4 h for normal mice and dry stool constipation mice,and periotalsis of intestine for normal rats and dry stool constipation rats were all observed and recorded.Result:There were no impact on purgation for normal mice and rat.There were significant differences between all the drug groups and model group in first time of defecation and grains of defecation in 4 h of mice（P < 0.01 or P < 0.05）.The effect of Shutong high,medium dosage group was more evident than that in the low one（P < 0.001）.Shutong high,medium dosage group were significantly increased comparing with model group in the pushing length and percentage of rat intestine（P < 0.001 or P < 0.01）.Conclusion:Shutong Capsule can moisten intestines and urge purgation,increase the peristalic rate of intestine for type of dry stagnation in stomach and intestines.
- 0
- |
- 177
- |
- 20
发布时间：2024-09-23 - 摘要：Objective: To study the effect of Shutong Capsule on defecating function with type of dry stagnation in stomach and intestines. Method: Through feeding with rice and prohibition of drinking water three days,we prepared the rat model and mouse model with coprostasis type of dry stagnation in stomach and intestines. And then,using Shutong Capsule (high,medium and low dosage, ig) as the treatment, first time of defecation and grains of defecation in 4h for normal mice and dry stool constipation mice, and periotalsis of intestine for normal rats and dry stool constipation rats were all observed and recorded. Result: There were no impact on purgation for normal mice and rat.There were significant differences between all the drug groups and model group in first time of defecation and grains of defecation in 4h of mice ( P ＜0.01 or P ＜0.05).The effect of Shutong high,medium dosage group was more evident than that in the low one ( P ＜0.001). Shutong high,medium dosage group were significantly increased comparing with model group in the pushing length and percentage of rat intestine ( P ＜0.001 or P ＜0.01). Conclusion: Shutong Capsule can moisten intestines and urge purgation,increase the peristalic rate of intestine for type of dry stagnation in stomach and intestines.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the influence of turtles and deer antlers granula on learning and memory in the natural aging mice.Method:The natural aging of 14 months mice were randomly divided into model control group,administration control group and the positive control group.Drugs was prepared in drinking water and administrated to the rats for consecutive 3 months.In addition,2 month-old mice were used as the young control group.Wrong and escape response latencies with step-down test for each group was investigated 4 days before the last administration.Two days before the last administration the error response latency and error response times within 5 min was detected by avoiding dark method.After the treatment of drugs,blood serum and brain homogenate were separated and prepared,superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） activity and malondialdehyde（MDA） content was detected.Result:Compared with young control group error response latency of aged model group mice was significantly shorter,escape response latency was significantly longer,the error reaction times increased significantly,SOD,GSH-Px activity significantly decreased,MDA content increased significantly.Compared with the aging model group,error response latency of the administration control group aged mice was significantly prolonged,escape response latency was significantly shortened,error response rate was significantly decreased,SOD,GSH-Px activities were significantly increased,MDA content was significantly lower.Conclusion:The turtle and deer antlers granula can enhance antioxidant and improve learning and memory for the natural aging mice.关键词：turtle and deer antlers granula;natural aging;learning and memory;antioxidant
- 0
- |
- 164
- |
- 5
发布时间：2024-09-23 - 摘要：Objective: To study the influence of turtles and deer antlers granula on learning and memory in the natural aging mice. Method: The natural aging of 14 months mice were randomly divided into model control group, administration control group and the positive control group. Drugs was prepared in drinking water and administrated to the rats for consecutive 3 months. In addition, 2 month-old mice were used as the young control group. Wrong and escape response latencies with step-down test for each group was investigated 4 days before the last administration. Two days before the last administration the error response latency and error response times within 5 min was detected by avoiding dark method. After the treatment of drugs, blood serum and brain homogenate were separated and prepared, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA ) content was detected. Result: Compared with young control group error response latency of aged model group mice was significantly shorter, escape response latency was significantly longer, the error reaction times increased significantly, SOD, GSH-Px activity significantly decreased, MDA content increased significantly. Compared with the aging model group, error response latency of the administration control group aged mice was significantly prolonged, escape response latency was significantly shortened, error response rate was significantly decreased, SOD, GSH-Px activities were significantly increased, MDA content was significantly lower. Conclusion: The turtle and deer antlers granula can enhance antioxidant and improve learning and memory for the natural aging mice.关键词：turtle and deer antlers granula;natural aging;learning and memory;antioxidant
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To study the effects of Ginkgo biloba extract on renal hypertension rats (RHR). The serum nitric oxide (NO) content and the content of superoxide dismutase (SOD), maleic disldehyde (MDA) in heart, brain and kidney tissue were investigated. Method: With the method of 2K1C, RHR were established and then the RHR were divided into five groups based on their blood pressure:blank group, model group, G.biloba extract high, middle, low dosage group and captopril group (n=8, each). Systolic blood pressure(SBP) and diastolic blood pressure(DBP) were measured respectively before therapy and two weeks, four weeks, six weeks, eight weeks after therapy. The serum content of NO and MDA,SOD in heart, brain and kidney were measured after eight weeks of therapy. Result: After eight weeks treatment, G.biloba extract significantly declined the pressure of RHR. captopril group and G.biloba extract high group had no significant difference. The two groups both increased the serum of NO and SOD in heart, brain and kidney, declined the MDA. Conclusion: The anti-hypertensive function of G.biloba extract may be related to NO, and protect target organ via antioxidation.关键词：ginkgo biloba extract;nitric oxid;renal hypertension rats;superoxide dismutase;malondialdehyde
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the effects of Ginkgo biloba extract on renal hypertension rats（RHR）.The serum nitric oxide（NO） content and the content of superoxide dismutase（SOD）,maleic disldehyde（MDA） in heart,brain and kidney tissue were investigated.Method:With the method of 2K1C,RHR were established and then the RHR were divided into five groups based on their blood pressure:blank group,model group,G.biloba extract high,middle,low dosage group and captopril group（n = 8,each）.Systolic blood pressure（SBP） and diastolic blood pressure（DBP） were measured respectively before therapy and two weeks,four weeks,six weeks,eight weeks after therapy.The serum content of NO and MDA,SOD in heart,brain and kidney were measured after eight weeks of therapy.Result:After eight weeks treatment,G.biloba extract significantly declined the pressure of RHR.captopril group and G.biloba extract high group had no significant difference.The two groups both increased the serum of NO and SOD in heart,brain and kidney,declined the MDA.Conclusion: The anti-hypertensive function of G.biloba extract may be related to NO,and protect target organ via antioxidation.关键词：ginkgo biloba extract;nitric oxid;renal hypertension rats;superoxide dismutase;malondialdehyde
- 0
- |
- 287
- |
- 18
发布时间：2024-09-23 - 摘要：目的:研究在抗炎、镇痛、活血化瘀3方面新工艺冷冻干燥法与旧工艺沸腾干燥法制成的2种新伤去瘀颗粒的药效学作用的差异。方法:将新工艺新伤去瘀冻干颗粒高、中、低3个剂量组,与旧工艺组、阳性对照组进行比较,采用蛋清致大鼠足趾肿胀法及二甲苯致小鼠耳廓肿胀法评价抗炎作用;采用热板法和醋酸扭体法观察镇痛作用;采用激光多普勒血流仪测定小鼠耳廓微循环血流量以评估其活血化瘀能力。结果:新伤去瘀冻干颗粒能显著抑制大鼠足趾和小鼠耳廓肿胀等急性炎症;能提高小鼠热板法的痛阈值和拮抗醋酸致小鼠扭体次数的增加;能明显增加小鼠耳廓微循环的血流量。结论:新伤去瘀冻干颗粒具有良好的抗炎、镇痛和活血化瘀作用。关键词：新伤去瘀冻干颗粒;抗炎;镇痛;微循环
- 0
- |
- 59
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the influence of Huanglian Jiedu Decoction on NF-κB,TNF-α of liver in diabetic rats,explore therapeutic mechanism of Huanglian Jiedu Decoction on diabetes.Method: The rat diabetic model was made by high fat diet and small doze of streptozotocin（STZ）.After giving Huanglian Jiedu Decoction（11.5,5.7,2.85 g.kg-1）for 8 weeks,FGB,CHO,TG,LDLC and HDLC were measure,the liver and pancreas mass indexes were calulated.Using the immunohistochemical methods the contents of NF-κB and TNF-α in the liver of rats were determined.Result: High-dosage group and middle-dosage group of Huanglian Jiedu Decoction could significantly decrease the contents of CHO,TG,LDLC in rat serum,increase the contents of HDLC,lower the liver and pancreas mass indexes obviously.The expression of NF-κB,TNF-α in the liver was lower in the treatment groups compared with that in the model group.Conclusion: Huanglian Jiedu Decoction has therapeutic effect on diabetic rats induced by high fat diet and small doze of streptozotocin.The mechanism might be related to regulating the expression of NF-κB,TNF-α,inhibiting inflammatory signal pathway.关键词：Huanglian Jiedu Decoction;diabetes;NF-κB;TNF-α
- 0
- |
- 409
- |
- 33
发布时间：2024-09-23 - 摘要：Objective: To study the influence of Huanglian Jiedu Decoction on NF-κB, TNF-α of liver in diabetic rats, explore therapeutic mechanism of Huanglian Jiedu Decoction on diabetes. Method: The rat diabetic model was made by high fat diet and small doze of streptozotocin (STZ).After giving Huanglian Jiedu Decoction (11.5, 5.7, 2.85 g ·kg-1)for 8 weeks, FGB, CHO, TG, LDLC and HDLC were measure, the liver and pancreas mass indexes were calulated.Using the immunohistochemical methods the contents of NF-κB and TNF-α in the liver of rats were determined. Result: High-dosage group and middle-dosage group of Huanglian Jiedu Decoction could significantly decrease the contents of CHO, TG, LDLC in rat serum, increase the contents of HDLC, lower the liver and pancreas mass indexes obviously.The expression of NF-κB, TNF-α in the liver was lower in the treatment groups compared with that in the model group. Conclusion: Huanglian Jiedu Decoction has therapeutic effect on diabetic rats induced by high fat diet and small doze of streptozotocin. The mechanism might be related to regulating the expression of NF-κB, TNF-α, inhibiting inflammatory signal pathway.关键词：Huanglian Jiedu Decoction;diabetes;NF-κB;TNF-α
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:研究培养雪莲总黄酮对炎性介质一氧化氮（NO）,前列腺素E2（PGE2）分泌以及一氧化氮合酶（iNOS）,环氧酶-2（COX-2）mRNA表达的影响,探讨其抗炎机制。方法:用Griess法测定培养雪莲总黄酮对Raw264.7细胞NO的影响,放射免疫法测定培养雪莲总黄酮对Raw264.7细胞PGE2含量;逆转录聚合酶链式反应方法检测Raw264.7细胞iNOS,COX-2mRNA表达情况。结果:培养雪莲总黄酮可明显降低Raw264.7细胞上清中NO,PGE2含量,同时,降低iNOS,COX-2 mRNA表达。结论:培养雪莲总黄酮的抗炎作用与抑制炎性介质NO,PGE2分泌有关,抑制iNOS,COX-2 mRNA表达从而降低炎性介质NO,PGE2分泌可能是培养雪莲总黄酮的抗炎作用的机制之一。关键词：雪莲总黄酮;一氧化氮;前列腺素E2;一氧化氮合酶;环氧酶-2
- 0
- |
- 220
- |
- 9
发布时间：2024-09-23 - 摘要：Objective: To investigate the factors of passive cutaneous anaphylaxis(PCA) in rats to select experimental conditions and improve the accuracy of forecasts for experiments. Method: Egg albumin and bovine serum albumin were selected as test antigens for the PCA. PCA experiments were carried out in different experimental conditions (with or without kasa agents, allergens means, rat strains, the optimal time for preparing antiserum of blood withdrawn, the difference in immunogenicity of egg albumin and bovine serum albumin,etc.). Result: ① Adjuvant could significantly enhance the sensitivity of immune response. in SD and Wistar tast.② Intraperitoneally and subcutaneously injection sensitization both could get good response of PCA, but intraperitoneally injection with adjuvant could induce chronic inflammation of abdominal cavity. ③ Sensitivity of immune response to antigen was similar in SD and Wistar rats. However, to use different strains of rats with PCA were better than to use single stain of experimental rats. ④ Content of IgE in serum after 16 days sensitization was higher than that in 14 days sensitization. ⑤ Egg albumin was superior to bovine serum albumin in immunogenicity of PCA test.关键词：passive cutaneous anaphylaxis (PCA);Freund's complete adjuvant;SD rat;Wistar rat
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the factors of passive cutaneous anaphylaxis（PCA） in rats to select experimental conditions and improve the accuracy of forecasts for experiments.Method:Egg albumin and bovine serum albumin were selected as test antigens for the PCA.PCA experiments were carried out in different experimental conditions（with or without kasa agents,allergens means,rat strains,the optimal time for preparing antiserum of blood withdrawn,the difference in immunogenicity of egg albumin and bovine serum albumin,etc.）.Result:① Adjuvant could significantly enhance the sensitivity of immune response.in SD and Wistar tast.② Intraperitoneally and subcutaneously injection sensitization both could get good response of PCA,but intraperitoneally injection with adjuvant could induce chronic inflammation of abdominal cavity.③ Sensitivity of immune response to antigen was similar in SD and Wistar rats.However,to use different strains of rats with PCA were better than to use single stain of experimental rats.④ Content of IgE in serum after 16 days sensitization was higher than that in 14 days sensitization.⑤ Egg albumin was superior to bovine serum albumin in immunogenicity of PCA test.关键词：passive cutaneous anaphylaxis（PCA）;Freund’s complete adjuvant;SD rat;Wistar rat
- 0
- |
- 405
- |
- 14
发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of Icariin (ICA) on intimal proliferation and apoptosis of vascular smooth muscle cell after vascular injury in rat. Method : Fourty-four rats were randomly divided into 4 groups: natural group,model group and ICA 60,120 mg ·kg-1 group.Rat model of carotid restenosis induced by balloon injury was established. Rats of ICA group were treated with drugs for 2 or 4 weeks. Then the neointimal area(IA),medial area (MA), and neointimal area(IA)/medial area(MA) were analyzed by computerized digitizer system. TUNEL method was used to detected the apoptosis in the artery. Result :At each time point, the lumem area was larger and the neointima area was smaller in all the treated groups compared with that in model group (P<0.01). The expression of vascular smooth muscle cell apoptosis in the vascular in all the treated groups was increased, that of the model group (2 w, 4 w) being the lowest (P <0.01). Conclusion : ICA can effectively repress neointimal proliferation after vascular injury, The effect may be associated with its action of promoting apoptosis of vascular smooth muscle cells.关键词：icariin;neointima;vascular smooth muscle cell;apoptosis
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Icariin（ICA） on intimal proliferation and apoptosis of vascular smooth muscle cell after vascular injury in rat.Method: Fourty-four rats were randomly divided into 4 groups: natural group,model group and ICA 60,120 mg.kg-1 group.Rat model of carotid restenosis induced by balloon injury was established.Rats of ICA group were treated with drugs for 2 or 4 weeks.Then the neointimal area（IA）,medial area（MA）,and neointimal area（IA） /medial area（MA） were analyzed by computerized digitizer system.TUNEL method was used to detected the apoptosis in the artery.Result :At each time point,the lumem area was larger and the neointima area was smaller in all the treated groups compared with that in model group（P < 0.01）.The expression of vascular smooth muscle cell apoptosis in the vascular in all the treated groups was increased,that of the model group（2 w,4 w） being the lowest（P < 0.01）.Conclusion: ICA can effectively repress neointimal proliferation after vascular injury,The effect may be associated with its action of promoting apoptosis of vascular smooth muscle cells.关键词：icariin;neointima;vascular smooth muscle cell;apoptosis
- 0
- |
- 172
- |
- 7
发布时间：2024-09-23 - 摘要：Objective: To observe the effects of Quzhi Baogan Granules on the rat’s fatty liver. Method: The rat’s fatty liver was induced by high fat diet. The different groups were given different drugs, to observe the efficacy of Quzhi Baogan Granules on the rat’s fatty liver,meanwhile to investigate the mechanism of the granules on the rat’s fatty liver. Result: Compared with blank control group, liver tissue CHO, TG levels in the models were increased significantly(P<0.01), serum AST, ALT were significantly increased(P<0.01). Pathological investigation showed that liver cell steatosis was took place associated with cell injury and necrosis. Compared with model group,the liver tissue CHO, TG levels in the Quzhi Baogan high-dose group,mid-dose group,low-dose group were decreased obviously( P <0.01 or P <0.05),the serum AST, ALT were decreased obviously( P <0.01 or P <0.05), meanwhile the liver injury were alleviated. Conclusion: Quzhi Baogan Granules can reduce liver cell injury through the antioxidant mechanism,it can prevent and treat the rat’s fatty liver induced by high fat diet.关键词：Quzhi Baogan Granules;high lipid diet;Fatty liver;antioxidation
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Quzhi Baogan Granules on the rat’s fatty liver.Method:The rat’s fatty liver was induced by high fat diet.The different groups were given different drugs,to observe the efficacy of Quzhi Baogan Granules on the rat’s fatty liver,meanwhile to investigate the mechanism of the granules on the rat’ s fatty liver.Result:Compared with blank control group,liver tissue CHO,TG levels in the models were increased significantly（P < 0.01）,serum AST,ALT were significantly increased（P < 0.01）.Pathological investigation showed that liver cell steatosis was took place associated with cell injury and necrosis.Compared with model group,the liver tissue CHO,TG levels in the Quzhi Baogan high-dose group,mid-dose group,low-dose group were decreased obviously（P < 0.01 or P < 0.05）,the serum AST,ALT were decreased obviously（P < 0.01 or P < 0.05）,meanwhile the liver injury were alleviated.Conclusion:Quzhi Baogan Granules can reduce liver cell injury through the antioxidant mechanism,it can prevent and treat the rat’s fatty liver induced by high fat diet.关键词：Quzhi Baogan Granules;high lipid diet;Fatty liver;antioxidation
- 0
- |
- 82
- |
- 2
发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of wogonin on the proliferation and cell cycle of the SPC-A-1 cell line. Method :The cultured SPC-A-1 cells were treated with different concentrations of wogonin. The proliferation of SPC-A-1 cells was detected by MTT method. Cell apoptosis and cell cycle were detected by AO+EB straining and flow cytometry. Result :After wogonin administration, SPC-A-1 cells proliferation inhibition ratio and apoptosis ratio increased significantly in a dose-dependent manner. AO+EB straining showed partial cells presented characteristic morphological changes of apoptosis.SPC-A-1 cells showed G0/G1 phase blockage when the concentration of wogonin was 10,20 mg ·L-1, and S phase blockage appeared when the concentration of wogonin was 30, 40 mg ·L-1. Conclusion :Wogonin can inhibit the proliferation of SPC-A-1 cells and induce apoptosis, also possess the effect of G0/G1 and S phase blockage and potential antitumor effect.关键词：wogonin;SPC-A-1 cells;proliferation;cell cycle
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of wogonin on the proliferation and cell cycle of the SPC-A-1 cell line.Method: The cultured SPC-A-1 cells were treated with different concentrations of wogonin.The proliferation of SPC-A-1 cells was detected by MTT method.Cell apoptosis and cell cycle were detected by AO + EB straining and flow cytometry.Result:After wogonin administration,SPC-A-1 cells proliferation inhibition ratio and apoptosis ratio increased significantly in a dose-dependent manner.AO + EB straining showed partial cells presented characteristic morphological changes of apoptosis.SPC-A-1 cells showed G0 /G1 phase blockage when the concentration of wogonin was 10,20 mg.L-1,and S phase blockage appeared when the concentration of wogonin was 30,40 mg.L-1.Conclusion:Wogonin can inhibit the proliferation of SPC-A-1 cells and induce apoptosis,also possess the effect of G0 /G1 and S phase blockage and potential antitumor effect.关键词：wogonin;SPC-A-1 cells;proliferation;cell cycle
- 0
- |
- 303
- |
- 17
发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Bitter Apricot Seed,Platycodon Root,and their compatibility for relieving cough and expelling phlegm.Method:Cough model was made by ammonia stimulation.Cough latency and cough times of the mice were recorded respectively.A mouse model of phenol red excretion was used to evalute the phlegm expelling.Result: Bitter Apricot Seed,Platycodon Root,and their compatibility all significantly reduced the cough times in the mice.Furthermore,the cough times of the compatibility group were significantly less than that of bitter apricot seed group and platycodon root group in the same dose.The phenol red excretion quantity of all groups of Bitter Apricot Seed,Platycodon Root,and their compatibility were significantly increased than that of control group.And there were no significant differences between the groups of Bitter Apricot Seed,Platycodon Root,and their compatibility.Conclusion:Both Bitter Apricot Seed,Platycodon Root and their compatibility have the evident effect of relieving cough and expelling phlegm.Furthermore,the compatibility of Bitter Apricot Seed and Platycodon Root increased the efficacy of relieving cough.However,the efficacy for expelling phlegm did not increased by their compatibility in the experiment.Whether the compatibility of Bitter Apricot Seed and Platycodon Root can increase the efficacy of expelling phlegm should be answered in further studies.关键词：Bitter Apricot Seed;Platycodon Root;compatibility;anti-cough;removing the phlegm
- 0
- |
- 1049
- |
- 63
发布时间：2024-09-23 - 摘要：Objective: To observe the effect of Bitter Apricot Seed, Platycodon Root, and their compatibility for relieving cough and expelling phlegm. Method: Cough model was made by ammonia stimulation. Cough latency and cough times of the mice were recorded respectively. A mouse model of phenol red excretion was used to evalute the phlegm expelling. Result: Bitter Apricot Seed, Platycodon Root, and their compatibility all significantly reduced the cough times in the mice. Furthermore, the cough times of the compatibility group were significantly less than that of bitter apricot seed group and platycodon root group in the same dose. The phenol red excretion quantity of all groups of Bitter Apricot Seed, Platycodon Root, and their compatibility were significantly increased than that of control group. And there were no significant differences between the groups of Bitter Apricot Seed, Platycodon Root, and their compatibility. Conclusion: Both Bitter Apricot Seed, Platycodon Root and their compatibility have the evident effect of relieving cough and expelling phlegm. Furthermore, the compatibility of Bitter Apricot Seed and Platycodon Root increased the efficacy of relieving cough. However, the efficacy for expelling phlegm did not increased by their compatibility in the experiment. Whether the compatibility of Bitter Apricot Seed and Platycodon Root can increase the efficacy of expelling phlegm should be answered in further studies.关键词：Bitter Apricot Seed;Platycodon Root;compatibility;anti-cough;removing the phlegm
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To copy the rabbit model of necrosis of the femoral head,observe the number of osteoclasts,study the feasibility and mechanism of intramedullary injection with Salvia miltiorrhiza injection for preventing of steroid-induced necrosis of the femoral head at the molecular level.Method: Thirty rabbits were randomly divided into three groups: control group,model group,prevention group,10 in each group.Glucocorticoid-induced rabbit model of femoral head necrosis was prepared.While making the models,the prevention group has received bone marrow cavity intramedullary injection with Salvia miltiorrhiza.at a dose of 0.4 mg.kg-1 twice a week for 8 weeks.8 weeks later,the number of osteoclasts and histopathological changes in three groups of rabbits were observed.Result: The prevention group could effectively reduce the number of osteoclasts and the rate of empty lacunae femoral head.Conclusion: Salvia miltiorrhiza injection plays a good effective role in the prevention of steroid-induced avascular necrosis of femoral head through inhibitting osteoclast formation.关键词：avascular necrosis of femoral head;glucocorticoid;intramedullary injection;Salvia miltiorrhiza;osteoclast
- 0
- |
- 196
- |
- 18
发布时间：2024-09-23 - 摘要：Objective: To copy the rabbit model of necrosis of the femoral head,observe the number of osteoclasts, study the feasibility and mechanism of intramedullary injection with Salvia miltiorrhiza injection for preventing of steroid-induced necrosis of the femoral head at the molecular level. Method: Thirty rabbits were randomly divided into three groups: control group, model group, prevention group, 10 in each group. Glucocorticoid-induced rabbit model of femoral head necrosis was prepared. While making the models,the prevention group has received bone marrow cavity intramedullary injection with Salvia miltiorrhiza. at a dose of 0.4 mg ·kg-1 twice a week for 8 weeks. 8 weeks later, the number of osteoclasts and histopathological changes in three groups of rabbits were observed. Result : The prevention group could effectively reduce the number of osteoclasts and the rate of empty lacunae femoral head. Conclusion : Salvia miltiorrhiza injection plays a good effective role in the prevention of steroid-induced avascular necrosis of femoral head through inhibitting osteoclast formation.关键词：avascular necrosis of femoral head;glucocorticoid;intramedullary injection;Salvia miltiorrhiza;osteoclast
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To validate the scientific rationality of optimized extraction technics for Banxia Baizhu Tianma decoction in SBEE experiment with main efficacy as indicators. Method :Rat model of hypertension with abundant phlegm-dampness was estabilished. Eleven indicators closely associated with abundant phlegm-dampness were selcted for evaluating effects. indicators closely associated with abundant phlegm-damphess.Four kinds of solution of Banxia Baizhu Tianma decoction (SBEE,SBE,WE,AE)were compared for their effectiveness. Result :Effects on the hypertensive rat showed that SBEE solution had the best effect. Conclusion :SBEE is the best extracting method for Banxia Baizhu Tianma decoction.关键词：Banxia Baizhu Tianma decoction;semi-bionic enzyme extraction;hypertension with abundant phlegm-dampness
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To validate the scientific rationality of optimized extraction technics for Banxia Baizhu Tianma decoction in SBEE experiment with main efficacy as indicators.Method:Rat model of hypertension with abundant phlegm-dampness was estabilished.Eleven indicators closely associated with abundant phlegm-dampness were selcted for evaluating effects.indicators closely associated with abundant phlegm-damphess.Four kinds of solution of Banxia Baizhu Tianma decoction（SBEE,SBE,WE,AE） were compared for their effectiveness.Result: Effects on the hypertensive rat showed that SBEE solution had the best effect.Conclusion: SBEE is the best extracting method for Banxia Baizhu Tianma decoction.关键词：Banxia Baizhu Tianma decoction;semi-bionic enzyme extraction;hypertension with abundant phlegm-dampness
- 0
- |
- 322
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: To prepare the total glycosides of Paeonia lactiflora, and investigate its protection towards carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice. Method : Macroporous adsorption chromatography was used to enrich its total glycosides. Carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice were utilized to investigate the effect of total glycosides of P. lactiflora on serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities. Meanwhile, its effect on total cholesterol (TC) and triglyceride (TG) contents in liver was studied in DL-ethionine induced acute fatty liver model. Result : The polysaccharide and some other components was eluted off from the column of AB-8 resins with 10% ethanol, afterwards the remaining total glycosides was eluted off from the column by washing continuously with 50% ethanol, and its content was above 50%. On carbon tetrachloride induced acute liver injury model, the total glycosides could significantly reduce the increase of serum ALT and AST activities induced by CCl4; on DL-ethionine induced acute fatty liver model, there was no significant difference of serum ALT and AST activities between the model group and the control group, however, the content of total cholesterol and triglyceride in the liver of model groups significantly increased and decreased after treatment with total glycosides. Conclusion : The total glycosides of P. lactiflora has a protective effect on CCl4 induced acute liver injury and DL-ethionine induced acute fatty liver in mice.关键词：total glycosides of Paeonia lactiflora.;Macroporous resin;liver injury;DL-ethionine
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To prepare the total glycosides of Paeonia lactiflora,and investigate its protection towards carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice.Method: Macroporous adsorption chromatography was used to enrich its total glycosides.Carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice were utilized to investigate the effect of total glycosides of P.lactiflora on serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities.Meanwhile,its effect on total cholesterol（TC） and triglyceride（TG） contents in liver was studied in DL-ethionine induced acute fatty liver model.Result: The polysaccharide and some other components was eluted off from the column of AB-8 resins with 10% ethanol,afterwards the remaining total glycosides was eluted off from the column by washing continuously with 50% ethanol,and its content was above 50%.On carbon tetrachloride induced acute liver injury model,the total glycosides could significantly reduce the increase of serum ALT and AST activities induced by CCl4;on DL-ethionine induced acute fatty liver model,there was no significant difference of serum ALT and AST activities between the model group and the control group,however,the content of total cholesterol and triglyceride in the liver of model groups significantly increased and decreased after treatment with total glycosides.Conclusion: The total glycosides of P.lactiflora has a protective effect on CCl4 induced acute liver injury and DL-ethionine induced acute fatty liver in mice.关键词：total glycosides of Paeonia lactiflora.;Macroporous resin;liver injury;DL-ethionine
- 0
- |
- 582
- |
- 33
发布时间：2024-09-23 - 摘要：Objective: To study the effect of Huoxue Lishi prescription(HLP) on rats renal injury induced by alcohol. Method : The animal model of alcoholic renal injury was established with the method of direct intragastric infusion of ethanol and HLP. At the end of 4,8,12 weeks, the blood ureanitrogen(BUN), and creatinine(Scr) were measured and the renal pathological changes were observed by HE staining and transmission electron microscope,respectively. Result : In HLP group, BUN and Cr were all decreased (P<0.05,P<0.01) and some pathological changes,such as hyperplasia of mesangium,interstitial infiltration of inflammatory cell,the degeneration of renal tubularepithelial cell and the dilatation of tubule appeared late and were attenuated as time progressed, compared with that of alcohol group(P<0.05). Conclusion : Huoxue Lishi prescription could inhibit rats renal injury induced by alcohol.关键词：alcoholic kidney damage;traditional Chinese medicine;Huoxue Lishi prescription
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the effect of Huoxue Lishi prescription（HLP） on rats renal injury induced by alcohol.Method: The animal model of alcoholic renal injury was established with the method of direct intragastric infusion of ethanol and HLP.At the end of 4,8,12 weeks,the blood ureanitrogen（BUN）,and creatinine（Scr） were measured and the renal pathological changes were observed by HE staining and transmission electron microscope,respectively.Result: In HLP group,BUN and Cr were all decreased（P < 0.05,P < 0.01） and some pathological changes,such as hyperplasia of mesangium,interstitial infiltration of inflammatory cell,the degeneration of renal tubularepithelial cell and the dilatation of tubule appeared late and were attenuated as time progressed,compared with that of alcohol group（P < 0.05）.Conclusion: Huoxue Lishi prescription could inhibit rats renal injury induced by alcohol.关键词：alcoholic kidney damage;traditional Chinese medicine;Huoxue Lishi prescription
- 0
- |
- 119
- |
- 10
发布时间：2024-09-23 - 摘要：Objective:To detect the hepatic toxicity after giving large dose of Radix et Rhizoma Sophorae Tonkinensis（RRST） to rats for a long time.Method: The rats were divided into 3 groups（control group,12 g.kg-1 drug group,20 g.kg-1 drug group） and given the drug for 26 days.During the days,the rats’behave and weights were observed.After giving the drug for the last time,the rats were anaesthetized by 25% Urethane and the rats’blood was extracted from aorta abdominalis,then liver function was detected by using the blood serum.The rats’livers were taken and weighed,after that,the viscera index was calculated.According to the demand of electron microscope,hepatic tissues were fixed and ultrastructures were detected.Biochemical indicators such as ALT,AST,ALP,TBiL,TP,GLO and ALB were detected by using automatic analyzer.Result: After giving RRST for 10 days,the activity of the rats in drug group decreased,what’s more,the rats had general fatigue and the amount their excrement and urine increased.After giving drug for 26 days,compared with normal group,the ALT and viscera index increased in drug group（P < 0.01 or P < 0.05）,the TP,ALB decreased in drug group（P < 0.01 or P < 0.05）;Through the electron microscope,the results showed that in RRST group,the shape of nucleus changed,nucleus had the tendency of swelling,nuclear membranes shrunk,bile capillaries enlarged and smooth surfaced endoplasmic reticulum enlarged slightly.Conclusion: RRST can cause hepatic toxicity in rats by intragastric administration and the toxicity will increase if the dose of drug is added.关键词：Radix et Rhizoma Sophorae Tonkinensis;rats;hepatic toxicity
- 0
- |
- 493
- |
- 25
发布时间：2024-09-23 - 摘要：Objective: To detect the hepatic toxicity after giving large dose of Radix et Rhizoma Sophorae Tonkinensis(RRST) to rats for a long time. Method : The rats were divided into 3 groups (control group,12 g ·kg-1 drug group, 20 g ·kg-1 drug group) and given the drug for 26 days. During the days, the rats’ behave and weights were observed. After giving the drug for the last time, the rats were anaesthetized by 25% Urethane and the rats’ blood was extracted from aorta abdominalis, then liver function was detected by using the blood serum. The rats’ livers were taken and weighed, after that, the viscera index was calculated. According to the demand of electron microscope, hepatic tissues were fixed and ultrastructures were detected. Biochemical indicators such as ALT, AST, ALP, TBiL, TP, GLO and ALB were detected by using automatic analyzer. Result : After giving RRST for 10 days, the activity of the rats in drug group decreased, what’s more, the rats had general fatigue and the amount their excrement and urine increased. After giving drug for 26 days, compared with normal group, the ALT and viscera index increased in drug group (P<0.01 or P<0.05),the TP, ALB decreased in drug group (P<0.01 or P<0.05); Through the electron microscope, the results showed that in RRST group,the shape of nucleus changed, nucleus had the tendency of swelling, nuclear membranes shrunk, bile capillaries enlarged and smooth surfaced endoplasmic reticulum enlarged slightly. Conclusion : RRST can cause hepatic toxicity in rats by intragastric administration and the toxicity will increase if the dose of drug is added.关键词：Radix et Rhizoma Sophorae Tonkinensis;rats;hepatic toxicity
- 2
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To observe IL-8 and TNF-α mRNA expression of rabbit sinus mucosa of Biyuanshu treatment of chronic rhinosinusitis（CRS） model,and explore the possible mechanism of Biyuanshu treatment of CRS.Method: Sixty new Zealand rabbits fed one week after adaptation and were randomLy divided into normal group,model group,sham operation group,Biyuanshu group,western medicine group,12 in each group.Firstly CRS model have established,then Biyuanshu group and western medicine group were given 1.5 mL.kg-1 of BiYuanShu or 25 mg.kg-1of clarithromycin every day in14 days,finally they were sacrificed to take sinus mucosa,HE staining were observed by light microscopy and IL-8 and TNF-α mRNA expression of sinus mucosa were detected by real-time quantitative PCR.Result: In model group,the sinus mucosa showed chronic inflammation and mucosal infiltration of inflammatory cells,and epithelial cells and glands and hyperplasia of goblet cells were found,IL-8 and TNF-α mRNA expression of sinus mucosal were higher than normal group significantly（P < 0.01）.In Biyuanshu group,sinus mucosa have good repair,and inflammatory cells and glands and goblet cell hyperplasia were not obvious,IL-8 and TNF-α mRNA expression of sinus mucosal were lower than model groupsignificantly（P < 0.01）,Biyuanshu group and normal group have no significant difference.Conclusion: The mechanism of Biyuanshu treatment of CRS may be related to regulation of IL-8 and TNF-α mRNA expression of sinus mucosal.关键词：Biyuanshu;CRS;IL-8;TNF-α;mRNA
- 0
- |
- 169
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: To observe IL-8 and TNF-α mRNA expression of rabbit sinus mucosa of Biyuanshu treatment of chronic rhinosinusitis (CRS) model, and explore the possible mechanism of Biyuanshu treatment of CRS. Method : Sixty new Zealand rabbits fed one week after adaptation and were randomLy divided into normal group, model group, sham operation group, Biyuanshu group, western medicine group, 12 in each group. Firstly CRS model have established, then Biyuanshu group and western medicine group were given 1.5 mL ·kg-1of BiYuanShu or 25 mg ·kg-1of clarithromycin every day in14 days, finally they were sacrificed to take sinus mucosa, HE staining were observed by light microscopy and IL-8 and TNF-α mRNA expression of sinus mucosa were detected by real-time quantitative PCR. Result : In model group, the sinus mucosa showed chronic inflammation and mucosal infiltration of inflammatory cells, and epithelial cells and glands and hyperplasia of goblet cells were found, IL-8 and TNF-α mRNA expression of sinus mucosal were higher than normal group significantly (P <0.01). In Biyuanshu group, sinus mucosa have good repair, and inflammatory cells and glands and goblet cell hyperplasia were not obvious, IL-8 and TNF-α mRNA expression of sinus mucosal were lower than model group significantly (P <0.01), Biyuanshu group and normal group have no significant difference. Conclusion : The mechanism of Biyuanshu treatment of CRS may be related to regulation of IL-8 and TNF-α mRNA expression of sinus mucosal.关键词：Biyuanshu;CRS;IL-8;TNF-α;mRNA
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a method for the microbial limit test of Shenrong Jianyaojiu.Method: The recovery rates of tested strains treated by Shenrong Jianyaojiu were determined by membrane filtration method.The test method of the control bacteria was verified.Result: In the parallel experiment of 3 indendence,5 tested strains recovery rate all more than 70%.This method can be adopted in bacterial strain control of Shenrong Jianyaojiu.Routine test method can be test for control bacterias（Escherichia coli）.Conclusion: It can eliminate the antimicrobial properties,and it can be used for the microbial limit test for Shenrong Jianyaojiu.关键词：Shenrong Jianyaojiu;microbial limit test;validation of the method
- 0
- |
- 102
- |
- 2
发布时间：2024-09-23 - 摘要：Objective: To establish a method for the microbial limit test of Shenrong Jianyaojiu. Method : The recovery rates of tested strains treated by Shenrong Jianyaojiu were determined by membrane filtration method. The test method of the control bacteria was verified. Result : In the parallel experiment of 3 indendence, 5 tested strains recovery rate all more than 70%. This method can be adopted in bacterial strain control of Shenrong Jianyaojiu. Routine test method can be test for control bacterias(Escherichia coli). Conclusion : It can eliminate the antimicrobial properties, and it can be used for the microbial limit test for Shenrong Jianyaojiu.关键词：Shenrong Jianyaojiu;microbial limit test;validation of the method
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To use Cochrane systematic reviews in evaluation of clinical treatment of traditional Chinese medicine for improving the effectiveness of HIV/AIDS and related symptoms. Method : To search Chinese medicine treatment of HIV/AIDS clinical randomized controlled trials or clinical controlled trials literature, and to apply Meta analysis of literature of the results. Result : A total of 13 selected clinical research literatures. With meta analysis showed that the combined effect of the value of OR=4.62,95％CI(3.35,6.36), the total combined effect of Z=9.38,Ρ＜0.01. The difference was statistically significant, suggesting that Chinese medicine treatment for AIDS and related diseases had excellent clinical efficacy compared with the control group. Conclusion : Chinese medicine may improve the treatment of HIV/AIDS and related symptoms and improve quality of life of HIV/AIDS patients. However, the conclusion, is still subject to large samples of high-quality clinical study to be further verification.关键词：traditional Chinese medicine;AIDS;randomized controlled trial;Meta analysis
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术
- 0
- |
- 14
- |
- 0
发布时间：2024-09-23 -
- 摘要：Objective:To use Cochrane systematic reviews in evaluation of clinical treatment of traditional Chinese medicine for improving the effectiveness of HIV /AIDS and related symptoms.Method: To search Chinese medicine treatment of HIV /AIDS clinical randomized controlled trials or clinical controlled trials literature,and to apply Meta analysis of literature of the results.Result: A total of 13 selected clinical research literatures.With meta analysis showed that the combined effect of the value of OR = 4.62,95% CI（3.35,6.36）,the total combined effect of Z = 9.38,Ρ < 0.01.The difference was statistically significant,suggesting that Chinese medicine treatment for AIDS and related diseases had excellent clinical efficacy compared with the control group.Conclusion: Chinese medicine may improve the treatment of HIV /AIDS and related symptoms and improve quality of life of HIV /AIDS patients.However,the conclusion,is still subject to large samples of high-quality clinical study to be further verification.关键词：traditional Chinese medicine;AIDS;randomized controlled trial;Meta analysis
- 0
- |
- 425
- |
- 20
发布时间：2024-09-23 - 摘要：目的:观察地塞米松磷酸钠注射液封闭联合口服口炎清颗粒治疗糜烂型扁平苔藓的临床疗效。方法:将糜烂型扁平苔藓30例随机分成2组,治疗组16例采用地塞米松磷酸钠注射液2 mg与2%盐酸利多卡因注射液1 mL行病损区基底部封闭,1次/周,连用4周,同时口服口炎清颗粒,20 g（即2袋）,2次/d,连用4周;对照组14例采用口服羟氯喹片,100 mg,2次/d,连用4周。结果:治疗后3个月随访疗效,治疗组16例,有效15例,无效1例;对照组14例,有效9例,无效5例;2组有效率之间有明显差异,治疗组优于对照组（P<0.05）。结论:地塞米松磷酸钠注射液封闭联合口服口炎清颗粒治疗糜烂型扁平苔藓可提高临床疗效。关键词：糜烂型扁平苔藓;地塞米松磷酸钠;口炎清颗粒;羟氯喹
- 0
- |
- 141
- |
- 9
发布时间：2024-09-23 - 摘要：Objective :To observe clinical effect of treating severe acute pancreatitis with modified Dachengqi Tang. Method :Thirty-six cases patients with severe acute pancreatitis were randomly divided into two groups. The control group was treated with conventional medical treatment, and the treatment group was treated with modified Dachengqi Tang based on the control group. The clinical effect, related parameters and adverse reaction were observed for control group and treatment group. Result :The total effective rate of the treatment group and the control group was 88.9% and 66.7% respectively, the clinical effect in the treatment group was significantly higher than that of the control group(P<0.05). Combined with control group, serum/uric amylase and reactive protein decreased significantly(P<0.01或P<0.05)after treatment. serum/uric amylase and reactive protein decreased significantly before treatment in this group, especially in the treatment group. There was a significant difference(P<0.01). The time for body temperature returned to normal, abdominal pain relief, serum/uric amylase returned to normal and leukocyte returned to normal have significant differences(P < 0.05). All the times were shorter for the treatment group. Adverse reaction in the treatment group was lower than that of the control group. Conclusion :The modified Dachengqi Tang for treatment of severe acute pancreatitis is better than conventional medical treatment.关键词：severe acute pancreatitis;Dachengqi Tang;western medicine
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:观察康艾注射液联合GP方案治疗局限晚期胰腺癌的疗效。方法:52例晚期胰腺癌患者,随机分为治疗组26例,男16例,女10例;对照组26例,男15例,女11例;对照组于1,8,15 d予以吉西他滨1 000 mg/m2 gtt,于4～6 d DDP30mg/m2（水化）gtt,28 d为1个周期。化疗2个周期;治疗组在对照组用药基础上于1～20 d加用康艾注射液50 mL加入5%葡萄糖500 mL中gtt,1次/d,1～20 d为1个周期,连用2个周期。两组均于治疗前及治疗2个周期后行CT或MRI检查;每周查血常规和生化全项1次。治疗2个周期后评价疗效及毒副反应。结果:治疗组近期疗效有效率和临床受益反应整体改善率明显优于对照组（P<0.05）。治疗组严重不良反应发生率明显低于对照组（P<0.05）。结论:康艾注射液联合GP方案治疗晚期胰腺癌疗效确切,值得推广。关键词：晚期胰腺癌;康艾注射液;化学治疗
- 0
- |
- 103
- |
- 7
发布时间：2024-09-23 - 摘要：Objective: To observe the intervention effects of a traditional medical formula Jiangtangle on patients of impaired glucose tolerance (IGT). Methods: The 124 cases which have been diagnosed impaired glucose tolerance (IGT) were randomly divided into two groups, treatment group including 64 cases and control group including 60 cases. Blood biochemical indicators of treatment group and control group were analyzed before and after treatment using paired t test; Blood biochemical between treatment group and control group indicators were analyzed using independent sample t test; the efficiency between treatment group and control group before and after treatment was compared using the rank sum test. Result: Fasting plasma glucose (FPG), 2-hour postprandial blood glucose, glycosylated hemoglobin and total cholesterol (TC), triglyceride (TG) between treatment group and control group before and after treatment were statistically different (P<0.05), fasting plasma glucose (FPG), 2-hour postprandial blood glucose, glycosylated hemoglobin and total cholesterol (TC), triglyceride (TG) treatment group and control group were statistically different (P<0.05), total effective rate of treatment group was 91%, that of the control group was 55%, the total effective rate between the two groups was also significant difference (P<0.01). Conclusion: Treatment group was superior to the control group, while Hypoglycemic music on patients with impaired glucose tolerance (IGT) has significant improvement.关键词：impaired glucose tolerance (IGT);traditional medical formula jiangtangle;traditional Chinese medicine therapy
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:观察参芪转律胶囊配合西药常规治疗冠心病收缩性心力衰竭合并室性早搏的临床疗效。方法:根据就诊顺序将60例患者随机分为两组,各30例;两组均采用扩张冠脉、抗血小板聚集、降压、利尿及血管紧张素转换酶抑制剂等常规治疗,治疗组在常规治疗基础上加服参芪转律胶囊,对照组在常规治疗基础上加服美托洛尔抗心律失常治疗。两组疗程均为21 d,1疗程后分别观察心功能恢复情况、室早恢复情况、中医证候改善情况及不良反应发生情况。结果:心功能改善有效率:治疗组73.3℅,对照组60℅;室早有效率:治疗组90℅,对照组66.7℅;中医症候有效率:治疗组83.3℅,对照组56.7℅;不良反应发生率治疗组3.33%,对照组26.67%。两组比较,差异有显著性意义（P<0.05）。治疗后两组室性早搏均有不同程度减少,但治疗组室性早搏减少更为显著,与对照组比较差异有显著性意义（P<0.05）。结论:参芪转律胶囊治疗气阴两虚型冠心病收缩性心力衰竭合并室性早搏疗效显著,可改善患者心功能,具有明显抗心律失常作用。关键词：参芪转律胶囊;慢性收缩性心力衰竭;室性早搏
- 0
- |
- 112
- |
- 8
发布时间：2024-09-23 - 摘要：Objective:To observe clinical effect of treating severe acute pancreatitis with modified Dachengqi Tang.Method :Thirty-six cases patients with severe acute pancreatitis were randomly divided into two groups.The control group was treated with conventional medical treatment,and the treatment group was treated with modified Dachengqi Tang based on the control group.The clinical effect,related parameters and adverse reaction were observed for control group and treatment group.Result:The total effective rate of the treatment group and the control group was 88.9% and 66.7% respectively,the clinical effect in the treatment group was significantly higher than that of the control group（P < 0.05）.Combined with control group,serum /uric amylase and reactive protein decreased significantly（P < 0.01 或 P < 0.05） after treatment.serum /uric amylase and reactive protein decreased significantly before treatment in this group,especially in the treatment group.There was a significant difference（P < 0.01）.The time for body temperature returned to normal,abdominal pain relief,serum /uric amylase returned to normal and leukocyte returned to normal have significant differences（P < 0.05）.All the times were shorter for the treatment group.Adverse reaction in the treatment group was lower than that of the control group.Conclusion:The modified Dachengqi Tang for treatment of severe acute pancreatitis is better than conventional medical treatment.关键词：severe acute pancreatitis;Dachengqi Tang;western medicine
- 0
- |
- 120
- |
- 11
发布时间：2024-09-23 - 摘要：For further detailed the medicinal and efficacy of Lonicerae japonicae flos, the past dynasties ancient books and authoritative pharmic monograph were collected, sorted, compared and analysised, and combined with the modern study on Lonicerae japonicae flos. herbal medicinal textual research on character of medicine and efficacy of Lonicerae japonicae flos. The results showed that the medicinal and efficacy materia medica records were not the same in past dynasties of honeysuckle, and summarized mainstream view that the character of Lonicerae japonicae flos was cold, and the efficacy was clearing heat and detoxicating.关键词：Lonicerae Japonicae Flos;textual;character of medicine
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the intervention effects of a traditional medical formula Jiangtangle on patients of impaired glucose tolerance（IGT）.Methods:The 124 cases which have been diagnosed impaired glucose tolerance（IGT） were randomly divided into two groups,treatment group including 64 cases and control group including 60 cases.Blood biochemical indicators of treatment group and control group were analyzed before and after treatment using paired t test;Blood biochemical between treatment group and control group indicators were analyzed using independent sample t test;the efficiency between treatment group and control group before and after treatment was compared using the rank sum test.Result: Fasting plasma glucose（FPG）,2-hour postprandial blood glucose,glycosylated hemoglobin and total cholesterol（TC）,triglyceride（TG） between treatment group and control group before and after treatment were statistically different（P < 0.05）,fasting plasma glucose（FPG）,2-hour postprandial blood glucose,glycosylated hemoglobin and total cholesterol（TC）,triglyceride（TG） treatment group and control group were statistically different（P < 0.05）,total effective rate of treatment group was 91%,that of the control group was 55%,the total effective rate between the two groups was also significant difference（P < 0.01）.Conclusion: Treatment group was superior to the control group,while Hypoglycemic music on patients with impaired glucose tolerance（IGT） has significant improvement.关键词：impaired glucose tolerance（IGT）;traditional medical formula jiangtangle;traditional Chinese medicine therapy
- 0
- |
- 142
- |
- 6
发布时间：2024-09-23 - 摘要：Objective: To analyze the unreasonable use of outpatient prescription to guide clinical rational drug use. Method : Randomly check of 10,000 outpatient prescription in our hospital during July 2009 to December 2009, then analyze and classified statistics according to the instructions medicines, clinical pharmacology knowledge, and the published literature on the unreasonable prescription. Result : The total of 381 prescriptions was unreasonable. Some of reason was inappropriate choice of solvent, some was that the law did not meet the pharmacokinetics, some was pharmacological antagonism, some was duplicate medication, some was unreasonable to select drug, the others was adding of toxicity and changes in physical and chemical properties. Conclusion : The use of drugs is basically reasonable in our hospital, but we found a few problems.So we hope that physicians and pharmacists to strengthen the pharmaceutical knowledge in common.关键词：prescription;irrational use for drugs;analysis
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:观察中药长皮膏对高位复杂性肛瘘术后创面愈合的临床疗效。方法:将60例高位复杂性肛瘘术后患者随机分组,治疗组（30例）采用中药长皮膏纱布外敷,对照组（30例）采用凡士林纱布外敷,对两组高位复杂性肛瘘术后创面愈合情况进行比较,并对患者的术后症状（如疼痛、水肿、渗液、疤痕等）以及疗程进行观察和检测。结果:治疗组患者的治愈时间为（25.17±4.61）d,对照组为（28.57±3.11）d,治疗组短于对照组（P<0.01）。两组患者术后第5天疼痛、水肿、渗液情况比较差异不显著;术后第10天,治疗组患者疼痛、水肿、渗液情况明显好转,与对照组比,差异显著（P<0.05）;创面完全治愈后,治疗组患者疤痕面积为（0.22±0.06）cm2,对照组为（0.25±0.05）cm2,两者相比差异也有统计学意义（P<0.05）。结论:中药长皮膏对促进高位复杂性肛瘘术后创面的愈合,具有疗程短、痛苦小、疤痕少的优点。关键词：中药长皮膏;高位复杂性肛瘘术后;创面;临床疗效
- 0
- |
- 140
- |
- 11
发布时间：2024-09-23 - 摘要：Related literature about drug bioavailability was retrieved and analyzed, and the article reviewed from different aspects, such as the definition and classification of the bioavailability, the suggestion and development of it, and the influencing factors on oral drug bioavailability and the methods to improve it.关键词：bioavailability;biological utilization degree;biological utilization rate
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：For further detailed the medicinal and efficacy of Lonicerae japonicae flos,the past dynasties ancient books and authoritative pharmic monograph were collected,sorted,compared and analysised,and combined with the modern study on Lonicerae japonicae flos.herbal medicinal textual research on character of medicine and efficacy of Lonicerae japonicae flos.The results showed that the medicinal and efficacy materia medica records were not the same in past dynasties of honeysuckle,and summarized mainstream view that the character of Lonicerae japonicae flos was cold,and the efficacy was clearing heat and detoxicating.关键词：Lonicerae Japonicae Flos;textual;character of medicine
- 0
- |
- 1414
- |
- 41
发布时间：2024-09-23 - 摘要：Objective:To analyze the unreasonable use of outpatient prescription to guide clinical rational drug use.Method: Randomly check of 10,000 outpatient prescription in our hospital during July 2009 to December 2009,then analyze and classified statistics according to the instructions medicines,clinical pharmacology knowledge,and the published literature on the unreasonable prescription.Result: The total of 381 prescriptions was unreasonable.Some of reason was inappropriate choice of solvent,some was that the law did not meet the pharmacokinetics,some was pharmacological antagonism,some was duplicate medication,some was unreasonable to select drug,the others was adding of toxicity and changes in physical and chemical properties.Conclusion: The use of drugs is basically reasonable in our hospital,but we found a few problems.So we hope that physicians and pharmacists to strengthen the pharmaceutical knowledge in common.关键词：prescription;irrational use for drugs;analysis
- 0
- |
- 48
- |
- 6
发布时间：2024-09-23 - 摘要：Related literature about drug bioavailability was retrieved and analyzed,and the article reviewed from different aspects,such as the definition and classification of the bioavailability,the suggestion and development of it,and the influencing factors on oral drug bioavailability and the methods to improve it.关键词：bioavailability;biological utilization degree;biological utilization rate
- 0
- |
- 2105
- |
- 59
发布时间：2024-09-23 - 摘要：<正>神经性皮炎常呈慢性病程,多年不愈,且极易复发,治疗较为棘手。笔者于2007年5月～2009年4月用当归饮子加味治疗神经性皮炎65例,获得了较好疗效。1临床资料1.1一般资料观察病例为我院门诊患者,共130例,随机分为两组,每组65例。治疗组男35例,女30例,年龄16～
- 0
- |
- 194
- |
- 10
发布时间：2024-09-23 - 摘要：<正>急性脑梗死是临床常见病、多发病,本文应用补阳还五汤加减联合西药治疗急性脑梗死,取得了不错的临床疗效。1资料与方法1.1一般资料全部病例均为我院2006年6月～2009年12月神经内科住院急性脑梗死患者,所有病例均符合1995年中华医学会第4次脑血管病学术会议制定的《各类脑血管
- 0
- |
- 41
- |
- 10
发布时间：2024-09-23 - 摘要：<正>中药调剂,是指中药房、中药店的调剂人员根据医师处方要求,按照配方程序和原则,及时、准确地调配和发售中药饮片或中成药的一项操作技术。调剂工作,是一项复杂细致的工作,它直接关系患者的病程和安危。中药调剂工作,一般可分为审方,计价,调配,复核及给药5个程序,其中调配为操作中的重要环节[1]。笔者就我院药房饮片调配中出现的差错进行统计分析。
- 0
- |
- 134
- |
- 16
发布时间：2024-09-23 - 摘要：<正>医患纠纷事件的频繁发生,特别是近年来,"医闹"的出现使这问题更为突出,医患关系成为社会关注的焦点问题。医患矛盾出现激化趋势,严重扰乱了正常的医疗秩序,同时也制约了医疗事业的发展。如何采取有效的措施防范医疗纠纷,防止医患矛盾的扩大化,日益为众多医疗机构所关注。笔者就我院在2007～2009年间发生的医患纠纷原因进行分析,提出防范措施和处理对策。
- 0
- |
- 179
- |
- 8
发布时间：2024-09-23 - 摘要：<正>黄褐斑是一种颜面部出现局限性色素沉着的皮肤病。主要表现面部皮肤出现大小不等、形状不一的色素斑片,颜色多为淡褐色、黄褐色,也有咖啡色或淡黑色。以眼部下外侧多见,亦对称分布于前额、颧、眉、鼻、上唇、口周、颏等处,边缘清楚或呈弥漫性,表面皮肤光滑,不痛不痒,无皮屑,抚之不碍手。多见于中青年女性。病程长,难治愈。从气血理论论治黄褐斑是本文探讨的重点。
- 0
- |
- 188
- |
- 6
发布时间：2024-09-23
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0